AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene40310

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene40310.t1 MTR_3g110140 99.288 983 7 0 1 983 1 983 0.0 2006
MS.gene40310.t1 MTR_3g110090 99.172 845 7 0 139 983 5 849 0.0 1725
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene40310.t1 AT5G11560 66.633 983 325 2 3 983 1 982 0.0 1384

Find 211 sgRNAs with CRISPR-Local

Find 0 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATCCATCTCTTCTTGTAAT+TGG 0.112565 3.2:-87823729 MS.gene40310:CDS
GCACTTCCCTTTGCAACTTC+TGG 0.129619 3.2:-87825030 MS.gene40310:CDS
GGGTGTCTTCATGCCATTTC+TGG 0.139632 3.2:-87825317 MS.gene40310:CDS
ATGAATACTGCTTTGTATTC+AGG 0.210486 3.2:-87823392 MS.gene40310:CDS
CACAGGATAACAAAGATATT+TGG 0.229804 3.2:-87822198 MS.gene40310:intron
GCTTTGTATTCAGGGACTTA+TGG 0.251696 3.2:-87823383 MS.gene40310:CDS
CCGATTTCCCCACTGGCTTT+TGG 0.254400 3.2:+87822665 None:intergenic
CGCCATGGCCATGGCGATTA+GGG 0.259898 3.2:-87828210 None:intergenic
TTACTCAGATTGCTCCTTCT+AGG 0.262009 3.2:-87820667 MS.gene40310:CDS
TCTCAATATTTCCTCTTTGA+TGG 0.265891 3.2:+87824602 None:intergenic
TGTAGGTATTTAGTGAAAAC+TGG 0.270607 3.2:-87822456 MS.gene40310:intron
TTCGCCCCGTCTTCTGAGTA+TGG 0.273204 3.2:+87827707 None:intergenic
GCAGTTTGGAGGCATGTTCT+TGG 0.284280 3.2:-87827300 MS.gene40310:intron
TGCAGGCACCAACAGTATAT+TGG 0.286685 3.2:-87827751 MS.gene40310:intron
TCAATAGCTTCAGGAGTTCT+TGG 0.297756 3.2:+87823522 None:intergenic
GGTTTGGGAGTCTTCCCTTC+AGG 0.301565 3.2:-87826351 MS.gene40310:CDS
ATTTGTTACTTATGTATTAT+CGG 0.302509 3.2:-87820570 MS.gene40310:CDS
CACTTCCCTTTGCAACTTCT+GGG 0.302922 3.2:-87825029 MS.gene40310:CDS
TCGCCATGGCCATGGCGATT+AGG 0.319893 3.2:-87828211 None:intergenic
TACCTGACGGGCAGATGGTT+TGG 0.325931 3.2:-87826367 MS.gene40310:CDS
TGAGGTCTGAAACTTGCTTC+TGG 0.333539 3.2:+87824908 None:intergenic
ACCTCTAAGCAGCTTCTTAT+CGG 0.334847 3.2:-87822037 MS.gene40310:CDS
TCCGATAAGAAGCTGCTTAG+AGG 0.344251 3.2:+87822036 None:intergenic
ACCTGACGGGCAGATGGTTT+GGG 0.349641 3.2:-87826366 MS.gene40310:CDS
CATCAGGAGACTGTATAATA+TGG 0.362998 3.2:+87825145 None:intergenic
CCGGGGATGGACGCATAGTT+TGG 0.377141 3.2:-87823854 MS.gene40310:CDS
GCATAGACGGTGAAGTTCTC+TGG 0.380569 3.2:-87825295 MS.gene40310:CDS
GTTTGGGAGTCTTCCCTTCA+GGG 0.382494 3.2:-87826350 MS.gene40310:CDS
AGTAACAGTGCCTGCCAAAT+TGG 0.383144 3.2:-87820732 MS.gene40310:CDS
TGCTGTTCCGATTTCCCCAC+TGG 0.385316 3.2:+87822658 None:intergenic
GGAAAGATCTAGAAGAGAAA+TGG 0.389058 3.2:-87820544 MS.gene40310:CDS
AGGCTGCTGTATTGTCGATT+TGG 0.394539 3.2:+87824761 None:intergenic
CCTGATGTGATTTATGTAGC+TGG 0.397420 3.2:-87825129 MS.gene40310:CDS
CAGCTTCTTATCGGAACAAT+TGG 0.398831 3.2:-87822028 MS.gene40310:CDS
CCAAAAGCCAGTGGGGAAAT+CGG 0.402007 3.2:-87822665 MS.gene40310:CDS
ATCCATCTCTTCTTGTAATT+GGG 0.402204 3.2:-87823728 MS.gene40310:CDS
AACAGTCCTGGAAGCTTTGA+TGG 0.403066 3.2:+87824853 None:intergenic
GAACCTCTTCGAATGGCTTA+AGG 0.405315 3.2:-87824377 MS.gene40310:intron
TGATAAATGTTAAGCCCAAC+TGG 0.408225 3.2:+87823786 None:intergenic
CTGTAAAGGATGTTAATGAT+TGG 0.408833 3.2:-87824660 MS.gene40310:CDS
GAGGGATGTAGACTCCAATT+TGG 0.409191 3.2:+87820718 None:intergenic
GCAGGCACCAACAGTATATT+GGG 0.409945 3.2:-87827750 MS.gene40310:intron
TTCAGAATCTCAATAGCTTC+AGG 0.410731 3.2:+87823513 None:intergenic
TTAATTAAAGTTACAAATGA+AGG 0.411447 3.2:-87824802 MS.gene40310:CDS
CATGACCCACCACGGTTGCC+AGG 0.412068 3.2:-87822570 MS.gene40310:CDS
TGTTCAGTTGAATCAGTATA+AGG 0.412583 3.2:+87823591 None:intergenic
TTCAACAAGGAGAAATTGTC+TGG 0.414197 3.2:-87824492 MS.gene40310:CDS
ACTGATAATGGTGTTATTAG+AGG 0.418692 3.2:-87823453 MS.gene40310:CDS
TAACTTTAATTAAGAGAACT+TGG 0.420091 3.2:+87824812 None:intergenic
TATCGGAACAATTGGTGATC+AGG 0.426179 3.2:-87822020 MS.gene40310:intron
TCCCAAACCATCTGCCCGTC+AGG 0.426869 3.2:+87826365 None:intergenic
ATATTACCATCAAAGCTTCC+AGG 0.429030 3.2:-87824859 MS.gene40310:CDS
GATTGGTTAAAGAGAGGAAA+AGG 0.437129 3.2:+87828171 None:intergenic
TACTCGGTTGAGACTGATAA+TGG 0.442224 3.2:-87823465 MS.gene40310:CDS
GTAAAGAGAAGAAGCGAGAT+TGG 0.443952 3.2:+87828154 None:intergenic
AACACCAGAGGAGGCAACTT+TGG 0.447248 3.2:-87822636 MS.gene40310:CDS
CGAAGATCAAGTTGGTCTCA+TGG 0.448704 3.2:-87828132 MS.gene40310:CDS
TCATGGGTTCAGAGCATTGA+TGG 0.450368 3.2:-87824542 MS.gene40310:CDS
CAATAGCTTCAGGAGTTCTT+GGG 0.452351 3.2:+87823523 None:intergenic
GATATTTGGAAGTTTGTACT+TGG 0.455264 3.2:-87822184 MS.gene40310:CDS
GGCACCAATGATGTTGTTGA+TGG 0.456758 3.2:-87827279 MS.gene40310:CDS
AGCAGTTGAAGTCACTTCAA+TGG 0.456893 3.2:+87822069 None:intergenic
TGAATACTGCTTTGTATTCA+GGG 0.457304 3.2:-87823391 MS.gene40310:CDS
GTCATCACCCTTTCCTCTGA+TGG 0.458294 3.2:-87826413 MS.gene40310:CDS
GACCTCATCAAGGATTCATC+TGG 0.464150 3.2:-87824892 MS.gene40310:CDS
ACATCCCTCGTCTTTGCCTA+TGG 0.465598 3.2:-87820704 MS.gene40310:CDS
ATAACCAAAGTTGCCTCCTC+TGG 0.467336 3.2:+87822632 None:intergenic
TCTTCTTTCTCAGCTTGTGA+GGG 0.470188 3.2:+87821827 None:intergenic
AAAATGACTCGTGATCATAA+TGG 0.472672 3.2:-87823933 MS.gene40310:CDS
GTCAGAACTACCTGTGGAAA+AGG 0.473051 3.2:-87824428 MS.gene40310:CDS
TGAAGGTGAGTTGGTGGCGC+TGG 0.474644 3.2:-87824785 MS.gene40310:CDS
AGTGAATCGTAAGTCCTAGA+AGG 0.475966 3.2:+87820653 None:intergenic
TTCACTACCGATCATTTCTC+AGG 0.480117 3.2:-87821783 MS.gene40310:intron
TAAAATTCAGCTCTCTGTAA+AGG 0.484414 3.2:-87824674 MS.gene40310:CDS
ACACATTCGCTAAAAGTAGA+AGG 0.484558 3.2:-87820767 MS.gene40310:CDS
CAAAAGCTTACAGCATGAAC+AGG 0.485245 3.2:+87822545 None:intergenic
AGGTGATAAGTTTGTGGTGT+TGG 0.485329 3.2:-87824989 MS.gene40310:CDS
TTTGCTTTGCATACCGGGGA+TGG 0.485539 3.2:-87823867 MS.gene40310:CDS
TGCTTTGCTGAAGGAAAACT+TGG 0.490579 3.2:-87824632 MS.gene40310:CDS
GCTTAACATTTATCAGTGGC+AGG 0.492266 3.2:-87823778 MS.gene40310:CDS
TTCACCGTCTATGCCAGAAA+TGG 0.492390 3.2:+87825304 None:intergenic
AATCCCATCAACAACATCAT+TGG 0.493058 3.2:+87827275 None:intergenic
CTTATAGTACTTACAAGGGC+AGG 0.493287 3.2:-87823897 MS.gene40310:CDS
GGGGAATCATTATCAGTCTC+AGG 0.493338 3.2:-87825009 MS.gene40310:CDS
GTCAGAACAGATAGATCTCA+TGG 0.496977 3.2:-87824559 MS.gene40310:CDS
TGAAGTGACTTCAACTGCTA+AGG 0.499221 3.2:-87822065 MS.gene40310:CDS
AGTTGCTTATAGTACTTACA+AGG 0.500412 3.2:-87823902 MS.gene40310:CDS
AACCCTAATCGCCATGGCCA+TGG 0.501028 3.2:+87828208 None:intergenic
AAATAGCTATTCAAAAGCTT+AGG 0.505306 3.2:-87823977 MS.gene40310:CDS
TTCTTCTTTCTCAGCTTGTG+AGG 0.507689 3.2:+87821826 None:intergenic
TATACTTCCATCAGAGGAAA+GGG 0.508495 3.2:+87826406 None:intergenic
TCACCTTCCCAATATACTGT+TGG 0.508929 3.2:+87827743 None:intergenic
AGTTACAAATGAAGGTGAGT+TGG 0.509444 3.2:-87824794 MS.gene40310:CDS
TTGATTCCTTTGATCCCTGA+AGG 0.511496 3.2:+87826336 None:intergenic
TCGAGGTCTATGACCAGTCT+CGG 0.512629 3.2:-87822381 MS.gene40310:CDS
TGGTCGGTAATAAGAAGAGA+TGG 0.512641 3.2:+87822141 None:intergenic
CATCAAGGATTCATCTGGAC+AGG 0.512869 3.2:-87824887 MS.gene40310:CDS
ATGAAGTGAATGCAAAGAGC+GGG 0.513421 3.2:-87825077 MS.gene40310:CDS
CGCCCAATTACAAGAAGAGA+TGG 0.517391 3.2:+87823726 None:intergenic
GCTGACATACCGCCCGAGAC+TGG 0.517521 3.2:+87822368 None:intergenic
GATGGGATTGACATTGCCCT+TGG 0.518319 3.2:-87827261 MS.gene40310:CDS
ATCAAGTTGGTCTCATGGAT+TGG 0.519349 3.2:-87828127 MS.gene40310:intron
CAGATCCATCGCCATGGCCA+TGG 0.519382 3.2:-87828219 None:intergenic
CATGCCATTTCTGGCATAGA+CGG 0.521747 3.2:-87825308 MS.gene40310:CDS
GTGTTCCATACTCAGAAGAC+GGG 0.522168 3.2:-87827712 MS.gene40310:CDS
TTGTGGTGTTGGATGATGTA+AGG 0.522985 3.2:-87824978 MS.gene40310:CDS
GAGCCCTGAGATTAAAATAG+TGG 0.524090 3.2:+87822425 None:intergenic
GTTCAGAGCATTGATGGTAA+TGG 0.526198 3.2:-87824536 MS.gene40310:CDS
CCAGCTACATAAATCACATC+AGG 0.526361 3.2:+87825129 None:intergenic
GATGTAGACTCCAATTTGGC+AGG 0.527479 3.2:+87820722 None:intergenic
AGTCTCAGGTGATAAGTTTG+TGG 0.529071 3.2:-87824995 MS.gene40310:CDS
AGTACTTACAAGGGCAGGAA+AGG 0.530738 3.2:-87823892 MS.gene40310:CDS
TATATCATTGATACTGTGAC+TGG 0.532794 3.2:-87822608 MS.gene40310:CDS
ATCGGTAGTGAATCAGTAAG+AGG 0.536980 3.2:+87821794 None:intergenic
AGGATTGTGAACATCCAGTT+GGG 0.537160 3.2:-87823800 MS.gene40310:CDS
CCAAACTATGCGTCCATCCC+CGG 0.537428 3.2:+87823854 None:intergenic
TGAGGGATTGAGAGTCCGAC+GGG 0.540387 3.2:+87821844 None:intergenic
GTCTTCCCTTCAGGGATCAA+AGG 0.544092 3.2:-87826342 MS.gene40310:CDS
TCTCTTTACGAAGATCAAGT+TGG 0.545894 3.2:-87828140 MS.gene40310:CDS
TTCAAATATATACTGGTACT+CGG 0.546769 3.2:-87823481 MS.gene40310:CDS
AAGCGAGATTGGTTAAAGAG+AGG 0.551732 3.2:+87828165 None:intergenic
TACAAATGAAGGTGAGTTGG+TGG 0.553549 3.2:-87824791 MS.gene40310:CDS
CAATGCTCTGTCAATTTCAG+AGG 0.556372 3.2:-87824734 MS.gene40310:CDS
GAGGATTGTGAACATCCAGT+TGG 0.560024 3.2:-87823801 MS.gene40310:CDS
GTATACTTCCATCAGAGGAA+AGG 0.560313 3.2:+87826405 None:intergenic
GCACCAATGATGTTGTTGAT+GGG 0.562200 3.2:-87827278 MS.gene40310:CDS
ATGACCCACCACGGTTGCCA+GGG 0.562846 3.2:-87822569 MS.gene40310:CDS
TCACAAGCTGAGAAAGAAGA+AGG 0.563073 3.2:-87821824 MS.gene40310:CDS
CTATTCAAAAGCTTAGGTTG+AGG 0.563691 3.2:-87823971 MS.gene40310:CDS
AACTTTAATTAAGAGAACTT+GGG 0.565474 3.2:+87824813 None:intergenic
AGGACCCTGGCAACCGTGGT+GGG 0.566124 3.2:+87822565 None:intergenic
GTTGAATCAGTATAAGGCAG+TGG 0.566656 3.2:+87823597 None:intergenic
TCATACTTTGCGTAAATCAG+AGG 0.568075 3.2:-87823820 MS.gene40310:CDS
ACAGTTGGCGCAGTCACACT+TGG 0.569345 3.2:+87823696 None:intergenic
GAACTTACCTGACGGGCAGA+TGG 0.569560 3.2:-87826372 MS.gene40310:CDS
CTTCTTGTAATTGGGCGATG+TGG 0.570637 3.2:-87823720 MS.gene40310:CDS
GAAGTGACTTCAACTGCTAA+GGG 0.572594 3.2:-87822064 MS.gene40310:CDS
ATTGTCTTCATATTGAACAA+AGG 0.574065 3.2:+87824696 None:intergenic
TCAAGAAAAGAAATAACAGT+TGG 0.575622 3.2:+87823681 None:intergenic
CGGAACAGCAACACCAGAGG+AGG 0.576554 3.2:-87822645 MS.gene40310:CDS
GGTTCCACATCATCATGCAC+TGG 0.578057 3.2:-87823757 MS.gene40310:CDS
AGCTACAACATTCTCTTCAG+TGG 0.579214 3.2:+87827669 None:intergenic
GCAAGTTTCAGACCTCATCA+AGG 0.579316 3.2:-87824902 MS.gene40310:CDS
TTACAGCATGAACAGGACCC+TGG 0.580099 3.2:+87822552 None:intergenic
ATGGAAGTATACTGAGAGCA+TGG 0.580158 3.2:-87826394 MS.gene40310:CDS
ATATTTGGAAGTTTGTACTT+GGG 0.580879 3.2:-87822183 MS.gene40310:CDS
ATGGTCAATTGTATTCCCAT+CGG 0.582081 3.2:-87823364 MS.gene40310:CDS
GTAGGTATTTAGTGAAAACT+GGG 0.582984 3.2:-87822455 MS.gene40310:intron
GGAAGGTGTATCTGTTGCAA+AGG 0.583411 3.2:-87824407 MS.gene40310:CDS
GATTCCCCAGAAGTTGCAAA+GGG 0.590687 3.2:+87825024 None:intergenic
TCTTGATGCATACACAGGGA+AGG 0.590779 3.2:-87823664 MS.gene40310:CDS
TACAAATCTCTCCATGGCGA+AGG 0.591785 3.2:+87827638 None:intergenic
AATTGACAGAGCATTGCTAA+AGG 0.592188 3.2:+87824741 None:intergenic
TGATTCCCCAGAAGTTGCAA+AGG 0.592939 3.2:+87825023 None:intergenic
GAGCATGGAACTTACCTGAC+GGG 0.593894 3.2:-87826379 MS.gene40310:CDS
CGAGGTCTATGACCAGTCTC+GGG 0.594730 3.2:-87822380 MS.gene40310:CDS
TGATTCCTTTGATCCCTGAA+GGG 0.594748 3.2:+87826337 None:intergenic
CAGGACCCTGGCAACCGTGG+TGG 0.599458 3.2:+87822564 None:intergenic
ACTTCCCTTTGCAACTTCTG+GGG 0.600142 3.2:-87825028 MS.gene40310:CDS
GAACAGGACCCTGGCAACCG+TGG 0.601108 3.2:+87822561 None:intergenic
GGACATTTGCTGAAACTCAA+AGG 0.601815 3.2:-87824029 MS.gene40310:CDS
ATGCAGCACCAAAAGCCAGT+GGG 0.601937 3.2:-87822673 MS.gene40310:CDS
GTGAGGGATTGAGAGTCCGA+CGG 0.602706 3.2:+87821843 None:intergenic
CAATTGCATTCATGAAGTAG+TGG 0.603523 3.2:-87823415 MS.gene40310:CDS
TGTGTTCCATACTCAGAAGA+CGG 0.604040 3.2:-87827713 MS.gene40310:CDS
TTACTTATGTATTATCGGAG+AGG 0.607601 3.2:-87820565 MS.gene40310:CDS
GCTTCACTCGACCTTCGCCA+TGG 0.608681 3.2:-87827649 MS.gene40310:CDS
CAGAGCATTGATGGTAATGG+AGG 0.610246 3.2:-87824533 MS.gene40310:CDS
TTGGAATGGTGCTTTGCTGA+AGG 0.619847 3.2:-87824641 MS.gene40310:CDS
GGTCGGTAATAAGAAGAGAT+GGG 0.622823 3.2:+87822142 None:intergenic
TAGAACAGAACCTCTTCGAA+TGG 0.623515 3.2:-87824384 MS.gene40310:CDS
TCATCCAGTGCATGATGATG+TGG 0.623705 3.2:+87823753 None:intergenic
GAATAACTCGAGCAACGGTG+TGG 0.623885 3.2:+87823619 None:intergenic
AATAACTCGAGCAACGGTGT+GGG 0.627835 3.2:+87823620 None:intergenic
GTCCAGATGAATCCTTGATG+AGG 0.628352 3.2:+87824890 None:intergenic
AAGGATGTTAATGATTGGAA+TGG 0.628565 3.2:-87824655 MS.gene40310:CDS
AAATATACCTGAGAAATGAT+CGG 0.629270 3.2:+87821776 None:intergenic
AGTTACAAGAAAATCAAATG+AGG 0.629308 3.2:-87823319 MS.gene40310:intron
TAGCCATACAAATCTCTCCA+TGG 0.631175 3.2:+87827632 None:intergenic
CTACTCCATAGGCAAAGACG+AGG 0.640064 3.2:+87820699 None:intergenic
GAACTACCTGTGGAAAAGGA+AGG 0.640801 3.2:-87824424 MS.gene40310:CDS
CAGTGGAATAACTCGAGCAA+CGG 0.643016 3.2:+87823614 None:intergenic
AATGCAGCACCAAAAGCCAG+TGG 0.643369 3.2:-87822674 MS.gene40310:CDS
AGAGCATGGAACTTACCTGA+CGG 0.645707 3.2:-87826380 MS.gene40310:CDS
AGAAATTGTCTGGAGTAGAG+AGG 0.650623 3.2:-87824482 MS.gene40310:CDS
TCTCAGTATACTTCCATCAG+AGG 0.653634 3.2:+87826400 None:intergenic
GCACCAACAGTATATTGGGA+AGG 0.654684 3.2:-87827746 MS.gene40310:CDS
TTGGGCTTAACATTTATCAG+TGG 0.657781 3.2:-87823782 MS.gene40310:CDS
GTAACAATAGACATCAACAA+TGG 0.658123 3.2:-87824946 MS.gene40310:CDS
ATACCTTAAGCCATTCGAAG+AGG 0.658715 3.2:+87824374 None:intergenic
TCAGAACAGATAGATCTCAT+GGG 0.661453 3.2:-87824558 MS.gene40310:CDS
AACAACGTCAGAACTACCTG+TGG 0.662479 3.2:-87824434 MS.gene40310:CDS
TCACTTCTATTAGTTCAACA+AGG 0.662525 3.2:-87824505 MS.gene40310:CDS
TCTAATGATCGCAAGCGCTG+AGG 0.665196 3.2:-87824003 MS.gene40310:CDS
TGCAGCACCAAAAGCCAGTG+GGG 0.666440 3.2:-87822672 MS.gene40310:CDS
AATCGGAACAGCAACACCAG+AGG 0.670400 3.2:-87822648 MS.gene40310:CDS
ATTGTCTGGAGTAGAGAGGA+CGG 0.670770 3.2:-87824478 MS.gene40310:CDS
TTGGTAATAGACCATCAAAG+AGG 0.671484 3.2:-87824613 MS.gene40310:CDS
CGTTGTTACATCAATAACTG+AGG 0.681812 3.2:+87824450 None:intergenic
TGAAGTGAATGCAAAGAGCG+GGG 0.683707 3.2:-87825076 MS.gene40310:CDS
TTGCATCGCATGACCCACCA+CGG 0.684916 3.2:-87822578 MS.gene40310:CDS
GTTGCTTATAGTACTTACAA+GGG 0.685667 3.2:-87823901 MS.gene40310:CDS
TAGACGGTGAAGTTCTCTGG+AGG 0.686865 3.2:-87825292 MS.gene40310:CDS
ACATGAGATGTCAGTCATCG+AGG 0.688383 3.2:-87822398 MS.gene40310:CDS
GGTCTATGACCAGTCTCGGG+CGG 0.695398 3.2:-87822377 MS.gene40310:intron
TATGAAGTGAATGCAAAGAG+CGG 0.702657 3.2:-87825078 MS.gene40310:CDS
TACTCCATAGGCAAAGACGA+GGG 0.709340 3.2:+87820700 None:intergenic
TATACTTGTACATGACATCG+TGG 0.726568 3.2:+87822723 None:intergenic
GAGGGATTGAGAGTCCGACG+GGG 0.735305 3.2:+87821845 None:intergenic
TGTTCCATACTCAGAAGACG+GGG 0.735564 3.2:-87827711 MS.gene40310:CDS
CTAAAAGTAGAAGGTCTGCG+AGG 0.753852 3.2:-87820758 MS.gene40310:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content


Chromosome Type Strat End Strand Name
chr3.2 gene 87820538 87828228 87820538 ID=MS.gene40310
chr3.2 mRNA 87820538 87828228 87820538 ID=MS.gene40310.t1;Parent=MS.gene40310
chr3.2 exon 87828128 87828228 87828128 ID=MS.gene40310.t1.exon1;Parent=MS.gene40310.t1
chr3.2 CDS 87828128 87828228 87828128 ID=cds.MS.gene40310.t1;Parent=MS.gene40310.t1
chr3.2 exon 87827641 87827768 87827641 ID=MS.gene40310.t1.exon2;Parent=MS.gene40310.t1
chr3.2 CDS 87827641 87827768 87827641 ID=cds.MS.gene40310.t1;Parent=MS.gene40310.t1
chr3.2 exon 87827256 87827318 87827256 ID=MS.gene40310.t1.exon3;Parent=MS.gene40310.t1
chr3.2 CDS 87827256 87827318 87827256 ID=cds.MS.gene40310.t1;Parent=MS.gene40310.t1
chr3.2 exon 87826316 87826437 87826316 ID=MS.gene40310.t1.exon4;Parent=MS.gene40310.t1
chr3.2 CDS 87826316 87826437 87826316 ID=cds.MS.gene40310.t1;Parent=MS.gene40310.t1
chr3.2 exon 87825272 87825387 87825272 ID=MS.gene40310.t1.exon5;Parent=MS.gene40310.t1
chr3.2 CDS 87825272 87825387 87825272 ID=cds.MS.gene40310.t1;Parent=MS.gene40310.t1
chr3.2 exon 87824378 87825179 87824378 ID=MS.gene40310.t1.exon6;Parent=MS.gene40310.t1
chr3.2 CDS 87824378 87825179 87824378 ID=cds.MS.gene40310.t1;Parent=MS.gene40310.t1
chr3.2 exon 87823320 87824051 87823320 ID=MS.gene40310.t1.exon7;Parent=MS.gene40310.t1
chr3.2 CDS 87823320 87824051 87823320 ID=cds.MS.gene40310.t1;Parent=MS.gene40310.t1
chr3.2 exon 87822556 87822777 87822556 ID=MS.gene40310.t1.exon8;Parent=MS.gene40310.t1
chr3.2 CDS 87822556 87822777 87822556 ID=cds.MS.gene40310.t1;Parent=MS.gene40310.t1
chr3.2 exon 87822378 87822473 87822378 ID=MS.gene40310.t1.exon9;Parent=MS.gene40310.t1
chr3.2 CDS 87822378 87822473 87822378 ID=cds.MS.gene40310.t1;Parent=MS.gene40310.t1
chr3.2 exon 87822021 87822215 87822021 ID=MS.gene40310.t1.exon10;Parent=MS.gene40310.t1
chr3.2 CDS 87822021 87822215 87822021 ID=cds.MS.gene40310.t1;Parent=MS.gene40310.t1
chr3.2 exon 87821784 87821897 87821784 ID=MS.gene40310.t1.exon11;Parent=MS.gene40310.t1
chr3.2 CDS 87821784 87821897 87821784 ID=cds.MS.gene40310.t1;Parent=MS.gene40310.t1
chr3.2 exon 87820538 87820798 87820538 ID=MS.gene40310.t1.exon12;Parent=MS.gene40310.t1
chr3.2 CDS 87820538 87820798 87820538 ID=cds.MS.gene40310.t1;Parent=MS.gene40310.t1
Gene Sequence

>MS.gene40310

ATGGCCATGGCGATTAGGGTTTTTCTCTCTTTTCTCCTTTTCCTCTCTTTAACCAATCTCGCTTCTTCTCTTTACGAAGATCAAGTTGGTCTCATGGATTGGTAAATTCTATCACCCTTATTCACACCACAATTCCATTTGTTTTAATTATTCCCTTGTTGTAATCATCGCCTTAAAACCTCGATCTATTTTCATTGATTTTATTTCTAATTTCAAACTCATCGATGTTGTTACAAGTACTTGCTACTTAATCTAAACCTTAACTGATGTTGATCGTTTTTTTTTTAATCGTTGAGGATAATCAATTTTACACGGAAAAATAAACTTCTAGTGCATAAATTAATCTCATCTATTCTATATGTGTGACTTTAAAAGTGATTATAATCAAATTCAATCTGTTTTTATGATTTGGAAGTTATAATTGATGGATTGTGATTATGTTTTTGGCTGCCAATTGCAGGCACCAACAGTATATTGGGAAGGTGAAGCATGCTGTGTTCCATACTCAGAAGACGGGGCGAAAGCGTGTTTTAGTGTCCACTGAAGAGAATGTTGTAGCTTCACTCGACCTTCGCCATGGAGAGATTTGTATGGCTAATTTGTGTTACTGTTTTTTCAGTATATTAATTAAGCTGTTATCCGAACTAAATATAAGAGAAATTTGGGTCAACAAAAGTTGATGTATCTAGTCCAAACCCAAATTTCTGTTATATTTAGGAGAGTGTTGTTTTTGATACTGCCATAGGTTATGGCTTATGATGGTTAATATAATATTGAGCCTAATAGTCTTGAATCGTTTTCGGAGTAAATCAAGCCTTTTCCAAGCAATAACCATTGGTTGTTTGCTTAGTTGTTTGTTTTCTTTTGTTTGGAATTAGAAAATGATGAATTGTGTTTTTGTTGCTTGCAGTTTGGAGGCATGTTCTTGGCACCAATGATGTTGTTGATGGGATTGACATTGCCCTTGGAAAATGTATGATGTCTAACCTTTTTCTGTTTGGTATCTTTTCACGCAGAATGATACTACATGCTCTCTAGTTTTAGTTGTACTGCAGAAAAGTTATATTGTAGTTTTATAATTTATCATAAAAAATATATCGACATTGGGATGTGACATTAATTTTCAGGGTTCTTCTTGTTACCACGATCCCTTTCTATTCCTTTTTTGGTACTCAGTTTAGTTATTTGAAATTTGTATTTCTTTTGAAAATATATTTGTTTGGATTTCTTCTACTAACATATTTGACGAAGAACAAGTTATTGACCAAAATATAACAAATATATGTGATATATTTCCTCATAAATACAGTCTTCCTGTTGTTGCATTCTCTGTTGCAATTCTCATACGAGGTTGCAAATGAAGATGTTGGGGTTGTAGATCTAAAACTTTGGTGAATCAATCTGATGCTCGTCTCAGTCTCACTCTAATTGCATAAATATATCAAAGAGTAAACATCTTTCTCATAAAAGAGTAAAGTTAGCCTGAGATTCACTCCACCTCGTTTCCACTTTCAAGTGTCTATTGTCCAAAAATCTCATTTGGCTATCTGTATCTCGTAAAGCAATGATATTCCTCGCGATTGTTGTCTCATTGTTTCAAATATAATATAATAAGTTAAAGGATGTCATGCACTAGTAAAAAGACTTAACAGTGATTTTTCATTAATTTGTCATTTCTTCTGTGCTCTCTTTAATACTTTGTTGGAGTGAATTATTTTATGCATCAAGATTTGTTTCAATTTTAGTGTATATTAGTGATTGAGTTCACAAATGTGTTTTTAAATGTTGCAGATGTCATCACCCTTTCCTCTGATGGAAGTATACTGAGAGCATGGAACTTACCTGACGGGCAGATGGTTTGGGAGTCTTCCCTTCAGGGATCAAAGGAATCAAAGTCAATATTAAACATTCCAGTAAGTTTTTTTTTTTTTCTTTCTAAGTACCATATCTTTTGTTATATACATATGAATGTGATGTGGTTTCTTATTGAATAAAGTAAGAAGATTACGAAAAGGTTAAGAAACAATAACTGATATAAAAGGTATAACATCCTCCAAAGCTGTGAAAAGGACACATACATAAAGTGAGCATCCTCTGGAGATGCAAACAGAACTCAATAATAGATTGAACTAGTTAAAACAATAGACCTTCTAATTCTCTCGAACATAATTAGAGAAATCCTCCTTAATATATGCATTAAGATGAATGCCAAAGCAAATAAGGTGCCATGACTCGACCCATATTATGGGTGACTTTGCTATGTGGGCACTGTTTGGGAGTTTGTAGGGGAGGGAGGGGGGAAGAGAGAAATCTCCCACCTTATTTAAGAGAGTCATTTTGTAGAGAATGAGAAGAGAAAGCTTATGATTGATATATTTTTAAGAGTATTATAAAAGGATGGAAATGATCTGAAGAATTTTTTTAAACCCTCAGAAAGCTTTCTGCAATATATCGAGCTCACCCATATTATGGGGATTTGGTATTATGAAGAAAAATAAACCCCCATTCAAGTTGGGAGACATTCCTTCTCTTTCTCTTTTCCCTTTCCTTTCCTTTCCCCCAAAGCCCTTCCCTCCTGTTCTAAACCCCCAAGCGGAGCCTTAAGGTCTTTATTTTATTGAAATTTTTCATTGGCAGTGTGTTCGCTCAACCAACCCATTTTCTAAAATGGGCTAACCTACCCGCCCTTTCATTGGCTGGTTGATGGTGGGGCAGAATAGTTTAGTCTGCATTGCCATCTTTATGAACCTGCACTGACCCCCTTTACTGCAGTTGGCCTTGGTTTATTTTGTTGTATTTTTCCGTACACCTGTAATTAATTGCAGTTTTTACTTTTTGCAGAAAAATTTGAAAGCTGACAAGGATGATTTGATACTTGTTTTTGGTAAAGGGTGTCTTCATGCCATTTCTGGCATAGACGGTGAAGTTCTCTGGAGGAAAGATTTTGCTAGTGAGAGGTATGACAAATTTGGGGTTGGATAAGCCTGATATTTTTTTCTTAAGCGAAAGAGTACCATTGATTTTTTTTATATCTTAATCATATTTGCAGCATTGAAGTTAGCCATATTATACAGTCTCCTGATGTGATTTATGTAGCTGGTTTTGTTGGTTCTTTAAAGTTTTATGTTTATGAAGTGAATGCAAAGAGCGGGGAGTTGTTAAAGAATAATCATGTAGCACTTCCCTTTGCAACTTCTGGGGAATCATTATCAGTCTCAGGTGATAAGTTTGTGGTGTTGGATGATGTAAGGTCAAAGATAGTAACAATAGACATCAACAATGGAGATATTAACTACAACCAGAAGCAAGTTTCAGACCTCATCAAGGATTCATCTGGACAGGCAGTAATATTACCATCAAAGCTTCCAGGACTGTTTGCATTAAAAATCAATTCCCAAGTTCTCTTAATTAAAGTTACAAATGAAGGTGAGTTGGTGGCGCTGGACCAAATCGACAATACAGCAGCCTTTAGCAATGCTCTGTCAATTTCAGAGGATCAACATGTCTTTGCCTTTGTTCAATATGAAGACAATAAAATTCAGCTCTCTGTAAAGGATGTTAATGATTGGAATGGTGCTTTGCTGAAGGAAAACTTGGTAATAGACCATCAAAGAGGAAATATTGAGAAGATTTTCATAAACAATTATGTCAGAACAGATAGATCTCATGGGTTCAGAGCATTGATGGTAATGGAGGATCATTCACTTCTATTAGTTCAACAAGGAGAAATTGTCTGGAGTAGAGAGGACGGTCTTGCCTCAGTTATTGATGTAACAACGTCAGAACTACCTGTGGAAAAGGAAGGTGTATCTGTTGCAAAGGTAGAACAGAACCTCTTCGAATGGCTTAAGGTATGCCCTTGATATGTTTAACAAGGACATTATACTGTCTATTTTTTTACAGATATTTATGTTTTTGCGTGTGTATGTGTCAATTGAGAGTACATCTCATTTGAAGAATTAATCTTGACCACACAACATAATTATATGATCAAGATTATCTTGAAGTAAACAATTTTGACTGTTAGTGTATGTTTATGTGGTCATCAAGATTAGACCCTCTCACACTTCCGCTTGATACTTCTCTATATGTATATATTCTGTTGTTTTAATGTTAGAATTATGATTTTTATGTGGATATCATTAACTGCTGCTCTAATGGTGGAACTTTCATGAAGGGACATTTGCTGAAACTCAAAGGAACTCTAATGATCGCAAGCGCTGAGGAGAAAATAGCTATTCAAAAGCTTAGGTTGAGGAGTTCTGAGAAAAGCAAAATGACTCGTGATCATAATGGTTTTCGTAAGTTGCTTATAGTACTTACAAGGGCAGGAAAGGTTTTTGCTTTGCATACCGGGGATGGACGCATAGTTTGGTCTACGACACTTCATACTTTGCGTAAATCAGAGGATTGTGAACATCCAGTTGGGCTTAACATTTATCAGTGGCAGGTTCCACATCATCATGCACTGGATGAGAATCCATCTCTTCTTGTAATTGGGCGATGTGGACCAAGTGTGACTGCGCCAACTGTTATTTCTTTTCTTGATGCATACACAGGGAAGGAACTCAATTCTCTGAGTCTTGCCCACACCGTTGCTCGAGTTATTCCACTGCCTTATACTGATTCAACTGAACAGCGTCTGCATCTTATTATAGATGTTAACAAACATGCATATTTATACCCAAGAACTCCTGAAGCTATTGAGATTCTGAAACGTGAGTTTTCAAATATATACTGGTACTCGGTTGAGACTGATAATGGTGTTATTAGAGGTCATGCATTAAAGAGCAATTGCATTCATGAAGTAGTGGATGAATACTGCTTTGTATTCAGGGACTTATGGTCAATTGTATTCCCATCGGAATCAGAAAAGATTATTGCAACAGTTACAAGAAAATCAAATGAGGTATGGTGCCTTTGGTTTTGAGGTTTTTAATTAAAAGAACATTTTTTGTTATATTCTACTTCATCTAATACAGATCACCTTATGTTTTAAAAATATTAGTCTCCCATCTGAGGTATATAAACATGCCTTACTCGAAATACTTTTCTTTTAAATTTAATGAGTATGTGCTCAACTCGACAGCTTGAGTTGGGTTTTATCATAAATAAATCAATGTCACTATAAAGAATGAAATTAAGTTTTTAGTCTTGAGGTGATTTTTTTTCCGAGTAAGAGGTGATTTGATTGGTAGTGTAATATTTACTAATCTTAATATCCAAGTTTTGTTATGCTCCCAAATCAGCGTGCCTTTAGTTTTTCCGAAGTCGTTTGAATTGTATATGTAGTTATATTTCAATATAACAAATGATCATAAATAATACGACAATTAAAGATTTTCAACGCACTTAAACTTTAAATATTATAATGACTCTCTTTCATCGAGTCTTTGGTCATTTCTTTGCTTCCTTCCTTTCTAAATTATTATTCTCTTTTGTTTCATCTAGGTTGTTCATACTCAAGCAAAAGTTATGACTGACCACGATGTCATGTACAAGTATATATCGAAGAATATACTTTTTGTCGCAAATGCAGCACCAAAAGCCAGTGGGGAAATCGGAACAGCAACACCAGAGGAGGCAACTTTGGTTATCTATATCATTGATACTGTGACTGGTCGCATATTGCATCGCATGACCCACCACGGTTGCCAGGGTCCTGTTCATGCTGTAAGCTTTTGAGCTACCTTCTCATTCTTAAGATGTATTATATGAATCTGGCTCTCACATCGCATGAATATTATTTCTGTAGGTATTTAGTGAAAACTGGGTTGTCTACCACTATTTTAATCTCAGGGCTCACAGACATGAGATGTCAGTCATCGAGGTCTATGACCAGTCTCGGGCGGTATGTCAGCCAACTTGTGTATTGTTATTTGATTATTCTTCTCTCTGTCTAATTTTTTGCTGCTAATCTTTTTTTACATGGTTATTGTGTTCATCCAGTCATTATTTCTTGTGTTGTCTTTGTTCATTCTAATGTGATATATGGCTGGAATATATTTCACAGGATAACAAAGATATTTGGAAGTTTGTACTTGGGAAGCATAATCTTACATCACCCATCTCTTCTTATTACCGACCAGAAATCTCAGCAAAATCACAATCATACTTCTTTACTCACTCTGTGAAAGCCATTGAAGTGACTTCAACTGCTAAGGGTATAACCTCTAAGCAGCTTCTTATCGGAACAATTGGTGATCAGGTAGGAATTTATTATGACTGTTTTCCCACATGAATACTTGAGATCAAAAGCAACACTCTGTTGTTTTCTCATTTAATTGGATTCTTCAATGACATTCTTACTGTTTTTTTTTGTTTGCTGCAGGTTTTGGCACTTGATAAACGATTTTTGGATCCCCGTCGGACTCTCAATCCCTCACAAGCTGAGAAAGAAGAAGGAATTATTCCTCTTACTGATTCACTACCGATCATTTCTCAGGTATATTTTCATCTGAAGTTTCCTTAATTTAATACAAGCAAAAAAAAATCCTTCATTATCTCAAAAGAAATTTGTTGAGTCTTTAAAGGATTAAAAACATCAGCAAGTTTACAAATTTCTTTTATCACAATAAATATGAAATTGCTTCTGGTCAGGGAGACAGTGCGTTGAGAAGAGGGGGGATGGGTTGGGGGGACATATTTTGGATGACAAAGAATTATCTACTGATGAAGGGATGTGACCTTTATTCTTTAGATATTTAGCGGCAAAGTAGATAACATGCCAAGTTATAGCCAGCAATATATATCTAAGGGTGGGCAAAAAAACCAAAAAACCGAACTGAACTGCTGAACTTTACTGAACCAAAAAAAACTGAACTATTACTAACAGTTCTGAAACTGAACTGAAACAGTTCAGTTCGGTTCAAAACAGTTCGGTTCAGTTCGGTGACTAAAATTCGATTTCGTTTGTTTTTATTCATAATTTTTTTCAAAGGTATCACTAATAACAGTTCGGTTCAGTTCAGTTTGAAACAGTTCAGTTATTTTAAATTTAGCAAACAGTTCAGTTTGGTAGTTTTCATCCCAAACTGAACCATGCCCACATGCATGTTGTGTGAGATTAATTTCTGATTTAACTATATATATAACCCCCCATTCCTTTTTCTCGGGGGAATTTCTCTGTACCAGAGGGATTAGCCATATGTTAGACAGTAATGAATTACAACTTTAATAACTTGTTCCTATCCATTATGAGAACTAGTAATTGGAAAATGATAAGGAGGATGGAGAAATGACTTTGAGCCCTGTTTTTACTTGCTATGGCCTGCACTTGCATATCGTCCTTCCTCAGTTTAGAGTAGATCTGCTTGAGCAAATGCATACTAGTTAGATAAACATTTGAAACCAACTTGGTCTCATGCTTATCTTTAAGCTTACTATACTTCTTAACATGAAATTCACACATTATGTTTGAATCTATGCAGTCCTATATCACACATTCGCTAAAAGTAGAAGGTCTGCGAGGTATAGTAACAGTGCCTGCCAAATTGGAGTCTACATCCCTCGTCTTTGCCTATGGAGTAGATCTATTTTTTACTCAGATTGCTCCTTCTAGGACTTACGATTCACTGACTGAAGATTTCAGCTATGCTCTACTTCTGCTAACAATTGTTGCACTTGTAGCAGCATTATTTGTTACTTATGTATTATCGGAGAGGAAAGATCTAGAAGAGAAATGGAGATGA
Protein sequence

>MS.gene40310.t1

MAMAIRVFLSFLLFLSLTNLASSLYEDQVGLMDWHQQYIGKVKHAVFHTQKTGRKRVLVSTEENVVASLDLRHGEIFWRHVLGTNDVVDGIDIALGKYVITLSSDGSILRAWNLPDGQMVWESSLQGSKESKSILNIPKNLKADKDDLILVFGKGCLHAISGIDGEVLWRKDFASESIEVSHIIQSPDVIYVAGFVGSLKFYVYEVNAKSGELLKNNHVALPFATSGESLSVSGDKFVVLDDVRSKIVTIDINNGDINYNQKQVSDLIKDSSGQAVILPSKLPGLFALKINSQVLLIKVTNEGELVALDQIDNTAAFSNALSISEDQHVFAFVQYEDNKIQLSVKDVNDWNGALLKENLVIDHQRGNIEKIFINNYVRTDRSHGFRALMVMEDHSLLLVQQGEIVWSREDGLASVIDVTTSELPVEKEGVSVAKVEQNLFEWLKGHLLKLKGTLMIASAEEKIAIQKLRLRSSEKSKMTRDHNGFRKLLIVLTRAGKVFALHTGDGRIVWSTTLHTLRKSEDCEHPVGLNIYQWQVPHHHALDENPSLLVIGRCGPSVTAPTVISFLDAYTGKELNSLSLAHTVARVIPLPYTDSTEQRLHLIIDVNKHAYLYPRTPEAIEILKREFSNIYWYSVETDNGVIRGHALKSNCIHEVVDEYCFVFRDLWSIVFPSESEKIIATVTRKSNEVVHTQAKVMTDHDVMYKYISKNILFVANAAPKASGEIGTATPEEATLVIYIIDTVTGRILHRMTHHGCQGPVHAVFSENWVVYHYFNLRAHRHEMSVIEVYDQSRADNKDIWKFVLGKHNLTSPISSYYRPEISAKSQSYFFTHSVKAIEVTSTAKGITSKQLLIGTIGDQVLALDKRFLDPRRTLNPSQAEKEEGIIPLTDSLPIISQSYITHSLKVEGLRGIVTVPAKLESTSLVFAYGVDLFFTQIAPSRTYDSLTEDFSYALLLLTIVALVAALFVTYVLSERKDLEEKWR