Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene42494.t1 | XP_003620328.1 | 95.7 | 326 | 13 | 1 | 1 | 325 | 1 | 326 | 1.10E-184 | 656 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene42494.t1 | P0C042 | 54.5 | 244 | 101 | 3 | 83 | 325 | 41 | 275 | 7.7e-70 | 265.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene42494.t1 | G7KM25 | 95.7 | 326 | 13 | 1 | 1 | 325 | 1 | 326 | 7.9e-185 | 656.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene05156 | MS.gene42494 | 0.802396 | 5.72E-49 | -1.69E-46 |
| MS.gene05952 | MS.gene42494 | 0.806182 | 9.32E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene42494.t1 | MTR_6g081060 | 93.491 | 338 | 20 | 2 | 1 | 336 | 1 | 338 | 0.0 | 660 |
| MS.gene42494.t1 | MTR_0223s0010 | 50.000 | 328 | 152 | 4 | 36 | 356 | 47 | 369 | 1.26e-111 | 332 |
| MS.gene42494.t1 | MTR_1g112300 | 58.103 | 253 | 102 | 2 | 73 | 325 | 64 | 312 | 9.49e-108 | 321 |
| MS.gene42494.t1 | MTR_1g112300 | 60.515 | 233 | 91 | 1 | 93 | 325 | 1 | 232 | 1.16e-104 | 310 |
| MS.gene42494.t1 | MTR_1g044340 | 42.023 | 257 | 139 | 4 | 78 | 325 | 6 | 261 | 9.32e-63 | 204 |
| MS.gene42494.t1 | MTR_8g007405 | 40.784 | 255 | 141 | 4 | 80 | 325 | 56 | 309 | 5.41e-61 | 200 |
| MS.gene42494.t1 | MTR_8g007395 | 38.721 | 297 | 160 | 6 | 48 | 325 | 19 | 312 | 1.05e-59 | 197 |
| MS.gene42494.t1 | MTR_8g007400 | 37.152 | 323 | 185 | 6 | 51 | 356 | 23 | 344 | 1.39e-58 | 194 |
| MS.gene42494.t1 | MTR_7g029335 | 33.333 | 237 | 152 | 5 | 93 | 325 | 1 | 235 | 8.49e-39 | 140 |
| MS.gene42494.t1 | MTR_7g029330 | 33.333 | 237 | 146 | 8 | 103 | 332 | 2 | 233 | 1.12e-32 | 123 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene42494.t1 | AT1G14590 | 48.348 | 333 | 158 | 6 | 7 | 337 | 18 | 338 | 1.04e-108 | 324 |
| MS.gene42494.t1 | AT4G19975 | 49.538 | 325 | 144 | 5 | 33 | 347 | 26 | 340 | 2.00e-105 | 315 |
| MS.gene42494.t1 | AT2G02061 | 60.000 | 240 | 94 | 2 | 87 | 325 | 111 | 349 | 1.14e-104 | 315 |
| MS.gene42494.t1 | AT2G02061 | 60.000 | 240 | 94 | 2 | 87 | 325 | 111 | 349 | 1.49e-104 | 314 |
| MS.gene42494.t1 | AT5G44820 | 47.059 | 340 | 160 | 6 | 23 | 347 | 17 | 351 | 3.38e-102 | 307 |
| MS.gene42494.t1 | AT4G19970 | 43.750 | 304 | 158 | 5 | 34 | 325 | 41 | 343 | 2.68e-88 | 271 |
| MS.gene42494.t1 | AT4G15970 | 54.918 | 244 | 100 | 3 | 83 | 325 | 32 | 266 | 1.80e-87 | 269 |
| MS.gene42494.t1 | AT1G28695 | 38.403 | 263 | 151 | 5 | 69 | 325 | 30 | 287 | 3.15e-53 | 179 |
| MS.gene42494.t1 | AT1G28700 | 38.346 | 266 | 152 | 5 | 67 | 325 | 36 | 296 | 7.79e-53 | 179 |
| MS.gene42494.t1 | AT1G28710 | 37.407 | 270 | 156 | 6 | 59 | 319 | 27 | 292 | 2.46e-51 | 175 |
| MS.gene42494.t1 | AT1G28710 | 37.407 | 270 | 156 | 6 | 59 | 319 | 27 | 292 | 2.46e-51 | 175 |
| MS.gene42494.t1 | AT1G28710 | 37.407 | 270 | 156 | 6 | 59 | 319 | 27 | 292 | 2.46e-51 | 175 |
| MS.gene42494.t1 | AT5G40900 | 32.000 | 275 | 171 | 5 | 84 | 351 | 57 | 322 | 9.77e-41 | 147 |
Find 61 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAATTTGGTTCTACAAATTT+TGG | 0.142477 | 6.1:+67885366 | MS.gene42494:CDS |
| AAGCTTCTTATTTGTAATTA+AGG | 0.172101 | 6.1:-67885456 | None:intergenic |
| TGAAATAATCACAAGCAATT+TGG | 0.182799 | 6.1:-67885274 | None:intergenic |
| TTCTTAAGCACAAGCTATTT+TGG | 0.185065 | 6.1:+67885488 | MS.gene42494:CDS |
| AACAATTCTGCTTCTCCTTT+TGG | 0.199551 | 6.1:+67878833 | MS.gene42494:CDS |
| TCTTGGTGCTGTTCTTCATT+TGG | 0.209687 | 6.1:+67884336 | MS.gene42494:CDS |
| CTTGGTGCTGTTCTTCATTT+GGG | 0.259335 | 6.1:+67884337 | MS.gene42494:CDS |
| TTGTAGAACCAAATTGTTCT+TGG | 0.269685 | 6.1:-67885359 | None:intergenic |
| TGTCAATCTAGCAAGGATTT+TGG | 0.287241 | 6.1:+67885518 | MS.gene42494:CDS |
| AATCAAATCCAAGAACAATT+TGG | 0.309827 | 6.1:+67885351 | MS.gene42494:CDS |
| AGCTTCTTATTTGTAATTAA+GGG | 0.327364 | 6.1:-67885455 | None:intergenic |
| ATTGCTTGTGATTATTTCAA+TGG | 0.335617 | 6.1:+67885278 | MS.gene42494:CDS |
| TGGAGAAGAATTGAATTTCT+TGG | 0.355189 | 6.1:+67884319 | MS.gene42494:CDS |
| CAAATAAGAAGCTTAAGATT+AGG | 0.357408 | 6.1:+67885465 | MS.gene42494:CDS |
| TTAAGAACACTCTTTAGCTT+TGG | 0.370284 | 6.1:-67884022 | None:intergenic |
| GATCCAATAGCATTTGTGTT+TGG | 0.405430 | 6.1:-67884156 | None:intergenic |
| TGCTAATTGTTGTGTTGGCT+TGG | 0.407831 | 6.1:+67885559 | MS.gene42494:CDS |
| ACATATGTGAATCCTCCATT+TGG | 0.413713 | 6.1:-67885326 | None:intergenic |
| GAACCAAACACAAATGCTAT+TGG | 0.415826 | 6.1:+67884153 | MS.gene42494:CDS |
| AACAAGTCATGGAAGTCAAT+TGG | 0.426656 | 6.1:+67878725 | MS.gene42494:CDS |
| TCTTTGTAAAATTGTTTGAA+TGG | 0.440475 | 6.1:-67885242 | None:intergenic |
| CCATGACTTGTTCCCTTCTC+CGG | 0.447086 | 6.1:-67878714 | None:intergenic |
| TCATGGAAGTCAATTGGAAC+AGG | 0.450424 | 6.1:+67878731 | MS.gene42494:CDS |
| TGTTACCAACTTCAAACTAA+AGG | 0.466478 | 6.1:+67884241 | MS.gene42494:CDS |
| ACATCTTGATCATGCATAGT+TGG | 0.467730 | 6.1:-67885410 | None:intergenic |
| TGTCACCTTTAGTTTGAAGT+TGG | 0.471511 | 6.1:-67884246 | None:intergenic |
| AGTATCTCACATTACTTCAA+TGG | 0.493300 | 6.1:+67878866 | MS.gene42494:CDS |
| CACATCATGTGTAAATATGT+TGG | 0.494038 | 6.1:-67884298 | None:intergenic |
| ATACTAGCAGGAAGACCAAA+AGG | 0.495282 | 6.1:-67878848 | None:intergenic |
| GTCGACAACGTCGCCGGAGA+AGG | 0.501945 | 6.1:+67878701 | MS.gene42494:CDS |
| GACGGAATCATCGTCTTCTC+CGG | 0.516029 | 6.1:+67893414 | MS.gene42494:CDS |
| TGGATAGTTGTGCATGTCAA+AGG | 0.516348 | 6.1:-67885306 | None:intergenic |
| CCGGAGAAGGGAACAAGTCA+TGG | 0.519589 | 6.1:+67878714 | MS.gene42494:CDS |
| ATGCATGCTAATTGTTGTGT+TGG | 0.526017 | 6.1:+67885554 | MS.gene42494:CDS |
| GACATGCACAACTATCCAAA+TGG | 0.526709 | 6.1:+67885311 | MS.gene42494:CDS |
| TCTTAAACAAGCATCAATGA+AGG | 0.526752 | 6.1:+67884039 | MS.gene42494:CDS |
| AAGTAATGTGAGATACTAGC+AGG | 0.540167 | 6.1:-67878860 | None:intergenic |
| AATTGATCAAATATTGAACC+TGG | 0.540784 | 6.1:-67884106 | None:intergenic |
| TTCTCCGGCGACGTTGTCGA+CGG | 0.543832 | 6.1:-67878699 | None:intergenic |
| AAGAATCATTATGCTGCTGC+TGG | 0.552708 | 6.1:+67878674 | MS.gene42494:CDS |
| CCAAAACGAAAGCAAAATGT+AGG | 0.553786 | 6.1:+67893546 | MS.gene42494:CDS |
| TCACAACATTGAATGATGCA+TGG | 0.566676 | 6.1:+67884077 | MS.gene42494:CDS |
| GGTGCCGTCGACAACGTCGC+CGG | 0.568256 | 6.1:+67878695 | MS.gene42494:CDS |
| GTCTTCTCCGGTGAGAAAGA+CGG | 0.568972 | 6.1:+67893426 | MS.gene42494:CDS |
| TCGACAACGTCGCCGGAGAA+GGG | 0.575557 | 6.1:+67878702 | MS.gene42494:CDS |
| ATCATCTTGTGGTAATAACA+TGG | 0.577012 | 6.1:+67884176 | MS.gene42494:CDS |
| CAACATATTTACACATGATG+TGG | 0.580286 | 6.1:+67884299 | MS.gene42494:CDS |
| ACGGAATCCGTCTTTCTCAC+CGG | 0.588162 | 6.1:-67893433 | None:intergenic |
| CACAACATTGAATGATGCAT+GGG | 0.590967 | 6.1:+67884078 | MS.gene42494:CDS |
| TCTTATCTTCTTCGAAACTG+AGG | 0.598561 | 6.1:+67893466 | MS.gene42494:CDS |
| CTTAAACAAGCATCAATGAA+GGG | 0.599814 | 6.1:+67884040 | MS.gene42494:CDS |
| AAGATAAGAACAAGAACGGA+CGG | 0.602343 | 6.1:-67893452 | None:intergenic |
| AATGATGCATGGGCTGAACC+AGG | 0.607742 | 6.1:+67884088 | MS.gene42494:CDS |
| AATGCTATTGGATCATCTTG+TGG | 0.609437 | 6.1:+67884165 | MS.gene42494:CDS |
| ACTGTCGCTTTCTAGAACGA+CGG | 0.611614 | 6.1:+67893396 | MS.gene42494:intron |
| GAAGAAGATAAGAACAAGAA+CGG | 0.614285 | 6.1:-67893456 | None:intergenic |
| TTGTGTTGGCTTGGAGAACA+AGG | 0.616497 | 6.1:+67885568 | MS.gene42494:CDS |
| TGCAGGACACTGATATAATG+TGG | 0.629906 | 6.1:+67885210 | MS.gene42494:intron |
| TCATCTTGTGGTAATAACAT+GGG | 0.641239 | 6.1:+67884177 | MS.gene42494:CDS |
| ATGCACAACTATCCAAATGG+AGG | 0.677945 | 6.1:+67885314 | MS.gene42494:CDS |
| GTAGGAGAAAACACAGACGA+AGG | 0.693651 | 6.1:+67893564 | MS.gene42494:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.1 | gene | 67878671 | 67895153 | 67878671 | ID=MS.gene42494 |
| chr6.1 | mRNA | 67878671 | 67895153 | 67878671 | ID=MS.gene42494.t1;Parent=MS.gene42494 |
| chr6.1 | exon | 67878671 | 67878901 | 67878671 | ID=MS.gene42494.t1.exon1;Parent=MS.gene42494.t1 |
| chr6.1 | CDS | 67878671 | 67878901 | 67878671 | ID=cds.MS.gene42494.t1;Parent=MS.gene42494.t1 |
| chr6.1 | exon | 67884010 | 67884378 | 67884010 | ID=MS.gene42494.t1.exon2;Parent=MS.gene42494.t1 |
| chr6.1 | CDS | 67884010 | 67884378 | 67884010 | ID=cds.MS.gene42494.t1;Parent=MS.gene42494.t1 |
| chr6.1 | exon | 67885215 | 67885589 | 67885215 | ID=MS.gene42494.t1.exon3;Parent=MS.gene42494.t1 |
| chr6.1 | CDS | 67885215 | 67885589 | 67885215 | ID=cds.MS.gene42494.t1;Parent=MS.gene42494.t1 |
| chr6.1 | exon | 67893411 | 67893585 | 67893411 | ID=MS.gene42494.t1.exon4;Parent=MS.gene42494.t1 |
| chr6.1 | CDS | 67893411 | 67893585 | 67893411 | ID=cds.MS.gene42494.t1;Parent=MS.gene42494.t1 |
| chr6.1 | exon | 67895146 | 67895153 | 67895146 | ID=MS.gene42494.t1.exon5;Parent=MS.gene42494.t1 |
| chr6.1 | CDS | 67895146 | 67895153 | 67895146 | ID=cds.MS.gene42494.t1;Parent=MS.gene42494.t1 |
| Gene Sequence |
| Protein sequence |
