Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene054801 | MS.gene41450 | 0.815496 | 8.97E-52 | -1.69E-46 |
| MS.gene058515 | MS.gene41450 | 0.86556 | 5.41E-65 | -1.69E-46 |
| MS.gene059276 | MS.gene41450 | 0.80145 | 8.95E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene41450.t1 | MTR_3g063010 | 79.592 | 49 | 10 | 0 | 718 | 766 | 1481 | 1529 | 4.10e-15 | 80.5 |
| MS.gene41450.t1 | MTR_3g062390 | 77.083 | 48 | 11 | 0 | 716 | 763 | 132 | 179 | 4.24e-15 | 74.7 |
| MS.gene41450.t1 | MTR_3g062420 | 80.392 | 51 | 10 | 0 | 716 | 766 | 1500 | 1550 | 5.49e-15 | 80.1 |
| MS.gene41450.t1 | MTR_3g062980 | 78.431 | 51 | 11 | 0 | 716 | 766 | 391 | 441 | 1.01e-14 | 78.6 |
| MS.gene41450.t1 | MTR_3g062340 | 71.154 | 52 | 15 | 0 | 715 | 766 | 1406 | 1457 | 1.16e-14 | 79.0 |
| MS.gene41450.t1 | MTR_3g062340 | 29.836 | 305 | 134 | 13 | 499 | 766 | 1388 | 1649 | 3.30e-11 | 67.8 |
| MS.gene41450.t1 | MTR_3g062230 | 74.510 | 51 | 13 | 0 | 716 | 766 | 447 | 497 | 1.22e-14 | 78.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 87 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACAAGTTTATCCTCTGTTTC+CGG | 0.243984 | 3.1:-52328541 | None:intergenic |
| CATATTACTTCTGAATTTGT+AGG | 0.248613 | 3.1:-52333255 | None:intergenic |
| ACAAGTTTATGCTCTGTTTC+CGG | 0.250025 | 3.1:-52329579 | None:intergenic |
| AAGAGTTTATGCTCTGTTTC+CGG | 0.253676 | 3.1:-52331308 | None:intergenic |
| CAAATTACTTCTGAATTTGT+AGG | 0.254985 | 3.1:-52329108 | None:intergenic |
| GCAATTACTTCTGTGTTTGT+AGG | 0.277861 | 3.1:-52332910 | None:intergenic |
| TGTTGAAAATGTTCCTCATT+TGG | 0.281126 | 3.1:+52328561 | MS.gene41450:CDS |
| CAAATTACTTCTGAATTTGT+AGG | 0.286863 | 3.1:-52328070 | None:intergenic |
| CAAATTACTTCTGAATTTGT+AGG | 0.286863 | 3.1:-52328416 | None:intergenic |
| CAAATTACTTCTGAATTTGT+AGG | 0.286863 | 3.1:-52329454 | None:intergenic |
| CAAATTACTTCTGAATTTGT+AGG | 0.286863 | 3.1:-52330146 | None:intergenic |
| CAAATTACTTCTGAATTTGT+AGG | 0.286863 | 3.1:-52330491 | None:intergenic |
| CAAATTACTTCTGAATTTGT+AGG | 0.286863 | 3.1:-52331183 | None:intergenic |
| CAAATTACTTCTGAATTTGT+AGG | 0.286863 | 3.1:-52331872 | None:intergenic |
| CAAATTACTTCTGAATTTGT+AGG | 0.286863 | 3.1:-52332218 | None:intergenic |
| CAAATTACTTCTGAATTTGT+AGG | 0.286863 | 3.1:-52333601 | None:intergenic |
| TTGTTACAGGAATTGATTAA+TGG | 0.288339 | 3.1:+52334598 | MS.gene41450:intron |
| CAAATTACTTCTAAATTTGT+TGG | 0.317457 | 3.1:-52333428 | None:intergenic |
| CAAAATACTTCTAAATTTGT+TGG | 0.331469 | 3.1:-52332045 | None:intergenic |
| CAAATTACTTCTGAACTTGT+AGG | 0.337011 | 3.1:-52329800 | None:intergenic |
| CAAATTACTTCTGAACTTGT+AGG | 0.337011 | 3.1:-52331528 | None:intergenic |
| GAAATTACTTCTGAATTTGT+AGG | 0.340132 | 3.1:-52332564 | None:intergenic |
| CAAATTACTTCTGAATTTGT+TGG | 0.342676 | 3.1:-52328589 | None:intergenic |
| CAAATTACTTCTGAATTTGT+TGG | 0.342676 | 3.1:-52330663 | None:intergenic |
| CAAATTACTTCTGAATTTGT+TGG | 0.342676 | 3.1:-52331356 | None:intergenic |
| CAAATTACTTCTGAATTTGT+TGG | 0.342676 | 3.1:-52332390 | None:intergenic |
| GAAATTACTTCTGAATTTGT+AGG | 0.346470 | 3.1:-52330837 | None:intergenic |
| CAAAATACTTCTGAATTTGT+TGG | 0.352011 | 3.1:-52330318 | None:intergenic |
| CAAATTACTTCTGAATTTGT+TGG | 0.361069 | 3.1:-52327899 | None:intergenic |
| CAAATTACTTCTGAATTTGT+TGG | 0.361069 | 3.1:-52329627 | None:intergenic |
| CATGCTCTGTCTCTGGTGTT+TGG | 0.364286 | 3.1:-52333200 | None:intergenic |
| ACAAGTTCATGTTCTGTCTC+TGG | 0.384599 | 3.1:-52333553 | None:intergenic |
| ACAAGTTCATGCTCTGTCTC+TGG | 0.410560 | 3.1:-52328368 | None:intergenic |
| ACAAGTTCATGCTCTGTCTC+TGG | 0.410560 | 3.1:-52329060 | None:intergenic |
| ACAAGTTCATGCTCTGTCTC+TGG | 0.410560 | 3.1:-52329406 | None:intergenic |
| ACAAGTTCATGCTCTGTCTC+TGG | 0.410560 | 3.1:-52330098 | None:intergenic |
| ACAAGTTCATGCTCTGTCTC+TGG | 0.410560 | 3.1:-52330789 | None:intergenic |
| ACAAGTTCATGCTCTGTCTC+TGG | 0.410560 | 3.1:-52331135 | None:intergenic |
| ACAAGTTCATGCTCTGTTTG+TGG | 0.413971 | 3.1:-52329752 | None:intergenic |
| ACAAGTTCATGCTCTGTTTG+TGG | 0.413971 | 3.1:-52331480 | None:intergenic |
| ACATGTTCATGCTCTGTCTC+TGG | 0.420457 | 3.1:-52332516 | None:intergenic |
| ACAAGTTGATGCTCTGTCTC+TGG | 0.423115 | 3.1:-52332862 | None:intergenic |
| ATTGCTCAACTTGTGGCCTT+CGG | 0.433062 | 3.1:-52327712 | None:intergenic |
| ACAAGTTGATGCTCTGTCTC+TGG | 0.433257 | 3.1:-52327851 | None:intergenic |
| ACAAGATCATGCTCTGTCTC+TGG | 0.436081 | 3.1:-52333207 | None:intergenic |
| CTTGTAGGTATTTCTAAATG+AGG | 0.436924 | 3.1:-52329785 | None:intergenic |
| CTTGTAGGTATTTCTAAATG+AGG | 0.436924 | 3.1:-52331513 | None:intergenic |
| TTTGTTGGTATTTCTAAATG+AGG | 0.439729 | 3.1:-52332030 | None:intergenic |
| TTTGTTGGTATTTCTAAATG+AGG | 0.439729 | 3.1:-52333413 | None:intergenic |
| GAGACAGAGCATGAACTTGT+TGG | 0.442912 | 3.1:+52331827 | MS.gene41450:CDS |
| TTTGTTGGTATTTCCAAATG+AGG | 0.446550 | 3.1:-52328574 | None:intergenic |
| TTTGTTGGTATTTCTAAATG+AGG | 0.446550 | 3.1:-52329612 | None:intergenic |
| TTTGTTGGTATTTCTAAATG+AGG | 0.446550 | 3.1:-52330303 | None:intergenic |
| TTTGTTGGTATTTCTAAATG+AGG | 0.446550 | 3.1:-52330648 | None:intergenic |
| TTTGTTGGTATTTCTAAATG+AGG | 0.446550 | 3.1:-52331341 | None:intergenic |
| TTTGTAGGTATTTCTAAATG+AGG | 0.450201 | 3.1:-52328401 | None:intergenic |
| TTTGTAGGTATTTCTAAATG+AGG | 0.450201 | 3.1:-52329093 | None:intergenic |
| TTTGTAGGTATTTCTAAATG+AGG | 0.450201 | 3.1:-52329439 | None:intergenic |
| TTTGTAGGTATTTCTAAATG+AGG | 0.450201 | 3.1:-52330131 | None:intergenic |
| TTTGTAGGTATTTCTAAATG+AGG | 0.450201 | 3.1:-52330822 | None:intergenic |
| TTTGTAGGTATTTCTAAATG+AGG | 0.450201 | 3.1:-52331168 | None:intergenic |
| TTTGTAGGTATTTCTAAATG+AGG | 0.450201 | 3.1:-52332203 | None:intergenic |
| TTTGTAGGTATTTCTAAATG+AGG | 0.450201 | 3.1:-52332549 | None:intergenic |
| ATTGATTAATGGACAATCAA+TGG | 0.452611 | 3.1:+52334609 | MS.gene41450:CDS |
| TACTGATGTCAAACCTTCTC+AGG | 0.457502 | 3.1:+52334660 | MS.gene41450:CDS |
| GAGACAGAGCATGAACTTGT+TGG | 0.463874 | 3.1:+52329063 | MS.gene41450:CDS |
| GAGACAGAGCATGAACTTGT+TGG | 0.463874 | 3.1:+52330101 | MS.gene41450:CDS |
| GAAGCAGAGCATGAACTTGT+TGG | 0.464955 | 3.1:+52328025 | MS.gene41450:CDS |
| TTTCTCCAAGTGGACGTTGT+TGG | 0.466045 | 3.1:-52334633 | None:intergenic |
| AATGAAACAAACACCGAAAA+CGG | 0.478469 | 3.1:+52332329 | MS.gene41450:CDS |
| TTTGTAGGTATTTCTAAATG+AGG | 0.481213 | 3.1:-52332895 | None:intergenic |
| TTTGTAGGTATTTCTAAATG+TGG | 0.490591 | 3.1:-52330476 | None:intergenic |
| TTTGTTGGTATCTCTAAATG+AGG | 0.493906 | 3.1:-52332375 | None:intergenic |
| ATTACATGATTTACCTGAGA+AGG | 0.496851 | 3.1:-52334673 | None:intergenic |
| ATGGACCAACAACGTCCACT+TGG | 0.568852 | 3.1:+52334628 | MS.gene41450:CDS |
| TTTGTAGGTATGTCTAAATG+AGG | 0.569286 | 3.1:-52328055 | None:intergenic |
| ACAAGTTCATGCTCTGTCTG+TGG | 0.587412 | 3.1:-52330443 | None:intergenic |
| ACAAGTTCATGCTCTGTCTG+TGG | 0.587412 | 3.1:-52332170 | None:intergenic |
| CCACAAGTTGAGCAATCCAC+AGG | 0.603608 | 3.1:+52327719 | MS.gene41450:CDS |
| CCTGTGGATTGCTCAACTTG+TGG | 0.611331 | 3.1:-52327719 | None:intergenic |
| GAAACAAACACCGGAAACAG+AGG | 0.618682 | 3.1:+52328531 | MS.gene41450:CDS |
| TCAGTATCAGTTTCTCCAAG+TGG | 0.626771 | 3.1:-52334643 | None:intergenic |
| AATGAAAATGAAACAAACAC+CGG | 0.648284 | 3.1:+52328522 | MS.gene41450:CDS |
| AATGAAAATGAAACAAACAC+CGG | 0.648284 | 3.1:+52329560 | MS.gene41450:CDS |
| AATGAAAATGAAACAAACAC+CGG | 0.648284 | 3.1:+52331289 | MS.gene41450:CDS |
| AATGAAAATGAAACAAACAT+CGG | 0.657374 | 3.1:+52328003 | MS.gene41450:CDS |
| TTAATACGAAGAAAGACCGA+AGG | 0.700958 | 3.1:+52327696 | MS.gene41450:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 52327687 | 52334681 | 52327687 | ID=MS.gene41450 |
| chr3.1 | mRNA | 52327687 | 52334681 | 52327687 | ID=MS.gene41450.t1;Parent=MS.gene41450 |
| chr3.1 | exon | 52327687 | 52327740 | 52327687 | ID=MS.gene41450.t1.exon1;Parent=MS.gene41450.t1 |
| chr3.1 | CDS | 52327687 | 52327740 | 52327687 | ID=cds.MS.gene41450.t1;Parent=MS.gene41450.t1 |
| chr3.1 | exon | 52327833 | 52327913 | 52327833 | ID=MS.gene41450.t1.exon2;Parent=MS.gene41450.t1 |
| chr3.1 | CDS | 52327833 | 52327913 | 52327833 | ID=cds.MS.gene41450.t1;Parent=MS.gene41450.t1 |
| chr3.1 | exon | 52327998 | 52328084 | 52327998 | ID=MS.gene41450.t1.exon3;Parent=MS.gene41450.t1 |
| chr3.1 | CDS | 52327998 | 52328084 | 52327998 | ID=cds.MS.gene41450.t1;Parent=MS.gene41450.t1 |
| chr3.1 | exon | 52328344 | 52328430 | 52328344 | ID=MS.gene41450.t1.exon4;Parent=MS.gene41450.t1 |
| chr3.1 | CDS | 52328344 | 52328430 | 52328344 | ID=cds.MS.gene41450.t1;Parent=MS.gene41450.t1 |
| chr3.1 | exon | 52328517 | 52328603 | 52328517 | ID=MS.gene41450.t1.exon5;Parent=MS.gene41450.t1 |
| chr3.1 | CDS | 52328517 | 52328603 | 52328517 | ID=cds.MS.gene41450.t1;Parent=MS.gene41450.t1 |
| chr3.1 | exon | 52329036 | 52329122 | 52329036 | ID=MS.gene41450.t1.exon6;Parent=MS.gene41450.t1 |
| chr3.1 | CDS | 52329036 | 52329122 | 52329036 | ID=cds.MS.gene41450.t1;Parent=MS.gene41450.t1 |
| chr3.1 | exon | 52329382 | 52329468 | 52329382 | ID=MS.gene41450.t1.exon7;Parent=MS.gene41450.t1 |
| chr3.1 | CDS | 52329382 | 52329468 | 52329382 | ID=cds.MS.gene41450.t1;Parent=MS.gene41450.t1 |
| chr3.1 | exon | 52329555 | 52329641 | 52329555 | ID=MS.gene41450.t1.exon8;Parent=MS.gene41450.t1 |
| chr3.1 | CDS | 52329555 | 52329641 | 52329555 | ID=cds.MS.gene41450.t1;Parent=MS.gene41450.t1 |
| chr3.1 | exon | 52329728 | 52329814 | 52329728 | ID=MS.gene41450.t1.exon9;Parent=MS.gene41450.t1 |
| chr3.1 | CDS | 52329728 | 52329814 | 52329728 | ID=cds.MS.gene41450.t1;Parent=MS.gene41450.t1 |
| chr3.1 | exon | 52330074 | 52330160 | 52330074 | ID=MS.gene41450.t1.exon10;Parent=MS.gene41450.t1 |
| chr3.1 | CDS | 52330074 | 52330160 | 52330074 | ID=cds.MS.gene41450.t1;Parent=MS.gene41450.t1 |
| chr3.1 | exon | 52330246 | 52330332 | 52330246 | ID=MS.gene41450.t1.exon11;Parent=MS.gene41450.t1 |
| chr3.1 | CDS | 52330246 | 52330332 | 52330246 | ID=cds.MS.gene41450.t1;Parent=MS.gene41450.t1 |
| chr3.1 | exon | 52330419 | 52330505 | 52330419 | ID=MS.gene41450.t1.exon12;Parent=MS.gene41450.t1 |
| chr3.1 | CDS | 52330419 | 52330505 | 52330419 | ID=cds.MS.gene41450.t1;Parent=MS.gene41450.t1 |
| chr3.1 | exon | 52330591 | 52330677 | 52330591 | ID=MS.gene41450.t1.exon13;Parent=MS.gene41450.t1 |
| chr3.1 | CDS | 52330591 | 52330677 | 52330591 | ID=cds.MS.gene41450.t1;Parent=MS.gene41450.t1 |
| chr3.1 | exon | 52330765 | 52330851 | 52330765 | ID=MS.gene41450.t1.exon14;Parent=MS.gene41450.t1 |
| chr3.1 | CDS | 52330765 | 52330851 | 52330765 | ID=cds.MS.gene41450.t1;Parent=MS.gene41450.t1 |
| chr3.1 | exon | 52331111 | 52331197 | 52331111 | ID=MS.gene41450.t1.exon15;Parent=MS.gene41450.t1 |
| chr3.1 | CDS | 52331111 | 52331197 | 52331111 | ID=cds.MS.gene41450.t1;Parent=MS.gene41450.t1 |
| chr3.1 | exon | 52331284 | 52331370 | 52331284 | ID=MS.gene41450.t1.exon16;Parent=MS.gene41450.t1 |
| chr3.1 | CDS | 52331284 | 52331370 | 52331284 | ID=cds.MS.gene41450.t1;Parent=MS.gene41450.t1 |
| chr3.1 | exon | 52331456 | 52331542 | 52331456 | ID=MS.gene41450.t1.exon17;Parent=MS.gene41450.t1 |
| chr3.1 | CDS | 52331456 | 52331542 | 52331456 | ID=cds.MS.gene41450.t1;Parent=MS.gene41450.t1 |
| chr3.1 | exon | 52331800 | 52331886 | 52331800 | ID=MS.gene41450.t1.exon18;Parent=MS.gene41450.t1 |
| chr3.1 | CDS | 52331800 | 52331886 | 52331800 | ID=cds.MS.gene41450.t1;Parent=MS.gene41450.t1 |
| chr3.1 | exon | 52331973 | 52332059 | 52331973 | ID=MS.gene41450.t1.exon19;Parent=MS.gene41450.t1 |
| chr3.1 | CDS | 52331973 | 52332059 | 52331973 | ID=cds.MS.gene41450.t1;Parent=MS.gene41450.t1 |
| chr3.1 | exon | 52332146 | 52332232 | 52332146 | ID=MS.gene41450.t1.exon20;Parent=MS.gene41450.t1 |
| chr3.1 | CDS | 52332146 | 52332232 | 52332146 | ID=cds.MS.gene41450.t1;Parent=MS.gene41450.t1 |
| chr3.1 | exon | 52332318 | 52332404 | 52332318 | ID=MS.gene41450.t1.exon21;Parent=MS.gene41450.t1 |
| chr3.1 | CDS | 52332318 | 52332404 | 52332318 | ID=cds.MS.gene41450.t1;Parent=MS.gene41450.t1 |
| chr3.1 | exon | 52332492 | 52332578 | 52332492 | ID=MS.gene41450.t1.exon22;Parent=MS.gene41450.t1 |
| chr3.1 | CDS | 52332492 | 52332578 | 52332492 | ID=cds.MS.gene41450.t1;Parent=MS.gene41450.t1 |
| chr3.1 | exon | 52332838 | 52332924 | 52332838 | ID=MS.gene41450.t1.exon23;Parent=MS.gene41450.t1 |
| chr3.1 | CDS | 52332838 | 52332924 | 52332838 | ID=cds.MS.gene41450.t1;Parent=MS.gene41450.t1 |
| chr3.1 | exon | 52333183 | 52333269 | 52333183 | ID=MS.gene41450.t1.exon24;Parent=MS.gene41450.t1 |
| chr3.1 | CDS | 52333183 | 52333269 | 52333183 | ID=cds.MS.gene41450.t1;Parent=MS.gene41450.t1 |
| chr3.1 | exon | 52333356 | 52333442 | 52333356 | ID=MS.gene41450.t1.exon25;Parent=MS.gene41450.t1 |
| chr3.1 | CDS | 52333356 | 52333442 | 52333356 | ID=cds.MS.gene41450.t1;Parent=MS.gene41450.t1 |
| chr3.1 | exon | 52333529 | 52333615 | 52333529 | ID=MS.gene41450.t1.exon26;Parent=MS.gene41450.t1 |
| chr3.1 | CDS | 52333529 | 52333615 | 52333529 | ID=cds.MS.gene41450.t1;Parent=MS.gene41450.t1 |
| chr3.1 | exon | 52334607 | 52334681 | 52334607 | ID=MS.gene41450.t1.exon27;Parent=MS.gene41450.t1 |
| chr3.1 | CDS | 52334607 | 52334681 | 52334607 | ID=cds.MS.gene41450.t1;Parent=MS.gene41450.t1 |
| Gene Sequence |
| Protein sequence |
