Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene41805.t1 | XP_003624720.1 | 85.2 | 338 | 8 | 1 | 52 | 347 | 61 | 398 | 8.70E-165 | 589.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene41805.t1 | Q9FLD7 | 47.9 | 336 | 132 | 2 | 55 | 347 | 111 | 446 | 4.3e-88 | 326.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene41805.t1 | G7L1K4 | 85.2 | 338 | 8 | 1 | 52 | 347 | 61 | 398 | 6.2e-165 | 589.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049142 | MS.gene41805 | 0.805414 | 1.35E-49 | -1.69E-46 |
| MS.gene050551 | MS.gene41805 | 0.831306 | 1.80E-55 | -1.69E-46 |
| MS.gene051029 | MS.gene41805 | 0.805249 | 1.46E-49 | -1.69E-46 |
| MS.gene052609 | MS.gene41805 | 0.807805 | 4.22E-50 | -1.69E-46 |
| MS.gene055465 | MS.gene41805 | 0.830572 | 2.72E-55 | -1.69E-46 |
| MS.gene055469 | MS.gene41805 | 0.801982 | 6.96E-49 | -1.69E-46 |
| MS.gene057915 | MS.gene41805 | 0.800435 | 1.44E-48 | -1.69E-46 |
| MS.gene058087 | MS.gene41805 | 0.812005 | 5.27E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene41805.t1 | MTR_7g086720 | 85.207 | 338 | 8 | 1 | 52 | 347 | 61 | 398 | 0.0 | 590 |
| MS.gene41805.t1 | MTR_6g027550 | 64.873 | 316 | 66 | 2 | 77 | 347 | 1 | 316 | 3.62e-151 | 428 |
| MS.gene41805.t1 | MTR_0072s0020 | 49.853 | 339 | 119 | 8 | 55 | 347 | 99 | 432 | 3.67e-108 | 323 |
| MS.gene41805.t1 | MTR_6g011080 | 47.198 | 339 | 136 | 2 | 52 | 347 | 94 | 432 | 6.42e-106 | 317 |
| MS.gene41805.t1 | MTR_2g085300 | 47.788 | 339 | 129 | 5 | 52 | 347 | 90 | 423 | 6.47e-103 | 309 |
| MS.gene41805.t1 | MTR_4g022240 | 45.536 | 336 | 140 | 2 | 55 | 347 | 83 | 418 | 4.04e-102 | 307 |
| MS.gene41805.t1 | MTR_4g114140 | 49.153 | 295 | 107 | 2 | 55 | 306 | 85 | 379 | 2.77e-98 | 296 |
| MS.gene41805.t1 | MTR_0662s0030 | 49.057 | 265 | 92 | 2 | 85 | 306 | 16 | 280 | 4.58e-88 | 266 |
| MS.gene41805.t1 | MTR_1g108280 | 39.714 | 350 | 141 | 5 | 55 | 347 | 60 | 396 | 5.49e-78 | 244 |
| MS.gene41805.t1 | MTR_1g046740 | 37.168 | 339 | 167 | 4 | 52 | 347 | 103 | 438 | 3.49e-72 | 231 |
| MS.gene41805.t1 | MTR_4g008010 | 35.608 | 337 | 172 | 4 | 55 | 347 | 71 | 406 | 1.12e-63 | 208 |
| MS.gene41805.t1 | MTR_4g036245 | 42.623 | 61 | 35 | 0 | 50 | 110 | 4 | 64 | 8.01e-11 | 61.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene41805.t1 | AT3G03690 | 62.857 | 280 | 62 | 1 | 52 | 289 | 61 | 340 | 2.29e-125 | 365 |
| MS.gene41805.t1 | AT4G27480 | 47.090 | 378 | 149 | 5 | 13 | 347 | 51 | 420 | 2.34e-114 | 338 |
| MS.gene41805.t1 | AT4G27480 | 47.090 | 378 | 149 | 5 | 13 | 347 | 51 | 420 | 2.34e-114 | 338 |
| MS.gene41805.t1 | AT4G27480 | 47.090 | 378 | 149 | 5 | 13 | 347 | 51 | 420 | 2.34e-114 | 338 |
| MS.gene41805.t1 | AT1G03520 | 49.408 | 338 | 122 | 7 | 55 | 347 | 24 | 357 | 1.32e-108 | 322 |
| MS.gene41805.t1 | AT4G03340 | 49.112 | 338 | 123 | 7 | 55 | 347 | 114 | 447 | 3.47e-108 | 323 |
| MS.gene41805.t1 | AT5G39990 | 47.917 | 336 | 132 | 2 | 55 | 347 | 111 | 446 | 1.25e-107 | 322 |
| MS.gene41805.t1 | AT1G03520 | 49.408 | 338 | 122 | 7 | 55 | 347 | 113 | 446 | 1.44e-107 | 322 |
| MS.gene41805.t1 | AT1G53100 | 48.817 | 338 | 126 | 6 | 52 | 347 | 90 | 422 | 1.90e-107 | 321 |
| MS.gene41805.t1 | AT3G15350 | 49.267 | 341 | 123 | 6 | 52 | 347 | 88 | 423 | 4.02e-107 | 320 |
| MS.gene41805.t1 | AT3G15350 | 49.267 | 341 | 123 | 6 | 52 | 347 | 88 | 423 | 4.02e-107 | 320 |
| MS.gene41805.t1 | AT1G53100 | 50.154 | 325 | 115 | 6 | 52 | 334 | 90 | 409 | 1.43e-105 | 316 |
| MS.gene41805.t1 | AT5G15050 | 46.903 | 339 | 137 | 2 | 52 | 347 | 95 | 433 | 1.23e-104 | 314 |
| MS.gene41805.t1 | AT4G03340 | 45.266 | 338 | 113 | 8 | 55 | 347 | 114 | 424 | 1.15e-92 | 283 |
| MS.gene41805.t1 | AT2G37585 | 41.964 | 336 | 131 | 5 | 55 | 347 | 69 | 383 | 2.04e-86 | 266 |
| MS.gene41805.t1 | AT1G71070 | 38.757 | 338 | 160 | 4 | 55 | 347 | 59 | 394 | 1.87e-77 | 243 |
| MS.gene41805.t1 | AT3G24040 | 35.294 | 340 | 175 | 4 | 52 | 347 | 76 | 414 | 3.12e-70 | 225 |
| MS.gene41805.t1 | AT3G03690 | 71.654 | 127 | 36 | 0 | 52 | 178 | 61 | 187 | 1.39e-61 | 196 |
Find 69 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCTCTATAAGTTACCAAATT+AGG | 0.185633 | 7.4:+20943463 | None:intergenic |
| TTCGGCTACTGTTGCTGTTT+TGG | 0.185640 | 7.4:+20944924 | None:intergenic |
| TGAATCAAATAGTAGTTATT+TGG | 0.198310 | 7.4:+20943589 | None:intergenic |
| GATTGTTGGGAAGCCTAATT+TGG | 0.234769 | 7.4:-20943476 | MS.gene41805:CDS |
| TATTTGATTCACATGGATTA+TGG | 0.248998 | 7.4:-20943577 | MS.gene41805:CDS |
| CAGCTTGGGTGCTGTGGCTT+TGG | 0.252990 | 7.4:+20944992 | None:intergenic |
| ACTTAAAGATTATAGGAAAA+TGG | 0.276934 | 7.4:-20926909 | MS.gene41805:CDS |
| TTGCTGGTTCTGGTGTTGTA+TGG | 0.287129 | 7.4:+20945038 | None:intergenic |
| TGCTGGTTCTGGTGTTGTAT+GGG | 0.291430 | 7.4:+20945039 | None:intergenic |
| CCAGCTGCGGCTGTTATTGC+TGG | 0.329139 | 7.4:+20945022 | None:intergenic |
| TTTGTTAAACAAAATCCTTA+TGG | 0.342506 | 7.4:-20926678 | MS.gene41805:CDS |
| AGAGACCTAGGATGTTGTTT+TGG | 0.350611 | 7.4:+20926935 | None:intergenic |
| CTGTTTACCTACACTGCATT+TGG | 0.352059 | 7.4:+20926642 | None:intergenic |
| ACATCTTTATGTTCTGCATC+TGG | 0.364339 | 7.4:+20943550 | None:intergenic |
| GTTGGAAATGTTTGGATTGT+TGG | 0.368166 | 7.4:-20943490 | MS.gene41805:CDS |
| TTAGTCAAGTTGGAAATGTT+TGG | 0.374364 | 7.4:-20943498 | MS.gene41805:CDS |
| CCTAATTTGGTAACTTATAG+AGG | 0.376429 | 7.4:-20943463 | MS.gene41805:CDS |
| GGTTTGAGAACTGAGAATTA+TGG | 0.376756 | 7.4:-20926740 | MS.gene41805:CDS |
| TTTGGGGCTGCTGGTGGGAT+GGG | 0.378800 | 7.4:+20944942 | None:intergenic |
| ACTGTTTGGAAATATCCTTC+TGG | 0.379198 | 7.4:+20927031 | None:intergenic |
| GCGGCTGTTATTGCTGGTTC+TGG | 0.379845 | 7.4:+20945028 | None:intergenic |
| CTCTAGGACTTAAAGATTAT+AGG | 0.380227 | 7.4:-20926916 | MS.gene41805:CDS |
| ATGATCTTCACTACATTACT+TGG | 0.398716 | 7.4:-20926967 | MS.gene41805:CDS |
| AACAAGAGTGGCTGTGCTGC+TGG | 0.400016 | 7.4:-20945067 | MS.gene41805:CDS |
| AGCCTTCTTGAGCCAGGACC+AGG | 0.423280 | 7.4:+20926707 | None:intergenic |
| TGCTTCTTAAGACATGTCAT+TGG | 0.425258 | 7.4:-20943402 | MS.gene41805:CDS |
| TTAAGACATGTCATTGGGAT+TGG | 0.429628 | 7.4:-20943396 | MS.gene41805:CDS |
| TTTGGAGGGTGGTGTTCTCA+AGG | 0.438791 | 7.4:-20926785 | MS.gene41805:CDS |
| TTGGAAATGTTTGGATTGTT+GGG | 0.441038 | 7.4:-20943489 | MS.gene41805:CDS |
| GGTTTGCTTAAACCTGGTCC+TGG | 0.447605 | 7.4:-20926719 | MS.gene41805:CDS |
| GCTTCTTAAGACATGTCATT+GGG | 0.449821 | 7.4:-20943401 | MS.gene41805:CDS |
| GAGATACTGTGAAGCTCAAA+AGG | 0.453050 | 7.4:-20943633 | MS.gene41805:CDS |
| CTGGTTCTGGTGTTGTATGG+GGG | 0.455617 | 7.4:+20945041 | None:intergenic |
| GCTGGTTCTGGTGTTGTATG+GGG | 0.475074 | 7.4:+20945040 | None:intergenic |
| GTGAGTTGCATATTACTGTT+TGG | 0.477123 | 7.4:+20927017 | None:intergenic |
| CTGAGTATTGCATCGTGGGA+TGG | 0.479349 | 7.4:-20927105 | MS.gene41805:CDS |
| ATACAACATAGTAGTCGCTT+GGG | 0.482462 | 7.4:-20943014 | MS.gene41805:intron |
| TAGTGCTTCTGATTATCCCT+TGG | 0.482480 | 7.4:-20943362 | MS.gene41805:CDS |
| TATCCCTTGGTTACACAAGA+TGG | 0.484610 | 7.4:-20943349 | MS.gene41805:intron |
| CATACAACATAGTAGTCGCT+TGG | 0.491200 | 7.4:-20943015 | MS.gene41805:CDS |
| CTGCAGCAGCTTGGGTGCTG+TGG | 0.492773 | 7.4:+20944986 | None:intergenic |
| AGAGTAGTTGCAAGCATAGT+TGG | 0.496093 | 7.4:+20943439 | None:intergenic |
| TGAGTATTGCATCGTGGGAT+GGG | 0.496769 | 7.4:-20927104 | MS.gene41805:CDS |
| AAACAACATCCTAGGTCTCT+AGG | 0.497584 | 7.4:-20926932 | MS.gene41805:CDS |
| AATTATGGTTTGCTTAAACC+TGG | 0.512607 | 7.4:-20926725 | MS.gene41805:CDS |
| TAGTAGAGGAGTAGAGTCCT+TGG | 0.516432 | 7.4:+20927073 | None:intergenic |
| AGACAAAGTTAGTGTAGTAG+AGG | 0.518512 | 7.4:+20927059 | None:intergenic |
| TGCAACTACTCTTCATGCTA+TGG | 0.532994 | 7.4:-20943428 | MS.gene41805:CDS |
| TGTATGAAATTAATATCCCT+TGG | 0.534880 | 7.4:+20943032 | None:intergenic |
| GGAAAGAACAAAGCATGCTC+AGG | 0.540430 | 7.4:-20926761 | MS.gene41805:CDS |
| ATCCTCCAAAACAACATCCT+AGG | 0.540904 | 7.4:-20926940 | MS.gene41805:CDS |
| CACAAAATTCAGGTTCAGCA+TGG | 0.562912 | 7.4:-20927150 | MS.gene41805:intron |
| TGGTTCTGGTGTTGTATGGG+GGG | 0.565121 | 7.4:+20945042 | None:intergenic |
| TCTTTAAGTCCTAGAGACCT+AGG | 0.565974 | 7.4:+20926923 | None:intergenic |
| AATGTAGTGAAGATCATGGT+TGG | 0.568469 | 7.4:+20926973 | None:intergenic |
| TAACTACTATTTGATTCACA+TGG | 0.570348 | 7.4:-20943584 | MS.gene41805:CDS |
| CCAGCAATAACAGCCGCAGC+TGG | 0.576192 | 7.4:-20945022 | MS.gene41805:CDS |
| TGATCTTCACTACATTACTT+GGG | 0.576959 | 7.4:-20926966 | MS.gene41805:CDS |
| AAGTAATGTAGTGAAGATCA+TGG | 0.594031 | 7.4:+20926969 | None:intergenic |
| AACCTGGTCCTGGCTCAAGA+AGG | 0.597108 | 7.4:-20926709 | MS.gene41805:CDS |
| GTGAAGATCATGGTTGGCAG+TGG | 0.602943 | 7.4:+20926979 | None:intergenic |
| AACTTTGTCTCATCACCAGA+AGG | 0.613473 | 7.4:-20927046 | MS.gene41805:CDS |
| AGGAACTAAATGAACAAGAG+TGG | 0.628841 | 7.4:-20945079 | None:intergenic |
| CATACCATCTTGTGTAACCA+AGG | 0.634114 | 7.4:+20943345 | None:intergenic |
| TGGGATGGGAAAATCTGCCA+AGG | 0.635256 | 7.4:-20927090 | MS.gene41805:CDS |
| GGAGGGTGGTGTTCTCAAGG+TGG | 0.638629 | 7.4:-20926782 | MS.gene41805:CDS |
| AGATGCAGAACATAAAGATG+TGG | 0.654179 | 7.4:-20943548 | MS.gene41805:CDS |
| ATACCATCTTGTGTAACCAA+GGG | 0.666206 | 7.4:+20943346 | None:intergenic |
| CTTTGGTGTATGACCAGCTG+CGG | 0.689035 | 7.4:+20945009 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 20926650 | 20945092 | 20926650 | ID=MS.gene41805 |
| chr7.4 | mRNA | 20926650 | 20945092 | 20926650 | ID=MS.gene41805.t1;Parent=MS.gene41805 |
| chr7.4 | exon | 20944933 | 20945092 | 20944933 | ID=MS.gene41805.t1.exon1;Parent=MS.gene41805.t1 |
| chr7.4 | CDS | 20944933 | 20945092 | 20944933 | ID=cds.MS.gene41805.t1;Parent=MS.gene41805.t1 |
| chr7.4 | exon | 20943350 | 20943655 | 20943350 | ID=MS.gene41805.t1.exon2;Parent=MS.gene41805.t1 |
| chr7.4 | CDS | 20943350 | 20943655 | 20943350 | ID=cds.MS.gene41805.t1;Parent=MS.gene41805.t1 |
| chr7.4 | exon | 20943015 | 20943080 | 20943015 | ID=MS.gene41805.t1.exon3;Parent=MS.gene41805.t1 |
| chr7.4 | CDS | 20943015 | 20943080 | 20943015 | ID=cds.MS.gene41805.t1;Parent=MS.gene41805.t1 |
| chr7.4 | exon | 20926650 | 20927160 | 20926650 | ID=MS.gene41805.t1.exon4;Parent=MS.gene41805.t1 |
| chr7.4 | CDS | 20926650 | 20927160 | 20926650 | ID=cds.MS.gene41805.t1;Parent=MS.gene41805.t1 |
| Gene Sequence |
| Protein sequence |
