Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene41472.t1 | XP_004500366.1 | 81.2 | 208 | 37 | 1 | 1 | 206 | 373 | 580 | 3.80E-91 | 344.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene41472.t1 | Q4F883 | 40.3 | 191 | 107 | 3 | 6 | 190 | 337 | 526 | 3.0e-36 | 153.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene41472.t1 | A0A1S2Y842 | 81.2 | 208 | 37 | 1 | 1 | 206 | 373 | 580 | 2.7e-91 | 344.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049567 | MS.gene41472 | 0.808731 | 2.68E-50 | -1.69E-46 |
| MS.gene051207 | MS.gene41472 | 0.808058 | 3.73E-50 | -1.69E-46 |
| MS.gene051492 | MS.gene41472 | 0.801332 | 9.46E-49 | -1.69E-46 |
| MS.gene052208 | MS.gene41472 | 0.818771 | 1.65E-52 | -1.69E-46 |
| MS.gene053646 | MS.gene41472 | 0.804709 | 1.90E-49 | -1.69E-46 |
| MS.gene05415 | MS.gene41472 | 0.802586 | 5.23E-49 | -1.69E-46 |
| MS.gene057026 | MS.gene41472 | 0.817032 | 4.07E-52 | -1.69E-46 |
| MS.gene058477 | MS.gene41472 | 0.80316 | 3.98E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene41472.t1 | MTR_3g467360 | 79.310 | 203 | 40 | 1 | 6 | 206 | 379 | 581 | 1.97e-115 | 340 |
| MS.gene41472.t1 | MTR_3g467360 | 79.310 | 203 | 40 | 1 | 6 | 206 | 373 | 575 | 2.04e-115 | 340 |
| MS.gene41472.t1 | MTR_6g007975 | 71.429 | 203 | 56 | 1 | 6 | 206 | 375 | 577 | 1.56e-101 | 305 |
| MS.gene41472.t1 | MTR_6g007985 | 68.627 | 204 | 62 | 1 | 5 | 206 | 298 | 501 | 4.92e-99 | 296 |
| MS.gene41472.t1 | MTR_6g008015 | 68.627 | 204 | 62 | 1 | 5 | 206 | 375 | 578 | 2.66e-98 | 296 |
| MS.gene41472.t1 | MTR_6g008055 | 67.961 | 206 | 64 | 1 | 1 | 206 | 372 | 575 | 1.23e-96 | 292 |
| MS.gene41472.t1 | MTR_6g007980 | 58.373 | 209 | 83 | 3 | 1 | 205 | 367 | 575 | 5.48e-80 | 249 |
| MS.gene41472.t1 | MTR_6g007980 | 58.373 | 209 | 83 | 3 | 1 | 205 | 368 | 576 | 6.65e-80 | 249 |
| MS.gene41472.t1 | MTR_6g007980 | 58.373 | 209 | 83 | 3 | 1 | 205 | 369 | 577 | 6.80e-80 | 249 |
| MS.gene41472.t1 | MTR_0286s0040 | 61.828 | 186 | 45 | 2 | 23 | 206 | 226 | 387 | 3.23e-74 | 229 |
| MS.gene41472.t1 | MTR_3g467350 | 89.796 | 98 | 10 | 0 | 109 | 206 | 385 | 482 | 1.02e-57 | 189 |
| MS.gene41472.t1 | MTR_3g467350 | 68.421 | 38 | 12 | 0 | 1 | 38 | 351 | 388 | 3.50e-11 | 62.0 |
| MS.gene41472.t1 | MTR_8g494200 | 28.415 | 183 | 123 | 4 | 2 | 177 | 430 | 611 | 1.92e-15 | 74.7 |
| MS.gene41472.t1 | MTR_3g078633 | 29.091 | 165 | 88 | 6 | 15 | 170 | 455 | 599 | 2.93e-11 | 62.4 |
| MS.gene41472.t1 | MTR_3g078633 | 29.091 | 165 | 88 | 6 | 15 | 170 | 383 | 527 | 3.02e-11 | 62.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene41472.t1 | AT5G14930 | 40.314 | 191 | 107 | 3 | 6 | 190 | 337 | 526 | 6.38e-44 | 154 |
| MS.gene41472.t1 | AT3G52430 | 27.485 | 171 | 112 | 4 | 15 | 177 | 354 | 520 | 2.83e-12 | 65.5 |
Find 30 sgRNAs with CRISPR-Local
Find 41 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAACGAGTATAAAGCAATTA+AGG | 0.216540 | 3.1:-51975456 | MS.gene41472:CDS |
| TGACTACAGATTCTTGCTTT+TGG | 0.265156 | 3.1:-51975649 | MS.gene41472:CDS |
| AAGACTATGTTCATAATAAA+AGG | 0.271277 | 3.1:-51975766 | MS.gene41472:CDS |
| CACCATTTACTGGGAGAAAA+TGG | 0.274895 | 3.1:-51975918 | MS.gene41472:CDS |
| TTTCGTACCCGTTGGTTATA+TGG | 0.289900 | 3.1:-51975854 | MS.gene41472:CDS |
| TATAGCATACAGCTTAATAT+TGG | 0.306524 | 3.1:+51975388 | None:intergenic |
| AAAGAAATTATTTGAATTTG+AGG | 0.327326 | 3.1:-51975558 | MS.gene41472:CDS |
| TATATGGTGGGACTACTTAC+AGG | 0.360221 | 3.1:-51975838 | MS.gene41472:CDS |
| AATATTAAGCTGTATGCTAT+AGG | 0.416242 | 3.1:-51975386 | MS.gene41472:CDS |
| GTATTGTTTGTATCACTTGT+TGG | 0.422070 | 3.1:+51975698 | None:intergenic |
| AACGAGTATAAAGCAATTAA+GGG | 0.451013 | 3.1:-51975455 | MS.gene41472:CDS |
| ACAGTTGGAGGAATGGTTGA+TGG | 0.465873 | 3.1:-51975729 | MS.gene41472:CDS |
| GTCTAAACACTTCAAACAGT+TGG | 0.484439 | 3.1:-51975744 | MS.gene41472:CDS |
| ATTGCTGAATACTATAGAAA+AGG | 0.495339 | 3.1:-51975794 | MS.gene41472:CDS |
| CGTACCCGTTGGTTATATGG+TGG | 0.499140 | 3.1:-51975851 | MS.gene41472:CDS |
| ACTTCAAACAGTTGGAGGAA+TGG | 0.506869 | 3.1:-51975736 | MS.gene41472:CDS |
| GTACCCGTTGGTTATATGGT+GGG | 0.534161 | 3.1:-51975850 | MS.gene41472:CDS |
| CACAAAGCAGCTATATTCGT+TGG | 0.545547 | 3.1:-51975481 | MS.gene41472:CDS |
| AGTCCCACCATATAACCAAC+GGG | 0.547594 | 3.1:+51975847 | None:intergenic |
| TAAACACTTCAAACAGTTGG+AGG | 0.552501 | 3.1:-51975741 | MS.gene41472:CDS |
| CAGTTGGAGGAATGGTTGAT+GGG | 0.574475 | 3.1:-51975728 | MS.gene41472:CDS |
| TGGGACTACTTACAGGAGAA+TGG | 0.583573 | 3.1:-51975831 | MS.gene41472:CDS |
| GTATGTTTATAAGTTGCTCA+AGG | 0.585546 | 3.1:-51975534 | MS.gene41472:CDS |
| AAAGCAGCTATATTCGTTGG+TGG | 0.600841 | 3.1:-51975478 | MS.gene41472:CDS |
| CAATACACGTAGAAAGAATG+TGG | 0.608038 | 3.1:-51975681 | MS.gene41472:CDS |
| TATGGAACTCAGTAACATCA+CGG | 0.622674 | 3.1:+51975948 | None:intergenic |
| TAGTCCCACCATATAACCAA+CGG | 0.625409 | 3.1:+51975846 | None:intergenic |
| GCTGAATACTATAGAAAAGG+CGG | 0.641083 | 3.1:-51975791 | MS.gene41472:CDS |
| TAGTATTCAGCAATAGCTAG+AGG | 0.652060 | 3.1:+51975803 | None:intergenic |
| TACACGTAGAAAGAATGTGG+AGG | 0.709499 | 3.1:-51975678 | MS.gene41472:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAGAAATTATTTGAATTTG+AGG | - | chr3.1:51975774-51975793 | MS.gene41472:CDS | 15.0% |
| !! | AAGACTATGTTCATAATAAA+AGG | - | chr3.1:51975566-51975585 | MS.gene41472:CDS | 20.0% |
| ! | AACGAGTATAAAGCAATTAA+GGG | - | chr3.1:51975877-51975896 | MS.gene41472:CDS | 25.0% |
| ! | AATATTAAGCTGTATGCTAT+AGG | - | chr3.1:51975946-51975965 | MS.gene41472:CDS | 25.0% |
| ! | ATTGCTGAATACTATAGAAA+AGG | - | chr3.1:51975538-51975557 | MS.gene41472:CDS | 25.0% |
| ! | TATAGCATACAGCTTAATAT+TGG | + | chr3.1:51975947-51975966 | None:intergenic | 25.0% |
| ATAAAAAGCTCACCATTTAC+TGG | - | chr3.1:51975404-51975423 | MS.gene41472:CDS | 30.0% | |
| CTTTGTGCTAGAAAAATATC+TGG | + | chr3.1:51975838-51975857 | None:intergenic | 30.0% | |
| GAACGAGTATAAAGCAATTA+AGG | - | chr3.1:51975876-51975895 | MS.gene41472:CDS | 30.0% | |
| GTATGTTTATAAGTTGCTCA+AGG | - | chr3.1:51975798-51975817 | MS.gene41472:CDS | 30.0% | |
| GTATTGTTTGTATCACTTGT+TGG | + | chr3.1:51975637-51975656 | None:intergenic | 30.0% | |
| TAAAAAGCTCACCATTTACT+GGG | - | chr3.1:51975405-51975424 | MS.gene41472:CDS | 30.0% | |
| ! | ACTTTTATGCAGAGAGTTAA+AGG | - | chr3.1:51975726-51975745 | MS.gene41472:CDS | 30.0% |
| ! | AGTAAATGGTGAGCTTTTTA+TGG | + | chr3.1:51975405-51975424 | None:intergenic | 30.0% |
| CAATACACGTAGAAAGAATG+TGG | - | chr3.1:51975651-51975670 | MS.gene41472:CDS | 35.0% | |
| CAGATGAAACCTCAAAAAGA+AGG | - | chr3.1:51975448-51975467 | MS.gene41472:CDS | 35.0% | |
| GCTGAATACTATAGAAAAGG+CGG | - | chr3.1:51975541-51975560 | MS.gene41472:CDS | 35.0% | |
| GTCTAAACACTTCAAACAGT+TGG | - | chr3.1:51975588-51975607 | MS.gene41472:CDS | 35.0% | |
| TAAACACTTCAAACAGTTGG+AGG | - | chr3.1:51975591-51975610 | MS.gene41472:CDS | 35.0% | |
| TAGTATTCAGCAATAGCTAG+AGG | + | chr3.1:51975532-51975551 | None:intergenic | 35.0% | |
| TATGGAACTCAGTAACATCA+CGG | + | chr3.1:51975387-51975406 | None:intergenic | 35.0% | |
| ! | AACCATTTTCTCCCAGTAAA+TGG | + | chr3.1:51975419-51975438 | None:intergenic | 35.0% |
| ! | TGACTACAGATTCTTGCTTT+TGG | - | chr3.1:51975683-51975702 | MS.gene41472:CDS | 35.0% |
| !! | GACTACAGATTCTTGCTTTT+GGG | - | chr3.1:51975684-51975703 | MS.gene41472:CDS | 35.0% |
| AAAGCAGCTATATTCGTTGG+TGG | - | chr3.1:51975854-51975873 | MS.gene41472:CDS | 40.0% | |
| ACTTCAAACAGTTGGAGGAA+TGG | - | chr3.1:51975596-51975615 | MS.gene41472:CDS | 40.0% | |
| CACAAAGCAGCTATATTCGT+TGG | - | chr3.1:51975851-51975870 | MS.gene41472:CDS | 40.0% | |
| CACCATTTACTGGGAGAAAA+TGG | - | chr3.1:51975414-51975433 | MS.gene41472:CDS | 40.0% | |
| TACACGTAGAAAGAATGTGG+AGG | - | chr3.1:51975654-51975673 | MS.gene41472:CDS | 40.0% | |
| TAGTCCCACCATATAACCAA+CGG | + | chr3.1:51975489-51975508 | None:intergenic | 40.0% | |
| ! | AAAGCTGCACCTTCTTTTTG+AGG | + | chr3.1:51975460-51975479 | None:intergenic | 40.0% |
| ! | TATATGGTGGGACTACTTAC+AGG | - | chr3.1:51975494-51975513 | MS.gene41472:CDS | 40.0% |
| ! | TTTCGTACCCGTTGGTTATA+TGG | - | chr3.1:51975478-51975497 | MS.gene41472:CDS | 40.0% |
| ACAGTTGGAGGAATGGTTGA+TGG | - | chr3.1:51975603-51975622 | MS.gene41472:CDS | 45.0% | |
| AGTCCCACCATATAACCAAC+GGG | + | chr3.1:51975488-51975507 | None:intergenic | 45.0% | |
| CAGTTGGAGGAATGGTTGAT+GGG | - | chr3.1:51975604-51975623 | MS.gene41472:CDS | 45.0% | |
| TGGGACTACTTACAGGAGAA+TGG | - | chr3.1:51975501-51975520 | MS.gene41472:CDS | 45.0% | |
| ! | CTTTTGGGCACATGTTGAAG+AGG | - | chr3.1:51975699-51975718 | MS.gene41472:CDS | 45.0% |
| ! | GTACCCGTTGGTTATATGGT+GGG | - | chr3.1:51975482-51975501 | MS.gene41472:CDS | 45.0% |
| ! | CGTACCCGTTGGTTATATGG+TGG | - | chr3.1:51975481-51975500 | MS.gene41472:CDS | 50.0% |
| ! | GTGCAGCTTTTCGTACCCGT+TGG | - | chr3.1:51975470-51975489 | MS.gene41472:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 51975367 | 51975987 | 51975367 | ID=MS.gene41472 |
| chr3.1 | mRNA | 51975367 | 51975987 | 51975367 | ID=MS.gene41472.t1;Parent=MS.gene41472 |
| chr3.1 | exon | 51975367 | 51975987 | 51975367 | ID=MS.gene41472.t1.exon1;Parent=MS.gene41472.t1 |
| chr3.1 | CDS | 51975367 | 51975987 | 51975367 | ID=cds.MS.gene41472.t1;Parent=MS.gene41472.t1 |
| Gene Sequence |
| Protein sequence |
