Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene43997.t1 | XP_013468974.1 | 95.8 | 144 | 6 | 0 | 1 | 144 | 1 | 144 | 2.30E-75 | 291.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene43997.t1 | P29062 | 71.0 | 138 | 38 | 1 | 8 | 145 | 11 | 146 | 6.0e-56 | 218.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene43997.t1 | A0A072VN05 | 95.8 | 144 | 6 | 0 | 1 | 144 | 1 | 144 | 1.7e-75 | 291.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051980 | MS.gene43997 | 0.81525 | 1.02E-51 | -1.69E-46 |
| MS.gene056981 | MS.gene43997 | 0.826874 | 2.13E-54 | -1.69E-46 |
| MS.gene057841 | MS.gene43997 | 0.807834 | 4.17E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene43997.t1 | MTR_1g080800 | 95.833 | 144 | 6 | 0 | 1 | 144 | 1 | 144 | 9.39e-100 | 283 |
| MS.gene43997.t1 | MTR_1g080820 | 90.278 | 144 | 14 | 0 | 1 | 144 | 1 | 144 | 1.56e-93 | 268 |
| MS.gene43997.t1 | MTR_1g080810 | 84.722 | 144 | 22 | 0 | 1 | 144 | 1 | 144 | 1.67e-89 | 258 |
| MS.gene43997.t1 | MTR_7g115220 | 70.000 | 120 | 36 | 0 | 29 | 148 | 69 | 188 | 2.91e-59 | 183 |
| MS.gene43997.t1 | MTR_1g080780 | 59.124 | 137 | 55 | 1 | 8 | 144 | 4 | 139 | 7.15e-57 | 175 |
| MS.gene43997.t1 | MTR_6g083270 | 52.866 | 157 | 53 | 5 | 10 | 149 | 11 | 163 | 1.39e-46 | 157 |
| MS.gene43997.t1 | MTR_6g083200 | 52.518 | 139 | 59 | 3 | 9 | 145 | 10 | 143 | 3.51e-46 | 150 |
| MS.gene43997.t1 | MTR_6g083290 | 46.479 | 142 | 62 | 3 | 10 | 144 | 11 | 145 | 2.21e-38 | 133 |
| MS.gene43997.t1 | MTR_6g083310 | 49.333 | 150 | 56 | 5 | 10 | 144 | 11 | 155 | 2.56e-38 | 129 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene43997.t1 | AT3G04720 | 65.468 | 139 | 48 | 0 | 24 | 162 | 71 | 209 | 4.40e-62 | 191 |
Find 42 sgRNAs with CRISPR-Local
Find 59 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTGTCATGGCGTAGCAAATA+TGG | 0.308099 | 1.3:-57167242 | MS.gene43997:CDS |
| TCTCTGCCTGTTGGCCCAGC+TGG | 0.347389 | 1.3:+57167200 | None:intergenic |
| ACAGAGAACTTTGAGCTTGT+TGG | 0.365729 | 1.3:-57167414 | MS.gene43997:CDS |
| ACAACCCTCAGAATATTAAC+TGG | 0.383186 | 1.3:-57167316 | MS.gene43997:CDS |
| TGTGGACCAGCTGGGCCAAC+AGG | 0.421055 | 1.3:-57167206 | MS.gene43997:CDS |
| GTCAGAGTGCAAACAACGTT+AGG | 0.437077 | 1.3:-57167355 | MS.gene43997:CDS |
| CCAGTGTATACTGTGCTACT+TGG | 0.451011 | 1.3:-57167280 | MS.gene43997:CDS |
| TAACTGTAAGGTGACCCTTC+TGG | 0.462881 | 1.3:+57166772 | None:intergenic |
| TCAAATTGATACCAATGGAC+AGG | 0.479120 | 1.3:-57166799 | MS.gene43997:CDS |
| CAAAGCTATAGTTAACTGTA+AGG | 0.486461 | 1.3:+57166760 | None:intergenic |
| ATCTAAGTCCAGTCCACCAT+TGG | 0.492671 | 1.3:+57166836 | None:intergenic |
| GAATACTGCAACTGGTGCTC+AGG | 0.493434 | 1.3:-57166886 | MS.gene43997:CDS |
| TGCTACGCCATGACAAGGGC+TGG | 0.498360 | 1.3:+57167249 | None:intergenic |
| CCAGTGCTCCAATGGTGGAC+TGG | 0.503700 | 1.3:-57166844 | MS.gene43997:CDS |
| CCAGTCCACCATTGGAGCAC+TGG | 0.503905 | 1.3:+57166844 | None:intergenic |
| CAGGTGACGAATACTGCAAC+TGG | 0.524837 | 1.3:-57166894 | MS.gene43997:CDS |
| CCGCAAGAATCTCTGCCTGT+TGG | 0.525418 | 1.3:+57167191 | None:intergenic |
| TGGCCCAGCTGGTCCACAAA+AGG | 0.525504 | 1.3:+57167211 | None:intergenic |
| CAGTGTATACTGTGCTACTT+GGG | 0.526139 | 1.3:-57167279 | MS.gene43997:CDS |
| GTGATTTATTGACTCATCAC+TGG | 0.538887 | 1.3:-57166725 | MS.gene43997:CDS |
| TTCAATCAAATTGATACCAA+TGG | 0.541175 | 1.3:-57166804 | MS.gene43997:CDS |
| CTTCTGGTAACCCTGTCCAT+TGG | 0.545362 | 1.3:+57166788 | None:intergenic |
| TAATCCCAGTTAATATTCTG+AGG | 0.556889 | 1.3:+57167311 | None:intergenic |
| AATGGACAGGGTTACCAGAA+GGG | 0.563174 | 1.3:-57166786 | MS.gene43997:CDS |
| ATAGTGGACCAGTGCTCCAA+TGG | 0.570594 | 1.3:-57166852 | MS.gene43997:CDS |
| TGCAACTGGTGCTCAGGTAA+CGG | 0.577038 | 1.3:-57166880 | MS.gene43997:CDS |
| TATAGCTTTGTTAATTGCGA+CGG | 0.577389 | 1.3:-57166747 | MS.gene43997:CDS |
| TCAGAGTGCAAACAACGTTA+GGG | 0.580216 | 1.3:-57167354 | MS.gene43997:CDS |
| CAACCCTCAGAATATTAACT+GGG | 0.582070 | 1.3:-57167315 | MS.gene43997:CDS |
| ATGCAAACCAGCCCTTGTCA+TGG | 0.583448 | 1.3:-57167256 | MS.gene43997:CDS |
| ATATTTGCTACGCCATGACA+AGG | 0.584472 | 1.3:+57167244 | None:intergenic |
| CAAATTGATACCAATGGACA+GGG | 0.591064 | 1.3:-57166798 | MS.gene43997:CDS |
| CATGGCGTAGCAAATATGGT+TGG | 0.604474 | 1.3:-57167238 | MS.gene43997:CDS |
| TCAGGTAACGGTGAGAATAG+TGG | 0.610066 | 1.3:-57166868 | MS.gene43997:CDS |
| CAATGGACAGGGTTACCAGA+AGG | 0.617429 | 1.3:-57166787 | MS.gene43997:CDS |
| GTGGACCAGTGCTCCAATGG+TGG | 0.625188 | 1.3:-57166849 | MS.gene43997:CDS |
| AATCCCAGTTAATATTCTGA+GGG | 0.629321 | 1.3:+57167312 | None:intergenic |
| TCACTAACATCGTTCCCACA+AGG | 0.655781 | 1.3:+57167381 | None:intergenic |
| CCAAGTAGCACAGTATACAC+TGG | 0.657659 | 1.3:+57167280 | None:intergenic |
| TATTTGCTACGCCATGACAA+GGG | 0.664421 | 1.3:+57167245 | None:intergenic |
| CCAACAGGCAGAGATTCTTG+CGG | 0.667208 | 1.3:-57167191 | MS.gene43997:CDS |
| GTGGGAACGATGTTAGTGAG+TGG | 0.748308 | 1.3:-57167377 | MS.gene43997:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTCAATTAATGATAGTATTG+CGG | - | chr1.3:57167182-57167201 | MS.gene43997:CDS | 20.0% |
| ! | TCTAAAAAAATGAGAGAAAG+TGG | + | chr1.3:57166968-57166987 | None:intergenic | 25.0% |
| ! | TTCAATCAAATTGATACCAA+TGG | - | chr1.3:57167310-57167329 | MS.gene43997:CDS | 25.0% |
| !! | TCACTAGATTTCTTCATTTT+GGG | - | chr1.3:57167068-57167087 | MS.gene43997:intron | 25.0% |
| AATCCCAGTTAATATTCTGA+GGG | + | chr1.3:57166805-57166824 | None:intergenic | 30.0% | |
| CAAAGCTATAGTTAACTGTA+AGG | + | chr1.3:57167357-57167376 | None:intergenic | 30.0% | |
| TAATCCCAGTTAATATTCTG+AGG | + | chr1.3:57166806-57166825 | None:intergenic | 30.0% | |
| ! | ATTCTCTTTGACTAGCTAAT+TGG | + | chr1.3:57167143-57167162 | None:intergenic | 30.0% |
| ! | CACTAGATTTCTTCATTTTG+GGG | - | chr1.3:57167069-57167088 | MS.gene43997:intron | 30.0% |
| ! | CTCACTAGATTTCTTCATTT+TGG | - | chr1.3:57167067-57167086 | MS.gene43997:intron | 30.0% |
| !! | TATAGCTTTGTTAATTGCGA+CGG | - | chr1.3:57167367-57167386 | MS.gene43997:CDS | 30.0% |
| ACAACCCTCAGAATATTAAC+TGG | - | chr1.3:57166798-57166817 | MS.gene43997:CDS | 35.0% | |
| CAAATTGATACCAATGGACA+GGG | - | chr1.3:57167316-57167335 | MS.gene43997:CDS | 35.0% | |
| CAACCCTCAGAATATTAACT+GGG | - | chr1.3:57166799-57166818 | MS.gene43997:CDS | 35.0% | |
| GAAATCTAGTGAGTTTGTGT+TGG | + | chr1.3:57167060-57167079 | None:intergenic | 35.0% | |
| TAGCTAGTCAAAGAGAATTG+TGG | - | chr1.3:57167145-57167164 | MS.gene43997:intron | 35.0% | |
| TCAAATTGATACCAATGGAC+AGG | - | chr1.3:57167315-57167334 | MS.gene43997:CDS | 35.0% | |
| !! | GTGATTTATTGACTCATCAC+TGG | - | chr1.3:57167389-57167408 | MS.gene43997:CDS | 35.0% |
| !! | TGTGTTGGTCAGATTTTAGA+GGG | + | chr1.3:57167045-57167064 | None:intergenic | 35.0% |
| !! | TTGTGTTGGTCAGATTTTAG+AGG | + | chr1.3:57167046-57167065 | None:intergenic | 35.0% |
| !!! | TTGGTTTTGTGTTTCCTTGT+GGG | - | chr1.3:57166719-57166738 | MS.gene43997:CDS | 35.0% |
| ACAGAGAACTTTGAGCTTGT+TGG | - | chr1.3:57166700-57166719 | MS.gene43997:CDS | 40.0% | |
| TCAGAGTGCAAACAACGTTA+GGG | - | chr1.3:57166760-57166779 | MS.gene43997:CDS | 40.0% | |
| TTGTCATGGCGTAGCAAATA+TGG | - | chr1.3:57166872-57166891 | MS.gene43997:CDS | 40.0% | |
| ! | ATATTTGCTACGCCATGACA+AGG | + | chr1.3:57166873-57166892 | None:intergenic | 40.0% |
| ! | TATTTGCTACGCCATGACAA+GGG | + | chr1.3:57166872-57166891 | None:intergenic | 40.0% |
| !! | CAGTGTATACTGTGCTACTT+GGG | - | chr1.3:57166835-57166854 | MS.gene43997:CDS | 40.0% |
| !!! | GTTGGTTTTGTGTTTCCTTG+TGG | - | chr1.3:57166718-57166737 | MS.gene43997:CDS | 40.0% |
| AATGGACAGGGTTACCAGAA+GGG | - | chr1.3:57167328-57167347 | MS.gene43997:CDS | 45.0% | |
| ATCTAAGTCCAGTCCACCAT+TGG | + | chr1.3:57167281-57167300 | None:intergenic | 45.0% | |
| CATGGCGTAGCAAATATGGT+TGG | - | chr1.3:57166876-57166895 | MS.gene43997:CDS | 45.0% | |
| CCAAGTAGCACAGTATACAC+TGG | + | chr1.3:57166837-57166856 | None:intergenic | 45.0% | |
| GTATTCGTCACCTGCATCAT+CGG | + | chr1.3:57167214-57167233 | None:intergenic | 45.0% | |
| GTCAGAGTGCAAACAACGTT+AGG | - | chr1.3:57166759-57166778 | MS.gene43997:CDS | 45.0% | |
| TAACTGTAAGGTGACCCTTC+TGG | + | chr1.3:57167345-57167364 | None:intergenic | 45.0% | |
| TCACTAACATCGTTCCCACA+AGG | + | chr1.3:57166736-57166755 | None:intergenic | 45.0% | |
| ! | CCAGTGTATACTGTGCTACT+TGG | - | chr1.3:57166834-57166853 | MS.gene43997:CDS | 45.0% |
| ! | TATGGTTGGACTGCCTTTTG+TGG | - | chr1.3:57166890-57166909 | MS.gene43997:CDS | 45.0% |
| ! | TCAGGTAACGGTGAGAATAG+TGG | - | chr1.3:57167246-57167265 | MS.gene43997:CDS | 45.0% |
| ATGCAAACCAGCCCTTGTCA+TGG | - | chr1.3:57166858-57166877 | MS.gene43997:CDS | 50.0% | |
| CAATGGACAGGGTTACCAGA+AGG | - | chr1.3:57167327-57167346 | MS.gene43997:CDS | 50.0% | |
| CAGGTGACGAATACTGCAAC+TGG | - | chr1.3:57167220-57167239 | MS.gene43997:CDS | 50.0% | |
| CCAACAGGCAGAGATTCTTG+CGG | - | chr1.3:57166923-57166942 | MS.gene43997:intron | 50.0% | |
| CTTCTGGTAACCCTGTCCAT+TGG | + | chr1.3:57167329-57167348 | None:intergenic | 50.0% | |
| GAATACTGCAACTGGTGCTC+AGG | - | chr1.3:57167228-57167247 | MS.gene43997:CDS | 50.0% | |
| GTGGGAACGATGTTAGTGAG+TGG | - | chr1.3:57166737-57166756 | MS.gene43997:CDS | 50.0% | |
| TGCAACTGGTGCTCAGGTAA+CGG | - | chr1.3:57167234-57167253 | MS.gene43997:CDS | 50.0% | |
| !! | ATAGTGGACCAGTGCTCCAA+TGG | - | chr1.3:57167262-57167281 | MS.gene43997:CDS | 50.0% |
| CCGCAAGAATCTCTGCCTGT+TGG | + | chr1.3:57166926-57166945 | None:intergenic | 55.0% | |
| ! | ACTGCCTTTTGTGGACCAGC+TGG | - | chr1.3:57166899-57166918 | MS.gene43997:intron | 55.0% |
| ! | CTGCCTTTTGTGGACCAGCT+GGG | - | chr1.3:57166900-57166919 | MS.gene43997:intron | 55.0% |
| CCAGTCCACCATTGGAGCAC+TGG | + | chr1.3:57167273-57167292 | None:intergenic | 60.0% | |
| GCGGTGATGACCGATGATGC+AGG | - | chr1.3:57167201-57167220 | MS.gene43997:CDS | 60.0% | |
| GTGGACCAGTGCTCCAATGG+TGG | - | chr1.3:57167265-57167284 | MS.gene43997:CDS | 60.0% | |
| TGCTACGCCATGACAAGGGC+TGG | + | chr1.3:57166868-57166887 | None:intergenic | 60.0% | |
| TGGCCCAGCTGGTCCACAAA+AGG | + | chr1.3:57166906-57166925 | None:intergenic | 60.0% | |
| !! | CCAGTGCTCCAATGGTGGAC+TGG | - | chr1.3:57167270-57167289 | MS.gene43997:CDS | 60.0% |
| TCTCTGCCTGTTGGCCCAGC+TGG | + | chr1.3:57166917-57166936 | None:intergenic | 65.0% | |
| TGTGGACCAGCTGGGCCAAC+AGG | - | chr1.3:57166908-57166927 | MS.gene43997:intron | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.3 | gene | 57166689 | 57167447 | 57166689 | ID=MS.gene43997 |
| chr1.3 | mRNA | 57166689 | 57167447 | 57166689 | ID=MS.gene43997.t1;Parent=MS.gene43997 |
| chr1.3 | exon | 57167178 | 57167447 | 57167178 | ID=MS.gene43997.t1.exon1;Parent=MS.gene43997.t1 |
| chr1.3 | CDS | 57167178 | 57167447 | 57167178 | ID=cds.MS.gene43997.t1;Parent=MS.gene43997.t1 |
| chr1.3 | exon | 57166689 | 57166913 | 57166689 | ID=MS.gene43997.t1.exon2;Parent=MS.gene43997.t1 |
| chr1.3 | CDS | 57166689 | 57166913 | 57166689 | ID=cds.MS.gene43997.t1;Parent=MS.gene43997.t1 |
| Gene Sequence |
| Protein sequence |
