Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene43218.t1 | XP_013457856.1 | 96.2 | 520 | 16 | 1 | 1 | 516 | 1 | 520 | 3.10E-283 | 983.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene43218.t1 | Q9FLD5 | 57.3 | 468 | 187 | 4 | 5 | 466 | 3 | 463 | 1.3e-152 | 541.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene43218.t1 | A0A072UQF5 | 96.2 | 520 | 16 | 1 | 1 | 516 | 1 | 520 | 2.2e-283 | 983.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene055251 | MS.gene43218 | 0.823243 | 1.54E-53 | -1.69E-46 |
| MS.gene056266 | MS.gene43218 | -0.843439 | 1.40E-58 | -1.69E-46 |
| MS.gene061044 | MS.gene43218 | 0.820735 | 5.86E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene43218.t1 | MTR_4g107370 | 97.692 | 520 | 8 | 1 | 1 | 516 | 1 | 520 | 0.0 | 1055 |
| MS.gene43218.t1 | MTR_1g022250 | 62.236 | 474 | 164 | 3 | 5 | 477 | 2 | 461 | 0.0 | 604 |
| MS.gene43218.t1 | MTR_7g008040 | 61.905 | 462 | 172 | 3 | 6 | 466 | 1 | 459 | 0.0 | 596 |
| MS.gene43218.t1 | MTR_2g030420 | 61.088 | 478 | 166 | 3 | 1 | 477 | 1 | 459 | 0.0 | 593 |
| MS.gene43218.t1 | MTR_6g009540 | 59.914 | 464 | 175 | 5 | 6 | 466 | 3 | 458 | 0.0 | 563 |
| MS.gene43218.t1 | MTR_6g009520 | 59.140 | 465 | 179 | 4 | 5 | 466 | 4 | 460 | 0.0 | 562 |
| MS.gene43218.t1 | MTR_6g009720 | 57.234 | 470 | 186 | 5 | 6 | 466 | 5 | 468 | 0.0 | 538 |
| MS.gene43218.t1 | MTR_1g022245 | 54.881 | 461 | 203 | 3 | 8 | 466 | 3 | 460 | 0.0 | 526 |
| MS.gene43218.t1 | MTR_6g009553 | 58.000 | 450 | 171 | 6 | 29 | 466 | 4 | 447 | 0.0 | 516 |
| MS.gene43218.t1 | MTR_6g009770 | 54.777 | 471 | 174 | 8 | 6 | 466 | 5 | 446 | 4.10e-171 | 492 |
| MS.gene43218.t1 | MTR_6g042290 | 53.996 | 463 | 175 | 7 | 13 | 468 | 21 | 452 | 3.02e-168 | 485 |
| MS.gene43218.t1 | MTR_6g009510 | 49.891 | 459 | 155 | 7 | 13 | 468 | 21 | 407 | 8.85e-147 | 429 |
| MS.gene43218.t1 | MTR_6g009660 | 46.695 | 469 | 176 | 10 | 9 | 466 | 5 | 410 | 2.79e-130 | 387 |
| MS.gene43218.t1 | MTR_4g128260 | 41.548 | 491 | 262 | 10 | 6 | 485 | 1 | 477 | 1.05e-122 | 370 |
| MS.gene43218.t1 | MTR_2g076970 | 42.060 | 466 | 223 | 9 | 12 | 470 | 6 | 431 | 3.81e-121 | 363 |
| MS.gene43218.t1 | MTR_2g076920 | 40.625 | 512 | 242 | 12 | 5 | 500 | 3 | 468 | 3.30e-120 | 362 |
| MS.gene43218.t1 | MTR_2g076690 | 41.042 | 480 | 234 | 7 | 15 | 485 | 16 | 455 | 2.17e-117 | 354 |
| MS.gene43218.t1 | MTR_2g076710 | 41.164 | 464 | 229 | 9 | 15 | 470 | 14 | 441 | 4.61e-117 | 353 |
| MS.gene43218.t1 | MTR_2g076840 | 42.038 | 471 | 224 | 9 | 13 | 475 | 7 | 436 | 3.64e-116 | 359 |
| MS.gene43218.t1 | MTR_2g076840 | 51.136 | 264 | 101 | 2 | 203 | 466 | 467 | 702 | 1.25e-84 | 277 |
| MS.gene43218.t1 | MTR_5g020980 | 37.773 | 503 | 268 | 9 | 7 | 498 | 11 | 479 | 1.71e-114 | 348 |
| MS.gene43218.t1 | MTR_2g076900 | 40.486 | 494 | 238 | 12 | 15 | 500 | 9 | 454 | 5.96e-114 | 345 |
| MS.gene43218.t1 | MTR_2g061820 | 40.162 | 493 | 259 | 11 | 6 | 492 | 4 | 466 | 8.22e-112 | 342 |
| MS.gene43218.t1 | MTR_2g076990 | 43.256 | 430 | 198 | 9 | 66 | 486 | 1 | 393 | 3.99e-110 | 335 |
| MS.gene43218.t1 | MTR_8g076920 | 36.614 | 508 | 277 | 8 | 9 | 501 | 23 | 500 | 1.83e-105 | 325 |
| MS.gene43218.t1 | MTR_5g020990 | 34.375 | 480 | 265 | 10 | 10 | 470 | 25 | 473 | 7.84e-89 | 281 |
| MS.gene43218.t1 | MTR_3g103150 | 42.903 | 310 | 135 | 5 | 161 | 466 | 4 | 275 | 3.64e-79 | 249 |
| MS.gene43218.t1 | MTR_3g092060 | 29.945 | 541 | 266 | 16 | 9 | 496 | 2 | 482 | 2.89e-66 | 222 |
| MS.gene43218.t1 | MTR_7g117950 | 31.765 | 425 | 224 | 10 | 46 | 466 | 42 | 404 | 5.64e-65 | 218 |
| MS.gene43218.t1 | MTR_5g021020 | 34.294 | 347 | 172 | 9 | 124 | 468 | 114 | 406 | 1.03e-52 | 184 |
| MS.gene43218.t1 | MTR_8g027200 | 28.817 | 465 | 237 | 13 | 35 | 480 | 29 | 418 | 6.10e-51 | 181 |
| MS.gene43218.t1 | MTR_1g011680 | 51.807 | 166 | 78 | 1 | 338 | 501 | 3 | 168 | 6.66e-45 | 156 |
| MS.gene43218.t1 | MTR_5g021000 | 47.200 | 125 | 61 | 2 | 362 | 484 | 1 | 122 | 6.80e-31 | 118 |
| MS.gene43218.t1 | MTR_5g020720 | 39.326 | 178 | 79 | 5 | 268 | 443 | 26 | 176 | 7.45e-27 | 107 |
| MS.gene43218.t1 | MTR_1g113750 | 47.368 | 95 | 50 | 0 | 395 | 489 | 16 | 110 | 2.81e-20 | 87.0 |
| MS.gene43218.t1 | MTR_5g021010 | 46.667 | 90 | 43 | 2 | 379 | 466 | 34 | 120 | 5.84e-18 | 80.5 |
| MS.gene43218.t1 | MTR_2g076820 | 52.174 | 69 | 30 | 1 | 395 | 463 | 1 | 66 | 8.74e-16 | 72.8 |
| MS.gene43218.t1 | MTR_7g010800 | 28.372 | 215 | 122 | 8 | 207 | 415 | 206 | 394 | 1.25e-12 | 70.9 |
| MS.gene43218.t1 | MTR_4g094662 | 28.505 | 214 | 121 | 8 | 208 | 415 | 230 | 417 | 1.63e-12 | 70.5 |
| MS.gene43218.t1 | MTR_2g438140 | 28.037 | 214 | 122 | 8 | 208 | 415 | 227 | 414 | 4.24e-12 | 69.3 |
| MS.gene43218.t1 | MTR_2g438140 | 28.037 | 214 | 122 | 8 | 208 | 415 | 287 | 474 | 5.59e-12 | 68.6 |
| MS.gene43218.t1 | MTR_2g101290 | 29.630 | 189 | 104 | 5 | 223 | 408 | 430 | 592 | 1.65e-11 | 67.4 |
| MS.gene43218.t1 | MTR_2g101290 | 29.630 | 189 | 104 | 5 | 223 | 408 | 430 | 592 | 2.29e-11 | 67.0 |
| MS.gene43218.t1 | MTR_4g094270 | 27.919 | 197 | 114 | 6 | 207 | 400 | 156 | 327 | 4.54e-11 | 65.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene43218.t1 | AT5G40010 | 57.634 | 465 | 184 | 4 | 5 | 463 | 3 | 460 | 0.0 | 562 |
| MS.gene43218.t1 | AT3G28580 | 57.419 | 465 | 178 | 7 | 6 | 463 | 4 | 455 | 0.0 | 529 |
| MS.gene43218.t1 | AT3G28510 | 54.212 | 463 | 203 | 3 | 9 | 470 | 7 | 461 | 2.49e-176 | 507 |
| MS.gene43218.t1 | AT3G28540 | 54.746 | 453 | 195 | 3 | 14 | 465 | 12 | 455 | 1.53e-173 | 499 |
| MS.gene43218.t1 | AT3G28540 | 54.746 | 453 | 195 | 3 | 14 | 465 | 12 | 455 | 1.92e-173 | 499 |
| MS.gene43218.t1 | AT3G28580 | 53.879 | 464 | 174 | 7 | 6 | 463 | 4 | 433 | 7.77e-169 | 486 |
| MS.gene43218.t1 | AT3G28600 | 52.101 | 476 | 185 | 7 | 9 | 470 | 3 | 449 | 7.81e-166 | 478 |
| MS.gene43218.t1 | AT3G28610 | 49.507 | 507 | 197 | 9 | 9 | 496 | 2 | 468 | 1.73e-164 | 474 |
| MS.gene43218.t1 | AT5G40000 | 48.770 | 488 | 196 | 11 | 12 | 478 | 10 | 464 | 3.20e-154 | 448 |
| MS.gene43218.t1 | AT3G28520 | 50.881 | 454 | 206 | 4 | 17 | 466 | 1 | 441 | 9.08e-153 | 445 |
| MS.gene43218.t1 | AT3G28600 | 48.739 | 476 | 162 | 6 | 9 | 470 | 3 | 410 | 9.31e-149 | 433 |
| MS.gene43218.t1 | AT3G28570 | 47.269 | 476 | 197 | 7 | 1 | 466 | 1 | 432 | 4.53e-146 | 427 |
| MS.gene43218.t1 | AT3G28570 | 47.269 | 476 | 197 | 7 | 1 | 466 | 8 | 439 | 5.92e-146 | 427 |
| MS.gene43218.t1 | AT5G57480 | 42.268 | 485 | 249 | 10 | 6 | 475 | 1 | 469 | 3.02e-121 | 366 |
| MS.gene43218.t1 | AT3G50940 | 42.257 | 452 | 217 | 8 | 25 | 470 | 31 | 444 | 4.13e-121 | 363 |
| MS.gene43218.t1 | AT4G25835 | 42.572 | 451 | 233 | 8 | 6 | 447 | 1 | 434 | 1.84e-120 | 363 |
| MS.gene43218.t1 | AT3G50930 | 41.434 | 502 | 258 | 9 | 21 | 516 | 73 | 544 | 3.05e-120 | 365 |
| MS.gene43218.t1 | AT4G30250 | 41.309 | 489 | 257 | 9 | 5 | 480 | 2 | 473 | 5.46e-112 | 342 |
| MS.gene43218.t1 | AT4G30250 | 41.189 | 488 | 257 | 9 | 6 | 480 | 1 | 471 | 2.06e-111 | 340 |
| MS.gene43218.t1 | AT2G18193 | 39.425 | 487 | 251 | 11 | 15 | 494 | 19 | 468 | 6.89e-109 | 333 |
| MS.gene43218.t1 | AT5G17760 | 39.909 | 441 | 209 | 7 | 69 | 489 | 72 | 476 | 6.66e-103 | 318 |
| MS.gene43218.t1 | AT2G18190 | 37.895 | 475 | 257 | 8 | 2 | 472 | 8 | 448 | 4.10e-102 | 316 |
| MS.gene43218.t1 | AT5G17740 | 36.276 | 521 | 274 | 9 | 9 | 515 | 13 | 489 | 6.83e-101 | 314 |
| MS.gene43218.t1 | AT4G05380 | 37.179 | 468 | 245 | 11 | 15 | 470 | 20 | 450 | 1.19e-98 | 306 |
| MS.gene43218.t1 | AT5G17730 | 35.537 | 484 | 264 | 8 | 1 | 472 | 1 | 448 | 2.29e-96 | 300 |
| MS.gene43218.t1 | AT1G43910 | 37.527 | 461 | 250 | 10 | 21 | 473 | 26 | 456 | 1.22e-92 | 291 |
| MS.gene43218.t1 | AT5G17750 | 40.379 | 369 | 181 | 5 | 73 | 432 | 52 | 390 | 1.12e-90 | 283 |
| MS.gene43218.t1 | AT2G46620 | 32.328 | 464 | 256 | 8 | 9 | 466 | 4 | 415 | 1.61e-74 | 244 |
| MS.gene43218.t1 | AT5G17760 | 39.179 | 268 | 139 | 2 | 69 | 321 | 72 | 330 | 1.30e-57 | 195 |
| MS.gene43218.t1 | AT3G28560 | 41.395 | 215 | 116 | 3 | 9 | 220 | 7 | 214 | 2.39e-51 | 176 |
| MS.gene43218.t1 | AT3G29800 | 30.890 | 382 | 198 | 10 | 109 | 484 | 76 | 397 | 1.25e-46 | 168 |
| MS.gene43218.t1 | AT4G05340 | 48.889 | 90 | 38 | 1 | 338 | 419 | 4 | 93 | 3.82e-20 | 85.9 |
| MS.gene43218.t1 | AT5G15250 | 28.079 | 203 | 118 | 7 | 208 | 408 | 222 | 398 | 4.71e-12 | 68.9 |
| MS.gene43218.t1 | AT2G30950 | 28.037 | 214 | 122 | 8 | 208 | 415 | 225 | 412 | 6.15e-12 | 68.6 |
| MS.gene43218.t1 | AT2G30950 | 28.037 | 214 | 122 | 8 | 208 | 415 | 225 | 412 | 6.15e-12 | 68.6 |
| MS.gene43218.t1 | AT2G30950 | 28.037 | 214 | 122 | 8 | 208 | 415 | 225 | 412 | 6.15e-12 | 68.6 |
| MS.gene43218.t1 | AT2G30950 | 28.037 | 214 | 122 | 8 | 208 | 415 | 229 | 416 | 7.09e-12 | 68.6 |
| MS.gene43218.t1 | AT5G15250 | 28.866 | 194 | 110 | 7 | 208 | 399 | 222 | 389 | 1.57e-11 | 67.0 |
| MS.gene43218.t1 | AT1G06430 | 27.586 | 203 | 119 | 7 | 208 | 408 | 218 | 394 | 1.76e-11 | 67.4 |
| MS.gene43218.t1 | AT1G06430 | 27.586 | 203 | 119 | 7 | 208 | 408 | 218 | 394 | 1.76e-11 | 67.4 |
| MS.gene43218.t1 | AT1G06430 | 27.586 | 203 | 119 | 7 | 208 | 408 | 218 | 394 | 1.76e-11 | 67.4 |
| MS.gene43218.t1 | AT5G19990 | 27.919 | 197 | 114 | 6 | 207 | 400 | 158 | 329 | 6.36e-11 | 64.7 |
| MS.gene43218.t1 | AT5G19990 | 27.919 | 197 | 114 | 6 | 207 | 400 | 158 | 329 | 6.36e-11 | 64.7 |
| MS.gene43218.t1 | AT5G19990 | 27.919 | 197 | 114 | 6 | 207 | 400 | 100 | 271 | 6.79e-11 | 64.3 |
| MS.gene43218.t1 | AT5G20000 | 27.919 | 197 | 114 | 6 | 207 | 400 | 158 | 329 | 7.07e-11 | 64.7 |
Find 85 sgRNAs with CRISPR-Local
Find 105 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AATAGCAAAGTGACACTTTC+AGG | 0.150044 | 8.4:-19534460 | MS.gene43218:CDS |
| TACAATAACAGGTTCATATT+TGG | 0.168811 | 8.4:-19535043 | MS.gene43218:CDS |
| TTCTCTTGCTCTTGATAATA+AGG | 0.193736 | 8.4:+19535105 | None:intergenic |
| GAAAGAGATTATTGAAGATT+TGG | 0.268353 | 8.4:-19534851 | MS.gene43218:CDS |
| GATTGTTCACTTGATCTTAC+AGG | 0.280503 | 8.4:-19534595 | MS.gene43218:CDS |
| TGAATTTCATTGATGGAATA+TGG | 0.281171 | 8.4:-19534432 | MS.gene43218:CDS |
| TAGCAAGTTTCATGTTCATT+TGG | 0.286672 | 8.4:-19535458 | MS.gene43218:CDS |
| GTTGGTCCTATTAAGGAAAA+TGG | 0.291304 | 8.4:-19534037 | MS.gene43218:CDS |
| ATAAAGGTGTCAAGGTTTAT+TGG | 0.304406 | 8.4:-19535164 | MS.gene43218:CDS |
| TGCTGCTATGGCTAATTTGT+TGG | 0.304769 | 8.4:-19534725 | MS.gene43218:CDS |
| GGAACATGTTGGTCCTATTA+AGG | 0.313426 | 8.4:-19534044 | MS.gene43218:CDS |
| CCACATTTCAGTCAATTTGT+AGG | 0.319479 | 8.4:+19535500 | None:intergenic |
| GAGAGTATTGAGAAGCTAAT+TGG | 0.324876 | 8.4:-19534232 | MS.gene43218:CDS |
| ATAATCTCTTTCTTCTTCTC+AGG | 0.331857 | 8.4:+19534862 | None:intergenic |
| ATCTCATTCCATGAATTCTT+AGG | 0.343436 | 8.4:-19535342 | MS.gene43218:CDS |
| AGCAAGTTTCATGTTCATTT+GGG | 0.345653 | 8.4:-19535457 | MS.gene43218:CDS |
| TGATGATGAGAATGAAAATA+AGG | 0.348942 | 8.4:-19534536 | MS.gene43218:CDS |
| TAAAGGTGTCAAGGTTTATT+GGG | 0.349534 | 8.4:-19535163 | MS.gene43218:CDS |
| GTCTGTCACCTAAGAATTCA+TGG | 0.358843 | 8.4:+19535334 | None:intergenic |
| AGCTTGATCCTGCACTTATA+AGG | 0.377934 | 8.4:-19534357 | MS.gene43218:CDS |
| GCCATTGTTTCAAAAGTTGC+AGG | 0.380424 | 8.4:+19534892 | None:intergenic |
| GGGCTATTGAATTTCATTGA+TGG | 0.383780 | 8.4:-19534439 | MS.gene43218:CDS |
| GCTGCTATGGCTAATTTGTT+GGG | 0.384550 | 8.4:-19534724 | MS.gene43218:CDS |
| CACATAGTTTGCTTATAGCT+TGG | 0.413731 | 8.4:+19534937 | None:intergenic |
| CTCAAAAGAAGTGATGCTTA+TGG | 0.418223 | 8.4:-19535312 | MS.gene43218:CDS |
| ATCAACAATGATTGCTGCTA+TGG | 0.421593 | 8.4:-19534737 | MS.gene43218:CDS |
| GGGTTATGATGTTTATGATT+TGG | 0.423960 | 8.4:-19534704 | MS.gene43218:CDS |
| AGGTGAAGGAAGAAGGAAGT+GGG | 0.424613 | 8.4:-19534489 | MS.gene43218:CDS |
| AGGAAGGAGGTGAAGGAAGA+AGG | 0.430248 | 8.4:-19534496 | MS.gene43218:CDS |
| TTTCAAAGAAACGTAGAAGT+TGG | 0.437255 | 8.4:+19535409 | None:intergenic |
| AAGAGACTCAAAGCTGAGAT+TGG | 0.441694 | 8.4:-19535246 | MS.gene43218:CDS |
| TGGAATGAGATTCTTATGTA+AGG | 0.443190 | 8.4:+19535354 | None:intergenic |
| AAGTATAGAGATACAATAAC+AGG | 0.445450 | 8.4:-19535054 | MS.gene43218:CDS |
| AGAGTAACTGATGATTATAA+AGG | 0.449383 | 8.4:-19535180 | MS.gene43218:CDS |
| AATTGGAAAAGCTTGGAAAA+GGG | 0.451620 | 8.4:-19534794 | MS.gene43218:CDS |
| ATGCTAGAATTGGAAAAGCT+TGG | 0.456038 | 8.4:-19534801 | MS.gene43218:CDS |
| TTGGATCATGTGATGAAAGA+AGG | 0.464493 | 8.4:-19535024 | MS.gene43218:CDS |
| GAATTGGAAAAGCTTGGAAA+AGG | 0.468849 | 8.4:-19534795 | MS.gene43218:CDS |
| GAGGTGAAGGAAGAAGGAAG+TGG | 0.470118 | 8.4:-19534490 | MS.gene43218:CDS |
| AAGGAAAATGGTGAGGTTGT+TGG | 0.472585 | 8.4:-19534025 | MS.gene43218:CDS |
| GTGAAGGAAGAAGGAAGTGG+GGG | 0.488319 | 8.4:-19534487 | MS.gene43218:CDS |
| TGGAAAAGATAGCACCAACT+TGG | 0.490393 | 8.4:-19535226 | MS.gene43218:CDS |
| CAAACAGTCCTGGTTACAAA+TGG | 0.491886 | 8.4:-19534960 | MS.gene43218:CDS |
| TGGATCATGTGATGAAAGAA+GGG | 0.494339 | 8.4:-19535023 | MS.gene43218:CDS |
| ACTGAAATGTGGACAACAAT+GGG | 0.495185 | 8.4:-19535489 | MS.gene43218:CDS |
| TACTCTTTCATATTCATCCA+TGG | 0.501783 | 8.4:+19535197 | None:intergenic |
| AAAATACAGTCACAGAATCA+TGG | 0.502989 | 8.4:-19535388 | MS.gene43218:CDS |
| ATTGTTGATTTACCAGTACC+TGG | 0.512918 | 8.4:+19534751 | None:intergenic |
| ATTGGAAAAGCTTGGAAAAG+GGG | 0.514100 | 8.4:-19534793 | MS.gene43218:CDS |
| TGCTGTGAGGAAGGAGGTGA+AGG | 0.515156 | 8.4:-19534503 | MS.gene43218:CDS |
| CTACCCCTCCTTATAAGTGC+AGG | 0.519905 | 8.4:+19534349 | None:intergenic |
| ACTTATAAGGAGGGGTAGAA+TGG | 0.522112 | 8.4:-19534344 | MS.gene43218:CDS |
| AAGGGAAAGAGATTCGTTTG+AGG | 0.523702 | 8.4:-19535005 | MS.gene43218:CDS |
| AAGAAACGTAGAAGTTGGTA+TGG | 0.524293 | 8.4:+19535414 | None:intergenic |
| GGTGAAGGAAGAAGGAAGTG+GGG | 0.528598 | 8.4:-19534488 | MS.gene43218:CDS |
| AGATTCTCAGCAACATCAGC+AGG | 0.533263 | 8.4:+19534193 | None:intergenic |
| TAAAGAACAAGATGAGGAAG+TGG | 0.537409 | 8.4:-19534065 | MS.gene43218:CDS |
| GCATCATCCATTGGAGACTT+AGG | 0.537455 | 8.4:+19534166 | None:intergenic |
| CACTTATCAGCATCATCCAT+TGG | 0.541958 | 8.4:+19534157 | None:intergenic |
| CACTTGATCTTACAGGTCAA+AGG | 0.546145 | 8.4:-19534588 | MS.gene43218:CDS |
| CCTACAAATTGACTGAAATG+TGG | 0.548791 | 8.4:-19535500 | MS.gene43218:CDS |
| ATAGCAAAGTGACACTTTCA+GGG | 0.551851 | 8.4:-19534459 | MS.gene43218:CDS |
| ATCCATGGTTAAAACCAAGT+TGG | 0.556634 | 8.4:+19535212 | None:intergenic |
| TAGCTTGGCCATTTGTAACC+AGG | 0.556681 | 8.4:+19534952 | None:intergenic |
| TTTGGAGTTAACAGCAGTGA+AGG | 0.569744 | 8.4:-19534686 | MS.gene43218:CDS |
| TTTAATTAAAGAACAAGATG+AGG | 0.572969 | 8.4:-19534071 | MS.gene43218:CDS |
| TCTTATGCCTAAGTCTCCAA+TGG | 0.574824 | 8.4:-19534173 | MS.gene43218:CDS |
| AATGAACATGAAACTTGCTA+AGG | 0.578436 | 8.4:+19535461 | None:intergenic |
| GACTGAAATGTGGACAACAA+TGG | 0.586419 | 8.4:-19535490 | MS.gene43218:CDS |
| TGATCCTGCACTTATAAGGA+GGG | 0.596373 | 8.4:-19534353 | MS.gene43218:CDS |
| TGATGATTATAAAGGTGTCA+AGG | 0.596926 | 8.4:-19535172 | MS.gene43218:CDS |
| TTGCTCTTGATAATAAGGCA+TGG | 0.598551 | 8.4:+19535110 | None:intergenic |
| TTGATCCTGCACTTATAAGG+AGG | 0.600419 | 8.4:-19534354 | MS.gene43218:CDS |
| TCCTATTAAGGAAAATGGTG+AGG | 0.608727 | 8.4:-19534032 | MS.gene43218:CDS |
| AAATTGTATACAAACAGTCC+TGG | 0.610851 | 8.4:-19534970 | MS.gene43218:CDS |
| TTCGTTTGAGGAACAGACAG+AGG | 0.628162 | 8.4:-19534993 | MS.gene43218:CDS |
| AAGCAATGAAGCAAATTCAG+AGG | 0.635171 | 8.4:-19533982 | MS.gene43218:CDS |
| GGTCAGCTTGTGGAGGTGAA+AGG | 0.651822 | 8.4:-19534411 | MS.gene43218:CDS |
| GATGAGGAAGTGGAACATGT+TGG | 0.656984 | 8.4:-19534055 | MS.gene43218:CDS |
| TGAAGGATAATACTGAGTTG+AGG | 0.661198 | 8.4:-19534669 | MS.gene43218:CDS |
| GATGGAATATGGTCAGCTTG+TGG | 0.665052 | 8.4:-19534421 | MS.gene43218:CDS |
| GATCCTGCACTTATAAGGAG+GGG | 0.671043 | 8.4:-19534352 | MS.gene43218:CDS |
| CAAGCTATAAGCAAACTATG+TGG | 0.703587 | 8.4:-19534936 | MS.gene43218:CDS |
| GGAATATGGTCAGCTTGTGG+AGG | 0.710003 | 8.4:-19534418 | MS.gene43218:CDS |
| GTTGATTTACCAGTACCTGG+AGG | 0.741136 | 8.4:+19534754 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTTAATTAAAGAACAAGATG+AGG | - | chr8.4:19535415-19535434 | MS.gene43218:CDS | 20.0% |
| !!! | CTTTCTTATTGTAGTTTTAA+TGG | - | chr8.4:19535179-19535198 | MS.gene43218:CDS | 20.0% |
| ! | AAGTATAGAGATACAATAAC+AGG | - | chr8.4:19534432-19534451 | MS.gene43218:CDS | 25.0% |
| ! | AGAGTAACTGATGATTATAA+AGG | - | chr8.4:19534306-19534325 | MS.gene43218:CDS | 25.0% |
| ! | GAAAGAGATTATTGAAGATT+TGG | - | chr8.4:19534635-19534654 | MS.gene43218:CDS | 25.0% |
| ! | TACAATAACAGGTTCATATT+TGG | - | chr8.4:19534443-19534462 | MS.gene43218:CDS | 25.0% |
| ! | TGAATTTCATTGATGGAATA+TGG | - | chr8.4:19535054-19535073 | MS.gene43218:CDS | 25.0% |
| !! | AAGGATTTTTATGCTAGAAT+TGG | - | chr8.4:19534675-19534694 | MS.gene43218:CDS | 25.0% |
| !! | TGATGATGAGAATGAAAATA+AGG | - | chr8.4:19534950-19534969 | MS.gene43218:CDS | 25.0% |
| !! | TGTTTTCACAACAAATTATG+TGG | - | chr8.4:19535103-19535122 | MS.gene43218:CDS | 25.0% |
| !!! | AAAAGGGGTTATTTACTTTT+TGG | - | chr8.4:19534708-19534727 | MS.gene43218:CDS | 25.0% |
| !!! | GGTTACTTTTAGTAAAAGTA+AGG | - | chr8.4:19534656-19534675 | MS.gene43218:CDS | 25.0% |
| AAAATACAGTCACAGAATCA+TGG | - | chr8.4:19534098-19534117 | MS.gene43218:CDS | 30.0% | |
| AAATTGTATACAAACAGTCC+TGG | - | chr8.4:19534516-19534535 | MS.gene43218:CDS | 30.0% | |
| AATTGGAAAAGCTTGGAAAA+GGG | - | chr8.4:19534692-19534711 | MS.gene43218:CDS | 30.0% | |
| AGCAAGTTTCATGTTCATTT+GGG | - | chr8.4:19534029-19534048 | MS.gene43218:CDS | 30.0% | |
| ATAATCTCTTTCTTCTTCTC+AGG | + | chr8.4:19534627-19534646 | None:intergenic | 30.0% | |
| ATCTCATTCCATGAATTCTT+AGG | - | chr8.4:19534144-19534163 | MS.gene43218:CDS | 30.0% | |
| GGGTTATGATGTTTATGATT+TGG | - | chr8.4:19534782-19534801 | MS.gene43218:CDS | 30.0% | |
| TACTCTTTCATATTCATCCA+TGG | + | chr8.4:19534292-19534311 | None:intergenic | 30.0% | |
| TAGCAAGTTTCATGTTCATT+TGG | - | chr8.4:19534028-19534047 | MS.gene43218:CDS | 30.0% | |
| TGGAATGAGATTCTTATGTA+AGG | + | chr8.4:19534135-19534154 | None:intergenic | 30.0% | |
| TTTCAAAGAAACGTAGAAGT+TGG | + | chr8.4:19534080-19534099 | None:intergenic | 30.0% | |
| ! | TTCTCTTGCTCTTGATAATA+AGG | + | chr8.4:19534384-19534403 | None:intergenic | 30.0% |
| !! | AATGAACATGAAACTTGCTA+AGG | + | chr8.4:19534028-19534047 | None:intergenic | 30.0% |
| !! | ATAAAGGTGTCAAGGTTTAT+TGG | - | chr8.4:19534322-19534341 | MS.gene43218:CDS | 30.0% |
| !! | TAAAGGTGTCAAGGTTTATT+GGG | - | chr8.4:19534323-19534342 | MS.gene43218:CDS | 30.0% |
| !! | TGATGATTATAAAGGTGTCA+AGG | - | chr8.4:19534314-19534333 | MS.gene43218:CDS | 30.0% |
| !!! | AACTTTTGAAACAATGGCTA+TGG | - | chr8.4:19534599-19534618 | MS.gene43218:CDS | 30.0% |
| AAGCAATGAAGCAAATTCAG+AGG | - | chr8.4:19535504-19535523 | MS.gene43218:CDS | 35.0% | |
| AATAGCAAAGTGACACTTTC+AGG | - | chr8.4:19535026-19535045 | MS.gene43218:CDS | 35.0% | |
| ACAGGTCAAAGGAAGAAAAA+AGG | - | chr8.4:19534909-19534928 | MS.gene43218:CDS | 35.0% | |
| ACTGAAATGTGGACAACAAT+GGG | - | chr8.4:19533997-19534016 | MS.gene43218:CDS | 35.0% | |
| ATAGCAAAGTGACACTTTCA+GGG | - | chr8.4:19535027-19535046 | MS.gene43218:CDS | 35.0% | |
| ATCAACAATGATTGCTGCTA+TGG | - | chr8.4:19534749-19534768 | MS.gene43218:CDS | 35.0% | |
| ATCCATGGTTAAAACCAAGT+TGG | + | chr8.4:19534277-19534296 | None:intergenic | 35.0% | |
| ATGCTAGAATTGGAAAAGCT+TGG | - | chr8.4:19534685-19534704 | MS.gene43218:CDS | 35.0% | |
| ATTGGAAAAGCTTGGAAAAG+GGG | - | chr8.4:19534693-19534712 | MS.gene43218:CDS | 35.0% | |
| CAAGCTATAAGCAAACTATG+TGG | - | chr8.4:19534550-19534569 | MS.gene43218:CDS | 35.0% | |
| CCACATTTCAGTCAATTTGT+AGG | + | chr8.4:19533989-19534008 | None:intergenic | 35.0% | |
| CCTACAAATTGACTGAAATG+TGG | - | chr8.4:19533986-19534005 | MS.gene43218:CDS | 35.0% | |
| CTCAAAAGAAGTGATGCTTA+TGG | - | chr8.4:19534174-19534193 | MS.gene43218:CDS | 35.0% | |
| GAATTGGAAAAGCTTGGAAA+AGG | - | chr8.4:19534691-19534710 | MS.gene43218:CDS | 35.0% | |
| GATTGTTCACTTGATCTTAC+AGG | - | chr8.4:19534891-19534910 | MS.gene43218:CDS | 35.0% | |
| GGGCTATTGAATTTCATTGA+TGG | - | chr8.4:19535047-19535066 | MS.gene43218:CDS | 35.0% | |
| GTTGGTCCTATTAAGGAAAA+TGG | - | chr8.4:19535449-19535468 | MS.gene43218:CDS | 35.0% | |
| TAAAGAACAAGATGAGGAAG+TGG | - | chr8.4:19535421-19535440 | MS.gene43218:CDS | 35.0% | |
| TCCTATTAAGGAAAATGGTG+AGG | - | chr8.4:19535454-19535473 | MS.gene43218:CDS | 35.0% | |
| TCTTGCTGATAAAAAAGCTG+AGG | - | chr8.4:19535373-19535392 | MS.gene43218:CDS | 35.0% | |
| TGAAGGATAATACTGAGTTG+AGG | - | chr8.4:19534817-19534836 | MS.gene43218:CDS | 35.0% | |
| TGGATCATGTGATGAAAGAA+GGG | - | chr8.4:19534463-19534482 | MS.gene43218:CDS | 35.0% | |
| TTGGATCATGTGATGAAAGA+AGG | - | chr8.4:19534462-19534481 | MS.gene43218:CDS | 35.0% | |
| ! | ACCTCACCATTTTCCTTAAT+AGG | + | chr8.4:19535458-19535477 | None:intergenic | 35.0% |
| ! | TTGCTCTTGATAATAAGGCA+TGG | + | chr8.4:19534379-19534398 | None:intergenic | 35.0% |
| !! | AAGAAACGTAGAAGTTGGTA+TGG | + | chr8.4:19534075-19534094 | None:intergenic | 35.0% |
| !! | ATTGTTGATTTACCAGTACC+TGG | + | chr8.4:19534738-19534757 | None:intergenic | 35.0% |
| !! | CACATAGTTTGCTTATAGCT+TGG | + | chr8.4:19534552-19534571 | None:intergenic | 35.0% |
| !! | GAGAGTATTGAGAAGCTAAT+TGG | - | chr8.4:19535254-19535273 | MS.gene43218:CDS | 35.0% |
| !! | TAATTTTGATGCTGTGAGGA+AGG | - | chr8.4:19534974-19534993 | MS.gene43218:CDS | 35.0% |
| !!! | TATTTACTTTTTGGTCCTCC+AGG | - | chr8.4:19534717-19534736 | MS.gene43218:CDS | 35.0% |
| !!! | TCCTGCAACTTTTGAAACAA+TGG | - | chr8.4:19534593-19534612 | MS.gene43218:CDS | 35.0% |
| AAGAGACTCAAAGCTGAGAT+TGG | - | chr8.4:19534240-19534259 | MS.gene43218:CDS | 40.0% | |
| AAGGAAAATGGTGAGGTTGT+TGG | - | chr8.4:19535461-19535480 | MS.gene43218:CDS | 40.0% | |
| AAGGGAAAGAGATTCGTTTG+AGG | - | chr8.4:19534481-19534500 | MS.gene43218:CDS | 40.0% | |
| ACTTATAAGGAGGGGTAGAA+TGG | - | chr8.4:19535142-19535161 | MS.gene43218:CDS | 40.0% | |
| AGCTTGATCCTGCACTTATA+AGG | - | chr8.4:19535129-19535148 | MS.gene43218:CDS | 40.0% | |
| CAAACAGTCCTGGTTACAAA+TGG | - | chr8.4:19534526-19534545 | MS.gene43218:CDS | 40.0% | |
| CACTTATCAGCATCATCCAT+TGG | + | chr8.4:19535332-19535351 | None:intergenic | 40.0% | |
| CACTTGATCTTACAGGTCAA+AGG | - | chr8.4:19534898-19534917 | MS.gene43218:CDS | 40.0% | |
| GACTGAAATGTGGACAACAA+TGG | - | chr8.4:19533996-19534015 | MS.gene43218:CDS | 40.0% | |
| GCCATTGTTTCAAAAGTTGC+AGG | + | chr8.4:19534597-19534616 | None:intergenic | 40.0% | |
| GGAACATGTTGGTCCTATTA+AGG | - | chr8.4:19535442-19535461 | MS.gene43218:CDS | 40.0% | |
| GTCTGTCACCTAAGAATTCA+TGG | + | chr8.4:19534155-19534174 | None:intergenic | 40.0% | |
| TCTTATGCCTAAGTCTCCAA+TGG | - | chr8.4:19535313-19535332 | MS.gene43218:CDS | 40.0% | |
| TGATCCTGCACTTATAAGGA+GGG | - | chr8.4:19535133-19535152 | MS.gene43218:CDS | 40.0% | |
| TGCTGATAAAAAAGCTGAGG+AGG | - | chr8.4:19535376-19535395 | MS.gene43218:CDS | 40.0% | |
| TGGAAAAGATAGCACCAACT+TGG | - | chr8.4:19534260-19534279 | MS.gene43218:CDS | 40.0% | |
| TTGATCCTGCACTTATAAGG+AGG | - | chr8.4:19535132-19535151 | MS.gene43218:CDS | 40.0% | |
| ! | TGCTGCTATGGCTAATTTGT+TGG | - | chr8.4:19534761-19534780 | MS.gene43218:CDS | 40.0% |
| ! | TTTGGAGTTAACAGCAGTGA+AGG | - | chr8.4:19534800-19534819 | MS.gene43218:CDS | 40.0% |
| !! | AGGCTAATTTTGATGCTGTG+AGG | - | chr8.4:19534970-19534989 | MS.gene43218:CDS | 40.0% |
| !! | GCTGCTATGGCTAATTTGTT+GGG | - | chr8.4:19534762-19534781 | MS.gene43218:CDS | 40.0% |
| !!! | CACCAACTTGGTTTTAACCA+TGG | - | chr8.4:19534272-19534291 | MS.gene43218:CDS | 40.0% |
| AGATTCTCAGCAACATCAGC+AGG | + | chr8.4:19535296-19535315 | None:intergenic | 45.0% | |
| AGGTGAAGGAAGAAGGAAGT+GGG | - | chr8.4:19534997-19535016 | MS.gene43218:CDS | 45.0% | |
| GAGGAGGTGAAAAAGAGTAG+TGG | - | chr8.4:19535392-19535411 | MS.gene43218:CDS | 45.0% | |
| GATCCTGCACTTATAAGGAG+GGG | - | chr8.4:19535134-19535153 | MS.gene43218:CDS | 45.0% | |
| GATGAGGAAGTGGAACATGT+TGG | - | chr8.4:19535431-19535450 | MS.gene43218:CDS | 45.0% | |
| GATGGAATATGGTCAGCTTG+TGG | - | chr8.4:19535065-19535084 | MS.gene43218:CDS | 45.0% | |
| GCATCATCCATTGGAGACTT+AGG | + | chr8.4:19535323-19535342 | None:intergenic | 45.0% | |
| TAGCTTGGCCATTTGTAACC+AGG | + | chr8.4:19534537-19534556 | None:intergenic | 45.0% | |
| TTCGTTTGAGGAACAGACAG+AGG | - | chr8.4:19534493-19534512 | MS.gene43218:CDS | 45.0% | |
| ! | GTTGATTTACCAGTACCTGG+AGG | + | chr8.4:19534735-19534754 | None:intergenic | 45.0% |
| ! | TTTTGATGCTGTGAGGAAGG+AGG | - | chr8.4:19534977-19534996 | MS.gene43218:CDS | 45.0% |
| AGGAAGGAGGTGAAGGAAGA+AGG | - | chr8.4:19534990-19535009 | MS.gene43218:CDS | 50.0% | |
| CTACCCCTCCTTATAAGTGC+AGG | + | chr8.4:19535140-19535159 | None:intergenic | 50.0% | |
| GAGGTGAAGGAAGAAGGAAG+TGG | - | chr8.4:19534996-19535015 | MS.gene43218:CDS | 50.0% | |
| GGAATATGGTCAGCTTGTGG+AGG | - | chr8.4:19535068-19535087 | MS.gene43218:CDS | 50.0% | |
| GGTGAAGGAAGAAGGAAGTG+GGG | - | chr8.4:19534998-19535017 | MS.gene43218:CDS | 50.0% | |
| GTGAAGGAAGAAGGAAGTGG+GGG | - | chr8.4:19534999-19535018 | MS.gene43218:CDS | 50.0% | |
| !! | CTTTTTGGTCCTCCAGGTAC+TGG | - | chr8.4:19534723-19534742 | MS.gene43218:CDS | 50.0% |
| AAGGAAGAAGGAAGTGGGGG+TGG | - | chr8.4:19535002-19535021 | MS.gene43218:CDS | 55.0% | |
| AGGAAGAAGGAAGTGGGGGT+GGG | - | chr8.4:19535003-19535022 | MS.gene43218:CDS | 55.0% | |
| GGTCAGCTTGTGGAGGTGAA+AGG | - | chr8.4:19535075-19535094 | MS.gene43218:CDS | 55.0% | |
| TGCTGTGAGGAAGGAGGTGA+AGG | - | chr8.4:19534983-19535002 | MS.gene43218:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 19533979 | 19535529 | 19533979 | ID=MS.gene43218 |
| chr8.4 | mRNA | 19533979 | 19535529 | 19533979 | ID=MS.gene43218.t1;Parent=MS.gene43218 |
| chr8.4 | exon | 19533979 | 19535529 | 19533979 | ID=MS.gene43218.t1.exon1;Parent=MS.gene43218.t1 |
| chr8.4 | CDS | 19533979 | 19535529 | 19533979 | ID=cds.MS.gene43218.t1;Parent=MS.gene43218.t1 |
| Gene Sequence |
| Protein sequence |
