AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene40603

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene40603.t1 MTR_5g011620 81.379 290 50 1 8 297 3 288 4.19e-156 458
MS.gene40603.t1 MTR_5g011620 65.333 150 43 3 277 422 114 258 1.56e-49 179
MS.gene40603.t1 MTR_5g011610 68.919 296 79 3 87 372 11 303 3.26e-135 391
MS.gene40603.t1 MTR_5g011610 63.576 151 44 3 278 422 56 201 6.45e-51 174
MS.gene40603.t1 MTR_5g011680 89.055 201 17 2 229 425 93 292 1.25e-116 342
MS.gene40603.t1 MTR_5g011680 60.959 292 77 7 8 267 3 289 9.50e-102 305
MS.gene40603.t1 MTR_4g101990 46.316 285 113 7 11 265 17 291 5.71e-49 169
MS.gene40603.t1 MTR_4g101990 48.095 210 99 3 209 417 84 284 1.50e-39 144
MS.gene40603.t1 MTR_7g074935 46.014 276 113 8 10 270 28 282 1.10e-47 166
MS.gene40603.t1 MTR_7g074935 52.083 192 83 2 222 412 78 261 1.88e-42 152
MS.gene40603.t1 MTR_1g076710 44.215 242 91 6 51 262 49 276 4.94e-43 154
MS.gene40603.t1 MTR_1g076710 55.862 145 62 2 270 413 126 269 3.30e-33 127
MS.gene40603.t1 MTR_8g036160 44.589 231 79 5 51 254 71 279 1.71e-42 153
MS.gene40603.t1 MTR_8g036160 56.835 139 59 1 267 404 131 269 3.49e-37 139
MS.gene40603.t1 MTR_1g061530 37.281 228 122 4 50 262 64 285 8.22e-38 140
MS.gene40603.t1 MTR_1g061530 43.558 163 85 3 264 423 127 285 4.86e-35 133
MS.gene40603.t1 MTR_8g098390 37.500 240 135 5 49 278 95 329 3.71e-37 139
MS.gene40603.t1 MTR_8g098390 37.267 161 89 2 267 416 155 314 1.49e-28 115
MS.gene40603.t1 MTR_6g061670 40.758 211 115 4 51 253 51 259 1.10e-34 131
MS.gene40603.t1 MTR_6g061670 44.966 149 78 1 276 424 123 267 1.42e-28 114
MS.gene40603.t1 MTR_6g061670 40.278 216 114 5 51 253 51 264 2.29e-34 130
MS.gene40603.t1 MTR_6g061670 44.156 154 82 1 272 425 124 273 1.55e-28 114
MS.gene40603.t1 MTR_5g010330 38.756 209 107 5 35 235 54 249 6.83e-33 126
MS.gene40603.t1 MTR_5g010330 42.424 132 75 1 263 394 119 249 1.50e-27 112
MS.gene40603.t1 MTR_8g031100 38.528 231 110 6 49 259 87 305 9.44e-31 121
MS.gene40603.t1 MTR_8g031100 37.356 174 96 3 263 424 140 312 8.05e-25 104
MS.gene40603.t1 MTR_5g011660 78.261 69 15 0 1 69 1 69 1.80e-28 107
MS.gene40603.t1 MTR_5g011590 46.108 167 54 4 9 175 3 133 2.79e-28 108
MS.gene40603.t1 MTR_5g011590 55.072 69 29 1 267 335 73 139 8.03e-17 77.4
MS.gene40603.t1 MTR_4g098460 50.407 123 55 2 121 238 78 199 3.62e-28 112
MS.gene40603.t1 MTR_4g098460 46.154 117 62 1 278 394 81 196 5.79e-27 109
MS.gene40603.t1 MTR_7g116320 39.500 200 109 4 47 241 70 262 3.53e-27 112
MS.gene40603.t1 MTR_7g116320 48.718 117 57 1 278 394 143 256 3.55e-21 94.7
MS.gene40603.t1 MTR_7g077120 36.036 222 117 5 40 250 81 288 4.43e-27 111
MS.gene40603.t1 MTR_7g077120 38.776 147 90 0 263 409 142 288 1.80e-26 109
MS.gene40603.t1 MTR_7g116320 39.706 204 110 5 47 245 70 265 3.30e-26 107
MS.gene40603.t1 MTR_7g116320 47.788 113 56 1 278 390 143 252 7.70e-19 86.3
MS.gene40603.t1 MTR_5g011600 61.458 96 25 3 332 423 5 92 3.31e-26 102
MS.gene40603.t1 MTR_5g011600 58.065 93 36 1 178 270 5 94 2.86e-25 100
MS.gene40603.t1 MTR_4g098460 48.438 128 55 3 121 238 78 204 7.09e-26 106
MS.gene40603.t1 MTR_4g098460 44.262 122 62 2 278 394 81 201 1.09e-24 103
MS.gene40603.t1 MTR_1g079780 50.877 114 53 2 124 234 109 222 7.72e-26 107
MS.gene40603.t1 MTR_1g079780 44.715 123 63 3 278 398 109 228 1.60e-22 98.2
MS.gene40603.t1 MTR_1g038960 33.193 238 128 6 52 275 86 306 5.82e-24 102
MS.gene40603.t1 MTR_1g038960 43.548 124 65 3 272 393 143 263 3.93e-21 94.4
MS.gene40603.t1 MTR_4g055803 34.842 221 124 5 48 258 57 267 1.64e-23 102
MS.gene40603.t1 MTR_4g055803 37.241 145 88 2 263 404 110 254 1.72e-22 99.4
MS.gene40603.t1 MTR_5g014360 35.678 199 96 7 52 234 121 303 1.28e-22 99.4
MS.gene40603.t1 MTR_5g014360 40.171 117 66 2 278 393 190 303 1.59e-17 84.3
MS.gene40603.t1 MTR_4g055813 37.013 154 92 3 267 419 8 157 3.03e-22 96.3
MS.gene40603.t1 MTR_4g055813 41.912 136 75 3 127 258 22 157 1.05e-21 94.7
MS.gene40603.t1 MTR_4g055833 34.010 197 110 4 48 234 54 240 8.59e-22 96.3
MS.gene40603.t1 MTR_4g055833 35.862 145 90 2 263 404 107 251 9.22e-20 90.5
MS.gene40603.t1 MTR_6g060670 34.597 211 110 8 43 243 104 296 2.95e-19 88.2
MS.gene40603.t1 MTR_6g060670 41.667 132 72 4 271 402 170 296 9.03e-18 84.0
MS.gene40603.t1 MTR_5g013390 33.508 191 110 6 51 234 81 261 5.12e-19 88.2
MS.gene40603.t1 MTR_5g013390 35.593 118 72 2 277 393 147 261 1.84e-14 74.7
MS.gene40603.t1 MTR_3g116100 40.152 132 70 3 135 261 154 281 1.11e-17 84.0
MS.gene40603.t1 MTR_3g116100 41.803 122 64 3 282 400 144 261 1.12e-16 81.3
MS.gene40603.t1 MTR_1g038990 30.612 196 102 6 51 240 82 249 2.46e-16 80.1
MS.gene40603.t1 MTR_1g038990 35.043 117 71 3 278 393 131 243 1.11e-12 68.9
MS.gene40603.t1 MTR_3g100470 31.618 136 85 3 122 251 99 232 1.05e-15 77.8
MS.gene40603.t1 MTR_3g100470 30.469 128 88 1 278 404 101 228 5.65e-14 72.4
MS.gene40603.t1 MTR_4g055705 30.583 206 127 6 48 237 53 258 2.15e-15 77.0
MS.gene40603.t1 MTR_4g055705 34.247 146 88 3 250 392 114 254 1.52e-14 74.3
MS.gene40603.t1 MTR_5g041430 29.675 246 126 7 51 284 50 260 2.85e-15 76.6
MS.gene40603.t1 MTR_5g041430 31.293 147 95 4 263 408 97 238 2.15e-12 68.2
MS.gene40603.t1 MTR_5g011700 39.416 137 72 5 135 263 10 143 4.93e-15 73.6
MS.gene40603.t1 MTR_5g011700 40.566 106 59 2 289 393 10 112 1.44e-12 66.6
MS.gene40603.t1 MTR_3g068035 37.190 121 73 2 124 242 106 225 1.60e-14 74.3
MS.gene40603.t1 MTR_3g068035 35.398 113 70 1 281 393 109 218 6.28e-12 66.6
MS.gene40603.t1 MTR_3g023560 36.054 147 82 5 119 257 57 199 5.03e-14 72.0
MS.gene40603.t1 MTR_3g023560 34.188 117 71 2 280 393 64 177 2.59e-13 70.1
MS.gene40603.t1 MTR_7g080980 37.121 132 79 2 122 251 82 211 2.01e-13 70.5
MS.gene40603.t1 MTR_1g044155 34.959 123 77 2 122 242 151 272 3.07e-12 68.2
MS.gene40603.t1 MTR_5g066020 32.031 128 80 4 127 249 83 208 5.56e-12 65.9
MS.gene40603.t1 MTR_5g066020 29.299 157 105 4 258 410 60 214 5.67e-12 65.9
MS.gene40603.t1 MTR_4g098450 35.338 133 79 3 122 247 101 233 6.00e-11 63.5
MS.gene40603.t1 MTR_8g036060 35.294 136 83 3 124 257 113 245 8.52e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene40603.t1 AT4G12080 49.145 234 96 7 51 266 89 317 1.23e-57 193
MS.gene40603.t1 AT4G12080 53.988 163 71 2 263 421 155 317 1.54e-47 167
MS.gene40603.t1 AT5G62260 43.891 221 96 6 49 247 74 288 8.58e-46 163
MS.gene40603.t1 AT5G62260 50.365 137 68 0 270 406 152 288 1.24e-36 139
MS.gene40603.t1 AT5G62260 43.891 221 96 6 49 247 74 288 8.58e-46 163
MS.gene40603.t1 AT5G62260 50.365 137 68 0 270 406 152 288 1.24e-36 139
MS.gene40603.t1 AT4G25320 42.925 212 98 5 51 244 86 292 1.01e-43 157
MS.gene40603.t1 AT4G25320 52.448 143 66 1 261 401 147 289 3.21e-38 143
MS.gene40603.t1 AT5G51590 41.026 234 99 5 42 236 62 295 1.95e-43 157
MS.gene40603.t1 AT5G51590 56.452 124 54 0 271 394 171 294 6.51e-37 139
MS.gene40603.t1 AT5G62260 42.342 222 96 7 49 248 74 285 1.59e-42 151
MS.gene40603.t1 AT5G62260 48.800 125 64 0 270 394 152 276 2.43e-32 124
MS.gene40603.t1 AT4G22770 47.030 202 104 2 220 420 94 293 1.49e-39 145
MS.gene40603.t1 AT4G22770 42.609 230 106 6 49 259 70 292 1.91e-36 136
MS.gene40603.t1 AT4G22770 47.030 202 104 2 220 420 94 293 1.49e-39 145
MS.gene40603.t1 AT4G22770 42.609 230 106 6 49 259 70 292 1.91e-36 136
MS.gene40603.t1 AT2G33620 41.791 201 103 3 49 243 95 287 2.35e-39 145
MS.gene40603.t1 AT2G33620 50.694 144 68 1 262 402 144 287 1.04e-36 137
MS.gene40603.t1 AT2G33620 41.791 201 103 3 49 243 95 287 2.35e-39 145
MS.gene40603.t1 AT2G33620 50.694 144 68 1 262 402 144 287 1.04e-36 137
MS.gene40603.t1 AT2G33620 41.791 201 103 3 49 243 95 287 2.35e-39 145
MS.gene40603.t1 AT2G33620 50.694 144 68 1 262 402 144 287 1.04e-36 137
MS.gene40603.t1 AT2G33620 41.791 201 103 3 49 243 95 287 2.35e-39 145
MS.gene40603.t1 AT2G33620 50.694 144 68 1 262 402 144 287 1.04e-36 137
MS.gene40603.t1 AT4G00200 55.245 143 63 1 276 418 121 262 5.18e-37 137
MS.gene40603.t1 AT4G00200 44.196 224 103 6 41 250 40 255 1.43e-36 136
MS.gene40603.t1 AT4G00200 55.245 143 63 1 276 418 121 262 5.18e-37 137
MS.gene40603.t1 AT4G00200 44.196 224 103 6 41 250 40 255 1.43e-36 136
MS.gene40603.t1 AT2G45850 39.912 228 106 6 49 263 96 305 5.23e-36 135
MS.gene40603.t1 AT2G45850 42.177 147 82 1 263 409 147 290 1.36e-31 124
MS.gene40603.t1 AT2G45850 39.912 228 106 6 49 263 96 305 5.23e-36 135
MS.gene40603.t1 AT2G45850 42.177 147 82 1 263 409 147 290 1.36e-31 124
MS.gene40603.t1 AT2G45850 40.201 199 89 4 49 234 96 277 4.18e-33 126
MS.gene40603.t1 AT2G45850 42.748 131 75 0 263 393 147 277 2.09e-28 113
MS.gene40603.t1 AT1G63470 41.146 192 102 4 51 235 105 292 2.53e-32 126
MS.gene40603.t1 AT1G63470 41.667 132 76 1 263 394 162 292 9.80e-27 110
MS.gene40603.t1 AT4G17950 37.327 217 112 5 51 243 131 347 1.01e-31 125
MS.gene40603.t1 AT4G17950 44.000 150 79 3 278 422 221 370 1.12e-28 117
MS.gene40603.t1 AT3G61310 39.474 228 96 7 51 261 101 303 2.49e-31 123
MS.gene40603.t1 AT3G61310 40.909 132 78 0 263 394 149 280 1.24e-24 104
MS.gene40603.t1 AT5G46640 41.256 223 115 5 27 236 78 297 5.06e-30 120
MS.gene40603.t1 AT5G46640 42.857 161 85 2 262 415 162 322 1.59e-23 102
MS.gene40603.t1 AT3G04590 35.683 227 135 4 47 271 101 318 4.18e-29 118
MS.gene40603.t1 AT3G04590 45.690 116 60 1 278 393 171 283 3.66e-24 103
MS.gene40603.t1 AT3G04590 36.410 195 115 2 49 241 103 290 8.28e-29 115
MS.gene40603.t1 AT3G04590 45.690 116 60 1 278 393 171 283 9.53e-25 103
MS.gene40603.t1 AT1G63480 35.366 246 108 8 51 268 93 315 7.11e-28 113
MS.gene40603.t1 AT1G63480 38.356 146 85 2 263 408 139 279 2.03e-24 103
MS.gene40603.t1 AT1G63480 35.366 246 108 8 51 268 93 315 7.11e-28 113
MS.gene40603.t1 AT1G63480 38.356 146 85 2 263 408 139 279 2.03e-24 103
MS.gene40603.t1 AT1G63480 35.366 246 108 8 51 268 93 315 7.11e-28 113
MS.gene40603.t1 AT1G63480 38.356 146 85 2 263 408 139 279 2.03e-24 103
MS.gene40603.t1 AT1G63480 35.366 246 108 8 51 268 93 315 7.11e-28 113
MS.gene40603.t1 AT1G63480 38.356 146 85 2 263 408 139 279 2.03e-24 103
MS.gene40603.t1 AT1G63480 35.366 246 108 8 51 268 93 315 7.11e-28 113
MS.gene40603.t1 AT1G63480 38.356 146 85 2 263 408 139 279 2.03e-24 103
MS.gene40603.t1 AT1G63480 35.366 246 108 8 51 268 99 321 7.82e-28 113
MS.gene40603.t1 AT1G63480 38.356 146 85 2 263 408 145 285 2.09e-24 103
MS.gene40603.t1 AT1G63480 35.366 246 108 8 51 268 99 321 7.82e-28 113
MS.gene40603.t1 AT1G63480 38.356 146 85 2 263 408 145 285 2.09e-24 103
MS.gene40603.t1 AT2G36560 44.167 120 65 2 278 395 105 224 3.96e-21 96.3
MS.gene40603.t1 AT2G36560 39.344 122 67 3 121 235 102 223 1.27e-17 85.5
MS.gene40603.t1 AT5G49700 34.815 135 77 3 128 253 87 219 2.25e-17 82.4
MS.gene40603.t1 AT5G49700 32.168 143 85 4 278 409 83 224 4.57e-15 75.5
MS.gene40603.t1 AT3G60870 33.628 113 71 2 124 234 86 196 1.01e-14 74.3
MS.gene40603.t1 AT3G60870 28.834 163 104 4 231 393 46 196 7.30e-13 68.9
MS.gene40603.t1 AT1G14490 34.848 132 75 3 128 250 34 163 5.22e-14 71.2
MS.gene40603.t1 AT1G14490 31.293 147 90 3 279 414 31 177 1.11e-12 67.4
MS.gene40603.t1 AT1G14490 34.848 132 75 3 128 250 53 182 5.28e-14 71.6
MS.gene40603.t1 AT1G14490 30.405 148 92 3 273 409 44 191 1.51e-12 67.4
MS.gene40603.t1 AT2G42940 33.113 151 95 3 105 253 63 209 4.02e-13 69.7
MS.gene40603.t1 AT3G04570 30.818 159 88 4 98 234 69 227 1.90e-11 65.1
MS.gene40603.t1 AT2G35270 35.385 130 80 2 123 250 104 231 2.09e-11 64.7
MS.gene40603.t1 AT4G17800 34.615 130 81 2 123 250 108 235 5.20e-11 63.5

Find 108 sgRNAs with CRISPR-Local

Find 191 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCCGTCTGGTCCATACTTTC+TGG 0.172462 5.1:-5136067 None:intergenic
TTCCAGGTACTTACATTTAC+AGG 0.203699 5.1:-5137182 None:intergenic
AAGATAGGCTGTTAGGTATT+TGG 0.208183 5.1:-5138907 None:intergenic
TGGATTCTCCAACTCGAATT+TGG 0.209769 5.1:-5136145 None:intergenic
CGCATTATCACAGTTAATTC+TGG 0.217530 5.1:+5136614 MS.gene40603:CDS
TATGAAATTCGAACTTTATC+TGG 0.233802 5.1:+5138402 MS.gene40603:CDS
TATGAAATTATATCTTTATC+TGG 0.243485 5.1:+5137050 MS.gene40603:CDS
GACCAGGTACTTACATTTAC+AGG 0.268526 5.1:-5138549 None:intergenic
ATTCCAGTTCTTGGTGCTGC+AGG 0.307459 5.1:-5135946 None:intergenic
CGCGGAAGGCGGAGTATATC+TGG 0.344559 5.1:+5138447 MS.gene40603:CDS
AGGGGTCGCCCCAGAAAGTA+TGG 0.356183 5.1:+5136057 MS.gene40603:CDS
CCGTCTGGTCCATACTTTCT+GGG 0.367431 5.1:-5136066 None:intergenic
TGTTGAGTTTGATCTTCAAT+AGG 0.372062 5.1:-5136542 None:intergenic
TGGCATGAGTGTTTCTTTAG+TGG 0.383981 5.1:+5138470 MS.gene40603:CDS
AAAGCTGGGATTCCAGTTCT+TGG 0.386982 5.1:-5135955 None:intergenic
GATGTAAAAGTAGTGCCTTC+TGG 0.395117 5.1:-5136590 None:intergenic
GTTGAGTTTGATCTTCAATA+GGG 0.398662 5.1:-5136541 None:intergenic
TTCTGCTCATGTTGTTCACT+TGG 0.400794 5.1:-5138823 None:intergenic
GTTTCTTTAGCGGGTCTAGA+TGG 0.404137 5.1:+5137113 MS.gene40603:CDS
ATCGTTGGAGGTTGCGTAGC+TGG 0.408342 5.1:+5137140 MS.gene40603:CDS
ACCAGACGGGTCTTTAATCT+TGG 0.410529 5.1:+5136080 MS.gene40603:CDS
GATAGAATGCATATAGCTTC+TGG 0.411982 5.1:-5136816 None:intergenic
CCACCACTGCGACTTTCGTT+TGG 0.412733 5.1:-5137083 None:intergenic
GATAGAATGCATATAGCTTC+TGG 0.414422 5.1:-5138153 None:intergenic
GCAGTAGTCATGGGAGCAGC+TGG 0.419326 5.1:-5138880 None:intergenic
AACCCTGCAGCACCAAGAAC+TGG 0.424961 5.1:+5135943 MS.gene40603:CDS
GTGGGTCCATCGGCAAAAGC+TGG 0.432662 5.1:-5135970 None:intergenic
CCTTATGCTCTAGTTCTTGA+GGG 0.436524 5.1:-5136565 None:intergenic
CACAGCTGATTCGACATCTC+AGG 0.441577 5.1:+5135990 MS.gene40603:CDS
TGGCGCCAGCAAAGAGGAAA+AGG 0.445228 5.1:+5136037 MS.gene40603:CDS
GGTATGAGTGTTTCTTTAGC+GGG 0.447972 5.1:+5137104 MS.gene40603:CDS
GTTGTTGGCGGTCGTGTAGC+TGG 0.449009 5.1:+5138507 MS.gene40603:CDS
TAACTGAGCTTGCAAGCAAT+TGG 0.450726 5.1:-5137835 None:intergenic
GGCGCCACCATTTGCAGCGG+TGG 0.453790 5.1:-5136021 None:intergenic
AGGCATTCCACCGCTGCAAA+TGG 0.457742 5.1:+5136014 MS.gene40603:CDS
ACCTTATGCTCTAGTTCTTG+AGG 0.457940 5.1:-5136566 None:intergenic
ATCTGGTTTGTTCATGCCAA+AGG 0.459811 5.1:+5138419 MS.gene40603:CDS
ACTCGAATTTGGCAGCTTTG+CGG 0.460198 5.1:-5136134 None:intergenic
CCCTCAAGAACTAGAGCATA+AGG 0.474150 5.1:+5136565 MS.gene40603:CDS
GGCGCCAGCAAAGAGGAAAA+GGG 0.481838 5.1:+5136038 MS.gene40603:CDS
GCTGCTCCCATGACTACTGC+TGG 0.488722 5.1:+5138883 MS.gene40603:CDS
ATATGCATTCTATCTGCAAA+TGG 0.495626 5.1:+5136825 MS.gene40603:CDS
ATGGAGGGTGCTAGCAATAG+TGG 0.502183 5.1:+5135898 MS.gene40603:CDS
GGTGCTGCAGGGTTATCTGT+TGG 0.503393 5.1:-5135934 None:intergenic
TTCCAGTTCTTGGTGCTGCA+GGG 0.504119 5.1:-5135945 None:intergenic
ATGGAGCTAGATGTTGGCTT+GGG 0.508152 5.1:-5136111 None:intergenic
TTGCGGATGGAGCTAGATGT+TGG 0.510588 5.1:-5136117 None:intergenic
TCGAACATTATTCACGTATG+AGG 0.511504 5.1:+5138224 MS.gene40603:CDS
GATGCTCCCGTGTCTACTGC+TGG 0.518304 5.1:+5137540 MS.gene40603:CDS
ATATGCATTCTATCTGTGAA+TGG 0.521611 5.1:+5138162 MS.gene40603:CDS
AGACGATTGAGGCTGACTAA+TGG 0.522248 5.1:-5136865 None:intergenic
AATAATGTTCGAGACGATTG+AGG 0.525993 5.1:-5138213 None:intergenic
CTGAGATGTCGAATCAGCTG+TGG 0.534004 5.1:-5135989 None:intergenic
GATGGAGCTAGATGTTGGCT+TGG 0.534927 5.1:-5136112 None:intergenic
GTTTCTTTAGTGGATCTACA+TGG 0.535434 5.1:+5138480 MS.gene40603:CDS
GCATTCACAATGAAAATATG+AGG 0.537005 5.1:-5137872 None:intergenic
AGAATTAACTGTGATAATGC+GGG 0.538033 5.1:-5136612 None:intergenic
TGGTATGAGTGTTTCTTTAG+CGG 0.540533 5.1:+5137103 MS.gene40603:CDS
ACAGGACTTGCAGCCACCAA+TGG 0.541015 5.1:-5138531 None:intergenic
CGAATCAGCTGTGGGTCCAT+CGG 0.541484 5.1:-5135980 None:intergenic
CATTCCACCGCTGCAAATGG+TGG 0.541527 5.1:+5136017 MS.gene40603:CDS
CGGTCGTGTAGCTGGTCCAT+TGG 0.545745 5.1:+5138515 MS.gene40603:CDS
ATTTGGACCAGCAGTAGTCA+TGG 0.546435 5.1:-5138890 None:intergenic
GAAATGCTTCACCTCATATG+TGG 0.547537 5.1:-5136898 None:intergenic
ACAGGACTTGCAGCTACCAA+TGG 0.547952 5.1:-5137164 None:intergenic
GAATTTGGCAGCTTTGCGGA+TGG 0.551187 5.1:-5136130 None:intergenic
TCGTGTAGCTGGTCCATTGG+TGG 0.554685 5.1:+5138518 MS.gene40603:CDS
GGTCTAGATGGACATATCGT+TGG 0.556357 5.1:+5137125 MS.gene40603:CDS
TGCCAAAGGAAAAGCGCGGA+AGG 0.557420 5.1:+5138433 MS.gene40603:CDS
TGGGTCCATCGGCAAAAGCT+GGG 0.559063 5.1:-5135969 None:intergenic
GCCAAGATTAAAGACCCGTC+TGG 0.560561 5.1:-5136081 None:intergenic
CAAAGCTGCCAAATTCGAGT+TGG 0.564111 5.1:+5136137 MS.gene40603:CDS
CAGAATTAACTGTGATAATG+CGG 0.564469 5.1:-5136613 None:intergenic
GATCTACATGGACATGTTGT+TGG 0.565136 5.1:+5138492 MS.gene40603:CDS
CTAGATGGACATATCGTTGG+AGG 0.567496 5.1:+5137128 MS.gene40603:CDS
GACACGGGAGCATCTGCCGT+TGG 0.568071 5.1:-5137531 None:intergenic
TTTCATTGTGAATGCTGGAG+AGG 0.570869 5.1:+5137880 MS.gene40603:CDS
CCCAGAAAGTATGGACCAGA+CGG 0.575081 5.1:+5136066 MS.gene40603:CDS
AGGTTGCGTAGCTGGTCCAT+TGG 0.577220 5.1:+5137148 MS.gene40603:CDS
GCTGGCGCCACCATTTGCAG+CGG 0.582574 5.1:-5136024 None:intergenic
GAGCTAGATGTTGGCTTGGG+AGG 0.595395 5.1:-5136108 None:intergenic
AAATTCGAGTTGGAGAATCC+AGG 0.596763 5.1:+5136147 MS.gene40603:CDS
CTACATGGACATGTTGTTGG+CGG 0.596833 5.1:+5138495 MS.gene40603:CDS
GACTTCAACGTCAATGCCAA+CGG 0.603268 5.1:+5137515 MS.gene40603:CDS
GATATGTATCAGGTAGTGGT+AGG 0.603294 5.1:+5138790 MS.gene40603:intron
TATCAAAAGCAGTCATTCGT+CGG 0.605711 5.1:+5138190 MS.gene40603:CDS
CTAGAGCATAAGGTGCCAGA+AGG 0.609617 5.1:+5136575 MS.gene40603:CDS
TATTGTACCAGCAGTAGACA+CGG 0.609633 5.1:-5137547 None:intergenic
AGTAAATTTAGATCCTCATG+AGG 0.610048 5.1:+5136517 MS.gene40603:CDS
TTTGGACCAGCAGTAGTCAT+GGG 0.613568 5.1:-5138889 None:intergenic
CGTCTGGTCCATACTTTCTG+GGG 0.621397 5.1:-5136065 None:intergenic
CCAAACGAAAGTCGCAGTGG+TGG 0.623451 5.1:+5137083 MS.gene40603:CDS
ACAGCTGATTCGACATCTCA+GGG 0.628768 5.1:+5135991 MS.gene40603:CDS
GCGCCAGCAAAGAGGAAAAG+GGG 0.629697 5.1:+5136039 MS.gene40603:CDS
TGAGATGTCGAATCAGCTGT+GGG 0.630120 5.1:-5135988 None:intergenic
TATCACAGTTAATTCTGGAG+AGG 0.631106 5.1:+5136619 MS.gene40603:CDS
AAATGGTGGCGCCAGCAAAG+AGG 0.640999 5.1:+5136031 MS.gene40603:CDS
CAAAGGAAAAGCGCGGAAGG+CGG 0.642169 5.1:+5138436 MS.gene40603:CDS
CGATTGAGGCTGACTAATGG+TGG 0.644722 5.1:-5136862 None:intergenic
CCAGAAAGTATGGACCAGAC+GGG 0.646931 5.1:+5136067 MS.gene40603:CDS
GTAAATTTAGATCCTCATGA+GGG 0.647623 5.1:+5136518 MS.gene40603:CDS
TCTTCAATAGGGCCCTCATG+AGG 0.663045 5.1:-5136530 None:intergenic
ACTCTAGTGAAGTTCATGGA+GGG 0.668635 5.1:+5135883 None:intergenic
ATTGTACCAGCAGTAGACAC+GGG 0.698737 5.1:-5137546 None:intergenic
GTGCCAAACGAAAGTCGCAG+TGG 0.704907 5.1:+5137080 MS.gene40603:CDS
GGAAGGCGGAGTATATCTGG+TGG 0.708998 5.1:+5138450 MS.gene40603:CDS
TTCATGCCAAAGGAAAAGCG+CGG 0.727437 5.1:+5138429 MS.gene40603:CDS
GCTGATTCGACATCTCAGGG+AGG 0.790272 5.1:+5135994 MS.gene40603:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTAATATATATCAAGTTAT+TGG + chr5.1:5137698-5137717 MS.gene40603:intron 10.0%
!! TATCTAATTATGAATATTAT+GGG - chr5.1:5137950-5137969 None:intergenic 10.0%
!! TTATCTAATTATGAATATTA+TGG - chr5.1:5137951-5137970 None:intergenic 10.0%
!! TATGAAATTATATCTTTATC+TGG + chr5.1:5137050-5137069 MS.gene40603:CDS 15.0%
!! TTAATATATATCAAGTGTAT+TGG + chr5.1:5136285-5136304 MS.gene40603:intron 15.0%
!!! TATATCTTGATATTTTTAAC+TGG + chr5.1:5137978-5137997 MS.gene40603:intron 15.0%
!! AATAATGCAAATTGAATTTC+AGG + chr5.1:5138374-5138393 MS.gene40603:intron 20.0%
!! AATAATGTCAATTGAATTTC+AGG + chr5.1:5137022-5137041 MS.gene40603:intron 20.0%
!! AATATATATCAAGTTATTGG+TGG + chr5.1:5137701-5137720 MS.gene40603:intron 20.0%
!! ATAATGCAAATTGAATTTCA+GGG + chr5.1:5138375-5138394 MS.gene40603:intron 20.0%
!! ATAATGTCAATTGAATTTCA+GGG + chr5.1:5137023-5137042 MS.gene40603:intron 20.0%
!! ATATCTTCGATATTCATAAT+TGG + chr5.1:5136644-5136663 MS.gene40603:intron 20.0%
!! TAAGATATAAGTGTAAATGT+TGG + chr5.1:5137738-5137757 MS.gene40603:intron 20.0%
!! TAAGATGTAATTGTAAATGT+TGG + chr5.1:5136326-5136345 MS.gene40603:intron 20.0%
!! TAATCTGATAGTAGTATTAT+TGG + chr5.1:5136923-5136942 MS.gene40603:intron 20.0%
!! TAATGACTGATTAGAATAAA+TGG + chr5.1:5136727-5136746 MS.gene40603:intron 20.0%
!!! TATTGGTATCAGTTTTAATA+TGG + chr5.1:5136940-5136959 MS.gene40603:intron 20.0%
! ATATATATCAAGTGTATTGG+TGG + chr5.1:5136288-5136307 MS.gene40603:intron 25.0%
! ATGCAATTCTGATAATTTAG+TGG + chr5.1:5138712-5138731 MS.gene40603:intron 25.0%
! GCTTATGTAGTTATTTATCT+TGG + chr5.1:5138618-5138637 MS.gene40603:intron 25.0%
! TATGAAATTCGAACTTTATC+TGG + chr5.1:5138402-5138421 MS.gene40603:CDS 25.0%
! TCAAAAATTATCCACATATG+AGG + chr5.1:5136887-5136906 MS.gene40603:CDS 25.0%
! TGATGACTAATCAGAATAAA+TGG + chr5.1:5138064-5138083 MS.gene40603:intron 25.0%
! TGTTCATAATTTCCTGATTT+CGG + chr5.1:5136397-5136416 MS.gene40603:intron 25.0%
! TTAAAGAAACACATCAAAAC+AGG - chr5.1:5137003-5137022 None:intergenic 25.0%
!!! GAATAGTGTATTTTGTTGTT+TGG + chr5.1:5137657-5137676 MS.gene40603:intron 25.0%
!!! TCAGTTTTAATATGGAGTTT+AGG + chr5.1:5136948-5136967 MS.gene40603:intron 25.0%
AATGTACTAATGTGGAGTTT+AGG + chr5.1:5138302-5138321 MS.gene40603:intron 30.0%
AGTAAATTTAGATCCTCATG+AGG + chr5.1:5136517-5136536 MS.gene40603:CDS 30.0%
ATACTAAAACACCAATTAGC+AGG + chr5.1:5137235-5137254 MS.gene40603:intron 30.0%
ATATGCATTCTATCTGCAAA+TGG + chr5.1:5136825-5136844 MS.gene40603:CDS 30.0%
ATATGCATTCTATCTGTGAA+TGG + chr5.1:5138162-5138181 MS.gene40603:CDS 30.0%
ATTCTCATTACTCTTTCCAA+TGG + chr5.1:5137409-5137428 MS.gene40603:intron 30.0%
CAACTGAATTTGCAAGAAAT+TGG - chr5.1:5136490-5136509 None:intergenic 30.0%
CAATTTCTTGCAAATTCAGT+TGG + chr5.1:5136488-5136507 MS.gene40603:intron 30.0%
CCACATTAGTACATTGAATT+AGG - chr5.1:5138297-5138316 None:intergenic 30.0%
CCTAATTCAATGTACTAATG+TGG + chr5.1:5138294-5138313 MS.gene40603:intron 30.0%
GCATTCACAATGAAAATATG+AGG - chr5.1:5137875-5137894 None:intergenic 30.0%
GCTAAAAACACAGAATAATG+AGG + chr5.1:5138846-5138865 MS.gene40603:CDS 30.0%
GTAAATTTAGATCCTCATGA+GGG + chr5.1:5136518-5136537 MS.gene40603:CDS 30.0%
GTTGAGTTTGATCTTCAATA+GGG - chr5.1:5136544-5136563 None:intergenic 30.0%
TAAATGTAAGTACCTGGAAA+GGG + chr5.1:5137186-5137205 MS.gene40603:intron 30.0%
TGAATAACAAACTACTCCTA+AGG - chr5.1:5138329-5138348 None:intergenic 30.0%
TGTTGAGTTTGATCTTCAAT+AGG - chr5.1:5136545-5136564 None:intergenic 30.0%
! AGAATTAACTGTGATAATGC+GGG - chr5.1:5136615-5136634 None:intergenic 30.0%
! CAGAATTAACTGTGATAATG+CGG - chr5.1:5136616-5136635 None:intergenic 30.0%
! CATATTTTCATTGTGAATGC+TGG + chr5.1:5137875-5137894 MS.gene40603:CDS 30.0%
! GTTCTTTCTTTCTTTTTGTC+TGG + chr5.1:5136464-5136483 MS.gene40603:intron 30.0%
!!! AAGTGCTTTCTTTTTTTCTC+AGG + chr5.1:5137812-5137831 MS.gene40603:intron 30.0%
!!! ATAATGTCATTTTGTCAGCA+AGG + chr5.1:5138129-5138148 MS.gene40603:CDS 30.0%
!!! GATAATTTTTGAGACGATTG+AGG - chr5.1:5136879-5136898 None:intergenic 30.0%
!!! GGAGAGGTAATATCATTTTT+TGG + chr5.1:5137896-5137915 MS.gene40603:intron 30.0%
AAGAGAATTTCTTAGCTACC+TGG - chr5.1:5136168-5136187 None:intergenic 35.0%
AATAATGTTCGAGACGATTG+AGG - chr5.1:5138216-5138235 None:intergenic 35.0%
AGATATTCAATGCCGAAATC+AGG - chr5.1:5136412-5136431 None:intergenic 35.0%
AGTCATCATTCCAGTGATAA+CGG - chr5.1:5138052-5138071 None:intergenic 35.0%
ATAGTAGCAATTTCGTTAGC+AGG - chr5.1:5137331-5137350 None:intergenic 35.0%
ATTCTCATCACTGTTTCCAA+TGG + chr5.1:5138764-5138783 MS.gene40603:intron 35.0%
CCTGATACATATCAATCCAT+TGG - chr5.1:5137428-5137447 None:intergenic 35.0%
CGCATTATCACAGTTAATTC+TGG + chr5.1:5136614-5136633 MS.gene40603:CDS 35.0%
GATAGAATGCATATAGCTTC+TGG - chr5.1:5136819-5136838 None:intergenic 35.0%
GATTGATATGTATCAGGTAG+TGG + chr5.1:5137431-5137450 MS.gene40603:intron 35.0%
GATTTAAGCATCCTGCTAAT+TGG - chr5.1:5137249-5137268 None:intergenic 35.0%
GTAAATGTAAGTACCTGGAA+AGG + chr5.1:5137185-5137204 MS.gene40603:intron 35.0%
GTAGTAAAACGCAAACTTTG+TGG + chr5.1:5137357-5137376 MS.gene40603:intron 35.0%
GTATTTGTTTCCGTTATCAC+TGG + chr5.1:5138039-5138058 MS.gene40603:intron 35.0%
TACCTGGAAAGGGAATAATA+GGG + chr5.1:5137196-5137215 MS.gene40603:intron 35.0%
TATCAAAAGCAGTCATTCGT+CGG + chr5.1:5138190-5138209 MS.gene40603:CDS 35.0%
TCGAACATTATTCACGTATG+AGG + chr5.1:5138224-5138243 MS.gene40603:CDS 35.0%
TCTGTTTCCTGCTAAAGAAA+TGG + chr5.1:5138671-5138690 MS.gene40603:intron 35.0%
TGGTATGAGTGTTTCTTTAG+CGG + chr5.1:5137103-5137122 MS.gene40603:CDS 35.0%
TTCCAGGTACTTACATTTAC+AGG - chr5.1:5137185-5137204 None:intergenic 35.0%
! AATGGCTACTACTTGTCTTT+TGG + chr5.1:5136745-5136764 MS.gene40603:intron 35.0%
! ATAGTTGCCATTTCTTTAGC+AGG - chr5.1:5138681-5138700 None:intergenic 35.0%
! GTTTCTTTAGTGGATCTACA+TGG + chr5.1:5138480-5138499 MS.gene40603:CDS 35.0%
! TATCACAGTTAATTCTGGAG+AGG + chr5.1:5136619-5136638 MS.gene40603:CDS 35.0%
! TTCGGCATTGAATATCTTTG+TGG + chr5.1:5136415-5136434 MS.gene40603:intron 35.0%
!! ACTTGTCTTTTGATTGTCTC+AGG + chr5.1:5138092-5138111 MS.gene40603:intron 35.0%
!! CCAATGGATTGATATGTATC+AGG + chr5.1:5137425-5137444 MS.gene40603:intron 35.0%
!! GAATAGTGCACTTTGTTGTT+CGG + chr5.1:5136243-5136262 MS.gene40603:intron 35.0%
!!! GTAATGTCATTTTGTCAGCA+AGG + chr5.1:5136792-5136811 MS.gene40603:CDS 35.0%
AAAAGACATATTCCCTAGCC+AGG - chr5.1:5138574-5138593 None:intergenic 40.0%
AAGAAAGAGAATCATCTCCG+TGG - chr5.1:5137598-5137617 None:intergenic 40.0%
ACGAAATTGCTACTATCCAG+TGG + chr5.1:5137335-5137354 MS.gene40603:intron 40.0%
GAAATGCTTCACCTCATATG+TGG - chr5.1:5136901-5136920 None:intergenic 40.0%
GACCAGGTACTTACATTTAC+AGG - chr5.1:5138552-5138571 None:intergenic 40.0%
GATCTACATGGACATGTTGT+TGG + chr5.1:5138492-5138511 MS.gene40603:CDS 40.0%
GATGTAAAAGTAGTGCCTTC+TGG - chr5.1:5136593-5136612 None:intergenic 40.0%
GCTAAAAACGCAGGAAAATG+AGG + chr5.1:5137491-5137510 MS.gene40603:CDS 40.0%
GGAAGATGAAGATGAGAGTT+AGG - chr5.1:5137573-5137592 None:intergenic 40.0%
GGTATGAGTGTTTCTTTAGC+GGG + chr5.1:5137104-5137123 MS.gene40603:CDS 40.0%
GTACCTGGAAAGGGAATAAT+AGG + chr5.1:5137195-5137214 MS.gene40603:intron 40.0%
GTCCTGTAAATGTAAGTACC+TGG + chr5.1:5137180-5137199 MS.gene40603:intron 40.0%
TAAATGTAAGTACCTGGTCC+TGG + chr5.1:5138553-5138572 MS.gene40603:intron 40.0%
TAACTGAGCTTGCAAGCAAT+TGG - chr5.1:5137838-5137857 None:intergenic 40.0%
TATTGTACCAGCAGTAGACA+CGG - chr5.1:5137550-5137569 None:intergenic 40.0%
TCATCTTCATCTTCCTTCCA+CGG + chr5.1:5137578-5137597 MS.gene40603:intron 40.0%
TGGATTCTCCAACTCGAATT+TGG - chr5.1:5136148-5136167 None:intergenic 40.0%
TGGCATGAGTGTTTCTTTAG+TGG + chr5.1:5138470-5138489 MS.gene40603:CDS 40.0%
TTCGCAAACTAAAGTCTAGC+AGG + chr5.1:5138593-5138612 MS.gene40603:intron 40.0%
TTCTGCTCATGTTGTTCACT+TGG - chr5.1:5138826-5138845 None:intergenic 40.0%
TTGTGATCATGCGAGTTTCA+TGG + chr5.1:5137617-5137636 MS.gene40603:intron 40.0%
TTTCATTGTGAATGCTGGAG+AGG + chr5.1:5137880-5137899 MS.gene40603:CDS 40.0%
! AATGTGGAGTTTAGGACCTT+AGG + chr5.1:5138310-5138329 MS.gene40603:intron 40.0%
! AGTTTGCGTTTTACTACCAC+TGG - chr5.1:5137354-5137373 None:intergenic 40.0%
! GATATGTATCAGGTAGTGGT+AGG + chr5.1:5137435-5137454 MS.gene40603:intron 40.0%
!! AAATTCGAGTTGGAGAATCC+AGG + chr5.1:5136147-5136166 MS.gene40603:CDS 40.0%
!! ACCTTATGCTCTAGTTCTTG+AGG - chr5.1:5136569-5136588 None:intergenic 40.0%
!! ATCTGGTTTGTTCATGCCAA+AGG + chr5.1:5138419-5138438 MS.gene40603:CDS 40.0%
!! CCTTATGCTCTAGTTCTTGA+GGG - chr5.1:5136568-5136587 None:intergenic 40.0%
AAAGCTGGGATTCCAGTTCT+TGG - chr5.1:5135958-5135977 None:intergenic 45.0%
AAGAGAATCATCTCCGTGGA+AGG - chr5.1:5137594-5137613 None:intergenic 45.0%
ACCAGACGGGTCTTTAATCT+TGG + chr5.1:5136080-5136099 MS.gene40603:CDS 45.0%
ACTCGAATTTGGCAGCTTTG+CGG - chr5.1:5136137-5136156 None:intergenic 45.0%
AGACGATTGAGGCTGACTAA+TGG - chr5.1:5136868-5136887 None:intergenic 45.0%
AGCCCTATTATTCCCTTTCC+AGG - chr5.1:5137201-5137220 None:intergenic 45.0%
ATTGTACCAGCAGTAGACAC+GGG - chr5.1:5137549-5137568 None:intergenic 45.0%
ATTTGGACCAGCAGTAGTCA+TGG - chr5.1:5138893-5138912 None:intergenic 45.0%
CAAAGCTGCCAAATTCGAGT+TGG + chr5.1:5136137-5136156 MS.gene40603:CDS 45.0%
CCCTCAAGAACTAGAGCATA+AGG + chr5.1:5136565-5136584 MS.gene40603:CDS 45.0%
CTACATGGACATGTTGTTGG+CGG + chr5.1:5138495-5138514 MS.gene40603:CDS 45.0%
CTAGATGGACATATCGTTGG+AGG + chr5.1:5137128-5137147 MS.gene40603:CDS 45.0%
GACTTCAACGTCAATGCCAA+CGG + chr5.1:5137515-5137534 MS.gene40603:CDS 45.0%
GGTCTAGATGGACATATCGT+TGG + chr5.1:5137125-5137144 MS.gene40603:CDS 45.0%
TCATGCGAGTTTCATGGTTG+TGG + chr5.1:5137623-5137642 MS.gene40603:intron 45.0%
TGAGATGTCGAATCAGCTGT+GGG - chr5.1:5135991-5136010 None:intergenic 45.0%
TGAGCAGAAGCTAAAAACGC+AGG + chr5.1:5137482-5137501 MS.gene40603:CDS 45.0%
TTCATGCCAAAGGAAAAGCG+CGG + chr5.1:5138429-5138448 MS.gene40603:CDS 45.0%
TTTGGACCAGCAGTAGTCAT+GGG - chr5.1:5138892-5138911 None:intergenic 45.0%
! GTTTCTTTAGCGGGTCTAGA+TGG + chr5.1:5137113-5137132 MS.gene40603:CDS 45.0%
!! ACAGCTGATTCGACATCTCA+GGG + chr5.1:5135991-5136010 MS.gene40603:CDS 45.0%
!! ATGGAGCTAGATGTTGGCTT+GGG - chr5.1:5136114-5136133 None:intergenic 45.0%
!! GGTAGTGGTAAGCAGTTTTC+TGG + chr5.1:5137446-5137465 MS.gene40603:intron 45.0%
ACAGGACTTGCAGCTACCAA+TGG - chr5.1:5137167-5137186 None:intergenic 50.0%
CCGTCTGGTCCATACTTTCT+GGG - chr5.1:5136069-5136088 None:intergenic 50.0%
CGATTGAGGCTGACTAATGG+TGG - chr5.1:5136865-5136884 None:intergenic 50.0%
CGTCTGGTCCATACTTTCTG+GGG - chr5.1:5136068-5136087 None:intergenic 50.0%
CTGAGATGTCGAATCAGCTG+TGG - chr5.1:5135992-5136011 None:intergenic 50.0%
GAATTTGGCAGCTTTGCGGA+TGG - chr5.1:5136133-5136152 None:intergenic 50.0%
GCCAAGATTAAAGACCCGTC+TGG - chr5.1:5136084-5136103 None:intergenic 50.0%
TAAGTACCTGGTCCTGGCTA+GGG + chr5.1:5138559-5138578 MS.gene40603:intron 50.0%
TCTTCAATAGGGCCCTCATG+AGG - chr5.1:5136533-5136552 None:intergenic 50.0%
TGCGAGTTTCATGGTTGTGG+AGG + chr5.1:5137626-5137645 MS.gene40603:intron 50.0%
TTGCGGATGGAGCTAGATGT+TGG - chr5.1:5136120-5136139 None:intergenic 50.0%
! CCAGAAAGTATGGACCAGAC+GGG + chr5.1:5136067-5136086 MS.gene40603:CDS 50.0%
! CCCAGAAAGTATGGACCAGA+CGG + chr5.1:5136066-5136085 MS.gene40603:CDS 50.0%
! CTAGAGCATAAGGTGCCAGA+AGG + chr5.1:5136575-5136594 MS.gene40603:CDS 50.0%
! GATGGAGCTAGATGTTGGCT+TGG - chr5.1:5136115-5136134 None:intergenic 50.0%
!! ATGGAGGGTGCTAGCAATAG+TGG + chr5.1:5135898-5135917 MS.gene40603:CDS 50.0%
!! ATTCCAGTTCTTGGTGCTGC+AGG - chr5.1:5135949-5135968 None:intergenic 50.0%
!! CACAGCTGATTCGACATCTC+AGG + chr5.1:5135990-5136009 MS.gene40603:CDS 50.0%
!! TTCCAGTTCTTGGTGCTGCA+GGG - chr5.1:5135948-5135967 None:intergenic 50.0%
AACCCTGCAGCACCAAGAAC+TGG + chr5.1:5135943-5135962 MS.gene40603:CDS 55.0%
ACAGGACTTGCAGCCACCAA+TGG - chr5.1:5138534-5138553 None:intergenic 55.0%
AGGCATTCCACCGCTGCAAA+TGG + chr5.1:5136014-5136033 MS.gene40603:CDS 55.0%
AGGTTGCGTAGCTGGTCCAT+TGG + chr5.1:5137148-5137167 MS.gene40603:CDS 55.0%
ATCGTTGGAGGTTGCGTAGC+TGG + chr5.1:5137140-5137159 MS.gene40603:CDS 55.0%
CATATTCCCTAGCCAGGACC+AGG - chr5.1:5138568-5138587 None:intergenic 55.0%
CATTCCACCGCTGCAAATGG+TGG + chr5.1:5136017-5136036 MS.gene40603:CDS 55.0%
CCAAACGAAAGTCGCAGTGG+TGG + chr5.1:5137083-5137102 MS.gene40603:CDS 55.0%
CCACCACTGCGACTTTCGTT+TGG - chr5.1:5137086-5137105 None:intergenic 55.0%
CCCGTCTGGTCCATACTTTC+TGG - chr5.1:5136070-5136089 None:intergenic 55.0%
CGAATCAGCTGTGGGTCCAT+CGG - chr5.1:5135983-5136002 None:intergenic 55.0%
GCGCCAGCAAAGAGGAAAAG+GGG + chr5.1:5136039-5136058 MS.gene40603:CDS 55.0%
GGAAGGCGGAGTATATCTGG+TGG + chr5.1:5138450-5138469 MS.gene40603:CDS 55.0%
GGCGCCAGCAAAGAGGAAAA+GGG + chr5.1:5136038-5136057 MS.gene40603:CDS 55.0%
GTAAGTACCTGGTCCTGGCT+AGG + chr5.1:5138558-5138577 MS.gene40603:intron 55.0%
GTGCCAAACGAAAGTCGCAG+TGG + chr5.1:5137080-5137099 MS.gene40603:CDS 55.0%
TCGTGTAGCTGGTCCATTGG+TGG + chr5.1:5138518-5138537 MS.gene40603:CDS 55.0%
TGGCGCCAGCAAAGAGGAAA+AGG + chr5.1:5136037-5136056 MS.gene40603:CDS 55.0%
TGGGTCCATCGGCAAAAGCT+GGG - chr5.1:5135972-5135991 None:intergenic 55.0%
! AAATGGTGGCGCCAGCAAAG+AGG + chr5.1:5136031-5136050 MS.gene40603:CDS 55.0%
! CAAAGGAAAAGCGCGGAAGG+CGG + chr5.1:5138436-5138455 MS.gene40603:CDS 55.0%
! CGACCCCTTTTCCTCTTTGC+TGG - chr5.1:5136045-5136064 None:intergenic 55.0%
! GGAATCCCAGCTTTTGCCGA+TGG + chr5.1:5135964-5135983 MS.gene40603:CDS 55.0%
! GGTGCTGCAGGGTTATCTGT+TGG - chr5.1:5135937-5135956 None:intergenic 55.0%
! TGCCAAAGGAAAAGCGCGGA+AGG + chr5.1:5138433-5138452 MS.gene40603:CDS 55.0%
!! GAGCTAGATGTTGGCTTGGG+AGG - chr5.1:5136111-5136130 None:intergenic 55.0%
!! GCTGATTCGACATCTCAGGG+AGG + chr5.1:5135994-5136013 MS.gene40603:CDS 55.0%
AGGGGTCGCCCCAGAAAGTA+TGG + chr5.1:5136057-5136076 MS.gene40603:CDS 60.0%
CGCGGAAGGCGGAGTATATC+TGG + chr5.1:5138447-5138466 MS.gene40603:CDS 60.0%
CGGTCGTGTAGCTGGTCCAT+TGG + chr5.1:5138515-5138534 MS.gene40603:CDS 60.0%
GATGCTCCCGTGTCTACTGC+TGG + chr5.1:5137540-5137559 MS.gene40603:CDS 60.0%
GCAGTAGTCATGGGAGCAGC+TGG - chr5.1:5138883-5138902 None:intergenic 60.0%
GCTGCTCCCATGACTACTGC+TGG + chr5.1:5138883-5138902 MS.gene40603:CDS 60.0%
GTGGGTCCATCGGCAAAAGC+TGG - chr5.1:5135973-5135992 None:intergenic 60.0%
GTTGTTGGCGGTCGTGTAGC+TGG + chr5.1:5138507-5138526 MS.gene40603:CDS 60.0%
! CGCCTTCCGCGCTTTTCCTT+TGG - chr5.1:5138438-5138457 None:intergenic 60.0%
GCTGGCGCCACCATTTGCAG+CGG - chr5.1:5136027-5136046 None:intergenic 65.0%
!! GACACGGGAGCATCTGCCGT+TGG - chr5.1:5137534-5137553 None:intergenic 65.0%
GGCGCCACCATTTGCAGCGG+TGG - chr5.1:5136024-5136043 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr5.1 gene 5135898 5138918 5135898 ID=MS.gene40603
chr5.1 mRNA 5135898 5138918 5135898 ID=MS.gene40603.t1;Parent=MS.gene40603
chr5.1 exon 5135898 5136168 5135898 ID=MS.gene40603.t1.exon1;Parent=MS.gene40603.t1
chr5.1 CDS 5135898 5136168 5135898 ID=cds.MS.gene40603.t1;Parent=MS.gene40603.t1
chr5.1 exon 5136507 5136640 5136507 ID=MS.gene40603.t1.exon2;Parent=MS.gene40603.t1
chr5.1 CDS 5136507 5136640 5136507 ID=cds.MS.gene40603.t1;Parent=MS.gene40603.t1
chr5.1 exon 5136777 5136908 5136777 ID=MS.gene40603.t1.exon3;Parent=MS.gene40603.t1
chr5.1 CDS 5136777 5136908 5136777 ID=cds.MS.gene40603.t1;Parent=MS.gene40603.t1
chr5.1 exon 5137044 5137190 5137044 ID=MS.gene40603.t1.exon4;Parent=MS.gene40603.t1
chr5.1 CDS 5137044 5137190 5137044 ID=cds.MS.gene40603.t1;Parent=MS.gene40603.t1
chr5.1 exon 5137447 5137561 5137447 ID=MS.gene40603.t1.exon5;Parent=MS.gene40603.t1
chr5.1 CDS 5137447 5137561 5137447 ID=cds.MS.gene40603.t1;Parent=MS.gene40603.t1
chr5.1 exon 5137834 5137901 5137834 ID=MS.gene40603.t1.exon6;Parent=MS.gene40603.t1
chr5.1 CDS 5137834 5137901 5137834 ID=cds.MS.gene40603.t1;Parent=MS.gene40603.t1
chr5.1 exon 5138114 5138245 5138114 ID=MS.gene40603.t1.exon7;Parent=MS.gene40603.t1
chr5.1 CDS 5138114 5138245 5138114 ID=cds.MS.gene40603.t1;Parent=MS.gene40603.t1
chr5.1 exon 5138396 5138557 5138396 ID=MS.gene40603.t1.exon8;Parent=MS.gene40603.t1
chr5.1 CDS 5138396 5138557 5138396 ID=cds.MS.gene40603.t1;Parent=MS.gene40603.t1
chr5.1 exon 5138802 5138918 5138802 ID=MS.gene40603.t1.exon9;Parent=MS.gene40603.t1
chr5.1 CDS 5138802 5138918 5138802 ID=cds.MS.gene40603.t1;Parent=MS.gene40603.t1
Gene Sequence

>MS.gene40603

ATGGAGGGTGCTAGCAATAGTGGTGCTTCAGATACTCCAACAGATAACCCTGCAGCACCAAGAACTGGAATCCCAGCTTTTGCCGATGGACCCACAGCTGATTCGACATCTCAGGGAGGCATTCCACCGCTGCAAATGGTGGCGCCAGCAAAGAGGAAAAGGGGTCGCCCCAGAAAGTATGGACCAGACGGGTCTTTAATCTTGGCGATACCTCCCAAGCCAACATCTAGCTCCATCCGCAAAGCTGCCAAATTCGAGTTGGAGAATCCAGGTAGCTAAGAAATTCTCTTTCTTTGTGATCTTGTGCATTTCTTGATTGAATGCTTGTGAATGAGTATCAAGAAAGAATAGTGCACTTTGTTGTTCGGAATGTTCAATGTTGTGTTATTAATATATATCAAGTGTATTGGTGGTACTTGCATCAAATATAAGATGTAATTGTAAATGTTGGTTCAGCTTTCATACATGTTGATTTTATTCTTTGAATAGAAACTGTGTCTGTTCATAATTTCCTGATTTCGGCATTGAATATCTTTGTGGTTAATTAGTATGATTATTTAGTGAAAGTTCTTTCTTTCTTTTTGTCTGGCCAATTTCTTGCAAATTCAGTTGGTGCTATAGTAAATTTAGATCCTCATGAGGGCCCTATTGAAGATCAAACTCAACACCCTCAAGAACTAGAGCATAAGGTGCCAGAAGGCACTACTTTTACATCCCGCATTATCACAGTTAATTCTGGAGAGGTAATATCTTCGATATTCATAATTGGTAGATGCACAAATTTAACTAAAATTTCTTGCAGTAATTATATTTATTTTCTATATAACTGTAATGACTGATTAGAATAAATGGCTACTACTTGTCTTTTGGTTGTCGCAGAATATTACTATGAAAGTAATGTCATTTTGTCAGCAAGGACCAGAAGCTATATGCATTCTATCTGCAAATGGAGTCATATCAAGCGCCACCATTAGTCAGCCTCAATCGTCTCAAAAATTATCCACATATGAGGTGAAGCATTTCTATAATCTGATAGTAGTATTATTGGTATCAGTTTTAATATGGAGTTTAGGTTATATAGCTTGTATTCAAATTTTGTTTGCCTGTTTTGATGTGTTTCTTTAAATAATGTCAATTGAATTTCAGGGTAAATATGAAATTATATCTTTATCTGGCTCATTCGTGCCAAACGAAAGTCGCAGTGGTGGTATGAGTGTTTCTTTAGCGGGTCTAGATGGACATATCGTTGGAGGTTGCGTAGCTGGTCCATTGGTAGCTGCAAGTCCTGTAAATGTAAGTACCTGGAAAGGGAATAATAGGGCTAATATGTTTTTCACATACTAAAACACCAATTAGCAGGATGCTTAAATCTTAATTATGTAGATATCTTTTGAAAAGTAAAGTTGATGATCTAGTAACATTATTAGTGTCCTGCTAACGAAATTGCTACTATCCAGTGGTAGTAAAACGCAAACTTTGTGGTAACTAATTCTCAGTGACAAGTCTATGCAATTCTCATTACTCTTTCCAATGGATTGATATGTATCAGGTAGTGGTAAGCAGTTTTCTGGCAAATGAACAGTCTGAGCAGAAGCTAAAAACGCAGGAAAATGAGGCGACTTCAACGTCAATGCCAACGGCAGATGCTCCCGTGTCTACTGCTGGTACAATACCTAACTCTCATCTTCATCTTCCTTCCACGGAGATGATTCTCTTTCTTTGTGATCATGCGAGTTTCATGGTTGTGGAGGAGTATCAAGAATAGTGTATTTTGTTGTTTGGAATGTTTAATATTATATTATTAATATATATCAAGTTATTGGTGGTACTTGCATCAATTTAAGATATAAGTGTAAATGTTGGTTCAGCTTTCTTACATGTTGATTGTATTCTTAAATGTTGATTATTTAGTGAAAGTGCTTTCTTTTTTTCTCAGGCCAATTGCTTGCAAGCTCAGTTAGTGCTACTTTTACACCTCATATTTTCATTGTGAATGCTGGAGAGGTAATATCATTTTTTGGATTTTTATGATGCAAAGAAATTCTATTTCCCATAATATTCATAATTAGATAAGTGAATATATATCTTGATATTTTTAACTGGTCAATGCACAACTTTAACTACAATTTCTTGCAATAATTGTATTTGTTTCCGTTATCACTGGAATGATGACTAATCAGAATAAATGGCTACTACTTGTCTTTTGATTGTCTCAGGATGTTTCTATGAAGATAATGTCATTTTGTCAGCAAGGACCAGAAGCTATATGCATTCTATCTGTGAATGGAGTCATATCAAAAGCAGTCATTCGTCGGCCTCAATCGTCTCGAACATTATTCACGTATGAGGTGAAGCATTTATGTAATAATCTGATAGTTGTAGTAGTATCAACTTTTCCTAATTCAATGTACTAATGTGGAGTTTAGGACCTTAGGAGTAGTTTGTTATTCATATGCTTTTTGTATGTGATTCTTTCAATAATGCAAATTGAATTTCAGGGTAGATATGAAATTCGAACTTTATCTGGTTTGTTCATGCCAAAGGAAAAGCGCGGAAGGCGGAGTATATCTGGTGGCATGAGTGTTTCTTTAGTGGATCTACATGGACATGTTGTTGGCGGTCGTGTAGCTGGTCCATTGGTGGCTGCAAGTCCTGTAAATGTAAGTACCTGGTCCTGGCTAGGGAATATGTCTTTTTCGCAAACTAAAGTCTAGCAGGATGCTTATGTAGTTATTTATCTTGGATAAGTAAAGTTGATGATTTAGTAACATTATCTGTTTCCTGCTAAAGAAATGGCAACTATATAACAAATTTATGCAATTCTGATAATTTAGTGGTAATTAGTGCTAAGTGACAAGTTAATGCAATTCTCATCACTGTTTCCAATGGATTGATATGTATCAGGTAGTGGTAGGCAGTTTTCTGCCAAGTGAACAACATGAGCAGAAGCTAAAAACACAGAATAATGAGGTAATTTCAACTCCAGCTGCTCCCATGACTACTGCTGGTCCAAATACCTAA
Protein sequence

>MS.gene40603.t1

MEGASNSGASDTPTDNPAAPRTGIPAFADGPTADSTSQGGIPPLQMVAPAKRKRGRPRKYGPDGSLILAIPPKPTSSSIRKAAKFELENPVGAIVNLDPHEGPIEDQTQHPQELEHKVPEGTTFTSRIITVNSGENITMKVMSFCQQGPEAICILSANGVISSATISQPQSSQKLSTYEGKYEIISLSGSFVPNESRSGGMSVSLAGLDGHIVGGCVAGPLVAASPVNVVVSSFLANEQSEQKLKTQENEATSTSMPTADAPVSTAGQLLASSVSATFTPHIFIVNAGEDVSMKIMSFCQQGPEAICILSVNGVISKAVIRRPQSSRTLFTYEGRYEIRTLSGLFMPKEKRGRRSISGGMSVSLVDLHGHVVGGRVAGPLVAASPVNVVVGSFLPSEQHEQKLKTQNNEVISTPAAPMTTAGPNT