Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene43005.t1 | GAU48083.1 | 77.9 | 149 | 29 | 2 | 10 | 158 | 124 | 268 | 1.00E-51 | 213 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene43005.t1 | A0A2Z6P0T0 | 77.9 | 149 | 29 | 2 | 10 | 158 | 124 | 268 | 7.2e-52 | 213.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050787 | MS.gene43005 | 0.819863 | 9.29E-53 | -1.69E-46 |
| MS.gene056875 | MS.gene43005 | 0.803258 | 3.80E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 35 sgRNAs with CRISPR-Local
Find 46 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGTATAATTTGAATATTTAT+TGG | 0.149686 | 8.1:+55365304 | None:intergenic |
| AAATATAGATTTGGATTTAA+TGG | 0.180402 | 8.1:-55365153 | MS.gene43005:CDS |
| AATGCAAAGGGTAGAGTATA+TGG | 0.262241 | 8.1:-55365374 | MS.gene43005:CDS |
| GAGAGGTTGAGGCGCGTTTA+AGG | 0.273052 | 8.1:-55365259 | MS.gene43005:CDS |
| ATTTGCATGTTTAGCAAATA+CGG | 0.348024 | 8.1:+55365172 | None:intergenic |
| TAAACATGCAAATATAGATT+TGG | 0.354681 | 8.1:-55365162 | MS.gene43005:CDS |
| ACAAACAATCGATTCCATTA+AGG | 0.358541 | 8.1:-55365216 | MS.gene43005:CDS |
| TAGAGTATATGGGCTTGGTT+TGG | 0.416967 | 8.1:-55365363 | MS.gene43005:CDS |
| ATTAGTGTATCATAATCAAT+AGG | 0.448901 | 8.1:+55365419 | None:intergenic |
| TGAGTCTCAACCTCATCCTT+TGG | 0.456749 | 8.1:-55365477 | MS.gene43005:CDS |
| GTCTCAACCTCATCCTTTGG+AGG | 0.479026 | 8.1:-55365474 | MS.gene43005:CDS |
| GGATTTAGACGTTGAGCCAC+GGG | 0.479351 | 8.1:+55365325 | None:intergenic |
| CAACCTCATCCTTTGGAGGC+TGG | 0.487170 | 8.1:-55365470 | MS.gene43005:CDS |
| AAGGGTAGAGTATATGGGCT+TGG | 0.493623 | 8.1:-55365368 | MS.gene43005:CDS |
| TGTATCATAATCAATAGGCT+TGG | 0.504722 | 8.1:+55365424 | None:intergenic |
| GTATCATAATCAATAGGCTT+GGG | 0.509867 | 8.1:+55365425 | None:intergenic |
| TGGTATGAGTGGAGAGGTTG+AGG | 0.514731 | 8.1:-55365270 | MS.gene43005:CDS |
| GTATATGGGCTTGGTTTGGA+TGG | 0.514998 | 8.1:-55365359 | MS.gene43005:CDS |
| ATGCAAAGGGTAGAGTATAT+GGG | 0.526831 | 8.1:-55365373 | MS.gene43005:CDS |
| ACACTAATGATTGAAGCTGT+TGG | 0.537396 | 8.1:-55365404 | MS.gene43005:CDS |
| GATGGCAGCATGCGCACCCG+TGG | 0.550034 | 8.1:-55365341 | MS.gene43005:CDS |
| CTAATGATTGAAGCTGTTGG+AGG | 0.555236 | 8.1:-55365401 | MS.gene43005:CDS |
| TGCAAATGTTGGTGCTGATG+TGG | 0.559498 | 8.1:-55365081 | MS.gene43005:CDS |
| TGGATTTAGACGTTGAGCCA+CGG | 0.559998 | 8.1:+55365324 | None:intergenic |
| TTACCAGCCTCCAAAGGATG+AGG | 0.600248 | 8.1:+55365467 | None:intergenic |
| CGTGGAAGCAATGCAAATGT+TGG | 0.616233 | 8.1:-55365092 | MS.gene43005:CDS |
| TCATCATTACCAGCCTCCAA+AGG | 0.621367 | 8.1:+55365461 | None:intergenic |
| TGTTGGAGGAAGAAATGCAA+AGG | 0.632509 | 8.1:-55365387 | MS.gene43005:CDS |
| GATATTGCACGTGGTATGAG+TGG | 0.632896 | 8.1:-55365281 | MS.gene43005:CDS |
| TCTGTTGATAATGCTACTCG+TGG | 0.635589 | 8.1:-55365110 | MS.gene43005:CDS |
| GTTGGAGGAAGAAATGCAAA+GGG | 0.639504 | 8.1:-55365386 | MS.gene43005:CDS |
| AATTATACTGATATTGCACG+TGG | 0.677934 | 8.1:-55365290 | MS.gene43005:CDS |
| TATGAATCTAGTAATGTCTG+AGG | 0.710672 | 8.1:-55365707 | MS.gene43005:intron |
| TGCACGTGGTATGAGTGGAG+AGG | 0.729243 | 8.1:-55365276 | MS.gene43005:CDS |
| GAGGCTGGTAATGATGAGAG+TGG | 0.750491 | 8.1:-55365455 | MS.gene43005:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AGTATAATTTGAATATTTAT+TGG | + | chr8.1:55365478-55365497 | None:intergenic | 10.0% |
| !! | AAATATAGATTTGGATTTAA+TGG | - | chr8.1:55365626-55365645 | MS.gene43005:intron | 15.0% |
| !! | AATAACTGAATAAATAAGAT+AGG | + | chr8.1:55365243-55365262 | None:intergenic | 15.0% |
| !! | ATTAGTGTATCATAATCAAT+AGG | + | chr8.1:55365363-55365382 | None:intergenic | 20.0% |
| !! | TAAACATGCAAATATAGATT+TGG | - | chr8.1:55365617-55365636 | MS.gene43005:intron | 20.0% |
| ! | ATTTGCATGTTTAGCAAATA+CGG | + | chr8.1:55365610-55365629 | None:intergenic | 25.0% |
| ! | CATGTATTGCCTTTATAATT+TGG | - | chr8.1:55365140-55365159 | MS.gene43005:CDS | 25.0% |
| !!! | CCTTTATAATTTGGTTTGTT+GGG | - | chr8.1:55365149-55365168 | MS.gene43005:CDS | 25.0% |
| AATTATACTGATATTGCACG+TGG | - | chr8.1:55365489-55365508 | MS.gene43005:CDS | 30.0% | |
| CAGTTTAAGAAGGCAAATTA+AGG | + | chr8.1:55365207-55365226 | None:intergenic | 30.0% | |
| CCCAACAAACCAAATTATAA+AGG | + | chr8.1:55365152-55365171 | None:intergenic | 30.0% | |
| GTATCATAATCAATAGGCTT+GGG | + | chr8.1:55365357-55365376 | None:intergenic | 30.0% | |
| TGTATCATAATCAATAGGCT+TGG | + | chr8.1:55365358-55365377 | None:intergenic | 30.0% | |
| TTAAGAAGGCAAATTAAGGA+GGG | + | chr8.1:55365203-55365222 | None:intergenic | 30.0% | |
| TTTAAGAAGGCAAATTAAGG+AGG | + | chr8.1:55365204-55365223 | None:intergenic | 30.0% | |
| TTTGTTGGGATGTTCTATTT+AGG | - | chr8.1:55365163-55365182 | MS.gene43005:CDS | 30.0% | |
| ! | ACAAACAATCGATTCCATTA+AGG | - | chr8.1:55365563-55365582 | MS.gene43005:intron | 30.0% |
| !! | GCCTTTATAATTTGGTTTGT+TGG | - | chr8.1:55365148-55365167 | MS.gene43005:CDS | 30.0% |
| AATGCAAAGGGTAGAGTATA+TGG | - | chr8.1:55365405-55365424 | MS.gene43005:CDS | 35.0% | |
| ACAAGCAACACAGTTTAAGA+AGG | + | chr8.1:55365217-55365236 | None:intergenic | 35.0% | |
| ACACTAATGATTGAAGCTGT+TGG | - | chr8.1:55365375-55365394 | MS.gene43005:CDS | 35.0% | |
| ATGCAAAGGGTAGAGTATAT+GGG | - | chr8.1:55365406-55365425 | MS.gene43005:CDS | 35.0% | |
| !! | GTAATCGAGTGACTTGTTAT+AGG | - | chr8.1:55365114-55365133 | MS.gene43005:CDS | 35.0% |
| !!! | TTTGTTTTGACGAGCCTTAA+TGG | + | chr8.1:55365580-55365599 | None:intergenic | 35.0% |
| CTAATGATTGAAGCTGTTGG+AGG | - | chr8.1:55365378-55365397 | MS.gene43005:CDS | 40.0% | |
| GTTGGAGGAAGAAATGCAAA+GGG | - | chr8.1:55365393-55365412 | MS.gene43005:CDS | 40.0% | |
| TGTTGGAGGAAGAAATGCAA+AGG | - | chr8.1:55365392-55365411 | MS.gene43005:CDS | 40.0% | |
| ! | TAGAGTATATGGGCTTGGTT+TGG | - | chr8.1:55365416-55365435 | MS.gene43005:CDS | 40.0% |
| !! | TCTGTTGATAATGCTACTCG+TGG | - | chr8.1:55365669-55365688 | MS.gene43005:intron | 40.0% |
| AAGGGTAGAGTATATGGGCT+TGG | - | chr8.1:55365411-55365430 | MS.gene43005:CDS | 45.0% | |
| CGTGGAAGCAATGCAAATGT+TGG | - | chr8.1:55365687-55365706 | MS.gene43005:intron | 45.0% | |
| GATATTGCACGTGGTATGAG+TGG | - | chr8.1:55365498-55365517 | MS.gene43005:CDS | 45.0% | |
| TCATCATTACCAGCCTCCAA+AGG | + | chr8.1:55365321-55365340 | None:intergenic | 45.0% | |
| TGAGTCTCAACCTCATCCTT+TGG | - | chr8.1:55365302-55365321 | MS.gene43005:CDS | 45.0% | |
| TGGATTTAGACGTTGAGCCA+CGG | + | chr8.1:55365458-55365477 | None:intergenic | 45.0% | |
| !! | GTATATGGGCTTGGTTTGGA+TGG | - | chr8.1:55365420-55365439 | MS.gene43005:CDS | 45.0% |
| !! | TGCAAATGTTGGTGCTGATG+TGG | - | chr8.1:55365698-55365717 | MS.gene43005:intron | 45.0% |
| GGATTTAGACGTTGAGCCAC+GGG | + | chr8.1:55365457-55365476 | None:intergenic | 50.0% | |
| GTCTCAACCTCATCCTTTGG+AGG | - | chr8.1:55365305-55365324 | MS.gene43005:CDS | 50.0% | |
| TGGTATGAGTGGAGAGGTTG+AGG | - | chr8.1:55365509-55365528 | MS.gene43005:CDS | 50.0% | |
| TTACCAGCCTCCAAAGGATG+AGG | + | chr8.1:55365315-55365334 | None:intergenic | 50.0% | |
| ! | GAGGCTGGTAATGATGAGAG+TGG | - | chr8.1:55365324-55365343 | MS.gene43005:CDS | 50.0% |
| GAGAGGTTGAGGCGCGTTTA+AGG | - | chr8.1:55365520-55365539 | MS.gene43005:intron | 55.0% | |
| TGCACGTGGTATGAGTGGAG+AGG | - | chr8.1:55365503-55365522 | MS.gene43005:CDS | 55.0% | |
| ! | CAACCTCATCCTTTGGAGGC+TGG | - | chr8.1:55365309-55365328 | MS.gene43005:CDS | 55.0% |
| GATGGCAGCATGCGCACCCG+TGG | - | chr8.1:55365438-55365457 | MS.gene43005:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.1 | gene | 55365073 | 55365728 | 55365073 | ID=MS.gene43005 |
| chr8.1 | mRNA | 55365073 | 55365728 | 55365073 | ID=MS.gene43005.t1;Parent=MS.gene43005 |
| chr8.1 | exon | 55365708 | 55365728 | 55365708 | ID=MS.gene43005.t1.exon1;Parent=MS.gene43005.t1 |
| chr8.1 | CDS | 55365708 | 55365728 | 55365708 | ID=cds.MS.gene43005.t1;Parent=MS.gene43005.t1 |
| chr8.1 | exon | 55365073 | 55365528 | 55365073 | ID=MS.gene43005.t1.exon2;Parent=MS.gene43005.t1 |
| chr8.1 | CDS | 55365073 | 55365528 | 55365073 | ID=cds.MS.gene43005.t1;Parent=MS.gene43005.t1 |
| Gene Sequence |
| Protein sequence |
