AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene053437


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene053437.t1 MTR_1g044580 98.667 150 2 0 3 152 141 290 2.26e-102 308
MS.gene053437.t1 MTR_1g073320 70.000 160 38 1 3 152 139 298 4.63e-71 217
MS.gene053437.t1 MTR_7g098410 47.651 149 76 2 5 152 135 282 1.39e-41 140
MS.gene053437.t1 MTR_1g080350 37.333 150 92 2 6 154 147 295 1.39e-27 104
MS.gene053437.t1 MTR_1g044560 43.689 103 58 0 4 106 24 126 2.62e-22 86.3
MS.gene053437.t1 MTR_8g104990 37.241 145 87 3 9 151 138 280 1.28e-19 83.2
MS.gene053437.t1 MTR_3g065370 38.384 99 56 2 53 149 177 272 5.27e-12 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene053437.t1 AT1G04520 64.072 167 45 3 3 154 141 307 3.45e-66 204
MS.gene053437.t1 AT2G33330 62.346 162 51 2 3 154 143 304 1.29e-63 197
MS.gene053437.t1 AT3G04370 41.611 149 84 2 6 153 172 318 1.56e-34 123
MS.gene053437.t1 AT3G04370 41.611 149 84 2 6 153 171 317 1.89e-34 122
MS.gene053437.t1 AT5G43980 39.752 161 87 3 3 154 141 300 2.84e-33 119
MS.gene053437.t1 AT3G60720 33.557 149 84 5 6 150 143 280 3.73e-16 73.9
MS.gene053437.t1 AT3G60720 33.793 145 81 5 6 146 143 276 1.51e-15 72.0
MS.gene053437.t1 AT2G01660 38.298 94 53 2 53 143 185 276 5.01e-12 62.4
MS.gene053437.t1 AT2G01660 38.298 94 53 2 53 143 185 276 5.29e-12 62.4

Find 28 sgRNAs with CRISPR-Local

Find 64 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCTTTATCAGCGATGGAAAA+TGG 0.195405 1.4:-29025298 MS.gene053437:CDS
GAATGTGGCAACTCTATTTC+TGG 0.253750 1.4:-29025145 MS.gene053437:CDS
CATAAGTGTTTCATTAGTTA+TGG 0.313486 1.4:-29025109 MS.gene053437:CDS
GCACAATGTGAAGGTGATAT+TGG 0.371110 1.4:-29025220 MS.gene053437:CDS
CTTTATCAGCGATGGAAAAT+GGG 0.376842 1.4:-29025297 MS.gene053437:CDS
AATGTTGCTGGAAGTGGTTT+TGG 0.382953 1.4:-29025337 MS.gene053437:CDS
TGTCTAGATGGAACTCCATT+AGG 0.401134 1.4:+29025079 None:intergenic
AATGGGGTTGTTAGTGGTCA+TGG 0.418001 1.4:-29025280 MS.gene053437:CDS
TATCTTGCACAGATTATTGA+AGG 0.421513 1.4:-29024279 None:intergenic
AGTTATGGCTATTATCCTAA+TGG 0.427351 1.4:-29025094 MS.gene053437:CDS
GCAATGACAGGGCAAGATCC+AGG 0.447894 1.4:-29024510 MS.gene053437:intron
ATAATACTAGGAGGAATTGC+TGG 0.462780 1.4:-29024474 MS.gene053437:CDS
TGTGGGATAACAAATGTTGC+TGG 0.470043 1.4:-29025349 MS.gene053437:CDS
GAATATGAAGAATGTCTAGA+TGG 0.484195 1.4:+29025067 None:intergenic
ATGGAAAATGGGGTTGTTAG+TGG 0.485449 1.4:-29025286 MS.gene053437:CDS
TTATATTCTTGCTATTTGCT+AGG 0.498339 1.4:-29024437 MS.gene053437:CDS
ATAACAAATGTTGCTGGAAG+TGG 0.510378 1.4:-29025343 MS.gene053437:CDS
AAAACAGCTGCTATAATACT+AGG 0.532912 1.4:-29024486 MS.gene053437:CDS
CAGATGCTCTATAAGACTTG+TGG 0.533238 1.4:-29025367 MS.gene053437:CDS
AGATGCTCTATAAGACTTGT+GGG 0.545982 1.4:-29025366 MS.gene053437:CDS
GGACGCTGCTTTATCAGCGA+TGG 0.565779 1.4:-29025305 MS.gene053437:CDS
ACTAGGAGGAATTGCTGGAG+TGG 0.599765 1.4:-29024469 MS.gene053437:CDS
GATATTGGTGACTCTGATTG+TGG 0.607730 1.4:-29025205 MS.gene053437:CDS
CAATGACAGGGCAAGATCCA+GGG 0.608246 1.4:-29024509 MS.gene053437:intron
CAGAGAGCTCAAGTTGAATG+TGG 0.627327 1.4:-29025160 MS.gene053437:CDS
ACAGCTGCTATAATACTAGG+AGG 0.630493 1.4:-29024483 MS.gene053437:CDS
TTTATCAGCGATGGAAAATG+GGG 0.640047 1.4:-29025296 MS.gene053437:CDS
AATTTGACAAAGAAACACAA+TGG 0.727029 1.4:-29024414 MS.gene053437:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAAAATATCAATTTAGTTCA+TGG - chr1.4:29024812-29024831 MS.gene053437:intron 15.0%
!!! TTTTTATACAACAAACTATA+TGG - chr1.4:29024404-29024423 MS.gene053437:intron 15.0%
!! TGATAATAATTTCAAACTGA+GGG - chr1.4:29024972-29024991 MS.gene053437:intron 20.0%
!!! ATTTTGAAATATTGATGATG+TGG - chr1.4:29025030-29025049 MS.gene053437:intron 20.0%
!!! TTGATAATAATTTCAAACTG+AGG - chr1.4:29024971-29024990 MS.gene053437:intron 20.0%
!!! TTTTGAAATATTGATGATGT+GGG - chr1.4:29025031-29025050 MS.gene053437:intron 20.0%
! AAATAAGTAAGTGGATGTAA+TGG - chr1.4:29024864-29024883 MS.gene053437:intron 25.0%
! AATTTGACAAAGAAACACAA+TGG - chr1.4:29025247-29025266 MS.gene053437:CDS 25.0%
! CATAAGTGTTTCATTAGTTA+TGG - chr1.4:29024552-29024571 MS.gene053437:intron 25.0%
! CTTAACCATTGGAAAATTTA+GGG + chr1.4:29025085-29025104 None:intergenic 25.0%
! TAACCAACATTTGTCATAAA+GGG + chr1.4:29024748-29024767 None:intergenic 25.0%
! TTAACCAACATTTGTCATAA+AGG + chr1.4:29024749-29024768 None:intergenic 25.0%
!! ATGTAATGGTTGATATTTGT+AGG - chr1.4:29024878-29024897 MS.gene053437:intron 25.0%
!! CTATATGGCATTTTATGTTT+TGG - chr1.4:29024419-29024438 MS.gene053437:CDS 25.0%
!!! AAAATAAGTGTCGATTTGTA+TGG - chr1.4:29024690-29024709 MS.gene053437:intron 25.0%
!!! CTTATTTTTGGTGATCAATT+TGG + chr1.4:29024851-29024870 None:intergenic 25.0%
!!! TACATCCACTTACTTATTTT+TGG + chr1.4:29024863-29024882 None:intergenic 25.0%
!!! TTATATTCTTGCTATTTGCT+AGG - chr1.4:29025224-29025243 MS.gene053437:CDS 25.0%
AAAACAGCTGCTATAATACT+AGG - chr1.4:29025175-29025194 MS.gene053437:CDS 30.0%
AGTTATGGCTATTATCCTAA+TGG - chr1.4:29024567-29024586 MS.gene053437:intron 30.0%
CCTTAACCATTGGAAAATTT+AGG + chr1.4:29025086-29025105 None:intergenic 30.0%
CTACACATTGAACCAAAATT+TGG - chr1.4:29024652-29024671 MS.gene053437:intron 30.0%
GAATATGAAGAATGTCTAGA+TGG + chr1.4:29024597-29024616 None:intergenic 30.0%
GATCACCAAAAATAAGTAAG+TGG - chr1.4:29024855-29024874 MS.gene053437:intron 30.0%
! CCTAAATTTTCCAATGGTTA+AGG - chr1.4:29025083-29025102 MS.gene053437:CDS 30.0%
! GAGATGAATTTGAAACTAAG+TGG - chr1.4:29024930-29024949 MS.gene053437:intron 30.0%
! TTTTCCAATGGTTAAGGAAA+AGG - chr1.4:29025089-29025108 MS.gene053437:CDS 30.0%
!! ATTGAACCAAAATTTGGTGT+TGG - chr1.4:29024658-29024677 MS.gene053437:intron 30.0%
!! ATTGATTTGATGCAATGACA+GGG - chr1.4:29025140-29025159 MS.gene053437:CDS 30.0%
!! TATTGATTTGATGCAATGAC+AGG - chr1.4:29025139-29025158 MS.gene053437:CDS 30.0%
!! TCATATTCGTCATTTTCTTC+AGG - chr1.4:29024609-29024628 MS.gene053437:intron 30.0%
!! TTGAACCAAAATTTGGTGTT+GGG - chr1.4:29024659-29024678 MS.gene053437:intron 30.0%
!!! CAAATTTTGGTTCAATGTGT+AGG + chr1.4:29024654-29024673 None:intergenic 30.0%
AACATTTGTCATAAAGGGCA+AGG + chr1.4:29024743-29024762 None:intergenic 35.0%
AGATGCTCTATAAGACTTGT+GGG - chr1.4:29024295-29024314 MS.gene053437:intron 35.0%
ATAACAAATGTTGCTGGAAG+TGG - chr1.4:29024318-29024337 MS.gene053437:intron 35.0%
ATAATACTAGGAGGAATTGC+TGG - chr1.4:29025187-29025206 MS.gene053437:CDS 35.0%
CTTTATCAGCGATGGAAAAT+GGG - chr1.4:29024364-29024383 MS.gene053437:intron 35.0%
TTGCCCTTTATGACAAATGT+TGG - chr1.4:29024742-29024761 MS.gene053437:intron 35.0%
TTTATCAGCGATGGAAAATG+GGG - chr1.4:29024365-29024384 MS.gene053437:intron 35.0%
! AGTACCTTTTCCTTAACCAT+TGG + chr1.4:29025096-29025115 None:intergenic 35.0%
! CTTTTCCCAACACCAAATTT+TGG + chr1.4:29024667-29024686 None:intergenic 35.0%
!! CAAAATTTGGTGTTGGGAAA+AGG - chr1.4:29024665-29024684 MS.gene053437:intron 35.0%
!!! TATGTTTTGGCACAATGTGA+AGG - chr1.4:29024432-29024451 MS.gene053437:CDS 35.0%
ACAGCTGCTATAATACTAGG+AGG - chr1.4:29025178-29025197 MS.gene053437:CDS 40.0%
ATGGAAAATGGGGTTGTTAG+TGG - chr1.4:29024375-29024394 MS.gene053437:intron 40.0%
CAGATGCTCTATAAGACTTG+TGG - chr1.4:29024294-29024313 MS.gene053437:intron 40.0%
GAATGTGGCAACTCTATTTC+TGG - chr1.4:29024516-29024535 MS.gene053437:intron 40.0%
GCACAATGTGAAGGTGATAT+TGG - chr1.4:29024441-29024460 MS.gene053437:CDS 40.0%
GCTTTATCAGCGATGGAAAA+TGG - chr1.4:29024363-29024382 MS.gene053437:intron 40.0%
TGTCTAGATGGAACTCCATT+AGG + chr1.4:29024585-29024604 None:intergenic 40.0%
TGTGGGATAACAAATGTTGC+TGG - chr1.4:29024312-29024331 MS.gene053437:intron 40.0%
! AATGTTGCTGGAAGTGGTTT+TGG - chr1.4:29024324-29024343 MS.gene053437:intron 40.0%
! CTGCTCCCTAAATTTTCCAA+TGG - chr1.4:29025077-29025096 MS.gene053437:CDS 40.0%
! GATATTGGTGACTCTGATTG+TGG - chr1.4:29024456-29024475 MS.gene053437:CDS 40.0%
!! ATTATAGCAGCTGTTTTCCC+TGG + chr1.4:29025172-29025191 None:intergenic 40.0%
AATGGGGTTGTTAGTGGTCA+TGG - chr1.4:29024381-29024400 MS.gene053437:intron 45.0%
!! CAGAGAGCTCAAGTTGAATG+TGG - chr1.4:29024501-29024520 MS.gene053437:CDS 45.0%
!!! AAGTGGTTTTGGCGAGAGAA+GGG - chr1.4:29024335-29024354 MS.gene053437:intron 45.0%
ACTAGGAGGAATTGCTGGAG+TGG - chr1.4:29025192-29025211 MS.gene053437:CDS 50.0%
CAATGACAGGGCAAGATCCA+GGG - chr1.4:29025152-29025171 MS.gene053437:CDS 50.0%
!!! GAAGTGGTTTTGGCGAGAGA+AGG - chr1.4:29024334-29024353 MS.gene053437:intron 50.0%
GCAATGACAGGGCAAGATCC+AGG - chr1.4:29025151-29025170 MS.gene053437:CDS 55.0%
! GGACGCTGCTTTATCAGCGA+TGG - chr1.4:29024356-29024375 MS.gene053437:intron 55.0%


Chromosome Type Strat End Strand Name
chr1.4 gene 29024282 29025401 29024282 ID=MS.gene053437
chr1.4 mRNA 29024282 29025401 29024282 ID=MS.gene053437.t1;Parent=MS.gene053437
chr1.4 exon 29025053 29025401 29025053 ID=MS.gene053437.t1.exon1;Parent=MS.gene053437.t1
chr1.4 CDS 29025053 29025401 29025053 ID=cds.MS.gene053437.t1;Parent=MS.gene053437.t1
chr1.4 exon 29024415 29024522 29024415 ID=MS.gene053437.t1.exon2;Parent=MS.gene053437.t1
chr1.4 CDS 29024415 29024522 29024415 ID=cds.MS.gene053437.t1;Parent=MS.gene053437.t1
chr1.4 exon 29024282 29024289 29024282 ID=MS.gene053437.t1.exon3;Parent=MS.gene053437.t1
chr1.4 CDS 29024282 29024289 29024282 ID=cds.MS.gene053437.t1;Parent=MS.gene053437.t1
Gene Sequence

>MS.gene053437

ATGTTAGGTATGCAGATGCTCTATAAGACTTGTGGGATAACAAATGTTGCTGGAAGTGGTTTTGGCGAGAGAAGGGACGCTGCTTTATCAGCGATGGAAAATGGGGTTGTTAGTGGTCATGGTTTTTATACAACAAACTATATGGCATTTTATGTTTTGGCACAATGTGAAGGTGATATTGGTGACTCTGATTGTGGACAGTGTGTAAAAACTGCTGTTCAGAGAGCTCAAGTTGAATGTGGCAACTCTATTTCTGGTGAAATCTTTTTGCATAAGTGTTTCATTAGTTATGGCTATTATCCTAATGGAGTTCCATCTAGACATTCTTCATATTCGTCATTTTCTTCAGGTAAGAACTAATGTTTAACACCTACACATTGAACCAAAATTTGGTGTTGGGAAAAGGTGAAAATAAGTGTCGATTTGTATGGTAAATTTTGACTAATCTGTCATACTGTCCTTGCCCTTTATGACAAATGTTGGTTAATTTCACAATGAGTAAATATATGAACTAGTCGATAAAATTGTTGTAAAATATCAATTTAGTTCATGGCATTATAGATAGTCCAAATTGATCACCAAAAATAAGTAAGTGGATGTAATGGTTGATATTTGTAGGACTAGAATGACATTAAGTTTGTAATCTCAGAGATGAATTTGAAACTAAGTGGATGTTGTCTGCGATGCATTTGATAATAATTTCAAACTGAGGGACAAACTTGATTAAAATTTTGTAATTCTAGACATCATTTTGAAATATTGATGATGTGGGATACTAGATTGATTAAAATAATTCTGCTCCCTAAATTTTCCAATGGTTAAGGAAAAGGTACTAGTCTTCTATCTAAGTATGATCATATTGATTTGATGCAATGACAGGGCAAGATCCAGGGAAAACAGCTGCTATAATACTAGGAGGAATTGCTGGAGTGGCTTTTCTAGTTATATTCTTGCTATTTGCTAGGAATTTGACAAAGAAACACAATGGTATGTATGAGTTATTTATGAAGTCTTATGTTTATATTTTAGTCTCATGTTTTGATTCTTTTTTGAAGAAACTCATGTTTTGATTCTTATTTACTCATTTTGCAATATCATTTTATCTTGCACAGATTATTGA

Protein sequence

>MS.gene053437.t1

MLGMQMLYKTCGITNVAGSGFGERRDAALSAMENGVVSGHGFYTTNYMAFYVLAQCEGDIGDSDCGQCVKTAVQRAQVECGNSISGEIFLHKCFISYGYYPNGVPSRHSSYSSFSSGQDPGKTAAIILGGIAGVAFLVIFLLFARNLTKKHNDY