AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene051687


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene051687.t1 MTR_1g017950 99.487 195 1 0 1 195 1 195 4.05e-146 404
MS.gene051687.t1 MTR_4g106800 66.857 175 57 1 2 176 3 176 1.70e-87 255
MS.gene051687.t1 MTR_3g070050 58.757 177 69 2 1 175 1 175 3.02e-70 212
MS.gene051687.t1 MTR_8g031490 50.820 183 83 3 1 182 1 177 2.64e-61 189
MS.gene051687.t1 MTR_8g097300 54.802 177 68 4 1 175 1 167 9.79e-61 188
MS.gene051687.t1 MTR_7g076720 50.857 175 82 2 1 175 1 171 3.28e-60 187
MS.gene051687.t1 MTR_3g070050 53.731 134 58 2 44 175 2 133 3.89e-45 146
MS.gene051687.t1 MTR_3g070050 47.826 69 36 0 3 71 61 129 1.42e-18 78.6
MS.gene051687.t1 MTR_1g106100 58.559 111 40 2 59 168 20 125 6.39e-38 128
MS.gene051687.t1 MTR_3g070033 66.038 53 18 0 123 175 6 58 3.58e-18 75.5
MS.gene051687.t1 MTR_3g070033 46.939 49 26 0 23 71 6 54 6.90e-11 56.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene051687.t1 AT1G10200 85.246 183 27 0 1 183 1 183 2.62e-116 328
MS.gene051687.t1 AT1G10200 82.014 139 25 0 45 183 3 141 2.77e-82 241
MS.gene051687.t1 AT1G10200 44.444 72 40 0 3 74 61 132 2.24e-16 72.8
MS.gene051687.t1 AT2G39900 58.192 177 69 2 1 175 1 174 2.13e-69 210
MS.gene051687.t1 AT3G55770 55.307 179 70 2 1 175 1 173 2.52e-67 205
MS.gene051687.t1 AT3G55770 43.678 87 49 0 103 189 3 89 5.85e-16 73.2
MS.gene051687.t1 AT3G55770 55.307 179 70 2 1 175 1 173 2.52e-67 205
MS.gene051687.t1 AT3G55770 43.678 87 49 0 103 189 3 89 5.85e-16 73.2
MS.gene051687.t1 AT3G55770 55.307 179 70 2 1 175 1 173 2.52e-67 205
MS.gene051687.t1 AT3G55770 43.678 87 49 0 103 189 3 89 5.85e-16 73.2
MS.gene051687.t1 AT3G55770 55.307 179 70 2 1 175 1 173 2.52e-67 205
MS.gene051687.t1 AT3G55770 43.678 87 49 0 103 189 3 89 5.85e-16 73.2
MS.gene051687.t1 AT2G45800 50.000 186 86 2 1 183 1 182 3.75e-61 190
MS.gene051687.t1 AT1G01780 48.649 185 87 3 1 183 1 179 2.17e-60 187
MS.gene051687.t1 AT3G61230 47.917 192 93 3 2 190 3 190 8.55e-60 186
MS.gene051687.t1 AT3G61230 36.792 106 67 0 3 108 100 205 3.58e-17 76.6
MS.gene051687.t1 AT3G55770 46.479 213 70 3 1 175 1 207 1.60e-59 186
MS.gene051687.t1 AT3G55770 43.678 87 49 0 103 189 3 89 1.44e-15 72.8
MS.gene051687.t1 AT3G55770 48.201 139 61 3 1 135 1 132 2.16e-40 134
MS.gene051687.t1 AT3G55770 43.678 87 49 0 103 189 3 89 5.56e-17 74.3
MS.gene051687.t1 AT2G45800 45.455 154 76 3 34 184 4 152 4.96e-39 132
MS.gene051687.t1 AT2G45800 50.725 69 34 0 3 71 68 136 1.35e-16 74.7
MS.gene051687.t1 AT3G55770 48.462 130 57 2 1 126 1 124 1.92e-38 129
MS.gene051687.t1 AT3G55770 43.678 87 49 0 103 189 3 89 5.92e-17 73.9

Find 36 sgRNAs with CRISPR-Local

Find 83 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCATTATCTATATTTCTTTC+TGG 0.117591 1.3:-4832133 None:intergenic
TCCCTCATGGGCTATGTAAT+TGG 0.293058 1.3:-4832700 None:intergenic
AGCATTTGAAACAACTCTTA+TGG 0.319973 1.3:-4832650 None:intergenic
TTTATAAAGGTTACAGTGAA+TGG 0.352930 1.3:+4832618 MS.gene051687:intron
TTTGCTTGATCAGTTGAATA+TGG 0.353364 1.3:-4832743 None:intergenic
CTTGAGGCTTTAGCTGCTGC+AGG 0.425078 1.3:-4832258 None:intergenic
TATTCAACTGATCAAGCAAA+AGG 0.432443 1.3:+4832746 MS.gene051687:CDS
GCTAAAGCCTCAAGTATGTT+TGG 0.441559 1.3:+4832267 MS.gene051687:CDS
GACCAACTGTTCAAAAGAAC+TGG 0.444337 1.3:+4831971 MS.gene051687:CDS
ACACTAATTACCTTCTCTGT+TGG 0.465114 1.3:-4832333 None:intergenic
GGAAATTTGAGCCAGCTTGA+AGG 0.475377 1.3:+4832768 MS.gene051687:CDS
GGAACAAGGGACAAATGTTC+TGG 0.477611 1.3:+4832291 MS.gene051687:CDS
TCTCATGGTCACCTTCAAGC+TGG 0.480325 1.3:-4832779 None:intergenic
CAAGTATGTTTGGTGGAACA+AGG 0.495604 1.3:+4832277 MS.gene051687:CDS
TTGAAACAACTCTTATGGTA+AGG 0.498185 1.3:-4832645 None:intergenic
AGAAAGAAATATAGATAATG+AGG 0.518674 1.3:+4832135 MS.gene051687:CDS
CCTTGAGGGTTCCTTTGCAG+TGG 0.520760 1.3:-4831493 None:intergenic
GAGAGAGACATAGACCTTGA+GGG 0.525903 1.3:-4831507 None:intergenic
GACTAATAGTACACCCTCCA+TGG 0.536605 1.3:-4832674 None:intergenic
AGGAACAACTCAGAAGTGTA+TGG 0.560932 1.3:+4831391 MS.gene051687:CDS
ATTCAACTGATCAAGCAAAA+GGG 0.562604 1.3:+4832747 MS.gene051687:CDS
AGTTGTTTCAAATGCTGCCA+TGG 0.564010 1.3:+4832657 MS.gene051687:CDS
GACATCTGAAACAAGCTTTG+TGG 0.565667 1.3:-4831469 None:intergenic
TTGAACAGTTGGTCGAAGTG+TGG 0.580292 1.3:-4831962 None:intergenic
TTCAGATGTCACCACTGCAA+AGG 0.582786 1.3:+4831482 MS.gene051687:CDS
GCTTGATCAGTTGAATATGG+TGG 0.582930 1.3:-4832740 None:intergenic
AAAGCCTCAAGTATGTTTGG+TGG 0.587944 1.3:+4832270 MS.gene051687:CDS
TCCAATTACATAGCCCATGA+GGG 0.590710 1.3:+4832699 MS.gene051687:CDS
GTTTCAAATGCTGCCATGGA+GGG 0.597804 1.3:+4832661 MS.gene051687:CDS
TGTTCCACCAAACATACTTG+AGG 0.606764 1.3:-4832274 None:intergenic
AACAGTGTATCCAACAGAGA+AGG 0.613878 1.3:+4832323 MS.gene051687:CDS
TTCCAATTACATAGCCCATG+AGG 0.614856 1.3:+4832698 MS.gene051687:CDS
TCATGGGCTATGTAATTGGA+AGG 0.623562 1.3:-4832696 None:intergenic
AAGTATGTTTGGTGGAACAA+GGG 0.641097 1.3:+4832278 MS.gene051687:CDS
CCACTGCAAAGGAACCCTCA+AGG 0.664342 1.3:+4831493 MS.gene051687:CDS
TGTTTCAAATGCTGCCATGG+AGG 0.668776 1.3:+4832660 MS.gene051687:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AACTCAAAAATAATTTTGTT+CGG - chr1.3:4832483-4832502 None:intergenic 15.0%
!!! TCAATAATTTGCATTTATAA+AGG + chr1.3:4832605-4832624 MS.gene051687:intron 15.0%
!! AGAAAGAAATATAGATAATG+AGG + chr1.3:4832135-4832154 MS.gene051687:CDS 20.0%
!! GTTCATTAACATGAAAATTA+CGG - chr1.3:4831807-4831826 None:intergenic 20.0%
!! TCATTATCTATATTTCTTTC+TGG - chr1.3:4832136-4832155 None:intergenic 20.0%
!! TTGTAGCTTCAATAAAATTA+CGG - chr1.3:4831671-4831690 None:intergenic 20.0%
! AAACAGCTCAAAAAATCATA+TGG - chr1.3:4832228-4832247 None:intergenic 25.0%
! AATGAACTTCTTTCATCATT+TGG + chr1.3:4831820-4831839 MS.gene051687:intron 25.0%
! AGGTAATGAATTCTAAATCT+AGG + chr1.3:4832155-4832174 MS.gene051687:intron 25.0%
! AGGTAATTAGTGTATATGTT+TGG + chr1.3:4832343-4832362 MS.gene051687:intron 25.0%
! CATAGCTTAAGTAAAAACTA+TGG + chr1.3:4831693-4831712 MS.gene051687:intron 25.0%
! GAAAATTGTTACTGCATATT+TGG - chr1.3:4831746-4831765 None:intergenic 25.0%
! TTTATAAAGGTTACAGTGAA+TGG + chr1.3:4832618-4832637 MS.gene051687:intron 25.0%
!! AGTGTATATGTTTGGAATTA+CGG + chr1.3:4832351-4832370 MS.gene051687:intron 25.0%
!!! AAGTTTTGTATTCAACATCA+GGG - chr1.3:4832567-4832586 None:intergenic 25.0%
!!! ACTTTTGTTTTGCATACATA+GGG + chr1.3:4832092-4832111 MS.gene051687:intron 25.0%
!!! TAAGTTTTGTATTCAACATC+AGG - chr1.3:4832568-4832587 None:intergenic 25.0%
!!! TACTTTTGTTTTGCATACAT+AGG + chr1.3:4832091-4832110 MS.gene051687:intron 25.0%
!!! TTTTGAGTTTGTGATTAGTT+TGG + chr1.3:4832494-4832513 MS.gene051687:intron 25.0%
AAAAATGCTGGGAAAATCAA+TGG + chr1.3:4832801-4832820 MS.gene051687:CDS 30.0%
ACCAAAAAGAAATGTCTAAG+AGG - chr1.3:4831537-4831556 None:intergenic 30.0%
ATTCAACTGATCAAGCAAAA+GGG + chr1.3:4832747-4832766 MS.gene051687:CDS 30.0%
CATTAACATGAAAATTACGG+TGG - chr1.3:4831804-4831823 None:intergenic 30.0%
TATTCAACTGATCAAGCAAA+AGG + chr1.3:4832746-4832765 MS.gene051687:CDS 30.0%
TGAAAATGCTTATGAACAAC+TGG - chr1.3:4831774-4831793 None:intergenic 30.0%
TTACTGCATATTTGGATTCA+CGG - chr1.3:4831738-4831757 None:intergenic 30.0%
TTGAAACAACTCTTATGGTA+AGG - chr1.3:4832648-4832667 None:intergenic 30.0%
TTTGCTTGATCAGTTGAATA+TGG - chr1.3:4832746-4832765 None:intergenic 30.0%
! AAGCAACTACAATTCTTTTG+AGG + chr1.3:4831922-4831941 MS.gene051687:CDS 30.0%
! ACCTCTTAGACATTTCTTTT+TGG + chr1.3:4831533-4831552 MS.gene051687:intron 30.0%
! AGCAACTACAATTCTTTTGA+GGG + chr1.3:4831923-4831942 MS.gene051687:CDS 30.0%
! AGCATTTGAAACAACTCTTA+TGG - chr1.3:4832653-4832672 None:intergenic 30.0%
! TTCTTTCATCATTTGGCTTA+TGG + chr1.3:4831827-4831846 MS.gene051687:intron 30.0%
!! AAAAGCTTTGAAGGTAACAA+AGG + chr1.3:4832004-4832023 MS.gene051687:intron 30.0%
!!! CTTTCTGGTTTGACAATTTT+TGG - chr1.3:4832121-4832140 None:intergenic 30.0%
!!! TACCTTCAAAGCTTTTTTCA+AGG - chr1.3:4832000-4832019 None:intergenic 30.0%
!!! TGTAGCCTTTTCTAAAATCA+TGG + chr1.3:4832424-4832443 MS.gene051687:intron 30.0%
ACACTAATTACCTTCTCTGT+TGG - chr1.3:4832336-4832355 None:intergenic 35.0%
CTAACAGATCCAATACTAGA+AGG - chr1.3:4831583-4831602 None:intergenic 35.0%
! AAGTATGTTTGGTGGAACAA+GGG + chr1.3:4832278-4832297 MS.gene051687:CDS 35.0%
! AGCCTTGAAAAAAGCTTTGA+AGG + chr1.3:4831995-4832014 MS.gene051687:CDS 35.0%
! CTAGTTTAGCCTTCTAGTAT+TGG + chr1.3:4831571-4831590 MS.gene051687:intron 35.0%
! TACCAGTTCTTTTGAACAGT+TGG - chr1.3:4831976-4831995 None:intergenic 35.0%
! TTTCATCATTTGGCTTATGG+AGG + chr1.3:4831830-4831849 MS.gene051687:intron 35.0%
! TTTTCCCAGCATTTTTCTCA+TGG - chr1.3:4832797-4832816 None:intergenic 35.0%
!! CGGCTAGTTTAACAATATCA+CGG + chr1.3:4832371-4832390 MS.gene051687:intron 35.0%
!! GTCCACCATGATTTTAGAAA+AGG - chr1.3:4832432-4832451 None:intergenic 35.0%
!!! AGCCTTTTCTAAAATCATGG+TGG + chr1.3:4832427-4832446 MS.gene051687:intron 35.0%
AAAGCCTCAAGTATGTTTGG+TGG + chr1.3:4832270-4832289 MS.gene051687:CDS 40.0%
AACAGTGTATCCAACAGAGA+AGG + chr1.3:4832323-4832342 MS.gene051687:CDS 40.0%
GACCAACTGTTCAAAAGAAC+TGG + chr1.3:4831971-4831990 MS.gene051687:CDS 40.0%
GCTAAAGCCTCAAGTATGTT+TGG + chr1.3:4832267-4832286 MS.gene051687:CDS 40.0%
GCTTGATCAGTTGAATATGG+TGG - chr1.3:4832743-4832762 None:intergenic 40.0%
GTGACCATGAGAAAAATGCT+GGG + chr1.3:4832790-4832809 MS.gene051687:CDS 40.0%
GTTCGGATTTGTGATGAGTT+TGG - chr1.3:4832466-4832485 None:intergenic 40.0%
TCCAATTACATAGCCCATGA+GGG + chr1.3:4832699-4832718 MS.gene051687:CDS 40.0%
TGCAACTTTCGCCAAAATCA+CGG - chr1.3:4832538-4832557 None:intergenic 40.0%
TGTTCCACCAAACATACTTG+AGG - chr1.3:4832277-4832296 None:intergenic 40.0%
TTCCAATTACATAGCCCATG+AGG + chr1.3:4832698-4832717 MS.gene051687:CDS 40.0%
! AGGAACAACTCAGAAGTGTA+TGG + chr1.3:4831391-4831410 MS.gene051687:CDS 40.0%
! AGTTGTTTCAAATGCTGCCA+TGG + chr1.3:4832657-4832676 MS.gene051687:CDS 40.0%
! CAAGTATGTTTGGTGGAACA+AGG + chr1.3:4832277-4832296 MS.gene051687:CDS 40.0%
! GACATCTGAAACAAGCTTTG+TGG - chr1.3:4831472-4831491 None:intergenic 40.0%
! TCATGGGCTATGTAATTGGA+AGG - chr1.3:4832699-4832718 None:intergenic 40.0%
GACTAATAGTACACCCTCCA+TGG - chr1.3:4832677-4832696 None:intergenic 45.0%
GAGAGAGACATAGACCTTGA+GGG - chr1.3:4831510-4831529 None:intergenic 45.0%
GATGAGTTTGGCAGAATCTG+TGG - chr1.3:4832454-4832473 None:intergenic 45.0%
GGAAATTTGAGCCAGCTTGA+AGG + chr1.3:4832768-4832787 MS.gene051687:CDS 45.0%
GGAACAAGGGACAAATGTTC+TGG + chr1.3:4832291-4832310 MS.gene051687:CDS 45.0%
GGTGACCATGAGAAAAATGC+TGG + chr1.3:4832789-4832808 MS.gene051687:CDS 45.0%
GTTTCAAATGCTGCCATGGA+GGG + chr1.3:4832661-4832680 MS.gene051687:CDS 45.0%
TGAGAGAGACATAGACCTTG+AGG - chr1.3:4831511-4831530 None:intergenic 45.0%
TGTTTCAAATGCTGCCATGG+AGG + chr1.3:4832660-4832679 MS.gene051687:CDS 45.0%
TTCAGATGTCACCACTGCAA+AGG + chr1.3:4831482-4831501 MS.gene051687:CDS 45.0%
TTGAACAGTTGGTCGAAGTG+TGG - chr1.3:4831965-4831984 None:intergenic 45.0%
! GCAGTAGAGTTTTCCCTCAT+GGG - chr1.3:4832715-4832734 None:intergenic 45.0%
! TCATTGTGCGACCGTGATTT+TGG + chr1.3:4832524-4832543 MS.gene051687:intron 45.0%
! TCCCTCATGGGCTATGTAAT+TGG - chr1.3:4832703-4832722 None:intergenic 45.0%
! TGCAGTAGAGTTTTCCCTCA+TGG - chr1.3:4832716-4832735 None:intergenic 45.0%
TCTCATGGTCACCTTCAAGC+TGG - chr1.3:4832782-4832801 None:intergenic 50.0%
CCACTGCAAAGGAACCCTCA+AGG + chr1.3:4831493-4831512 MS.gene051687:CDS 55.0%
CCTTGAGGGTTCCTTTGCAG+TGG - chr1.3:4831496-4831515 None:intergenic 55.0%
! CTTGAGGCTTTAGCTGCTGC+AGG - chr1.3:4832261-4832280 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr1.3 gene 4831380 4832848 4831380 ID=MS.gene051687
chr1.3 mRNA 4831380 4832848 4831380 ID=MS.gene051687.t1;Parent=MS.gene051687
chr1.3 exon 4831380 4831514 4831380 ID=MS.gene051687.t1.exon1;Parent=MS.gene051687.t1
chr1.3 CDS 4831380 4831514 4831380 ID=cds.MS.gene051687.t1;Parent=MS.gene051687.t1
chr1.3 exon 4831920 4832016 4831920 ID=MS.gene051687.t1.exon2;Parent=MS.gene051687.t1
chr1.3 CDS 4831920 4832016 4831920 ID=cds.MS.gene051687.t1;Parent=MS.gene051687.t1
chr1.3 exon 4832113 4832156 4832113 ID=MS.gene051687.t1.exon3;Parent=MS.gene051687.t1
chr1.3 CDS 4832113 4832156 4832113 ID=cds.MS.gene051687.t1;Parent=MS.gene051687.t1
chr1.3 exon 4832255 4832344 4832255 ID=MS.gene051687.t1.exon4;Parent=MS.gene051687.t1
chr1.3 CDS 4832255 4832344 4832255 ID=cds.MS.gene051687.t1;Parent=MS.gene051687.t1
chr1.3 exon 4832627 4832848 4832627 ID=MS.gene051687.t1.exon5;Parent=MS.gene051687.t1
chr1.3 CDS 4832627 4832848 4832627 ID=cds.MS.gene051687.t1;Parent=MS.gene051687.t1
Gene Sequence

>MS.gene051687

ATGGCATTTGCAGGAACAACTCAGAAGTGTATGGCTTGTAGCAAAACAGTTTATCTTGTTGATAAGTTAACTGCTGATAATAGAATTTTCCACAAAGCTTGTTTCAGATGTCACCACTGCAAAGGAACCCTCAAGGTCTATGTCTCTCTCATAACCTCTTAGACATTTCTTTTTGGTCACATATATATAGTCTAGTTTAGCCTTCTAGTATTGGATCTGTTAGAATCATAATCATCAAATTTAGTACATGTTTCAATAGACAGTGAGTTTAGCGAAATGACAATAACACCGTAATTTTATTGAAGCTACAAAGCATAGCTTAAGTAAAAACTATGGTAGCACACAATGAAAATCGCCGTGAATCCAAATATGCAGTAACAATTTTCTTTAACCAGTTGTTCATAAGCATTTTCATCTCAGACCACCGTAATTTTCATGTTAATGAACTTCTTTCATCATTTGGCTTATGGAGGTATGTTACTACATTGAAATAATTTTCATATACAAGTTTGCTGATAGATTAACGTATATACAATTCAGCTAAGCAACTACAATTCTTTTGAGGGAGTTCTTTACTGCAGACCACACTTCGACCAACTGTTCAAAAGAACTGGTAGCCTTGAAAAAAGCTTTGAAGGTAACAAAGGATACATAACTGAATTTGTTAATTAAATGTGTCACAGTACTTTTTTCTTGTCAGTGTCTAAATTGTACTTTTGTTTTGCATACATAGGGACACCAAAAATTGTCAAACCAGAAAGAAATATAGATAATGAGGTAATGAATTCTAAATCTAGGTGTTTACTGTTTACAATTCACAGTAGTAAATTCATGTTTTCAGCATTCCATATGATTTTTTGAGCTGTTTTAATCAGAAACCTGCAGCAGCTAAAGCCTCAAGTATGTTTGGTGGAACAAGGGACAAATGTTCTGGTTGTCAGAAAACAGTGTATCCAACAGAGAAGGTAATTAGTGTATATGTTTGGAATTACGGCTAGTTTAACAATATCACGGTGACACTGTAATTTTGTTGTAATTCTAAAGTGTAGCCTTTTCTAAAATCATGGTGGACCACAGATTCTGCCAAACTCATCACAAATCCGAACAAAATTATTTTTGAGTTTGTGATTAGTTTGGCAAAATCTCATTGTGCGACCGTGATTTTGGCGAAAGTTGCATCTAAACCCTGATGTTGAATACAAAACTTATTGTTCTACTAATGAAATCAATAATTTGCATTTATAAAGGTTACAGTGAATGGAACTCCTTACCATAAGAGTTGTTTCAAATGCTGCCATGGAGGGTGTACTATTAGTCCTTCCAATTACATAGCCCATGAGGGAAAACTCTACTGCAAACACCACCATATTCAACTGATCAAGCAAAAGGGAAATTTGAGCCAGCTTGAAGGTGACCATGAGAAAAATGCTGGGAAAATCAATGGCGAAGAAGTTGCAGCTGAAACTTGA

Protein sequence

>MS.gene051687.t1

MAFAGTTQKCMACSKTVYLVDKLTADNRIFHKACFRCHHCKGTLKLSNYNSFEGVLYCRPHFDQLFKRTGSLEKSFEGTPKIVKPERNIDNEKPAAAKASSMFGGTRDKCSGCQKTVYPTEKVTVNGTPYHKSCFKCCHGGCTISPSNYIAHEGKLYCKHHHIQLIKQKGNLSQLEGDHEKNAGKINGEEVAAET