Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene41210.t1 | XP_013467848.1 | 96.9 | 131 | 4 | 0 | 1 | 131 | 116 | 246 | 6.80E-62 | 246.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene41210.t1 | Q9SF15 | 32.3 | 130 | 80 | 3 | 2 | 127 | 123 | 248 | 3.5e-14 | 79.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene41210.t1 | A0A072VUZ2 | 96.9 | 131 | 4 | 0 | 1 | 131 | 116 | 246 | 4.9e-62 | 246.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene054892 | MS.gene41210 | 0.801154 | 1.03E-48 | -1.69E-46 |
| MS.gene054893 | MS.gene41210 | 0.800298 | 1.54E-48 | -1.69E-46 |
| MS.gene060412 | MS.gene41210 | 0.808256 | 3.39E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene41210.t1 | MTR_1g056910 | 96.947 | 131 | 4 | 0 | 1 | 131 | 116 | 246 | 1.66e-85 | 255 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene41210.t1 | AT3G11840 | 32.308 | 130 | 80 | 3 | 2 | 127 | 123 | 248 | 2.77e-18 | 79.7 |
Find 11 sgRNAs with CRISPR-Local
Find 17 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CACAATTCTTTCGAAGAATT+CGG | 0.199521 | 1.2:+45005731 | None:intergenic |
| ATTTGAAAATAATCAAATAC+TGG | 0.314035 | 1.2:-45005877 | MS.gene41210:CDS |
| GCTCAAGAGTTTAATGCAGT+TGG | 0.418910 | 1.2:-45006081 | None:intergenic |
| ATTGTGAAAGTTCTAAGAAA+TGG | 0.433892 | 1.2:-45005714 | MS.gene41210:CDS |
| ACAATTCTTTCGAAGAATTC+GGG | 0.437523 | 1.2:+45005732 | None:intergenic |
| ATGAACATAATCATCTCTTT+CGG | 0.450958 | 1.2:+45006002 | None:intergenic |
| GAAGGTATTGAAGAAGCTCT+TGG | 0.482882 | 1.2:-45005948 | MS.gene41210:CDS |
| AGATTCTTCACTTCTTCTGT+AGG | 0.513088 | 1.2:+45005903 | None:intergenic |
| AAATACTGGATTCTTTAACA+TGG | 0.565062 | 1.2:-45005863 | MS.gene41210:CDS |
| GGTTACTGTGAAATCGCATG+CGG | 0.706124 | 1.2:-45005814 | MS.gene41210:CDS |
| AATGGTGTAATAACACAACA+AGG | 0.719258 | 1.2:-45005696 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATTTGAAAATAATCAAATAC+TGG | - | chr1.2:45005888-45005907 | MS.gene41210:CDS | 15.0% |
| !! | ATAGTTGATTGTTACAAAAA+AGG | - | chr1.2:45005784-45005803 | MS.gene41210:CDS | 20.0% |
| !!! | TGGTTTTTTACAATTTGTTA+AGG | - | chr1.2:45005837-45005856 | MS.gene41210:CDS | 20.0% |
| ! | AAAAAAGGCGAAATTTATGA+AGG | - | chr1.2:45005799-45005818 | MS.gene41210:CDS | 25.0% |
| ! | AAATACTGGATTCTTTAACA+TGG | - | chr1.2:45005902-45005921 | MS.gene41210:CDS | 25.0% |
| ! | ATGAACATAATCATCTCTTT+CGG | + | chr1.2:45005766-45005785 | None:intergenic | 25.0% |
| !! | AGAAAGATTTTGTTGGAAAA+TGG | - | chr1.2:45005736-45005755 | MS.gene41210:CDS | 25.0% |
| !!! | ATTGTGAAAGTTCTAAGAAA+TGG | - | chr1.2:45006051-45006070 | MS.gene41210:CDS | 25.0% |
| ACAATTCTTTCGAAGAATTC+GGG | + | chr1.2:45006036-45006055 | None:intergenic | 30.0% | |
| CACAATTCTTTCGAAGAATT+CGG | + | chr1.2:45006037-45006056 | None:intergenic | 30.0% | |
| ! | GAGCAATAGAAAGATTTTGT+TGG | - | chr1.2:45005729-45005748 | MS.gene41210:CDS | 30.0% |
| AGATTCTTCACTTCTTCTGT+AGG | + | chr1.2:45005865-45005884 | None:intergenic | 35.0% | |
| AGTTTCCGAGATGAAAAACT+CGG | - | chr1.2:45005930-45005949 | MS.gene41210:CDS | 35.0% | |
| ! | AGTAACCGAGTTTTTCATCT+CGG | + | chr1.2:45005938-45005957 | None:intergenic | 35.0% |
| !! | GAAGGTATTGAAGAAGCTCT+TGG | - | chr1.2:45005817-45005836 | MS.gene41210:CDS | 40.0% |
| !!! | GCGATTCAATCGTTTTAGAG+AGG | - | chr1.2:45006004-45006023 | MS.gene41210:CDS | 40.0% |
| GGTTACTGTGAAATCGCATG+CGG | - | chr1.2:45005951-45005970 | MS.gene41210:CDS | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.2 | gene | 45005697 | 45006090 | 45005697 | ID=MS.gene41210 |
| chr1.2 | mRNA | 45005697 | 45006090 | 45005697 | ID=MS.gene41210.t1;Parent=MS.gene41210 |
| chr1.2 | exon | 45005697 | 45006090 | 45005697 | ID=MS.gene41210.t1.exon1;Parent=MS.gene41210.t1 |
| chr1.2 | CDS | 45005697 | 45006090 | 45005697 | ID=cds.MS.gene41210.t1;Parent=MS.gene41210.t1 |
| Gene Sequence |
| Protein sequence |
