AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene38376

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene38376.t1 MTR_3g102800 92.945 326 5 1 1 308 1 326 0.0 618
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene38376.t1 AT5G65860 71.739 322 71 2 4 305 5 326 4.61e-177 492
MS.gene38376.t1 AT5G65860 71.739 322 71 2 4 305 5 326 4.61e-177 492
MS.gene38376.t1 AT5G65860 71.739 322 71 2 4 305 25 346 1.55e-176 492

Find 96 sgRNAs with CRISPR-Local

Find 158 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCTCTTGAGCTTGAGAATT+TGG 0.148087 3.4:+90517499 MS.gene38376:CDS
TGATTCCGGTTATGATTATT+TGG 0.213704 3.4:+90516150 MS.gene38376:CDS
CATGTACTCAATATTGGATT+TGG 0.261666 3.4:+90516292 MS.gene38376:CDS
TCTCTTGAGCTTGAGAATTT+GGG 0.285212 3.4:+90517500 MS.gene38376:CDS
ATTGCCTGTTAAGGATTGTT+TGG 0.285893 3.4:+90517544 MS.gene38376:CDS
TTGCCTGTTAAGGATTGTTT+GGG 0.287460 3.4:+90517545 MS.gene38376:CDS
CTATGCGAGGCGGCGATTAA+AGG 0.287831 3.4:+90515590 MS.gene38376:CDS
GTTATGATTATTTGGAAGAT+AGG 0.317017 3.4:+90516158 MS.gene38376:CDS
TTCTCTCCGCCGGTGCTCCT+TGG 0.327627 3.4:+90515735 MS.gene38376:CDS
ACGTATGCTTCGCACGGGTT+GGG 0.340731 3.4:+90516396 MS.gene38376:CDS
TCTTTCTTCAATGGCCTTTG+TGG 0.351869 3.4:+90517437 MS.gene38376:CDS
ATTAATTCCATTGCCTGTTA+AGG 0.360892 3.4:+90517535 MS.gene38376:CDS
CATGCTAAAGCTGTTTGTTC+AGG 0.364371 3.4:+90516262 MS.gene38376:CDS
GGTGGTCATGTACTCAATAT+TGG 0.375114 3.4:+90516286 MS.gene38376:CDS
CTCAATATTGGATTTGGAAT+GGG 0.395612 3.4:+90516298 MS.gene38376:CDS
GGAATATACTCTTTCTTCAA+TGG 0.395895 3.4:+90517428 MS.gene38376:CDS
TCATCTGCAGACTGACAAAC+AGG 0.396199 3.4:-90517626 None:intergenic
AACGTATGCTTCGCACGGGT+TGG 0.424986 3.4:+90516395 MS.gene38376:CDS
TGCTTTGCGGCGAGCATAAG+AGG 0.429396 3.4:-90515686 None:intergenic
ATCTTCCAAATAATCATAAC+CGG 0.430063 3.4:-90516155 None:intergenic
GAAAAGAAAAGCACTGATTC+CGG 0.430789 3.4:+90516136 MS.gene38376:CDS
CCATGTTGTTTACTGCCACT+TGG 0.440964 3.4:+90517475 MS.gene38376:CDS
CGTATGCTTCGCACGGGTTG+GGG 0.445579 3.4:+90516397 MS.gene38376:CDS
GGGATATTCTTTGACACCTA+CGG 0.456919 3.4:+90517344 MS.gene38376:intron
TTAGCATGAGCCTCCATCAA+TGG 0.470308 3.4:-90516247 None:intergenic
GGGTGCATGGCCGTGCTTTG+CGG 0.480399 3.4:-90515699 None:intergenic
CCACCCGGCTTCAACAATAC+AGG 0.482020 3.4:-90517407 None:intergenic
GGATATTCTTTGACACCTAC+GGG 0.485801 3.4:+90517345 MS.gene38376:CDS
ACTCAATATTGGATTTGGAA+TGG 0.488555 3.4:+90516297 MS.gene38376:CDS
TTCTTCAATGGCCTTTGTGG+TGG 0.493941 3.4:+90517440 MS.gene38376:CDS
GTTGGGAACAATTGCTAGGA+AGG 0.504005 3.4:+90516114 MS.gene38376:CDS
TTATGATTATTTGGAAGATA+GGG 0.505378 3.4:+90516159 MS.gene38376:CDS
GGAGAGAAGGATGTCGAGGA+TGG 0.507596 3.4:-90515720 None:intergenic
CTCGACATCCTTCTCTCCGC+CGG 0.511422 3.4:+90515725 MS.gene38376:CDS
TCAACAATGGTATGTTTCAC+AGG 0.511587 3.4:-90516352 None:intergenic
AAGGTGTCAAACACAGATAC+TGG 0.517912 3.4:+90517582 MS.gene38376:CDS
TCTCCCTGTATTGTTGAAGC+CGG 0.518751 3.4:+90517403 MS.gene38376:CDS
GGCATGGGAGAAGCCATTGA+TGG 0.521455 3.4:+90516234 MS.gene38376:CDS
CATATGTTTCAAGCTGAGAC+AGG 0.523440 3.4:-90516465 None:intergenic
TCTCCTTCCAACCTTTCCGC+CGG 0.523539 3.4:+90515767 MS.gene38376:CDS
CTCTTGAGCTTGAGAATTTG+GGG 0.525010 3.4:+90517501 MS.gene38376:CDS
AAACAATCCTTAACAGGCAA+TGG 0.525589 3.4:-90517542 None:intergenic
CTCCCTGTATTGTTGAAGCC+GGG 0.525798 3.4:+90517404 MS.gene38376:CDS
GTTACTCATTTCGACGGCGA+TGG 0.526133 3.4:+90515656 MS.gene38376:CDS
AAAGCTGTTTGTTCAGGAGG+TGG 0.540005 3.4:+90516268 MS.gene38376:CDS
AAGGAGCACCGGCGGAGAGA+AGG 0.543782 3.4:-90515733 None:intergenic
TAGTGTTGGGAACAATTGCT+AGG 0.547152 3.4:+90516110 MS.gene38376:intron
AGCATACGTTCATAAACCTC+AGG 0.548913 3.4:-90516382 None:intergenic
GCTAAAGCTGTTTGTTCAGG+AGG 0.553788 3.4:+90516265 MS.gene38376:CDS
TCTCAGCTTGAAACATATGA+TGG 0.557266 3.4:+90516469 MS.gene38376:CDS
TCAACAATACAGGGAGATGT+TGG 0.557688 3.4:-90517397 None:intergenic
TTGCGAAATCACCGGCGGAA+AGG 0.562943 3.4:-90515778 None:intergenic
GGATGTCGAGGATGGGTGCA+TGG 0.563108 3.4:-90515712 None:intergenic
GATTAAAGGTGAGACGGAGA+AGG 0.572642 3.4:+90515604 MS.gene38376:CDS
CTTATGCTCGCCGCAAAGCA+CGG 0.574060 3.4:+90515689 MS.gene38376:CDS
GAGAGAAGGATGTCGAGGAT+GGG 0.580848 3.4:-90515719 None:intergenic
GAAATGAGTAACGTCGGCGC+CGG 0.581725 3.4:-90515645 None:intergenic
GAAATCACCGGCGGAAAGGT+TGG 0.582456 3.4:-90515774 None:intergenic
TTCTTGCATTGCGAAATCAC+CGG 0.583608 3.4:-90515786 None:intergenic
GCCGACGTTACTCATTTCGA+CGG 0.583836 3.4:+90515650 MS.gene38376:CDS
AAGAGCGTTCCAAGGAGCAC+CGG 0.584379 3.4:-90515744 None:intergenic
CACCCGGCTTCAACAATACA+GGG 0.584983 3.4:-90517406 None:intergenic
ACAATACAGGGAGATGTTGG+TGG 0.586371 3.4:-90517394 None:intergenic
GAAGGAGAAAGAGCGTTCCA+AGG 0.591032 3.4:-90515752 None:intergenic
TCGCACGGGTTGGGGTGAGA+AGG 0.593861 3.4:+90516405 MS.gene38376:CDS
CATTGTTGAGGCTCATCCTG+AGG 0.595016 3.4:+90516366 MS.gene38376:CDS
GTAAAGTCTCTTATTGACTC+CGG 0.598492 3.4:+90515626 MS.gene38376:CDS
AGCGTTCCAAGGAGCACCGG+CGG 0.601767 3.4:-90515741 None:intergenic
TGAGAGTAAAGCTGTGATGA+TGG 0.605332 3.4:+90516213 MS.gene38376:CDS
TATGAACGTATGCTTCGCAC+GGG 0.607053 3.4:+90516391 MS.gene38376:CDS
TCACCGGCGGAAAGGTTGGA+AGG 0.609268 3.4:-90515770 None:intergenic
CCAAGTGGCAGTAAACAACA+TGG 0.611294 3.4:-90517475 None:intergenic
GGTGATTTCGCAATGCAAGA+AGG 0.613606 3.4:+90515788 MS.gene38376:CDS
TTATGAACGTATGCTTCGCA+CGG 0.616186 3.4:+90516390 MS.gene38376:CDS
CCTGTATTGTTGAAGCCGGG+TGG 0.618155 3.4:+90517407 MS.gene38376:CDS
GTAAAGCTGTGATGATGGCA+TGG 0.618846 3.4:+90516218 MS.gene38376:CDS
CGGCGGAGAGAAGGATGTCG+AGG 0.626647 3.4:-90515724 None:intergenic
AAGAAAGAGTATATTCCACC+CGG 0.629337 3.4:-90517422 None:intergenic
TGCCTGTTAAGGATTGTTTG+GGG 0.632646 3.4:+90517546 MS.gene38376:CDS
GGCGGCGATTAAAGGTGAGA+CGG 0.637047 3.4:+90515598 MS.gene38376:CDS
ATGGGAGAAGCCATTGATGG+AGG 0.639979 3.4:+90516237 MS.gene38376:CDS
GCCTGTTAAGGATTGTTTGG+GGG 0.641124 3.4:+90517547 MS.gene38376:CDS
AGGCAATGGAATTAATTGTG+TGG 0.643792 3.4:-90517528 None:intergenic
GAAGGAAGAAGAACTATGCG+AGG 0.647053 3.4:+90515577 MS.gene38376:CDS
CTCAGGATGAGCCTCAACAA+TGG 0.655173 3.4:-90516365 None:intergenic
TGTGAAACATACCATTGTTG+AGG 0.661097 3.4:+90516354 MS.gene38376:CDS
TAAAGCTGTGATGATGGCAT+GGG 0.687762 3.4:+90516219 MS.gene38376:CDS
TTGCATTGCGAAATCACCGG+CGG 0.690041 3.4:-90515783 None:intergenic
GCCGTCGAAATGAGTAACGT+CGG 0.693746 3.4:-90515651 None:intergenic
AAAATGCATTACCACCACAA+AGG 0.694739 3.4:-90517451 None:intergenic
CAAGCTCAAGAGATACCAAG+TGG 0.699247 3.4:-90517490 None:intergenic
GGAAGAAGAACTATGCGAGG+CGG 0.711413 3.4:+90515580 MS.gene38376:CDS
CATCTGCAGACTGACAAACA+GGG 0.712802 3.4:-90517625 None:intergenic
GTCGTCATAGTACTCCCCGT+AGG 0.723736 3.4:-90517360 None:intergenic
GATATTCTTTGACACCTACG+GGG 0.737280 3.4:+90517346 MS.gene38376:CDS
CTGCAGACTGACAAACAGGG+AGG 0.782216 3.4:-90517622 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAGAAAATCAAAACAAA+TGG - chr3.4:90517322-90517341 None:intergenic 15.0%
!!! TTTTTGTATTTTACTTTGTT+AGG + chr3.4:90516073-90516092 MS.gene38376:intron 15.0%
!! AAAAAAAAAACAGCAGTAAT+AGG + chr3.4:90516779-90516798 MS.gene38376:intron 20.0%
!! AAAAAAAAACAGCAGTAATA+GGG + chr3.4:90516780-90516799 MS.gene38376:intron 20.0%
!! TTATGATTATTTGGAAGATA+GGG + chr3.4:90516159-90516178 MS.gene38376:CDS 20.0%
!!! AATAGAATTGATTTCGTAAA+AGG + chr3.4:90516621-90516640 MS.gene38376:intron 20.0%
!!! TCATTTTCACAAATTATTAC+CGG - chr3.4:90515922-90515941 None:intergenic 20.0%
!!! TGTTTTGATTTTCTTTTTCA+GGG + chr3.4:90517324-90517343 MS.gene38376:intron 20.0%
!!! TTGTTTTGATTTTCTTTTTC+AGG + chr3.4:90517323-90517342 MS.gene38376:intron 20.0%
!!! TTTTAGTGAAGATAAAGTAA+TGG + chr3.4:90516186-90516205 MS.gene38376:CDS 20.0%
! AACACTTAATGTTAAAAAGC+CGG + chr3.4:90517094-90517113 MS.gene38376:intron 25.0%
! AACTTAAATACAACTGCTTA+TGG + chr3.4:90516648-90516667 MS.gene38376:intron 25.0%
! ATCTTCCAAATAATCATAAC+CGG - chr3.4:90516158-90516177 None:intergenic 25.0%
! ATTAAAAGTAGCATCTATGT+TGG - chr3.4:90516943-90516962 None:intergenic 25.0%
! GTTATGATTATTTGGAAGAT+AGG + chr3.4:90516158-90516177 MS.gene38376:CDS 25.0%
! TAACCACATAAATTTCACAA+AGG - chr3.4:90516832-90516851 None:intergenic 25.0%
! TGTTTAACACTAACATGTAT+TGG + chr3.4:90515983-90516002 MS.gene38376:intron 25.0%
! TTAGTGAAAATACATGTCTT+TGG - chr3.4:90517018-90517037 None:intergenic 25.0%
! TTGCTTATGTGAAAAACATA+GGG + chr3.4:90517222-90517241 MS.gene38376:intron 25.0%
! TTTCACAAAGGAAAAAACTT+AGG - chr3.4:90516820-90516839 None:intergenic 25.0%
! TTTCCTTTGTGAAATTTATG+TGG + chr3.4:90516826-90516845 MS.gene38376:intron 25.0%
! TTTGCTTATGTGAAAAACAT+AGG + chr3.4:90517221-90517240 MS.gene38376:intron 25.0%
!! GAAAATGTTCAGATCATTTT+TGG + chr3.4:90516427-90516446 MS.gene38376:CDS 25.0%
!! GGTTACAATTTAGTTTCTAA+GGG + chr3.4:90516847-90516866 MS.gene38376:intron 25.0%
!! TCTAATCAAACTAAGTATTG+AGG + chr3.4:90516967-90516986 MS.gene38376:intron 25.0%
!! TGGTTACAATTTAGTTTCTA+AGG + chr3.4:90516846-90516865 MS.gene38376:intron 25.0%
!!! AAGTGTTTTTGTTTTATCAG+TGG - chr3.4:90517081-90517100 None:intergenic 25.0%
ACACTTAATGTTAAAAAGCC+GGG + chr3.4:90517095-90517114 MS.gene38376:intron 30.0%
ACTCAATATTGGATTTGGAA+TGG + chr3.4:90516297-90516316 MS.gene38376:CDS 30.0%
ATAGTTAATTAGACACAGAG+TGG - chr3.4:90515863-90515882 None:intergenic 30.0%
ATTAATTCCATTGCCTGTTA+AGG + chr3.4:90517535-90517554 MS.gene38376:CDS 30.0%
ATTCGATCTGAATTAGTGTT+GGG + chr3.4:90516097-90516116 MS.gene38376:intron 30.0%
ATTTCAGCACTAGAATTGAT+TGG + chr3.4:90516547-90516566 MS.gene38376:intron 30.0%
CATGTACTCAATATTGGATT+TGG + chr3.4:90516292-90516311 MS.gene38376:CDS 30.0%
GGAATATACTCTTTCTTCAA+TGG + chr3.4:90517428-90517447 MS.gene38376:CDS 30.0%
TAAGGGAAGTTGTTTATGAT+TGG + chr3.4:90516864-90516883 MS.gene38376:intron 30.0%
TATTCGATCTGAATTAGTGT+TGG + chr3.4:90516096-90516115 MS.gene38376:intron 30.0%
TGATTCCGGTTATGATTATT+TGG + chr3.4:90516150-90516169 MS.gene38376:CDS 30.0%
TGCTGAAATTAAACATGTAC+TGG - chr3.4:90516536-90516555 None:intergenic 30.0%
TTATGTGAAAAACATAGGGA+AGG + chr3.4:90517226-90517245 MS.gene38376:intron 30.0%
TTTCAGCACTAGAATTGATT+GGG + chr3.4:90516548-90516567 MS.gene38376:intron 30.0%
! CTCAATATTGGATTTGGAAT+GGG + chr3.4:90516298-90516317 MS.gene38376:CDS 30.0%
! TACTATTTTGCAACGGTATT+TGG + chr3.4:90517147-90517166 MS.gene38376:intron 30.0%
AAAATGCATTACCACCACAA+AGG - chr3.4:90517454-90517473 None:intergenic 35.0%
AAACAATCCTTAACAGGCAA+TGG - chr3.4:90517545-90517564 None:intergenic 35.0%
ACAATCCAGTTACAATTACC+AGG - chr3.4:90517276-90517295 None:intergenic 35.0%
AGGCAATGGAATTAATTGTG+TGG - chr3.4:90517531-90517550 None:intergenic 35.0%
ATAAGCAAAACTTGCATTCC+TGG - chr3.4:90517210-90517229 None:intergenic 35.0%
ATCTCTTGAGCTTGAGAATT+TGG + chr3.4:90517499-90517518 MS.gene38376:CDS 35.0%
ATTGCCTGTTAAGGATTGTT+TGG + chr3.4:90517544-90517563 MS.gene38376:CDS 35.0%
TAATGCCTGGTAATTGTAAC+TGG + chr3.4:90517268-90517287 MS.gene38376:intron 35.0%
TCAACAATGGTATGTTTCAC+AGG - chr3.4:90516355-90516374 None:intergenic 35.0%
TCACTAACATCACAGTTGAT+AGG + chr3.4:90517031-90517050 MS.gene38376:intron 35.0%
TCAGTGGATAGTATCAGAAT+TGG - chr3.4:90517065-90517084 None:intergenic 35.0%
TCTCAGCTTGAAACATATGA+TGG + chr3.4:90516469-90516488 MS.gene38376:CDS 35.0%
TCTCTTGAGCTTGAGAATTT+GGG + chr3.4:90517500-90517519 MS.gene38376:CDS 35.0%
TGTGAAACATACCATTGTTG+AGG + chr3.4:90516354-90516373 MS.gene38376:CDS 35.0%
TGTGTGACTTAAGAATTACC+CGG - chr3.4:90517116-90517135 None:intergenic 35.0%
TTGCCTGTTAAGGATTGTTT+GGG + chr3.4:90517545-90517564 MS.gene38376:CDS 35.0%
! AAGAAAGAGTATATTCCACC+CGG - chr3.4:90517425-90517444 None:intergenic 35.0%
! TGTGAGCTACTATTTTGCAA+CGG + chr3.4:90517140-90517159 MS.gene38376:intron 35.0%
!! GAAAAGAAAAGCACTGATTC+CGG + chr3.4:90516136-90516155 MS.gene38376:CDS 35.0%
!! GTAAAGTCTCTTATTGACTC+CGG + chr3.4:90515626-90515645 MS.gene38376:CDS 35.0%
!!! TTCAGATCATTTTTGGTCGT+TGG + chr3.4:90516434-90516453 MS.gene38376:CDS 35.0%
AGCATACGTTCATAAACCTC+AGG - chr3.4:90516385-90516404 None:intergenic 40.0%
ATTGTTTGGGGGAAGAAGTT+TGG + chr3.4:90517558-90517577 MS.gene38376:CDS 40.0%
CATATGTTTCAAGCTGAGAC+AGG - chr3.4:90516468-90516487 None:intergenic 40.0%
CATGCTAAAGCTGTTTGTTC+AGG + chr3.4:90516262-90516281 MS.gene38376:CDS 40.0%
CTCTTGAGCTTGAGAATTTG+GGG + chr3.4:90517501-90517520 MS.gene38376:CDS 40.0%
GAAGAAGCTCATAAGCTTCT+TGG - chr3.4:90515817-90515836 None:intergenic 40.0%
GACACAGAGTGGTTTGAATT+TGG - chr3.4:90515852-90515871 None:intergenic 40.0%
GATATTCTTTGACACCTACG+GGG + chr3.4:90517346-90517365 MS.gene38376:CDS 40.0%
GGATATTCTTTGACACCTAC+GGG + chr3.4:90517345-90517364 MS.gene38376:CDS 40.0%
GGGATATTCTTTGACACCTA+CGG + chr3.4:90517344-90517363 MS.gene38376:intron 40.0%
GGTGGTCATGTACTCAATAT+TGG + chr3.4:90516286-90516305 MS.gene38376:CDS 40.0%
GTTTGTTTGGTTGTAATGCC+TGG + chr3.4:90517255-90517274 MS.gene38376:intron 40.0%
TATGAGCTTCTTCTCAATGC+TGG + chr3.4:90515824-90515843 MS.gene38376:intron 40.0%
TCAACAATACAGGGAGATGT+TGG - chr3.4:90517400-90517419 None:intergenic 40.0%
TCTTTCTTCAATGGCCTTTG+TGG + chr3.4:90517437-90517456 MS.gene38376:CDS 40.0%
TGAGAGTAAAGCTGTGATGA+TGG + chr3.4:90516213-90516232 MS.gene38376:CDS 40.0%
TGCCTGTTAAGGATTGTTTG+GGG + chr3.4:90517546-90517565 MS.gene38376:CDS 40.0%
TTATGAACGTATGCTTCGCA+CGG + chr3.4:90516390-90516409 MS.gene38376:CDS 40.0%
TTCTTGCATTGCGAAATCAC+CGG - chr3.4:90515789-90515808 None:intergenic 40.0%
TTGTTTGGGGGAAGAAGTTT+GGG + chr3.4:90517559-90517578 MS.gene38376:CDS 40.0%
! AAGGTGTCAAACACAGATAC+TGG + chr3.4:90517582-90517601 MS.gene38376:CDS 40.0%
! ATGCCTTCCATTAGAAGTGT+CGG + chr3.4:90516009-90516028 MS.gene38376:intron 40.0%
! GGGAAGGTTTTCTGTTTGTT+TGG + chr3.4:90517242-90517261 MS.gene38376:intron 40.0%
! TAGTGTTGGGAACAATTGCT+AGG + chr3.4:90516110-90516129 MS.gene38376:intron 40.0%
!! AATAGCACCGACACTTCTAA+TGG - chr3.4:90516019-90516038 None:intergenic 40.0%
!! TAAAGCTGTGATGATGGCAT+GGG + chr3.4:90516219-90516238 MS.gene38376:CDS 40.0%
AAAGCTGTTTGTTCAGGAGG+TGG + chr3.4:90516268-90516287 MS.gene38376:CDS 45.0%
ACAATACAGGGAGATGTTGG+TGG - chr3.4:90517397-90517416 None:intergenic 45.0%
CAAGCTCAAGAGATACCAAG+TGG - chr3.4:90517493-90517512 None:intergenic 45.0%
CATCTGCAGACTGACAAACA+GGG - chr3.4:90517628-90517647 None:intergenic 45.0%
CCAAGTGGCAGTAAACAACA+TGG - chr3.4:90517478-90517497 None:intergenic 45.0%
GAAGGAAGAAGAACTATGCG+AGG + chr3.4:90515577-90515596 MS.gene38376:CDS 45.0%
GATTAAAGGTGAGACGGAGA+AGG + chr3.4:90515604-90515623 MS.gene38376:CDS 45.0%
GCCTCTAAACTCTTTGACGT+GGG + chr3.4:90517296-90517315 MS.gene38376:intron 45.0%
GCCTGTTAAGGATTGTTTGG+GGG + chr3.4:90517547-90517566 MS.gene38376:CDS 45.0%
GCTAAAGCTGTTTGTTCAGG+AGG + chr3.4:90516265-90516284 MS.gene38376:CDS 45.0%
GGTGATTTCGCAATGCAAGA+AGG + chr3.4:90515788-90515807 MS.gene38376:CDS 45.0%
GTTAGTGTCAGTTTCGTGTC+AGG - chr3.4:90515890-90515909 None:intergenic 45.0%
GTTGGGAACAATTGCTAGGA+AGG + chr3.4:90516114-90516133 MS.gene38376:CDS 45.0%
TATGAACGTATGCTTCGCAC+GGG + chr3.4:90516391-90516410 MS.gene38376:CDS 45.0%
TCATCTGCAGACTGACAAAC+AGG - chr3.4:90517629-90517648 None:intergenic 45.0%
TCCCCCAAACAATCCTTAAC+AGG - chr3.4:90517551-90517570 None:intergenic 45.0%
TCTCCCTGTATTGTTGAAGC+CGG + chr3.4:90517403-90517422 MS.gene38376:CDS 45.0%
TGACACTAACACGTCAACAC+CGG + chr3.4:90515900-90515919 MS.gene38376:intron 45.0%
TGCCTCTAAACTCTTTGACG+TGG + chr3.4:90517295-90517314 MS.gene38376:intron 45.0%
TTAGCATGAGCCTCCATCAA+TGG - chr3.4:90516250-90516269 None:intergenic 45.0%
TTCTTCAATGGCCTTTGTGG+TGG + chr3.4:90517440-90517459 MS.gene38376:CDS 45.0%
! CCATGTTGTTTACTGCCACT+TGG + chr3.4:90517475-90517494 MS.gene38376:CDS 45.0%
!! GTAAAGCTGTGATGATGGCA+TGG + chr3.4:90516218-90516237 MS.gene38376:CDS 45.0%
!! TGCATTCCTGGAAAGCATCT+GGG - chr3.4:90517198-90517217 None:intergenic 45.0%
!! TTGCATTCCTGGAAAGCATC+TGG - chr3.4:90517199-90517218 None:intergenic 45.0%
ATGGGAGAAGCCATTGATGG+AGG + chr3.4:90516237-90516256 MS.gene38376:CDS 50.0%
CACCCGGCTTCAACAATACA+GGG - chr3.4:90517409-90517428 None:intergenic 50.0%
CATTGTTGAGGCTCATCCTG+AGG + chr3.4:90516366-90516385 MS.gene38376:CDS 50.0%
CTCAGGATGAGCCTCAACAA+TGG - chr3.4:90516368-90516387 None:intergenic 50.0%
CTCCCTGTATTGTTGAAGCC+GGG + chr3.4:90517404-90517423 MS.gene38376:CDS 50.0%
CTGTTTCCCAGATGCTTTCC+AGG + chr3.4:90517189-90517208 MS.gene38376:intron 50.0%
GAGAGAAGGATGTCGAGGAT+GGG - chr3.4:90515722-90515741 None:intergenic 50.0%
GCCCACGTCAAAGAGTTTAG+AGG - chr3.4:90517300-90517319 None:intergenic 50.0%
GCCGACGTTACTCATTTCGA+CGG + chr3.4:90515650-90515669 MS.gene38376:CDS 50.0%
GCCGTCGAAATGAGTAACGT+CGG - chr3.4:90515654-90515673 None:intergenic 50.0%
GGAAGAAGAACTATGCGAGG+CGG + chr3.4:90515580-90515599 MS.gene38376:CDS 50.0%
GTTACTCATTTCGACGGCGA+TGG + chr3.4:90515656-90515675 MS.gene38376:CDS 50.0%
TTGCATTGCGAAATCACCGG+CGG - chr3.4:90515786-90515805 None:intergenic 50.0%
TTGGGGGAAGAAGTTTGGGA+AGG + chr3.4:90517563-90517582 MS.gene38376:CDS 50.0%
! GCACCGACACTTCTAATGGA+AGG - chr3.4:90516015-90516034 None:intergenic 50.0%
!! GAAGGAGAAAGAGCGTTCCA+AGG - chr3.4:90515755-90515774 None:intergenic 50.0%
AACGTATGCTTCGCACGGGT+TGG + chr3.4:90516395-90516414 MS.gene38376:CDS 55.0%
ACGTATGCTTCGCACGGGTT+GGG + chr3.4:90516396-90516415 MS.gene38376:CDS 55.0%
CCACCCGGCTTCAACAATAC+AGG - chr3.4:90517410-90517429 None:intergenic 55.0%
CCTGTATTGTTGAAGCCGGG+TGG + chr3.4:90517407-90517426 MS.gene38376:CDS 55.0%
CTATGCGAGGCGGCGATTAA+AGG + chr3.4:90515590-90515609 MS.gene38376:CDS 55.0%
CTGCAGACTGACAAACAGGG+AGG - chr3.4:90517625-90517644 None:intergenic 55.0%
CTTATGCTCGCCGCAAAGCA+CGG + chr3.4:90515689-90515708 MS.gene38376:CDS 55.0%
GAAATCACCGGCGGAAAGGT+TGG - chr3.4:90515777-90515796 None:intergenic 55.0%
GAAATGAGTAACGTCGGCGC+CGG - chr3.4:90515648-90515667 None:intergenic 55.0%
GGAGAGAAGGATGTCGAGGA+TGG - chr3.4:90515723-90515742 None:intergenic 55.0%
GGCATGGGAGAAGCCATTGA+TGG + chr3.4:90516234-90516253 MS.gene38376:CDS 55.0%
GGCGGCGATTAAAGGTGAGA+CGG + chr3.4:90515598-90515617 MS.gene38376:CDS 55.0%
GTCGTCATAGTACTCCCCGT+AGG - chr3.4:90517363-90517382 None:intergenic 55.0%
TCTCCTTCCAACCTTTCCGC+CGG + chr3.4:90515767-90515786 MS.gene38376:CDS 55.0%
TGCTTTGCGGCGAGCATAAG+AGG - chr3.4:90515689-90515708 None:intergenic 55.0%
TTGCGAAATCACCGGCGGAA+AGG - chr3.4:90515781-90515800 None:intergenic 55.0%
!! AAGAGCGTTCCAAGGAGCAC+CGG - chr3.4:90515747-90515766 None:intergenic 55.0%
CTCGACATCCTTCTCTCCGC+CGG + chr3.4:90515725-90515744 MS.gene38376:CDS 60.0%
GGATGTCGAGGATGGGTGCA+TGG - chr3.4:90515715-90515734 None:intergenic 60.0%
TCACCGGCGGAAAGGTTGGA+AGG - chr3.4:90515773-90515792 None:intergenic 60.0%
! CGTATGCTTCGCACGGGTTG+GGG + chr3.4:90516397-90516416 MS.gene38376:CDS 60.0%
CGGCGGAGAGAAGGATGTCG+AGG - chr3.4:90515727-90515746 None:intergenic 65.0%
GGGTGCATGGCCGTGCTTTG+CGG - chr3.4:90515702-90515721 None:intergenic 65.0%
TTCTCTCCGCCGGTGCTCCT+TGG + chr3.4:90515735-90515754 MS.gene38376:CDS 65.0%
!! AAGGAGCACCGGCGGAGAGA+AGG - chr3.4:90515736-90515755 None:intergenic 65.0%
!! AGCGTTCCAAGGAGCACCGG+CGG - chr3.4:90515744-90515763 None:intergenic 65.0%
!! TCGCACGGGTTGGGGTGAGA+AGG + chr3.4:90516405-90516424 MS.gene38376:CDS 65.0%


Chromosome Type Strat End Strand Name
chr3.4 gene 90515575 90517658 90515575 ID=MS.gene38376
chr3.4 mRNA 90515575 90517658 90515575 ID=MS.gene38376.t1;Parent=MS.gene38376
chr3.4 exon 90515575 90515809 90515575 ID=MS.gene38376.t1.exon1;Parent=MS.gene38376.t1
chr3.4 CDS 90515575 90515809 90515575 ID=cds.MS.gene38376.t1;Parent=MS.gene38376.t1
chr3.4 exon 90516113 90516490 90516113 ID=MS.gene38376.t1.exon2;Parent=MS.gene38376.t1
chr3.4 CDS 90516113 90516490 90516113 ID=cds.MS.gene38376.t1;Parent=MS.gene38376.t1
chr3.4 exon 90517345 90517658 90517345 ID=MS.gene38376.t1.exon3;Parent=MS.gene38376.t1
chr3.4 CDS 90517345 90517658 90517345 ID=cds.MS.gene38376.t1;Parent=MS.gene38376.t1
Gene Sequence

>MS.gene38376

ATGAAGGAAGAAGAACTATGCGAGGCGGCGATTAAAGGTGAGACGGAGAAGGTAAAGTCTCTTATTGACTCCGGCGCCGACGTTACTCATTTCGACGGCGATGGTCTCACTCCTCTTATGCTCGCCGCAAAGCACGGCCATGCACCCATCCTCGACATCCTTCTCTCCGCCGGTGCTCCTTGGAACGCTCTTTCTCCTTCCAACCTTTCCGCCGGTGATTTCGCAATGCAAGAAGGTCACCAAGAAGCTTATGAGCTTCTTCTCAATGCTGGTACCAAATTCAAACCACTCTGTGTCTAATTAACTATGCAGCCTGACACGAAACTGACACTAACACGTCAACACCGGTAATAATTTGTGAAAATGACATAATTAAAATTGAACACAATTACATGTGTGTCGCATTTGTGTTTAACACTAACATGTATTGGAACATGCCTTCCATTAGAAGTGTCGGTGCTATTGAGTTATTTTGTTAGAGTAATTCTAGAAGTTCACTTTTTGTATTTTACTTTGTTAGGTATTCGATCTGAATTAGTGTTGGGAACAATTGCTAGGAAGGAAAAGAAAAGCACTGATTCCGGTTATGATTATTTGGAAGATAGGGTAAGTTTTAGTGAAGATAAAGTAATGGATTCTGAGAGTAAAGCTGTGATGATGGCATGGGAGAAGCCATTGATGGAGGCTCATGCTAAAGCTGTTTGTTCAGGAGGTGGTCATGTACTCAATATTGGATTTGGAATGGGTCTTGTTGATACAGCTATACAACAATATTCACCTGTGAAACATACCATTGTTGAGGCTCATCCTGAGGTTTATGAACGTATGCTTCGCACGGGTTGGGGTGAGAAGGAAAATGTTCAGATCATTTTTGGTCGTTGGCAAGATGTCCTGTCTCAGCTTGAAACATATGATGGTAATTGCTAACTCTTGTGTGCATAAACATCAATGTTTTATTCCAGTACATGTTTAATTTCAGCACTAGAATTGATTGGGTTGTCTTTTTAGTGTGTACTTTGAGAAAGCTATTGATAAACATAAGTTCGAATAGAATTGATTTCGTAAAAGGACTAAACTTAAATACAACTGCTTATGGTACTGTTTTTACGTTAATCTCACAAGAAGACTGCTATTTTCTTCTCAAGCGATAGTTTTTTTTAGCACTTTTTCAAATTTGTTGCTTACAGTTATACTATCTTTCAGTAAAAAAAAAACAGCAGTAATAGGGCTTATAGCACATTACCTAAGTTTTTTCCTTTGTGAAATTTATGTGGTTACAATTTAGTTTCTAAGGGAAGTTGTTTATGATTGGATCACTTAACTTTGAAAGTAAGTTTGATATAAAAGTAGTGTTATTTTTCTAATCCAACATAGATGCTACTTTTAATCACTTCTAATCAAACTAAGTATTGAGGTAAAATCAATTTTGTTTGAGAGAAACCAAAGACATGTATTTTCACTAACATCACAGTTGATAGGAAGTTAAACCAATTCTGATACTATCCACTGATAAAACAAAAACACTTAATGTTAAAAAGCCGGGTAATTCTTAAGTCACACACTACTGTGAGCTACTATTTTGCAACGGTATTTGGAAATATGCGAAAGTTGTAGCTGTTTCCCAGATGCTTTCCAGGAATGCAAGTTTTGCTTATGTGAAAAACATAGGGAAGGTTTTCTGTTTGTTTGGTTGTAATGCCTGGTAATTGTAACTGGATTGTGCCTCTAAACTCTTTGACGTGGGCCATTTGTTTTGATTTTCTTTTTCAGGGATATTCTTTGACACCTACGGGGAGTACTATGACGACTTGAGAGAGTTCCACCAACATCTCCCTGTATTGTTGAAGCCGGGTGGAATATACTCTTTCTTCAATGGCCTTTGTGGTGGTAATGCATTTTTCCATGTTGTTTACTGCCACTTGGTATCTCTTGAGCTTGAGAATTTGGGGTATTCCACACAATTAATTCCATTGCCTGTTAAGGATTGTTTGGGGGAAGAAGTTTGGGAAGGTGTCAAACACAGATACTGGCAGCTTGATACATACTACCTCCCTGTTTGTCAGTCTGCAGATGACTCTGAGTGA
Protein sequence

>MS.gene38376.t1

MKEEELCEAAIKGETEKVKSLIDSGADVTHFDGDGLTPLMLAAKHGHAPILDILLSAGAPWNALSPSNLSAGDFAMQEVLGTIARKEKKSTDSGYDYLEDRVSFSEDKVMDSESKAVMMAWEKPLMEAHAKAVCSGGGHVLNIGFGMGLVDTAIQQYSPVKHTIVEAHPEVYERMLRTGWGEKENVQIIFGRWQDVLSQLETYDGIFFDTYGEYYDDLREFHQHLPVLLKPGGIYSFFNGLCGGNAFFHVVYCHLVSLELENLGYSTQLIPLPVKDCLGEEVWEGVKHRYWQLDTYYLPVCQSADDSE