Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene39070.t1 | RHN47818.1 | 86.4 | 88 | 12 | 0 | 1 | 88 | 101 | 188 | 2.00E-33 | 151.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene39070.t1 | G7KSM4 | 86.4 | 88 | 12 | 0 | 1 | 88 | 101 | 188 | 1.4e-33 | 151.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene05415 | MS.gene39070 | 0.811097 | 8.30E-51 | -1.69E-46 |
| MS.gene057021 | MS.gene39070 | 0.800954 | 1.13E-48 | -1.69E-46 |
| MS.gene059584 | MS.gene39070 | 0.809423 | 1.91E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene39070.t1 | MTR_7g090980 | 86.364 | 88 | 12 | 0 | 1 | 88 | 101 | 188 | 1.51e-45 | 154 |
| MS.gene39070.t1 | MTR_1g029630 | 76.136 | 88 | 20 | 1 | 1 | 88 | 100 | 186 | 4.62e-40 | 140 |
| MS.gene39070.t1 | MTR_1g029630 | 76.136 | 88 | 20 | 1 | 1 | 88 | 100 | 186 | 4.71e-40 | 140 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene39070.t1 | AT1G77320 | 59.091 | 88 | 34 | 1 | 1 | 88 | 100 | 185 | 1.81e-29 | 109 |
| MS.gene39070.t1 | AT1G77320 | 59.091 | 88 | 34 | 1 | 1 | 88 | 100 | 185 | 1.90e-29 | 109 |
| MS.gene39070.t1 | AT1G77320 | 59.091 | 88 | 34 | 1 | 1 | 88 | 93 | 178 | 2.08e-29 | 109 |
Find 18 sgRNAs with CRISPR-Local
Find 78 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATAAGGTTCTTCCTTTCTAA+TGG | 0.270445 | 7.4:-17761756 | None:intergenic |
| AACCATTCATAAGTAACTAT+TGG | 0.328810 | 7.4:+17761823 | None:intergenic |
| GATGAATTAATGATAGAAAT+GGG | 0.328981 | 7.4:-17762909 | MS.gene39070:CDS |
| AAGTAACTATTGGTTTCTTC+AGG | 0.330112 | 7.4:+17761833 | None:intergenic |
| TTATGTCTGGCAGTGGGCTT+TGG | 0.347087 | 7.4:-17761860 | MS.gene39070:intron |
| AGATGAATTAATGATAGAAA+TGG | 0.354164 | 7.4:-17762910 | MS.gene39070:CDS |
| TGTTTCTCAAGAGTCATATA+AGG | 0.355726 | 7.4:-17761773 | MS.gene39070:CDS |
| AACCAATAGTTACTTATGAA+TGG | 0.439724 | 7.4:-17761825 | MS.gene39070:CDS |
| ATATACCAGACCTTGTACTC+TGG | 0.475694 | 7.4:+17762822 | None:intergenic |
| AGATCTTTAACCCATTAGAA+AGG | 0.520340 | 7.4:+17761745 | None:intergenic |
| ATGAATTAATGATAGAAATG+GGG | 0.548594 | 7.4:-17762908 | MS.gene39070:CDS |
| TGAATTAATGATAGAAATGG+GGG | 0.563673 | 7.4:-17762907 | MS.gene39070:CDS |
| ACATAGTTTAAATCTGATGA+CGG | 0.568188 | 7.4:+17762867 | None:intergenic |
| TGGGTTTGATGCGGATCAGA+AGG | 0.575165 | 7.4:-17763154 | MS.gene39070:intron |
| TGAATGGTTAAAGCAATGCT+CGG | 0.595248 | 7.4:-17761809 | MS.gene39070:CDS |
| CTGTCAAAATACTTGCATGT+GGG | 0.607556 | 7.4:-17763173 | MS.gene39070:CDS |
| GCTGTCAAAATACTTGCATG+TGG | 0.614749 | 7.4:-17763174 | MS.gene39070:CDS |
| ACTTGCATGTGGGTTTGATG+CGG | 0.673281 | 7.4:-17763163 | MS.gene39070:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAAAAAAAAAAAAAAGTGA+AGG | + | chr7.4:17762772-17762791 | None:intergenic | 10.0% |
| !! | TTAAGAATAATATAAAAACA+TGG | + | chr7.4:17762155-17762174 | None:intergenic | 10.0% |
| !!! | ACAATTTTAATATTTACAAT+AGG | + | chr7.4:17762948-17762967 | None:intergenic | 10.0% |
| !!! | CTTTTTTTTTTTTTTTTGCT+TGG | - | chr7.4:17762775-17762794 | MS.gene39070:intron | 15.0% |
| !! | AGATGAATTAATGATAGAAA+TGG | - | chr7.4:17762029-17762048 | MS.gene39070:intron | 20.0% |
| !! | ATGAATTAATGATAGAAATG+GGG | - | chr7.4:17762031-17762050 | MS.gene39070:intron | 20.0% |
| !! | GATGAATTAATGATAGAAAT+GGG | - | chr7.4:17762030-17762049 | MS.gene39070:intron | 20.0% |
| !!! | AGTGTTTTTATGTTATACAT+TGG | + | chr7.4:17762236-17762255 | None:intergenic | 20.0% |
| !!! | GGTAGTAGTATAAATTTTTT+AGG | - | chr7.4:17762372-17762391 | MS.gene39070:intron | 20.0% |
| !!! | GTGTTTTTATGTTATACATT+GGG | + | chr7.4:17762235-17762254 | None:intergenic | 20.0% |
| !!! | TGTTTTTATGTTATACATTG+GGG | + | chr7.4:17762234-17762253 | None:intergenic | 20.0% |
| !!! | TTAAACCTTAGAATTTTAGA+GGG | + | chr7.4:17761909-17761928 | None:intergenic | 20.0% |
| ! | AACCAATAGTTACTTATGAA+TGG | - | chr7.4:17763114-17763133 | MS.gene39070:intron | 25.0% |
| ! | AACCATTCATAAGTAACTAT+TGG | + | chr7.4:17763119-17763138 | None:intergenic | 25.0% |
| ! | AATTTATCTTGAACTACAGA+TGG | - | chr7.4:17762433-17762452 | MS.gene39070:intron | 25.0% |
| ! | ACATAGTTTAAATCTGATGA+CGG | + | chr7.4:17762075-17762094 | None:intergenic | 25.0% |
| ! | CCAAAAGAAGAGTTAATTAA+CGG | + | chr7.4:17762341-17762360 | None:intergenic | 25.0% |
| ! | GAATAAATAATGCACAATAG+TGG | - | chr7.4:17762688-17762707 | MS.gene39070:intron | 25.0% |
| ! | TGAATTAATGATAGAAATGG+GGG | - | chr7.4:17762032-17762051 | MS.gene39070:intron | 25.0% |
| !! | AAGTTATCAGTATTTTCTCT+TGG | - | chr7.4:17761927-17761946 | MS.gene39070:intron | 25.0% |
| !! | CTACAACAAACATTTTTCTT+AGG | - | chr7.4:17762746-17762765 | MS.gene39070:intron | 25.0% |
| !! | CTTAAACCTTAGAATTTTAG+AGG | + | chr7.4:17761910-17761929 | None:intergenic | 25.0% |
| !! | GTATTTTCTCTTGGAATATT+AGG | - | chr7.4:17761936-17761955 | MS.gene39070:intron | 25.0% |
| !! | GTTTTTATGTTATACATTGG+GGG | + | chr7.4:17762233-17762252 | None:intergenic | 25.0% |
| !! | TGTCATCGTAAAAAATGTTT+TGG | - | chr7.4:17762092-17762111 | MS.gene39070:intron | 25.0% |
| !!! | TCACTGGTTGTATTATTTTA+TGG | - | chr7.4:17762487-17762506 | MS.gene39070:intron | 25.0% |
| AATATACACCATGTTATGTC+TGG | - | chr7.4:17763066-17763085 | MS.gene39070:intron | 30.0% | |
| ATTGAGAGCAAAAAAAGGAA+CGG | + | chr7.4:17761972-17761991 | None:intergenic | 30.0% | |
| GGTTTATCTCCTAAATTGTT+GGG | - | chr7.4:17762796-17762815 | MS.gene39070:intron | 30.0% | |
| GTTTATCTCCTAAATTGTTG+GGG | - | chr7.4:17762797-17762816 | MS.gene39070:intron | 30.0% | |
| TAGGAAGATGAACTTATTAG+TGG | + | chr7.4:17762929-17762948 | None:intergenic | 30.0% | |
| TGGTTTATCTCCTAAATTGT+TGG | - | chr7.4:17762795-17762814 | MS.gene39070:intron | 30.0% | |
| TGTTTCTCAAGAGTCATATA+AGG | - | chr7.4:17763166-17763185 | MS.gene39070:CDS | 30.0% | |
| TTCCAATTGAGAGCAAAAAA+AGG | + | chr7.4:17761977-17761996 | None:intergenic | 30.0% | |
| ! | CCGTTAATTAACTCTTCTTT+TGG | - | chr7.4:17762338-17762357 | MS.gene39070:intron | 30.0% |
| ! | TGCTCATATATTCTGTTTCT+TGG | - | chr7.4:17762850-17762869 | MS.gene39070:CDS | 30.0% |
| ! | TGGTCTATCTCATTTAGTTT+CGG | - | chr7.4:17762870-17762889 | MS.gene39070:CDS | 30.0% |
| !! | AAGTAACTATTGGTTTCTTC+AGG | + | chr7.4:17763109-17763128 | None:intergenic | 30.0% |
| !! | CAAAACCCTCTAAAATTCTA+AGG | - | chr7.4:17761901-17761920 | MS.gene39070:intron | 30.0% |
| !! | CTTAGAATTTTAGAGGGTTT+TGG | + | chr7.4:17761903-17761922 | None:intergenic | 30.0% |
| !! | GTAAAAAGCATCTAGTCTAA+GGG | - | chr7.4:17762293-17762312 | MS.gene39070:intron | 30.0% |
| !! | TGTAAAAAGCATCTAGTCTA+AGG | - | chr7.4:17762292-17762311 | MS.gene39070:intron | 30.0% |
| !!! | TTCCTTTTTTTGCTCTCAAT+TGG | - | chr7.4:17761972-17761991 | MS.gene39070:intron | 30.0% |
| AAATTGTTGGGGATTTGTAG+TGG | - | chr7.4:17762808-17762827 | MS.gene39070:intron | 35.0% | |
| AGATTGCATATGAGTGGAAT+TGG | + | chr7.4:17763000-17763019 | None:intergenic | 35.0% | |
| AGGATAATAATGTGCAACAG+AGG | + | chr7.4:17762208-17762227 | None:intergenic | 35.0% | |
| CTACAAATCCCCAACAATTT+AGG | + | chr7.4:17762808-17762827 | None:intergenic | 35.0% | |
| CTGTCAAAATACTTGCATGT+GGG | - | chr7.4:17761766-17761785 | MS.gene39070:CDS | 35.0% | |
| GTGGCTAATATGACTCTTAT+TGG | - | chr7.4:17762827-17762846 | MS.gene39070:intron | 35.0% | |
| TATGTTATACATTGGGGGAT+AGG | + | chr7.4:17762228-17762247 | None:intergenic | 35.0% | |
| TGAAGCTTATGTTAGTTCAC+TGG | - | chr7.4:17762471-17762490 | MS.gene39070:intron | 35.0% | |
| TGAATGGTTAAAGCAATGCT+CGG | - | chr7.4:17763130-17763149 | MS.gene39070:intron | 35.0% | |
| ! | GGTCTATCTCATTTAGTTTC+GGG | - | chr7.4:17762871-17762890 | MS.gene39070:CDS | 35.0% |
| !! | AGAATTTTAGAGGGTTTTGG+AGG | + | chr7.4:17761900-17761919 | None:intergenic | 35.0% |
| !! | CTTCTTTTGGCTAGTAGTTT+AGG | - | chr7.4:17762351-17762370 | MS.gene39070:intron | 35.0% |
| !!! | AATTTTAGAGGGTTTTGGAG+GGG | + | chr7.4:17761898-17761917 | None:intergenic | 35.0% |
| !!! | GAATTTTAGAGGGTTTTGGA+GGG | + | chr7.4:17761899-17761918 | None:intergenic | 35.0% |
| ATATACCAGACCTTGTACTC+TGG | + | chr7.4:17762120-17762139 | None:intergenic | 40.0% | |
| ATTCATCTATCTTGACCTGC+AGG | + | chr7.4:17762017-17762036 | None:intergenic | 40.0% | |
| CCACTCATATGCAATCTTTG+AGG | - | chr7.4:17763003-17763022 | MS.gene39070:intron | 40.0% | |
| CCTCAAAGATTGCATATGAG+TGG | + | chr7.4:17763006-17763025 | None:intergenic | 40.0% | |
| GCTGTCAAAATACTTGCATG+TGG | - | chr7.4:17761765-17761784 | MS.gene39070:CDS | 40.0% | |
| TTCATCTATCTTGACCTGCA+GGG | + | chr7.4:17762016-17762035 | None:intergenic | 40.0% | |
| TTTAGTCCTTAATGCGCTGT+TGG | - | chr7.4:17762723-17762742 | MS.gene39070:intron | 40.0% | |
| TTTATTGTATGTGACCCTGC+AGG | - | chr7.4:17761999-17762018 | MS.gene39070:intron | 40.0% | |
| ! | ATCTCATTTAGTTTCGGGCT+TGG | - | chr7.4:17762876-17762895 | MS.gene39070:CDS | 40.0% |
| !! | AAAAGCATCTAGTCTAAGGG+AGG | - | chr7.4:17762296-17762315 | MS.gene39070:intron | 40.0% |
| ACCATGTTATGTCTGGCAGT+GGG | - | chr7.4:17763073-17763092 | MS.gene39070:intron | 45.0% | |
| ACTTGCATGTGGGTTTGATG+CGG | - | chr7.4:17761776-17761795 | MS.gene39070:CDS | 45.0% | |
| AGTCTAAGGGAGGAAGATTC+TGG | - | chr7.4:17762306-17762325 | MS.gene39070:intron | 45.0% | |
| GAGAGCAAAAAAAGGAACGG+AGG | + | chr7.4:17761969-17761988 | None:intergenic | 45.0% | |
| TTGTAGCCAACAGCGCATTA+AGG | + | chr7.4:17762732-17762751 | None:intergenic | 45.0% | |
| !!! | TGTTTTGGCTCCAGAGTACA+AGG | - | chr7.4:17762107-17762126 | MS.gene39070:intron | 45.0% |
| CACCATGTTATGTCTGGCAG+TGG | - | chr7.4:17763072-17763091 | MS.gene39070:intron | 50.0% | |
| TGGGTTTGATGCGGATCAGA+AGG | - | chr7.4:17761785-17761804 | MS.gene39070:CDS | 50.0% | |
| ! | TTATGTCTGGCAGTGGGCTT+TGG | - | chr7.4:17763079-17763098 | MS.gene39070:intron | 50.0% |
| GCCCACTGCCAGACATAACA+TGG | + | chr7.4:17763077-17763096 | None:intergenic | 55.0% | |
| TGGCTCCAGAGTACAAGGTC+TGG | - | chr7.4:17762112-17762131 | MS.gene39070:intron | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 17761759 | 17763202 | 17761759 | ID=MS.gene39070 |
| chr7.4 | mRNA | 17761759 | 17763202 | 17761759 | ID=MS.gene39070.t1;Parent=MS.gene39070 |
| chr7.4 | exon | 17763155 | 17763202 | 17763155 | ID=MS.gene39070.t1.exon1;Parent=MS.gene39070.t1 |
| chr7.4 | CDS | 17763155 | 17763202 | 17763155 | ID=cds.MS.gene39070.t1;Parent=MS.gene39070.t1 |
| chr7.4 | exon | 17762833 | 17762940 | 17762833 | ID=MS.gene39070.t1.exon2;Parent=MS.gene39070.t1 |
| chr7.4 | CDS | 17762833 | 17762940 | 17762833 | ID=cds.MS.gene39070.t1;Parent=MS.gene39070.t1 |
| chr7.4 | exon | 17761759 | 17761869 | 17761759 | ID=MS.gene39070.t1.exon3;Parent=MS.gene39070.t1 |
| chr7.4 | CDS | 17761759 | 17761869 | 17761759 | ID=cds.MS.gene39070.t1;Parent=MS.gene39070.t1 |
| Gene Sequence |
| Protein sequence |
