Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene42019.t1 | XP_013452022.1 | 99 | 195 | 2 | 0 | 137 | 331 | 1 | 195 | 4.20E-100 | 374.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene42019.t1 | P34788 | 88.9 | 117 | 13 | 0 | 1 | 117 | 1 | 117 | 7.8e-55 | 215.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene42019.t1 | G7IEW6 | 99.0 | 195 | 2 | 0 | 137 | 331 | 1 | 195 | 3.0e-100 | 374.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049306 | MS.gene42019 | 0.82539 | 4.81E-54 | -1.69E-46 |
| MS.gene051927 | MS.gene42019 | 0.810581 | 1.07E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene42019.t1 | MTR_6g043220 | 98.974 | 195 | 2 | 0 | 137 | 331 | 1 | 195 | 5.09e-143 | 402 |
| MS.gene42019.t1 | MTR_6g043220 | 96.575 | 146 | 5 | 0 | 186 | 331 | 45 | 190 | 1.01e-101 | 297 |
| MS.gene42019.t1 | MTR_1g098250 | 63.810 | 210 | 58 | 3 | 137 | 331 | 1 | 207 | 1.84e-85 | 256 |
| MS.gene42019.t1 | MTR_6g043210 | 80.263 | 152 | 19 | 1 | 1 | 141 | 1 | 152 | 7.69e-83 | 248 |
| MS.gene42019.t1 | MTR_1g098170 | 78.947 | 152 | 21 | 1 | 1 | 141 | 1 | 152 | 1.44e-81 | 244 |
| MS.gene42019.t1 | MTR_1g098220 | 78.947 | 152 | 21 | 1 | 1 | 141 | 1 | 152 | 1.44e-81 | 244 |
| MS.gene42019.t1 | MTR_1g098220 | 90.625 | 128 | 12 | 0 | 1 | 128 | 1 | 128 | 1.71e-81 | 243 |
| MS.gene42019.t1 | MTR_1g492710 | 95.726 | 117 | 5 | 0 | 1 | 117 | 794 | 910 | 2.21e-71 | 239 |
| MS.gene42019.t1 | MTR_8g092210 | 50.237 | 211 | 82 | 5 | 137 | 331 | 1 | 204 | 5.13e-59 | 189 |
| MS.gene42019.t1 | MTR_5g027650 | 92.405 | 79 | 6 | 0 | 39 | 117 | 11 | 89 | 6.81e-47 | 154 |
| MS.gene42019.t1 | MTR_5g027580 | 91.139 | 79 | 7 | 0 | 39 | 117 | 48 | 126 | 3.27e-46 | 154 |
| MS.gene42019.t1 | MTR_5g027670 | 92.424 | 66 | 5 | 0 | 52 | 117 | 1 | 66 | 5.74e-38 | 130 |
| MS.gene42019.t1 | MTR_5g027615 | 92.424 | 66 | 5 | 0 | 52 | 117 | 1 | 66 | 2.54e-36 | 126 |
| MS.gene42019.t1 | MTR_5g027605 | 92.424 | 66 | 5 | 0 | 52 | 117 | 1 | 66 | 2.54e-36 | 126 |
| MS.gene42019.t1 | MTR_5g027640 | 92.424 | 66 | 5 | 0 | 52 | 117 | 1 | 66 | 2.54e-36 | 126 |
| MS.gene42019.t1 | MTR_5g027600 | 92.424 | 66 | 5 | 0 | 52 | 117 | 1 | 66 | 2.54e-36 | 126 |
| MS.gene42019.t1 | MTR_1005s0010 | 92.063 | 63 | 5 | 0 | 55 | 117 | 2 | 64 | 6.57e-35 | 122 |
| MS.gene42019.t1 | MTR_4g015960 | 90.000 | 50 | 5 | 0 | 67 | 116 | 9 | 58 | 2.46e-23 | 100 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene42019.t1 | AT4G09800 | 71.711 | 152 | 32 | 1 | 1 | 141 | 1 | 152 | 1.24e-74 | 227 |
| MS.gene42019.t1 | AT1G34030 | 71.711 | 152 | 32 | 1 | 1 | 141 | 1 | 152 | 1.24e-74 | 227 |
| MS.gene42019.t1 | AT1G22780 | 71.711 | 152 | 32 | 1 | 1 | 141 | 1 | 152 | 1.24e-74 | 227 |
| MS.gene42019.t1 | AT4G09830 | 62.626 | 198 | 64 | 3 | 137 | 331 | 1 | 191 | 2.37e-70 | 217 |
| MS.gene42019.t1 | AT5G64780 | 46.961 | 181 | 83 | 3 | 137 | 314 | 1 | 171 | 1.19e-43 | 148 |
Find 80 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AATCTTCAGCTTTATCAAAT+TGG | 0.213955 | 6.4:+16871311 | MS.gene42019:CDS |
| CCAGTCACTTCAGGATCTTC+TGG | 0.220585 | 6.4:+16871185 | MS.gene42019:CDS |
| TCAGGCTTTCCAGTCACTTC+AGG | 0.296519 | 6.4:+16871176 | MS.gene42019:CDS |
| ATCCATGGCAGAATCATTAT+TGG | 0.297537 | 6.4:-16871393 | None:intergenic |
| TCAAGTTGTTTCAAATCAAC+TGG | 0.336957 | 6.4:+16860043 | MS.gene42019:CDS |
| CATGAAGTTGAGGGATGATT+TGG | 0.348922 | 6.4:+16860067 | MS.gene42019:CDS |
| AGCTGAAGATTTCGACGAAC+AGG | 0.349902 | 6.4:-16871299 | None:intergenic |
| CGTTTCTTCGCCATTGGAAT+CGG | 0.355485 | 6.4:-16865200 | None:intergenic |
| CGAAAGCTTCTGATGATTCA+TGG | 0.380448 | 6.4:-16865318 | None:intergenic |
| ATGATGCCTGTCACCAGTTC+AGG | 0.381654 | 6.4:+16871158 | MS.gene42019:CDS |
| TGATGTAGTTAACTACATTC+AGG | 0.382450 | 6.4:+16868104 | MS.gene42019:CDS |
| TCTTCTGGCCAAACGATAGC+TGG | 0.384017 | 6.4:+16871200 | MS.gene42019:CDS |
| GCATCATTGGTTGTGGCTGC+TGG | 0.392642 | 6.4:-16871142 | None:intergenic |
| AATGAGGGTTGTTGTTGATT+CGG | 0.397849 | 6.4:-16865154 | None:intergenic |
| ATGACTTCAATCAAAGGTAT+TGG | 0.407492 | 6.4:+16858969 | MS.gene42019:CDS |
| GCCCGGCCCCAGCTATCGTT+TGG | 0.407559 | 6.4:-16871208 | None:intergenic |
| GACTTGCGTTTCTTCGCCAT+TGG | 0.418361 | 6.4:-16865206 | None:intergenic |
| GGCAATTCAAGGTCCCTGAT+TGG | 0.439048 | 6.4:+16859979 | MS.gene42019:CDS |
| TTTGAAAACACCGAGTTCTT+CGG | 0.439137 | 6.4:-16871263 | None:intergenic |
| CAGAATGAGTTAGATTATTG+TGG | 0.452182 | 6.4:+16871080 | MS.gene42019:intron |
| ATCAGAGCGGAGTCCTTGCC+CGG | 0.458881 | 6.4:-16871225 | None:intergenic |
| CGGTGGTGGATGGAGGAATG+AGG | 0.471918 | 6.4:-16865170 | None:intergenic |
| GGCCAAACGATAGCTGGGGC+CGG | 0.480085 | 6.4:+16871206 | MS.gene42019:CDS |
| TGGAGCGCTTGAAGAAGATC+AGG | 0.487727 | 6.4:+16860087 | MS.gene42019:CDS |
| GCCAAACGATAGCTGGGGCC+GGG | 0.491569 | 6.4:+16871207 | MS.gene42019:CDS |
| CAGAAGCTTTCGTTTCATGC+TGG | 0.492228 | 6.4:+16865329 | MS.gene42019:CDS |
| GAACTGGTGACAGGCATCAT+TGG | 0.493043 | 6.4:-16871155 | None:intergenic |
| AGAATTAGCAGCGGTGGAGA+AGG | 0.493812 | 6.4:-16865273 | None:intergenic |
| ACGATAGCTGGGGCCGGGCA+AGG | 0.495454 | 6.4:+16871212 | MS.gene42019:CDS |
| CGGCGGAGATCGGTGGTGGA+TGG | 0.497980 | 6.4:-16865180 | None:intergenic |
| GGTGCTCTGGGTGACATGGA+TGG | 0.499547 | 6.4:-16871110 | None:intergenic |
| CCATTGGAATCGGCGGAGAT+CGG | 0.499729 | 6.4:-16865190 | None:intergenic |
| CGGTGGTGCTCTGGGTGACA+TGG | 0.500668 | 6.4:-16871114 | None:intergenic |
| AGTGACTGGAAAGCCTGAAC+TGG | 0.501227 | 6.4:-16871171 | None:intergenic |
| GTGTTGAATACCAACGTTGA+TGG | 0.503977 | 6.4:+16858924 | MS.gene42019:CDS |
| GACCTTGAATTGCCTAGGGT+TGG | 0.508321 | 6.4:-16859970 | None:intergenic |
| CGGAGATCGGTGGTGGATGG+AGG | 0.516588 | 6.4:-16865177 | None:intergenic |
| CTTCTGGCCAAACGATAGCT+GGG | 0.517507 | 6.4:+16871201 | MS.gene42019:CDS |
| TGAATTGACTGCCGCTGAGT+TGG | 0.517580 | 6.4:+16859926 | MS.gene42019:CDS |
| GAATCGGCGGAGATCGGTGG+TGG | 0.522126 | 6.4:-16865184 | None:intergenic |
| GAACAGAAAGAAGGATTACA+AGG | 0.525479 | 6.4:+16860007 | MS.gene42019:CDS |
| TGCCAACCCTAGGCAATTCA+AGG | 0.526783 | 6.4:+16859968 | MS.gene42019:CDS |
| AGAAAGAGAATTAGCAGCGG+TGG | 0.534056 | 6.4:-16865279 | None:intergenic |
| GGTGGTGGATGGAGGAATGA+GGG | 0.535280 | 6.4:-16865169 | None:intergenic |
| GGAAAGCCTGAACTGGTGAC+AGG | 0.535544 | 6.4:-16871164 | None:intergenic |
| CAGCTTTATCAAATTGGTGA+GGG | 0.538099 | 6.4:+16871317 | MS.gene42019:CDS |
| CCAGAAGATCCTGAAGTGAC+TGG | 0.538202 | 6.4:-16871185 | None:intergenic |
| CCGATCTCCGCCGATTCCAA+TGG | 0.539060 | 6.4:+16865190 | MS.gene42019:CDS |
| AACAAGAAGAAGGAGGGTCG+AGG | 0.541006 | 6.4:+16868070 | MS.gene42019:CDS |
| CTTTATCAAATTGGTGAGGG+TGG | 0.546767 | 6.4:+16871320 | MS.gene42019:CDS |
| GTGACAGGCATCATTGGTTG+TGG | 0.551259 | 6.4:-16871149 | None:intergenic |
| TCAACTGGACATGAAGTTGA+GGG | 0.551353 | 6.4:+16860058 | MS.gene42019:CDS |
| ATCAACTGGACATGAAGTTG+AGG | 0.554008 | 6.4:+16860057 | MS.gene42019:CDS |
| TCAGGGACCTTGAATTGCCT+AGG | 0.557398 | 6.4:-16859975 | None:intergenic |
| GGTGGAGAAGGAAGCATACA+TGG | 0.562243 | 6.4:-16865261 | None:intergenic |
| AGAAAGAAGGATTACAAGGA+TGG | 0.565673 | 6.4:+16860011 | MS.gene42019:CDS |
| TTCCAATAATGATTCTGCCA+TGG | 0.570860 | 6.4:+16871391 | MS.gene42019:CDS |
| CAGGGACCTTGAATTGCCTA+GGG | 0.574005 | 6.4:-16859974 | None:intergenic |
| CTTCTGCTTTCCATCAACGT+TGG | 0.575809 | 6.4:-16858934 | None:intergenic |
| GCTGAAGATTTCGACGAACA+GGG | 0.582791 | 6.4:-16871298 | None:intergenic |
| CTAGTCTGCATGCATATCCA+TGG | 0.585175 | 6.4:-16871408 | None:intergenic |
| TGAAACTCAGCCGAAGAACT+CGG | 0.586864 | 6.4:+16871253 | MS.gene42019:CDS |
| TTCTTCGCCATTGGAATCGG+CGG | 0.586954 | 6.4:-16865197 | None:intergenic |
| TCAGCTTTATCAAATTGGTG+AGG | 0.587468 | 6.4:+16871316 | MS.gene42019:CDS |
| CAAGTCTCGACGAAGTCGAT+CGG | 0.588474 | 6.4:+16865237 | MS.gene42019:CDS |
| TTGGAATCGGCGGAGATCGG+TGG | 0.591812 | 6.4:-16865187 | None:intergenic |
| TGACTGTGGTTGCCAACCCT+AGG | 0.601099 | 6.4:+16859958 | MS.gene42019:CDS |
| TGGATTTGGAAACAAGAAGA+AGG | 0.601954 | 6.4:+16868060 | MS.gene42019:CDS |
| CTGATGTTGACATGAACAAG+AGG | 0.608795 | 6.4:+16859024 | MS.gene42019:CDS |
| TTTGCTATGACTTCAATCAA+AGG | 0.610699 | 6.4:+16858963 | MS.gene42019:CDS |
| ACAAGAAGAAGGAGGGTCGA+GGG | 0.630981 | 6.4:+16868071 | MS.gene42019:CDS |
| TTTGGAAACAAGAAGAAGGA+GGG | 0.660375 | 6.4:+16868064 | MS.gene42019:CDS |
| TTCTGGCCAAACGATAGCTG+GGG | 0.665081 | 6.4:+16871202 | MS.gene42019:CDS |
| ATTTGGAAACAAGAAGAAGG+AGG | 0.667699 | 6.4:+16868063 | MS.gene42019:CDS |
| GACTTCGTCGAGACTTGACG+CGG | 0.668540 | 6.4:-16865231 | None:intergenic |
| TTCGTCGAGACTTGACGCGG+CGG | 0.696398 | 6.4:-16865228 | None:intergenic |
| CTGAGAAAGAGAATTAGCAG+CGG | 0.709453 | 6.4:-16865282 | None:intergenic |
| GTTGGAGAACATTATGACTG+TGG | 0.726380 | 6.4:+16859944 | MS.gene42019:CDS |
| CATAATGTTCTCCAACTCAG+CGG | 0.735443 | 6.4:-16859937 | None:intergenic |
| GAGTTTCACAATAATCAGAG+CGG | 0.750112 | 6.4:-16871238 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.4 | gene | 16858761 | 16871430 | 16858761 | ID=MS.gene42019 |
| chr6.4 | mRNA | 16858761 | 16871430 | 16858761 | ID=MS.gene42019.t1;Parent=MS.gene42019 |
| chr6.4 | exon | 16858761 | 16858763 | 16858761 | ID=MS.gene42019.t1.exon1;Parent=MS.gene42019.t1 |
| chr6.4 | CDS | 16858761 | 16858763 | 16858761 | ID=cds.MS.gene42019.t1;Parent=MS.gene42019.t1 |
| chr6.4 | exon | 16858885 | 16859045 | 16858885 | ID=MS.gene42019.t1.exon2;Parent=MS.gene42019.t1 |
| chr6.4 | CDS | 16858885 | 16859045 | 16858885 | ID=cds.MS.gene42019.t1;Parent=MS.gene42019.t1 |
| chr6.4 | exon | 16859920 | 16860108 | 16859920 | ID=MS.gene42019.t1.exon3;Parent=MS.gene42019.t1 |
| chr6.4 | CDS | 16859920 | 16860108 | 16859920 | ID=cds.MS.gene42019.t1;Parent=MS.gene42019.t1 |
| chr6.4 | exon | 16865154 | 16865367 | 16865154 | ID=MS.gene42019.t1.exon4;Parent=MS.gene42019.t1 |
| chr6.4 | CDS | 16865154 | 16865367 | 16865154 | ID=cds.MS.gene42019.t1;Parent=MS.gene42019.t1 |
| chr6.4 | exon | 16868045 | 16868125 | 16868045 | ID=MS.gene42019.t1.exon5;Parent=MS.gene42019.t1 |
| chr6.4 | CDS | 16868045 | 16868125 | 16868045 | ID=cds.MS.gene42019.t1;Parent=MS.gene42019.t1 |
| chr6.4 | exon | 16871083 | 16871430 | 16871083 | ID=MS.gene42019.t1.exon6;Parent=MS.gene42019.t1 |
| chr6.4 | CDS | 16871083 | 16871430 | 16871083 | ID=cds.MS.gene42019.t1;Parent=MS.gene42019.t1 |
| Gene Sequence |
| Protein sequence |
