AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene42496

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene42496.t1 MTR_6g478000 83.628 678 20 4 132 788 234 841 0.0 1137
MS.gene42496.t1 MTR_6g478000 100.000 52 0 0 83 134 81 132 1.46e-26 116
MS.gene42496.t1 MTR_6g478030 93.143 525 16 1 132 636 996 1520 0.0 1014
MS.gene42496.t1 MTR_5g076830 75.294 595 126 2 135 709 235 828 0.0 964
MS.gene42496.t1 MTR_5g076830 56.250 64 27 1 83 145 79 142 2.32e-16 84.0
MS.gene42496.t1 MTR_3g064610 73.077 598 140 2 132 708 235 832 0.0 937
MS.gene42496.t1 MTR_3g064610 57.692 52 22 0 83 134 82 133 1.17e-13 75.5
MS.gene42496.t1 MTR_4g124660 59.076 606 219 3 108 687 200 802 0.0 761
MS.gene42496.t1 MTR_2g044070 60.000 575 211 1 132 687 226 800 0.0 759
MS.gene42496.t1 MTR_7g108930 61.821 571 198 1 139 689 235 805 0.0 758
MS.gene42496.t1 MTR_1g088170 61.101 563 199 1 139 681 238 800 0.0 741
MS.gene42496.t1 MTR_4g124660 60.759 553 188 3 108 634 200 749 0.0 704
MS.gene42496.t1 MTR_6g478020 93.651 252 11 2 516 762 1 252 7.65e-160 497
MS.gene42496.t1 MTR_0277s0020 81.111 270 14 3 524 788 1159 1396 8.08e-135 434
MS.gene42496.t1 MTR_0277s0030 88.679 159 13 2 635 788 65 223 2.36e-90 283
MS.gene42496.t1 MTR_0277s0030 55.882 68 10 1 480 527 1 68 8.57e-13 68.9
MS.gene42496.t1 MTR_0419s0050 67.626 139 5 2 480 618 1 99 4.64e-54 182
MS.gene42496.t1 MTR_3g047380 25.490 510 307 16 183 638 167 657 4.49e-33 137
MS.gene42496.t1 MTR_4g115620 25.477 577 323 23 163 657 125 676 7.16e-33 137
MS.gene42496.t1 MTR_0419s0010 96.552 58 2 0 624 681 12 69 9.76e-33 121
MS.gene42496.t1 MTR_5g091340 25.813 523 303 20 183 635 171 678 1.15e-31 133
MS.gene42496.t1 MTR_0419s0020 90.909 66 6 0 692 757 17 82 1.19e-31 119
MS.gene42496.t1 MTR_6g081130 64.815 108 25 3 689 788 14 116 3.85e-31 118
MS.gene42496.t1 MTR_0419s0060 80.000 55 7 1 689 739 14 68 7.05e-19 81.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene42496.t1 AT1G73370 72.727 583 137 2 135 697 237 817 0.0 909
MS.gene42496.t1 AT1G73370 65.455 55 19 0 83 137 81 135 1.41e-16 85.1
MS.gene42496.t1 AT1G73370 72.727 583 137 2 135 697 237 817 0.0 909
MS.gene42496.t1 AT1G73370 65.455 55 19 0 83 137 81 135 1.41e-16 85.1
MS.gene42496.t1 AT1G73370 72.727 583 137 2 135 697 193 773 0.0 907
MS.gene42496.t1 AT1G73370 65.455 55 19 0 83 137 37 91 1.64e-16 84.7
MS.gene42496.t1 AT5G37180 72.775 573 136 1 135 687 39 611 0.0 896
MS.gene42496.t1 AT5G37180 72.775 573 136 1 135 687 227 799 0.0 896
MS.gene42496.t1 AT5G37180 53.846 52 24 0 83 134 74 125 7.64e-12 69.7
MS.gene42496.t1 AT5G37180 72.694 553 131 1 155 687 1 553 0.0 863
MS.gene42496.t1 AT4G02280 62.500 568 193 1 139 686 237 804 0.0 771
MS.gene42496.t1 AT3G43190 61.739 575 201 1 132 687 230 804 0.0 765
MS.gene42496.t1 AT3G43190 61.739 575 201 1 132 687 230 804 0.0 765
MS.gene42496.t1 AT5G20830 60.485 577 209 1 132 689 230 806 0.0 763
MS.gene42496.t1 AT5G20830 60.485 577 209 1 132 689 230 806 0.0 763
MS.gene42496.t1 AT5G20830 60.485 577 209 1 132 689 230 806 0.0 763
MS.gene42496.t1 AT5G49190 60.034 583 210 2 125 687 223 802 0.0 755
MS.gene42496.t1 AT5G49190 60.034 583 210 2 125 687 213 792 0.0 755
MS.gene42496.t1 AT5G49190 60.034 583 210 2 125 687 157 736 0.0 754
MS.gene42496.t1 AT4G10120 25.388 516 312 17 183 647 23 516 2.42e-32 135
MS.gene42496.t1 AT4G10120 25.388 516 312 17 183 647 197 690 5.89e-32 134
MS.gene42496.t1 AT4G10120 25.388 516 312 17 183 647 197 690 5.89e-32 134
MS.gene42496.t1 AT4G10120 25.388 516 312 17 183 647 197 690 5.89e-32 134
MS.gene42496.t1 AT1G04920 26.443 537 311 21 179 645 168 690 9.45e-32 134
MS.gene42496.t1 AT5G11110 25.187 536 317 20 183 662 177 684 1.59e-31 133
MS.gene42496.t1 AT4G10120 24.848 495 301 15 202 647 17 489 8.87e-31 130
MS.gene42496.t1 AT5G20280 25.182 548 303 21 179 656 166 676 9.84e-31 130

Find 174 sgRNAs with CRISPR-Local

Find 0 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCATTATCTCTCAAAGTATT+TGG 0.129152 6.1:+67781038 MS.gene42496:CDS
TTGAATATGGGATCAATTTA+TGG 0.132045 6.1:+67779677 MS.gene42496:CDS
AAAAGTGATTCCAATTTAAT+TGG 0.164315 6.1:-67776375 None:intergenic
CAAAGTGGTGATCTTTATTC+TGG 0.182888 6.1:+67780973 MS.gene42496:CDS
TTAGGCCTAATCCTGGTTTC+TGG 0.186079 6.1:+67774860 MS.gene42496:CDS
TGGGATCAATTTATGGGTTT+TGG 0.205721 6.1:+67779684 MS.gene42496:CDS
CCTTGCCATTGAGAAGATTA+TGG 0.216432 6.1:-67778893 None:intergenic
ACCAAGTTTAGAAGCTATTA+AGG 0.217800 6.1:-67777695 None:intergenic
TGTTAGCTTAGATTCAATTT+TGG 0.228497 6.1:-67780266 None:intergenic
TGCAAACAAAGTGTTGAATA+TGG 0.228861 6.1:+67779664 MS.gene42496:CDS
TTCAATCTCAGTACACAAAT+TGG 0.234890 6.1:+67772722 MS.gene42496:intron
TACCAAGATTCTTGAACTTA+TGG 0.238387 6.1:+67777618 MS.gene42496:CDS
TTGACATTGGTTGAGGTTCT+TGG 0.240050 6.1:-67780147 None:intergenic
ACATTCTTAGAGTCCCTTTC+TGG 0.252632 6.1:+67777404 MS.gene42496:CDS
AGCAGGAGATCCAATTAAAT+TGG 0.270611 6.1:+67776365 MS.gene42496:CDS
GGAAAACCCGACCTCATAAT+TGG 0.277305 6.1:+67777643 MS.gene42496:CDS
GGCCTTGCAACTCAATCTTT+TGG 0.279108 6.1:-67777080 None:intergenic
TGAGCAAATCTTTCTAAATA+AGG 0.291781 6.1:-67777475 None:intergenic
TTTGGTCAAGCAGATGTTCT+AGG 0.295889 6.1:+67776450 MS.gene42496:CDS
ATGAAGATTCAGATGCTAAA+TGG 0.297761 6.1:+67778096 MS.gene42496:CDS
CCAGCATTGTATGAAGCTTT+TGG 0.302612 6.1:+67779189 MS.gene42496:CDS
TCAACCTTCCAGCAATTTCT+TGG 0.309492 6.1:-67778211 None:intergenic
GGATTACGTTGGTTGCTTCC+AGG 0.317665 6.1:+67780994 MS.gene42496:CDS
GAAACTATGTTCCAATGTTT+AGG 0.318753 6.1:-67779381 None:intergenic
TCTGCTTGGCTCTTTCTATA+AGG 0.323203 6.1:-67772748 None:intergenic
TTTCATATTGATCCTCATAA+TGG 0.327462 6.1:+67779306 MS.gene42496:CDS
GAATCTGAGGCTTCACATTA+AGG 0.333564 6.1:-67777101 None:intergenic
TGAGGTTCTTGGGGTACCTT+AGG 0.341726 6.1:-67780136 None:intergenic
TTCTCTATTCATGGATACTT+TGG 0.345725 6.1:+67776432 MS.gene42496:CDS
TAGAGAATGGGGATTTGATA+AGG 0.349353 6.1:+67776281 MS.gene42496:CDS
TGAATATGGGATCAATTTAT+GGG 0.351676 6.1:+67779678 MS.gene42496:CDS
ATCCATAAGTTCAAGAATCT+TGG 0.355620 6.1:-67777620 None:intergenic
CTTGCCATTGAGAAGATTAT+GGG 0.361356 6.1:-67778892 None:intergenic
TGACATTGGTTGAGGTTCTT+GGG 0.367456 6.1:-67780146 None:intergenic
ATAACAATGTTGAACATGTT+TGG 0.375732 6.1:-67776408 None:intergenic
TAATCTTCAATTTAGGAACT+TGG 0.378334 6.1:+67779763 MS.gene42496:CDS
GGCAACCATTGCTCATGCTT+TGG 0.379572 6.1:+67778061 MS.gene42496:intron
TAGGCCTAATCCTGGTTTCT+GGG 0.387526 6.1:+67774861 MS.gene42496:CDS
TCCTTAATAGCTTCTAAACT+TGG 0.390784 6.1:+67777694 MS.gene42496:CDS
TGTGAGCAGGTTTAGAGAAT+GGG 0.396668 6.1:+67776269 MS.gene42496:intron
ATTACCGACAACAGGTTGAA+TGG 0.400819 6.1:-67772488 None:intergenic
CTTTGCTTCTTCTCAGTGAA+AGG 0.403582 6.1:-67778477 None:intergenic
CGATTAGATCAGCTGTGAAC+TGG 0.406817 6.1:-67778151 None:intergenic
TCTTGGGGTACCTTAGGAAT+TGG 0.407309 6.1:-67780130 None:intergenic
AAAACCTGGCCTCCAGTGTC+TGG 0.410753 6.1:-67776477 None:intergenic
GAACAAGAGACTAAGAAGTT+TGG 0.412609 6.1:+67778963 MS.gene42496:CDS
CATTGCTTTGCAGGCAAGAA+AGG 0.413703 6.1:+67780105 MS.gene42496:intron
TTCCGTAAATTACCGACAAC+AGG 0.416211 6.1:-67772496 None:intergenic
ATTGATTCATCTCCATTATG+AGG 0.417122 6.1:-67779318 None:intergenic
AGAGAATGGGGATTTGATAA+GGG 0.418726 6.1:+67776282 MS.gene42496:CDS
TTGTGAGCAGGTTTAGAGAA+TGG 0.423330 6.1:+67776268 MS.gene42496:intron
ATTATAATCGATGGTGTCTC+AGG 0.424569 6.1:+67779282 MS.gene42496:CDS
GCAGATGATTTGCAAGTAGA+TGG 0.428936 6.1:+67774901 MS.gene42496:CDS
GTAGCAGCAATACCACTATA+TGG 0.432332 6.1:+67772810 MS.gene42496:CDS
AAATTATACTGATGGAAACT+TGG 0.433245 6.1:+67777666 MS.gene42496:CDS
ATGAACATAAAAGCAATTCC+TGG 0.435819 6.1:-67781012 None:intergenic
CGTCTGATACCGAATGCAAA+AGG 0.436501 6.1:+67777331 MS.gene42496:CDS
ATCGAATCGCGACACCCATT+GGG 0.438913 6.1:-67777446 None:intergenic
CCTTGGTTTGTTGCAAAAGT+TGG 0.440752 6.1:-67779246 None:intergenic
AGTTCTGATCTTCATCTGCT+TGG 0.440936 6.1:-67772762 None:intergenic
TATCGAATCGCGACACCCAT+TGG 0.442961 6.1:-67777447 None:intergenic
TGGGTGGTAATGATGCCAAC+TGG 0.445422 6.1:-67780246 None:intergenic
CAGTGGTGAATCTTCAAGTA+AGG 0.447365 6.1:+67780936 MS.gene42496:intron
AATGGGGATTTGATAAGGGC+TGG 0.447897 6.1:+67776286 MS.gene42496:CDS
CGATGCATCGCGGATACAAA+AGG 0.447960 6.1:+67779153 MS.gene42496:CDS
CTGCTACACAAGTTCTGTCT+TGG 0.450039 6.1:-67772794 None:intergenic
ATACTTGAAATACAAGGAAA+TGG 0.459100 6.1:+67774939 MS.gene42496:CDS
CGAAGAACTACTTTATAGTA+AGG 0.460157 6.1:+67778524 MS.gene42496:CDS
ATTGCCAGACACTGGAGGCC+AGG 0.463699 6.1:+67776473 MS.gene42496:CDS
GCAAACAAAGTGTTGAATAT+GGG 0.464065 6.1:+67779665 MS.gene42496:CDS
ATGGGGATTTGATAAGGGCT+GGG 0.465930 6.1:+67776287 MS.gene42496:CDS
TTTGCTGTTAGGCCTAATCC+TGG 0.466122 6.1:+67774853 MS.gene42496:CDS
GGAGCAGCAATGTTGAACTT+TGG 0.467131 6.1:-67778429 None:intergenic
TATTCCCAGAAACCAGGATT+AGG 0.467371 6.1:-67774865 None:intergenic
CACACTTCATTTACCATGCC+GGG 0.467909 6.1:+67778369 MS.gene42496:CDS
TTATTTAGAAAGATTTGCTC+AGG 0.470017 6.1:+67777477 MS.gene42496:CDS
GGACTTTGTCGCGTAGTCTC+AGG 0.472253 6.1:+67778390 MS.gene42496:CDS
TGTACTAGTTATGATAAAAT+CGG 0.474553 6.1:-67778186 None:intergenic
TAATTTCCAATTATGAGGTC+GGG 0.474736 6.1:-67777649 None:intergenic
AGTACATACCAAGAAATTGC+TGG 0.475992 6.1:+67778203 MS.gene42496:CDS
AAGTTCAACATTGCTGCTCC+AGG 0.484663 6.1:+67778432 MS.gene42496:CDS
TAAAGTAGTTCTTCGATGGC+AGG 0.487100 6.1:-67778516 None:intergenic
CAGTATAATTTCCAATTATG+AGG 0.488687 6.1:-67777654 None:intergenic
CGTAAACACAAGCCATATAG+TGG 0.489912 6.1:-67772822 None:intergenic
TAGACAGATTGATCGGCTCC+TGG 0.491103 6.1:-67778450 None:intergenic
TGGAACATAGTTTCTAAAGC+TGG 0.492132 6.1:+67779390 MS.gene42496:CDS
TCTTCTACAACGTGGCTAGC+TGG 0.494244 6.1:-67772866 None:intergenic
TTTGCTTCTTCTCAGTGAAA+GGG 0.494336 6.1:-67778476 None:intergenic
AACTGCAAGGTCAGTTCAGA+TGG 0.495963 6.1:+67779088 MS.gene42496:CDS
CCATCGATTATAATTTCAGC+TGG 0.497239 6.1:-67779273 None:intergenic
TTCCCTTCTCTGTCCAGAAA+GGG 0.498281 6.1:-67777417 None:intergenic
GGAGTTGTTGACATTGGTTG+AGG 0.498281 6.1:-67780154 None:intergenic
CTGGGGAAACACTGCAGGAA+GGG 0.509076 6.1:+67776305 MS.gene42496:CDS
ATAATTTCCAATTATGAGGT+CGG 0.509438 6.1:-67777650 None:intergenic
TTCTAAACTTGGTGTAACTC+AGG 0.509620 6.1:+67777705 MS.gene42496:CDS
AATCCGTTGGTTGTTCGAAA+GGG 0.514041 6.1:+67772642 MS.gene42496:CDS
GAAACCCATAATCTTCTCAA+TGG 0.514548 6.1:+67778888 MS.gene42496:CDS
TTTGGAACATTTATTCAGAC+GGG 0.515747 6.1:+67781056 MS.gene42496:CDS
GTGAGCAGGTTTAGAGAATG+GGG 0.518841 6.1:+67776270 MS.gene42496:intron
GTTGAACAAAGAACAAAAGT+TGG 0.520029 6.1:+67779712 MS.gene42496:CDS
ATTCCCTTCTCTGTCCAGAA+AGG 0.522424 6.1:-67777418 None:intergenic
AAGATTCTTGAACTTATGGA+TGG 0.523540 6.1:+67777622 MS.gene42496:CDS
ATAATTGGAAATTATACTGA+TGG 0.524931 6.1:+67777658 MS.gene42496:CDS
CTTTCTGAGGTACTAGAAGC+AGG 0.528208 6.1:+67776348 MS.gene42496:CDS
TCTTCGCAAACCACTCGACT+AGG 0.528585 6.1:-67778942 None:intergenic
GAATCCGTTGGTTGTTCGAA+AGG 0.530039 6.1:+67772641 MS.gene42496:CDS
TTGTGCTGCATCATGCCCCT+TGG 0.530931 6.1:-67780200 None:intergenic
AGCAGATGAAGATCAGAACT+TGG 0.532017 6.1:+67772765 MS.gene42496:CDS
GTCTTCAAAGAATCAATGAA+TGG 0.533715 6.1:+67779412 MS.gene42496:CDS
ATCTTTATTCTGGATTACGT+TGG 0.533971 6.1:+67780983 MS.gene42496:CDS
CTGCCCTTTCGAACAACCAA+CGG 0.534760 6.1:-67772645 None:intergenic
TTGTATTTCAAGTATTCTGA+AGG 0.535677 6.1:-67774931 None:intergenic
AATGATGCCAACTGGGTTTG+AGG 0.536634 6.1:-67780238 None:intergenic
AAGGGCTGGGGAAACACTGC+AGG 0.541174 6.1:+67776300 MS.gene42496:CDS
GTTCTAGGATTGCCAGACAC+TGG 0.541620 6.1:+67776465 MS.gene42496:CDS
TCACACTTCATTTACCATGC+CGG 0.542527 6.1:+67778368 MS.gene42496:CDS
CCAAAAGCTTCATACAATGC+TGG 0.542812 6.1:-67779189 None:intergenic
GAAGGGAATTCTATCCCAAT+GGG 0.543801 6.1:+67777432 MS.gene42496:CDS
GTGTGAGTTTCATATTGTCC+TGG 0.544414 6.1:-67778351 None:intergenic
GTCCCTTTCTGGACAGAGAA+GGG 0.546251 6.1:+67777415 MS.gene42496:CDS
GACATTGGTTGAGGTTCTTG+GGG 0.549444 6.1:-67780145 None:intergenic
ACTATAAAGTAGTTCTTCGA+TGG 0.550592 6.1:-67778520 None:intergenic
ATTTGGAACATTTATTCAGA+CGG 0.552070 6.1:+67781055 MS.gene42496:CDS
CTCTCTCATAAAATGAACAT+GGG 0.552785 6.1:+67772460 None:intergenic
TTTGTATCATCAGCAAGGAT+AGG 0.554135 6.1:+67778328 MS.gene42496:intron
AGTCCCTTTCTGGACAGAGA+AGG 0.554342 6.1:+67777414 MS.gene42496:CDS
CGAACAACCAACGGATTCTG+TGG 0.560672 6.1:-67772636 None:intergenic
AAAGCAGAAGCTACTTCCAA+GGG 0.561938 6.1:+67780184 MS.gene42496:CDS
AAAAGCAGAAGCTACTTCCA+AGG 0.562076 6.1:+67780183 MS.gene42496:CDS
TTGGCAGGAGTTGTTGACAT+TGG 0.562547 6.1:-67780160 None:intergenic
GAATCCATTCAACCTGTTGT+CGG 0.564794 6.1:+67772484 MS.gene42496:CDS
GTCTGATACCGAATGCAAAA+GGG 0.564856 6.1:+67777332 MS.gene42496:CDS
GGGTGGTAATGATGCCAACT+GGG 0.565407 6.1:-67780245 None:intergenic
AAGAAAGGTACCAATTCCTA+AGG 0.565800 6.1:+67780120 MS.gene42496:CDS
TGGGGATTTGATAAGGGCTG+GGG 0.568733 6.1:+67776288 MS.gene42496:CDS
TCTCTCTCATAAAATGAACA+TGG 0.569936 6.1:+67772459 None:intergenic
AAATGGTATTTGATGAGAAG+TGG 0.572044 6.1:+67774956 MS.gene42496:CDS
ATCATCAGCAAGGATAGGCC+AGG 0.575526 6.1:+67778333 MS.gene42496:intron
GAGACTACGCGACAAAGTCC+CGG 0.575633 6.1:-67778387 None:intergenic
CCAGCTGAAATTATAATCGA+TGG 0.576849 6.1:+67779273 MS.gene42496:CDS
AGAAGGGAATTCTATCCCAA+TGG 0.579688 6.1:+67777431 MS.gene42496:CDS
GCTGGGGAAACACTGCAGGA+AGG 0.584577 6.1:+67776304 MS.gene42496:CDS
TTAACATATTCCCAGAAACC+AGG 0.584706 6.1:-67774871 None:intergenic
TCTTCAAGTAAGGAACTAGC+TGG 0.586957 6.1:+67780946 MS.gene42496:CDS
CAAAGAACAAAAGTTGGCAA+AGG 0.588238 6.1:+67779718 MS.gene42496:CDS
GCAAGACTAGTCCTAAACAT+TGG 0.590830 6.1:+67779370 MS.gene42496:CDS
CCATAATCTTCTCAATGGCA+AGG 0.595223 6.1:+67778893 MS.gene42496:CDS
TTGAATCTAAGCTAACATCA+AGG 0.597124 6.1:+67780272 MS.gene42496:CDS
TAATTTCAGCTGGACCACCT+TGG 0.601700 6.1:-67779263 None:intergenic
AGGGAAGTAGACAGATTGAT+CGG 0.603416 6.1:-67778457 None:intergenic
GCTCGCACCACAGAATCCGT+TGG 0.609305 6.1:+67772629 MS.gene42496:CDS
TTCAGAATACTTGAAATACA+AGG 0.609609 6.1:+67774933 MS.gene42496:CDS
CTCCAAAAGATTGAGTTGCA+AGG 0.616584 6.1:+67777078 MS.gene42496:CDS
TGTGAAGCCTCAGATTCTTG+TGG 0.617074 6.1:+67777107 MS.gene42496:CDS
TTTCTCCAAAGCATGAGCAA+TGG 0.620352 6.1:-67778066 None:intergenic
ATGAAAGAGTACAAACTGCA+AGG 0.621541 6.1:+67779075 MS.gene42496:CDS
TGAAGTACCTCAAACCCAGT+TGG 0.633135 6.1:+67780231 MS.gene42496:CDS
AGGTCAGTTCAGATGGATTG+CGG 0.634342 6.1:+67779095 MS.gene42496:CDS
GAACTAGCTGGTAAACAAAG+TGG 0.635858 6.1:+67780958 MS.gene42496:CDS
TACGCGACAAAGTCCCGGCA+TGG 0.643567 6.1:-67778382 None:intergenic
ACATCAGCGGCCTAGTCGAG+TGG 0.646877 6.1:+67778932 MS.gene42496:CDS
GACAATGAGAATGCTTTCTG+AGG 0.650408 6.1:+67776335 MS.gene42496:CDS
TAGCATACCACAAGAATCTG+AGG 0.652849 6.1:-67777114 None:intergenic
CATACCAAGAAATTGCTGGA+AGG 0.669178 6.1:+67778207 MS.gene42496:CDS
CTAGGATTGCCAGACACTGG+AGG 0.672637 6.1:+67776468 MS.gene42496:CDS
ACTAGTTATGATAAAATCGG+CGG 0.672703 6.1:-67778183 None:intergenic
GACAAAGTGAAGAACATCAG+CGG 0.675713 6.1:+67778919 MS.gene42496:CDS
TGGAGATGAATCAATCAACA+AGG 0.681896 6.1:+67779326 MS.gene42496:CDS
TTGTTATGCAGAATGTCTAG+CGG 0.684403 6.1:+67772604 MS.gene42496:intron
GTGATTGAGGCAATGAACTG+TGG 0.684673 6.1:+67779219 MS.gene42496:CDS
CAAACTGATAGATATCGCAA+CGG 0.722489 6.1:+67779120 MS.gene42496:CDS
AAGCAGAAGCTACTTCCAAG+GGG 0.733208 6.1:+67780185 MS.gene42496:CDS
AGAGCTTTACCGATGCATCG+CGG 0.787941 6.1:+67779143 MS.gene42496:CDS
ATACAGTGTCTTCTACAACG+TGG 0.836634 6.1:-67772874 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content


Chromosome Type Strat End Strand Name
chr6.1 gene 67772472 67781086 67772472 ID=MS.gene42496
chr6.1 mRNA 67772472 67781086 67772472 ID=MS.gene42496.t1;Parent=MS.gene42496
chr6.1 exon 67772472 67772505 67772472 ID=MS.gene42496.t1.exon1;Parent=MS.gene42496.t1
chr6.1 CDS 67772472 67772505 67772472 ID=cds.MS.gene42496.t1;Parent=MS.gene42496.t1
chr6.1 exon 67772615 67772666 67772615 ID=MS.gene42496.t1.exon2;Parent=MS.gene42496.t1
chr6.1 CDS 67772615 67772666 67772615 ID=cds.MS.gene42496.t1;Parent=MS.gene42496.t1
chr6.1 exon 67772733 67772892 67772733 ID=MS.gene42496.t1.exon3;Parent=MS.gene42496.t1
chr6.1 CDS 67772733 67772892 67772733 ID=cds.MS.gene42496.t1;Parent=MS.gene42496.t1
chr6.1 exon 67774823 67774977 67774823 ID=MS.gene42496.t1.exon4;Parent=MS.gene42496.t1
chr6.1 CDS 67774823 67774977 67774823 ID=cds.MS.gene42496.t1;Parent=MS.gene42496.t1
chr6.1 exon 67776278 67776494 67776278 ID=MS.gene42496.t1.exon5;Parent=MS.gene42496.t1
chr6.1 CDS 67776278 67776494 67776278 ID=cds.MS.gene42496.t1;Parent=MS.gene42496.t1
chr6.1 exon 67777033 67777128 67777033 ID=MS.gene42496.t1.exon6;Parent=MS.gene42496.t1
chr6.1 CDS 67777033 67777128 67777033 ID=cds.MS.gene42496.t1;Parent=MS.gene42496.t1
chr6.1 exon 67777325 67777498 67777325 ID=MS.gene42496.t1.exon7;Parent=MS.gene42496.t1
chr6.1 CDS 67777325 67777498 67777325 ID=cds.MS.gene42496.t1;Parent=MS.gene42496.t1
chr6.1 exon 67777610 67777726 67777610 ID=MS.gene42496.t1.exon8;Parent=MS.gene42496.t1
chr6.1 CDS 67777610 67777726 67777610 ID=cds.MS.gene42496.t1;Parent=MS.gene42496.t1
chr6.1 exon 67778062 67778228 67778062 ID=MS.gene42496.t1.exon9;Parent=MS.gene42496.t1
chr6.1 CDS 67778062 67778228 67778062 ID=cds.MS.gene42496.t1;Parent=MS.gene42496.t1
chr6.1 exon 67778341 67778565 67778341 ID=MS.gene42496.t1.exon10;Parent=MS.gene42496.t1
chr6.1 CDS 67778341 67778565 67778341 ID=cds.MS.gene42496.t1;Parent=MS.gene42496.t1
chr6.1 exon 67778867 67778999 67778867 ID=MS.gene42496.t1.exon11;Parent=MS.gene42496.t1
chr6.1 CDS 67778867 67778999 67778867 ID=cds.MS.gene42496.t1;Parent=MS.gene42496.t1
chr6.1 exon 67779060 67779433 67779060 ID=MS.gene42496.t1.exon12;Parent=MS.gene42496.t1
chr6.1 CDS 67779060 67779433 67779060 ID=cds.MS.gene42496.t1;Parent=MS.gene42496.t1
chr6.1 exon 67779646 67779784 67779646 ID=MS.gene42496.t1.exon13;Parent=MS.gene42496.t1
chr6.1 CDS 67779646 67779784 67779646 ID=cds.MS.gene42496.t1;Parent=MS.gene42496.t1
chr6.1 exon 67780118 67780293 67780118 ID=MS.gene42496.t1.exon14;Parent=MS.gene42496.t1
chr6.1 CDS 67780118 67780293 67780118 ID=cds.MS.gene42496.t1;Parent=MS.gene42496.t1
chr6.1 exon 67780939 67781086 67780939 ID=MS.gene42496.t1.exon15;Parent=MS.gene42496.t1
chr6.1 CDS 67780939 67781086 67780939 ID=cds.MS.gene42496.t1;Parent=MS.gene42496.t1
Gene Sequence

>MS.gene42496

ATGAACATGGGAGAATCCATTCAACCTGTTGTCGGTAATTTACGGAACTTTTCTCCATCCAAAACTAACCATTTACTTATTCTTTGACTGTACATTGTCAGGAAACCATCCGTAGTCATCTGGCTACTCACATTGTTATGCAGAATGTCTAGCGGTTGCTCGCACCACAGAATCCGTTGGTTGTTCGAAAGGGCAGTGAGTAATGCTATGCTTTGCAGCTCAGCTATACTGGTTACAGAGTTTTGAAAATTTCAATCTCAGTACACAAATTGGAATCCTTATAGAAAGAGCCAAGCAGATGAAGATCAGAACTTGGAAGTTTCCAAGACAGAACTTGTGTAGCAGCAATACCACTATATGGCTTGTGTTTACGAACCATATTTTTGTGTTCTGTCCAGCTAGCCACGTTGTAGAAGACACTGTATGACTGTACATAGTAGAAAGTCTCTTTTTGTTTTAATCCATCTCGATCTTTTTCCTTTCAAGAATACAAGAGTTTGTACATATTGTGATACCGACAGATGGTTCATCACAGTGTCTGTTCAATGATATCAACTTCCATTTCAAAAAGTTGGATGCTTAGTTTATATTTTTTAATTTTTCAGTTCAAGTTTTTGATTATAAGTTACCAGAATATTATATTGTAGTATTTCGATGTAATGTATCATGTATGTAGAATTTCAAAACAAGTTCAGTCACATTCTAAAGAGAAATTTCAAATATGTGTTTGTATTCTAAGGAGTATGTCTGAGGAAAATTCCTATGTCTCATAAAAGAAATTTCCTTATGGATTTGATTGTTCATCACTGCATTTGAGTCTCATGAAAGTCCTATTGCTGATCATGCTGTCACATGGATTACTTTCTGCATGTAGAGTTCTCTGAGTTAAAGGCACCATGTTTACTTAGGAGGCAATATTATGGTTATTGTGTTGGCTTCCAATCTTTCCTATATCTTATTTCATCAGAATTTAAGTACTTCAGTCAATTATAGTAACTAAACCTAACAGACTTACTTAACGGGCCACGTATGAAAAATAAAAGTAACTAAACATATTACTATTTACACTATTATATACAACAGGTAGTAAATTTGCTTTATTCACAATCTGAATCTGTTCATGTTATTCACAATTTTTACAGAATAACCTATGTTACTAGAGGTTAAGTTTCCAATAATTTAATGTAAAGTTGTGCCTTCATAATGTGTTTGGCTGTTAAAACTTAAATCAGGGGCCAGCTATAGAGGTAATTTAATATTAGTTGCATCAGAGAATGAAGGAACTCAAATGACTCAGTTATAAGAAATTCATTATTCTAAGTACAACTAGATCACTCCTAGGCTGATAGTTCTGTTCAATATATATGTTAACTAACAACCCCCAATAACTTCATACCAAAAACTGGACTAGAAAAATATGATTGGTGGACTAGAAAAATGACTCAGACTCGGTGATATTTTTTTTATGAAAAAAAAAATATTTTTTCTCAAAAAATTTAAAACAAACGCACATATGATTTTTGTTGAAAGGACATAGTTGTTATAACTAAATTCCTTATATTATATTGAAATTTGGATACGTTTTTGTTCCTATTATTATGGTTATTTTTTGGTCTAAATTTAGTTTTTTAATACATGTTATTTTGATATATAATATTTAACAAAGTATTTTACACTAGTTTACAAATGCTAGCTTAACTAACAAAAGTGCGGGAATTGTTAGGTCACACGTCGTGACTATGGTTCAAACTCAGACTTCTTCTTAGATGTCGTGAAATTACTAATTTTAGTTTGTTTATATAAAAAAAAATCTTTTATTCTATAAGTATGTTTTTTTTAGTTTGTTTATATACAAAAAAAAAGTCTTTTATACTATAAATACATTTTAAATTAGTAATTTCATTTTCATCAAAGTGTCATTTGAATACTTTTTTATTTTATTTTTGTTTAATTGTTGTTTTAATGGTTTAAAGATACAATTTGTCAAGTTAACCATTTTTTCAATTATTTTCAATAAAACTAAAAAGAGTTTGTTTTTGTTATGTGAAAATTCAAACTTCATTGAAACTAAATTCGATCCTAATAATAGTTTTTTGTTTGAAGGATCAATCCATATAATGATGATTCGTGAGAGTCGATCCTTATAATAATGATTCATTGAAAAAGGCTTCTCTCAAAAAAAATAAAAATAAAAAAGGCTATAATAGAAAATGAACAAAGAAAAAAAAAGCTAACTTATGTCTTTAATACACATGTTAATGAGTTAAAAATGAGAGGAAACAATTGAAGATAAAAATGAGAGGAAAAAGCTTTTGGAGGGCTTATTCGGATACATCCTAAGTTGTACACAGGAGGCAGCTATTGTTCCACCATATGTTGTTTTTGCTGTTAGGCCTAATCCTGGTTTCTGGGAATATGTTAAAGTGAATGCAGATGATTTGCAAGTAGATGGTATTGAGCCTTCAGAATACTTGAAATACAAGGAAATGGTATTTGATGAGAAGTGGTAAGTTGTATTCTTAATTTGAAACTCATTTGCATAGTTGATTTTGGCTAGGCATGCAATTTTATTTGGAGTAAATAAGCATTTTAGTCCTCGAAATTGTAAACATAAGGCAATTTTGTCCCTCAAATTTACAAAACAGATTAGTCGTACGTCAAAATCAAAATATCTCATTAATAAACATCTACTAAAACTAATGAAAGACTTACATATCTAACTTGAATAGTCTGACGCTGCATCAAAATAAGTATAAAATTTTCAAAACTGTACGGTTAAATTTGAAAAAAAGACTAGATTGATTGACGTTCAAATTAAGGGATTAAATTGCTATTTTGCAAATTTCAAACTAAATTTACTGATCCTTATAATTTGAAGGACTAAAATGCTAATGTACTCATTTACTACGTGTCGATATTTATTTGACTCTTTGATAAGGCAAATGGTCCATGAAATGTTAGTTCCATGGCAAGAGCGTGACCGTAAGGGCCGAAGTTCAAATGTCTTTGGAGACAATTATAAAATGACTATTAGTGCTACTTTATTATCCCTTACTATTTTTTTTTTCATTATAAAAAATGGTTAACATGAGTTGAATTTGCAGGGCAAGTGATGAGAATGCTTTAGAGATAAGATTTTGAGGCTATTGATTTCACCACACCTCATACGGAACTTTCATCTTCTATTGGAAAAGGACTTGACTTAACTACTAGGATCTTGACCTCAAGGTTGAGTGAGAGTCCTTGTTCAAAAAAAGGTTGAGTGAGAGTTCACACTGTGAAAATCCATTGCTTAATTACTTATTAAGCCTTAATCATCAAGGAGAGGTAATTAAAGCCATAAGGGTATCAAGTATTGAAATAAATCTGTACAATTGAAAAAAAAAATGAAGTTAATTATGAAAATAAAAATTCTATATTGTTTTTTTTTTTTTTACTATATTGCAGAATCTTATGATCAAAGACACTTTGAATACAATACCAAAACTTCAGAAAGCATTGAAAATAGCAGAATCATATGTATCTGCACATCACAAAGACACACCATACTAGAATTTTGAAAACAGGTATATATCTAACTCTTTTTCATAATCAAATTCTTATTATGAAGCGCTGACAATGACATGGACACTAGACACGACACTGCCACGTTGACACTGACAATGATCTTAAAAAATGAAACAACTGAACGTTACCAAATGTTTCCGTGTCTTGTCAGCGTACGGACACACTTTTAATCGGAAGTGTCAGTATACTATTAATATATACTAATACTTAGTATTATGATTTTTGTTTTTTGTGAGCAGGTTTAGAGAATGGGGATTTGATAAGGGCTGGGGAAACACTGCAGGAAGGGTAAAAGAGACAATGAGAATGCTTTCTGAGGTACTAGAAGCAGGAGATCCAATTAAATTGGAATCACTTTTCAGCAGGCTACCAAACATGTTCAACATTGTTATTTTCTCTATTCATGGATACTTTGGTCAAGCAGATGTTCTAGGATTGCCAGACACTGGAGGCCAGGTTTTAATCCTTCCTTATTGGTTCCTAAATACCATATTAATTTATCTAACTACTTCTCTCAATAGGAACATTTTATGGTGTACCGTCTCGACATGTGGTCCACCGTAGACCAGTATTTGAAAGCCGTCCACAATTCCAATAATAATGGACGACGTTTCCAGAAGCAGAAGCTCACGACCCTCCTTGTTGCAACATCCGATACATTGACATAATCGGTGCCAGTTTGGCAAAAATATGAGAAAACAAATATGTATTTTGCAAAACTCATCAGATATTTGTTTTCTCTTGTTTTTGTCAAACTGACACCAATCAAGTCAAAGTCTCAGTCGTGAGGACAAAGAGGACTGTGAACTTCTACTTTTGGAAGACCGTGTTTTGTATTTTGAGCGGTTTTCAAGTACTAGTTCACAATGGAACATGCCCTCTTCAATATAACTAAAATTTGTCAAAATTATGGTGAATTTGTCTAGTTGTGTAACACTATATAACATTGGAAATTTGAAAAATTGAATAAATTTTTCTTTATTGGGACTAGCAGGTGGTATACATTCTTGATCAAGTAAGAGCATTAGAAGAAGAGCTACTCCAAAAGATTGAGTTGCAAGGCCTTAATGTGAAGCCTCAGATTCTTGTGGTATGCTACCAAGTACTAATACAAAAAATTACAAATAATGTCTACCAAATTTTCTTTTACAAATTATTATACTTCAATTTTCTTCTGTTTCCTCTCTATATAATGCCTTTGCTCATATGTTTCACCGGCATGCAGATGAAGACTTACGTATTTGTCTATTCCGTTTTTTTATTGCGCTAATGTGTTGCATGCACAGGTTACACGTCTGATACCGAATGCAAAAGGGACAACATGTAATCAAGAATTTGAACCTATCATCAAAACTAAGCATTCACACATTCTTAGAGTCCCTTTCTGGACAGAGAAGGGAATTCTATCCCAATGGGTGTCGCGATTCGATATATATCCTTATTTAGAAAGATTTGCTCAGGCATGTAGAATCATATCTTCATTTGATTTCCCTCTCTTCCATATTTGATATTACTATCAAGATTATGCATTTTAGTTCCTTTTTCTAATTATGCATTTTTATTATAAACAGGATTCTACTACCAAGATTCTTGAACTTATGGATGGAAAACCCGACCTCATAATTGGAAATTATACTGATGGAAACTTGGTTTCATCCTTAATAGCTTCTAAACTTGGTGTAACTCAGGTTAGCTTATGGATAAACACCTTTATTAAGTGCTTATATAATATAAGTGCTTATGTACAAGATATTTCTATAATAAAAAATAAAAAAGTCAAAATGTTTAAGTTATAAGCTATTTTCAAAACTATTCTGGAGAGCTTATGTAAACAACTGAAAACAGCTTAAGACATGTCATAAGCTGTTTCCATAAACTCTTTGAAATAGTCTCATAAATGCTTACGTTAGTAGATAAACTCAAATAAGTCAATCAAGGAATATTCATCGGTATAAATATACATTCTTTTCCGATTACTCTATTAGTATTATTGAACTAAAACTTCTATCTATAATGATTGAAGGCAACCATTGCTCATGCTTTGGAGAAAACAAAATATGAAGATTCAGATGCTAAATGGAATAGTTTTGAAGAAAAGTATCACTTTTCAAGCCAGTTCACAGCTGATCTAATCGCAATGAATTCCGCCGATTTTATCATAACTAGTACATACCAAGAAATTGCTGGAAGGTTGACCTTTTTTCTTCTTCTTATAAATTATACATAAATTCTTATATACTATCTTATGGTTAATGTAATATTATCATGAAGGTAACATTTTAATTGTATTTTGTATCATCAGCAAGGATAGGCCAGGACAATATGAAACTCACACTTCATTTACCATGCCGGGACTTTGTCGCGTAGTCTCAGGCATCAATGTATTTGATCCAAAGTTCAACATTGCTGCTCCAGGAGCCGATCAATCTGTCTACTTCCCTTTCACTGAGAAGAAGCAAAGATTGATCACTTTTCAACCTGCCATCGAAGAACTACTTTATAGTAAGGTCGAAAATGAAGAACACATGTGAGTTTCCTCCTATTACTTTCTTATCAAGTTATCATCCACTCGCATATTATGGTAACCATAATATAAAATAGTTGAAGGATTAGGGTTTAAATTCTATCGTAGGAGAAAATACCAATATAACAGTTAATATACTAACATTTTGCATATAAAAAAAACATATGTTTGGATGATAAACTTGTTATGGATTCACATTGCTTTCTTAAACATTATTGACATGTTTCGATGTTATGCTTAGCTTTGGATCAATAAATCGTAACTAACTAAATTGTTTCGTCGAATAAAAATGTGTTTGGACCAGTGGATTTTTCGAAGACAAGAAGAAACCCATAATCTTCTCAATGGCAAGGCTTGACAAAGTGAAGAACATCAGCGGCCTAGTCGAGTGGTTTGCGAAGAACAAGAGACTAAGAAGTTTGGTAAATCTTGTAATTGTTGGCGGATTCTTCGATCCGTCAAAATCCAAAGATAGAGAAGAAACATAAGAAATCAAGAAAATGCATTACTTGATGAAAGAGTACAAACTGCAAGGTCAGTTCAGATGGATTGCGGCGCAAACTGATAGATATCGCAACGGAGAGCTTTACCGATGCATCGCGGATACAAAAGGAGCATTTGTTCAACCAGCATTGTATGAAGCTTTTGGTCTAACAGTGATTGAGGCAATGAACTGTGGATTACCAACTTTTGCAACAAACCAAGGTGGTCCAGCTGAAATTATAATCGATGGTGTCTCAGGTTTTCATATTGATCCTCATAATGGAGATGAATCAATCAACAAGGTTTCTGAATTTTTTGAGAAGTGCAAGACTAGTCCTAAACATTGGAACATAGTTTCTAAAGCTGGTCTTCAAAGAATCAATGAATGGTACATTTCTGATTTATGGTTATGGATATTATCGGCAAAACAATAGTTAATATCGTATTAAACTTTGAAAATGACAAATAAATCGGAAAACTTTTTTTTTATTTACGAAAGTGACAGTTAAAAAGGAACAGAGGGAGTAACGGATATGCGTAGTTTAACAAACATTCATATGGTTGGATTTCTTATCTTTATTTCTTGTGTTTTCTTTGTAGCTATACATGGAAGATATATGCAAACAAAGTGTTGAATATGGGATCAATTTATGGGTTTTGGAGAAAGTTGAACAAAGAACAAAAGTTGGCAAAGGAAAGATACATACAAATGTTTTATAATCTTCAATTTAGGAACTTGGTAAGTTTGATGTTATTTTTAATAGTTTTCATCGGTTTGTTACAAATTTTTAAAAAATGTAAATAAAAAAAAAGAAATTAAAAACTAATTCAAATGAGAATCTGTTTTGAGCCGTTTCTCTGGAAAATAATTTTTTTTATAAATGTTTTTCTTTTAAAAGATCGATTTTTATTTTGATAAATGAACACAAAATTGCTTCCGGTATTCTTGAAATCTCTAAAAACTAATCTCGGTATTATCGAATACAAAAATCACATTTTTACAATATATAACAAATAGGCCTTAAATATAATAAGAATTGAAAAGTTATGGGAAAATTTCATTGCTTTGCAGGCAAGAAAGGTACCAATTCCTAAGGTACCCCAAGAACCTCAACCAATGTCAACAACTCCTGCCAAAAAAGCAGAAGCTACTTCCAAGGGGCATGATGCAGCACAAGCAAAAGCTGAAGTACCTCAAACCCAGTTGGCATCATTACCACCCAAAATTGAATCTAAGCTAACATCAAGGTAAGTCATTTATGCAAATAGCTATACCTCATTAGTCAAACTAAAAAATGATCTAAAAGTTACCCTAAATGAAAGCTTTGCATGTGTAAAAGAAAAATGACTGAATTACATTTTTCACCCCTAAATATTATATTATGATTGTGCATTTTCGGACTCAAGGTCTAGTTATGCAAATTGATTCTCACAAGTATCATTATTTTTACAGTTAAGTTCATCATTCACCGAACATTCCAATTTTTAACGTCACTAGCCTAAACGTCCAATGTGGCGAACGAGTGTTGCATATGACGACTCATATTTTGTTACTTGTCGCATGTCAATATTCTATAAGCTAGTTTGAGTCAGTGACAACTAAGATCCATGTGTGTCATGTAGGCTATTGAAGTTAAAATTCAACGACCTAGTTAGTGATCGAGGGACATGATTGTCACAAGATGGTTACTTAGAAACTTTGTTGGCACAATTAAAACTTTGAGACCGAAATTGCACAATCGTTATAATTATGCCCTAGGGACTATGCCAAAAAAATTAACATGGAAGAAATTTGCATGCAAAGTTAATAAATATAAAAATATATTTGATATGATTCAAATAAGAGAGAAAATTTTATAAATCTTTTGCATGTATTTGTCTTTGGATTTGCAGTGGTGAATCTTCAAGTAAGGAACTAGCTGGTAAACAAAGTGGTGATCTTTATTCTGGATTACGTTGGTTGCTTCCAGGAATTGCTTTTATGTTCATCATTCATTATCTCTCAAAGTATTTGGAACATTTATTCAGACGGGAGCAATAA
Protein sequence

>MS.gene42496.t1

MNMGESIQPVVECLAVARTTESVGCSKGHTQIGILIERAKQMKIRTWKFPRQNLCSSNTTIWLVFTNHIFVFCPASHVVEDTEAAIVPPYVVFAVRPNPGFWEYVKVNADDLQVDGIEPSEYLKYKEMVFDEKWFREWGFDKGWGNTAGRVKETMRMLSEVLEAGDPIKLESLFSRLPNMFNIVIFSIHGYFGQADVLGLPDTGGQVVYILDQVRALEEELLQKIELQGLNVKPQILVVTRLIPNAKGTTCNQEFEPIIKTKHSHILRVPFWTEKGILSQWVSRFDIYPYLERFAQDSTTKILELMDGKPDLIIGNYTDGNLVSSLIASKLGVTQATIAHALEKTKYEDSDAKWNSFEEKYHFSSQFTADLIAMNSADFIITSTYQEIAGSKDRPGQYETHTSFTMPGLCRVVSGINVFDPKFNIAAPGADQSVYFPFTEKKQRLITFQPAIEELLYSKVENEEHIGFFEDKKKPIIFSMARLDKVKNISGLVEWFAKNKRLRSLVNLVIKMHYLMKEYKLQGQFRWIAAQTDRYRNGELYRCIADTKGAFVQPALYEAFGLTVIEAMNCGLPTFATNQGGPAEIIIDGVSGFHIDPHNGDESINKVSEFFEKCKTSPKHWNIVSKAGLQRINECYTWKIYANKVLNMGSIYGFWRKLNKEQKLAKERYIQMFYNLQFRNLARKVPIPKVPQEPQPMSTTPAKKAEATSKGHDAAQAKAEVPQTQLASLPPKIESKLTSSGESSSKELAGKQSGDLYSGLRWLLPGIAFMFIIHYLSKYLEHLFRREQ