Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene41039.t1 | GAU44816.1 | 62.4 | 93 | 26 | 2 | 7 | 90 | 1 | 93 | 6.50E-20 | 106.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene41039.t1 | A0A2Z6NMD3 | 62.4 | 93 | 26 | 2 | 7 | 90 | 1 | 93 | 4.7e-20 | 106.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene055056 | MS.gene41039 | 0.8008 | 1.22E-48 | -1.69E-46 |
| MS.gene055058 | MS.gene41039 | 0.802845 | 4.62E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 32 sgRNAs with CRISPR-Local
Find 44 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CATCTATGTTGGTGGTAAAA+TGG | 0.234195 | 1.3:+41793820 | MS.gene41039:CDS |
| TATCACGGAAAATGAGTTCA+TGG | 0.368247 | 1.3:-41793168 | None:intergenic |
| AAGGCGAAGGTGGTGGAGAA+AGG | 0.417691 | 1.3:+41793203 | MS.gene41039:CDS |
| CGTGATAAATTCATCTATGA+AGG | 0.418830 | 1.3:+41793184 | MS.gene41039:CDS |
| ATATCATGGTGGGTTGTTCA+TGG | 0.434787 | 1.3:+41793784 | MS.gene41039:CDS |
| CAAAACTGATTGAAAGTCTA+GGG | 0.463478 | 1.3:+41793894 | MS.gene41039:CDS |
| TCAAAACTGATTGAAAGTCT+AGG | 0.466346 | 1.3:+41793893 | MS.gene41039:CDS |
| GAGATGCATCTCTGTATTCA+AGG | 0.488933 | 1.3:+41793237 | MS.gene41039:CDS |
| AGGTGGTGGAGAAAGGAGAA+GGG | 0.490083 | 1.3:+41793210 | MS.gene41039:CDS |
| AAGGTGGTGGAGAAAGGAGA+AGG | 0.492351 | 1.3:+41793209 | MS.gene41039:CDS |
| ATCTATGAAGGCGAAGGTGG+TGG | 0.496255 | 1.3:+41793196 | MS.gene41039:CDS |
| GCTTGTGATCCTCGAGGTTG+AGG | 0.515924 | 1.3:-41793936 | None:intergenic |
| CCACCAACATAGATGTTGCT+AGG | 0.517120 | 1.3:-41793812 | None:intergenic |
| TGTCGATGTCGTGTCCTACA+TGG | 0.518273 | 1.3:+41793865 | MS.gene41039:CDS |
| TGGAAGAAATGAATTGTGGT+TGG | 0.540180 | 1.3:+41793840 | MS.gene41039:CDS |
| GTCATATTACGTATATATCA+TGG | 0.541353 | 1.3:+41793770 | MS.gene41039:CDS |
| CCTAGCAACATCTATGTTGG+TGG | 0.553229 | 1.3:+41793812 | MS.gene41039:CDS |
| TTCATCTATGAAGGCGAAGG+TGG | 0.561267 | 1.3:+41793193 | MS.gene41039:CDS |
| GTATAGGCCATCGCAAAGGC+AGG | 0.562742 | 1.3:+41793748 | MS.gene41039:intron |
| GTGGTGGAGAAAGGAGAAGG+GGG | 0.563580 | 1.3:+41793212 | MS.gene41039:CDS |
| GGAAGAAATGAATTGTGGTT+GGG | 0.566329 | 1.3:+41793841 | MS.gene41039:CDS |
| TCACCTAGCAACATCTATGT+TGG | 0.577638 | 1.3:+41793809 | MS.gene41039:CDS |
| CTTCATAGATGAATTTATCA+CGG | 0.579781 | 1.3:-41793183 | None:intergenic |
| AAAATGGAAGAAATGAATTG+TGG | 0.611686 | 1.3:+41793836 | MS.gene41039:CDS |
| AATATGACCTGCCTTTGCGA+TGG | 0.616950 | 1.3:-41793755 | None:intergenic |
| AAATTCATCTATGAAGGCGA+AGG | 0.625676 | 1.3:+41793190 | MS.gene41039:CDS |
| GGTGGTGGAGAAAGGAGAAG+GGG | 0.655309 | 1.3:+41793211 | MS.gene41039:CDS |
| TATTACGTATATATCATGGT+GGG | 0.655518 | 1.3:+41793774 | MS.gene41039:CDS |
| ATTTGTATAGGCCATCGCAA+AGG | 0.683372 | 1.3:+41793744 | MS.gene41039:intron |
| TCAGAAGCACCTCAACCTCG+AGG | 0.701100 | 1.3:+41793927 | MS.gene41039:CDS |
| ATATTACGTATATATCATGG+TGG | 0.713718 | 1.3:+41793773 | MS.gene41039:CDS |
| ACTTATGCTTGTGATCCTCG+AGG | 0.715140 | 1.3:-41793942 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TGTATGTTCTTTATTTGTAT+AGG | + | chr1.3:41793732-41793751 | MS.gene41039:intron | 20.0% |
| !!! | TTCACAATTTTTATTCGTTA+TGG | - | chr1.3:41793325-41793344 | None:intergenic | 20.0% |
| ! | AAAATGGAAGAAATGAATTG+TGG | + | chr1.3:41793836-41793855 | MS.gene41039:CDS | 25.0% |
| ! | AATCAAAATAGCTACTGTAA+AGG | - | chr1.3:41793596-41793615 | None:intergenic | 25.0% |
| ! | ATATTACGTATATATCATGG+TGG | + | chr1.3:41793773-41793792 | MS.gene41039:CDS | 25.0% |
| ! | CTTCATAGATGAATTTATCA+CGG | - | chr1.3:41793186-41793205 | None:intergenic | 25.0% |
| ! | GTCATATTACGTATATATCA+TGG | + | chr1.3:41793770-41793789 | MS.gene41039:CDS | 25.0% |
| ! | TATTACGTATATATCATGGT+GGG | + | chr1.3:41793774-41793793 | MS.gene41039:CDS | 25.0% |
| !!! | GATTTTGTATCTCTAGTTTA+GGG | + | chr1.3:41793401-41793420 | MS.gene41039:intron | 25.0% |
| !!! | TACAGTAGCTATTTTGATTT+TGG | + | chr1.3:41793597-41793616 | MS.gene41039:intron | 25.0% |
| !!! | TGATTTTGTATCTCTAGTTT+AGG | + | chr1.3:41793400-41793419 | MS.gene41039:intron | 25.0% |
| CAAAACTGATTGAAAGTCTA+GGG | + | chr1.3:41793894-41793913 | MS.gene41039:CDS | 30.0% | |
| CGTGATAAATTCATCTATGA+AGG | + | chr1.3:41793184-41793203 | MS.gene41039:CDS | 30.0% | |
| TCAAAACTGATTGAAAGTCT+AGG | + | chr1.3:41793893-41793912 | MS.gene41039:CDS | 30.0% | |
| ! | TTTTCTCTTTTGACCCAATA+TGG | + | chr1.3:41793270-41793289 | MS.gene41039:intron | 30.0% |
| !!! | CAGTTTTGAAATATCCATGT+AGG | - | chr1.3:41793882-41793901 | None:intergenic | 30.0% |
| !!! | TGTTCTTGCTACTGATTTTA+TGG | + | chr1.3:41793644-41793663 | MS.gene41039:intron | 30.0% |
| AAATTCATCTATGAAGGCGA+AGG | + | chr1.3:41793190-41793209 | MS.gene41039:CDS | 35.0% | |
| ACACCTTAACTACACCATAT+TGG | - | chr1.3:41793287-41793306 | None:intergenic | 35.0% | |
| CACCTTAACTACACCATATT+GGG | - | chr1.3:41793286-41793305 | None:intergenic | 35.0% | |
| GGAAGAAATGAATTGTGGTT+GGG | + | chr1.3:41793841-41793860 | MS.gene41039:CDS | 35.0% | |
| TATCACAATAGCTACTGTGA+AGG | - | chr1.3:41793519-41793538 | None:intergenic | 35.0% | |
| TGGAAGAAATGAATTGTGGT+TGG | + | chr1.3:41793840-41793859 | MS.gene41039:CDS | 35.0% | |
| !! | CATCTATGTTGGTGGTAAAA+TGG | + | chr1.3:41793820-41793839 | MS.gene41039:CDS | 35.0% |
| ATTTGTATAGGCCATCGCAA+AGG | + | chr1.3:41793744-41793763 | MS.gene41039:intron | 40.0% | |
| GACCCAATATGGTGTAGTTA+AGG | + | chr1.3:41793281-41793300 | MS.gene41039:intron | 40.0% | |
| GAGATGCATCTCTGTATTCA+AGG | + | chr1.3:41793237-41793256 | MS.gene41039:CDS | 40.0% | |
| TCACCTAGCAACATCTATGT+TGG | + | chr1.3:41793809-41793828 | MS.gene41039:CDS | 40.0% | |
| !! | ATATCATGGTGGGTTGTTCA+TGG | + | chr1.3:41793784-41793803 | MS.gene41039:CDS | 40.0% |
| AATATGACCTGCCTTTGCGA+TGG | - | chr1.3:41793758-41793777 | None:intergenic | 45.0% | |
| ACTTATGCTTGTGATCCTCG+AGG | - | chr1.3:41793945-41793964 | None:intergenic | 45.0% | |
| CCACCAACATAGATGTTGCT+AGG | - | chr1.3:41793815-41793834 | None:intergenic | 45.0% | |
| CCTAGCAACATCTATGTTGG+TGG | + | chr1.3:41793812-41793831 | MS.gene41039:CDS | 45.0% | |
| TTCATCTATGAAGGCGAAGG+TGG | + | chr1.3:41793193-41793212 | MS.gene41039:CDS | 45.0% | |
| ATCTATGAAGGCGAAGGTGG+TGG | + | chr1.3:41793196-41793215 | MS.gene41039:CDS | 50.0% | |
| TGTCGATGTCGTGTCCTACA+TGG | + | chr1.3:41793865-41793884 | MS.gene41039:CDS | 50.0% | |
| !! | AAGGTGGTGGAGAAAGGAGA+AGG | + | chr1.3:41793209-41793228 | MS.gene41039:CDS | 50.0% |
| !! | AGGTGGTGGAGAAAGGAGAA+GGG | + | chr1.3:41793210-41793229 | MS.gene41039:CDS | 50.0% |
| GCTTGTGATCCTCGAGGTTG+AGG | - | chr1.3:41793939-41793958 | None:intergenic | 55.0% | |
| GTATAGGCCATCGCAAAGGC+AGG | + | chr1.3:41793748-41793767 | MS.gene41039:intron | 55.0% | |
| ! | GTGGTGGAGAAAGGAGAAGG+GGG | + | chr1.3:41793212-41793231 | MS.gene41039:CDS | 55.0% |
| !! | AAGGCGAAGGTGGTGGAGAA+AGG | + | chr1.3:41793203-41793222 | MS.gene41039:CDS | 55.0% |
| !! | GGTGGTGGAGAAAGGAGAAG+GGG | + | chr1.3:41793211-41793230 | MS.gene41039:CDS | 55.0% |
| !! | TCAGAAGCACCTCAACCTCG+AGG | + | chr1.3:41793927-41793946 | MS.gene41039:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.3 | gene | 41793170 | 41793970 | 41793170 | ID=MS.gene41039 |
| chr1.3 | mRNA | 41793170 | 41793970 | 41793170 | ID=MS.gene41039.t1;Parent=MS.gene41039 |
| chr1.3 | exon | 41793170 | 41793258 | 41793170 | ID=MS.gene41039.t1.exon1;Parent=MS.gene41039.t1 |
| chr1.3 | CDS | 41793170 | 41793258 | 41793170 | ID=cds.MS.gene41039.t1;Parent=MS.gene41039.t1 |
| chr1.3 | exon | 41793754 | 41793970 | 41793754 | ID=MS.gene41039.t1.exon2;Parent=MS.gene41039.t1 |
| chr1.3 | CDS | 41793754 | 41793970 | 41793754 | ID=cds.MS.gene41039.t1;Parent=MS.gene41039.t1 |
| Gene Sequence |
| Protein sequence |
