AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene43455

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene43455.t1 MTR_4g134280 96.533 375 11 1 123 497 2 374 0.0 744
MS.gene43455.t1 MTR_2g027560 73.407 455 115 3 27 478 21 472 0.0 704
MS.gene43455.t1 MTR_1g010120 52.954 474 212 5 13 484 8 472 3.22e-172 494
MS.gene43455.t1 MTR_4g124440 49.080 489 230 9 19 497 11 490 2.54e-159 461
MS.gene43455.t1 MTR_7g085390 48.049 487 228 8 13 489 6 477 5.26e-159 460
MS.gene43455.t1 MTR_3g108600 51.339 448 190 6 20 465 23 444 5.93e-154 447
MS.gene43455.t1 MTR_6g032820 51.954 435 191 7 16 441 12 437 1.31e-153 445
MS.gene43455.t1 MTR_1g053320 49.438 445 212 6 22 457 16 456 1.37e-148 434
MS.gene43455.t1 MTR_4g112025 46.970 462 235 4 34 489 31 488 3.05e-148 434
MS.gene43455.t1 MTR_8g092070 46.861 446 229 5 30 471 30 471 6.72e-141 417
MS.gene43455.t1 MTR_3g031770 48.492 431 213 4 33 461 26 449 2.26e-140 413
MS.gene43455.t1 MTR_3g095050 44.371 453 239 8 14 459 11 457 1.04e-128 383
MS.gene43455.t1 MTR_5g085580 33.189 461 258 15 34 460 25 469 5.81e-66 220
MS.gene43455.t1 MTR_6g088885 31.613 465 292 13 5 458 6 455 6.41e-66 221
MS.gene43455.t1 MTR_1g056640 32.976 467 264 17 33 460 37 493 3.46e-64 216
MS.gene43455.t1 MTR_4g088155 34.667 450 257 20 32 457 21 457 4.36e-64 215
MS.gene43455.t1 MTR_4g069940 32.385 457 268 17 30 462 25 464 1.70e-61 208
MS.gene43455.t1 MTR_8g085720 33.105 438 264 16 34 457 26 448 3.12e-61 208
MS.gene43455.t1 MTR_8g012400 32.574 439 270 15 35 457 30 458 7.19e-61 207
MS.gene43455.t1 MTR_3g116510 33.259 448 272 16 30 462 24 459 2.96e-60 205
MS.gene43455.t1 MTR_1g031900 32.026 459 284 15 12 457 2 445 3.61e-57 197
MS.gene43455.t1 MTR_1g031900 32.026 459 284 15 12 457 2 445 5.09e-57 197
MS.gene43455.t1 MTR_3g080410 37.048 332 194 10 26 349 17 341 1.72e-55 189
MS.gene43455.t1 MTR_4g076570 34.259 324 202 7 30 351 18 332 2.89e-55 189
MS.gene43455.t1 MTR_7g053500 31.448 442 274 16 35 457 27 458 2.02e-54 189
MS.gene43455.t1 MTR_3g083580 29.936 471 296 17 14 471 7 456 8.39e-54 189
MS.gene43455.t1 MTR_4g132280 30.064 469 287 15 34 480 45 494 2.27e-53 188
MS.gene43455.t1 MTR_1g052430 34.483 319 196 9 34 349 32 340 1.04e-51 181
MS.gene43455.t1 MTR_7g081350 33.918 342 211 9 13 351 6 335 1.70e-51 178
MS.gene43455.t1 MTR_2g034470 33.231 325 206 6 29 351 25 340 2.76e-51 178
MS.gene43455.t1 MTR_2g034440 33.436 326 205 7 29 351 17 333 3.59e-51 177
MS.gene43455.t1 MTR_8g102340 31.981 419 252 16 92 487 50 458 3.66e-51 181
MS.gene43455.t1 MTR_2g034480 32.764 351 221 8 7 354 2 340 1.78e-50 176
MS.gene43455.t1 MTR_3g065460 31.081 444 270 16 31 457 21 445 2.42e-50 179
MS.gene43455.t1 MTR_4g076255 35.276 326 187 11 33 353 24 330 4.23e-50 174
MS.gene43455.t1 MTR_4g083500 30.261 499 290 19 34 489 44 527 4.56e-50 179
MS.gene43455.t1 MTR_7g026340 29.778 450 280 19 26 456 15 447 7.84e-50 177
MS.gene43455.t1 MTR_2g034470 33.775 302 189 6 52 351 13 305 4.06e-49 172
MS.gene43455.t1 MTR_8g012400 31.783 387 238 15 87 457 2 378 3.75e-48 171
MS.gene43455.t1 MTR_5g078200 29.322 457 295 14 14 457 3 444 7.42e-48 175
MS.gene43455.t1 MTR_4g010200 34.877 324 193 10 35 349 34 348 1.32e-47 169
MS.gene43455.t1 MTR_7g053500 32.564 390 234 16 87 457 2 381 1.43e-47 169
MS.gene43455.t1 MTR_4g084480 32.931 331 207 11 32 356 30 351 1.20e-46 167
MS.gene43455.t1 MTR_4g084480 32.931 331 207 11 32 356 30 351 1.25e-46 167
MS.gene43455.t1 MTR_4g084480 32.931 331 207 11 32 356 23 344 1.64e-46 166
MS.gene43455.t1 MTR_1g022160 28.541 466 282 16 34 460 24 477 1.73e-46 169
MS.gene43455.t1 MTR_1g022160 28.726 463 279 16 34 457 24 474 3.58e-46 168
MS.gene43455.t1 MTR_7g081370 33.432 338 198 10 16 351 11 323 1.13e-45 162
MS.gene43455.t1 MTR_5g078200 29.481 424 271 14 47 457 3 411 2.13e-45 168
MS.gene43455.t1 MTR_4g052970 33.425 365 212 15 33 389 28 369 2.37e-45 163
MS.gene43455.t1 MTR_1g022160 28.509 456 275 16 34 450 24 467 1.90e-44 163
MS.gene43455.t1 MTR_4g076470 31.395 344 212 12 10 349 1 324 2.28e-44 159
MS.gene43455.t1 MTR_4g114850 31.742 356 220 11 35 376 27 373 2.30e-44 160
MS.gene43455.t1 MTR_4g114850 32.716 324 200 10 35 349 27 341 3.82e-44 160
MS.gene43455.t1 MTR_1g077990 32.727 330 206 11 33 355 30 350 4.99e-44 160
MS.gene43455.t1 MTR_4g114850 32.716 324 200 10 35 349 27 341 6.00e-44 159
MS.gene43455.t1 MTR_4g076430 30.460 348 218 12 10 353 1 328 2.09e-43 156
MS.gene43455.t1 MTR_4g076500 32.166 314 194 9 33 341 77 376 4.46e-43 157
MS.gene43455.t1 MTR_3g116510 34.132 334 196 14 144 462 1 325 1.60e-42 154
MS.gene43455.t1 MTR_4g076440 30.058 346 218 12 10 351 1 326 2.19e-42 154
MS.gene43455.t1 MTR_4g076490 30.994 342 213 12 29 366 92 414 3.18e-42 155
MS.gene43455.t1 MTR_1g007810 33.861 316 190 12 34 341 16 320 1.22e-41 152
MS.gene43455.t1 MTR_2g086530 30.028 363 225 15 35 388 30 372 1.99e-40 150
MS.gene43455.t1 MTR_2g086530 30.028 363 225 15 35 388 9 351 2.44e-40 149
MS.gene43455.t1 MTR_2g019345 31.707 328 206 12 35 355 25 341 9.47e-40 148
MS.gene43455.t1 MTR_1g022160 31.481 324 207 9 34 349 24 340 4.20e-39 147
MS.gene43455.t1 MTR_5g081720 30.592 304 181 13 59 349 62 348 4.23e-30 121
MS.gene43455.t1 MTR_8g085440 44.792 96 47 4 365 457 23 115 2.16e-15 72.8
MS.gene43455.t1 MTR_4g036680 36.364 99 58 3 253 351 82 175 1.06e-14 73.6
MS.gene43455.t1 MTR_4g036775 36.000 100 59 3 253 352 82 176 3.35e-14 72.4
MS.gene43455.t1 MTR_8g085420 42.105 95 49 4 365 456 23 114 1.91e-12 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene43455.t1 AT4G17180 71.982 439 115 2 31 469 22 452 0.0 670
MS.gene43455.t1 AT4G31140 49.275 483 235 6 9 488 5 480 5.53e-165 476
MS.gene43455.t1 AT5G58090 51.522 460 213 5 32 488 20 472 1.38e-157 456
MS.gene43455.t1 AT3G24330 48.214 448 221 7 32 472 37 480 8.18e-152 442
MS.gene43455.t1 AT1G64760 48.908 458 222 7 10 461 1 452 1.52e-151 441
MS.gene43455.t1 AT1G64760 48.908 458 222 7 10 461 1 452 1.52e-151 441
MS.gene43455.t1 AT2G19440 47.737 486 236 9 12 488 2 478 2.76e-151 441
MS.gene43455.t1 AT2G19440 47.737 486 236 9 12 488 2 478 2.76e-151 441
MS.gene43455.t1 AT5G20870 49.460 463 207 10 34 476 30 485 5.80e-146 428
MS.gene43455.t1 AT5G18220 45.940 468 231 9 32 491 28 481 8.29e-144 422
MS.gene43455.t1 AT3G04010 46.259 441 226 7 32 466 31 466 2.04e-142 418
MS.gene43455.t1 AT5G64790 44.493 454 233 6 12 461 17 455 1.72e-133 395
MS.gene43455.t1 AT5G58480 45.575 452 234 7 30 478 22 464 4.95e-131 389
MS.gene43455.t1 AT5G55180 35.440 443 260 15 34 460 27 459 1.79e-71 234
MS.gene43455.t1 AT2G05790 34.565 460 257 15 33 460 24 471 7.00e-71 233
MS.gene43455.t1 AT5G55180 35.484 434 254 15 34 451 27 450 1.57e-68 227
MS.gene43455.t1 AT4G26830 32.812 448 262 15 34 460 29 458 4.11e-65 218
MS.gene43455.t1 AT4G26830 32.812 448 262 15 34 460 25 454 5.33e-65 217
MS.gene43455.t1 AT3G07320 34.921 441 258 18 35 457 26 455 8.28e-62 209
MS.gene43455.t1 AT2G01630 32.045 440 271 14 31 457 20 444 2.81e-60 206
MS.gene43455.t1 AT5G24318 30.603 464 284 19 14 457 10 455 1.13e-58 201
MS.gene43455.t1 AT2G01630 31.724 435 269 14 31 452 20 439 1.22e-58 200
MS.gene43455.t1 AT3G13560 31.991 447 275 15 34 467 26 456 2.74e-58 201
MS.gene43455.t1 AT3G13560 31.991 447 275 15 34 467 26 456 2.74e-58 201
MS.gene43455.t1 AT3G13560 31.991 447 275 15 34 467 26 456 2.74e-58 201
MS.gene43455.t1 AT3G13560 31.991 447 275 15 34 467 26 456 2.74e-58 201
MS.gene43455.t1 AT3G13560 31.991 447 275 15 34 467 26 456 2.74e-58 201
MS.gene43455.t1 AT5G56590 29.764 467 291 15 23 470 20 468 1.06e-57 199
MS.gene43455.t1 AT3G23770 33.185 449 265 18 32 457 35 471 1.30e-56 196
MS.gene43455.t1 AT5G24318 30.501 459 281 19 14 452 10 450 2.91e-56 194
MS.gene43455.t1 AT4G29360 29.730 481 290 14 32 475 35 504 1.11e-55 195
MS.gene43455.t1 AT4G29360 29.730 481 290 14 32 475 25 494 1.28e-55 194
MS.gene43455.t1 AT4G34480 32.533 458 283 15 12 457 4 447 4.38e-55 192
MS.gene43455.t1 AT1G11820 29.607 483 298 16 34 489 44 511 6.53e-55 192
MS.gene43455.t1 AT3G55430 32.653 441 264 21 32 457 27 449 6.85e-55 191
MS.gene43455.t1 AT2G16230 30.816 490 302 18 27 493 18 493 1.02e-54 191
MS.gene43455.t1 AT4G14080 31.839 446 272 17 32 457 40 473 3.24e-53 187
MS.gene43455.t1 AT3G57270 34.356 326 203 7 30 353 23 339 4.12e-53 182
MS.gene43455.t1 AT1G11820 30.394 431 272 14 34 451 44 459 2.08e-52 184
MS.gene43455.t1 AT1G66250 29.560 477 298 14 27 480 25 486 3.63e-52 184
MS.gene43455.t1 AT3G57260 33.846 325 200 8 30 353 28 338 4.19e-52 180
MS.gene43455.t1 AT3G57260 33.846 325 200 8 30 353 44 354 5.93e-52 180
MS.gene43455.t1 AT4G16260 32.849 344 217 9 10 351 1 332 2.34e-49 173
MS.gene43455.t1 AT4G18340 31.250 368 233 12 11 368 10 367 1.75e-48 172
MS.gene43455.t1 AT4G18340 31.250 368 233 12 11 368 10 367 4.62e-48 171
MS.gene43455.t1 AT4G16260 34.098 305 188 8 49 351 10 303 2.01e-47 167
MS.gene43455.t1 AT2G39640 31.798 456 267 22 16 455 13 440 2.23e-47 172
MS.gene43455.t1 AT2G01630 32.335 334 208 10 31 354 20 345 7.34e-47 167
MS.gene43455.t1 AT5G42720 33.428 353 216 12 11 353 3 346 3.81e-46 167
MS.gene43455.t1 AT3G15800 34.146 328 199 10 35 353 44 363 4.32e-46 166
MS.gene43455.t1 AT4G34480 35.043 351 212 10 12 354 4 346 7.79e-46 164
MS.gene43455.t1 AT1G30080 33.030 330 196 12 32 349 34 350 2.97e-45 164
MS.gene43455.t1 AT1G30080 33.030 330 196 12 32 349 34 350 3.05e-45 163
MS.gene43455.t1 AT3G57240 32.609 322 199 9 34 353 35 340 2.36e-44 159
MS.gene43455.t1 AT2G26600 31.928 332 206 11 35 357 35 355 3.87e-44 160
MS.gene43455.t1 AT5G24318 31.215 362 231 13 14 368 10 360 3.90e-44 159
MS.gene43455.t1 AT2G27500 29.781 366 229 12 34 392 29 373 5.40e-44 159
MS.gene43455.t1 AT2G27500 30.387 362 224 12 34 388 29 369 1.31e-43 159
MS.gene43455.t1 AT1G32860 32.143 336 207 12 33 357 28 353 6.03e-42 155
MS.gene43455.t1 AT5G24318 32.622 328 188 15 149 457 3 316 2.22e-40 148
MS.gene43455.t1 AT1G33220 33.115 305 189 8 28 327 28 322 9.92e-40 147
MS.gene43455.t1 AT1G11820 30.000 330 215 9 34 354 44 366 5.86e-39 146
MS.gene43455.t1 AT3G61810 28.125 384 229 12 1 354 6 372 6.17e-39 145
MS.gene43455.t1 AT5G20390 31.949 313 195 9 34 341 33 332 9.40e-39 144
MS.gene43455.t1 AT1G77790 31.715 309 195 9 48 351 44 341 1.28e-38 144
MS.gene43455.t1 AT5G20340 33.016 315 193 9 32 341 41 342 2.04e-38 144
MS.gene43455.t1 AT5G42100 32.239 335 209 12 29 353 22 348 3.95e-38 144
MS.gene43455.t1 AT5G42100 32.239 335 209 12 29 353 22 348 4.26e-38 144
MS.gene43455.t1 AT1G77780 29.730 296 195 6 48 341 38 322 6.99e-38 142
MS.gene43455.t1 AT5G20560 30.794 315 205 8 30 341 30 334 2.19e-37 140
MS.gene43455.t1 AT4G34480 32.659 346 209 14 124 457 3 336 3.75e-37 141
MS.gene43455.t1 AT5G20330 31.348 319 201 9 28 341 28 333 5.07e-37 139
MS.gene43455.t1 AT2G27500 30.128 312 190 12 84 388 1 291 3.02e-34 131
MS.gene43455.t1 AT3G46570 31.389 360 221 14 11 358 4 349 3.09e-34 132
MS.gene43455.t1 AT2G26600 31.250 272 167 11 95 357 1 261 6.60e-31 121
MS.gene43455.t1 AT3G55780 24.332 337 232 10 39 366 43 365 1.07e-28 117

Find 125 sgRNAs with CRISPR-Local

Find 174 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTATAGTTATTGGAGAAAT+TGG 0.161486 8.4:+508187 MS.gene43455:CDS
GGGTAGATATTAACAATAAA+TGG 0.180808 8.4:-508001 None:intergenic
CCCCTTCTGCTTCTGATTTG+TGG 0.212209 8.4:+506825 MS.gene43455:CDS
GGTCAAATTCCTATAGTTAT+TGG 0.217381 8.4:+508178 MS.gene43455:CDS
TTTCCAAGGCCAAGGTTTAA+TGG 0.241450 8.4:-508436 None:intergenic
GTCGCAGCACTCAGTAAAAT+TGG 0.243518 8.4:+508151 MS.gene43455:CDS
ATTTATAGCAATGCATTTGA+TGG 0.264940 8.4:+508112 MS.gene43455:CDS
GAACTAGCTGAGTCATGATT+TGG 0.293607 8.4:-507955 None:intergenic
TTCAGTATCTTCCGTCTAAA+TGG 0.295863 8.4:+508485 MS.gene43455:CDS
GATTGTACAACTCTTGATTA+TGG 0.306971 8.4:+508586 MS.gene43455:CDS
GGAGGTTCATGCAATGGAAT+TGG 0.323892 8.4:+508607 MS.gene43455:CDS
CCTTCTCTAAGGATTGTTGT+AGG 0.325174 8.4:-507835 None:intergenic
GGTCTTATCAACCATGTTCT+TGG 0.327129 8.4:+508268 MS.gene43455:CDS
AGCCAGCTTTGCCTTCTCTA+AGG 0.335120 8.4:-507846 None:intergenic
TCAACTGGTGGATCTCAAAT+AGG 0.335178 8.4:+508790 MS.gene43455:CDS
CGGTGTAAGCAGAGACGTTC+TGG 0.340269 8.4:-506853 None:intergenic
GGTAGATATTAACAATAAAT+GGG 0.351539 8.4:-508000 None:intergenic
CCAATTTCTCCAATAACTAT+AGG 0.363185 8.4:-508187 None:intergenic
GAGGTTCATGCAATGGAATT+GGG 0.364355 8.4:+508608 MS.gene43455:CDS
AATCAGCCATAGGAGTAAAC+TGG 0.364459 8.4:+506624 MS.gene43455:CDS
GATATCAGCACCACCTTTCC+CGG 0.368497 8.4:-506873 None:intergenic
GGAAAGGTGGTGCTGATATC+AGG 0.370412 8.4:+506876 MS.gene43455:CDS
GCACTCAGTAAAATTGGATA+TGG 0.381123 8.4:+508157 MS.gene43455:CDS
CAAGACTATGCGTTCTTTGA+GGG 0.405455 8.4:+508058 MS.gene43455:CDS
CAAAAGAAACAACATCTCAT+TGG 0.414587 8.4:-506795 None:intergenic
TTTGACATTAATAATGATGT+TGG 0.417994 8.4:+506571 MS.gene43455:CDS
GTCGCCGACTGAAGGGTCTC+TGG 0.419124 8.4:-508728 None:intergenic
AGCAGAGGCTGATGTTCTTA+AGG 0.419618 8.4:+506733 MS.gene43455:CDS
TATCCATTAAACCTTGGCCT+TGG 0.421251 8.4:+508433 MS.gene43455:CDS
ACAGAGCCTTGCAACAGGTT+TGG 0.424953 8.4:+506536 MS.gene43455:CDS
TGATGTTGGGTTTATGCTGC+TGG 0.429224 8.4:+506585 MS.gene43455:CDS
GTGGAATTCAGGTGATGGTT+GGG 0.429711 8.4:+506768 MS.gene43455:CDS
CCATTTCTTAAAAGTTATAA+CGG 0.439246 8.4:+507776 MS.gene43455:CDS
TCTTCAATTCCTTGTTTCCA+AGG 0.445933 8.4:-508450 None:intergenic
CAGTCGAAGGCTTCAATTTG+CGG 0.446370 8.4:-506661 None:intergenic
AACTAGCTGAGTCATGATTT+GGG 0.447469 8.4:-507954 None:intergenic
ATCCTTAGAGAAGGCAAAGC+TGG 0.449396 8.4:+507844 MS.gene43455:CDS
ACAAGACTATGCGTTCTTTG+AGG 0.449531 8.4:+508057 MS.gene43455:CDS
ATATCCATAGGAGGAGCATT+AGG 0.450841 8.4:-508313 None:intergenic
TGGCCTTCAGATGGAGCAAT+TGG 0.450952 8.4:+508211 MS.gene43455:CDS
TTGACATTAATAATGATGTT+GGG 0.450983 8.4:+506572 MS.gene43455:CDS
AATTACAGGTATGTTGCTGT+TGG 0.453794 8.4:+507746 MS.gene43455:intron
AAAAGAAACAACATCTCATT+GGG 0.456722 8.4:-506794 None:intergenic
CGTGCCCCAGTTTACTCCTA+TGG 0.462495 8.4:-506630 None:intergenic
GGAGCAAAGAGTATTCTTCC+AGG 0.464462 8.4:+508361 MS.gene43455:CDS
CAAAGAACGCATAGTCTTGT+GGG 0.465598 8.4:-508054 None:intergenic
ACTCATGGGAAGTGGAATTC+AGG 0.466332 8.4:+506757 MS.gene43455:CDS
CTGTTCAGCCTTCTTGATGA+AGG 0.466581 8.4:+508340 MS.gene43455:CDS
GTAACAAGGGGACACCTTTG+AGG 0.473169 8.4:+508290 MS.gene43455:CDS
GAGGCCTAATGCTCCTCCTA+TGG 0.474425 8.4:+508309 MS.gene43455:CDS
ATGGCTGATTCTGTCAATAC+AGG 0.476202 8.4:-506611 None:intergenic
CAAATTGAAGCCTTCGACTG+TGG 0.484496 8.4:+506664 MS.gene43455:CDS
GATAAAGCTACACGTTCAAC+TGG 0.485931 8.4:+508775 MS.gene43455:CDS
AGTGGAATTCAGGTGATGGT+TGG 0.486153 8.4:+506767 MS.gene43455:CDS
GTCATGATTTGGGTTAGATC+AGG 0.487046 8.4:-507944 None:intergenic
CAACCATGTTCTTGGTAACA+AGG 0.487104 8.4:+508276 MS.gene43455:CDS
TAATGACAGAGCCTTGCAAC+AGG 0.487466 8.4:+506531 None:intergenic
TGATGTTCTTAAGGCACTCA+TGG 0.496691 8.4:+506742 MS.gene43455:CDS
TGTTGTAGGTTGAGTACTGC+AGG 0.501811 8.4:-507821 None:intergenic
TATCCCTTGTGAAGGAATTG+AGG 0.502246 8.4:-507915 None:intergenic
CAATGTCTTTCAAAACTACC+TGG 0.504549 8.4:-508379 None:intergenic
ATTCCTTGTTTCCAAGGCCA+AGG 0.514113 8.4:-508444 None:intergenic
AAACCTTGGCCTTGGAAACA+AGG 0.515265 8.4:+508441 MS.gene43455:CDS
AAACGACAGTCGCCGACTGA+AGG 0.516511 8.4:-508736 None:intergenic
CTCAAATAGGATATAGTTGG+TGG 0.517574 8.4:+508803 MS.gene43455:CDS
GCCTTGCAACAGGTTTGGTA+TGG 0.517906 8.4:+506541 MS.gene43455:CDS
GCACCAATTGCTCCATCTGA+AGG 0.518393 8.4:-508214 None:intergenic
GAAATTGGTTGGCCTTCAGA+TGG 0.520140 8.4:+508202 MS.gene43455:CDS
TAGTTATTGGAGAAATTGGT+TGG 0.521897 8.4:+508191 MS.gene43455:CDS
CGTCGTAGAGGCTGAGGAAA+GGG 0.522100 8.4:-508021 None:intergenic
GTTCAGTTCCTCAACTCAAA+CGG 0.528913 8.4:+507974 MS.gene43455:CDS
GATCTCAAATAGGATATAGT+TGG 0.530437 8.4:+508800 MS.gene43455:CDS
ATGGGGAACCGTTTGAGTTG+AGG 0.536855 8.4:-507982 None:intergenic
TGAGTACTGCAGGCATGACC+AGG 0.540544 8.4:-507811 None:intergenic
AACGACAGTCGCCGACTGAA+GGG 0.543117 8.4:-508735 None:intergenic
ATCAGCCATAGGAGTAAACT+GGG 0.543941 8.4:+506625 MS.gene43455:CDS
CTTAAGGCACTCATGGGAAG+TGG 0.544463 8.4:+506749 MS.gene43455:CDS
GTCTCTGCTTACACCGGGAA+AGG 0.548221 8.4:+506860 MS.gene43455:CDS
TAACGGTCAATTTCAGAACC+TGG 0.548760 8.4:+507793 MS.gene43455:CDS
ATATTTGAACCATCTGTGAC+AGG 0.551387 8.4:-508091 None:intergenic
GCTAAGTATCCATTAAACCT+TGG 0.553117 8.4:+508427 MS.gene43455:CDS
TCAAAGAACGCATAGTCTTG+TGG 0.554316 8.4:-508055 None:intergenic
TCTTTGCTCCTTCATCAAGA+AGG 0.555297 8.4:-508348 None:intergenic
AGTGAAACTGTTTGAAGCAG+AGG 0.555786 8.4:+506718 MS.gene43455:CDS
CAGTATCGTCGTAGAGGCTG+AGG 0.558216 8.4:-508027 None:intergenic
AACCATGTTCTTGGTAACAA+GGG 0.564205 8.4:+508277 MS.gene43455:CDS
CTGAACAGATAGATATCCAT+AGG 0.564300 8.4:-508325 None:intergenic
GATTATGGAGGTTCATGCAA+TGG 0.567791 8.4:+508601 MS.gene43455:CDS
TCATAGACATCTGCATTGCA+TGG 0.573085 8.4:-507890 None:intergenic
TCCACAAATCAGAAGCAGAA+GGG 0.574897 8.4:-506826 None:intergenic
CCACAAATCAGAAGCAGAAG+GGG 0.577752 8.4:-506825 None:intergenic
TGCAATGGAATTGGGGACAA+AGG 0.581526 8.4:+508616 MS.gene43455:CDS
GGAGGAGCATTAGGCCTCAA+AGG 0.591534 8.4:-508304 None:intergenic
ATCCACAAATCAGAAGCAGA+AGG 0.594435 8.4:-506827 None:intergenic
TCCCCTTGTTACCAAGAACA+TGG 0.599301 8.4:-508279 None:intergenic
CTTACCACACACCATTTAGA+CGG 0.600170 8.4:-508496 None:intergenic
AAAGCTACACGTTCAACTGG+TGG 0.601725 8.4:+508778 MS.gene43455:CDS
TCGTCGTAGAGGCTGAGGAA+AGG 0.602157 8.4:-508022 None:intergenic
TGTACAACTCTTGATTATGG+AGG 0.604592 8.4:+508589 MS.gene43455:CDS
AGGTTCATGCAATGGAATTG+GGG 0.605748 8.4:+508609 MS.gene43455:CDS
TGGTTTGAGTATTAATAATG+TGG 0.606241 8.4:+508531 MS.gene43455:CDS
GATGTTCTTAAGGCACTCAT+GGG 0.608418 8.4:+506743 MS.gene43455:CDS
TCTGCTTACACCGGGAAAGG+TGG 0.610018 8.4:+506863 MS.gene43455:CDS
AAGAGATCAACCACAGTCGA+AGG 0.614973 8.4:-506674 None:intergenic
ACTACTCATCCTGTCACAGA+TGG 0.616505 8.4:+508082 MS.gene43455:CDS
GCCATACCAAACCTGTTGCA+AGG 0.618952 8.4:-506542 None:intergenic
CAAATCAGAAGCAGAAGGGG+AGG 0.619027 8.4:-506822 None:intergenic
GTGGTAAGTTCTAAGTCAAG+TGG 0.623574 8.4:+508511 MS.gene43455:CDS
CCTACAACAATCCTTAGAGA+AGG 0.625958 8.4:+507835 MS.gene43455:CDS
GGACGGAATATCCCTTGTGA+AGG 0.626622 8.4:-507923 None:intergenic
ACCATGTTCTTGGTAACAAG+GGG 0.635252 8.4:+508278 MS.gene43455:CDS
AACAGATAGATATCCATAGG+AGG 0.636789 8.4:-508322 None:intergenic
CAGAACGTCTCTGCTTACAC+CGG 0.637608 8.4:+506854 MS.gene43455:CDS
ACTTCCAGAGACCCTTCAGT+CGG 0.638338 8.4:+508724 MS.gene43455:CDS
GAATCCTCAATTCCTTCACA+AGG 0.640274 8.4:+507911 MS.gene43455:CDS
AGAACGTCTCTGCTTACACC+GGG 0.644702 8.4:+506855 MS.gene43455:CDS
CATAGGAGTAAACTGGGGCA+CGG 0.648386 8.4:+506631 MS.gene43455:CDS
GGAAATCAGTATCGTCGTAG+AGG 0.649323 8.4:-508033 None:intergenic
GTAGATATTAACAATAAATG+GGG 0.652349 8.4:-507999 None:intergenic
TGATTTGGGTTAGATCAGGA+CGG 0.652522 8.4:-507940 None:intergenic
GTATTGACAGAATCAGCCAT+AGG 0.655460 8.4:+506614 MS.gene43455:CDS
AATCCTCAATTCCTTCACAA+GGG 0.658418 8.4:+507912 MS.gene43455:CDS
GGGAAGTGGAATTCAGGTGA+TGG 0.670388 8.4:+506763 MS.gene43455:CDS
TCTTCCGTCTAAATGGTGTG+TGG 0.709729 8.4:+508492 MS.gene43455:CDS
TCAGCCATAGGAGTAAACTG+GGG 0.815969 8.4:+506626 MS.gene43455:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATCTTTTACAACAAATAATA+AGG + chr8.4:507061-507080 MS.gene43455:intron 15.0%
!! CATAACAAAAATTAGATTCT+TGG - chr8.4:507222-507241 None:intergenic 20.0%
!! CCATTTCTTAAAAGTTATAA+CGG + chr8.4:507776-507795 MS.gene43455:CDS 20.0%
!! GGTAGATATTAACAATAAAT+GGG - chr8.4:508003-508022 None:intergenic 20.0%
!! GTAGATATTAACAATAAATG+GGG - chr8.4:508002-508021 None:intergenic 20.0%
!! TCCAAAAAATAAAATGCAAA+AGG - chr8.4:507630-507649 None:intergenic 20.0%
!! TTGACATTAATAATGATGTT+GGG + chr8.4:506572-506591 MS.gene43455:CDS 20.0%
!! TTTGAACTAACAAATTTACT+AGG + chr8.4:507518-507537 MS.gene43455:intron 20.0%
!! TTTGACATTAATAATGATGT+TGG + chr8.4:506571-506590 MS.gene43455:CDS 20.0%
!!! CTAATTTTTGTTATGTTTAG+TGG + chr8.4:507227-507246 MS.gene43455:intron 20.0%
!!! TTGATAATAATTTGAGAAAC+TGG + chr8.4:507337-507356 MS.gene43455:intron 20.0%
!!! TTGTCAATTATTTTAAGTCT+TGG + chr8.4:507587-507606 MS.gene43455:intron 20.0%
! AAAAGAAACAACATCTCATT+GGG - chr8.4:506797-506816 None:intergenic 25.0%
! AATATCAAGTATTCATGTGA+AGG + chr8.4:507131-507150 MS.gene43455:intron 25.0%
! ACTTAAATAACTAGAGTTTC+TGG + chr8.4:507670-507689 MS.gene43455:intron 25.0%
! ATTTATAGCAATGCATTTGA+TGG + chr8.4:508112-508131 MS.gene43455:CDS 25.0%
! CTCTAGTTATTTAAGTATCA+AGG - chr8.4:507666-507685 None:intergenic 25.0%
! GGGTAGATATTAACAATAAA+TGG - chr8.4:508004-508023 None:intergenic 25.0%
! GTAATTTAAAAAGACCTCTA+AGG - chr8.4:507732-507751 None:intergenic 25.0%
! TGGTTTGAGTATTAATAATG+TGG + chr8.4:508531-508550 MS.gene43455:CDS 25.0%
!! TAGAGGTCTTTTTAAATTAC+AGG + chr8.4:507732-507751 MS.gene43455:intron 25.0%
!!! ATAGTTATTGGTTTTGAAAG+AGG - chr8.4:506972-506991 None:intergenic 25.0%
!!! CCGTTATAACTTTTAAGAAA+TGG - chr8.4:507779-507798 None:intergenic 25.0%
!!! GCCTTTTGCATTTTATTTTT+TGG + chr8.4:507626-507645 MS.gene43455:intron 25.0%
CAAAAGAAACAACATCTCAT+TGG - chr8.4:506798-506817 None:intergenic 30.0%
CCAATTTCTCCAATAACTAT+AGG - chr8.4:508190-508209 None:intergenic 30.0%
CCTATAGTTATTGGAGAAAT+TGG + chr8.4:508187-508206 MS.gene43455:CDS 30.0%
GAAACTAGGCTTATAGTTAT+TGG - chr8.4:506984-507003 None:intergenic 30.0%
GATCTCAAATAGGATATAGT+TGG + chr8.4:508800-508819 MS.gene43455:CDS 30.0%
GATTGTACAACTCTTGATTA+TGG + chr8.4:508586-508605 MS.gene43455:CDS 30.0%
GGTCAAATTCCTATAGTTAT+TGG + chr8.4:508178-508197 MS.gene43455:CDS 30.0%
TACAAGTCTTTAAACCTTAG+AGG + chr8.4:507715-507734 MS.gene43455:intron 30.0%
TAGTTATTGGAGAAATTGGT+TGG + chr8.4:508191-508210 MS.gene43455:CDS 30.0%
TGGAATAACTGAATACAACT+AGG + chr8.4:507357-507376 MS.gene43455:intron 30.0%
TTCAAAATCGTTCAGATGTA+TGG - chr8.4:507006-507025 None:intergenic 30.0%
! CTCGAAATTTCATGTCTTTT+TGG + chr8.4:507253-507272 MS.gene43455:intron 30.0%
!! GAACTAACACTTTTGATTGA+AGG - chr8.4:507394-507413 None:intergenic 30.0%
!!! AGGTAGTTTTGAAAGACATT+GGG + chr8.4:508381-508400 MS.gene43455:CDS 30.0%
AACAGATAGATATCCATAGG+AGG - chr8.4:508325-508344 None:intergenic 35.0%
AACTAGCTGAGTCATGATTT+GGG - chr8.4:507957-507976 None:intergenic 35.0%
AATCCTCAATTCCTTCACAA+GGG + chr8.4:507912-507931 MS.gene43455:CDS 35.0%
AATTACAGGTATGTTGCTGT+TGG + chr8.4:507746-507765 MS.gene43455:intron 35.0%
AGAATGCAAAAGAGTTCAGT+TGG - chr8.4:507490-507509 None:intergenic 35.0%
AGCCCAAGTAGTCATATATA+TGG - chr8.4:507544-507563 None:intergenic 35.0%
AGGCCATATATATGACTACT+TGG + chr8.4:507538-507557 MS.gene43455:intron 35.0%
ATATTTGAACCATCTGTGAC+AGG - chr8.4:508094-508113 None:intergenic 35.0%
CAATGTCTTTCAAAACTACC+TGG - chr8.4:508382-508401 None:intergenic 35.0%
CTCAAATAGGATATAGTTGG+TGG + chr8.4:508803-508822 MS.gene43455:CDS 35.0%
CTGAACAGATAGATATCCAT+AGG - chr8.4:508328-508347 None:intergenic 35.0%
GATCTATGAAACCCTTAACA+TGG + chr8.4:507285-507304 MS.gene43455:intron 35.0%
GCACTCAGTAAAATTGGATA+TGG + chr8.4:508157-508176 MS.gene43455:CDS 35.0%
GCTAAGTATCCATTAAACCT+TGG + chr8.4:508427-508446 MS.gene43455:CDS 35.0%
GCTGATCTTCTGATTATTAC+TGG + chr8.4:507181-507200 MS.gene43455:intron 35.0%
GGCCATATATATGACTACTT+GGG + chr8.4:507539-507558 MS.gene43455:intron 35.0%
TAAACTTTCAGAACAGTTGC+AGG - chr8.4:507032-507051 None:intergenic 35.0%
TGTACAACTCTTGATTATGG+AGG + chr8.4:508589-508608 MS.gene43455:CDS 35.0%
TTCAGTATCTTCCGTCTAAA+TGG + chr8.4:508485-508504 MS.gene43455:CDS 35.0%
! AACCATGTTCTTGGTAACAA+GGG + chr8.4:508277-508296 MS.gene43455:CDS 35.0%
! GAACTTTTCCTTTTGAACTC+TGG + chr8.4:507153-507172 MS.gene43455:intron 35.0%
! GCAGCTAAAGTTTTCAATCA+AGG + chr8.4:508247-508266 MS.gene43455:CDS 35.0%
! TCAAGAAGCTGCAATTTTGA+TGG + chr8.4:508688-508707 MS.gene43455:CDS 35.0%
! TCTTCAATTCCTTGTTTCCA+AGG - chr8.4:508453-508472 None:intergenic 35.0%
! TGACTACTTGGGCTTTTAAT+TGG + chr8.4:507550-507569 MS.gene43455:intron 35.0%
! TGGGGGATATTTTACTTTGA+TGG + chr8.4:508400-508419 MS.gene43455:CDS 35.0%
!!! CAATTTTGATGGACTTGGTA+TGG + chr8.4:508699-508718 MS.gene43455:CDS 35.0%
!!! CAGGTAGTTTTGAAAGACAT+TGG + chr8.4:508380-508399 MS.gene43455:CDS 35.0%
!!! GGTAGTTTTGAAAGACATTG+GGG + chr8.4:508382-508401 MS.gene43455:CDS 35.0%
!!! GTAGTTTTGAAAGACATTGG+GGG + chr8.4:508383-508402 MS.gene43455:CDS 35.0%
AATCAGCCATAGGAGTAAAC+TGG + chr8.4:506624-506643 MS.gene43455:CDS 40.0%
ACAAGACTATGCGTTCTTTG+AGG + chr8.4:508057-508076 MS.gene43455:CDS 40.0%
AGCTAAAGCCAGAGTTCAAA+AGG - chr8.4:507164-507183 None:intergenic 40.0%
AGGGTTTCATAGATCAGACA+TGG - chr8.4:507280-507299 None:intergenic 40.0%
AGGTTCATGCAATGGAATTG+GGG + chr8.4:508609-508628 MS.gene43455:CDS 40.0%
ATCAGCCATAGGAGTAAACT+GGG + chr8.4:506625-506644 MS.gene43455:CDS 40.0%
ATCCACAAATCAGAAGCAGA+AGG - chr8.4:506830-506849 None:intergenic 40.0%
CAAGACTATGCGTTCTTTGA+GGG + chr8.4:508058-508077 MS.gene43455:CDS 40.0%
CCTACAACAATCCTTAGAGA+AGG + chr8.4:507835-507854 MS.gene43455:CDS 40.0%
CGTTCAGATGTATGGAAACT+AGG - chr8.4:506998-507017 None:intergenic 40.0%
CTTACCACACACCATTTAGA+CGG - chr8.4:508499-508518 None:intergenic 40.0%
GAACTAGCTGAGTCATGATT+TGG - chr8.4:507958-507977 None:intergenic 40.0%
GAATCCTCAATTCCTTCACA+AGG + chr8.4:507911-507930 MS.gene43455:CDS 40.0%
GAGGTTCATGCAATGGAATT+GGG + chr8.4:508608-508627 MS.gene43455:CDS 40.0%
GATAAAGCTACACGTTCAAC+TGG + chr8.4:508775-508794 MS.gene43455:CDS 40.0%
GATTATGGAGGTTCATGCAA+TGG + chr8.4:508601-508620 MS.gene43455:CDS 40.0%
GTTCAGTTCCTCAACTCAAA+CGG + chr8.4:507974-507993 MS.gene43455:CDS 40.0%
TAACGGTCAATTTCAGAACC+TGG + chr8.4:507793-507812 MS.gene43455:CDS 40.0%
TATCCATTAAACCTTGGCCT+TGG + chr8.4:508433-508452 MS.gene43455:CDS 40.0%
TATCCCTTGTGAAGGAATTG+AGG - chr8.4:507918-507937 None:intergenic 40.0%
TCATAGACATCTGCATTGCA+TGG - chr8.4:507893-507912 None:intergenic 40.0%
TCCACAAATCAGAAGCAGAA+GGG - chr8.4:506829-506848 None:intergenic 40.0%
TCTTTGCTCCTTCATCAAGA+AGG - chr8.4:508351-508370 None:intergenic 40.0%
TTTCCAAGGCCAAGGTTTAA+TGG - chr8.4:508439-508458 None:intergenic 40.0%
! ACCATGTTCTTGGTAACAAG+GGG + chr8.4:508278-508297 MS.gene43455:CDS 40.0%
! AGTGAAACTGTTTGAAGCAG+AGG + chr8.4:506718-506737 MS.gene43455:CDS 40.0%
! ATGGCTGATTCTGTCAATAC+AGG - chr8.4:506614-506633 None:intergenic 40.0%
! CAACCATGTTCTTGGTAACA+AGG + chr8.4:508276-508295 MS.gene43455:CDS 40.0%
! CCTTCTCTAAGGATTGTTGT+AGG - chr8.4:507838-507857 None:intergenic 40.0%
! GATGTTCTTAAGGCACTCAT+GGG + chr8.4:506743-506762 MS.gene43455:CDS 40.0%
! GGTCTTATCAACCATGTTCT+TGG + chr8.4:508268-508287 MS.gene43455:CDS 40.0%
! GTATTGACAGAATCAGCCAT+AGG + chr8.4:506614-506633 MS.gene43455:CDS 40.0%
! GTCATGATTTGGGTTAGATC+AGG - chr8.4:507947-507966 None:intergenic 40.0%
! TCAAAGAACGCATAGTCTTG+TGG - chr8.4:508058-508077 None:intergenic 40.0%
! TCAACTGGTGGATCTCAAAT+AGG + chr8.4:508790-508809 MS.gene43455:CDS 40.0%
! TGATGTTCTTAAGGCACTCA+TGG + chr8.4:506742-506761 MS.gene43455:CDS 40.0%
! TGATTTGGGTTAGATCAGGA+CGG - chr8.4:507943-507962 None:intergenic 40.0%
!! ATATCCATAGGAGGAGCATT+AGG - chr8.4:508316-508335 None:intergenic 40.0%
!! CAAAGAACGCATAGTCTTGT+GGG - chr8.4:508057-508076 None:intergenic 40.0%
!! GTGGTAAGTTCTAAGTCAAG+TGG + chr8.4:508511-508530 MS.gene43455:CDS 40.0%
!!! AGCTGCAATTTTGATGGACT+TGG + chr8.4:508694-508713 MS.gene43455:CDS 40.0%
AAAGCTACACGTTCAACTGG+TGG + chr8.4:508778-508797 MS.gene43455:CDS 45.0%
AAGAGATCAACCACAGTCGA+AGG - chr8.4:506677-506696 None:intergenic 45.0%
ACTACTCATCCTGTCACAGA+TGG + chr8.4:508082-508101 MS.gene43455:CDS 45.0%
ACTCATGGGAAGTGGAATTC+AGG + chr8.4:506757-506776 MS.gene43455:CDS 45.0%
AGCAGAGGCTGATGTTCTTA+AGG + chr8.4:506733-506752 MS.gene43455:CDS 45.0%
ATCCTTAGAGAAGGCAAAGC+TGG + chr8.4:507844-507863 MS.gene43455:CDS 45.0%
CAAATTGAAGCCTTCGACTG+TGG + chr8.4:506664-506683 MS.gene43455:CDS 45.0%
CAGTCGAAGGCTTCAATTTG+CGG - chr8.4:506664-506683 None:intergenic 45.0%
CCACAAATCAGAAGCAGAAG+GGG - chr8.4:506828-506847 None:intergenic 45.0%
CTGTTCAGCCTTCTTGATGA+AGG + chr8.4:508340-508359 MS.gene43455:CDS 45.0%
GAAACCCTTAACATGGACAC+CGG + chr8.4:507292-507311 MS.gene43455:intron 45.0%
GGAAATCAGTATCGTCGTAG+AGG - chr8.4:508036-508055 None:intergenic 45.0%
GGAGGTTCATGCAATGGAAT+TGG + chr8.4:508607-508626 MS.gene43455:CDS 45.0%
GTCGCAGCACTCAGTAAAAT+TGG + chr8.4:508151-508170 MS.gene43455:CDS 45.0%
TCAGCCATAGGAGTAAACTG+GGG + chr8.4:506626-506645 MS.gene43455:CDS 45.0%
TCCCCTTGTTACCAAGAACA+TGG - chr8.4:508282-508301 None:intergenic 45.0%
TCTTCCGTCTAAATGGTGTG+TGG + chr8.4:508492-508511 MS.gene43455:CDS 45.0%
TGATGTTGGGTTTATGCTGC+TGG + chr8.4:506585-506604 MS.gene43455:CDS 45.0%
TGCAATGGAATTGGGGACAA+AGG + chr8.4:508616-508635 MS.gene43455:CDS 45.0%
! AGTGGAATTCAGGTGATGGT+TGG + chr8.4:506767-506786 MS.gene43455:CDS 45.0%
! AGTTCAGTTGGTGCAGTACT+TGG - chr8.4:507478-507497 None:intergenic 45.0%
! ATTCCTTGTTTCCAAGGCCA+AGG - chr8.4:508447-508466 None:intergenic 45.0%
! GCATGATAACGAAGTACAGC+AGG - chr8.4:507438-507457 None:intergenic 45.0%
! GTGGAATTCAGGTGATGGTT+GGG + chr8.4:506768-506787 MS.gene43455:CDS 45.0%
! TGTTGTAGGTTGAGTACTGC+AGG - chr8.4:507824-507843 None:intergenic 45.0%
!! AAACCTTGGCCTTGGAAACA+AGG + chr8.4:508441-508460 MS.gene43455:CDS 45.0%
!! GAAATTGGTTGGCCTTCAGA+TGG + chr8.4:508202-508221 MS.gene43455:CDS 45.0%
!! GGAGCAAAGAGTATTCTTCC+AGG + chr8.4:508361-508380 MS.gene43455:CDS 45.0%
ACAGAGCCTTGCAACAGGTT+TGG + chr8.4:506536-506555 MS.gene43455:CDS 50.0%
ACTTCCAGAGACCCTTCAGT+CGG + chr8.4:508724-508743 MS.gene43455:CDS 50.0%
AGAACGTCTCTGCTTACACC+GGG + chr8.4:506855-506874 MS.gene43455:CDS 50.0%
AGCCAGCTTTGCCTTCTCTA+AGG - chr8.4:507849-507868 None:intergenic 50.0%
CAAATCAGAAGCAGAAGGGG+AGG - chr8.4:506825-506844 None:intergenic 50.0%
CAGAACGTCTCTGCTTACAC+CGG + chr8.4:506854-506873 MS.gene43455:CDS 50.0%
CATAGGAGTAAACTGGGGCA+CGG + chr8.4:506631-506650 MS.gene43455:CDS 50.0%
CTTAACATGGACACCGGAGA+CGG + chr8.4:507298-507317 MS.gene43455:intron 50.0%
GATATCAGCACCACCTTTCC+CGG - chr8.4:506876-506895 None:intergenic 50.0%
GCACCAATTGCTCCATCTGA+AGG - chr8.4:508217-508236 None:intergenic 50.0%
GCCATACCAAACCTGTTGCA+AGG - chr8.4:506545-506564 None:intergenic 50.0%
GGACGGAATATCCCTTGTGA+AGG - chr8.4:507926-507945 None:intergenic 50.0%
GGGAAGTGGAATTCAGGTGA+TGG + chr8.4:506763-506782 MS.gene43455:CDS 50.0%
GTAACAAGGGGACACCTTTG+AGG + chr8.4:508290-508309 MS.gene43455:CDS 50.0%
GTCTCCGGTGTCCATGTTAA+GGG - chr8.4:507299-507318 None:intergenic 50.0%
TGGCCTTCAGATGGAGCAAT+TGG + chr8.4:508211-508230 MS.gene43455:CDS 50.0%
! CCCCTTCTGCTTCTGATTTG+TGG + chr8.4:506825-506844 MS.gene43455:CDS 50.0%
! CTTAAGGCACTCATGGGAAG+TGG + chr8.4:506749-506768 MS.gene43455:CDS 50.0%
! GCCTTGCAACAGGTTTGGTA+TGG + chr8.4:506541-506560 MS.gene43455:CDS 50.0%
!! ATGGGGAACCGTTTGAGTTG+AGG - chr8.4:507985-508004 None:intergenic 50.0%
!! GGAAAGGTGGTGCTGATATC+AGG + chr8.4:506876-506895 MS.gene43455:CDS 50.0%
!!! GGCGACTGTCGTTTTATCGT+TGG + chr8.4:508745-508764 MS.gene43455:CDS 50.0%
CAGTATCGTCGTAGAGGCTG+AGG - chr8.4:508030-508049 None:intergenic 55.0%
CGGTGTAAGCAGAGACGTTC+TGG - chr8.4:506856-506875 None:intergenic 55.0%
CGTCGTAGAGGCTGAGGAAA+GGG - chr8.4:508024-508043 None:intergenic 55.0%
CGTCTCCGGTGTCCATGTTA+AGG - chr8.4:507300-507319 None:intergenic 55.0%
CGTGCCCCAGTTTACTCCTA+TGG - chr8.4:506633-506652 None:intergenic 55.0%
GAGGCCTAATGCTCCTCCTA+TGG + chr8.4:508309-508328 MS.gene43455:CDS 55.0%
GTCTCTGCTTACACCGGGAA+AGG + chr8.4:506860-506879 MS.gene43455:CDS 55.0%
TCGTCGTAGAGGCTGAGGAA+AGG - chr8.4:508025-508044 None:intergenic 55.0%
TCTGCTTACACCGGGAAAGG+TGG + chr8.4:506863-506882 MS.gene43455:CDS 55.0%
! AAACGACAGTCGCCGACTGA+AGG - chr8.4:508739-508758 None:intergenic 55.0%
! AACGACAGTCGCCGACTGAA+GGG - chr8.4:508738-508757 None:intergenic 55.0%
! CGATGTGTCATTGCCGTCTC+CGG - chr8.4:507314-507333 None:intergenic 55.0%
! TGAGTACTGCAGGCATGACC+AGG - chr8.4:507814-507833 None:intergenic 55.0%
!! GGAGGAGCATTAGGCCTCAA+AGG - chr8.4:508307-508326 None:intergenic 55.0%
! GTCGCCGACTGAAGGGTCTC+TGG - chr8.4:508731-508750 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr8.4 gene 506533 508882 506533 ID=MS.gene43455
chr8.4 mRNA 506533 508882 506533 ID=MS.gene43455.t1;Parent=MS.gene43455
chr8.4 exon 506533 506897 506533 ID=MS.gene43455.t1.exon1;Parent=MS.gene43455.t1
chr8.4 CDS 506533 506897 506533 ID=cds.MS.gene43455.t1;Parent=MS.gene43455.t1
chr8.4 exon 507754 508882 507754 ID=MS.gene43455.t1.exon2;Parent=MS.gene43455.t1
chr8.4 CDS 507754 508882 507754 ID=cds.MS.gene43455.t1;Parent=MS.gene43455.t1
Gene Sequence

>MS.gene43455

ATGACAGAGCCTTGCAACAGGTTTGGTATGGCTAGTATTTTGACATTAATAATGATGTTGGGTTTATGCTGCTGGATTCCTGTATTGACAGAATCAGCCATAGGAGTAAACTGGGGCACGGTTTCATTCCGCAAATTGAAGCCTTCGACTGTGGTTGATCTCTTAATAGACAACAAAATCACAAAAGTGAAACTGTTTGAAGCAGAGGCTGATGTTCTTAAGGCACTCATGGGAAGTGGAATTCAGGTGATGGTTGGGATTCCCAATGAGATGTTGTTTCTTTTGAGTTCCTCCCCTTCTGCTTCTGATTTGTGGATTCGCCAGAACGTCTCTGCTTACACCGGGAAAGGTGGTGCTGATATCAGGTTAGTAACATGTCATCTAACTTTATTTGTACGTTATCACTGTATATTATTCTTCTATCTTTTTATTCGATCCTCTTTCAAAACCAATAACTATAAGCCTAGTTTCCATACATCTGAACGATTTTGAATAACCTGCAACTGTTCTGAAAGTTTAAATTTTGACATCTTTTACAACAAATAATAAGGATATTTATTGAGTTAGCTTGAAGAACATTGTTTTCATGTTTAAGTAAAATATCAAGTATTCATGTGAAGGAACTTTTCCTTTTGAACTCTGGCTTTAGCTGATCTTCTGATTATTACTGGATAGTATCTCTATAACCAAGAATCTAATTTTTGTTATGTTTAGTGGAAGCTCGAAATTTCATGTCTTTTTGGTCCATGTCTGATCTATGAAACCCTTAACATGGACACCGGAGACGGCAATGACACATCGACATTGATAATAATTTGAGAAACTGGAATAACTGAATACAACTAGGTGTATCATATGCCTTCAATCAAAAGTGTTAGTTCTACAAAGCTTTTGACGTCTATCCTGCTGTACTTCGTTATCATGCTGATGAATAGTGTCATACCAAGTACTGCACCAACTGAACTCTTTTGCATTCTATATGTAATTTGAACTAACAAATTTACTAGGCCATATATATGACTACTTGGGCTTTTAATTGGATAATTAAATATTCTTGTCAATTATTTTAAGTCTTGGTGTGAATAACTCAGATGCCTTTTGCATTTTATTTTTTGGATGTTGTTATTGTGCCTTGATACTTAAATAACTAGAGTTTCTGGAAGTATGTACAAGTTGATTTTCTACAAGTCTTTAAACCTTAGAGGTCTTTTTAAATTACAGGTATGTTGCTGTTGGAAATGAACCATTTCTTAAAAGTTATAACGGTCAATTTCAGAACCTGGTCATGCCTGCAGTACTCAACCTACAACAATCCTTAGAGAAGGCAAAGCTGGCTAGTTCAATAAAACTAGTTGTTCCATGCAATGCAGATGTCTATGAATCCTCAATTCCTTCACAAGGGATATTCCGTCCTGATCTAACCCAAATCATGACTCAGCTAGTTCAGTTCCTCAACTCAAACGGTTCCCCATTTATTGTTAATATCTACCCTTTCCTCAGCCTCTACGACGATACTGATTTCCCACAAGACTATGCGTTCTTTGAGGGAACTACTCATCCTGTCACAGATGGTTCAAATATTTATAGCAATGCATTTGATGGAAACTATGACACTTTAGTCGCAGCACTCAGTAAAATTGGATATGGTCAAATTCCTATAGTTATTGGAGAAATTGGTTGGCCTTCAGATGGAGCAATTGGTGCAAACATCACTGCAGCTAAAGTTTTCAATCAAGGTCTTATCAACCATGTTCTTGGTAACAAGGGGACACCTTTGAGGCCTAATGCTCCTCCTATGGATATCTATCTGTTCAGCCTTCTTGATGAAGGAGCAAAGAGTATTCTTCCAGGTAGTTTTGAAAGACATTGGGGGATATTTTACTTTGATGGAAAAGCTAAGTATCCATTAAACCTTGGCCTTGGAAACAAGGAATTGAAGAAAGCAAAGAATGTTCAGTATCTTCCGTCTAAATGGTGTGTGGTAAGTTCTAAGTCAAGTGGTTTGAGTATTAATAATGTGGCAAACTATATGAGAACTGCATGCAGTGTTGCAGATTGTACAACTCTTGATTATGGAGGTTCATGCAATGGAATTGGGGACAAAGGTAATATATCATATGCTTTTAACAGTTACTATCAACTAAAAATGCAAGACTCAAGAAGCTGCAATTTTGATGGACTTGGTATGGTTACTTCCAGAGACCCTTCAGTCGGCGACTGTCGTTTTATCGTTGGAATTACAGATAAAGCTACACGTTCAACTGGTGGATCTCAAATAGGATATAGTTGGTGGTTTCTATTTCTAGTAGCATTTTCAATTATAGAAATAACTTTTTCACTTTCAATGTGA
Protein sequence

>MS.gene43455.t1

MTEPCNRFGMASILTLIMMLGLCCWIPVLTESAIGVNWGTVSFRKLKPSTVVDLLIDNKITKVKLFEAEADVLKALMGSGIQVMVGIPNEMLFLLSSSPSASDLWIRQNVSAYTGKGGADIRYVAVGNEPFLKSYNGQFQNLVMPAVLNLQQSLEKAKLASSIKLVVPCNADVYESSIPSQGIFRPDLTQIMTQLVQFLNSNGSPFIVNIYPFLSLYDDTDFPQDYAFFEGTTHPVTDGSNIYSNAFDGNYDTLVAALSKIGYGQIPIVIGEIGWPSDGAIGANITAAKVFNQGLINHVLGNKGTPLRPNAPPMDIYLFSLLDEGAKSILPGSFERHWGIFYFDGKAKYPLNLGLGNKELKKAKNVQYLPSKWCVVSSKSSGLSINNVANYMRTACSVADCTTLDYGGSCNGIGDKGNISYAFNSYYQLKMQDSRSCNFDGLGMVTSRDPSVGDCRFIVGITDKATRSTGGSQIGYSWWFLFLVAFSIIEITFSLSM