Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene43209.t1 | XP_013447725.1 | 100 | 38 | 0 | 0 | 1 | 38 | 191 | 228 | 7.00E-10 | 72.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene43209.t1 | A0A072U7J6 | 100.0 | 38 | 0 | 0 | 1 | 38 | 191 | 228 | 5.0e-10 | 72.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene052896 | MS.gene43209 | 0.801783 | 7.65E-49 | -1.69E-46 |
| MS.gene053437 | MS.gene43209 | 0.826352 | 2.84E-54 | -1.69E-46 |
| MS.gene058168 | MS.gene43209 | 0.812373 | 4.38E-51 | -1.69E-46 |
| MS.gene058851 | MS.gene43209 | 0.808242 | 3.41E-50 | -1.69E-46 |
| MS.gene058906 | MS.gene43209 | 0.802615 | 5.16E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene43209.t1 | MTR_7g018370 | 100.000 | 52 | 0 | 0 | 1 | 52 | 191 | 242 | 3.81e-28 | 101 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 11 sgRNAs with CRISPR-Local
Find 10 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATGATGGATGGAGTTGAAGT+AGG | 0.491118 | 7.2:-79386291 | None:intergenic |
| AGAGGGAATGACAAGAGATG+TGG | 0.508546 | 7.2:+79386224 | None:intergenic |
| TTTAACCTCTCTATGATGGA+TGG | 0.516217 | 7.2:-79386303 | None:intergenic |
| TGATGGATGGAGTTGAAGTA+GGG | 0.547063 | 7.2:-79386290 | None:intergenic |
| GATGGAGTTGAAGTAGGGCT+AGG | 0.557032 | 7.2:-79386285 | None:intergenic |
| GAGGGAATGACAAGAGATGT+GGG | 0.562323 | 7.2:+79386225 | None:intergenic |
| TTTGCATTTGAATTTGAACT+AGG | 0.572177 | 7.2:-79386345 | None:intergenic |
| GGACTGCTTGAACTAAGATA+AGG | 0.574582 | 7.2:-79386264 | None:intergenic |
| CTGCTTGAACTAAGATAAGG+AGG | 0.612198 | 7.2:-79386261 | None:intergenic |
| CAACTCCATCCATCATAGAG+AGG | 0.653040 | 7.2:+79386298 | MS.gene43209:CDS |
| AGGGAATGACAAGAGATGTG+GGG | 0.701802 | 7.2:+79386226 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAATGCAAAAACAAAATCTG+AGG | + | chr7.2:79386359-79386378 | MS.gene43209:CDS | 25.0% |
| ! | TTTGCATTTGAATTTGAACT+AGG | - | chr7.2:79386348-79386367 | None:intergenic | 25.0% |
| ! | AGTTTTTAACCTCTCTATGA+TGG | - | chr7.2:79386310-79386329 | None:intergenic | 30.0% |
| ! | TTTAACCTCTCTATGATGGA+TGG | - | chr7.2:79386306-79386325 | None:intergenic | 35.0% |
| CTGCTTGAACTAAGATAAGG+AGG | - | chr7.2:79386264-79386283 | None:intergenic | 40.0% | |
| GGACTGCTTGAACTAAGATA+AGG | - | chr7.2:79386267-79386286 | None:intergenic | 40.0% | |
| ! | TGATGGATGGAGTTGAAGTA+GGG | - | chr7.2:79386293-79386312 | None:intergenic | 40.0% |
| !! | ATGATGGATGGAGTTGAAGT+AGG | - | chr7.2:79386294-79386313 | None:intergenic | 40.0% |
| CAACTCCATCCATCATAGAG+AGG | + | chr7.2:79386298-79386317 | MS.gene43209:CDS | 45.0% | |
| GATGGAGTTGAAGTAGGGCT+AGG | - | chr7.2:79386288-79386307 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.2 | gene | 79386231 | 79386386 | 79386231 | ID=MS.gene43209 |
| chr7.2 | mRNA | 79386231 | 79386386 | 79386231 | ID=MS.gene43209.t1;Parent=MS.gene43209 |
| chr7.2 | exon | 79386231 | 79386386 | 79386231 | ID=MS.gene43209.t1.exon1;Parent=MS.gene43209.t1 |
| chr7.2 | CDS | 79386231 | 79386386 | 79386231 | ID=cds.MS.gene43209.t1;Parent=MS.gene43209.t1 |
| Gene Sequence |
| Protein sequence |
