AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene43528

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene43528.t1 MTR_5g015210 99.335 451 3 0 1 451 1 451 0.0 909
MS.gene43528.t1 MTR_1g017770 62.899 407 145 4 51 451 28 434 4.92e-175 497
MS.gene43528.t1 MTR_8g092870 30.653 398 248 10 63 434 5 400 3.91e-39 146
MS.gene43528.t1 MTR_3g095730 29.383 405 254 8 58 434 1 401 2.96e-35 135
MS.gene43528.t1 MTR_4g085720 28.535 389 264 9 55 430 1 388 4.60e-35 135
MS.gene43528.t1 MTR_5g077510 29.925 401 226 11 67 430 35 417 2.68e-33 130
MS.gene43528.t1 MTR_6g071340 28.784 403 236 10 67 430 5 395 5.51e-33 129
MS.gene43528.t1 MTR_5g048050 29.529 403 233 11 67 430 25 415 3.32e-32 127
MS.gene43528.t1 MTR_7g106340 27.500 400 263 8 66 438 7 406 4.40e-31 124
MS.gene43528.t1 MTR_2g087350 28.389 391 258 9 66 438 5 391 3.99e-29 118
MS.gene43528.t1 MTR_4g063800 27.049 366 245 7 67 417 255 613 8.95e-29 119
MS.gene43528.t1 MTR_3g065080 26.615 387 256 7 67 430 36 417 1.08e-27 114
MS.gene43528.t1 MTR_1g094215 27.193 342 218 6 67 388 64 394 3.16e-27 113
MS.gene43528.t1 MTR_1g094215 27.193 342 218 6 67 388 247 577 1.94e-26 112
MS.gene43528.t1 MTR_7g059405 27.177 379 250 11 67 430 264 631 3.84e-26 112
MS.gene43528.t1 MTR_4g485520 27.195 353 228 11 67 399 105 448 8.00e-26 110
MS.gene43528.t1 MTR_4g485520 27.195 353 228 11 67 399 273 616 1.56e-25 110
MS.gene43528.t1 MTR_7g078330 25.916 382 240 8 67 425 274 635 1.71e-24 107
MS.gene43528.t1 MTR_3g096370 27.577 388 252 11 67 430 10 392 8.25e-24 103
MS.gene43528.t1 MTR_1g056910 26.667 405 233 14 66 429 5 386 3.07e-23 101
MS.gene43528.t1 MTR_1g056870 25.659 417 266 9 66 438 6 422 5.23e-23 101
MS.gene43528.t1 MTR_1g056880 25.659 417 266 9 66 438 6 422 1.95e-22 99.8
MS.gene43528.t1 MTR_1g056840 25.240 416 243 10 66 438 6 396 1.02e-21 97.1
MS.gene43528.t1 MTR_5g083030 25.000 356 227 6 67 389 293 641 6.24e-21 96.3
MS.gene43528.t1 MTR_4g051515 23.040 421 271 12 66 448 5 410 9.20e-21 94.4
MS.gene43528.t1 MTR_4g107010 27.273 363 232 12 52 388 264 620 3.32e-20 94.0
MS.gene43528.t1 MTR_7g116600 26.590 346 213 10 68 388 79 408 1.28e-19 91.3
MS.gene43528.t1 MTR_2g018010 25.887 282 186 5 67 327 292 571 1.26e-18 89.0
MS.gene43528.t1 MTR_7g053260 25.588 340 223 11 68 388 79 407 6.42e-18 86.3
MS.gene43528.t1 MTR_3g078160 26.316 342 226 8 68 388 276 612 1.23e-17 85.9
MS.gene43528.t1 MTR_3g078160 26.316 342 226 8 68 388 276 612 1.31e-17 85.9
MS.gene43528.t1 MTR_5g034440 27.893 337 211 10 38 351 255 582 1.59e-17 85.5
MS.gene43528.t1 MTR_2g011140 25.455 275 188 5 73 337 21 288 1.48e-16 81.6
MS.gene43528.t1 MTR_1g079450 26.766 269 177 8 67 320 63 326 7.72e-16 79.7
MS.gene43528.t1 MTR_3g085610 50.000 72 36 0 68 139 281 352 8.83e-16 80.5
MS.gene43528.t1 MTR_4g028960 25.292 257 158 7 60 287 274 525 4.09e-15 78.2
MS.gene43528.t1 MTR_1g069845 48.485 66 34 0 68 133 125 190 3.35e-14 75.1
MS.gene43528.t1 MTR_1g069845 48.485 66 34 0 68 133 246 311 5.17e-14 74.7
MS.gene43528.t1 MTR_1g069845 48.485 66 34 0 68 133 242 307 5.25e-14 74.7
MS.gene43528.t1 MTR_3g466220 44.578 83 46 0 51 133 217 299 6.13e-14 74.7
MS.gene43528.t1 MTR_3g466220 44.578 83 46 0 51 133 196 278 6.49e-14 74.7
MS.gene43528.t1 MTR_3g078160 26.809 235 156 5 68 286 276 510 6.49e-14 74.3
MS.gene43528.t1 MTR_4g091880 27.190 331 196 10 66 388 4 297 9.81e-14 72.8
MS.gene43528.t1 MTR_4g091880 32.847 137 79 4 66 197 4 132 2.67e-13 71.6
MS.gene43528.t1 MTR_8g077205 46.970 66 35 0 68 133 276 341 4.88e-13 71.6
MS.gene43528.t1 MTR_8g077205 46.970 66 35 0 68 133 164 229 5.25e-13 71.6
MS.gene43528.t1 MTR_1g093965 41.892 74 43 0 80 153 1 74 1.30e-12 67.0
MS.gene43528.t1 MTR_1g093995 41.892 74 43 0 80 153 1 74 1.53e-12 66.6
MS.gene43528.t1 MTR_8g080280 45.714 70 38 0 68 137 283 352 2.84e-12 69.3
MS.gene43528.t1 MTR_8g103227 47.761 67 34 1 68 133 396 462 3.05e-12 69.3
MS.gene43528.t1 MTR_3g115670 29.921 127 81 2 45 163 8 134 3.64e-12 68.9
MS.gene43528.t1 MTR_1g090320 41.237 97 54 2 68 162 514 609 4.09e-12 69.3
MS.gene43528.t1 MTR_1g090320 41.237 97 54 2 68 162 516 611 4.16e-12 68.9
MS.gene43528.t1 MTR_7g097020 44.776 67 37 0 67 133 241 307 7.03e-12 68.2
MS.gene43528.t1 MTR_7g005940 35.398 113 54 3 67 161 595 706 2.58e-11 66.6
MS.gene43528.t1 MTR_7g005940 35.398 113 54 3 67 161 595 706 2.86e-11 66.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene43528.t1 AT3G49810 74.058 451 114 1 1 451 1 448 0.0 669
MS.gene43528.t1 AT5G65920 71.397 451 122 1 1 451 1 444 0.0 649
MS.gene43528.t1 AT5G65920 71.397 451 122 1 1 451 1 444 0.0 649
MS.gene43528.t1 AT3G19380 30.105 382 242 11 57 416 3 381 5.91e-40 149
MS.gene43528.t1 AT1G49780 29.484 407 261 9 66 449 13 416 7.45e-37 140
MS.gene43528.t1 AT2G35930 26.368 402 269 9 63 443 8 403 3.32e-34 132
MS.gene43528.t1 AT5G64660 29.136 405 247 14 63 434 5 402 2.85e-33 130
MS.gene43528.t1 AT3G18710 28.244 393 255 10 67 436 12 400 1.30e-32 128
MS.gene43528.t1 AT3G54850 27.344 384 247 10 67 430 248 619 7.67e-31 125
MS.gene43528.t1 AT5G09800 26.794 418 259 13 60 447 4 404 9.45e-30 120
MS.gene43528.t1 AT1G23030 27.616 344 229 8 67 398 241 576 4.65e-29 120
MS.gene43528.t1 AT1G66160 26.753 385 260 8 60 430 27 403 1.31e-27 114
MS.gene43528.t1 AT1G71020 25.714 350 237 7 67 398 95 439 1.68e-25 108
MS.gene43528.t1 AT3G46510 25.195 385 257 7 67 432 256 628 2.38e-25 109
MS.gene43528.t1 AT3G52450 25.598 418 262 10 58 430 1 414 2.60e-25 108
MS.gene43528.t1 AT1G71020 25.714 350 237 7 67 398 243 587 2.87e-25 109
MS.gene43528.t1 AT5G42340 29.339 242 147 5 67 302 290 513 5.74e-25 108
MS.gene43528.t1 AT2G28830 25.588 340 229 6 68 388 185 519 1.51e-23 103
MS.gene43528.t1 AT5G37490 23.684 380 274 5 67 430 31 410 6.80e-20 92.0
MS.gene43528.t1 AT1G66160 24.675 385 252 9 60 430 27 387 7.32e-19 89.0
MS.gene43528.t1 AT1G29340 30.978 184 108 3 67 232 305 487 4.51e-18 87.4
MS.gene43528.t1 AT3G07360 29.004 231 138 8 55 261 57 285 1.60e-16 82.0
MS.gene43528.t1 AT5G01830 27.143 280 184 6 22 286 230 504 1.73e-16 82.4
MS.gene43528.t1 AT3G11840 53.247 77 34 2 58 133 1 76 8.34e-16 79.7
MS.gene43528.t1 AT4G21350 26.567 335 222 10 66 388 4 326 9.37e-16 79.0
MS.gene43528.t1 AT1G67530 51.429 70 34 0 68 137 273 342 9.88e-15 77.0
MS.gene43528.t1 AT1G67530 51.429 70 34 0 68 137 273 342 9.88e-15 77.0
MS.gene43528.t1 AT1G67530 51.429 70 34 0 68 137 273 342 9.88e-15 77.0
MS.gene43528.t1 AT1G67530 51.429 70 34 0 68 137 273 342 1.05e-14 76.6
MS.gene43528.t1 AT1G24330 50.725 69 34 0 68 136 276 344 4.36e-14 75.1
MS.gene43528.t1 AT1G24330 50.725 69 34 0 68 136 276 344 4.36e-14 75.1
MS.gene43528.t1 AT1G10560 24.679 312 200 8 71 355 292 595 4.91e-14 74.7
MS.gene43528.t1 AT1G27910 50.000 70 35 0 68 137 280 349 7.08e-14 74.3
MS.gene43528.t1 AT1G60190 23.754 341 230 10 73 388 284 619 1.57e-13 73.2
MS.gene43528.t1 AT5G67340 46.269 67 36 0 67 133 240 306 2.48e-12 69.7
MS.gene43528.t1 AT5G67340 46.269 67 36 0 67 133 240 306 2.48e-12 69.7
MS.gene43528.t1 AT3G54790 41.176 68 40 0 67 134 202 269 4.69e-12 68.6
MS.gene43528.t1 AT2G23140 46.032 63 34 0 71 133 237 299 4.88e-12 68.6
MS.gene43528.t1 AT2G23140 46.032 63 34 0 71 133 234 296 5.04e-12 68.6
MS.gene43528.t1 AT2G23140 46.032 63 34 0 71 133 234 296 5.04e-12 68.6
MS.gene43528.t1 AT2G23140 46.032 63 34 0 71 133 45 107 5.10e-12 68.6
MS.gene43528.t1 AT2G23140 46.032 63 34 0 71 133 45 107 5.10e-12 68.6
MS.gene43528.t1 AT3G54790 41.176 68 40 0 67 134 238 305 5.83e-12 68.6
MS.gene43528.t1 AT3G54790 41.176 68 40 0 67 134 238 305 5.83e-12 68.6
MS.gene43528.t1 AT5G65500 43.056 72 41 0 66 137 714 785 3.88e-11 65.9
MS.gene43528.t1 AT5G65500 43.056 72 41 0 66 137 722 793 4.07e-11 65.9

Find 98 sgRNAs with CRISPR-Local

Find 107 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATCTCCAGCCATAGTTTAT+TGG 0.212618 5.3:-8259546 MS.gene43528:CDS
GCAGATTGTTGTTTCAATAT+TGG 0.233260 5.3:+8259107 None:intergenic
GGAATTAGCCCTTTGTGTTT+TGG 0.251271 5.3:-8259354 MS.gene43528:CDS
TGCATTGGTTCAAGAGAAAT+TGG 0.251419 5.3:+8260139 None:intergenic
CTCCAAACACAACACTTTAT+AGG 0.268814 5.3:-8259997 MS.gene43528:CDS
CCATGTATCAGCCTTCTAAA+AGG 0.281315 5.3:-8260354 MS.gene43528:CDS
TGTCCAGTGCAAAGAGTAAC+AGG 0.310547 5.3:+8260112 None:intergenic
TACAACTATGCAAGAGCTTT+GGG 0.339571 5.3:-8260032 MS.gene43528:CDS
CCAACAGCATGTGAAGTAAA+AGG 0.340191 5.3:+8259752 None:intergenic
CAGAACAGTGTGCTTCTATA+AGG 0.348100 5.3:-8259456 MS.gene43528:CDS
ACCTGCAAAGATTTCTTTGA+TGG 0.353869 5.3:-8259663 MS.gene43528:CDS
GAGAATGTTGTTCCTCTCAT+AGG 0.363619 5.3:+8260087 None:intergenic
TCAATCCAACCAATAAACTA+TGG 0.369818 5.3:+8259537 None:intergenic
GCTGTTGGTTCTGAAGTTAT+TGG 0.374377 5.3:-8259737 MS.gene43528:CDS
GGGTATGAGGGTGGTGGTGA+TGG 0.376806 5.3:-8260319 MS.gene43528:CDS
TCGTCGATTGCCGTTGATGC+AGG 0.377688 5.3:-8259173 MS.gene43528:CDS
GTTGATTGTGGTGGTATTGT+AGG 0.378694 5.3:-8260247 MS.gene43528:CDS
TTCAAAGCTACCCTGCCTTC+AGG 0.379046 5.3:+8259314 None:intergenic
AACCTATAAAGTGTTGTGTT+TGG 0.379505 5.3:+8259995 None:intergenic
ACCATCAAAGAAATCTTTGC+AGG 0.386948 5.3:+8259662 None:intergenic
AGCTGTGAAAGATGGGGTTT+TGG 0.389850 5.3:-8260275 MS.gene43528:CDS
CAAAGCTCTTGCATAGTTGT+AGG 0.392894 5.3:+8260034 None:intergenic
AGCTAGAGTTCATGCTTTGA+AGG 0.394200 5.3:-8259864 MS.gene43528:CDS
CAATTAGTTGAATTGTTGTC+TGG 0.396249 5.3:-8259395 MS.gene43528:CDS
TCAGCCTTCTAAAAGGGATA+TGG 0.401063 5.3:-8260347 MS.gene43528:CDS
CTACAACTATGCAAGAGCTT+TGG 0.413732 5.3:-8260033 MS.gene43528:CDS
ATTGTAGGAACTGGTGTTGT+TGG 0.418341 5.3:-8260232 MS.gene43528:CDS
TGAAGGCAGGGTAGCTTTGA+AGG 0.418617 5.3:-8259312 MS.gene43528:CDS
GGATATGGTTGTTGGGTATG+AGG 0.419887 5.3:-8260332 MS.gene43528:CDS
AGTTGAATTGTTGTCTGGTA+TGG 0.429978 5.3:-8259390 MS.gene43528:CDS
CCAGTGTCATTGGATGGTTC+TGG 0.433464 5.3:+8259019 None:intergenic
TCTAAAAGGGATATGGTTGT+TGG 0.433712 5.3:-8260340 MS.gene43528:CDS
GGAGACACTCAAGAAGGTTA+AGG 0.438920 5.3:-8259891 MS.gene43528:CDS
ATCAAAAGTCTCACCATGAT+AGG 0.446610 5.3:+8259272 None:intergenic
AGCGGCAAGCCCTGCATCAA+CGG 0.453178 5.3:+8259163 None:intergenic
TCCAACCAATAAACTATGGC+TGG 0.454386 5.3:+8259541 None:intergenic
TGGTATGGACCATGATTGTT+TGG 0.458739 5.3:-8259375 MS.gene43528:CDS
TGATGGTCAGATTCTAGATC+TGG 0.470143 5.3:-8260302 MS.gene43528:CDS
CTGGACACAGCTGTGAAAGA+TGG 0.472189 5.3:-8260283 MS.gene43528:CDS
CTAAAAGGGATATGGTTGTT+GGG 0.472206 5.3:-8260339 MS.gene43528:CDS
TGGATTGATGAGGCTTGTTA+AGG 0.477377 5.3:-8259522 MS.gene43528:CDS
CATTCTCAAATGGTTTAACA+TGG 0.477905 5.3:-8260071 MS.gene43528:CDS
AAGTGTTGTGTTTGGAGTGA+TGG 0.479659 5.3:+8260003 None:intergenic
CGTCGATTGCCGTTGATGCA+GGG 0.480126 5.3:-8259172 MS.gene43528:CDS
TGGACACAGCTGTGAAAGAT+GGG 0.480846 5.3:-8260282 MS.gene43528:CDS
TCCAGCCATAGTTTATTGGT+TGG 0.483049 5.3:-8259542 MS.gene43528:CDS
TTTATAGGTTAATATATACT+TGG 0.486014 5.3:-8259982 MS.gene43528:CDS
AGAGGAACAACATTCTCAAA+TGG 0.486614 5.3:-8260081 MS.gene43528:CDS
CATGTATCAGCCTTCTAAAA+GGG 0.488247 5.3:-8260353 MS.gene43528:CDS
GCACGTAAGTCTGTGATTGA+TGG 0.491037 5.3:-8259809 MS.gene43528:CDS
TTGGGTGGTGTTGATTGTGG+TGG 0.491523 5.3:-8260256 MS.gene43528:CDS
TGTTAACACCATACCTATCA+TGG 0.494074 5.3:-8259285 MS.gene43528:CDS
CATAGCAATGGAATCTGTCC+TGG 0.495678 5.3:-8259491 MS.gene43528:CDS
GGCAATCGACGAACACTCCT+CGG 0.495695 5.3:+8259184 None:intergenic
AGGAAGAGCTTCTGAGCTTG+TGG 0.496986 5.3:-8259912 MS.gene43528:CDS
ATGGTTGTTGGGTATGAGGG+TGG 0.497015 5.3:-8260328 MS.gene43528:CDS
TGTGGTGGTATTGTAGGAAC+TGG 0.497597 5.3:-8260241 MS.gene43528:CDS
GTTGATTGAAACGTTAATTG+AGG 0.502694 5.3:-8259591 MS.gene43528:CDS
GAGACACTCAAGAAGGTTAA+GGG 0.509193 5.3:-8259890 MS.gene43528:CDS
GATATGGTTGTTGGGTATGA+GGG 0.510561 5.3:-8260331 MS.gene43528:CDS
CTTACACTTGGAAATGAAGA+TGG 0.515200 5.3:+8259046 None:intergenic
GATGGTTGATATCTTGAATG+AGG 0.519499 5.3:-8259645 MS.gene43528:CDS
CTCCTTCTTGTGATTCAAAG+TGG 0.522084 5.3:-8259137 MS.gene43528:CDS
TATCGTCAATACCTGAAGGC+AGG 0.524187 5.3:-8259325 MS.gene43528:CDS
GACCCTGTTACTCTTTGCAC+TGG 0.524861 5.3:-8260115 MS.gene43528:CDS
GAAGGAGCTTCATCAAGTTG+TGG 0.525660 5.3:-8259846 MS.gene43528:CDS
AAGAGGTCTGAAGATGTTCA+AGG 0.532675 5.3:-8259932 MS.gene43528:CDS
GTTGTTGGGTATGAGGGTGG+TGG 0.533466 5.3:-8260325 MS.gene43528:CDS
TTTAATCCAGTGTCATTGGA+TGG 0.544882 5.3:+8259013 None:intergenic
GCAATCGACGAACACTCCTC+GGG 0.548555 5.3:+8259185 None:intergenic
GGGCTAATTCCAAACAATCA+TGG 0.556734 5.3:+8259366 None:intergenic
AGAGTAACAGGGTCTTGCAT+TGG 0.556765 5.3:+8260124 None:intergenic
TAACGAATCCAAAACACAAA+GGG 0.557474 5.3:+8259346 None:intergenic
AAGTCTCACCATGATAGGTA+TGG 0.557498 5.3:+8259277 None:intergenic
GTTTATTGGTTGGATTGATG+AGG 0.566504 5.3:-8259532 MS.gene43528:CDS
GGTTCTGGTAAGCTTACACT+TGG 0.573632 5.3:+8259034 None:intergenic
AGTACTTGTTGATGAAGAAG+AGG 0.579589 5.3:-8259949 MS.gene43528:CDS
TGTTTGCAAGCTCGCACCCG+AGG 0.590908 5.3:-8259201 MS.gene43528:CDS
AATCACAAGAAGGAGCTTAG+CGG 0.593465 5.3:+8259145 None:intergenic
ATGGTTGATATCTTGAATGA+GGG 0.598018 5.3:-8259644 MS.gene43528:CDS
TATAGAAGCACACTGTTCTG+AGG 0.600791 5.3:+8259459 None:intergenic
ATCGTCAATACCTGAAGGCA+GGG 0.601834 5.3:-8259324 MS.gene43528:CDS
AACCACTTTGAATCACAAGA+AGG 0.602714 5.3:+8259135 None:intergenic
GTTCTGAGGAGGCTTAATCC+AGG 0.612490 5.3:+8259473 None:intergenic
ATTCTCAAATGGTTTAACAT+GGG 0.615647 5.3:-8260070 MS.gene43528:CDS
AGAAGCACACTGTTCTGAGG+AGG 0.626107 5.3:+8259462 None:intergenic
ATTGGACAAATGAAAACACT+TGG 0.634832 5.3:+8260157 None:intergenic
TCGTTATCGTCAATACCTGA+AGG 0.636371 5.3:-8259329 MS.gene43528:CDS
ACTATGGCTGGAGATAATCT+CGG 0.640439 5.3:+8259553 None:intergenic
AAGTCTGTGATTGATGGAGG+TGG 0.645793 5.3:-8259803 MS.gene43528:CDS
CGTAAGTCTGTGATTGATGG+AGG 0.663245 5.3:-8259806 MS.gene43528:CDS
GCTTGTGGAGACACTCAAGA+AGG 0.663911 5.3:-8259897 MS.gene43528:CDS
AAGGATAAGAGACATAGCAA+TGG 0.668679 5.3:-8259503 MS.gene43528:CDS
GCACTGGACAAACCTATGAG+AGG 0.669601 5.3:-8260099 MS.gene43528:CDS
ATAACGAATCCAAAACACAA+AGG 0.671691 5.3:+8259345 None:intergenic
GGACACAGCTGTGAAAGATG+GGG 0.688951 5.3:-8260281 MS.gene43528:CDS
AGACTAAAATCAGTTGCACG+CGG 0.721441 5.3:-8259613 MS.gene43528:CDS
GTCCAGTGCAAAGAGTAACA+GGG 0.727176 5.3:+8260113 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTTATAGGTTAATATATACT+TGG - chr5.3:8259401-8259420 MS.gene43528:CDS 15.0%
! ATTCTCAAATGGTTTAACAT+GGG - chr5.3:8259313-8259332 MS.gene43528:CDS 25.0%
!! AAGATTTTGTTAGTTAGCAT+TGG - chr5.3:8259955-8259974 MS.gene43528:CDS 25.0%
ATAACGAATCCAAAACACAA+AGG + chr5.3:8260041-8260060 None:intergenic 30.0%
ATTGGACAAATGAAAACACT+TGG + chr5.3:8259229-8259248 None:intergenic 30.0%
CAATTAGTTGAATTGTTGTC+TGG - chr5.3:8259988-8260007 MS.gene43528:CDS 30.0%
CATTCTCAAATGGTTTAACA+TGG - chr5.3:8259312-8259331 MS.gene43528:CDS 30.0%
GTTGATTGAAACGTTAATTG+AGG - chr5.3:8259792-8259811 MS.gene43528:CDS 30.0%
TAACGAATCCAAAACACAAA+GGG + chr5.3:8260040-8260059 None:intergenic 30.0%
TCAATCCAACCAATAAACTA+TGG + chr5.3:8259849-8259868 None:intergenic 30.0%
! AGTATGCATTGTCAATTTTG+TGG - chr5.3:8260157-8260176 MS.gene43528:CDS 30.0%
! ATGGTTGATATCTTGAATGA+GGG - chr5.3:8259739-8259758 MS.gene43528:CDS 30.0%
! TCTGTTTTGTCTTCTTTACT+TGG - chr5.3:8259607-8259626 MS.gene43528:CDS 30.0%
!! AACCTATAAAGTGTTGTGTT+TGG + chr5.3:8259391-8259410 None:intergenic 30.0%
!! GCAGATTGTTGTTTCAATAT+TGG + chr5.3:8260279-8260298 None:intergenic 30.0%
AACCACTTTGAATCACAAGA+AGG + chr5.3:8260251-8260270 None:intergenic 35.0%
AAGGATAAGAGACATAGCAA+TGG - chr5.3:8259880-8259899 MS.gene43528:CDS 35.0%
ACCATCAAAGAAATCTTTGC+AGG + chr5.3:8259724-8259743 None:intergenic 35.0%
ACCTGCAAAGATTTCTTTGA+TGG - chr5.3:8259720-8259739 MS.gene43528:CDS 35.0%
AGAGGAACAACATTCTCAAA+TGG - chr5.3:8259302-8259321 MS.gene43528:CDS 35.0%
AGTTGAATTGTTGTCTGGTA+TGG - chr5.3:8259993-8260012 MS.gene43528:CDS 35.0%
ATCAAAAGTCTCACCATGAT+AGG + chr5.3:8260114-8260133 None:intergenic 35.0%
CTAAAAGGGATATGGTTGTT+GGG - chr5.3:8259044-8259063 MS.gene43528:CDS 35.0%
CTCCAAACACAACACTTTAT+AGG - chr5.3:8259386-8259405 MS.gene43528:CDS 35.0%
CTTACACTTGGAAATGAAGA+TGG + chr5.3:8260340-8260359 None:intergenic 35.0%
TACAACTATGCAAGAGCTTT+GGG - chr5.3:8259351-8259370 MS.gene43528:CDS 35.0%
TATCTCCAGCCATAGTTTAT+TGG - chr5.3:8259837-8259856 MS.gene43528:CDS 35.0%
TCTAAAAGGGATATGGTTGT+TGG - chr5.3:8259043-8259062 MS.gene43528:CDS 35.0%
TGTTAACACCATACCTATCA+TGG - chr5.3:8260098-8260117 MS.gene43528:CDS 35.0%
! ACAACCATATCCCTTTTAGA+AGG + chr5.3:8259043-8259062 None:intergenic 35.0%
! AGTACTTGTTGATGAAGAAG+AGG - chr5.3:8259434-8259453 MS.gene43528:CDS 35.0%
! CATGTATCAGCCTTCTAAAA+GGG - chr5.3:8259030-8259049 MS.gene43528:CDS 35.0%
! GATGGTTGATATCTTGAATG+AGG - chr5.3:8259738-8259757 MS.gene43528:CDS 35.0%
! GTTTATTGGTTGGATTGATG+AGG - chr5.3:8259851-8259870 MS.gene43528:CDS 35.0%
!! CTTTTAGAAGGCTGATACAT+GGG + chr5.3:8259031-8259050 None:intergenic 35.0%
!! TGCATTGGTTCAAGAGAAAT+TGG + chr5.3:8259247-8259266 None:intergenic 35.0%
AAGAGGTCTGAAGATGTTCA+AGG - chr5.3:8259451-8259470 MS.gene43528:CDS 40.0%
AAGTCTCACCATGATAGGTA+TGG + chr5.3:8260109-8260128 None:intergenic 40.0%
AATCACAAGAAGGAGCTTAG+CGG + chr5.3:8260241-8260260 None:intergenic 40.0%
ACTATGGCTGGAGATAATCT+CGG + chr5.3:8259833-8259852 None:intergenic 40.0%
AGACTAAAATCAGTTGCACG+CGG - chr5.3:8259770-8259789 MS.gene43528:CDS 40.0%
AGCTAGAGTTCATGCTTTGA+AGG - chr5.3:8259519-8259538 MS.gene43528:CDS 40.0%
CAAAGCTCTTGCATAGTTGT+AGG + chr5.3:8259352-8259371 None:intergenic 40.0%
CAGAACAGTGTGCTTCTATA+AGG - chr5.3:8259927-8259946 MS.gene43528:CDS 40.0%
CCAACAGCATGTGAAGTAAA+AGG + chr5.3:8259634-8259653 None:intergenic 40.0%
CCATGTATCAGCCTTCTAAA+AGG - chr5.3:8259029-8259048 MS.gene43528:CDS 40.0%
CTACAACTATGCAAGAGCTT+TGG - chr5.3:8259350-8259369 MS.gene43528:CDS 40.0%
CTCCTTCTTGTGATTCAAAG+TGG - chr5.3:8260246-8260265 MS.gene43528:CDS 40.0%
GAGAATGTTGTTCCTCTCAT+AGG + chr5.3:8259299-8259318 None:intergenic 40.0%
GAGACACTCAAGAAGGTTAA+GGG - chr5.3:8259493-8259512 MS.gene43528:CDS 40.0%
GATATGGTTGTTGGGTATGA+GGG - chr5.3:8259052-8259071 MS.gene43528:CDS 40.0%
GGGCTAATTCCAAACAATCA+TGG + chr5.3:8260020-8260039 None:intergenic 40.0%
GTTGATTGTGGTGGTATTGT+AGG - chr5.3:8259136-8259155 MS.gene43528:CDS 40.0%
TCCAACCAATAAACTATGGC+TGG + chr5.3:8259845-8259864 None:intergenic 40.0%
TCCAGCCATAGTTTATTGGT+TGG - chr5.3:8259841-8259860 MS.gene43528:CDS 40.0%
TCGTTATCGTCAATACCTGA+AGG - chr5.3:8260054-8260073 MS.gene43528:CDS 40.0%
TGATGGTCAGATTCTAGATC+TGG - chr5.3:8259081-8259100 MS.gene43528:CDS 40.0%
TGGTATGGACCATGATTGTT+TGG - chr5.3:8260008-8260027 MS.gene43528:CDS 40.0%
! AAGTGTTGTGTTTGGAGTGA+TGG + chr5.3:8259383-8259402 None:intergenic 40.0%
! CCTTTTACTTCACATGCTGT+TGG - chr5.3:8259631-8259650 MS.gene43528:CDS 40.0%
! GGAATTAGCCCTTTGTGTTT+TGG - chr5.3:8260029-8260048 MS.gene43528:CDS 40.0%
! TATAGAAGCACACTGTTCTG+AGG + chr5.3:8259927-8259946 None:intergenic 40.0%
! TCAGCCTTCTAAAAGGGATA+TGG - chr5.3:8259036-8259055 MS.gene43528:CDS 40.0%
! TCATGGTGAGACTTTTGATG+AGG - chr5.3:8260115-8260134 MS.gene43528:CDS 40.0%
!! ATTGTAGGAACTGGTGTTGT+TGG - chr5.3:8259151-8259170 MS.gene43528:CDS 40.0%
!! CCTTTTAGAAGGCTGATACA+TGG + chr5.3:8259032-8259051 None:intergenic 40.0%
!! GCTGTTGGTTCTGAAGTTAT+TGG - chr5.3:8259646-8259665 MS.gene43528:CDS 40.0%
!! TGGATTGATGAGGCTTGTTA+AGG - chr5.3:8259861-8259880 MS.gene43528:CDS 40.0%
AAGTCTGTGATTGATGGAGG+TGG - chr5.3:8259580-8259599 MS.gene43528:CDS 45.0%
AGAGTAACAGGGTCTTGCAT+TGG + chr5.3:8259262-8259281 None:intergenic 45.0%
ATCGTCAATACCTGAAGGCA+GGG - chr5.3:8260059-8260078 MS.gene43528:CDS 45.0%
CATAGCAATGGAATCTGTCC+TGG - chr5.3:8259892-8259911 MS.gene43528:CDS 45.0%
CGTAAGTCTGTGATTGATGG+AGG - chr5.3:8259577-8259596 MS.gene43528:CDS 45.0%
GCACGTAAGTCTGTGATTGA+TGG - chr5.3:8259574-8259593 MS.gene43528:CDS 45.0%
GGAGACACTCAAGAAGGTTA+AGG - chr5.3:8259492-8259511 MS.gene43528:CDS 45.0%
GGATATGGTTGTTGGGTATG+AGG - chr5.3:8259051-8259070 MS.gene43528:CDS 45.0%
GTCCAGTGCAAAGAGTAACA+GGG + chr5.3:8259273-8259292 None:intergenic 45.0%
TATCGTCAATACCTGAAGGC+AGG - chr5.3:8260058-8260077 MS.gene43528:CDS 45.0%
TGGACACAGCTGTGAAAGAT+GGG - chr5.3:8259101-8259120 MS.gene43528:CDS 45.0%
TGTCCAGTGCAAAGAGTAAC+AGG + chr5.3:8259274-8259293 None:intergenic 45.0%
TGTGGTGGTATTGTAGGAAC+TGG - chr5.3:8259142-8259161 MS.gene43528:CDS 45.0%
! AGCTGTGAAAGATGGGGTTT+TGG - chr5.3:8259108-8259127 MS.gene43528:CDS 45.0%
! GAAGGAGCTTCATCAAGTTG+TGG - chr5.3:8259537-8259556 MS.gene43528:CDS 45.0%
! GCTGTGAAAGATGGGGTTTT+GGG - chr5.3:8259109-8259128 MS.gene43528:CDS 45.0%
! GGTTCTGGTAAGCTTACACT+TGG + chr5.3:8260352-8260371 None:intergenic 45.0%
!!! GTTTTGGGTGGTGTTGATTG+TGG - chr5.3:8259124-8259143 MS.gene43528:CDS 45.0%
AGGAAGAGCTTCTGAGCTTG+TGG - chr5.3:8259471-8259490 MS.gene43528:CDS 50.0%
ATGGTTGTTGGGTATGAGGG+TGG - chr5.3:8259055-8259074 MS.gene43528:CDS 50.0%
CTGGACACAGCTGTGAAAGA+TGG - chr5.3:8259100-8259119 MS.gene43528:CDS 50.0%
GACCCTGTTACTCTTTGCAC+TGG - chr5.3:8259268-8259287 MS.gene43528:CDS 50.0%
GCACTGGACAAACCTATGAG+AGG - chr5.3:8259284-8259303 MS.gene43528:CDS 50.0%
GCTTGTGGAGACACTCAAGA+AGG - chr5.3:8259486-8259505 MS.gene43528:CDS 50.0%
GGACACAGCTGTGAAAGATG+GGG - chr5.3:8259102-8259121 MS.gene43528:CDS 50.0%
TTCAAAGCTACCCTGCCTTC+AGG + chr5.3:8260072-8260091 None:intergenic 50.0%
!! AGAAGCACACTGTTCTGAGG+AGG + chr5.3:8259924-8259943 None:intergenic 50.0%
!! GTTCTGAGGAGGCTTAATCC+AGG + chr5.3:8259913-8259932 None:intergenic 50.0%
!! TGAAGGCAGGGTAGCTTTGA+AGG - chr5.3:8260071-8260090 MS.gene43528:CDS 50.0%
!! TTGGGTGGTGTTGATTGTGG+TGG - chr5.3:8259127-8259146 MS.gene43528:CDS 50.0%
!!! GTGAAAGATGGGGTTTTGGG+TGG - chr5.3:8259112-8259131 MS.gene43528:CDS 50.0%
GCAATCGACGAACACTCCTC+GGG + chr5.3:8260201-8260220 None:intergenic 55.0%
GGCAATCGACGAACACTCCT+CGG + chr5.3:8260202-8260221 None:intergenic 55.0%
GTTGTTGGGTATGAGGGTGG+TGG - chr5.3:8259058-8259077 MS.gene43528:CDS 55.0%
! CGTCGATTGCCGTTGATGCA+GGG - chr5.3:8260211-8260230 MS.gene43528:CDS 55.0%
! TCGTCGATTGCCGTTGATGC+AGG - chr5.3:8260210-8260229 MS.gene43528:CDS 55.0%
AGCGGCAAGCCCTGCATCAA+CGG + chr5.3:8260223-8260242 None:intergenic 60.0%
TGTTTGCAAGCTCGCACCCG+AGG - chr5.3:8260182-8260201 MS.gene43528:CDS 60.0%
! GGGTATGAGGGTGGTGGTGA+TGG - chr5.3:8259064-8259083 MS.gene43528:CDS 60.0%


Chromosome Type Strat End Strand Name
chr5.3 gene 8259025 8260380 8259025 ID=MS.gene43528
chr5.3 mRNA 8259025 8260380 8259025 ID=MS.gene43528.t1;Parent=MS.gene43528
chr5.3 exon 8259025 8260380 8259025 ID=MS.gene43528.t1.exon1;Parent=MS.gene43528.t1
chr5.3 CDS 8259025 8260380 8259025 ID=cds.MS.gene43528.t1;Parent=MS.gene43528.t1
Gene Sequence

>MS.gene43528

ATGCCCATGTATCAGCCTTCTAAAAGGGATATGGTTGTTGGGTATGAGGGTGGTGGTGATGGTCAGATTCTAGATCTGGACACAGCTGTGAAAGATGGGGTTTTGGGTGGTGTTGATTGTGGTGGTATTGTAGGAACTGGTGTTGTTGGTGAGAAATTAGATCTGAGAAAAATGATTCAAGAGCTTGAGTTATGTGAAGTACCAAGTGTTTTCATTTGTCCAATTTCTCTTGAACCAATGCAAGACCCTGTTACTCTTTGCACTGGACAAACCTATGAGAGGAACAACATTCTCAAATGGTTTAACATGGGACACTTTACTTGTCCTACAACTATGCAAGAGCTTTGGGATGATTCCATCACTCCAAACACAACACTTTATAGGTTAATATATACTTGGTTTTCTCAGAAGTACTTGTTGATGAAGAAGAGGTCTGAAGATGTTCAAGGAAGAGCTTCTGAGCTTGTGGAGACACTCAAGAAGGTTAAGGGTCAAGCTAGAGTTCATGCTTTGAAGGAGCTTCATCAAGTTGTGGCTGTTCATGCTACTGCACGTAAGTCTGTGATTGATGGAGGTGGTGTTTCTGTTTTGTCTTCTTTACTTGGTCCTTTTACTTCACATGCTGTTGGTTCTGAAGTTATTGGTATTCTTGTTAGTTTGACACTTGATTCTGAATCTAAAAAGAATCTTATGCAACCTGCAAAGATTTCTTTGATGGTTGATATCTTGAATGAGGGTTCAATTGAGACTAAAATCAGTTGCACGCGGTTGATTGAAACGTTAATTGAGGAGAAAAATTTTCGTTCCGAGATTATCTCCAGCCATAGTTTATTGGTTGGATTGATGAGGCTTGTTAAGGATAAGAGACATAGCAATGGAATCTGTCCTGGATTAAGCCTCCTCAGAACAGTGTGCTTCTATAAGGAAGTTAAGATTTTGTTAGTTAGCATTGGTGCTGTTTCTCAATTAGTTGAATTGTTGTCTGGTATGGACCATGATTGTTTGGAATTAGCCCTTTGTGTTTTGGATTCGTTATCGTCAATACCTGAAGGCAGGGTAGCTTTGAAGGAATGTGTTAACACCATACCTATCATGGTGAGACTTTTGATGAGGATATCAGAAAGCTGTACTCAGTATGCATTGTCAATTTTGTGGTCTGTTTGCAAGCTCGCACCCGAGGAGTGTTCGTCGATTGCCGTTGATGCAGGGCTTGCCGCTAAGCTCCTTCTTGTGATTCAAAGTGGTTGCAATCCAATATTGAAACAACAATCTGCTGAGCTTTTGAAACTATGTAGTTTGAATTATTCAGATACCATCTTCATTTCCAAGTGTAAGCTTACCAGAACCATCCAATGA
Protein sequence

>MS.gene43528.t1

MPMYQPSKRDMVVGYEGGGDGQILDLDTAVKDGVLGGVDCGGIVGTGVVGEKLDLRKMIQELELCEVPSVFICPISLEPMQDPVTLCTGQTYERNNILKWFNMGHFTCPTTMQELWDDSITPNTTLYRLIYTWFSQKYLLMKKRSEDVQGRASELVETLKKVKGQARVHALKELHQVVAVHATARKSVIDGGGVSVLSSLLGPFTSHAVGSEVIGILVSLTLDSESKKNLMQPAKISLMVDILNEGSIETKISCTRLIETLIEEKNFRSEIISSHSLLVGLMRLVKDKRHSNGICPGLSLLRTVCFYKEVKILLVSIGAVSQLVELLSGMDHDCLELALCVLDSLSSIPEGRVALKECVNTIPIMVRLLMRISESCTQYALSILWSVCKLAPEECSSIAVDAGLAAKLLLVIQSGCNPILKQQSAELLKLCSLNYSDTIFISKCKLTRTIQ