Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene39131.t1 | XP_024626467.1 | 98.3 | 121 | 2 | 0 | 1 | 121 | 1 | 121 | 3.80E-59 | 237.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene39131.t1 | O80366 | 77.9 | 122 | 26 | 1 | 1 | 121 | 1 | 122 | 1.2e-45 | 183.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene39131.t1 | A0A072U1M0 | 98.3 | 121 | 2 | 0 | 1 | 121 | 1 | 121 | 2.8e-59 | 237.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene39131.t1 | TR | Others |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050838 | MS.gene39131 | 0.800936 | 1.14E-48 | -1.69E-46 |
| MS.gene051687 | MS.gene39131 | 0.809789 | 1.59E-50 | -1.69E-46 |
| MS.gene056522 | MS.gene39131 | -0.800263 | 1.56E-48 | -1.69E-46 |
| MS.gene059171 | MS.gene39131 | 0.810647 | 1.04E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene39131.t1 | MTR_7g490310 | 98.347 | 121 | 2 | 0 | 1 | 121 | 1 | 121 | 1.91e-86 | 251 |
| MS.gene39131.t1 | MTR_8g038620 | 75.207 | 121 | 29 | 1 | 1 | 121 | 1 | 120 | 3.48e-59 | 180 |
| MS.gene39131.t1 | MTR_3g015490 | 72.897 | 107 | 29 | 0 | 7 | 113 | 2 | 108 | 1.51e-51 | 160 |
| MS.gene39131.t1 | MTR_6g007460 | 49.383 | 162 | 33 | 2 | 1 | 122 | 3 | 155 | 4.45e-43 | 140 |
| MS.gene39131.t1 | MTR_5g036480 | 61.404 | 114 | 39 | 2 | 8 | 121 | 15 | 123 | 1.79e-42 | 140 |
| MS.gene39131.t1 | MTR_1g049100 | 63.158 | 114 | 35 | 2 | 8 | 121 | 7 | 113 | 1.01e-41 | 136 |
| MS.gene39131.t1 | MTR_3g093860 | 58.929 | 112 | 39 | 2 | 10 | 121 | 19 | 123 | 2.97e-40 | 131 |
| MS.gene39131.t1 | MTR_3g078613 | 56.522 | 115 | 41 | 2 | 4 | 118 | 18 | 123 | 1.05e-38 | 129 |
| MS.gene39131.t1 | MTR_4g106590 | 57.983 | 119 | 40 | 3 | 4 | 121 | 21 | 130 | 1.68e-37 | 126 |
| MS.gene39131.t1 | MTR_3g088630 | 54.918 | 122 | 45 | 3 | 6 | 118 | 19 | 139 | 7.00e-37 | 125 |
| MS.gene39131.t1 | MTR_3g078613 | 56.034 | 116 | 41 | 3 | 4 | 118 | 18 | 124 | 7.63e-37 | 124 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene39131.t1 | AT3G57040 | 77.869 | 122 | 26 | 1 | 1 | 121 | 1 | 122 | 1.12e-63 | 194 |
| MS.gene39131.t1 | AT3G57040 | 77.869 | 122 | 26 | 1 | 1 | 121 | 1 | 122 | 1.12e-63 | 194 |
| MS.gene39131.t1 | AT2G41310 | 72.727 | 121 | 32 | 1 | 1 | 121 | 1 | 120 | 2.00e-59 | 182 |
| MS.gene39131.t1 | AT3G56380 | 55.932 | 118 | 44 | 1 | 4 | 121 | 15 | 124 | 3.68e-43 | 139 |
| MS.gene39131.t1 | AT2G40670 | 56.034 | 116 | 45 | 2 | 6 | 121 | 26 | 135 | 1.29e-41 | 135 |
| MS.gene39131.t1 | AT3G56380 | 55.462 | 119 | 44 | 2 | 4 | 121 | 15 | 125 | 2.36e-41 | 134 |
| MS.gene39131.t1 | AT3G48100 | 55.833 | 120 | 45 | 2 | 2 | 121 | 18 | 129 | 2.21e-40 | 133 |
| MS.gene39131.t1 | AT1G59940 | 58.772 | 114 | 38 | 2 | 8 | 121 | 32 | 136 | 2.61e-40 | 134 |
| MS.gene39131.t1 | AT2G40670 | 55.556 | 117 | 45 | 3 | 6 | 121 | 26 | 136 | 9.29e-40 | 130 |
| MS.gene39131.t1 | AT1G10470 | 59.649 | 114 | 37 | 2 | 8 | 121 | 19 | 123 | 1.10e-39 | 133 |
| MS.gene39131.t1 | AT1G74890 | 57.895 | 114 | 39 | 2 | 8 | 121 | 17 | 121 | 1.20e-39 | 131 |
| MS.gene39131.t1 | AT1G10470 | 59.649 | 114 | 37 | 2 | 8 | 121 | 33 | 137 | 1.34e-39 | 133 |
| MS.gene39131.t1 | AT1G19050 | 56.140 | 114 | 41 | 1 | 8 | 121 | 23 | 127 | 1.56e-39 | 131 |
| MS.gene39131.t1 | AT1G59940 | 58.772 | 114 | 38 | 2 | 8 | 121 | 124 | 228 | 5.71e-39 | 133 |
| MS.gene39131.t1 | AT5G62920 | 55.752 | 113 | 41 | 1 | 9 | 121 | 25 | 128 | 5.35e-38 | 127 |
Find 21 sgRNAs with CRISPR-Local
Find 84 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGAAATACAGATGGTGTATT+TGG | 0.194626 | 7.4:-18523989 | None:intergenic |
| AGAGTCACAGTTTCATGTTT+TGG | 0.318671 | 7.4:+18523544 | MS.gene39131:CDS |
| GCAGTTACTACAGTTGATTC+TGG | 0.344676 | 7.4:+18523917 | MS.gene39131:intron |
| GAAATACAGATGGTGTATTT+GGG | 0.355026 | 7.4:-18523988 | None:intergenic |
| ATGTGCCTTCAAGAATTAAT+AGG | 0.368999 | 7.4:+18525041 | MS.gene39131:CDS |
| AACTATACCTGATGATTGTT+AGG | 0.391930 | 7.4:-18524010 | None:intergenic |
| TACTGTATGCCTGGCATGAC+AGG | 0.404554 | 7.4:+18524198 | MS.gene39131:CDS |
| TATTACCTATTAATTCTTGA+AGG | 0.405802 | 7.4:-18525046 | None:intergenic |
| CTCAAAACATCTTCATATCA+AGG | 0.455916 | 7.4:+18523611 | MS.gene39131:CDS |
| TGTATTTCCTAACAATCATC+AGG | 0.478134 | 7.4:+18524003 | MS.gene39131:CDS |
| AAATCATAGCCTGTCATGCC+AGG | 0.488050 | 7.4:-18524207 | None:intergenic |
| TACAGTTGATTCTGGTAGCA+AGG | 0.501635 | 7.4:+18523925 | MS.gene39131:CDS |
| TTGATGACAGCATCATAGAT+AGG | 0.505605 | 7.4:+18523570 | MS.gene39131:CDS |
| ATAACAGATTACTGTATGCC+TGG | 0.512220 | 7.4:+18524189 | MS.gene39131:CDS |
| TACAGATGGTGTATTTGGGT+TGG | 0.527443 | 7.4:-18523984 | None:intergenic |
| TGATTTGCTTAAGAAAATCA+AGG | 0.528385 | 7.4:+18524224 | MS.gene39131:CDS |
| TCAGATGACATAATCACCAC+TGG | 0.584254 | 7.4:-18525016 | None:intergenic |
| TTAGTTGGAGAAAATGGGAA+TGG | 0.609092 | 7.4:+18523517 | None:intergenic |
| TAGATAGGAAGCTTATTGAG+AGG | 0.630074 | 7.4:+18523585 | MS.gene39131:CDS |
| ATCATTGAGAAACATACCAG+TGG | 0.696105 | 7.4:+18525000 | MS.gene39131:CDS |
| TGATTGTTAGGAAATACAGA+TGG | 0.757940 | 7.4:-18523998 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTAAACAATATGTCTTTAAA+TGG | + | chr7.4:18524632-18524651 | MS.gene39131:intron | 15.0% |
| !!! | AGATCAAAAAAATTACTTTT+TGG | + | chr7.4:18524502-18524521 | MS.gene39131:intron | 15.0% |
| !!! | TATGTTAATGTTTTTCTTTT+TGG | + | chr7.4:18523780-18523799 | MS.gene39131:intron | 15.0% |
| !! | ACAACATTATCATAGTATAA+TGG | - | chr7.4:18523709-18523728 | None:intergenic | 20.0% |
| !! | ACTATAGATTCATTCTAATT+TGG | + | chr7.4:18524694-18524713 | MS.gene39131:intron | 20.0% |
| !! | TATAATTAAAATGACCAAAG+TGG | - | chr7.4:18525110-18525129 | None:intergenic | 20.0% |
| !! | TATTACCTATTAATTCTTGA+AGG | - | chr7.4:18525049-18525068 | None:intergenic | 20.0% |
| !! | TCCAAAAAAAAACATTAACA+TGG | - | chr7.4:18523751-18523770 | None:intergenic | 20.0% |
| !! | TTAAAGTGGACAATAAATAT+TGG | - | chr7.4:18524291-18524310 | None:intergenic | 20.0% |
| !! | TTGAGAACATAAAATTTAGT+AGG | + | chr7.4:18524659-18524678 | MS.gene39131:intron | 20.0% |
| !!! | AAAAGATTTTGTTTCCAAAA+AGG | + | chr7.4:18524837-18524856 | MS.gene39131:intron | 20.0% |
| !!! | AAAGATTTTGTTTCCAAAAA+GGG | + | chr7.4:18524838-18524857 | MS.gene39131:intron | 20.0% |
| !!! | ACATGATCAATAATTGTTTT+AGG | - | chr7.4:18524433-18524452 | None:intergenic | 20.0% |
| !!! | ACCATGTTAATGTTTTTTTT+TGG | + | chr7.4:18523747-18523766 | MS.gene39131:intron | 20.0% |
| !!! | TAATGTTAGATTGTTTTTTC+TGG | + | chr7.4:18524110-18524129 | MS.gene39131:intron | 20.0% |
| !!! | TTATTTCTATTTTTCCACTT+TGG | + | chr7.4:18525093-18525112 | MS.gene39131:intron | 20.0% |
| ! | CTATAAAACACAAAAATCCA+AGG | - | chr7.4:18524148-18524167 | None:intergenic | 25.0% |
| ! | TCATCAGATTATGCTATATT+TGG | - | chr7.4:18523650-18523669 | None:intergenic | 25.0% |
| ! | TGAATGAATGAATGAATGAA+TGG | + | chr7.4:18523871-18523890 | MS.gene39131:intron | 25.0% |
| ! | TTAAAAGATATAGATGAGTC+AGG | - | chr7.4:18524268-18524287 | None:intergenic | 25.0% |
| ! | TTGATATAAGTCAAATCGAT+CGG | + | chr7.4:18524385-18524404 | MS.gene39131:intron | 25.0% |
| !! | ATGGATTATGTGTTTAAGAA+AGG | - | chr7.4:18523690-18523709 | None:intergenic | 25.0% |
| !! | CTTGGATTTTTGTGTTTTAT+AGG | + | chr7.4:18524146-18524165 | MS.gene39131:intron | 25.0% |
| !! | TTTAACCATCCAGTTTTTAA+AGG | + | chr7.4:18524525-18524544 | MS.gene39131:intron | 25.0% |
| !! | TTTTTGTGTTTTATAGGAAG+TGG | + | chr7.4:18524152-18524171 | MS.gene39131:intron | 25.0% |
| !!! | CATAATCTGATGAACTTTTT+TGG | + | chr7.4:18523657-18523676 | MS.gene39131:intron | 25.0% |
| !!! | TGATTTGCTTAAGAAAATCA+AGG | + | chr7.4:18524224-18524243 | MS.gene39131:CDS | 25.0% |
| !!! | TTTTTTGATCTCAGACAAAA+GGG | - | chr7.4:18524493-18524512 | None:intergenic | 25.0% |
| !!! | TTTTTTTGATCTCAGACAAA+AGG | - | chr7.4:18524494-18524513 | None:intergenic | 25.0% |
| AACTACGTTGTTTGAGAAAA+GGG | - | chr7.4:18524343-18524362 | None:intergenic | 30.0% | |
| AACTATACCTGATGATTGTT+AGG | - | chr7.4:18524013-18524032 | None:intergenic | 30.0% | |
| AAGAAAATCAAGGTAAGCTT+TGG | + | chr7.4:18524234-18524253 | MS.gene39131:intron | 30.0% | |
| AAGATTCTCTCTGATATTAC+AGG | + | chr7.4:18524970-18524989 | MS.gene39131:intron | 30.0% | |
| ACAACGTAGTTAGATTCAAA+TGG | + | chr7.4:18524352-18524371 | MS.gene39131:intron | 30.0% | |
| ATGTGCCTTCAAGAATTAAT+AGG | + | chr7.4:18525041-18525060 | MS.gene39131:CDS | 30.0% | |
| ATTGCTGAAAGTTATCAACT+TGG | + | chr7.4:18524726-18524745 | MS.gene39131:intron | 30.0% | |
| CTCAAAACATCTTCATATCA+AGG | + | chr7.4:18523611-18523630 | MS.gene39131:CDS | 30.0% | |
| GAAATACAGATGGTGTATTT+GGG | - | chr7.4:18523991-18524010 | None:intergenic | 30.0% | |
| GAGAGAATCTTATAAGTCTT+TGG | - | chr7.4:18524961-18524980 | None:intergenic | 30.0% | |
| TAACTACGTTGTTTGAGAAA+AGG | - | chr7.4:18524344-18524363 | None:intergenic | 30.0% | |
| TGATTGTTAGGAAATACAGA+TGG | - | chr7.4:18524001-18524020 | None:intergenic | 30.0% | |
| TGTATTTCCTAACAATCATC+AGG | + | chr7.4:18524003-18524022 | MS.gene39131:CDS | 30.0% | |
| TTGAAAAAGTTACTGCATCA+AGG | - | chr7.4:18524085-18524104 | None:intergenic | 30.0% | |
| ! | CATATCAAGGTAACTTTTGA+GGG | + | chr7.4:18523624-18523643 | MS.gene39131:intron | 30.0% |
| ! | TCATATCAAGGTAACTTTTG+AGG | + | chr7.4:18523623-18523642 | MS.gene39131:intron | 30.0% |
| ! | TTTTCCACTGAAAACAAATC+AGG | + | chr7.4:18524800-18524819 | MS.gene39131:intron | 30.0% |
| !! | CTTTTCCTTTAAAAACTGGA+TGG | - | chr7.4:18524533-18524552 | None:intergenic | 30.0% |
| !! | GACTCTTTTCCTTTAAAAAC+TGG | - | chr7.4:18524537-18524556 | None:intergenic | 30.0% |
| !! | TTACAAAAGTATGCCCTTTT+TGG | - | chr7.4:18524854-18524873 | None:intergenic | 30.0% |
| !!! | TGGACTTTTTGTTTTCTCAA+TGG | + | chr7.4:18523895-18523914 | MS.gene39131:intron | 30.0% |
| AATCGAAATCGAATTCTGGT+TGG | - | chr7.4:18524608-18524627 | None:intergenic | 35.0% | |
| AGATATAGATGAGTCAGGAA+TGG | - | chr7.4:18524263-18524282 | None:intergenic | 35.0% | |
| ATAACAGATTACTGTATGCC+TGG | + | chr7.4:18524189-18524208 | MS.gene39131:CDS | 35.0% | |
| ATCATTGAGAAACATACCAG+TGG | + | chr7.4:18525000-18525019 | MS.gene39131:CDS | 35.0% | |
| GAGAAATCGAAATCGAATTC+TGG | - | chr7.4:18524612-18524631 | None:intergenic | 35.0% | |
| GCTAGAAAGAGTGATTAAAG+TGG | - | chr7.4:18524305-18524324 | None:intergenic | 35.0% | |
| GGAAATACAGATGGTGTATT+TGG | - | chr7.4:18523992-18524011 | None:intergenic | 35.0% | |
| TAGATAGGAAGCTTATTGAG+AGG | + | chr7.4:18523585-18523604 | MS.gene39131:CDS | 35.0% | |
| ! | AGAGTCACAGTTTCATGTTT+TGG | + | chr7.4:18523544-18523563 | MS.gene39131:CDS | 35.0% |
| ! | TCTTATAAGTCTTTGGTTCC+AGG | - | chr7.4:18524954-18524973 | None:intergenic | 35.0% |
| ! | TTGATGACAGCATCATAGAT+AGG | + | chr7.4:18523570-18523589 | MS.gene39131:CDS | 35.0% |
| ! | TTGTGTTTTATAGGAAGTGG+AGG | + | chr7.4:18524155-18524174 | MS.gene39131:intron | 35.0% |
| ! | TTTTAGGCATAGACCAAACT+CGG | - | chr7.4:18524417-18524436 | None:intergenic | 35.0% |
| !! | GGACTTGCTAATATGAAGTA+CGG | - | chr7.4:18524472-18524491 | None:intergenic | 35.0% |
| !! | TCTGGAATTCTGAAATTCCT+TGG | + | chr7.4:18524128-18524147 | MS.gene39131:intron | 35.0% |
| !! | TGGAAAACTGAAAAGCATTC+AGG | - | chr7.4:18524787-18524806 | None:intergenic | 35.0% |
| !!! | AGCAAGGCTTTAGAGTTTTT+GGG | + | chr7.4:18523941-18523960 | MS.gene39131:CDS | 35.0% |
| !!! | TAGCAAGGCTTTAGAGTTTT+TGG | + | chr7.4:18523940-18523959 | MS.gene39131:CDS | 35.0% |
| !!! | TGAACCTGATTTGTTTTCAG+TGG | - | chr7.4:18524807-18524826 | None:intergenic | 35.0% |
| GCATTCAGGTGAATTATTGC+TGG | - | chr7.4:18524773-18524792 | None:intergenic | 40.0% | |
| GGAAAAGAGTCTTTGCAATC+CGG | + | chr7.4:18524546-18524565 | MS.gene39131:intron | 40.0% | |
| TACAGATGGTGTATTTGGGT+TGG | - | chr7.4:18523987-18524006 | None:intergenic | 40.0% | |
| TCAGATGACATAATCACCAC+TGG | - | chr7.4:18525019-18525038 | None:intergenic | 40.0% | |
| TGTGCAACACTTATTCAACC+TGG | + | chr7.4:18524933-18524952 | MS.gene39131:intron | 40.0% | |
| ! | GCAGTTACTACAGTTGATTC+TGG | + | chr7.4:18523917-18523936 | MS.gene39131:intron | 40.0% |
| ! | TACAGTTGATTCTGGTAGCA+AGG | + | chr7.4:18523925-18523944 | MS.gene39131:CDS | 40.0% |
| AAATCATAGCCTGTCATGCC+AGG | - | chr7.4:18524210-18524229 | None:intergenic | 45.0% | |
| AGTCTTTGCAATCCGGAGTT+CGG | + | chr7.4:18524553-18524572 | MS.gene39131:intron | 45.0% | |
| CGATCGGAAATATCCGAGTT+TGG | + | chr7.4:18524401-18524420 | MS.gene39131:intron | 45.0% | |
| !!! | TAATGTTTATTTATTTATTT+TGG | + | chr7.4:18523816-18523835 | MS.gene39131:intron | 5.0% |
| CTTAATTCCCGACCGAACTC+CGG | - | chr7.4:18524568-18524587 | None:intergenic | 50.0% | |
| TACTGTATGCCTGGCATGAC+AGG | + | chr7.4:18524198-18524217 | MS.gene39131:CDS | 50.0% | |
| TTTGCAATCCGGAGTTCGGT+CGG | + | chr7.4:18524557-18524576 | MS.gene39131:intron | 50.0% | |
| TTGCAATCCGGAGTTCGGTC+GGG | + | chr7.4:18524558-18524577 | MS.gene39131:intron | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 18523530 | 18525146 | 18523530 | ID=MS.gene39131 |
| chr7.4 | mRNA | 18523530 | 18525146 | 18523530 | ID=MS.gene39131.t1;Parent=MS.gene39131 |
| chr7.4 | exon | 18523530 | 18523632 | 18523530 | ID=MS.gene39131.t1.exon1;Parent=MS.gene39131.t1 |
| chr7.4 | CDS | 18523530 | 18523632 | 18523530 | ID=cds.MS.gene39131.t1;Parent=MS.gene39131.t1 |
| chr7.4 | exon | 18523921 | 18524024 | 18523921 | ID=MS.gene39131.t1.exon2;Parent=MS.gene39131.t1 |
| chr7.4 | CDS | 18523921 | 18524024 | 18523921 | ID=cds.MS.gene39131.t1;Parent=MS.gene39131.t1 |
| chr7.4 | exon | 18524168 | 18524245 | 18524168 | ID=MS.gene39131.t1.exon3;Parent=MS.gene39131.t1 |
| chr7.4 | CDS | 18524168 | 18524245 | 18524168 | ID=cds.MS.gene39131.t1;Parent=MS.gene39131.t1 |
| chr7.4 | exon | 18524992 | 18525062 | 18524992 | ID=MS.gene39131.t1.exon4;Parent=MS.gene39131.t1 |
| chr7.4 | CDS | 18524992 | 18525062 | 18524992 | ID=cds.MS.gene39131.t1;Parent=MS.gene39131.t1 |
| chr7.4 | exon | 18525134 | 18525146 | 18525134 | ID=MS.gene39131.t1.exon5;Parent=MS.gene39131.t1 |
| chr7.4 | CDS | 18525134 | 18525146 | 18525134 | ID=cds.MS.gene39131.t1;Parent=MS.gene39131.t1 |
| Gene Sequence |
| Protein sequence |
