Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene05156.t1 | XP_003620328.1 | 94.4 | 375 | 20 | 1 | 1 | 374 | 1 | 375 | 2.00E-209 | 738.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene05156.t1 | P0C042 | 50.5 | 279 | 127 | 4 | 83 | 360 | 41 | 309 | 1.2e-74 | 282.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene05156.t1 | G7KM25 | 94.4 | 375 | 20 | 1 | 1 | 374 | 1 | 375 | 1.4e-209 | 738.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene05156 | MS.gene052053 | 0.816956 | 4.23E-52 | -1.69E-46 |
| MS.gene05156 | MS.gene052560 | 0.816584 | 5.13E-52 | -1.69E-46 |
| MS.gene05156 | MS.gene052579 | 0.813574 | 2.39E-51 | -1.69E-46 |
| MS.gene05156 | MS.gene056185 | 0.815884 | 7.35E-52 | -1.69E-46 |
| MS.gene05156 | MS.gene065380 | 0.804508 | 2.09E-49 | -1.69E-46 |
| MS.gene05156 | MS.gene066723 | 0.80526 | 1.45E-49 | -1.69E-46 |
| MS.gene05156 | MS.gene067531 | 0.811575 | 6.54E-51 | -1.69E-46 |
| MS.gene05156 | MS.gene072436 | 0.837239 | 5.84E-57 | -1.69E-46 |
| MS.gene05156 | MS.gene073500 | 0.800945 | 1.14E-48 | -1.69E-46 |
| MS.gene05156 | MS.gene074124 | 0.814889 | 1.22E-51 | -1.69E-46 |
| MS.gene05156 | MS.gene20344 | 0.815911 | 7.25E-52 | -1.69E-46 |
| MS.gene05156 | MS.gene24408 | 0.811015 | 8.65E-51 | -1.69E-46 |
| MS.gene05156 | MS.gene29303 | 0.800412 | 1.46E-48 | -1.69E-46 |
| MS.gene05156 | MS.gene32208 | 0.803842 | 2.87E-49 | -1.69E-46 |
| MS.gene05156 | MS.gene33837 | 0.81185 | 5.70E-51 | -1.69E-46 |
| MS.gene05156 | MS.gene40433 | 0.823225 | 1.55E-53 | -1.69E-46 |
| MS.gene05156 | MS.gene42494 | 0.802396 | 5.72E-49 | -1.69E-46 |
| MS.gene05156 | MS.gene46055 | 0.810321 | 1.22E-50 | -1.69E-46 |
| MS.gene05156 | MS.gene46237 | 0.808793 | 2.60E-50 | -1.69E-46 |
| MS.gene05156 | MS.gene49911 | 0.819647 | 1.04E-52 | -1.69E-46 |
| MS.gene05156 | MS.gene54952 | 0.804723 | 1.88E-49 | -1.69E-46 |
| MS.gene05156 | MS.gene56769 | 0.823428 | 1.39E-53 | -1.69E-46 |
| MS.gene05156 | MS.gene65297 | 0.827101 | 1.88E-54 | -1.69E-46 |
| MS.gene05156 | MS.gene65364 | 0.826062 | 3.33E-54 | -1.69E-46 |
| MS.gene05156 | MS.gene65365 | 0.818365 | 2.04E-52 | -1.69E-46 |
| MS.gene05156 | MS.gene67131 | 0.818691 | 1.72E-52 | -1.69E-46 |
| MS.gene05156 | MS.gene73644 | 0.817051 | 4.03E-52 | -1.69E-46 |
| MS.gene05156 | MS.gene80055 | 0.832922 | 7.17E-56 | -1.69E-46 |
| MS.gene05156 | MS.gene85401 | 0.805249 | 1.46E-49 | -1.69E-46 |
| MS.gene05156 | MS.gene89256 | 0.80285 | 4.61E-49 | -1.69E-46 |
| MS.gene05156 | MS.gene94266 | 0.817796 | 2.74E-52 | -1.69E-46 |
| MS.gene05156 | MS.gene98636 | 0.833287 | 5.81E-56 | -1.69E-46 |
| MS.gene05156 | MS.gene99191 | 0.811897 | 5.56E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene05156.t1 | MTR_6g081060 | 94.400 | 375 | 20 | 1 | 1 | 374 | 1 | 375 | 0.0 | 740 |
| MS.gene05156.t1 | MTR_0223s0010 | 51.497 | 334 | 153 | 3 | 36 | 362 | 47 | 378 | 1.54e-124 | 367 |
| MS.gene05156.t1 | MTR_1g112300 | 56.164 | 292 | 124 | 2 | 73 | 364 | 64 | 351 | 8.13e-123 | 362 |
| MS.gene05156.t1 | MTR_1g112300 | 58.088 | 272 | 113 | 1 | 93 | 364 | 1 | 271 | 1.25e-119 | 351 |
| MS.gene05156.t1 | MTR_1g044340 | 42.545 | 275 | 148 | 4 | 78 | 343 | 6 | 279 | 8.51e-69 | 221 |
| MS.gene05156.t1 | MTR_8g007405 | 39.655 | 290 | 163 | 5 | 80 | 360 | 56 | 342 | 1.19e-66 | 217 |
| MS.gene05156.t1 | MTR_8g007395 | 38.012 | 342 | 182 | 8 | 48 | 362 | 19 | 357 | 1.51e-66 | 217 |
| MS.gene05156.t1 | MTR_8g007400 | 38.532 | 327 | 183 | 6 | 51 | 360 | 23 | 348 | 3.15e-66 | 216 |
| MS.gene05156.t1 | MTR_7g029335 | 33.088 | 272 | 176 | 5 | 93 | 360 | 1 | 270 | 1.40e-46 | 162 |
| MS.gene05156.t1 | MTR_7g029330 | 31.818 | 264 | 169 | 7 | 103 | 360 | 2 | 260 | 9.03e-39 | 141 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene05156.t1 | AT1G14590 | 46.883 | 369 | 183 | 5 | 7 | 374 | 18 | 374 | 9.33e-123 | 363 |
| MS.gene05156.t1 | AT2G02061 | 56.631 | 279 | 119 | 2 | 87 | 364 | 111 | 388 | 1.38e-117 | 350 |
| MS.gene05156.t1 | AT2G02061 | 57.091 | 275 | 116 | 2 | 87 | 360 | 111 | 384 | 1.07e-116 | 347 |
| MS.gene05156.t1 | AT4G19975 | 49.408 | 338 | 153 | 4 | 33 | 361 | 26 | 354 | 2.07e-115 | 343 |
| MS.gene05156.t1 | AT5G44820 | 54.704 | 287 | 124 | 3 | 76 | 360 | 82 | 364 | 2.26e-112 | 335 |
| MS.gene05156.t1 | AT4G19970 | 43.363 | 339 | 176 | 6 | 34 | 360 | 41 | 375 | 1.65e-97 | 298 |
| MS.gene05156.t1 | AT4G15970 | 51.064 | 282 | 121 | 5 | 83 | 360 | 32 | 300 | 3.28e-92 | 283 |
| MS.gene05156.t1 | AT1G28700 | 38.014 | 292 | 169 | 5 | 67 | 351 | 36 | 322 | 2.90e-59 | 197 |
| MS.gene05156.t1 | AT1G28695 | 38.284 | 303 | 166 | 7 | 69 | 363 | 30 | 319 | 9.46e-59 | 196 |
| MS.gene05156.t1 | AT1G28710 | 36.196 | 326 | 183 | 9 | 59 | 371 | 27 | 340 | 1.27e-57 | 193 |
| MS.gene05156.t1 | AT1G28710 | 36.196 | 326 | 183 | 9 | 59 | 371 | 27 | 340 | 1.27e-57 | 193 |
| MS.gene05156.t1 | AT1G28710 | 36.196 | 326 | 183 | 9 | 59 | 371 | 27 | 340 | 1.27e-57 | 193 |
| MS.gene05156.t1 | AT5G40900 | 34.520 | 281 | 157 | 6 | 84 | 357 | 57 | 317 | 5.49e-45 | 159 |
Find 63 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAATTTGGTTCTACAAATTT+TGG | 0.142477 | 6.4:+51552133 | MS.gene05156:CDS |
| AAGCTTCTTATTTGTAATTA+AGG | 0.172101 | 6.4:-51552223 | None:intergenic |
| TTCTTAAGCACAAGCTATTT+TGG | 0.185065 | 6.4:+51552255 | MS.gene05156:CDS |
| AACAATTCTGCTTCTCCTTT+TGG | 0.199551 | 6.4:+51544828 | MS.gene05156:CDS |
| TCTTGGTGCTGTTCTTCATT+TGG | 0.209687 | 6.4:+51551109 | MS.gene05156:CDS |
| TGTCAATCTAGCAAGGATTT+TGG | 0.246249 | 6.4:+51552285 | MS.gene05156:CDS |
| CTTGGTGCTGTTCTTCATTT+GGG | 0.259335 | 6.4:+51551110 | MS.gene05156:CDS |
| TCGAATATGCAAGAGGAAAA+AGG | 0.267045 | 6.4:-51555840 | None:intergenic |
| TTGTAGAACCAAATTGTTCT+TGG | 0.269685 | 6.4:-51552126 | None:intergenic |
| ATTACTTCCTTTAGGATAAC+TGG | 0.274252 | 6.4:+51555867 | MS.gene05156:CDS |
| AATCAAATCCAAGAACAATT+TGG | 0.309827 | 6.4:+51552118 | MS.gene05156:CDS |
| GCCATTACAGCCATTTCATT+TGG | 0.320039 | 6.4:-51562058 | None:intergenic |
| AGCTTCTTATTTGTAATTAA+GGG | 0.327364 | 6.4:-51552222 | None:intergenic |
| ATTGCTTGTGATTATTTCAA+TGG | 0.335617 | 6.4:+51552045 | MS.gene05156:CDS |
| TCGATATTATTACTTCCTTT+AGG | 0.336754 | 6.4:+51555859 | MS.gene05156:CDS |
| TTATGTCTTGGTCACACTTA+AGG | 0.344852 | 6.4:+51555953 | MS.gene05156:CDS |
| GCAGAACACGACACGTGTTC+TGG | 0.345325 | 6.4:+51562079 | MS.gene05156:CDS |
| TGGAGAAGAATTGAATTTCT+TGG | 0.355189 | 6.4:+51551092 | MS.gene05156:CDS |
| CAAATAAGAAGCTTAAGATT+AGG | 0.357408 | 6.4:+51552232 | MS.gene05156:CDS |
| TTAAGAACACTCTTTAGCTT+TGG | 0.370284 | 6.4:-51550795 | None:intergenic |
| CCATGACTTGTTCCCTTCTC+CGG | 0.397106 | 6.4:-51544709 | None:intergenic |
| GATCCAATAGCATTTGTGTT+TGG | 0.405430 | 6.4:-51550929 | None:intergenic |
| TGCTAATTGTTGTGTTGGCT+TGG | 0.407831 | 6.4:+51552326 | MS.gene05156:CDS |
| ACATATGTGAATCCTCCATT+TGG | 0.413713 | 6.4:-51552093 | None:intergenic |
| GAACCAAACACAAATGCTAT+TGG | 0.415826 | 6.4:+51550926 | MS.gene05156:CDS |
| GAACTTCAATCTTTATGTCT+TGG | 0.420107 | 6.4:+51555941 | MS.gene05156:CDS |
| AACAAGTCATGGAAGTCAAT+TGG | 0.426656 | 6.4:+51544720 | MS.gene05156:CDS |
| TGAGGATTGGAAGAAGTATA+TGG | 0.432395 | 6.4:+51552380 | MS.gene05156:CDS |
| TTCATTACAGCCAAATGAAA+TGG | 0.433685 | 6.4:+51562048 | MS.gene05156:CDS |
| TCTTTGTAAAATTGTTTGAA+TGG | 0.440475 | 6.4:-51552009 | None:intergenic |
| AAGTAATGTGAGATAGTAGC+AGG | 0.464851 | 6.4:-51544855 | None:intergenic |
| TGTTACCAACTTCAAACTAA+AGG | 0.466478 | 6.4:+51551014 | MS.gene05156:CDS |
| ACATCTTGATCATGCATAGT+TGG | 0.467730 | 6.4:-51552177 | None:intergenic |
| TGTCACCTTTAGTTTGAAGT+TGG | 0.471511 | 6.4:-51551019 | None:intergenic |
| ATAGTAGCAGGAAGACCAAA+AGG | 0.473505 | 6.4:-51544843 | None:intergenic |
| ACTATCTCACATTACTTCAA+TGG | 0.475598 | 6.4:+51544861 | MS.gene05156:CDS |
| GCCAAATGAAATGGCTGTAA+TGG | 0.484797 | 6.4:+51562057 | MS.gene05156:CDS |
| TCATGGAAGTCAATTGGAAC+AGG | 0.485736 | 6.4:+51544726 | MS.gene05156:CDS |
| CACATCATGTGTAAATATGT+TGG | 0.494038 | 6.4:-51551071 | None:intergenic |
| TGGATAGTTGTGCATGTCAA+AGG | 0.516348 | 6.4:-51552073 | None:intergenic |
| GTCGACAACGTCACCGGAGA+AGG | 0.520774 | 6.4:+51544696 | MS.gene05156:CDS |
| CCGGAGAAGGGAACAAGTCA+TGG | 0.521051 | 6.4:+51544709 | MS.gene05156:CDS |
| ATGCATGCTAATTGTTGTGT+TGG | 0.526017 | 6.4:+51552321 | MS.gene05156:CDS |
| GACATGCACAACTATCCAAA+TGG | 0.526709 | 6.4:+51552078 | MS.gene05156:CDS |
| TCTTAAACAAGCATCAATGA+AGG | 0.526752 | 6.4:+51550812 | MS.gene05156:CDS |
| TCTAAAACCAGTTATCCTAA+AGG | 0.533019 | 6.4:-51555874 | None:intergenic |
| AATTGATCAAATATTGAACC+TGG | 0.540784 | 6.4:-51550879 | None:intergenic |
| AAGAATCATTATGCTGCTGC+TGG | 0.552708 | 6.4:+51544669 | MS.gene05156:CDS |
| TCACAACATTGAATGATGCA+TGG | 0.566676 | 6.4:+51550850 | MS.gene05156:CDS |
| TTCTCCGGTGACGTTGTCGA+CGG | 0.570637 | 6.4:-51544694 | None:intergenic |
| GGTGCCGTCGACAACGTCAC+CGG | 0.570753 | 6.4:+51544690 | MS.gene05156:CDS |
| AATAATATCGAATATGCAAG+AGG | 0.572151 | 6.4:-51555847 | None:intergenic |
| ATCATCTTGTGGTAATAACA+TGG | 0.577012 | 6.4:+51550949 | MS.gene05156:CDS |
| CAACATATTTACACATGATG+TGG | 0.580286 | 6.4:+51551072 | MS.gene05156:CDS |
| TCGACAACGTCACCGGAGAA+GGG | 0.587959 | 6.4:+51544697 | MS.gene05156:CDS |
| CACAACATTGAATGATGCAT+GGG | 0.590967 | 6.4:+51550851 | MS.gene05156:CDS |
| CTTAAACAAGCATCAATGAA+GGG | 0.599814 | 6.4:+51550813 | MS.gene05156:CDS |
| AATGATGCATGGGCTGAACC+AGG | 0.607742 | 6.4:+51550861 | MS.gene05156:CDS |
| AATGCTATTGGATCATCTTG+TGG | 0.609437 | 6.4:+51550938 | MS.gene05156:CDS |
| TTGTGTTGGCTTGGAGAACA+AGG | 0.616497 | 6.4:+51552335 | MS.gene05156:CDS |
| TGCAGGACACTGATATAATG+TGG | 0.629906 | 6.4:+51551977 | MS.gene05156:intron |
| TCATCTTGTGGTAATAACAT+GGG | 0.641239 | 6.4:+51550950 | MS.gene05156:CDS |
| ATGCACAACTATCCAAATGG+AGG | 0.677945 | 6.4:+51552081 | MS.gene05156:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.4 | gene | 51544666 | 51562153 | 51544666 | ID=MS.gene05156 |
| chr6.4 | mRNA | 51544666 | 51562153 | 51544666 | ID=MS.gene05156.t1;Parent=MS.gene05156 |
| chr6.4 | exon | 51544666 | 51544896 | 51544666 | ID=MS.gene05156.t1.exon1;Parent=MS.gene05156.t1 |
| chr6.4 | CDS | 51544666 | 51544896 | 51544666 | ID=cds.MS.gene05156.t1;Parent=MS.gene05156.t1 |
| chr6.4 | exon | 51550783 | 51551151 | 51550783 | ID=MS.gene05156.t1.exon2;Parent=MS.gene05156.t1 |
| chr6.4 | CDS | 51550783 | 51551151 | 51550783 | ID=cds.MS.gene05156.t1;Parent=MS.gene05156.t1 |
| chr6.4 | exon | 51551982 | 51552463 | 51551982 | ID=MS.gene05156.t1.exon3;Parent=MS.gene05156.t1 |
| chr6.4 | CDS | 51551982 | 51552463 | 51551982 | ID=cds.MS.gene05156.t1;Parent=MS.gene05156.t1 |
| chr6.4 | exon | 51555794 | 51555974 | 51555794 | ID=MS.gene05156.t1.exon4;Parent=MS.gene05156.t1 |
| chr6.4 | CDS | 51555794 | 51555974 | 51555794 | ID=cds.MS.gene05156.t1;Parent=MS.gene05156.t1 |
| chr6.4 | exon | 51562046 | 51562153 | 51562046 | ID=MS.gene05156.t1.exon5;Parent=MS.gene05156.t1 |
| chr6.4 | CDS | 51562046 | 51562153 | 51562046 | ID=cds.MS.gene05156.t1;Parent=MS.gene05156.t1 |
| Gene Sequence |
| Protein sequence |
