AlfalfaGEDB | MS.gene39866

M. sativa cultivar XinJiangDaYe / MS.gene39866

KEGG: K21989
GO: GO:0005575 GO:0016020 GO:0110165
PFAM: PF02714.18 PF13967.9 PF14703.9

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene39866.t1	XP_003616997.1	98.6	766	11	0	1	766	1	766	0.00E+00	1502.6

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene39866.t1	Q9LVE4	75.9	746	177	2	1	743	1	746	0.0e+00	1139.0

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene39866.t1	G7KCP6	98.6	766	11	0	1	766	1	766	0.0e+00	1502.6

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050894	MS.gene39866	0.809741	1.63E-50	-1.69E-46

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PPI:

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Gene1	Gene2	Type
MS.gene016796	MS.gene39866	PPI
MS.gene018069	MS.gene39866	PPI
MS.gene067383	MS.gene39866	PPI
MS.gene070123	MS.gene39866	PPI
MS.gene39866	MS.gene72873	PPI
MS.gene39866	MS.gene028031	PPI
MS.gene39866	MS.gene04704	PPI
MS.gene52745	MS.gene39866	PPI
MS.gene81560	MS.gene39866	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene39866.t1	MTR_5g086610	98.564	766	11	0	1	766	1	766	0.0	1563
MS.gene39866.t1	MTR_4g132570	71.879	761	207	3	1	755	1	760	0.0	1139
MS.gene39866.t1	MTR_7g094570	65.879	762	254	3	1	759	1	759	0.0	1047
MS.gene39866.t1	MTR_5g027510	62.483	717	267	1	1	715	1	717	0.0	952
MS.gene39866.t1	MTR_6g012870	32.578	706	446	11	10	711	9	688	5.71e-138	424
MS.gene39866.t1	MTR_7g011610	32.906	702	446	10	12	711	11	689	3.37e-132	411
MS.gene39866.t1	MTR_4g082340	31.623	721	453	14	2	715	5	692	9.34e-123	384
MS.gene39866.t1	MTR_4g082340	31.276	729	454	14	2	715	5	701	2.75e-121	381
MS.gene39866.t1	MTR_6g012870	33.166	597	375	9	10	605	9	582	1.39e-120	375
MS.gene39866.t1	MTR_4g082340	31.679	685	432	11	2	679	5	660	7.92e-120	376

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	bit score
MS.gene39866.t1	AT3G21620	75.871	746	177	2	1	743	1	746	1148
MS.gene39866.t1	AT1G11960	74.274	758	186	3	1	749	1	758	1146
MS.gene39866.t1	AT1G11960	74.274	758	186	3	1	749	1	758	1146
MS.gene39866.t1	AT4G22120	73.342	754	196	2	1	749	1	754	1137
MS.gene39866.t1	AT4G22120	73.342	754	196	2	1	749	1	754	1137
MS.gene39866.t1	AT4G22120	73.342	754	196	2	1	749	1	754	1137
MS.gene39866.t1	AT4G22120	73.342	754	196	2	1	749	1	754	1137
MS.gene39866.t1	AT4G22120	73.342	754	196	2	1	749	1	754	1137
MS.gene39866.t1	AT4G22120	73.342	754	196	2	1	749	1	754	1137
MS.gene39866.t1	AT4G15430	72.848	755	202	1	1	752	1	755	1135

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Find 160 sgRNAs with CRISPR-Local

Find 365 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGACCGTGAAGAAGCAA+TGG	0.595265	5.4:+68421133	MS.gene39866:CDS
AAAATGCTAGGAGTGAAATG+GGG	0.730707	5.4:-68417644	None:intergenic
AAACCCCTCAGATACCATTT+CGG	0.427688	5.4:-68416638	None:intergenic
AAACTAGAAATCCAACAATA+TGG	0.385358	5.4:+68419671	MS.gene39866:CDS
AAATACACCCTATCGTTTAT+AGG	0.291133	5.4:-68416614	None:intergenic
AAATCTTTCAAATTGAAGTT+TGG	0.330467	5.4:-68422131	None:intergenic
AACAATATGGTTGACTGAAT+GGG	0.447238	5.4:+68419684	MS.gene39866:CDS
AACCAAGAATATGAGAGTGC+TGG	0.536287	5.4:+68421696	MS.gene39866:CDS
AACCACATTTGAACGTTTCA+AGG	0.375878	5.4:-68417700	None:intergenic
AACTTGATCATACCGTGAAT+TGG	0.448801	5.4:-68418230	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAATACACCCTATCGTTTAT+AGG	-	chr5.4:68416617-68416636	None:intergenic	30.0%
AAATACTAATCCCTATGTGT+AGG	+	chr5.4:68420239-68420258	MS.gene39866:intron	30.0%
AACAATATGGTTGACTGAAT+GGG	+	chr5.4:68419684-68419703	MS.gene39866:CDS	30.0%
AACAGATTTGGAAGATGATT+TGG	-	chr5.4:68419058-68419077	None:intergenic	30.0%
AAGCAAAGGTTACAAATATG+AGG	-	chr5.4:68419404-68419423	None:intergenic	30.0%
AAGTAAGGACTAAACTTAGA+AGG	+	chr5.4:68417463-68417482	MS.gene39866:intron	30.0%
ATATTCCACTTGGATCAAAT+AGG	+	chr5.4:68417754-68417773	MS.gene39866:CDS	30.0%
ATGATGTATGGAAGTAGAAA+TGG	-	chr5.4:68421242-68421261	None:intergenic	30.0%
ATTGGTTAAAATCTACTCTC+TGG	-	chr5.4:68416900-68416919	None:intergenic	30.0%
CAATGCAAAATAACGATTCT+GGG	+	chr5.4:68418790-68418809	MS.gene39866:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr5.4	gene	68416521	68422346	68416521	ID=MS.gene39866
chr5.4	mRNA	68416521	68422346	68416521	ID=MS.gene39866.t1;Parent=MS.gene39866
chr5.4	exon	68416521	68416834	68416521	ID=MS.gene39866.t1.exon1;Parent=MS.gene39866.t1
chr5.4	CDS	68416521	68416834	68416521	ID=cds.MS.gene39866.t1;Parent=MS.gene39866.t1
chr5.4	exon	68417629	68417775	68417629	ID=MS.gene39866.t1.exon2;Parent=MS.gene39866.t1
chr5.4	CDS	68417629	68417775	68417629	ID=cds.MS.gene39866.t1;Parent=MS.gene39866.t1
chr5.4	exon	68418104	68418239	68418104	ID=MS.gene39866.t1.exon3;Parent=MS.gene39866.t1
chr5.4	CDS	68418104	68418239	68418104	ID=cds.MS.gene39866.t1;Parent=MS.gene39866.t1
chr5.4	exon	68418394	68418495	68418394	ID=MS.gene39866.t1.exon4;Parent=MS.gene39866.t1
chr5.4	CDS	68418394	68418495	68418394	ID=cds.MS.gene39866.t1;Parent=MS.gene39866.t1
chr5.4	exon	68418900	68419019	68418900	ID=MS.gene39866.t1.exon5;Parent=MS.gene39866.t1
chr5.4	CDS	68418900	68419019	68418900	ID=cds.MS.gene39866.t1;Parent=MS.gene39866.t1
chr5.4	exon	68419158	68419238	68419158	ID=MS.gene39866.t1.exon6;Parent=MS.gene39866.t1
chr5.4	CDS	68419158	68419238	68419158	ID=cds.MS.gene39866.t1;Parent=MS.gene39866.t1
chr5.4	exon	68419562	68419902	68419562	ID=MS.gene39866.t1.exon7;Parent=MS.gene39866.t1
chr5.4	CDS	68419562	68419902	68419562	ID=cds.MS.gene39866.t1;Parent=MS.gene39866.t1
chr5.4	exon	68420602	68420847	68420602	ID=MS.gene39866.t1.exon8;Parent=MS.gene39866.t1
chr5.4	CDS	68420602	68420847	68420602	ID=cds.MS.gene39866.t1;Parent=MS.gene39866.t1
chr5.4	exon	68420977	68421301	68420977	ID=MS.gene39866.t1.exon9;Parent=MS.gene39866.t1
chr5.4	CDS	68420977	68421301	68420977	ID=cds.MS.gene39866.t1;Parent=MS.gene39866.t1
chr5.4	exon	68421681	68421914	68421681	ID=MS.gene39866.t1.exon10;Parent=MS.gene39866.t1
chr5.4	CDS	68421681	68421914	68421681	ID=cds.MS.gene39866.t1;Parent=MS.gene39866.t1
chr5.4	exon	68422092	68422346	68422092	ID=MS.gene39866.t1.exon11;Parent=MS.gene39866.t1
chr5.4	CDS	68422092	68422346	68422092	ID=cds.MS.gene39866.t1;Parent=MS.gene39866.t1

Gene Sequence

>MS.gene39866

ATGGCTTCACTTGGCGATATAGGACTTGCAGCTGCAATCAACATCCTTACTGCATTTGCATTCTTACTGGCATTTGCCATACTTCGGATTCAACCTATAAACGATAGGGTGTATTTTCCGAAATGGTATCTGAGGGGTTTAAGGTCCAGTCCACTGCAAGGAGGGGCTTTTGTGAGCAAATTTGTCAATATAGACTTCAGGTCATACATAAGGTTCTTAAACTGGATGCCTGCTGCATTGCAAATGCCGGAACCCGAACTAATTGAACACGCAGGCTTGGATTCTGCTGTATACTTGAGGATCTACTTACTCGGGTATGCTTTAGTACACTTTGACTCCTCTAAAGTTTGTAGTTCTGAATCGGTTACTAGAAAATCCAGAGAGTAGATTTTAACCAATACATGATTTATTATTTATACACAAGTAATATCAATAGTTAATTTTTTTCAAGCTTAGTCCTTACTTAATTTTAAGTTTTTGGTCCCTATAAAATTGGGAACTTCTAAGTTTAGTCCTTACTTAATTTTAATAGGTTTTTTAGTCCCTACAAAAAAAATTTACTTGTAAGTTTAGTCCCTGTTAAAACAAAGTTTGTTTAATTATCCTTAAACTCTTAAATTTTTGAACAATTTTTTTGACACATGTTTAGAACACTATGAAAGGTTCATTTACTAAAATTTAGAATTTTTTAACATGAAACGAATTAAATATGAATTTTTTAAAACGACAAAAGTTAAAGGTAAAAGTAACAAAATCTGGACGTAGAAATCAAATCTTGCGATAATTTTTTGTGGAGAAACTTTTAGTAATGTTCTTAACAAGTTTGTAAAAAATTGTTCAAAAATTTAAGAGTTTAAGCATAAATTAAACAAATTTGAATTTAGTAAGGACTAATACTGAGTGCATAAATTTTTTGTAGGGACTAAAAGAATTATGAAAATTAAGTAAGGACTAAACTTAGAAGGTCCCAATTTTATAGGGACCAAAAACATATTTAACCTTTTTTTTTTATAATAGTTGATATTTCCTACGTTTTTCTAGTTTCAAGATTGTTTACTTGTGTCTTTTCTATTTACTAACTGCATAATCTTATTCAATTGAATTGCAGGCTGAAAATATTTGTCCCCATTTCACTCCTAGCATTTTCTGTTATGGTCCCTGTCAATTGGACCAATGACACCTTGAAACGTTCAAATGTGGTTTATAGCAGCATCGATAAGCTTTCAATTTCAAATATTCCACTTGGATCAAATAGGTACGTAATAAGCAAACTTTTCAATTGTTACCATTCTTTTTTTGCACCTTCTTTGCCATCTATAGTCTATCATTTTAGTATGCATGAACTTCAGATTTACATAATATCCTTGTTTATTTAAATGAATAAGAATAATGCATTTCAGCATTTGATGAGAATTTTACACCTCTAACTCTAAACAATTCTATGCAAACTTCTTAATCAATATCAAACAAATTAATACATCTTCTGGTTATTGGTTTTACGAGGACTTATTCCCTAACCGAAATATTTGTTAAGCTTTTCATATAAGTATGTTTATCTTCCAGTAACTCATTACTTATTGCAGATTTTGGACTCACTTGGTAATGGCTTATGCTTTTACCTTCTGGACATGCTATATCTTGAAAAGGGAGTATCAGATAGTTGCAGCAATGAGATTGTCTTTTCTTGCATCTGAAAGACGCCGTCCTGACCAATTCACGGTATGATCAAGTTTTATCACACTCACAGATGCAATATGCTGATAATCATAGTAGCACCGTTTCTATGATAAAGATTTATATTCTAAAGTGACTTTGTTCTTTCATTTTGTTCCCCCATGCTCCTTCTTCAAGCAATTACTGTGCATTTGTGCAGGTACTTGTTAGGAATGTACCGCCTGATGCTGATGAATCAGTCAGTGAGCTAGTTGAACATTTCTTTTTGGTGAACCATCCTGATCAATATCTAACTCATCAGGTTTGATCCTGTTTTCTGTAACTGCACATACCTAATTGCCTCTCTTCATCTGGAGCTTGTAATAAGAATAATAATGTTGTGTAACTTTCACTATTTAATTACACGTATCAAAACAATCAGTTTTACCTGTTATGACTTCAAATTCATATTCATTTTTACGTATGTACAAATTGATAGTAGTTCTTCTCTAGTATAAGAAGTAAATAATGTGATGTTTCTTAGAGAATTATATCTTGTTAACTGGTACTAAGATTCAGATCTTTGTAATGTTTTCCGATACCTTTGGGTAAAATTCAATGCAAAATAACGATTCTGGGATGAATTCTTATTGGCAGTTTGTTGTGTCTTATCCTACCAAGTTGTTTCATTGTGTTTTCCCTTACTCTTAAGTGATATTTTTGCAGGTTGTTTACGATGCAAAAAAACTCTCTAGTCTAGTTGCTAAGAAGAAGAAAAAACAGAATTGGCTTGACTACTATGAACTTAAATATTCTAGAAACGAATCCATAAGGCCAACTAAAAAGGTATGTCATCTCCAGTAATCCTGTCTCTATGAACTCCAAATCATCTTCCAAATCTGTTGCCATCTTTTTAAAGGTATTCTATTTCCCTTTCTTCCCTCTGGCACCATGTAATTCTAGTTGGTCACATTGTGCTTGCAGACTGGCTTTTTAGGTCTTTGTGGCAGTAAAGTGGATGCTATTGATTTTTATACTGCTGCAATTGAGAGACTATCAAGAGACGTAAGCAGAACTGCATGTGCATACTTTATATCATTTAAATATACAGCAATTATTATTGTACCACACTTGTATTATGAAAATCTTAATAAAGGGTGCATGAAAAAGATATATAGATCATATCAAAAGTGTTTTTTGTATGATGCCATACAAGAGAACTTGTGTCCTCATATTTGTAACCTTTGCTTATTTTCATCATTGCAGTGCATGCTTCTCCAAATTGGTTTTTTAGTTAAATATATTTCTTGTATGATAAGTTATTGAATGTTCATTACTTTTTCAAAAAAAAAGTATTTAATATTAGTTGATGTTTCGTTAAATTTCAGATAGAGCTGGAGAAAGATAAGGTGACAAAGAATCCTAAATCTATAATGCCAGCAGCCTTTGTTTCCTTCAAAACTCGTTGGGGTGCTGCAGTTTGTGCACAAACTCAACAAACTAGAAATCCAACAATATGGTTGACTGAATGGGCTCCAGAGCCCCGTGACATATACTGGGATAACATGGCTATACCATATGTTTCTCTCTCAATAAGGAGACTTGTAATTGGTGTTGCGTTCTTCTTTCTTACATTCTTTTTCATGATTCCCATTGCATTCGTACAGTCACTTGCTAATATAGAAGGCATTGAAAAAGCCGCACCATTCCTCAAGTCCATTATTGAAATGTAATGTTATCTTCTTTTTGCCTTTTATTACATTTTTCTCTTTATTGGTTGACATAGTAAACAAAATGTGTTGAAAAGTATGTGTTGAAGTTATGAGTTTTAGATAAAAAATAAAAATAAAAAAAAATAGAGAAATAATAATAGCTTGAACAACCCATCTGGGTTGGTCTCGTGGTGTTGACTTGGGACCTTACCTTAGAGTTTGCTCCTCTCAAGGTCTCAGGTTCGATTCTCCCTGGTGCTAAGTTTGGTGGGCTAGTTTAGTTTATTCAAAAAATAATAATAATAGCTTGAATACTATTTATAATAGTTTATAGTAACTTGATACAAAAGGCTAAATACTAATCCCTATGTGTAGGACTACATTGACTCAAACGTTAAATAAATAGAAATTTCAGGAAAGAAATCATATTATAATAGCTAATAATAAGTAGTCCTAAGAGATAATGTAAGATTATAAACTGATCTTAGAATGAACTAAGAATCTCTAATATAATATGATATTGTATGGAACATTTTGCTATTGTTATGATGAAAAGAATGCTATCTAGTCTGATTACATCACGGAATATGCTGGAAGTTTATGTCTTTCACTGGCAACTTTGATGAGAAAATAAAATATTGCAGATAACTCTTGTGACTCTTTGATAACTGTCTATCCATGGTAGATGTTAATGTTATGCATCATTTTTATTGCAGCGACGTCATCAAGTCTTTTATACAAGGTTTCCTTCCTGGGATTGCTTTGAAGCTATTCCTCATTTTTTTGCCAACAATATTGATGATCATGTCCAAGTTCGAAGGGTTTATAAGTCAGTCTTCTCTGGAAAGAAGATGCGCCTCAAGATATTACATCTTCCAGTTCATTAATGTGTTTCTGGGGAGCATAATTACCGGGACTGCATTCCAACAACTAGATAAATTCATTCATCAGTCTGCAAATGAGTAAGTGTTCCTCCTATGCATATATATATGACTGTATGTGTTTGTGTGTACGTACATGTGTGTGTGTGTGTGTGTTTGATTTACCTGTACATGATTTGTTCATAATGCAAAACATTTGTATGTATGCAGAATTCCAAAGACAATTGGTGTTTCGATTCCAATGAAAGCAACTTTCTTCATAACATACATAATGGTTGATGGATGGGCAGGATGTGCTGGTGAGATTTTAAGGTTGAAGCCTTTGATATTTTATCACTTAAAGAATTTCTTGTTGGTGAAGACTGAAAAAGACCGTGAAGAAGCAATGGATCCTGGGACTATTGGTTTCAATACAGGAGAACCTCAGATACAACTTTATTTCTTGCTTGGCCTTGTGTATGCTGTAGTCACACCATTTCTACTTCCATACATCATTGTCTTTTTTGGTTTGGCATATCTTGTCTACCGTCATCAGGTAAACATTAGCTCGTAGAACCTTCGCTGTTTTGATGCTTTTCATTACTGATTTTAGTATTTTACATGAGGGGTAACCTTGGCGCAACTGTAAGGTCACGGGTTCAAGTCCTGGAAACAGCCTCTTGTGTAAAAACAGGGTAAGGCTGCGTACAATACACCAAATGGTGGGACCCCTTCCCGGACCCTGCGTATGCGGGAGCTTCAGTGCACCGGGTTGCCCTTTTACATATTTACCATGAAAATGAGTGCTATGTTGTGTGTGTACAACTTTGGTTATTCAAACATGATTTTAGTCCATGAGCATATATTAAAATCGACATCCTTAAGTATTTTAGTGCATGTATTGTATGATATTAATGTCTGAAACTGACTGGCAGATTATAAACGTGTACAACCAAGAATATGAGAGTGCTGGAGCATTCTGGCCTGATGTCCATGGACGTATTGTATTTGCATTAGTTGTCTCACAACTTCTGTTAATGGGATTATTGAGTACAAAAGAAGCTGCTAATTCGACTCCATTACTCATCGCTCTCCCAGTTTTGACAATATGGTTTCATCGGTTTTGCAAGGGAAGCTACGAACCTGCTTTTACTACTCATCCATTACAGGTTTTTTTTTTTTTCTTTTTCTGATGTTGAAATTAGCAATATCTGTGTCTTCTTCTTTTAACTGCTTTGAGCAATCAAGTAAACAGAAAGCAATATTTTAGGGGACACCCTATGTTTTGAACCTGCTTCTTATAAAGCTTGAGTTAATTTTCTTTTTTTGTGGTGCATGAACTTCAGGAAGCAATGGTCAAAGACACATTGGAGCGTACTAAAGAGCCAAACTTCAATTTGAAAGATTTCCTCCATGATGCATATATCCATCCCGTTTTCAAAGATGACGGAGACACAGACAGTGATGTGATGAGTCAAGAGTGGAAGGAAGAGCCGGTAATTGTCCAAACAAAACGCCAGTCGCGGAAGAACACACCTGCGCCTAGCAAACATAGTGATGGCTCATTACAAGCCTCTATGCATGGCACTACTGATGTCTGA

Protein sequence

>MS.gene39866.t1

MASLGDIGLAAAINILTAFAFLLAFAILRIQPINDRVYFPKWYLRGLRSSPLQGGAFVSKFVNIDFRSYIRFLNWMPAALQMPEPELIEHAGLDSAVYLRIYLLGLKIFVPISLLAFSVMVPVNWTNDTLKRSNVVYSSIDKLSISNIPLGSNRFWTHLVMAYAFTFWTCYILKREYQIVAAMRLSFLASERRRPDQFTVLVRNVPPDADESVSELVEHFFLVNHPDQYLTHQVVYDAKKLSSLVAKKKKKQNWLDYYELKYSRNESIRPTKKTGFLGLCGSKVDAIDFYTAAIERLSRDIELEKDKVTKNPKSIMPAAFVSFKTRWGAAVCAQTQQTRNPTIWLTEWAPEPRDIYWDNMAIPYVSLSIRRLVIGVAFFFLTFFFMIPIAFVQSLANIEGIEKAAPFLKSIIEIDVIKSFIQGFLPGIALKLFLIFLPTILMIMSKFEGFISQSSLERRCASRYYIFQFINVFLGSIITGTAFQQLDKFIHQSANEIPKTIGVSIPMKATFFITYIMVDGWAGCAGEILRLKPLIFYHLKNFLLVKTEKDREEAMDPGTIGFNTGEPQIQLYFLLGLVYAVVTPFLLPYIIVFFGLAYLVYRHQIINVYNQEYESAGAFWPDVHGRIVFALVVSQLLLMGLLSTKEAANSTPLLIALPVLTIWFHRFCKGSYEPAFTTHPLQEAMVKDTLERTKEPNFNLKDFLHDAYIHPVFKDDGDTDSDVMSQEWKEEPVIVQTKRQSRKNTPAPSKHSDGSLQASMHGTTDV