AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene43083

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene43083.t1 MTR_2g084875 98.592 426 6 0 1 426 1 426 0.0 873
MS.gene43083.t1 MTR_4g055310 80.751 426 65 6 1 424 1 411 0.0 681
MS.gene43083.t1 MTR_4g132250 67.105 304 98 1 116 419 89 390 4.58e-150 431
MS.gene43083.t1 MTR_2g088130 65.033 306 105 1 116 421 71 374 1.36e-148 427
MS.gene43083.t1 MTR_4g061070 66.013 306 99 2 116 421 73 373 4.10e-141 408
MS.gene43083.t1 MTR_2g088130 67.029 276 89 1 146 421 51 324 3.22e-135 391
MS.gene43083.t1 MTR_2g088130 64.504 262 91 1 116 377 71 330 1.52e-123 362
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene43083.t1 AT1G08250 81.250 384 53 5 49 420 26 402 0.0 613
MS.gene43083.t1 AT2G27820 82.289 367 58 3 58 421 46 408 0.0 591
MS.gene43083.t1 AT3G44720 72.886 402 88 5 32 424 28 417 0.0 574
MS.gene43083.t1 AT5G22630 70.602 432 107 4 1 425 1 419 0.0 564
MS.gene43083.t1 AT1G11790 63.072 306 111 1 116 421 88 391 3.99e-145 419
MS.gene43083.t1 AT3G07630 66.990 309 95 2 113 421 78 379 1.50e-139 404
MS.gene43083.t1 AT3G07630 66.990 309 95 2 113 421 78 379 1.50e-139 404
MS.gene43083.t1 AT3G07630 66.990 309 95 2 113 421 78 379 1.50e-139 404
MS.gene43083.t1 AT1G11790 61.728 243 91 1 116 358 88 328 6.04e-108 322

Find 121 sgRNAs with CRISPR-Local

Find 142 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCCCGTGCTGCCGAGCTTTA+TGG 0.134872 2.4:+14347955 MS.gene43083:CDS
CGATCCGTACGTGGAATTAT+TGG 0.235123 2.4:-14348051 None:intergenic
CACGTCTTTCTTGAGAGTTT+TGG 0.267407 2.4:+14348329 MS.gene43083:CDS
TCGGAGATTGTTAACGGTTT+TGG 0.276157 2.4:-14347457 None:intergenic
CGTTTCAAGCCGTCGAGCTT+TGG 0.306242 2.4:+14347614 MS.gene43083:CDS
CAAGGAATTGCTTCGCAGTT+TGG 0.320220 2.4:-14347574 None:intergenic
ATGAGCTTTGCCAATCGGTT+CGG 0.347567 2.4:-14347261 None:intergenic
AAACGCGTTGGCTGAAGTTC+AGG 0.354971 2.4:+14348302 MS.gene43083:CDS
TGAGCTTTGCCAATCGGTTC+GGG 0.367455 2.4:-14347260 None:intergenic
ACGAATCGGGTCACGTTACT+CGG 0.372580 2.4:-14348012 None:intergenic
TACGGTTCAATTTATGAATC+CGG 0.377562 2.4:+14347232 MS.gene43083:CDS
CGGTTCGGACCGAGCCGGTT+TGG 0.384226 2.4:-14347190 None:intergenic
TTGTTATGTGGTTGAAATTG+AGG 0.385367 2.4:-14347147 None:intergenic
TACGTGGAATTATTGGTTCT+CGG 0.399885 2.4:-14348044 None:intergenic
CGCCGACCGTGCCGTTCTCC+CGG 0.401840 2.4:+14347639 MS.gene43083:CDS
TTCATCGGCGCCGGTGTTGA+CGG 0.402723 2.4:-14347338 None:intergenic
CCAATCGGTTCGGGTTGAAC+CGG 0.407367 2.4:-14347251 None:intergenic
CTTGAAACGCCACTTCGAAT+TGG 0.410792 2.4:-14347600 None:intergenic
CAAACCGGCTCGGTCCGAAC+CGG 0.410873 2.4:+14347191 MS.gene43083:CDS
GGTGTTGACGGCGGTGGAAG+TGG 0.419833 2.4:-14347326 None:intergenic
CGTGCATCGGTGCCGGAGCT+AGG 0.422738 2.4:-14347480 None:intergenic
AAGCTCGGCAGCACGGGCGC+TGG 0.431811 2.4:-14347950 None:intergenic
GCATCATCAACAAGCCTAAT+CGG 0.435985 2.4:-14348201 None:intergenic
TCCATAAAGCTCGGCAGCAC+GGG 0.437399 2.4:-14347956 None:intergenic
GCGGCTTCGGAGTATGCTCC+CGG 0.439700 2.4:-14347535 None:intergenic
GTTTCTGTCAAATGTGCTTA+CGG 0.450930 2.4:+14347214 MS.gene43083:CDS
CTTGAGCCGTGCATCGGTGC+CGG 0.455252 2.4:-14347487 None:intergenic
GCGAGGAGACAGTGATGGAC+CGG 0.457534 2.4:-14347748 None:intergenic
AACAATCTCCGACCTAGCTC+CGG 0.461711 2.4:+14347468 MS.gene43083:CDS
TGTTATGTGGTTGAAATTGA+GGG 0.461933 2.4:-14347146 None:intergenic
AGCACATGAGCTTTGCCAAT+CGG 0.463772 2.4:-14347266 None:intergenic
AGGGTGTTGGAAGAGGGAGG+TGG 0.465964 2.4:-14347127 None:intergenic
GGCGCCGGTGTTGACGGCGG+TGG 0.466117 2.4:-14347332 None:intergenic
ACGTGGAATTATTGGTTCTC+GGG 0.468317 2.4:-14348043 None:intergenic
CACTGTCTCCTCGCTCTCCC+CGG 0.469363 2.4:+14347757 MS.gene43083:CDS
GAAATTGAGGGTGTTGGAAG+AGG 0.470220 2.4:-14347134 None:intergenic
CTCGGGCCAACATAACGAAT+CGG 0.470637 2.4:-14348026 None:intergenic
AGTATGCTCCCGGGACTCCT+TGG 0.480032 2.4:-14347525 None:intergenic
GTGGTTGAAATTGAGGGTGT+TGG 0.481711 2.4:-14347140 None:intergenic
GCTAGGTCGGAGATTGTTAA+CGG 0.485559 2.4:-14347463 None:intergenic
CGTCGCTTACCAAGGAGTCC+CGG 0.492434 2.4:+14347516 MS.gene43083:CDS
AAATTGAGGGTGTTGGAAGA+GGG 0.492645 2.4:-14347133 None:intergenic
CTCATCGTAACCGTCCGATT+AGG 0.493486 2.4:+14348187 MS.gene43083:CDS
GTTTAACGAAGATTGAATCA+AGG 0.498900 2.4:+14348163 MS.gene43083:CDS
GGCCGTGGAAATTTCCAAAC+CGG 0.499246 2.4:+14347176 MS.gene43083:CDS
CGGAGGCTTGAGCCGTGCAT+CGG 0.506028 2.4:-14347493 None:intergenic
CTTGTTGATGATGCAAATGT+TGG 0.508037 2.4:+14348210 MS.gene43083:CDS
AAGTGGCTTTGAAATTTCTC+CGG 0.511464 2.4:-14347431 None:intergenic
AAACCGGCTCGGTCCGAACC+GGG 0.515637 2.4:+14347192 MS.gene43083:CDS
GGAGGAGGAGGTCGTAGTTA+CGG 0.523218 2.4:-14347690 None:intergenic
TTCTCCGGTGAGGTTTCCGA+TGG 0.523780 2.4:-14347416 None:intergenic
CGAGGAGACAGTGATGGACC+GGG 0.526691 2.4:-14347747 None:intergenic
ACTTCCACCGCCGTCAACAC+CGG 0.528044 2.4:+14347328 MS.gene43083:CDS
CTTCTCCGACGATGTGGAGA+CGG 0.530142 2.4:-14347717 None:intergenic
GTCGCTTACCAAGGAGTCCC+GGG 0.532943 2.4:+14347517 MS.gene43083:CDS
CTCCCGGTGGAAAATTCCCT+CGG 0.533003 2.4:+14347655 MS.gene43083:CDS
ACATAACAACAACACGGCCG+TGG 0.534054 2.4:+14347161 MS.gene43083:CDS
TGGCGATAGCTGCAGTGTCG+CGG 0.534684 2.4:-14347930 None:intergenic
TGTGGAGACGGTGACGGAGG+AGG 0.537316 2.4:-14347705 None:intergenic
TTCTGATTCCGGGGAGAGCG+AGG 0.539157 2.4:-14347765 None:intergenic
TCGGGCCAACATAACGAATC+GGG 0.540780 2.4:-14348025 None:intergenic
CACCGGGAGAACGGCACGGT+CGG 0.542354 2.4:-14347641 None:intergenic
GCATACTCCGAAGCCGCCGC+CGG 0.543941 2.4:+14347541 MS.gene43083:CDS
GGATTAAATATCTTAGCAGA+TGG 0.548048 2.4:+14347976 MS.gene43083:CDS
AGCCGGTTTGGAAATTTCCA+CGG 0.549886 2.4:-14347178 None:intergenic
ATCCATAAAGCTCGGCAGCA+CGG 0.552752 2.4:-14347957 None:intergenic
GATATTTAATCCATAAAGCT+CGG 0.553451 2.4:-14347965 None:intergenic
CGAATCGGGTCACGTTACTC+GGG 0.554957 2.4:-14348011 None:intergenic
CGTGACCCGATTCGTTATGT+TGG 0.557359 2.4:+14348020 MS.gene43083:CDS
ACGGCCGTGTTGTTGTTATG+TGG 0.560779 2.4:-14347159 None:intergenic
GGAGACGGTGACGGAGGAGG+AGG 0.561047 2.4:-14347702 None:intergenic
TTGAGGGTGTTGGAAGAGGG+AGG 0.565084 2.4:-14347130 None:intergenic
CATCGGTGCCGGAGCTAGGT+CGG 0.567651 2.4:-14347476 None:intergenic
CTGCTTCACGTGCGACGTTG+AGG 0.571118 2.4:-14347861 None:intergenic
CCGGTTCAACCCGAACCGAT+TGG 0.571365 2.4:+14347251 MS.gene43083:CDS
TGGAAATTTCCAAACCGGCT+CGG 0.578350 2.4:+14347181 MS.gene43083:CDS
CATTTGACAGAAACCCGGTT+CGG 0.579637 2.4:-14347205 None:intergenic
GTCCAGGGTGTCATATCCAT+AGG 0.579769 2.4:-14348360 None:intergenic
GGTTTCCGATGGGAACAAGC+TGG 0.586510 2.4:-14347405 None:intergenic
GGAGAGCGAGGAGACAGTGA+TGG 0.588961 2.4:-14347753 None:intergenic
CTAGCTCCGGCACCGATGCA+CGG 0.590713 2.4:+14347481 MS.gene43083:CDS
GATGGGAACAAGCTGGAGAT+CGG 0.591431 2.4:-14347398 None:intergenic
AGCCTCCGCGTCGCTTACCA+AGG 0.596298 2.4:+14347508 MS.gene43083:CDS
GTTCCCATCGGAAACCTCAC+CGG 0.598886 2.4:+14347412 MS.gene43083:CDS
GCCACTTCGAATTGGTCGCA+AGG 0.603521 2.4:-14347592 None:intergenic
GAAAACTTTGCAGCTATCAA+CGG 0.603638 2.4:+14347358 MS.gene43083:CDS
GAACACACATTAACAAAACT+CGG 0.604317 2.4:+14347838 MS.gene43083:CDS
TCCAGGGTGTCATATCCATA+GGG 0.610493 2.4:-14348359 None:intergenic
TCGCATTGAGATAAAGCTTG+TGG 0.611630 2.4:-14347817 None:intergenic
GTTGAACTTCTCCGACGATG+TGG 0.613892 2.4:-14347723 None:intergenic
CGGCTTCGGAGTATGCTCCC+GGG 0.614890 2.4:-14347534 None:intergenic
ATCGGCGCCGGTGTTGACGG+CGG 0.615138 2.4:-14347335 None:intergenic
AGAACCAATAATTCCACGTA+CGG 0.615247 2.4:+14348047 MS.gene43083:CDS
GCTTTGAAATTTCTCCGGTG+AGG 0.615736 2.4:-14347426 None:intergenic
CGTCGGAGAAGTTCAACTCC+CGG 0.615992 2.4:+14347729 MS.gene43083:CDS
CGACGATGTGGAGACGGTGA+CGG 0.619755 2.4:-14347711 None:intergenic
AAACCCGGTTCGGACCGAGC+CGG 0.621805 2.4:-14347195 None:intergenic
TCCTTGCGACCAATTCGAAG+TGG 0.628248 2.4:+14347591 MS.gene43083:CDS
GATCTCCAGCTTGTTCCCAT+CGG 0.632237 2.4:+14347400 MS.gene43083:CDS
GAAGCAGTTGACGATACAGC+AGG 0.633337 2.4:+14347877 MS.gene43083:CDS
AGTTAGAGCACAAAACGCGT+TGG 0.634200 2.4:+14348290 MS.gene43083:CDS
GTCGGCGATCCAAAGCTCGA+CGG 0.635888 2.4:-14347623 None:intergenic
TTTCCACCGGGAGAACGGCA+CGG 0.637127 2.4:-14347645 None:intergenic
TACGGTGGATTGAACCGCCG+AGG 0.637797 2.4:-14347672 None:intergenic
TTAAATGGACGATCCGTACG+TGG 0.638943 2.4:-14348060 None:intergenic
TGACGATACAGCAGGTGCCG+CGG 0.640188 2.4:+14347885 MS.gene43083:CDS
CGTCACCGTCTCCACATCGT+CGG 0.640602 2.4:+14347712 MS.gene43083:CDS
TCTCCGGTGAGGTTTCCGAT+GGG 0.641911 2.4:-14347415 None:intergenic
CATCAACAAGCCTAATCGGA+CGG 0.642133 2.4:-14348197 None:intergenic
ACGGTGGATTGAACCGCCGA+GGG 0.644785 2.4:-14347671 None:intergenic
CGACCGTGCCGTTCTCCCGG+TGG 0.649026 2.4:+14347642 MS.gene43083:CDS
AAGCACATTTGACAGAAACC+CGG 0.662936 2.4:-14347210 None:intergenic
CTCCTTGGTAAGCGACGCGG+AGG 0.666074 2.4:-14347510 None:intergenic
CGATGTGGAGACGGTGACGG+AGG 0.671566 2.4:-14347708 None:intergenic
GGACTCCTTGGTAAGCGACG+CGG 0.679370 2.4:-14347513 None:intergenic
GGAGGAGGTCGTAGTTACGG+TGG 0.683087 2.4:-14347687 None:intergenic
CTAATCGGACGGTTACGATG+AGG 0.686334 2.4:-14348186 None:intergenic
ACCCTATGGATATGACACCC+TGG 0.690768 2.4:+14348358 MS.gene43083:CDS
TCAACCACATAACAACAACA+CGG 0.716010 2.4:+14347155 MS.gene43083:CDS
GTTAGTTGCAACAAACTCCG+CGG 0.733174 2.4:-14347902 None:intergenic
CCGGTGGAAAATTCCCTCGG+CGG 0.738047 2.4:+14347658 MS.gene43083:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAACAATACTTGTTTTAAA+TGG - chr2.4:14348078-14348097 None:intergenic 15.0%
! GATATTTAATCCATAAAGCT+CGG - chr2.4:14347968-14347987 None:intergenic 25.0%
GAACACACATTAACAAAACT+CGG + chr2.4:14347838-14347857 MS.gene43083:CDS 30.0%
GGATTAAATATCTTAGCAGA+TGG + chr2.4:14347976-14347995 MS.gene43083:CDS 30.0%
GTTTAACGAAGATTGAATCA+AGG + chr2.4:14348163-14348182 MS.gene43083:CDS 30.0%
TACGGTTCAATTTATGAATC+CGG + chr2.4:14347232-14347251 MS.gene43083:CDS 30.0%
TGTTATGTGGTTGAAATTGA+GGG - chr2.4:14347149-14347168 None:intergenic 30.0%
TTGTTATGTGGTTGAAATTG+AGG - chr2.4:14347150-14347169 None:intergenic 30.0%
! AGATATTCTTTTCTGATTCC+GGG - chr2.4:14347778-14347797 None:intergenic 30.0%
! ATTGTTTTCGCTCATGATAA+AGG + chr2.4:14348090-14348109 MS.gene43083:CDS 30.0%
! CAGATATTCTTTTCTGATTC+CGG - chr2.4:14347779-14347798 None:intergenic 30.0%
!!! TGTTGATTTTGAAGCTTCAA+TGG + chr2.4:14348263-14348282 MS.gene43083:CDS 30.0%
AGAACCAATAATTCCACGTA+CGG + chr2.4:14348047-14348066 MS.gene43083:CDS 35.0%
GAAAACTTTGCAGCTATCAA+CGG + chr2.4:14347358-14347377 MS.gene43083:CDS 35.0%
GTTTCTGTCAAATGTGCTTA+CGG + chr2.4:14347214-14347233 MS.gene43083:CDS 35.0%
TCAACCACATAACAACAACA+CGG + chr2.4:14347155-14347174 MS.gene43083:CDS 35.0%
! ACGTCTTTCTTGAGAGTTTT+GGG + chr2.4:14348330-14348349 MS.gene43083:CDS 35.0%
! GATAGCTGCAAAGTTTTCAT+CGG - chr2.4:14347356-14347375 None:intergenic 35.0%
! GATATTCTTTTCTGATTCCG+GGG - chr2.4:14347777-14347796 None:intergenic 35.0%
!! AAGTGGCTTTGAAATTTCTC+CGG - chr2.4:14347434-14347453 None:intergenic 35.0%
!! CTTGTTGATGATGCAAATGT+TGG + chr2.4:14348210-14348229 MS.gene43083:CDS 35.0%
!! TACGTGGAATTATTGGTTCT+CGG - chr2.4:14348047-14348066 None:intergenic 35.0%
!!! GATCGGTTATGTTTGTTTTG+TGG - chr2.4:14347384-14347403 None:intergenic 35.0%
AAGCACATTTGACAGAAACC+CGG - chr2.4:14347213-14347232 None:intergenic 40.0%
GCATCATCAACAAGCCTAAT+CGG - chr2.4:14348204-14348223 None:intergenic 40.0%
TCGCATTGAGATAAAGCTTG+TGG - chr2.4:14347820-14347839 None:intergenic 40.0%
! CACGTCTTTCTTGAGAGTTT+TGG + chr2.4:14348329-14348348 MS.gene43083:CDS 40.0%
! GCAGTTTGGATAAGCTTTTC+CGG - chr2.4:14347563-14347582 None:intergenic 40.0%
! TCGGAGATTGTTAACGGTTT+TGG - chr2.4:14347460-14347479 None:intergenic 40.0%
! TGCTTTCAGCTTTTGCGTTT+AGG + chr2.4:14348133-14348152 MS.gene43083:CDS 40.0%
!! AAATTGAGGGTGTTGGAAGA+GGG - chr2.4:14347136-14347155 None:intergenic 40.0%
!! ACGTGGAATTATTGGTTCTC+GGG - chr2.4:14348046-14348065 None:intergenic 40.0%
!! GAGATTGTTAACGGTTTTGG+TGG - chr2.4:14347457-14347476 None:intergenic 40.0%
!!! AGTTTTGGGTAGTTACCCTA+TGG + chr2.4:14348344-14348363 MS.gene43083:CDS 40.0%
AGCACATGAGCTTTGCCAAT+CGG - chr2.4:14347269-14347288 None:intergenic 45.0%
AGTTAGAGCACAAAACGCGT+TGG + chr2.4:14348290-14348309 MS.gene43083:CDS 45.0%
ATGAGCTTTGCCAATCGGTT+CGG - chr2.4:14347264-14347283 None:intergenic 45.0%
CAAGGAATTGCTTCGCAGTT+TGG - chr2.4:14347577-14347596 None:intergenic 45.0%
CATCAACAAGCCTAATCGGA+CGG - chr2.4:14348200-14348219 None:intergenic 45.0%
CATTTGACAGAAACCCGGTT+CGG - chr2.4:14347208-14347227 None:intergenic 45.0%
CGATCCGTACGTGGAATTAT+TGG - chr2.4:14348054-14348073 None:intergenic 45.0%
CTTGAAACGCCACTTCGAAT+TGG - chr2.4:14347603-14347622 None:intergenic 45.0%
GCTAGGTCGGAGATTGTTAA+CGG - chr2.4:14347466-14347485 None:intergenic 45.0%
GTTAGTTGCAACAAACTCCG+CGG - chr2.4:14347905-14347924 None:intergenic 45.0%
TGGAAATTTCCAAACCGGCT+CGG + chr2.4:14347181-14347200 MS.gene43083:CDS 45.0%
TTAAATGGACGATCCGTACG+TGG - chr2.4:14348063-14348082 None:intergenic 45.0%
! GTGGTTGAAATTGAGGGTGT+TGG - chr2.4:14347143-14347162 None:intergenic 45.0%
! GTTTGGATAAGCTTTTCCGG+CGG - chr2.4:14347560-14347579 None:intergenic 45.0%
! TCCAGGGTGTCATATCCATA+GGG - chr2.4:14348362-14348381 None:intergenic 45.0%
!! AGCCGGTTTGGAAATTTCCA+CGG - chr2.4:14347181-14347200 None:intergenic 45.0%
!! GAAATTGAGGGTGTTGGAAG+AGG - chr2.4:14347137-14347156 None:intergenic 45.0%
!! GCTTTGAAATTTCTCCGGTG+AGG - chr2.4:14347429-14347448 None:intergenic 45.0%
!! GTTAACGGTTTTGGTGGAAG+TGG - chr2.4:14347451-14347470 None:intergenic 45.0%
AACAATCTCCGACCTAGCTC+CGG + chr2.4:14347468-14347487 MS.gene43083:CDS 50.0%
ACATAACAACAACACGGCCG+TGG + chr2.4:14347161-14347180 MS.gene43083:CDS 50.0%
ACCCTATGGATATGACACCC+TGG + chr2.4:14348358-14348377 MS.gene43083:CDS 50.0%
ACGAATCGGGTCACGTTACT+CGG - chr2.4:14348015-14348034 None:intergenic 50.0%
ATCCATAAAGCTCGGCAGCA+CGG - chr2.4:14347960-14347979 None:intergenic 50.0%
CTAATCGGACGGTTACGATG+AGG - chr2.4:14348189-14348208 None:intergenic 50.0%
CTCATCGTAACCGTCCGATT+AGG + chr2.4:14348187-14348206 MS.gene43083:CDS 50.0%
CTCGGGCCAACATAACGAAT+CGG - chr2.4:14348029-14348048 None:intergenic 50.0%
GAAGCAGTTGACGATACAGC+AGG + chr2.4:14347877-14347896 MS.gene43083:CDS 50.0%
GATGGGAACAAGCTGGAGAT+CGG - chr2.4:14347401-14347420 None:intergenic 50.0%
GGCCGTGGAAATTTCCAAAC+CGG + chr2.4:14347176-14347195 MS.gene43083:CDS 50.0%
GTTGAACTTCTCCGACGATG+TGG - chr2.4:14347726-14347745 None:intergenic 50.0%
TCCTTGCGACCAATTCGAAG+TGG + chr2.4:14347591-14347610 MS.gene43083:CDS 50.0%
TCGGGCCAACATAACGAATC+GGG - chr2.4:14348028-14348047 None:intergenic 50.0%
! ACGGCCGTGTTGTTGTTATG+TGG - chr2.4:14347162-14347181 None:intergenic 50.0%
! CGTGACCCGATTCGTTATGT+TGG + chr2.4:14348020-14348039 MS.gene43083:CDS 50.0%
! GATCTCCAGCTTGTTCCCAT+CGG + chr2.4:14347400-14347419 MS.gene43083:CDS 50.0%
! GGAATTTTCCACCGGGAGAA+CGG - chr2.4:14347653-14347672 None:intergenic 50.0%
! GTCCAGGGTGTCATATCCAT+AGG - chr2.4:14348363-14348382 None:intergenic 50.0%
! TGAGCTTTGCCAATCGGTTC+GGG - chr2.4:14347263-14347282 None:intergenic 50.0%
! TGCAAAGTTTTCATCGGCGC+CGG - chr2.4:14347350-14347369 None:intergenic 50.0%
!! AAACGCGTTGGCTGAAGTTC+AGG + chr2.4:14348302-14348321 MS.gene43083:CDS 50.0%
CGAATCGGGTCACGTTACTC+GGG - chr2.4:14348014-14348033 None:intergenic 55.0%
CGTCGGAGAAGTTCAACTCC+CGG + chr2.4:14347729-14347748 MS.gene43083:CDS 55.0%
CGTTTCAAGCCGTCGAGCTT+TGG + chr2.4:14347614-14347633 MS.gene43083:CDS 55.0%
CTCCCGGTGGAAAATTCCCT+CGG + chr2.4:14347655-14347674 MS.gene43083:CDS 55.0%
CTTCTCCGACGATGTGGAGA+CGG - chr2.4:14347720-14347739 None:intergenic 55.0%
GCCACTTCGAATTGGTCGCA+AGG - chr2.4:14347595-14347614 None:intergenic 55.0%
GGAGGAGGAGGTCGTAGTTA+CGG - chr2.4:14347693-14347712 None:intergenic 55.0%
GGTTTCCGATGGGAACAAGC+TGG - chr2.4:14347408-14347427 None:intergenic 55.0%
GTTCCCATCGGAAACCTCAC+CGG + chr2.4:14347412-14347431 MS.gene43083:CDS 55.0%
TCCATAAAGCTCGGCAGCAC+GGG - chr2.4:14347959-14347978 None:intergenic 55.0%
TCTCCGGTGAGGTTTCCGAT+GGG - chr2.4:14347418-14347437 None:intergenic 55.0%
TTCTCCGGTGAGGTTTCCGA+TGG - chr2.4:14347419-14347438 None:intergenic 55.0%
! TGGATAAGCTTTTCCGGCGG+CGG - chr2.4:14347557-14347576 None:intergenic 55.0%
!! CCAATCGGTTCGGGTTGAAC+CGG - chr2.4:14347254-14347273 None:intergenic 55.0%
!! TTGAGGGTGTTGGAAGAGGG+AGG - chr2.4:14347133-14347152 None:intergenic 55.0%
ACTTCCACCGCCGTCAACAC+CGG + chr2.4:14347328-14347347 MS.gene43083:CDS 60.0%
AGTATGCTCCCGGGACTCCT+TGG - chr2.4:14347528-14347547 None:intergenic 60.0%
CCGGTGGAAAATTCCCTCGG+CGG + chr2.4:14347658-14347677 MS.gene43083:CDS 60.0%
CCGGTTCAACCCGAACCGAT+TGG + chr2.4:14347251-14347270 MS.gene43083:CDS 60.0%
CGACGATGTGGAGACGGTGA+CGG - chr2.4:14347714-14347733 None:intergenic 60.0%
CGTCACCGTCTCCACATCGT+CGG + chr2.4:14347712-14347731 MS.gene43083:CDS 60.0%
CGTCGCTTACCAAGGAGTCC+CGG + chr2.4:14347516-14347535 MS.gene43083:CDS 60.0%
CTGCTTCACGTGCGACGTTG+AGG - chr2.4:14347864-14347883 None:intergenic 60.0%
GGACTCCTTGGTAAGCGACG+CGG - chr2.4:14347516-14347535 None:intergenic 60.0%
GGAGAGCGAGGAGACAGTGA+TGG - chr2.4:14347756-14347775 None:intergenic 60.0%
GGAGGAGGTCGTAGTTACGG+TGG - chr2.4:14347690-14347709 None:intergenic 60.0%
GTCGCTTACCAAGGAGTCCC+GGG + chr2.4:14347517-14347536 MS.gene43083:CDS 60.0%
GTCGGCGATCCAAAGCTCGA+CGG - chr2.4:14347626-14347645 None:intergenic 60.0%
TGGCGATAGCTGCAGTGTCG+CGG - chr2.4:14347933-14347952 None:intergenic 60.0%
TTCTGATTCCGGGGAGAGCG+AGG - chr2.4:14347768-14347787 None:intergenic 60.0%
TTTCCACCGGGAGAACGGCA+CGG - chr2.4:14347648-14347667 None:intergenic 60.0%
! CCGCCGAGGGAATTTTCCAC+CGG - chr2.4:14347661-14347680 None:intergenic 60.0%
! CGCCGAGGGAATTTTCCACC+GGG - chr2.4:14347660-14347679 None:intergenic 60.0%
! TGACGATACAGCAGGTGCCG+CGG + chr2.4:14347885-14347904 MS.gene43083:CDS 60.0%
!! ACGGTGGATTGAACCGCCGA+GGG - chr2.4:14347674-14347693 None:intergenic 60.0%
!! AGGGTGTTGGAAGAGGGAGG+TGG - chr2.4:14347130-14347149 None:intergenic 60.0%
!! CGAGGAGACAGTGATGGACC+GGG - chr2.4:14347750-14347769 None:intergenic 60.0%
!! GCGAGGAGACAGTGATGGAC+CGG - chr2.4:14347751-14347770 None:intergenic 60.0%
!! TACGGTGGATTGAACCGCCG+AGG - chr2.4:14347675-14347694 None:intergenic 60.0%
!! TTCATCGGCGCCGGTGTTGA+CGG - chr2.4:14347341-14347360 None:intergenic 60.0%
AGCCTCCGCGTCGCTTACCA+AGG + chr2.4:14347508-14347527 MS.gene43083:CDS 65.0%
CACTGTCTCCTCGCTCTCCC+CGG + chr2.4:14347757-14347776 MS.gene43083:CDS 65.0%
CGGAGGCTTGAGCCGTGCAT+CGG - chr2.4:14347496-14347515 None:intergenic 65.0%
CGGCTTCGGAGTATGCTCCC+GGG - chr2.4:14347537-14347556 None:intergenic 65.0%
CTCCTTGGTAAGCGACGCGG+AGG - chr2.4:14347513-14347532 None:intergenic 65.0%
GCCCGTGCTGCCGAGCTTTA+TGG + chr2.4:14347955-14347974 MS.gene43083:CDS 65.0%
GCGGCTTCGGAGTATGCTCC+CGG - chr2.4:14347538-14347557 None:intergenic 65.0%
! AGCTTTTCCGGCGGCGGCTT+CGG - chr2.4:14347551-14347570 None:intergenic 65.0%
! CGATGTGGAGACGGTGACGG+AGG - chr2.4:14347711-14347730 None:intergenic 65.0%
! CTTGAGCCGTGCATCGGTGC+CGG - chr2.4:14347490-14347509 None:intergenic 65.0%
!! AAACCCGGTTCGGACCGAGC+CGG - chr2.4:14347198-14347217 None:intergenic 65.0%
!! AAACCGGCTCGGTCCGAACC+GGG + chr2.4:14347192-14347211 MS.gene43083:CDS 65.0%
!! CAAACCGGCTCGGTCCGAAC+CGG + chr2.4:14347191-14347210 MS.gene43083:CDS 65.0%
!! CATCGGTGCCGGAGCTAGGT+CGG - chr2.4:14347479-14347498 None:intergenic 65.0%
!! CTAGCTCCGGCACCGATGCA+CGG + chr2.4:14347481-14347500 MS.gene43083:CDS 65.0%
!! GGTGTTGACGGCGGTGGAAG+TGG - chr2.4:14347329-14347348 None:intergenic 65.0%
!! TGTGGAGACGGTGACGGAGG+AGG - chr2.4:14347708-14347727 None:intergenic 65.0%
CACCGGGAGAACGGCACGGT+CGG - chr2.4:14347644-14347663 None:intergenic 70.0%
GCATACTCCGAAGCCGCCGC+CGG + chr2.4:14347541-14347560 MS.gene43083:CDS 70.0%
! CGGTTCGGACCGAGCCGGTT+TGG - chr2.4:14347193-14347212 None:intergenic 70.0%
!! ATCGGCGCCGGTGTTGACGG+CGG - chr2.4:14347338-14347357 None:intergenic 70.0%
!! CGTGCATCGGTGCCGGAGCT+AGG - chr2.4:14347483-14347502 None:intergenic 70.0%
!! GGAGACGGTGACGGAGGAGG+AGG - chr2.4:14347705-14347724 None:intergenic 70.0%
AAGCTCGGCAGCACGGGCGC+TGG - chr2.4:14347953-14347972 None:intergenic 75.0%
! CGACCGTGCCGTTCTCCCGG+TGG + chr2.4:14347642-14347661 MS.gene43083:CDS 75.0%
! CGCCGACCGTGCCGTTCTCC+CGG + chr2.4:14347639-14347658 MS.gene43083:CDS 75.0%
!!! GGCGCCGGTGTTGACGGCGG+TGG - chr2.4:14347335-14347354 None:intergenic 80.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 14347112 14348392 14347112 ID=MS.gene43083
chr2.4 mRNA 14347112 14348392 14347112 ID=MS.gene43083.t1;Parent=MS.gene43083
chr2.4 exon 14347112 14348392 14347112 ID=MS.gene43083.t1.exon1;Parent=MS.gene43083.t1
chr2.4 CDS 14347112 14348392 14347112 ID=cds.MS.gene43083.t1;Parent=MS.gene43083.t1
Gene Sequence

>MS.gene43083

ATGAATACTCTATCTCCACCTCCCTCTTCCAACACCCTCAATTTCAACCACATAACAACAACACGGCCGTGGAAATTTCCAAACCGGCTCGGTCCGAACCGGGTTTCTGTCAAATGTGCTTACGGTTCAATTTATGAATCCGGTTCAACCCGAACCGATTGGCAAAGCTCATGTGCTATCTTATCTAGCAAAGTTGTTTCACAAGAACAATCTTCCACTTCCACCGCCGTCAACACCGGCGCCGATGAAAACTTTGCAGCTATCAACGGCCACAAAACAAACATAACCGATCTCCAGCTTGTTCCCATCGGAAACCTCACCGGAGAAATTTCAAAGCCACTTCCACCAAAACCGTTAACAATCTCCGACCTAGCTCCGGCACCGATGCACGGCTCAAGCCTCCGCGTCGCTTACCAAGGAGTCCCGGGAGCATACTCCGAAGCCGCCGCCGGAAAAGCTTATCCAAACTGCGAAGCAATTCCTTGCGACCAATTCGAAGTGGCGTTTCAAGCCGTCGAGCTTTGGATCGCCGACCGTGCCGTTCTCCCGGTGGAAAATTCCCTCGGCGGTTCAATCCACCGTAACTACGACCTCCTCCTCCGTCACCGTCTCCACATCGTCGGAGAAGTTCAACTCCCGGTCCATCACTGTCTCCTCGCTCTCCCCGGAATCAGAAAAGAATATCTGACACGTGTCATCTCTCATCCACAAGCTTTATCTCAATGCGAACACACATTAACAAAACTCGGCCTCAACGTCGCACGTGAAGCAGTTGACGATACAGCAGGTGCCGCGGAGTTTGTTGCAACTAACAATCTCCGCGACACTGCAGCTATCGCCAGCGCCCGTGCTGCCGAGCTTTATGGATTAAATATCTTAGCAGATGGAATTCAAGATGACCCGAGTAACGTGACCCGATTCGTTATGTTGGCCCGAGAACCAATAATTCCACGTACGGATCGTCCATTTAAAACAAGTATTGTTTTCGCTCATGATAAAGGAACTTCAGTGCTTTTCAAAGTGCTTTCAGCTTTTGCGTTTAGGAATATTAGTTTAACGAAGATTGAATCAAGGCCTCATCGTAACCGTCCGATTAGGCTTGTTGATGATGCAAATGTTGGAACTGCGAAGCACTTTGAGTATTTGTTTTATGTTGATTTTGAAGCTTCAATGGCTGAAGTTAGAGCACAAAACGCGTTGGCTGAAGTTCAGGAATTCACGTCTTTCTTGAGAGTTTTGGGTAGTTACCCTATGGATATGACACCCTGGACTACTCCTTAA
Protein sequence

>MS.gene43083.t1

MNTLSPPPSSNTLNFNHITTTRPWKFPNRLGPNRVSVKCAYGSIYESGSTRTDWQSSCAILSSKVVSQEQSSTSTAVNTGADENFAAINGHKTNITDLQLVPIGNLTGEISKPLPPKPLTISDLAPAPMHGSSLRVAYQGVPGAYSEAAAGKAYPNCEAIPCDQFEVAFQAVELWIADRAVLPVENSLGGSIHRNYDLLLRHRLHIVGEVQLPVHHCLLALPGIRKEYLTRVISHPQALSQCEHTLTKLGLNVAREAVDDTAGAAEFVATNNLRDTAAIASARAAELYGLNILADGIQDDPSNVTRFVMLAREPIIPRTDRPFKTSIVFAHDKGTSVLFKVLSAFAFRNISLTKIESRPHRNRPIRLVDDANVGTAKHFEYLFYVDFEASMAEVRAQNALAEVQEFTSFLRVLGSYPMDMTPWTTP