Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene43112.t1 | AES77968.1 | 98.9 | 188 | 2 | 0 | 1 | 188 | 1 | 188 | 2.90E-98 | 367.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene43112.t1 | P50346 | 68.3 | 104 | 32 | 1 | 7 | 110 | 11 | 113 | 6.9e-32 | 138.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene43112.t1 | G7KUS1 | 98.9 | 188 | 2 | 0 | 1 | 188 | 1 | 188 | 2.1e-98 | 367.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene054624 | MS.gene43112 | 0.809819 | 1.57E-50 | -1.69E-46 |
| MS.gene057321 | MS.gene43112 | 0.811155 | 8.07E-51 | -1.69E-46 |
| MS.gene060483 | MS.gene43112 | 0.802443 | 5.60E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene43112.t1 | MTR_7g022400 | 98.936 | 188 | 2 | 0 | 1 | 188 | 1 | 188 | 1.15e-139 | 387 |
| MS.gene43112.t1 | MTR_7g112750 | 81.818 | 187 | 16 | 1 | 1 | 187 | 1 | 169 | 1.79e-107 | 306 |
| MS.gene43112.t1 | MTR_3g108280 | 67.308 | 104 | 33 | 1 | 7 | 110 | 11 | 113 | 5.54e-41 | 141 |
| MS.gene43112.t1 | MTR_5g082180 | 66.346 | 104 | 34 | 1 | 7 | 110 | 11 | 113 | 2.06e-40 | 139 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene43112.t1 | AT2G40010 | 61.404 | 114 | 39 | 2 | 1 | 110 | 1 | 113 | 5.27e-38 | 133 |
| MS.gene43112.t1 | AT3G09200 | 63.462 | 104 | 37 | 1 | 7 | 110 | 10 | 112 | 7.73e-38 | 132 |
| MS.gene43112.t1 | AT3G11250 | 62.500 | 104 | 38 | 1 | 7 | 110 | 10 | 112 | 3.25e-37 | 131 |
| MS.gene43112.t1 | AT3G09200 | 42.308 | 104 | 26 | 2 | 7 | 110 | 10 | 79 | 1.82e-14 | 70.1 |
Find 48 sgRNAs with CRISPR-Local
Find 51 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGTTGGATCGAACCAGCTTC+AGG | 0.326070 | 7.2:-77233930 | MS.gene43112:CDS |
| CGAACCAGCTTCAGGGTATA+AGG | 0.338571 | 7.2:-77233922 | MS.gene43112:CDS |
| AGTTCTTGTTGACAAGTATA+TGG | 0.346686 | 7.2:-77233511 | MS.gene43112:CDS |
| TCGAGAGTACACACAAGTTT+TGG | 0.357954 | 7.2:-77233969 | MS.gene43112:CDS |
| ACCCAAGCAGCGGAGAAAAC+TGG | 0.386159 | 7.2:-77233836 | MS.gene43112:CDS |
| CGGAAGCTTGTGAAGATTGT+TGG | 0.393329 | 7.2:+77233597 | None:intergenic |
| CAAGGAGTGGAACGAGGTTA+AGG | 0.407384 | 7.2:+77233798 | None:intergenic |
| ATCTCATCATGTAACAGTGC+TGG | 0.426008 | 7.2:+77233540 | None:intergenic |
| AACCTCGTTCCACTCCTTGT+GGG | 0.430936 | 7.2:-77233794 | MS.gene43112:CDS |
| ATTTGGATCGGTAACATGGC+TGG | 0.436230 | 7.2:-77234037 | None:intergenic |
| GGCAGCTTACGACGCGAAGA+TGG | 0.466083 | 7.2:-77234002 | MS.gene43112:CDS |
| AACATCCCTCAAGTCACCTT+TGG | 0.471136 | 7.2:+77233751 | None:intergenic |
| GCAATCCGGACAGTTGCCAT+TGG | 0.473141 | 7.2:-77233636 | MS.gene43112:CDS |
| AGTCATATGACATGTACTTC+GGG | 0.478273 | 7.2:+77233681 | None:intergenic |
| TTACCAGCCAAAAGGAGAAA+AGG | 0.483227 | 7.2:+77233485 | None:intergenic |
| CGGTAACATGGCTGGGAAGG+TGG | 0.498093 | 7.2:-77234029 | None:intergenic |
| CAATCCGGACAGTTGCCATT+GGG | 0.503959 | 7.2:-77233635 | MS.gene43112:CDS |
| GTGGTTTCATCAGACAATGT+TGG | 0.507329 | 7.2:-77233947 | MS.gene43112:CDS |
| CTCTCCTTATACCCTGAAGC+TGG | 0.512942 | 7.2:+77233918 | None:intergenic |
| TAACCTCGTTCCACTCCTTG+TGG | 0.515777 | 7.2:-77233795 | MS.gene43112:CDS |
| GAGGCCCAATGGCAACTGTC+CGG | 0.527511 | 7.2:+77233631 | None:intergenic |
| GATCGGTAACATGGCTGGGA+AGG | 0.528105 | 7.2:-77234032 | None:intergenic |
| TACTTCGGGCACATGAGAAT+AGG | 0.529103 | 7.2:+77233695 | None:intergenic |
| TCTCATCATGTAACAGTGCT+GGG | 0.529636 | 7.2:+77233541 | None:intergenic |
| ACCTCGTTCCACTCCTTGTG+GGG | 0.531150 | 7.2:-77233793 | MS.gene43112:CDS |
| GTTGGATCGAACCAGCTTCA+GGG | 0.531277 | 7.2:-77233929 | MS.gene43112:CDS |
| ATCACAAATTACCAGCCAAA+AGG | 0.540624 | 7.2:+77233477 | None:intergenic |
| GAAGATTCAGTGGTTGTGAT+GGG | 0.554294 | 7.2:-77233887 | MS.gene43112:CDS |
| CATGGCTGGGAAGGTGGCGA+AGG | 0.555229 | 7.2:-77234023 | MS.gene43112:CDS |
| TTTGGATCGGTAACATGGCT+GGG | 0.555330 | 7.2:-77234036 | None:intergenic |
| GAGTCATATGACATGTACTT+CGG | 0.556970 | 7.2:+77233680 | None:intergenic |
| GCAGCTTACGACGCGAAGAT+GGG | 0.559567 | 7.2:-77234001 | MS.gene43112:CDS |
| ACTTGTTCCTACATGCAATC+CGG | 0.559664 | 7.2:-77233650 | MS.gene43112:CDS |
| TGAGCTTACAAGAAAAGGCT+CGG | 0.573435 | 7.2:+77233577 | None:intergenic |
| TGAACGAATTGCTAAGCTCA+AGG | 0.576611 | 7.2:-77233726 | MS.gene43112:CDS |
| GCAACGTTCCCCACAAGGAG+TGG | 0.578891 | 7.2:+77233785 | None:intergenic |
| TGAAGATTCAGTGGTTGTGA+TGG | 0.591106 | 7.2:-77233888 | MS.gene43112:CDS |
| CAACTGTCCGGATTGCATGT+AGG | 0.593652 | 7.2:+77233643 | None:intergenic |
| ATCATGTAACAGTGCTGGGG+TGG | 0.595286 | 7.2:+77233545 | None:intergenic |
| TTACAACATGTGAGGCCCAA+TGG | 0.599780 | 7.2:+77233620 | None:intergenic |
| TCAAAGCAACGTTCCCCACA+AGG | 0.602062 | 7.2:+77233780 | None:intergenic |
| GAACGAGGTTAAGGAAAGCA+TGG | 0.617046 | 7.2:+77233807 | None:intergenic |
| AGCACTGCATGAAGATTCAG+TGG | 0.637836 | 7.2:-77233897 | MS.gene43112:CDS |
| ATTGTTGGTTACAACATGTG+AGG | 0.653243 | 7.2:+77233612 | None:intergenic |
| CTCATCATGTAACAGTGCTG+GGG | 0.655453 | 7.2:+77233542 | None:intergenic |
| ACGCTCTATTACCCAAGCAG+CGG | 0.673282 | 7.2:-77233846 | MS.gene43112:CDS |
| TCCCCACAAGGAGTGGAACG+AGG | 0.691919 | 7.2:+77233792 | None:intergenic |
| AAGATTCAGTGGTTGTGATG+GGG | 0.703344 | 7.2:-77233886 | MS.gene43112:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATTTTGAGCTTACAAGAAA+AGG | + | chr7.2:77233931-77233950 | None:intergenic | 25.0% |
| AGTTCTTGTTGACAAGTATA+TGG | - | chr7.2:77233989-77234008 | MS.gene43112:CDS | 30.0% | |
| !! | GTTGCTTTGATTTTTACCAA+AGG | - | chr7.2:77233733-77233752 | MS.gene43112:CDS | 30.0% |
| AGTCATATGACATGTACTTC+GGG | + | chr7.2:77233822-77233841 | None:intergenic | 35.0% | |
| GAGTCATATGACATGTACTT+CGG | + | chr7.2:77233823-77233842 | None:intergenic | 35.0% | |
| ! | ATTGTTGGTTACAACATGTG+AGG | + | chr7.2:77233891-77233910 | None:intergenic | 35.0% |
| ! | TTTTACCAAAGGTGACTTGA+GGG | - | chr7.2:77233744-77233763 | MS.gene43112:CDS | 35.0% |
| ! | TTTTTACCAAAGGTGACTTG+AGG | - | chr7.2:77233743-77233762 | MS.gene43112:CDS | 35.0% |
| AAGATTCAGTGGTTGTGATG+GGG | - | chr7.2:77233614-77233633 | MS.gene43112:CDS | 40.0% | |
| ACTTGTTCCTACATGCAATC+CGG | - | chr7.2:77233850-77233869 | MS.gene43112:CDS | 40.0% | |
| ATCTCATCATGTAACAGTGC+TGG | + | chr7.2:77233963-77233982 | None:intergenic | 40.0% | |
| GAAGATTCAGTGGTTGTGAT+GGG | - | chr7.2:77233613-77233632 | MS.gene43112:CDS | 40.0% | |
| GTGGTTTCATCAGACAATGT+TGG | - | chr7.2:77233553-77233572 | MS.gene43112:CDS | 40.0% | |
| TCTCATCATGTAACAGTGCT+GGG | + | chr7.2:77233962-77233981 | None:intergenic | 40.0% | |
| TGAACGAATTGCTAAGCTCA+AGG | - | chr7.2:77233774-77233793 | MS.gene43112:CDS | 40.0% | |
| TGAAGATTCAGTGGTTGTGA+TGG | - | chr7.2:77233612-77233631 | MS.gene43112:CDS | 40.0% | |
| TGAGCTTACAAGAAAAGGCT+CGG | + | chr7.2:77233926-77233945 | None:intergenic | 40.0% | |
| TTACCAGCCAAAAGGAGAAA+AGG | + | chr7.2:77234018-77234037 | None:intergenic | 40.0% | |
| ! | ATGGTCTCCTTTTCTCCTTT+TGG | - | chr7.2:77234008-77234027 | MS.gene43112:CDS | 40.0% |
| !! | AGAGTACACACAAGTTTTGG+TGG | - | chr7.2:77233534-77233553 | MS.gene43112:CDS | 40.0% |
| !! | TCGAGAGTACACACAAGTTT+TGG | - | chr7.2:77233531-77233550 | MS.gene43112:CDS | 40.0% |
| AACATCCCTCAAGTCACCTT+TGG | + | chr7.2:77233752-77233771 | None:intergenic | 45.0% | |
| AGCACTGCATGAAGATTCAG+TGG | - | chr7.2:77233603-77233622 | MS.gene43112:CDS | 45.0% | |
| CGGAAGCTTGTGAAGATTGT+TGG | + | chr7.2:77233906-77233925 | None:intergenic | 45.0% | |
| CTCATCATGTAACAGTGCTG+GGG | + | chr7.2:77233961-77233980 | None:intergenic | 45.0% | |
| GAACGAGGTTAAGGAAAGCA+TGG | + | chr7.2:77233696-77233715 | None:intergenic | 45.0% | |
| TACTTCGGGCACATGAGAAT+AGG | + | chr7.2:77233808-77233827 | None:intergenic | 45.0% | |
| TTACAACATGTGAGGCCCAA+TGG | + | chr7.2:77233883-77233902 | None:intergenic | 45.0% | |
| ! | TCTCCTTTTCTCCTTTTGGC+TGG | - | chr7.2:77234012-77234031 | MS.gene43112:CDS | 45.0% |
| AACCTCGTTCCACTCCTTGT+GGG | - | chr7.2:77233706-77233725 | MS.gene43112:CDS | 50.0% | |
| ACGCTCTATTACCCAAGCAG+CGG | - | chr7.2:77233654-77233673 | MS.gene43112:CDS | 50.0% | |
| CAACTGTCCGGATTGCATGT+AGG | + | chr7.2:77233860-77233879 | None:intergenic | 50.0% | |
| CAAGGAGTGGAACGAGGTTA+AGG | + | chr7.2:77233705-77233724 | None:intergenic | 50.0% | |
| CAATCCGGACAGTTGCCATT+GGG | - | chr7.2:77233865-77233884 | MS.gene43112:CDS | 50.0% | |
| CGAACCAGCTTCAGGGTATA+AGG | - | chr7.2:77233578-77233597 | MS.gene43112:CDS | 50.0% | |
| CTCTCCTTATACCCTGAAGC+TGG | + | chr7.2:77233585-77233604 | None:intergenic | 50.0% | |
| GTTGGATCGAACCAGCTTCA+GGG | - | chr7.2:77233571-77233590 | MS.gene43112:CDS | 50.0% | |
| TAACCTCGTTCCACTCCTTG+TGG | - | chr7.2:77233705-77233724 | MS.gene43112:CDS | 50.0% | |
| TCAAAGCAACGTTCCCCACA+AGG | + | chr7.2:77233723-77233742 | None:intergenic | 50.0% | |
| TGTTGGATCGAACCAGCTTC+AGG | - | chr7.2:77233570-77233589 | MS.gene43112:CDS | 50.0% | |
| ! | ATCATGTAACAGTGCTGGGG+TGG | + | chr7.2:77233958-77233977 | None:intergenic | 50.0% |
| ! | TCCAGTTTTCTCCGCTGCTT+GGG | + | chr7.2:77233668-77233687 | None:intergenic | 50.0% |
| ! | TTCCAGTTTTCTCCGCTGCT+TGG | + | chr7.2:77233669-77233688 | None:intergenic | 50.0% |
| ACCCAAGCAGCGGAGAAAAC+TGG | - | chr7.2:77233664-77233683 | MS.gene43112:CDS | 55.0% | |
| ACCTCGTTCCACTCCTTGTG+GGG | - | chr7.2:77233707-77233726 | MS.gene43112:CDS | 55.0% | |
| GCAATCCGGACAGTTGCCAT+TGG | - | chr7.2:77233864-77233883 | MS.gene43112:CDS | 55.0% | |
| GCAGCTTACGACGCGAAGAT+GGG | - | chr7.2:77233499-77233518 | MS.gene43112:CDS | 55.0% | |
| GAGGCCCAATGGCAACTGTC+CGG | + | chr7.2:77233872-77233891 | None:intergenic | 60.0% | |
| GCAACGTTCCCCACAAGGAG+TGG | + | chr7.2:77233718-77233737 | None:intergenic | 60.0% | |
| GGCAGCTTACGACGCGAAGA+TGG | - | chr7.2:77233498-77233517 | MS.gene43112:CDS | 60.0% | |
| TCCCCACAAGGAGTGGAACG+AGG | + | chr7.2:77233711-77233730 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.2 | gene | 77233478 | 77234044 | 77233478 | ID=MS.gene43112 |
| chr7.2 | mRNA | 77233478 | 77234044 | 77233478 | ID=MS.gene43112.t1;Parent=MS.gene43112 |
| chr7.2 | exon | 77233478 | 77234044 | 77233478 | ID=MS.gene43112.t1.exon1;Parent=MS.gene43112.t1 |
| chr7.2 | CDS | 77233478 | 77234044 | 77233478 | ID=cds.MS.gene43112.t1;Parent=MS.gene43112.t1 |
| Gene Sequence |
| Protein sequence |
