AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene05150

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene05150.t1 MTR_6g478000 88.913 911 30 3 1 910 1 841 0.0 1657
MS.gene05150.t1 MTR_5g076830 72.034 826 230 1 6 831 4 828 0.0 1292
MS.gene05150.t1 MTR_6g478030 96.970 627 19 0 132 758 894 1520 0.0 1275
MS.gene05150.t1 MTR_3g064610 70.000 830 245 2 5 830 3 832 0.0 1258
MS.gene05150.t1 MTR_4g124660 54.963 806 357 4 5 809 2 802 0.0 937
MS.gene05150.t1 MTR_7g108930 55.930 801 349 2 11 811 9 805 0.0 936
MS.gene05150.t1 MTR_2g044070 54.229 804 362 4 7 809 2 800 0.0 934
MS.gene05150.t1 MTR_1g088170 55.094 795 356 1 9 803 7 800 0.0 919
MS.gene05150.t1 MTR_4g124660 55.910 753 326 4 5 756 2 749 0.0 880
MS.gene05150.t1 MTR_6g478020 92.857 252 13 2 638 884 1 252 4.86e-158 496
MS.gene05150.t1 MTR_0277s0020 80.000 270 17 3 646 910 1159 1396 1.91e-132 431
MS.gene05150.t1 MTR_0277s0030 86.061 165 17 3 752 910 59 223 6.24e-89 282
MS.gene05150.t1 MTR_0277s0030 80.882 68 13 0 582 649 1 68 6.69e-29 115
MS.gene05150.t1 MTR_0419s0050 58.491 159 6 2 582 740 1 99 9.72e-51 173
MS.gene05150.t1 MTR_3g047380 25.430 523 322 16 285 757 167 671 1.09e-34 143
MS.gene05150.t1 MTR_5g091340 26.476 525 317 21 285 757 171 678 1.90e-34 142
MS.gene05150.t1 MTR_4g115620 25.260 578 343 21 265 779 125 676 4.14e-34 142
MS.gene05150.t1 MTR_0419s0010 94.828 58 3 0 746 803 12 69 3.07e-33 122
MS.gene05150.t1 MTR_6g081130 63.889 108 26 3 811 910 14 116 1.36e-31 119
MS.gene05150.t1 MTR_0419s0020 86.364 66 9 0 814 879 17 82 5.54e-30 114
MS.gene05150.t1 MTR_0419s0060 76.923 52 8 1 814 861 17 68 4.35e-17 76.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene05150.t1 AT1G73370 71.673 819 230 1 1 819 1 817 0.0 1267
MS.gene05150.t1 AT1G73370 71.673 819 230 1 1 819 1 817 0.0 1267
MS.gene05150.t1 AT1G73370 72.129 775 214 1 45 819 1 773 0.0 1202
MS.gene05150.t1 AT5G37180 68.045 798 252 1 12 809 5 799 0.0 1176
MS.gene05150.t1 AT5G37180 74.141 611 158 0 199 809 1 611 0.0 981
MS.gene05150.t1 AT4G02280 56.250 800 349 1 9 808 6 804 0.0 956
MS.gene05150.t1 AT5G49190 54.944 799 359 1 11 809 5 802 0.0 930
MS.gene05150.t1 AT5G49190 56.057 776 340 1 34 809 18 792 0.0 924
MS.gene05150.t1 AT5G37180 75.949 553 133 0 257 809 1 553 0.0 914
MS.gene05150.t1 AT5G20830 54.074 810 366 4 3 811 2 806 0.0 911
MS.gene05150.t1 AT5G20830 54.074 810 366 4 3 811 2 806 0.0 911
MS.gene05150.t1 AT5G20830 54.074 810 366 4 3 811 2 806 0.0 911
MS.gene05150.t1 AT5G49190 57.823 735 309 1 75 809 3 736 0.0 910
MS.gene05150.t1 AT3G43190 54.208 808 364 4 3 809 2 804 0.0 910
MS.gene05150.t1 AT3G43190 54.208 808 364 4 3 809 2 804 0.0 910
MS.gene05150.t1 AT4G10120 25.621 523 322 17 285 769 23 516 3.19e-35 145
MS.gene05150.t1 AT4G10120 25.621 523 322 17 285 769 197 690 5.95e-35 144
MS.gene05150.t1 AT4G10120 25.621 523 322 17 285 769 197 690 5.95e-35 144
MS.gene05150.t1 AT4G10120 25.621 523 322 17 285 769 197 690 5.95e-35 144
MS.gene05150.t1 AT1G04920 26.753 542 323 21 281 767 168 690 9.73e-35 144
MS.gene05150.t1 AT4G10120 25.253 495 319 14 304 769 17 489 1.07e-33 140
MS.gene05150.t1 AT5G11110 25.047 535 337 19 285 783 177 683 1.16e-33 140
MS.gene05150.t1 AT5G20280 25.414 543 328 20 281 778 166 676 2.13e-32 136
MS.gene05150.t1 AT1G78800 27.311 238 141 9 538 767 176 389 3.32e-11 67.0

Find 177 sgRNAs with CRISPR-Local

Find 0 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCATTATCTCTCAAAGTATT+TGG 0.129152 6.4:+51398979 MS.gene05150:CDS
TTGAATATGGGATCAATTTA+TGG 0.132045 6.4:+51397377 MS.gene05150:CDS
TTCTGTTTCTTCTCTATCTT+TGG 0.134203 6.4:-51396727 None:intergenic
AAACTTAGTTTCATCCTTAA+TGG 0.191212 6.4:+51395340 MS.gene05150:CDS
TTGACATTGGTTGAGGTTCT+TGG 0.199139 6.4:-51398093 None:intergenic
TGGGATCAATTTATGGGTTT+TGG 0.205721 6.4:+51397384 MS.gene05150:CDS
CAAAGCGGTGATCTTTATTC+TGG 0.207693 6.4:+51398914 MS.gene05150:CDS
CTTGCCATTGAGAAGATTAT+AGG 0.209492 6.4:-51396591 None:intergenic
GGCCTTGTAATTCAATCTTT+TGG 0.212592 6.4:-51394711 None:intergenic
TGCAAACAAAGTGTTGAATA+TGG 0.228861 6.4:+51397364 MS.gene05150:CDS
TACCAAGATTCTTGAACTTA+TGG 0.238387 6.4:+51395277 MS.gene05150:CDS
TTACCTCTCCTTGATGATTA+AGG 0.239757 6.4:-51393803 None:intergenic
TTAGGCCTAATCCAGGTTTC+TGG 0.246738 6.4:+51392932 MS.gene05150:CDS
TGTTAGCTTAGATTCAATTT+TGG 0.252429 6.4:-51398212 None:intergenic
ACATTCTTAGAGTCCCTTTC+TGG 0.252632 6.4:+51395063 MS.gene05150:CDS
ACCAAGTTTAGAAGCCATTA+AGG 0.255408 6.4:-51395354 None:intergenic
GGAAAACCTGACCTCATTAT+TGG 0.261160 6.4:+51395302 MS.gene05150:CDS
TTTGGTCAAGCTGATGTTCT+AGG 0.267299 6.4:+51394414 MS.gene05150:CDS
TCAACCTTCCAGCAATTTCT+TGG 0.273129 6.4:-51395887 None:intergenic
ATGGCACTTTCATCTTCTAT+TGG 0.281086 6.4:+51393690 MS.gene05150:CDS
TGACATTGGTTGAGGTTCTT+GGG 0.286219 6.4:-51398092 None:intergenic
AGCAGGAGATCCAATTAAAA+TGG 0.288529 6.4:+51394329 MS.gene05150:CDS
TGAGCAAATCTTTCTAAATA+AGG 0.291781 6.4:-51395134 None:intergenic
GAAACTATGTTCCAATGTTC+AGG 0.294329 6.4:-51397080 None:intergenic
ATGAAGATTCAGATGCTAAA+TGG 0.297761 6.4:+51395772 MS.gene05150:CDS
TGATGATAGGTTCAAGTTCT+TGG 0.298390 6.4:-51395025 None:intergenic
CCAGCATTGTATGAAGCTTT+TGG 0.302612 6.4:+51396888 MS.gene05150:CDS
GGATTACGTTGGTTGCTTCC+AGG 0.317665 6.4:+51398935 MS.gene05150:CDS
TCCTTAATGGCTTCTAAACT+TGG 0.326870 6.4:+51395353 MS.gene05150:CDS
TTTCATATTGATCCTCATAA+TGG 0.327462 6.4:+51397005 MS.gene05150:CDS
TTAGTAAATCTTGTAATTGT+TGG 0.330790 6.4:+51396681 MS.gene05150:CDS
GAATCTGAGGCTTCACATTA+AGG 0.333564 6.4:-51394732 None:intergenic
TTCAATGCTTTCTGAAGCTT+TGG 0.336752 6.4:-51393970 None:intergenic
TAGAGAATGGGGATTTGATA+AGG 0.339743 6.4:+51394245 MS.gene05150:CDS
TGAGGTTCTTGGGGTACCTT+AGG 0.341726 6.4:-51398082 None:intergenic
CGGCTTTGTTTCAAGGCATC+AGG 0.345200 6.4:-51392114 None:intergenic
TTCTCTATTCATGGATACTT+TGG 0.345725 6.4:+51394396 MS.gene05150:CDS
TGGCAAAGCATTTCTTCATA+TGG 0.350848 6.4:-51392140 None:intergenic
TGAATATGGGATCAATTTAT+GGG 0.351676 6.4:+51397378 MS.gene05150:CDS
ATCCATAAGTTCAAGAATCT+TGG 0.355620 6.4:-51395279 None:intergenic
TAAAGTGAATGCAGATGATT+TGG 0.361708 6.4:+51392963 MS.gene05150:CDS
GGTCAATCTTTGCTTCTTCT+CGG 0.370209 6.4:-51396160 None:intergenic
ATTATAATTGATGGTGTCTC+AGG 0.371755 6.4:+51396981 MS.gene05150:CDS
TGAAGAATTACTTTATAGTA+AGG 0.375146 6.4:+51396200 MS.gene05150:CDS
ATAACAATGTTGAACATGTT+TGG 0.375732 6.4:-51394372 None:intergenic
TAATCTTCAATTTAGGAACT+TGG 0.378334 6.4:+51397463 MS.gene05150:CDS
GGCAACCATTGCTCATGCTT+TGG 0.379572 6.4:+51395737 MS.gene05150:intron
TGAAACAGGTTGGTTGCAAA+TGG 0.380181 6.4:+51392754 MS.gene05150:intron
AGAGAATGGGGATTTGATAA+GGG 0.380444 6.4:+51394246 MS.gene05150:CDS
ATGGAAACGGCTTTGTTTCA+AGG 0.384267 6.4:-51392121 None:intergenic
TGTGAGCAGGTTTAGAGAAT+GGG 0.396668 6.4:+51394233 MS.gene05150:intron
TGATTTCACCACACCTCATA+TGG 0.403948 6.4:+51393671 MS.gene05150:CDS
TCTTGGGGTACCTTAGGAAT+TGG 0.407309 6.4:-51398076 None:intergenic
AAAACCTGGCCTCCAGTGTC+TGG 0.410753 6.4:-51394441 None:intergenic
CATTGCTTTGCAGGCAAGAA+AGG 0.413703 6.4:+51398051 MS.gene05150:intron
CTTTGCTTCTTCTCGGTGAA+AGG 0.416303 6.4:-51396153 None:intergenic
ATTGATTCATCTCCATTATG+AGG 0.417122 6.4:-51397017 None:intergenic
TTGTGAGCAGGTTTAGAGAA+TGG 0.423330 6.4:+51394232 MS.gene05150:intron
CTTTCATCTTCTATTGGAAA+AGG 0.424148 6.4:+51393696 MS.gene05150:CDS
TAGGCCTAATCCAGGTTTCT+GGG 0.431601 6.4:+51392933 MS.gene05150:CDS
GCAGATGATTTGGAAGTAGA+TGG 0.435227 6.4:+51392973 MS.gene05150:CDS
ATGAACATAAAAGCAATTCC+TGG 0.435819 6.4:-51398953 None:intergenic
CCTTGGTTTGTTGCAAAAGT+TGG 0.440752 6.4:-51396945 None:intergenic
CGATGCATCGCGGATACAAA+AGG 0.447960 6.4:+51396852 MS.gene05150:CDS
TGGGTGGTAAGGATGCCAAC+TGG 0.452672 6.4:-51398192 None:intergenic
AATGGGGATTTGATAAGGGG+TGG 0.454037 6.4:+51394250 MS.gene05150:CDS
ATACTTGAAATACAAGGAAA+TGG 0.459100 6.4:+51393011 MS.gene05150:CDS
TAAAGTAATTCTTCAATAGC+AGG 0.459289 6.4:-51396192 None:intergenic
ATTGCCAGACACTGGAGGCC+AGG 0.463699 6.4:+51394437 MS.gene05150:CDS
GCAAACAAAGTGTTGAATAT+GGG 0.464065 6.4:+51397365 MS.gene05150:CDS
GGAGCAGCAATGTTGAACTT+TGG 0.467131 6.4:-51396105 None:intergenic
CAGGGGTGAATCTTCAAGTA+AGG 0.467197 6.4:+51398877 MS.gene05150:intron
TATTCCCAGAAACCTGGATT+AGG 0.468624 6.4:-51392937 None:intergenic
TTATTTAGAAAGATTTGCTC+AGG 0.470017 6.4:+51395136 MS.gene05150:CDS
TAATTTCCAATAATGAGGTC+AGG 0.474083 6.4:-51395308 None:intergenic
GGTTGGTTGCAAATGGGAAA+AGG 0.475033 6.4:+51392761 MS.gene05150:intron
AGTACATACCAAGAAATTGC+TGG 0.475992 6.4:+51395879 MS.gene05150:CDS
TATCAAAACGCGACACCCAT+TGG 0.476180 6.4:-51395106 None:intergenic
AAGGATGCCAACTGGGTTTG+AGG 0.477495 6.4:-51398184 None:intergenic
GTAAATCTTGTAATTGTTGG+AGG 0.478799 6.4:+51396684 MS.gene05150:CDS
AAGTTCAACATTGCTGCTCC+AGG 0.484663 6.4:+51396108 MS.gene05150:CDS
AGCATTTCTTCATATGGAAA+CGG 0.485069 6.4:-51392134 None:intergenic
TTTGCTGTTAGGCCTAATCC+AGG 0.486027 6.4:+51392925 MS.gene05150:CDS
ATCAAAACGCGACACCCATT+GGG 0.489567 6.4:-51395105 None:intergenic
TGGAACATAGTTTCTAAAGC+TGG 0.492132 6.4:+51397089 MS.gene05150:CDS
GGGTGGTAAGGATGCCAACT+GGG 0.493137 6.4:-51398191 None:intergenic
AACTGCAAGGTCAGTTCAGA+TGG 0.495963 6.4:+51396787 MS.gene05150:CDS
CTGCCTCCTGTGTACAACTT+AGG 0.496308 6.4:-51392879 None:intergenic
CCATCAATTATAATTTCAGC+TGG 0.497239 6.4:-51396972 None:intergenic
TTAACATATTCCCAGAAACC+TGG 0.497442 6.4:-51392943 None:intergenic
AGTGAGACTAAATCACATAA+TGG 0.498159 6.4:+51392786 MS.gene05150:CDS
TTCCCTTCTCTGTCCAGAAA+GGG 0.498281 6.4:-51395076 None:intergenic
GGAGTTCTTGACATTGGTTG+AGG 0.498281 6.4:-51398100 None:intergenic
AAGGGGTGGGGAAACACTGC+AGG 0.501630 6.4:+51394264 MS.gene05150:CDS
CACACTGCATTTACCATGCC+GGG 0.502984 6.4:+51396045 MS.gene05150:CDS
GGACTTTGTCGCGTAGTCTC+CGG 0.504452 6.4:+51396066 MS.gene05150:CDS
GACATTGGTTGAGGTTCTTG+GGG 0.505653 6.4:-51398091 None:intergenic
CTCCAAAAGATTGAATTACA+AGG 0.506047 6.4:+51394709 MS.gene05150:CDS
TTGGCAGGAGTTCTTGACAT+TGG 0.509655 6.4:-51398106 None:intergenic
ATATGAAGAAATGCTTTGCC+AGG 0.509721 6.4:+51392142 MS.gene05150:CDS
AAAGCAGAAGCTACTTCCAA+TGG 0.512805 6.4:+51398130 MS.gene05150:CDS
GAAACAGGTTGGTTGCAAAT+GGG 0.514671 6.4:+51392755 MS.gene05150:intron
TTTGCTTCTTCTCGGTGAAA+GGG 0.515058 6.4:-51396152 None:intergenic
TTATTAAGCCTTAATCATCA+AGG 0.516137 6.4:+51393795 MS.gene05150:CDS
TAGACAGATTGATCAGCTCC+TGG 0.517673 6.4:-51396126 None:intergenic
ATCATCAGCAAGGATAGGCC+AGG 0.517818 6.4:+51396009 MS.gene05150:intron
GTGAGCAGGTTTAGAGAATG+GGG 0.518841 6.4:+51394234 MS.gene05150:intron
GTTGAACAAAGAACAAAAGT+TGG 0.520029 6.4:+51397412 MS.gene05150:CDS
ATTCCCTTCTCTGTCCAGAA+AGG 0.522424 6.4:-51395077 None:intergenic
ATTATTGGAAATTACACTGA+TGG 0.522908 6.4:+51395317 MS.gene05150:CDS
AAGATTCTTGAACTTATGGA+TGG 0.523540 6.4:+51395281 MS.gene05150:CDS
CCAGCTGAAATTATAATTGA+TGG 0.523668 6.4:+51396972 MS.gene05150:CDS
TTTGTATCATCAGCAAGGAT+AGG 0.525118 6.4:+51396004 MS.gene05150:intron
GTGGGGAAACACTGCAGGAA+GGG 0.527757 6.4:+51394269 MS.gene05150:CDS
CTTTCTGAGGTACTAGAAGC+AGG 0.528208 6.4:+51394312 MS.gene05150:CDS
TTCTAAACTTGGTGTAACTC+AGG 0.528300 6.4:+51395364 MS.gene05150:CDS
TCTTCGCGAACCACTCGACT+AGG 0.529092 6.4:-51396641 None:intergenic
CTTAATAAGTAATTAAGCAA+TGG 0.529556 6.4:-51393780 None:intergenic
TGGGGATTTGATAAGGGGTG+GGG 0.530468 6.4:+51394252 MS.gene05150:CDS
ATCTTTATTCTGGATTACGT+TGG 0.533971 6.4:+51398924 MS.gene05150:CDS
TTGTATTTCAAGTATTCTGA+AGG 0.535677 6.4:-51393003 None:intergenic
ATGGGGATTTGATAAGGGGT+GGG 0.535734 6.4:+51394251 MS.gene05150:CDS
GAAACCTATAATCTTCTCAA+TGG 0.538316 6.4:+51396587 MS.gene05150:CDS
GAAGATGAAAGTGCCATATG+AGG 0.538755 6.4:-51393684 None:intergenic
AAGGACTTGACTTTACTACT+AGG 0.539536 6.4:+51393715 MS.gene05150:CDS
TGGATCAAATACATTGATGC+CGG 0.540309 6.4:-51396085 None:intergenic
GTTCTAGGATTGCCAGACAC+TGG 0.541620 6.4:+51394429 MS.gene05150:CDS
CCAAAAGCTTCATACAATGC+TGG 0.542812 6.4:-51396888 None:intergenic
GAAGGGAATTCTATCCCAAT+GGG 0.543801 6.4:+51395091 MS.gene05150:CDS
GAGAATGGGGATTTGATAAG+GGG 0.545192 6.4:+51394247 MS.gene05150:CDS
GTCCCTTTCTGGACAGAGAA+GGG 0.546251 6.4:+51395074 MS.gene05150:CDS
TTCAATAGCAGGTTGAAAAG+TGG 0.548599 6.4:-51396181 None:intergenic
GTGTGAGTTTCATATTGCCC+TGG 0.549755 6.4:-51396027 None:intergenic
AAATCACATAATGGAAGATG+TGG 0.551952 6.4:+51392795 MS.gene05150:CDS
AGTCCCTTTCTGGACAGAGA+AGG 0.554342 6.4:+51395073 MS.gene05150:CDS
TCACACTGCATTTACCATGC+CGG 0.555687 6.4:+51396044 MS.gene05150:CDS
TGATCAAGTAAGAGCACTAG+AGG 0.555960 6.4:+51394678 MS.gene05150:CDS
AAGCAGAAGCTACTTCCAAT+GGG 0.558108 6.4:+51398131 MS.gene05150:CDS
GTCTTGAAAGAATCAATGAA+TGG 0.561091 6.4:+51397111 MS.gene05150:CDS
AAGAAAGGTACCAATTCCTA+AGG 0.565800 6.4:+51398066 MS.gene05150:CDS
AAATGGTATTTGATGAGAAG+TGG 0.572044 6.4:+51393028 MS.gene05150:CDS
CTATAATCTTCTCAATGGCA+AGG 0.572371 6.4:+51396592 MS.gene05150:CDS
GAGACTACGCGACAAAGTCC+CGG 0.574380 6.4:-51396063 None:intergenic
CAGTGTAATTTCCAATAATG+AGG 0.574786 6.4:-51395313 None:intergenic
AGAAGGGAATTCTATCCCAA+TGG 0.579688 6.4:+51395090 MS.gene05150:CDS
GGTGGGGAAACACTGCAGGA+AGG 0.587059 6.4:+51394268 MS.gene05150:CDS
TTGTGCTGCATCATGCCCAT+TGG 0.587884 6.4:-51398146 None:intergenic
CAAAGAACAAAAGTTGGCAA+AGG 0.588238 6.4:+51397418 MS.gene05150:CDS
GCAAGACTAATCCTGAACAT+TGG 0.590830 6.4:+51397069 MS.gene05150:CDS
TAATTTCAGCTGGACCACCT+TGG 0.601700 6.4:-51396962 None:intergenic
ATTTGGAACATTTATTCACA+AGG 0.606453 6.4:+51398996 MS.gene05150:CDS
TTCAGAATACTTGAAATACA+AGG 0.609609 6.4:+51393005 MS.gene05150:CDS
TGAACTCTCACTCAATCTTG+AGG 0.610156 6.4:-51393745 None:intergenic
TTTGGAACATTTATTCACAA+GGG 0.615724 6.4:+51398997 MS.gene05150:CDS
TGTGAAGCCTCAGATTCTTG+TGG 0.617074 6.4:+51394738 MS.gene05150:CDS
ATGAAAGAGTACAAACTGCA+AGG 0.621541 6.4:+51396774 MS.gene05150:CDS
CTTCTCCAAAGCATGAGCAA+TGG 0.622761 6.4:-51395742 None:intergenic
TGAAGTACCTCAAACCCAGT+TGG 0.633135 6.4:+51398177 MS.gene05150:CDS
AGGTCAGTTCAGATGGATTG+CGG 0.634342 6.4:+51396794 MS.gene05150:CDS
TCATCAGCAAGGATAGGCCA+GGG 0.636461 6.4:+51396010 MS.gene05150:intron
TACGCGACAAAGTCCCGGCA+TGG 0.643567 6.4:-51396058 None:intergenic
GACAATGAGAATGCTTTCTG+AGG 0.650408 6.4:+51394299 MS.gene05150:CDS
TAGCATACCACAAGAATCTG+AGG 0.652849 6.4:-51394745 None:intergenic
CATCCTAAGTTGTACACAGG+AGG 0.653499 6.4:+51392876 MS.gene05150:CDS
ACTAGTTATGATAAAATCAG+CGG 0.654491 6.4:-51395859 None:intergenic
AAGCCTTAATCATCAAGGAG+AGG 0.663492 6.4:+51393800 MS.gene05150:CDS
ACATTGGAGGCCTAGTCGAG+TGG 0.664679 6.4:+51396631 MS.gene05150:CDS
CATACCAAGAAATTGCTGGA+AGG 0.669178 6.4:+51395883 MS.gene05150:CDS
CTAGGATTGCCAGACACTGG+AGG 0.672637 6.4:+51394432 MS.gene05150:CDS
TGGAGATGAATCAATCAACA+AGG 0.681896 6.4:+51397025 MS.gene05150:CDS
GTGATTGAGGCAATGAACTG+TGG 0.684673 6.4:+51396918 MS.gene05150:CDS
ATACATCCTAAGTTGTACAC+AGG 0.691450 6.4:+51392873 MS.gene05150:CDS
TGAAAGTGCCATATGAGGTG+TGG 0.718654 6.4:-51393679 None:intergenic
CAAACTGATAGATATCGCAA+CGG 0.722489 6.4:+51396819 MS.gene05150:CDS
GAACTAGCTGCTAAACAAAG+CGG 0.733394 6.4:+51398899 MS.gene05150:CDS
TTGAATCTAAGCTAACAACA+AGG 0.739018 6.4:+51398218 MS.gene05150:CDS
AGAGCTTTACCGATGCATCG+CGG 0.787941 6.4:+51396842 MS.gene05150:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content


Chromosome Type Strat End Strand Name
chr6.4 gene 51392057 51399027 51392057 ID=MS.gene05150
chr6.4 mRNA 51392057 51399027 51392057 ID=MS.gene05150.t1;Parent=MS.gene05150
chr6.4 exon 51392057 51392163 51392057 ID=MS.gene05150.t1.exon1;Parent=MS.gene05150.t1
chr6.4 CDS 51392057 51392163 51392057 ID=cds.MS.gene05150.t1;Parent=MS.gene05150.t1
chr6.4 exon 51392762 51393049 51392762 ID=MS.gene05150.t1.exon2;Parent=MS.gene05150.t1
chr6.4 CDS 51392762 51393049 51392762 ID=cds.MS.gene05150.t1;Parent=MS.gene05150.t1
chr6.4 exon 51393629 51393821 51393629 ID=MS.gene05150.t1.exon3;Parent=MS.gene05150.t1
chr6.4 CDS 51393629 51393821 51393629 ID=cds.MS.gene05150.t1;Parent=MS.gene05150.t1
chr6.4 exon 51393937 51394055 51393937 ID=MS.gene05150.t1.exon4;Parent=MS.gene05150.t1
chr6.4 CDS 51393937 51394055 51393937 ID=cds.MS.gene05150.t1;Parent=MS.gene05150.t1
chr6.4 exon 51394242 51394458 51394242 ID=MS.gene05150.t1.exon5;Parent=MS.gene05150.t1
chr6.4 CDS 51394242 51394458 51394242 ID=cds.MS.gene05150.t1;Parent=MS.gene05150.t1
chr6.4 exon 51394664 51394759 51394664 ID=MS.gene05150.t1.exon6;Parent=MS.gene05150.t1
chr6.4 CDS 51394664 51394759 51394664 ID=cds.MS.gene05150.t1;Parent=MS.gene05150.t1
chr6.4 exon 51394984 51395157 51394984 ID=MS.gene05150.t1.exon7;Parent=MS.gene05150.t1
chr6.4 CDS 51394984 51395157 51394984 ID=cds.MS.gene05150.t1;Parent=MS.gene05150.t1
chr6.4 exon 51395269 51395385 51395269 ID=MS.gene05150.t1.exon8;Parent=MS.gene05150.t1
chr6.4 CDS 51395269 51395385 51395269 ID=cds.MS.gene05150.t1;Parent=MS.gene05150.t1
chr6.4 exon 51395738 51395904 51395738 ID=MS.gene05150.t1.exon9;Parent=MS.gene05150.t1
chr6.4 CDS 51395738 51395904 51395738 ID=cds.MS.gene05150.t1;Parent=MS.gene05150.t1
chr6.4 exon 51396017 51396241 51396017 ID=MS.gene05150.t1.exon10;Parent=MS.gene05150.t1
chr6.4 CDS 51396017 51396241 51396017 ID=cds.MS.gene05150.t1;Parent=MS.gene05150.t1
chr6.4 exon 51396566 51397132 51396566 ID=MS.gene05150.t1.exon11;Parent=MS.gene05150.t1
chr6.4 CDS 51396566 51397132 51396566 ID=cds.MS.gene05150.t1;Parent=MS.gene05150.t1
chr6.4 exon 51397346 51397484 51397346 ID=MS.gene05150.t1.exon12;Parent=MS.gene05150.t1
chr6.4 CDS 51397346 51397484 51397346 ID=cds.MS.gene05150.t1;Parent=MS.gene05150.t1
chr6.4 exon 51398064 51398239 51398064 ID=MS.gene05150.t1.exon13;Parent=MS.gene05150.t1
chr6.4 CDS 51398064 51398239 51398064 ID=cds.MS.gene05150.t1;Parent=MS.gene05150.t1
chr6.4 exon 51398880 51399027 51398880 ID=MS.gene05150.t1.exon14;Parent=MS.gene05150.t1
chr6.4 CDS 51398880 51399027 51398880 ID=cds.MS.gene05150.t1;Parent=MS.gene05150.t1
Gene Sequence

>MS.gene05150

ATGGCTTCTACTTCAAGTTCTGCTTTGAAAAGATCTGATTCAATTGCTGATAGCATGCCTGATGCCTTGAAACAAAGCCGTTTCCATATGAAGAAATGCTTTGCCAGGTACTCCCTTTGTCTCATATTATTTGTTGTTTAAGGTTAGGTACAAGACATAACAAAAATCATGCATTACACTTATTAATTATCAAGGTAACATAATATTTTTGTGTGTGACTAAAATATCATTATTATAGTAGGAGTAAGGACTAATTATTATAGGATAGTTGATATCAATGTTATGAAAATTGAATCAGATACTGAACCGGCGAGACTTTTGGGTCATAGTTTAATTGATCCAATCTGTAAATAAATAATAAATAAAGTAAAATAAAGAAATAAATAAATGTATAGAATGAAAGTTGGTTGAACCGACCCGGTTGAACTGGTTCAACTATAGTTCAATCAGGTTCAACCAGACTACAATGTAACTAAAATCACACAAATTGACCGGTTGTCGATTCAACCTATTAAAAATGTGATCAATACTCTAGATATTTAAAAGTGATAAATTATTTGAGACAAAAACATGTTACAAATATAACAAACAATATTATATGAAGGAAGTACTATATGTTATATAGATTTTCATGTTGTTTTTTGTGTTAATTTTGGCTCTTTTCACTTCCTTCTTTAACATTAAGGTATGAAAAATATGAAACAGGTTGGTTGCAAATGGGAAAAGGTTAGTGAGACTAAATCACATAATGGAAGATGTGGAAAAAACCATTGAAGATAAAAATGAGAGGAAAAAGCTTTTGGAGGGCTTATTCGGATACATCCTAAGTTGTACACAGGAGGCAGCTATTGTTCCACCATATGTTGTTTTTGCTGTTAGGCCTAATCCAGGTTTCTGGGAATATGTTAAAGTGAATGCAGATGATTTGGAAGTAGATGGTATTGAGCCTTCAGAATACTTGAAATACAAGGAAATGGTATTTGATGAGAAGTGGTAAGTTGTTTTCTTAATTTGAAACTCATTAAGCATACAATTTTCTTTGGAGTAAATAAGCATTTTAGTCCTCGAAATTGTAAACATAGGGCAATTTCGTCCCTCAGATTTACAAAACAAATTAGTCCTACGTCAATATCAAAATATCTCATTAATAAACATCTATCAAAATCAATGAAAGACTTACATATCAAACTTGAATAGTCTCACGCTGCATAAAAAATAAGTATAAAATTTTCAAAACTGTACGGTTAAATTTGAAAAAAATACTAAATTGATTGACGTTCAAATTAAGGGATTAAATTGCTATTTTGCAAATTTCAAACTAAATTGACTGATCCTTATAATTTGAAGGACTAAAATGCTAATGTACTCATTTACTACGTGTCGATATTTATTTGACTCTTTGATAAGGCAAATGGTCCATGAAATGTTAGTTCCATGGCAAGAGCGTGACCGTAAGGGCCGAAGTTCAAATTTCTCTGGAGACAATTATAAAATGACTATTAGTGCTACTTTATTATCCCTTACTATTTTTTTTTCATTATAAAAAATGGTTAACATGAGTTGAATTTGCAGGGCAAATGATGAAAATGCTTTCGAGATAGATTTTGAGGCTATTGATTTCACCACACCTCATATGGCACTTTCATCTTCTATTGGAAAAGGACTTGACTTTACTACTAGGATCTTGACCTCAAGATTGAGTGAGAGTTCACATTGTGAAAATCCATTGCTTAATTACTTATTAAGCCTTAATCATCAAGGAGAGGTAATTAAAGGCATTAGTGTATCAAGTATTGAAATAAATGTGTACAATTGAAAAAAAAAATTAAAGTTAATTATGAAAATAAAAATTCTATATTTTTTTTTTTACCATATTGCAGAATCTTATAATCAAAGACACTTTGAATACAATACCAAAGCTTCAGAAAGCATTGAATCTAGCAGAAGCATATGTATCTGCACATCACAAAGACACACCATACCAGAATTTTGAAAACAGGTACGGACACTAGACATGACACTGTCACGTCCACCACTGACAATAATCTGAAAAAATGAAACAATTGAACGTTACCAAATGTTTTGGTGGCGTGTCACTGTGCGGACACACATTTAATCCGAAGTGTCAACATACTATTAATATATAATAATACTCAGTATTATGATTTTTGTTTTTTGTGAGCAGGTTTAGAGAATGGGGATTTGATAAGGGGTGGGGAAACACTGCAGGAAGGGTAAAAGAGACAATGAGAATGCTTTCTGAGGTACTAGAAGCAGGAGATCCAATTAAAATGGAATCACTTTTCAGCAGGCTACCAAACATGTTCAACATTGTTATTTTCTCTATTCATGGATACTTTGGTCAAGCTGATGTTCTAGGATTGCCAGACACTGGAGGCCAGGTTTTAATCCTTTCTTTTCACTGATTGAATCCATGAAAATTACTAGAAAATCAAATATTTTGTTGCCTAATTTTTGACACAATGAGTTCGCTTGCTTAACAATTAACCTTCAATATTAGGGAAATTTTATGACGTACCATCTCGACACTATATAACATTGGAAGTTTGAAAAACTGAATAATTTTTTCTTTATTGGGACTAACAGGTGGTATACATTCTTGATCAAGTAAGAGCACTAGAGGAAGAATTACTCCAAAAGATTGAATTACAAGGCCTTAATGTGAAGCCTCAGATTCTTGTGGTATGCTACCAAGTAGTAATACAAAAAATTACAAATAATGTATACCAATTTTTTATTTTTTTTTACAAATTATTATACTTAACAAAAAATTCGAAAAAGTATAAACCATCGTATTAATCTTCAATTTTATTTTGTTTCCTTTATATGTTTCATCGGCGTGCTGATGAATACTAATGTATTTGTATGTTCGGTTTTTTTCTTGCGCTAACGTGTGCCATGCACAGGTTACACGTTTGATACCGAATGCAAAAGAGACAACATGTAACCAAGAACTTGAACCTATCATCAAAACTAAGCATTCACACATTCTTAGAGTCCCTTTCTGGACAGAGAAGGGAATTCTATCCCAATGGGTGTCGCGTTTTGATATCTATCCTTATTTAGAAAGATTTGCTCAGGCATGTTGAATCATATCTTCATTTGATTTCCCTCTCTTCCATATTTGATATTACTATCAAGATTATGCATTTTAGTTCCTCTTTCTAATTATGCATTTTTATTATAAACAGGATTCTACTACCAAGATTCTTGAACTTATGGATGGAAAACCTGACCTCATTATTGGAAATTACACTGATGGAAACTTAGTTTCATCCTTAATGGCTTCTAAACTTGGTGTAACTCAGGTTAGCTTATGTGCTTATGGATAAACACCTTATTTAAGTGCTTATATCTACAAGTGCTTATATACAAGATATTTTTATAATAAAAGATAAAATAAAGTCAAAATATTTCTATATAAGTTATAATCTATTTTCAAAGCTATTCCGGAGAGCTTATATGTAAACAACTGAAAACAGCTTAAGACACATCATAAGCTGTTTCCATAAACTCTCCGAAACAGTCTCACAAATGCTCACGTGAGTAGATAAACTCAAATAAGTCAATCAAGAAGTATTCATTGGTATAAATATACATTCTTTTCCGATTACTCTATTAGTACTTTTGAACTAAAACTTGTATCCATAATGATTGAAGGCAACCATTGCTCATGCTTTGGAGAAGACAAAATATGAAGATTCAGATGCTAAATGGAATAGTTTTGAAGAAAAGTATCACTTTTCAAGTCAGTTCACAGCTGATCTAATCGCAATGAATTCCGCTGATTTTATCATAACTAGTACATACCAAGAAATTGCTGGAAGGTTGACATTTTTTCTTCTTCTTATAAATTATACATAAATTCTTATATGCTATCTCATGGTTAACGTAATATTATCATGAAGGTAACATTTTCATTGTTTTTTGTATCATCAGCAAGGATAGGCCAGGGCAATATGAAACTCACACTGCATTTACCATGCCGGGACTTTGTCGCGTAGTCTCCGGCATCAATGTATTTGATCCAAAGTTCAACATTGCTGCTCCAGGAGCTGATCAATCTGTCTACTTCCCTTTCACCGAGAAGAAGCAAAGATTGACCACTTTTCAACCTGCTATTGAAGAATTACTTTATAGTAAGGTTGAAAATGAAGAACACATGTGAGTTTCCTCCTATTACTTTCTCATCAAGTTATCATCCACTTGCATAGTATAGTAATGAGAATATAAAATAGTTAAAGGATTAGGGTTTAAATTCTAGCGTAGGAGAAAATACCGACATAACAGTTAATATACTAACATTTTTATAATTGCTTTCTTAAACATTATTGACATGTTTTGATGTTATGCTTAGCTTTATAAAAATGTACTTAACAATTGATACACAAGATGTATGTCTAAGGTGATTTACGGTTAAACGTCGTTGCTTCAATAAATCGTAACTAACTAAATTGTTTGCTCGAATTAAAATGTGTCTTGGAACAGTGGATTTTTGGAAGACAAGAAGAAACCTATAATCTTCTCAATGGCAAGGCTTGACAAAGTGAAAAACATTGGAGGCCTAGTCGAGTGGTTCGCGAAGAACAAGCAACTAAGAAGTTTAGTAAATCTTGTAATTGTTGGAGGATTCTTCGATCCTTCAAAATCCAAAGATAGAGAAGAAACAGAAGAAATCAAGAAAATGCATTACTTGATGAAAGAGTACAAACTGCAAGGTCAGTTCAGATGGATTGCGGCGCAAACTGATAGATATCGCAACGGAGAGCTTTACCGATGCATCGCGGATACAAAAGGAGCATTTGTTCAACCAGCATTGTATGAAGCTTTTGGTCTAACAGTGATTGAGGCAATGAACTGTGGATTACCAACTTTTGCAACAAACCAAGGTGGTCCAGCTGAAATTATAATTGATGGTGTCTCAGGTTTTCATATTGATCCTCATAATGGAGATGAATCAATCAACAAGGTTTCTGAATTTTTTGAGAAGTGCAAGACTAATCCTGAACATTGGAACATAGTTTCTAAAGCTGGTCTTGAAAGAATCAATGAATGGTACATTTCTGATTTATGGTTATGGATATTATCGGCAAAACAATAGTTAATATCGTATTAAACTTTGAAAATGACAAATAAATCGGAAAACTTTTTTTTTTATTTACGAAAGTGACAGTTAAAAAGGAACAGAGGGAGTAACGGACATGCGTAGTTTAACAAACATTCATATGGTTGGATTTCTTATCTTTATTTCTTGTGTTTTCTTTGTAGCTATACATGGAAGATATATGCAAACAAAGTGTTGAATATGGGATCAATTTATGGGTTTTGGAGAAAGTTGAACAAAGAACAAAAGTTGGCAAAGGAAAGATACATACAAATGTTTTATAATCTTCAATTTAGGAACTTGGTAAGTTTGATGTTATTTTTAATAGTTTTCATCGGTTTGTTACAAATTTTAAAAAAATGTAAATATAAAAAAAAAGAAATTAAAAACTAATTCAAATGAGAATCTGTTTTGAGTCGTTTCTCTGGAAAATAATTTTTTTATAAATGTTTTTCTTTTAAAAGATCGATTTTTATTTTGATAAATGAACACAAAATTGCTTCCGGTATTCTTGAAATCTCTAAAAACTAATCTCGGTATTATCGAATATAAAAATCACATTTTTACAATATATAACAAATAGGCCTTCTATGTCGCATGTATATGTCAGCCTCACTTTCGGATTTTCTCGTTTTTGGGATTTCTTTTGTGGTTTGTTTCTTTGTTTAGGGTGGTTTTTTAAAGGGGTTTTTGTTCGAGCTGGAGTTTGTATGGCAACCGATTTCCCCTTCTATGTATTTTTTTCTCCACCCGAACTTATTAGTGCGCCTTGCACGGACTTGTTGGTTAATAATATTTTGCCGATTCTAAAAAAAAAAAAACAAATAGGCCTTAAATATAATAAGAATTGAAAAGTTATGGGAAAATTTCATTGCTTTGCAGGCAAGAAAGGTACCAATTCCTAAGGTACCCCAAGAACCTCAACCAATGTCAAGAACTCCTGCCAAAAAAGCAGAAGCTACTTCCAATGGGCATGATGCAGCACAAGCAAAAGCTGAAGTACCTCAAACCCAGTTGGCATCCTTACCACCCAAAATTGAATCTAAGCTAACAACAAGGTAAGTCATTTATGCAAATAGCTATACTTCATGTGTCAAAATAAAAAAATGAGCAACAATTTCCTCAAATGAATATTTTGGTTTTAATTCAGCTTTGCATATGTAAAAGAAAAATGACTAAAGTACACTTTTCACCCCTAAATATTATATTATGATTGTGCATTTTCGGACTCAAGGTCTAGTTATGCAAATTGATTCTCACAAGTATCATTATTTTTACAATTAAGTTCATCATTCACCGAACATTCCAATTTTTAACGTCACTAGCCTAAACGTCCAATGTGGCGAACGAGTGTTGCATATGACGACTCATATTTTGTTACTTGTCGCATGTCAATATTCTATAAGCTAGTTTGAGTCAGTGACAACTAAGATCCATGTGTGTCATGTACGCTATTGAAGTTAAAATTCAACGACCTAGTTAGTGATCGAGGGACATGATTGTCACAAGATGGTTACTTAGAAACTTTGTTCGCACAATTAAAACTTTGAGACCGAAATTGCACAATCGTTATAATTATGCCATAGGGACCATGACAAAAAAATTAACATGGAAGAAATTTGCATGCAAAGTTAATAAATATAAAAATATATTTGATATGATTTAAATAAGAGATAAAACTTTATGAATCTTTTGCAGGGGTGAATCTTCAAGTAAGGAACTAGCTGCTAAACAAAGCGGTGATCTTTATTCTGGATTACGTTGGTTGCTTCCAGGAATTGCTTTTATGTTCATCATTCATTATCTCTCAAAGTATTTGGAACATTTATTCACAAGGGAGCAATAA
Protein sequence

>MS.gene05150.t1

MASTSSSALKRSDSIADSMPDALKQSRFHMKKCFARLVANGKRLVRLNHIMEDVEKTIEDKNERKKLLEGLFGYILSCTQEAAIVPPYVVFAVRPNPGFWEYVKVNADDLEVDGIEPSEYLKYKEMVFDEKWANDENAFEIDFEAIDFTTPHMALSSSIGKGLDFTTRILTSRLSESSHCENPLLNYLLSLNHQGENLIIKDTLNTIPKLQKALNLAEAYVSAHHKDTPYQNFENRFREWGFDKGWGNTAGRVKETMRMLSEVLEAGDPIKMESLFSRLPNMFNIVIFSIHGYFGQADVLGLPDTGGQVVYILDQVRALEEELLQKIELQGLNVKPQILVVTRLIPNAKETTCNQELEPIIKTKHSHILRVPFWTEKGILSQWVSRFDIYPYLERFAQDSTTKILELMDGKPDLIIGNYTDGNLVSSLMASKLGVTQATIAHALEKTKYEDSDAKWNSFEEKYHFSSQFTADLIAMNSADFIITSTYQEIAGSKDRPGQYETHTAFTMPGLCRVVSGINVFDPKFNIAAPGADQSVYFPFTEKKQRLTTFQPAIEELLYSKVENEEHIGFLEDKKKPIIFSMARLDKVKNIGGLVEWFAKNKQLRSLVNLVIVGGFFDPSKSKDREETEEIKKMHYLMKEYKLQGQFRWIAAQTDRYRNGELYRCIADTKGAFVQPALYEAFGLTVIEAMNCGLPTFATNQGGPAEIIIDGVSGFHIDPHNGDESINKVSEFFEKCKTNPEHWNIVSKAGLERINECYTWKIYANKVLNMGSIYGFWRKLNKEQKLAKERYIQMFYNLQFRNLARKVPIPKVPQEPQPMSRTPAKKAEATSNGHDAAQAKAEVPQTQLASLPPKIESKLTTRGESSSKELAAKQSGDLYSGLRWLLPGIAFMFIIHYLSKYLEHLFTREQ