Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058550.t1 | XP_003630965.1 | 100 | 616 | 0 | 0 | 1 | 616 | 1 | 616 | 0.00E+00 | 1183.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058550.t1 | Q8LF21 | 86.7 | 617 | 78 | 2 | 1 | 616 | 1 | 614 | 2.3e-310 | 1065.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058550.t1 | G7LBF4 | 100.0 | 616 | 0 | 0 | 1 | 616 | 1 | 616 | 0.0e+00 | 1183.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050818 | MS.gene058550 | 0.811225 | 7.79E-51 | -1.69E-46 |
| MS.gene050894 | MS.gene058550 | 0.817869 | 2.64E-52 | -1.69E-46 |
| MS.gene054585 | MS.gene058550 | 0.81027 | 1.25E-50 | -1.69E-46 |
| MS.gene058550 | MS.gene060077 | 0.804603 | 2.00E-49 | -1.69E-46 |
| MS.gene058550 | MS.gene06366 | 0.811039 | 8.55E-51 | -1.69E-46 |
| MS.gene058550 | MS.gene28286 | 0.815288 | 9.98E-52 | -1.69E-46 |
| MS.gene058550 | MS.gene29353 | 0.814623 | 1.40E-51 | -1.69E-46 |
| MS.gene058550 | MS.gene30247 | 0.857112 | 2.04E-62 | -1.69E-46 |
| MS.gene058550 | MS.gene40310 | 0.801195 | 1.01E-48 | -1.69E-46 |
| MS.gene058550 | MS.gene41692 | 0.802523 | 5.39E-49 | -1.69E-46 |
| MS.gene058550 | MS.gene53779 | 0.805066 | 1.60E-49 | -1.69E-46 |
| MS.gene058550 | MS.gene53975 | 0.818216 | 2.20E-52 | -1.69E-46 |
| MS.gene058550 | MS.gene55148 | 0.8065 | 7.98E-50 | -1.69E-46 |
| MS.gene058550 | MS.gene56457 | 0.846104 | 2.68E-59 | -1.69E-46 |
| MS.gene058550 | MS.gene62680 | 0.85731 | 1.78E-62 | -1.69E-46 |
| MS.gene058550 | MS.gene64717 | 0.829607 | 4.68E-55 | -1.69E-46 |
| MS.gene058550 | MS.gene67231 | 0.821922 | 3.12E-53 | -1.69E-46 |
| MS.gene058550 | MS.gene82399 | 0.815978 | 7.01E-52 | -1.69E-46 |
| MS.gene058550 | MS.gene82400 | 0.828179 | 1.04E-54 | -1.69E-46 |
| MS.gene058550 | MS.gene96049 | 0.813775 | 2.16E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene058550 | MS.gene035805 | PPI |
| MS.gene058550 | MS.gene73119 | PPI |
| MS.gene058550 | MS.gene064542 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058550.t1 | MTR_8g105580 | 100.000 | 616 | 0 | 0 | 1 | 616 | 1 | 616 | 0.0 | 1273 |
| MS.gene058550.t1 | MTR_8g105580 | 100.000 | 575 | 0 | 0 | 1 | 575 | 1 | 575 | 0.0 | 1189 |
| MS.gene058550.t1 | MTR_1g032550 | 88.006 | 617 | 71 | 2 | 1 | 616 | 1 | 615 | 0.0 | 1123 |
| MS.gene058550.t1 | MTR_8g009510 | 76.220 | 635 | 131 | 5 | 1 | 615 | 1 | 635 | 0.0 | 983 |
| MS.gene058550.t1 | MTR_7g451010 | 78.135 | 622 | 122 | 5 | 1 | 615 | 1 | 615 | 0.0 | 979 |
| MS.gene058550.t1 | MTR_4g098920 | 71.707 | 615 | 161 | 4 | 4 | 615 | 1 | 605 | 0.0 | 932 |
| MS.gene058550.t1 | MTR_8g099550 | 66.505 | 618 | 191 | 4 | 4 | 615 | 1 | 608 | 0.0 | 890 |
| MS.gene058550.t1 | MTR_5g009150 | 66.074 | 619 | 193 | 4 | 4 | 615 | 1 | 609 | 0.0 | 884 |
| MS.gene058550.t1 | MTR_8g027675 | 66.450 | 614 | 197 | 3 | 4 | 615 | 1 | 607 | 0.0 | 871 |
| MS.gene058550.t1 | MTR_8g009510 | 76.281 | 527 | 111 | 3 | 103 | 615 | 2 | 528 | 0.0 | 822 |
| MS.gene058550.t1 | MTR_2g009690 | 38.337 | 493 | 284 | 6 | 5 | 487 | 22 | 504 | 2.92e-97 | 316 |
| MS.gene058550.t1 | MTR_4g093750 | 38.134 | 493 | 285 | 6 | 5 | 487 | 13 | 495 | 2.93e-94 | 305 |
| MS.gene058550.t1 | MTR_4g093750 | 38.057 | 494 | 286 | 6 | 5 | 487 | 13 | 497 | 3.05e-93 | 303 |
| MS.gene058550.t1 | MTR_4g030140 | 31.262 | 515 | 320 | 9 | 1 | 495 | 1 | 501 | 1.38e-66 | 234 |
| MS.gene058550.t1 | MTR_4g134680 | 29.746 | 511 | 330 | 9 | 1 | 495 | 1 | 498 | 5.00e-65 | 228 |
| MS.gene058550.t1 | MTR_4g134680 | 29.746 | 511 | 330 | 9 | 1 | 495 | 1 | 498 | 2.72e-64 | 227 |
| MS.gene058550.t1 | MTR_4g134680 | 29.746 | 511 | 330 | 9 | 1 | 495 | 1 | 498 | 8.86e-64 | 226 |
| MS.gene058550.t1 | MTR_4g134680 | 36.869 | 198 | 125 | 0 | 295 | 492 | 1 | 198 | 1.52e-37 | 148 |
| MS.gene058550.t1 | MTR_8g087770 | 35.816 | 282 | 162 | 6 | 35 | 310 | 68 | 336 | 1.02e-33 | 137 |
| MS.gene058550.t1 | MTR_8g087750 | 35.714 | 294 | 166 | 8 | 35 | 320 | 71 | 349 | 1.57e-33 | 136 |
| MS.gene058550.t1 | MTR_8g463270 | 25.698 | 358 | 222 | 9 | 12 | 341 | 31 | 372 | 1.30e-17 | 87.4 |
| MS.gene058550.t1 | MTR_4g022340 | 26.552 | 290 | 158 | 9 | 38 | 302 | 32 | 291 | 1.52e-13 | 72.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058550.t1 | AT1G14830 | 87.702 | 618 | 70 | 3 | 1 | 616 | 1 | 614 | 0.0 | 1130 |
| MS.gene058550.t1 | AT3G60190 | 77.244 | 624 | 131 | 4 | 1 | 615 | 1 | 622 | 0.0 | 1015 |
| MS.gene058550.t1 | AT2G44590 | 74.351 | 616 | 148 | 3 | 4 | 615 | 1 | 610 | 0.0 | 966 |
| MS.gene058550.t1 | AT2G44590 | 71.591 | 616 | 148 | 4 | 4 | 615 | 1 | 593 | 0.0 | 922 |
| MS.gene058550.t1 | AT2G44590 | 71.475 | 617 | 148 | 5 | 4 | 615 | 1 | 594 | 0.0 | 919 |
| MS.gene058550.t1 | AT3G61760 | 66.829 | 615 | 194 | 5 | 4 | 615 | 1 | 608 | 0.0 | 881 |
| MS.gene058550.t1 | AT5G42080 | 66.829 | 615 | 194 | 4 | 4 | 615 | 1 | 608 | 0.0 | 879 |
| MS.gene058550.t1 | AT5G42080 | 66.829 | 615 | 194 | 4 | 4 | 615 | 1 | 608 | 0.0 | 879 |
| MS.gene058550.t1 | AT5G42080 | 66.179 | 615 | 192 | 5 | 4 | 615 | 1 | 602 | 0.0 | 864 |
| MS.gene058550.t1 | AT3G61760 | 67.652 | 575 | 176 | 5 | 4 | 575 | 1 | 568 | 0.0 | 830 |
| MS.gene058550.t1 | AT3G61760 | 72.297 | 444 | 120 | 3 | 4 | 444 | 1 | 444 | 0.0 | 695 |
| MS.gene058550.t1 | AT5G42080 | 73.660 | 429 | 110 | 2 | 4 | 429 | 1 | 429 | 0.0 | 681 |
| MS.gene058550.t1 | AT2G14120 | 38.477 | 499 | 281 | 8 | 5 | 487 | 19 | 507 | 1.97e-97 | 314 |
| MS.gene058550.t1 | AT2G14120 | 38.477 | 499 | 281 | 8 | 5 | 487 | 19 | 507 | 9.73e-97 | 313 |
| MS.gene058550.t1 | AT2G14120 | 38.477 | 499 | 281 | 8 | 5 | 487 | 19 | 507 | 9.83e-97 | 313 |
| MS.gene058550.t1 | AT4G33650 | 38.229 | 497 | 284 | 8 | 5 | 487 | 35 | 522 | 4.50e-93 | 304 |
| MS.gene058550.t1 | AT4G33650 | 38.229 | 497 | 284 | 8 | 5 | 487 | 35 | 522 | 4.74e-93 | 304 |
| MS.gene058550.t1 | AT2G14120 | 36.742 | 528 | 279 | 9 | 5 | 487 | 19 | 536 | 7.89e-93 | 303 |
| MS.gene058550.t1 | AT1G10290 | 30.934 | 514 | 320 | 10 | 1 | 495 | 1 | 498 | 6.29e-66 | 232 |
| MS.gene058550.t1 | AT1G59610 | 32.558 | 473 | 289 | 10 | 38 | 495 | 41 | 498 | 1.84e-64 | 228 |
| MS.gene058550.t1 | AT1G60500 | 24.355 | 620 | 410 | 15 | 35 | 614 | 65 | 665 | 1.98e-40 | 157 |
| MS.gene058550.t1 | AT1G60530 | 42.703 | 185 | 105 | 1 | 35 | 219 | 62 | 245 | 4.99e-39 | 145 |
| MS.gene058550.t1 | AT1G53140 | 26.741 | 359 | 209 | 11 | 12 | 334 | 47 | 387 | 1.21e-18 | 90.9 |
Find 154 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGGACATTTGGCGCATAAAA+TGG | 0.173187 | 4.4:-1882182 | MS.gene058550:CDS |
| CGACTCTGTGGCTTGGAAAT+AGG | 0.183632 | 4.4:-1878431 | None:intergenic |
| ATCAATTGTCTTGTTTATTA+AGG | 0.199022 | 4.4:+1881953 | None:intergenic |
| GGAGCATTCCGGTTTGAATT+TGG | 0.210028 | 4.4:+1880089 | None:intergenic |
| TGATACGCAGAAGGAGAAAT+TGG | 0.255509 | 4.4:-1878545 | MS.gene058550:intron |
| GTGGCGAAGGTTTATCTCTT+TGG | 0.255931 | 4.4:-1886463 | MS.gene058550:CDS |
| GGTTACAAAACTTGATCTTA+TGG | 0.260054 | 4.4:-1882493 | MS.gene058550:CDS |
| TGCGCCAAATGTCCATATTC+TGG | 0.263377 | 4.4:+1882190 | None:intergenic |
| TGCACGACACATTTCTAAAA+TGG | 0.282163 | 4.4:+1881764 | None:intergenic |
| GATGAAACAGATCGAATAAC+TGG | 0.291503 | 4.4:-1885115 | MS.gene058550:CDS |
| CAACTTCATAAGACAGAAAA+TGG | 0.300140 | 4.4:-1885645 | MS.gene058550:CDS |
| TTTGAATTTGGTGGTCCATT+TGG | 0.308876 | 4.4:+1880101 | None:intergenic |
| GACTCTGTGGCTTGGAAATA+GGG | 0.314338 | 4.4:-1878430 | None:intergenic |
| TGACAATGGACTACGGCTTT+TGG | 0.316072 | 4.4:+1879250 | None:intergenic |
| AGGCCGATTGCAGTGGATTC+CGG | 0.317259 | 4.4:-1881900 | MS.gene058550:CDS |
| TTTGAGACTAGTCCAGAATA+TGG | 0.319814 | 4.4:-1882202 | MS.gene058550:CDS |
| GGCCGATTGCAGTGGATTCC+GGG | 0.320029 | 4.4:-1881899 | MS.gene058550:CDS |
| TTGAATTTGGTGGTCCATTT+GGG | 0.329073 | 4.4:+1880102 | None:intergenic |
| TCAGTAAAATTATCATTGTT+GGG | 0.334634 | 4.4:+1880065 | None:intergenic |
| AGTTACGCGGCTGGTTGATA+TGG | 0.336032 | 4.4:-1880183 | MS.gene058550:CDS |
| ATCTAACAGCAGAATTCTTT+AGG | 0.338181 | 4.4:-1880151 | MS.gene058550:CDS |
| TTTCTAAAATGGTGTATAGT+TGG | 0.343193 | 4.4:+1881775 | None:intergenic |
| GCATCCACGGAGGCTTCTGC+TGG | 0.348132 | 4.4:+1881369 | None:intergenic |
| GTCAGTAAAATTATCATTGT+TGG | 0.352098 | 4.4:+1880064 | None:intergenic |
| TTCTTGAAGGGCGGCAATAC+AGG | 0.352799 | 4.4:-1882321 | MS.gene058550:CDS |
| AAGTCGGTGAATCTCTTTCT+TGG | 0.356235 | 4.4:+1885594 | None:intergenic |
| AGCATTTGGAGCAAGTTATC+AGG | 0.360469 | 4.4:-1881998 | MS.gene058550:CDS |
| AAACTTGATCTTATGGATAA+AGG | 0.363059 | 4.4:-1882486 | MS.gene058550:CDS |
| ATTTCAGGTGAAAGAACATT+TGG | 0.368040 | 4.4:-1882519 | MS.gene058550:intron |
| TGGCGAAGGTTTATCTCTTT+GGG | 0.372210 | 4.4:-1886462 | MS.gene058550:CDS |
| TTCTAAAATGGTGTATAGTT+GGG | 0.382131 | 4.4:+1881776 | None:intergenic |
| CCTGGTGGGGACCGGATATA+CGG | 0.382856 | 4.4:-1881567 | MS.gene058550:CDS |
| GACTTTCTTCCTCGTGGATC+TGG | 0.386806 | 4.4:-1886362 | MS.gene058550:intron |
| GAATAAATGCTGAGTTGAAT+AGG | 0.388151 | 4.4:+1885067 | None:intergenic |
| GTAGTTTAGCAATGATCTCT+TGG | 0.401450 | 4.4:+1879216 | None:intergenic |
| GCAAAAGAAGTCGATCCTTC+AGG | 0.402730 | 4.4:-1882666 | MS.gene058550:intron |
| GAAGAAACTCAGCATATTCC+TGG | 0.407055 | 4.4:+1885622 | None:intergenic |
| GTCGTCGGTGGACAGAGTTC+AGG | 0.407717 | 4.4:-1886419 | MS.gene058550:CDS |
| TGTTCAAGATATTGAACAAA+TGG | 0.409474 | 4.4:-1884098 | MS.gene058550:CDS |
| TGTAAGTGCTTATATCAATA+TGG | 0.415690 | 4.4:-1879299 | MS.gene058550:CDS |
| TTTATTAAGGCAATGATACT+AGG | 0.426555 | 4.4:+1881966 | None:intergenic |
| GACAATCACCTCCGCAAGAT+TGG | 0.428586 | 4.4:-1880044 | MS.gene058550:intron |
| TCTTGCTAGGCTTCTATCTC+AGG | 0.429303 | 4.4:-1882149 | MS.gene058550:intron |
| GGATGAAGACCCATCCCTAA+TGG | 0.431010 | 4.4:-1878512 | MS.gene058550:CDS |
| CTCAGCATTTATTCTCCAAA+TGG | 0.434296 | 4.4:-1885058 | MS.gene058550:intron |
| TTAACCCTCATAGATCTTCC+TGG | 0.442577 | 4.4:-1884272 | MS.gene058550:CDS |
| TTCAGGAAAATCCTCTGTCT+TGG | 0.443738 | 4.4:-1886402 | MS.gene058550:CDS |
| GGACATTTGGCGCATAAAAT+GGG | 0.448357 | 4.4:-1882181 | MS.gene058550:CDS |
| TCATCTGATTGCTCCAGAGC+AGG | 0.454372 | 4.4:-1881436 | MS.gene058550:CDS |
| TCTGTCTTGGAAAGTGTTGT+TGG | 0.456396 | 4.4:-1886389 | MS.gene058550:CDS |
| ATCTTGATTGGCGGGAGAGA+TGG | 0.457501 | 4.4:+1882716 | None:intergenic |
| TTATGTTCAAGTTGGAAAGA+AGG | 0.468501 | 4.4:-1879191 | MS.gene058550:CDS |
| TAGTCCAGAATATGGACATT+TGG | 0.470149 | 4.4:-1882194 | MS.gene058550:CDS |
| ACCCCGGAATCCACTGCAAT+CGG | 0.472822 | 4.4:+1881897 | None:intergenic |
| TGAAGTGGCAATATCTTGAT+TGG | 0.475261 | 4.4:+1882704 | None:intergenic |
| CTGTCTTCTTGCTTTCGTCT+CGG | 0.478922 | 4.4:+1880204 | None:intergenic |
| TTAGGTGATCACGGTGGCGA+AGG | 0.483199 | 4.4:-1886476 | MS.gene058550:CDS |
| GCGAAAATGAAGGTTCTTGA+AGG | 0.491969 | 4.4:-1882334 | MS.gene058550:intron |
| CTTCCTTCCGTCGCCGTCGT+CGG | 0.492244 | 4.4:-1886434 | MS.gene058550:CDS |
| AAGGAAAGATGGCGATCAAA+GGG | 0.493239 | 4.4:+1881504 | None:intergenic |
| GCATTCCGGTTTGAATTTGG+TGG | 0.498132 | 4.4:+1880092 | None:intergenic |
| CAGAGTTAGACCGCATTGGC+AGG | 0.501137 | 4.4:-1881920 | MS.gene058550:CDS |
| AATACAGGCTGCAGCATCCA+TGG | 0.504183 | 4.4:-1882306 | MS.gene058550:CDS |
| AGAGAAATCAAATAGCTAAG+AGG | 0.505782 | 4.4:-1878486 | MS.gene058550:CDS |
| GAACCAGAAAAGAACCCAAA+TGG | 0.505929 | 4.4:-1880116 | MS.gene058550:CDS |
| TCAGACGTCCTGGTGGGGAC+CGG | 0.509636 | 4.4:-1881575 | MS.gene058550:intron |
| CAGTAAAATTATCATTGTTG+GGG | 0.514716 | 4.4:+1880066 | None:intergenic |
| AGTCTTCTATAACCCTGCTC+TGG | 0.517134 | 4.4:+1881423 | None:intergenic |
| AACTTCTATTTCAGACGTCC+TGG | 0.519751 | 4.4:-1881585 | MS.gene058550:intron |
| CAAGAAGACAGTTACGCGGC+TGG | 0.521076 | 4.4:-1880192 | MS.gene058550:CDS |
| CCGTATATCCGGTCCCCACC+AGG | 0.521654 | 4.4:+1881567 | None:intergenic |
| TAAGGAAAGATGGCGATCAA+AGG | 0.525558 | 4.4:+1881503 | None:intergenic |
| CATCCACGGAGGCTTCTGCT+GGG | 0.526525 | 4.4:+1881370 | None:intergenic |
| GGAAGAGACTTTCTTCCTCG+TGG | 0.528185 | 4.4:-1886368 | MS.gene058550:CDS |
| ATGGACCACCAAATTCAAAC+CGG | 0.530820 | 4.4:-1880097 | MS.gene058550:CDS |
| AAAGTTGTCACTGAAGCTGA+TGG | 0.531731 | 4.4:-1881468 | MS.gene058550:CDS |
| GTGGCAATATCTTGATTGGC+GGG | 0.532340 | 4.4:+1882708 | None:intergenic |
| CATTGGCAGGCCGATTGCAG+TGG | 0.533531 | 4.4:-1881907 | MS.gene058550:CDS |
| GATACGCAGAAGGAGAAATT+GGG | 0.537412 | 4.4:-1878544 | MS.gene058550:intron |
| CTGCAATCGGCCTGCCAATG+CGG | 0.540716 | 4.4:+1881910 | None:intergenic |
| AGAAAGAGATTCACCGACTT+CGG | 0.540723 | 4.4:-1885591 | MS.gene058550:intron |
| TCAGCCCAGGAAGATCTATG+AGG | 0.540894 | 4.4:+1884267 | None:intergenic |
| GCGACGATGACGAGCTTGAT+CGG | 0.541831 | 4.4:-1886533 | MS.gene058550:CDS |
| ACGATATCGACTCTGTGGCT+TGG | 0.542824 | 4.4:-1878438 | MS.gene058550:CDS |
| AGGCTGCAGCATCCATGGGT+TGG | 0.551004 | 4.4:-1882301 | MS.gene058550:CDS |
| TGGGATCAGAATATCTTGCT+AGG | 0.551156 | 4.4:-1882162 | MS.gene058550:CDS |
| GGAGAAATTGGGTGCCATGT+TGG | 0.551512 | 4.4:-1878533 | MS.gene058550:CDS |
| CCTTCCGTCGCCGTCGTCGG+TGG | 0.552612 | 4.4:-1886431 | MS.gene058550:CDS |
| GAAACTTCAGTTACCGAAGT+CGG | 0.553424 | 4.4:+1885578 | None:intergenic |
| TGTTCAAGTTGGAAAGAAGG+AGG | 0.554198 | 4.4:-1879188 | MS.gene058550:intron |
| ACCGCACTTACCCTCCATCC+AGG | 0.555227 | 4.4:+1881718 | None:intergenic |
| GCCACCGTGATCACCTAAAA+CGG | 0.560290 | 4.4:+1886481 | None:intergenic |
| GGTTATAGAAGACTCATTGA+AGG | 0.563512 | 4.4:-1881414 | MS.gene058550:CDS |
| TATTGTTACGCGGAGGCCGC+TGG | 0.566066 | 4.4:-1885673 | MS.gene058550:CDS |
| TTTAAAGAGCACCTGGATGG+AGG | 0.566959 | 4.4:-1881729 | MS.gene058550:CDS |
| TATTAATATCAGCTTGTGAA+CGG | 0.567091 | 4.4:+1882269 | None:intergenic |
| TAAAGTATTTAAAGAGCACC+TGG | 0.567215 | 4.4:-1881736 | MS.gene058550:CDS |
| CGAAAATGAAGGTTCTTGAA+GGG | 0.572722 | 4.4:-1882333 | MS.gene058550:intron |
| CATGATTGTTGCTCGAAGGA+AGG | 0.574494 | 4.4:-1882236 | MS.gene058550:CDS |
| TCATAAGACAGAAAATGGCC+AGG | 0.574644 | 4.4:-1885640 | MS.gene058550:CDS |
| TCTAACTTGACAATGGACTA+CGG | 0.575000 | 4.4:+1879243 | None:intergenic |
| CTAAAACGGTACAAGCTCGT+TGG | 0.575495 | 4.4:+1886495 | None:intergenic |
| GGTCCGTTCGTATGTTGAGA+AGG | 0.575952 | 4.4:-1884077 | MS.gene058550:intron |
| TAACCCTCATAGATCTTCCT+GGG | 0.576713 | 4.4:-1884271 | MS.gene058550:CDS |
| GCAATGTCATTTGAAAGCGT+TGG | 0.577613 | 4.4:+1880257 | None:intergenic |
| TGGCTTCTCTAACTTGACAA+TGG | 0.578361 | 4.4:+1879236 | None:intergenic |
| AATGCAGAGTTAGACCGCAT+TGG | 0.578744 | 4.4:-1881924 | MS.gene058550:CDS |
| CGGTTCACAATTCCAACCCA+TGG | 0.581424 | 4.4:+1882289 | None:intergenic |
| CCACCGACGACGGCGACGGA+AGG | 0.582483 | 4.4:+1886431 | None:intergenic |
| CCATGCAGCTGCTGTGAGGA+AGG | 0.584586 | 4.4:-1885146 | MS.gene058550:intron |
| ATCCAGTGCTTCATTTGCAG+CGG | 0.584720 | 4.4:+1880232 | None:intergenic |
| ATGGCTAAGATAATGCAGTT+CGG | 0.585285 | 4.4:+1882735 | None:intergenic |
| AGTGGCAATATCTTGATTGG+CGG | 0.585927 | 4.4:+1882707 | None:intergenic |
| CTATTTCAGACGTCCTGGTG+GGG | 0.587055 | 4.4:-1881580 | MS.gene058550:intron |
| CTGTCCACCGACGACGGCGA+CGG | 0.587559 | 4.4:+1886427 | None:intergenic |
| TTCTATTTCAGACGTCCTGG+TGG | 0.588961 | 4.4:-1881582 | MS.gene058550:intron |
| CATCTGATTGCTCCAGAGCA+GGG | 0.591556 | 4.4:-1881435 | MS.gene058550:CDS |
| AGATCTTCCTGGGCTGACAA+AGG | 0.592661 | 4.4:-1884261 | MS.gene058550:CDS |
| TTAAAGAGCACCTGGATGGA+GGG | 0.592992 | 4.4:-1881728 | MS.gene058550:intron |
| CAAGAAGATTACAGCATCCA+CGG | 0.593135 | 4.4:+1881356 | None:intergenic |
| AACCGCTGCAAATGAAGCAC+TGG | 0.596471 | 4.4:-1880234 | MS.gene058550:CDS |
| AGTAAAATTATCATTGTTGG+GGG | 0.597465 | 4.4:+1880067 | None:intergenic |
| GCCGATTGCAGTGGATTCCG+GGG | 0.599749 | 4.4:-1881898 | MS.gene058550:intron |
| ACTGCAACCTTTGTCAGCCC+AGG | 0.602242 | 4.4:+1884254 | None:intergenic |
| GTATTTAAAGAGCACCTGGA+TGG | 0.604835 | 4.4:-1881732 | MS.gene058550:CDS |
| TCTATTTCAGACGTCCTGGT+GGG | 0.606106 | 4.4:-1881581 | MS.gene058550:intron |
| GGGCTGACAAAGGTTGCAGT+AGG | 0.607376 | 4.4:-1884251 | MS.gene058550:intron |
| AACAACACTTTCCAAGACAG+AGG | 0.607579 | 4.4:+1886391 | None:intergenic |
| TGCTCTGGAGCAATCAGATG+AGG | 0.607841 | 4.4:+1881438 | None:intergenic |
| GTTATAGAAGACTCATTGAA+GGG | 0.609274 | 4.4:-1881413 | MS.gene058550:CDS |
| ATACAGGCTGCAGCATCCAT+GGG | 0.610950 | 4.4:-1882305 | MS.gene058550:CDS |
| GGGTCTATCAGCTATTTCAA+AGG | 0.611791 | 4.4:-1881393 | MS.gene058550:CDS |
| AAGTTGTCACTGAAGCTGAT+GGG | 0.616414 | 4.4:-1881467 | MS.gene058550:CDS |
| CAGCCCAGGAAGATCTATGA+GGG | 0.621732 | 4.4:+1884268 | None:intergenic |
| AAAATTACGTACCAATCTTG+CGG | 0.622307 | 4.4:+1880033 | None:intergenic |
| AGGTGAAAGAACATTTGGAG+TGG | 0.629893 | 4.4:-1882514 | MS.gene058550:CDS |
| TCGACATGATTGTTGCTCGA+AGG | 0.630142 | 4.4:-1882240 | MS.gene058550:CDS |
| AGGCCCAGCAGAAGCCTCCG+TGG | 0.632406 | 4.4:-1881373 | MS.gene058550:CDS |
| TTACCTTCTCAACATACGAA+CGG | 0.640976 | 4.4:+1884074 | None:intergenic |
| GGATGGGTCTTCATCCAACA+TGG | 0.649372 | 4.4:+1878519 | None:intergenic |
| TTATGAAGTTGCAGTACCAG+CGG | 0.659990 | 4.4:+1885657 | None:intergenic |
| ATGAAACAGATCGAATAACT+GGG | 0.663724 | 4.4:-1885114 | MS.gene058550:CDS |
| TAAGTCAATTGCAGAAACAG+AGG | 0.664329 | 4.4:-1880656 | MS.gene058550:intron |
| ATTTACAAGGTATTGTTACG+CGG | 0.666912 | 4.4:-1885683 | MS.gene058550:intron |
| GTAAAATTATCATTGTTGGG+GGG | 0.668430 | 4.4:+1880068 | None:intergenic |
| AAATGAAGGTTCTTGAAGGG+CGG | 0.668784 | 4.4:-1882330 | MS.gene058550:intron |
| TACAAGGTATTGTTACGCGG+AGG | 0.681922 | 4.4:-1885680 | MS.gene058550:intron |
| TGAACTCTGTCCACCGACGA+CGG | 0.684385 | 4.4:+1886421 | None:intergenic |
| ATTACGTACCAATCTTGCGG+AGG | 0.697717 | 4.4:+1880036 | None:intergenic |
| TAGAGACGATATCGACTCTG+TGG | 0.698748 | 4.4:-1878443 | MS.gene058550:CDS |
| ATGCCCATGCAGCTGCTGTG+AGG | 0.716543 | 4.4:-1885150 | MS.gene058550:intron |
| GAAGATTACAGCATCCACGG+AGG | 0.775671 | 4.4:+1881359 | None:intergenic |
| AAAGCAAGAAGACAGTTACG+CGG | 0.786445 | 4.4:-1880196 | MS.gene058550:CDS |
| AAATGAATACCAGATCCACG+AGG | 0.794029 | 4.4:+1886353 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 1878432 | 1886558 | 1878432 | ID=MS.gene058550 |
| chr4.4 | mRNA | 1878432 | 1886558 | 1878432 | ID=MS.gene058550.t1;Parent=MS.gene058550 |
| chr4.4 | exon | 1886363 | 1886558 | 1886363 | ID=MS.gene058550.t1.exon1;Parent=MS.gene058550.t1 |
| chr4.4 | CDS | 1886363 | 1886558 | 1886363 | ID=cds.MS.gene058550.t1;Parent=MS.gene058550.t1 |
| chr4.4 | exon | 1885592 | 1885696 | 1885592 | ID=MS.gene058550.t1.exon2;Parent=MS.gene058550.t1 |
| chr4.4 | CDS | 1885592 | 1885696 | 1885592 | ID=cds.MS.gene058550.t1;Parent=MS.gene058550.t1 |
| chr4.4 | exon | 1885059 | 1885160 | 1885059 | ID=MS.gene058550.t1.exon3;Parent=MS.gene058550.t1 |
| chr4.4 | CDS | 1885059 | 1885160 | 1885059 | ID=cds.MS.gene058550.t1;Parent=MS.gene058550.t1 |
| chr4.4 | exon | 1884252 | 1884302 | 1884252 | ID=MS.gene058550.t1.exon4;Parent=MS.gene058550.t1 |
| chr4.4 | CDS | 1884252 | 1884302 | 1884252 | ID=cds.MS.gene058550.t1;Parent=MS.gene058550.t1 |
| chr4.4 | exon | 1884078 | 1884139 | 1884078 | ID=MS.gene058550.t1.exon5;Parent=MS.gene058550.t1 |
| chr4.4 | CDS | 1884078 | 1884139 | 1884078 | ID=cds.MS.gene058550.t1;Parent=MS.gene058550.t1 |
| chr4.4 | exon | 1882667 | 1882757 | 1882667 | ID=MS.gene058550.t1.exon6;Parent=MS.gene058550.t1 |
| chr4.4 | CDS | 1882667 | 1882757 | 1882667 | ID=cds.MS.gene058550.t1;Parent=MS.gene058550.t1 |
| chr4.4 | exon | 1882470 | 1882534 | 1882470 | ID=MS.gene058550.t1.exon7;Parent=MS.gene058550.t1 |
| chr4.4 | CDS | 1882470 | 1882534 | 1882470 | ID=cds.MS.gene058550.t1;Parent=MS.gene058550.t1 |
| chr4.4 | exon | 1882150 | 1882344 | 1882150 | ID=MS.gene058550.t1.exon8;Parent=MS.gene058550.t1 |
| chr4.4 | CDS | 1882150 | 1882344 | 1882150 | ID=cds.MS.gene058550.t1;Parent=MS.gene058550.t1 |
| chr4.4 | exon | 1881899 | 1882018 | 1881899 | ID=MS.gene058550.t1.exon9;Parent=MS.gene058550.t1 |
| chr4.4 | CDS | 1881899 | 1882018 | 1881899 | ID=cds.MS.gene058550.t1;Parent=MS.gene058550.t1 |
| chr4.4 | exon | 1881729 | 1881799 | 1881729 | ID=MS.gene058550.t1.exon10;Parent=MS.gene058550.t1 |
| chr4.4 | CDS | 1881729 | 1881799 | 1881729 | ID=cds.MS.gene058550.t1;Parent=MS.gene058550.t1 |
| chr4.4 | exon | 1881368 | 1881593 | 1881368 | ID=MS.gene058550.t1.exon11;Parent=MS.gene058550.t1 |
| chr4.4 | CDS | 1881368 | 1881593 | 1881368 | ID=cds.MS.gene058550.t1;Parent=MS.gene058550.t1 |
| chr4.4 | exon | 1880657 | 1880707 | 1880657 | ID=MS.gene058550.t1.exon12;Parent=MS.gene058550.t1 |
| chr4.4 | CDS | 1880657 | 1880707 | 1880657 | ID=cds.MS.gene058550.t1;Parent=MS.gene058550.t1 |
| chr4.4 | exon | 1880045 | 1880294 | 1880045 | ID=MS.gene058550.t1.exon13;Parent=MS.gene058550.t1 |
| chr4.4 | CDS | 1880045 | 1880294 | 1880045 | ID=cds.MS.gene058550.t1;Parent=MS.gene058550.t1 |
| chr4.4 | exon | 1879189 | 1879328 | 1879189 | ID=MS.gene058550.t1.exon14;Parent=MS.gene058550.t1 |
| chr4.4 | CDS | 1879189 | 1879328 | 1879189 | ID=cds.MS.gene058550.t1;Parent=MS.gene058550.t1 |
| chr4.4 | exon | 1878432 | 1878557 | 1878432 | ID=MS.gene058550.t1.exon15;Parent=MS.gene058550.t1 |
| chr4.4 | CDS | 1878432 | 1878557 | 1878432 | ID=cds.MS.gene058550.t1;Parent=MS.gene058550.t1 |
| Gene Sequence |
| Protein sequence |
