AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene057021


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene057021.t1 MTR_8g047190 84.211 38 6 0 22 59 54 91 1.24e-16 68.2
MS.gene057021.t1 MTR_0048s0080 49.091 55 28 0 25 79 281 335 1.26e-11 58.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 18 sgRNAs with CRISPR-Local

Find 55 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACCTTCCAGTGATGAACTTT+TGG 0.248742 2.1:-4623051 MS.gene057021:CDS
TTTCCCAACGGTTTGCTTGC+TGG 0.258707 2.1:+4622286 None:intergenic
CAAGCAAACCGTTGGGAAAA+AGG 0.271238 2.1:-4622282 MS.gene057021:CDS
TGTGCGATCACCATTGAGTT+TGG 0.378466 2.1:-4622235 MS.gene057021:CDS
ATCCCTTCGACACTCTCAAC+TGG 0.444586 2.1:+4622259 None:intergenic
TGAAGATGCAATGCAGCTAA+AGG 0.495947 2.1:-4623095 None:intergenic
AACAGCCAAAAGTTCATCAC+TGG 0.531595 2.1:+4623046 None:intergenic
TACTCCAGCAAGCAAACCGT+TGG 0.534254 2.1:-4622290 MS.gene057021:CDS
TGCTACTAAAGAGTCGAAGA+TGG 0.549976 2.1:-4622203 MS.gene057021:CDS
ATCACCATTGAGTTTGGAGA+AGG 0.557143 2.1:-4622229 MS.gene057021:CDS
ACTCCAGCAAGCAAACCGTT+GGG 0.557480 2.1:-4622289 MS.gene057021:CDS
AGAGCCTTCTCCAAACTCAA+TGG 0.564999 2.1:+4622225 None:intergenic
TGATTCAAGCAACAAGGGAG+AGG 0.570500 2.1:-4622329 MS.gene057021:CDS
AGGCCAGTTGAGAGTGTCGA+AGG 0.575521 2.1:-4622262 MS.gene057021:CDS
AGGAGGTGATTCAAGCAACA+AGG 0.594893 2.1:-4622335 MS.gene057021:CDS
GCCAAAAGTTCATCACTGGA+AGG 0.607816 2.1:+4623050 None:intergenic
GGCCAGTTGAGAGTGTCGAA+GGG 0.650645 2.1:-4622261 MS.gene057021:CDS
GGAGGTGATTCAAGCAACAA+GGG 0.650941 2.1:-4622334 MS.gene057021:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GTTAAAAAATTTCTTAAAAA+AGG + chr2.1:4622573-4622592 None:intergenic 10.0%
!!! TAACATTGTTTTATTTTTGA+AGG - chr2.1:4622589-4622608 MS.gene057021:intron 15.0%
!!! TTTATGCTTCATTTTAATTT+TGG - chr2.1:4622897-4622916 MS.gene057021:intron 15.0%
!! AAACATTGAATATCATAAAC+AGG + chr2.1:4622307-4622326 None:intergenic 20.0%
!! AAATCAATTGCTATGAAAAT+TGG + chr2.1:4622472-4622491 None:intergenic 20.0%
!! AATTAAGAATTAAAGTGAGA+TGG + chr2.1:4622741-4622760 None:intergenic 20.0%
!! CTATGAAAGTAAATATAGTT+TGG - chr2.1:4622662-4622681 MS.gene057021:intron 20.0%
!! TATGAAAATTGGTATGATAA+CGG + chr2.1:4622461-4622480 None:intergenic 20.0%
!!! AGGATTTTAACATTGTTATT+AGG - chr2.1:4622609-4622628 MS.gene057021:intron 20.0%
!!! GTTTATGATATTCAATGTTT+TGG - chr2.1:4622307-4622326 MS.gene057021:CDS 20.0%
! ATGAAAATTGGTATGATAAC+GGG + chr2.1:4622460-4622479 None:intergenic 25.0%
!!! ATGCTTCATTTTAATTTTGG+AGG - chr2.1:4622900-4622919 MS.gene057021:intron 25.0%
AGATACTGATAAGGAGAATA+TGG + chr2.1:4622336-4622355 None:intergenic 30.0%
GACTAAAACAGATACTGATA+AGG + chr2.1:4622345-4622364 None:intergenic 30.0%
! TGAAAATTGGTATGATAACG+GGG + chr2.1:4622459-4622478 None:intergenic 30.0%
AACTAAAATGCCATAGCAGA+TGG - chr2.1:4622797-4622816 MS.gene057021:intron 35.0%
ATCTTTATGTTGCGTGGTTT+GGG - chr2.1:4622371-4622390 MS.gene057021:intron 35.0%
CACGCAACATAAAGATAACA+GGG + chr2.1:4622367-4622386 None:intergenic 35.0%
TAGTTGTTACAGCATCAGTT+AGG + chr2.1:4622782-4622801 None:intergenic 35.0%
TATCTTTATGTTGCGTGGTT+TGG - chr2.1:4622370-4622389 MS.gene057021:intron 35.0%
! AACAGCATTTTCTGACAATG+TGG - chr2.1:4622263-4622282 MS.gene057021:CDS 35.0%
! AGCTGTTTGGTATCATTTTC+TGG + chr2.1:4622824-4622843 None:intergenic 35.0%
! GCTGTTCTTTACGATTGTTT+TGG - chr2.1:4622422-4622441 MS.gene057021:intron 35.0%
! GCTGTTTGGTATCATTTTCT+GGG + chr2.1:4622823-4622842 None:intergenic 35.0%
! TGCATTGTTCCTTTTTGCTT+GGG - chr2.1:4622394-4622413 MS.gene057021:intron 35.0%
!! ATGATAACGGGGAACAATAA+TGG + chr2.1:4622448-4622467 None:intergenic 35.0%
!! CCAAACAGCTCTAAAAGATT+TGG - chr2.1:4622834-4622853 MS.gene057021:intron 35.0%
!! TTAATTTTGGAGGCTCTTGA+AGG - chr2.1:4622910-4622929 MS.gene057021:intron 35.0%
!!! CCAAATCTTTTAGAGCTGTT+TGG + chr2.1:4622837-4622856 None:intergenic 35.0%
AACAGCCAAAAGTTCATCAC+TGG + chr2.1:4622222-4622241 None:intergenic 40.0%
ATCACCATTGAGTTTGGAGA+AGG - chr2.1:4623036-4623055 MS.gene057021:intron 40.0%
CCACGCAACATAAAGATAAC+AGG + chr2.1:4622368-4622387 None:intergenic 40.0%
CCTGTTATCTTTATGTTGCG+TGG - chr2.1:4622365-4622384 MS.gene057021:intron 40.0%
TGCTACTAAAGAGTCGAAGA+TGG - chr2.1:4623062-4623081 MS.gene057021:CDS 40.0%
TTGTTACAGCATCAGTTAGG+CGG + chr2.1:4622779-4622798 None:intergenic 40.0%
! ACCTTCCAGTGATGAACTTT+TGG - chr2.1:4622214-4622233 MS.gene057021:CDS 40.0%
! GTGCATTGTTCCTTTTTGCT+TGG - chr2.1:4622393-4622412 MS.gene057021:intron 40.0%
! TTGTTCCTTTTTGCTTGGGT+TGG - chr2.1:4622398-4622417 MS.gene057021:intron 40.0%
AGAGCCTTCTCCAAACTCAA+TGG + chr2.1:4623043-4623062 None:intergenic 45.0%
AGGAGGTGATTCAAGCAACA+AGG - chr2.1:4622930-4622949 MS.gene057021:intron 45.0%
CAAGCAAACCGTTGGGAAAA+AGG - chr2.1:4622983-4623002 MS.gene057021:intron 45.0%
GCCAAAAGTTCATCACTGGA+AGG + chr2.1:4622218-4622237 None:intergenic 45.0%
GGAGGTGATTCAAGCAACAA+GGG - chr2.1:4622931-4622950 MS.gene057021:intron 45.0%
TGATTCAAGCAACAAGGGAG+AGG - chr2.1:4622936-4622955 MS.gene057021:intron 45.0%
TGTGCGATCACCATTGAGTT+TGG - chr2.1:4623030-4623049 MS.gene057021:intron 45.0%
! ATTTTCTGGGCCATCTGCTA+TGG + chr2.1:4622810-4622829 None:intergenic 45.0%
!! ATTTTGGAGGCTCTTGAAGG+AGG - chr2.1:4622913-4622932 MS.gene057021:intron 45.0%
!!! TCAACTGGCCTTTTTCCCAA+CGG + chr2.1:4622994-4623013 None:intergenic 45.0%
ACTCCAGCAAGCAAACCGTT+GGG - chr2.1:4622976-4622995 MS.gene057021:intron 50.0%
ATCCCTTCGACACTCTCAAC+TGG + chr2.1:4623009-4623028 None:intergenic 50.0%
CAGCACCAACCCAAGCAAAA+AGG + chr2.1:4622406-4622425 None:intergenic 50.0%
TACTCCAGCAAGCAAACCGT+TGG - chr2.1:4622975-4622994 MS.gene057021:intron 50.0%
! TTTCCCAACGGTTTGCTTGC+TGG + chr2.1:4622982-4623001 None:intergenic 50.0%
!! AGGCCAGTTGAGAGTGTCGA+AGG - chr2.1:4623003-4623022 MS.gene057021:intron 55.0%
!! GGCCAGTTGAGAGTGTCGAA+GGG - chr2.1:4623004-4623023 MS.gene057021:intron 55.0%


Chromosome Type Strat End Strand Name
chr2.1 gene 4622180 4623107 4622180 ID=MS.gene057021
chr2.1 mRNA 4622180 4623107 4622180 ID=MS.gene057021.t1;Parent=MS.gene057021
chr2.1 exon 4623042 4623107 4623042 ID=MS.gene057021.t1.exon1;Parent=MS.gene057021.t1
chr2.1 CDS 4623042 4623107 4623042 ID=cds.MS.gene057021.t1;Parent=MS.gene057021.t1
chr2.1 exon 4622180 4622365 4622180 ID=MS.gene057021.t1.exon2;Parent=MS.gene057021.t1
chr2.1 CDS 4622180 4622365 4622180 ID=cds.MS.gene057021.t1;Parent=MS.gene057021.t1
Gene Sequence

>MS.gene057021

ATGCAGCTAAAGGAAGAGATGTGTTCAGTGAAAAACCTTCCAGTGATGAACTTTTGGCTGTTTCAAGTATTATAAAATCATTTAACAGCATTTTCTGACAATGTGGTGAGCTATCTTTATTTGACCTGTTTATGATATTCAATGTTTTGGCTGCCATATTCTCCTTATCAGTATCTGTTTTAGTCCCTGTTATCTTTATGTTGCGTGGTTTGGGTGCATTGTTCCTTTTTGCTTGGGTTGGTGCTGTTCTTTACGATTGTTTTGGCCATTATTGTTCCCCGTTATCATACCAATTTTCATAGCAATTGATTTAAATTGTTGACTATTTTCTTATTATATTAGTGTGATTCAAGTTAAGTTGCACATGTTTTTTCTTATCAAGAAAACAAACCTTTTTTAAGAAATTTTTTAACATTGTTTTATTTTTGAAGGATTTTAACATTGTTATTAGGTTAATTTATCGTAATTTAAATTCTAATTGACTATGAAAGTAAATATAGTTTGGTTGACTATATTAAGTAACATAAGCTGTTAAAAGACGTGCTATTCAAACTACTACCATCTCACTTTAATTCTTAATTCATCACACAGCTTGACCGCCTAACTGATGCTGTAACAACTAAAATGCCATAGCAGATGGCCCAGAAAATGATACCAAACAGCTCTAAAAGATTTGGACTAACTGTTATGCTTCATTTTAATTTACTCAAAGAAACTTTTATGCTTCATTTTAATTTTGGAGGCTCTTGAAGGAGGTGATTCAAGCAACAAGGGAGAGGCTGTTGCAACAGCTGATACTCCAGCAAGCAAACCGTTGGGAAAAAGGCCAGTTGAGAGTGTCGAAGGGATGTGTGCGATCACCATTGAGTTTGGAGAAGGCTCTGCTACTAAAGAGTCGAAGATGGTTTGCGTCAAGATGAAGATTTGA

Protein sequence

>MS.gene057021.t1

MQLKEEMCSVKNLPVMNFWLFQALEGGDSSNKGEAVATADTPASKPLGKRPVESVEGMCAITIEFGEGSATKESKMVCVKMKI