Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44132.t1 | XP_003591148.1 | 96.9 | 129 | 4 | 0 | 1 | 129 | 1 | 129 | 8.20E-63 | 249.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44132.t1 | Q5J907 | 87.6 | 129 | 16 | 0 | 1 | 129 | 1 | 129 | 2.3e-58 | 226.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44132.t1 | G7I8P9 | 96.9 | 129 | 4 | 0 | 1 | 129 | 1 | 129 | 5.9e-63 | 249.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene053963 | MS.gene44132 | 0.808578 | 2.89E-50 | -1.69E-46 |
| MS.gene05415 | MS.gene44132 | 0.80102 | 1.10E-48 | -1.69E-46 |
| MS.gene054222 | MS.gene44132 | 0.816911 | 4.33E-52 | -1.69E-46 |
| MS.gene056068 | MS.gene44132 | 0.804731 | 1.88E-49 | -1.69E-46 |
| MS.gene060365 | MS.gene44132 | 0.80406 | 2.59E-49 | -1.69E-46 |
| MS.gene060521 | MS.gene44132 | 0.803652 | 3.15E-49 | -1.69E-46 |
| MS.gene060879 | MS.gene44132 | 0.832428 | 9.51E-56 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44132.t1 | MTR_1g083350 | 96.154 | 130 | 5 | 0 | 1 | 130 | 1 | 130 | 3.11e-88 | 254 |
| MS.gene44132.t1 | MTR_1g083380 | 95.385 | 130 | 6 | 0 | 1 | 130 | 1 | 130 | 3.91e-87 | 251 |
| MS.gene44132.t1 | MTR_1g083340 | 89.286 | 140 | 5 | 1 | 1 | 130 | 1 | 140 | 1.97e-84 | 244 |
| MS.gene44132.t1 | MTR_2g436620 | 80.769 | 130 | 24 | 1 | 1 | 130 | 1 | 129 | 2.17e-71 | 211 |
| MS.gene44132.t1 | MTR_6g071090 | 76.923 | 130 | 29 | 1 | 1 | 130 | 1 | 129 | 2.61e-65 | 196 |
| MS.gene44132.t1 | MTR_2g436620 | 78.846 | 104 | 21 | 1 | 27 | 130 | 1 | 103 | 5.89e-53 | 164 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44132.t1 | AT3G16640 | 76.154 | 130 | 31 | 0 | 1 | 130 | 1 | 130 | 3.21e-68 | 203 |
| MS.gene44132.t1 | AT3G05540 | 76.923 | 130 | 29 | 1 | 1 | 130 | 1 | 129 | 1.07e-62 | 189 |
Find 30 sgRNAs with CRISPR-Local
Find 81 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTGACATTGTTGACACTTTC+AGG | 0.139441 | 1.3:-58930571 | MS.gene44132:CDS |
| ATCCTTGAATAGGAATAATT+TGG | 0.184493 | 1.3:-58930245 | MS.gene44132:intron |
| AATTGTAGGTCTTTGAGTTT+TGG | 0.259902 | 1.3:+58930407 | None:intergenic |
| TGTTGACACTTTCAGGCTTC+AGG | 0.327729 | 1.3:-58930564 | MS.gene44132:CDS |
| GATGAAATACATACAATTGT+AGG | 0.374980 | 1.3:+58930393 | None:intergenic |
| GTTTACCATGATCTTCTTAC+GGG | 0.375564 | 1.3:-58931632 | MS.gene44132:intron |
| TGTTTACCATGATCTTCTTA+CGG | 0.379380 | 1.3:-58931633 | MS.gene44132:CDS |
| CCATTCTGAATTTCCTTGTA+GGG | 0.421805 | 1.3:+58930842 | None:intergenic |
| TCCATTCTGAATTTCCTTGT+AGG | 0.427172 | 1.3:+58930841 | None:intergenic |
| AATTGCTTCTTATCAAAAGC+TGG | 0.428621 | 1.3:+58930536 | None:intergenic |
| GAATGGAATGTTGTGGGAAG+TGG | 0.443613 | 1.3:-58930825 | MS.gene44132:CDS |
| TATTTGCAGTGGGTTGTTCA+AGG | 0.483236 | 1.3:-58930680 | MS.gene44132:intron |
| CTCTGACTCATTCCCCTACA+AGG | 0.486361 | 1.3:-58930855 | MS.gene44132:CDS |
| GGAGCAGTGGATGTGAATAT+TGG | 0.501116 | 1.3:-58930659 | MS.gene44132:CDS |
| GGTGCTAACCCTTCTGCTGA+GGG | 0.509671 | 1.3:-58930638 | MS.gene44132:CDS |
| AATTCAGAATGGAATGTTGT+GGG | 0.534489 | 1.3:-58930831 | MS.gene44132:CDS |
| TCGTCACCACCCTCAGCAGA+AGG | 0.538139 | 1.3:+58930629 | None:intergenic |
| CCCTACAAGGAAATTCAGAA+TGG | 0.541188 | 1.3:-58930842 | MS.gene44132:CDS |
| CATTCTGAATTTCCTTGTAG+GGG | 0.549656 | 1.3:+58930843 | None:intergenic |
| TGGTGCTAACCCTTCTGCTG+AGG | 0.553542 | 1.3:-58930639 | MS.gene44132:CDS |
| GTTTACCCGTAAGAAGATCA+TGG | 0.562866 | 1.3:+58931627 | None:intergenic |
| AAATTCAGAATGGAATGTTG+TGG | 0.574500 | 1.3:-58930832 | MS.gene44132:CDS |
| GTTGTGGGAAGTGGAAGGAA+AGG | 0.585239 | 1.3:-58930816 | MS.gene44132:intron |
| TTCTGCTGAGGGTGGTGACG+AGG | 0.592413 | 1.3:-58930627 | MS.gene44132:CDS |
| AAGATCATGGTAAACAAGCA+TGG | 0.602746 | 1.3:+58931640 | None:intergenic |
| GCTAACCCTTCTGCTGAGGG+TGG | 0.615863 | 1.3:-58930635 | MS.gene44132:CDS |
| CGTCACCACCCTCAGCAGAA+GGG | 0.628440 | 1.3:+58930630 | None:intergenic |
| GTGGGTTGTTCAAGGAGCAG+TGG | 0.636439 | 1.3:-58930672 | MS.gene44132:CDS |
| GAGGGTGGTGACGAGGATGA+CGG | 0.661706 | 1.3:-58930620 | MS.gene44132:CDS |
| GGAATGTTGTGGGAAGTGGA+AGG | 0.687071 | 1.3:-58930821 | MS.gene44132:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTTATTTATGATCATATATT+TGG | + | chr1.3:58931149-58931168 | None:intergenic | 10.0% |
| !!! | TTTAATTTAATAGAATTTGT+TGG | - | chr1.3:58930730-58930749 | MS.gene44132:intron | 10.0% |
| !! | AAAACAAAAATTCATTCAAA+TGG | - | chr1.3:58930947-58930966 | MS.gene44132:intron | 15.0% |
| !! | AGTAATATTTATAGAATAAG+TGG | - | chr1.3:58931565-58931584 | MS.gene44132:intron | 15.0% |
| !!! | TTATATATTAACCTTTTGTA+AGG | - | chr1.3:58930517-58930536 | MS.gene44132:CDS | 15.0% |
| !!! | TTTTTTTTTTTTTTGACACA+AGG | - | chr1.3:58930568-58930587 | MS.gene44132:CDS | 15.0% |
| !! | AAAAGTAAAAGAAACCTAAT+TGG | + | chr1.3:58930312-58930331 | None:intergenic | 20.0% |
| !! | AAAGTAAAAGAAACCTAATT+GGG | + | chr1.3:58930311-58930330 | None:intergenic | 20.0% |
| !! | GATAATAATGAAAAACCTAA+AGG | + | chr1.3:58930704-58930723 | None:intergenic | 20.0% |
| !! | TAATCTTAAATATTAGTAGC+AGG | + | chr1.3:58930897-58930916 | None:intergenic | 20.0% |
| !! | TTATCATTCATCGTAATATT+TGG | + | chr1.3:58930390-58930409 | None:intergenic | 20.0% |
| !!! | ACAAGGTTAAAATACTTTTT+TGG | - | chr1.3:58930585-58930604 | MS.gene44132:CDS | 20.0% |
| !!! | ATTATCTTTCATCTCTTTTT+TGG | - | chr1.3:58931508-58931527 | MS.gene44132:intron | 20.0% |
| !!! | ATTTATAGAATAAGTGGATT+TGG | - | chr1.3:58931571-58931590 | MS.gene44132:intron | 20.0% |
| !!! | TTTTTCATAAACTTCACTTT+TGG | - | chr1.3:58930465-58930484 | MS.gene44132:CDS | 20.0% |
| ! | AAAATCCACAATCAAGTATT+AGG | + | chr1.3:58930857-58930876 | None:intergenic | 25.0% |
| ! | AAATATTACTTAGCAGTTTG+TGG | + | chr1.3:58930808-58930827 | None:intergenic | 25.0% |
| ! | AAATTCATTCACTAAACACA+GGG | + | chr1.3:58930422-58930441 | None:intergenic | 25.0% |
| ! | AATCTTAACAACCTTACAAA+AGG | + | chr1.3:58930531-58930550 | None:intergenic | 25.0% |
| ! | ACTATTATAGATCAAACTTG+TGG | - | chr1.3:58930639-58930658 | MS.gene44132:CDS | 25.0% |
| ! | ATCCAAATTATTCCTATTCA+AGG | + | chr1.3:58931638-58931657 | None:intergenic | 25.0% |
| ! | ATCCTTGAATAGGAATAATT+TGG | - | chr1.3:58931633-58931652 | MS.gene44132:CDS | 25.0% |
| ! | CAAAATCCTATGAAAAACAA+TGG | + | chr1.3:58930337-58930356 | None:intergenic | 25.0% |
| ! | GATGAAATACATACAATTGT+AGG | + | chr1.3:58931488-58931507 | None:intergenic | 25.0% |
| ! | TGGAAAACCTTAAATTTCTT+GGG | - | chr1.3:58930605-58930624 | MS.gene44132:CDS | 25.0% |
| ! | TTGGAAAACCTTAAATTTCT+TGG | - | chr1.3:58930604-58930623 | MS.gene44132:CDS | 25.0% |
| !!! | GAGGGTTTTTTTATTTTGAA+AGG | - | chr1.3:58931530-58931549 | MS.gene44132:intron | 25.0% |
| !!! | TTTTCTCCATTGTTTTTCAT+AGG | - | chr1.3:58930328-58930347 | MS.gene44132:intron | 25.0% |
| AAATTCAGAATGGAATGTTG+TGG | - | chr1.3:58931046-58931065 | MS.gene44132:intron | 30.0% | |
| AATTCAGAATGGAATGTTGT+GGG | - | chr1.3:58931047-58931066 | MS.gene44132:intron | 30.0% | |
| AATTGCTTCTTATCAAAAGC+TGG | + | chr1.3:58931345-58931364 | None:intergenic | 30.0% | |
| ACAAAAAACTCAAAGATGCA+AGG | + | chr1.3:58930276-58930295 | None:intergenic | 30.0% | |
| CACATCACACAATAAACTTT+AGG | + | chr1.3:58931176-58931195 | None:intergenic | 30.0% | |
| GAAATTCATTCACTAAACAC+AGG | + | chr1.3:58930423-58930442 | None:intergenic | 30.0% | |
| GATCTATAATAGTGTGTGTT+TGG | + | chr1.3:58930632-58930651 | None:intergenic | 30.0% | |
| TGTTTACCATGATCTTCTTA+CGG | - | chr1.3:58930245-58930264 | MS.gene44132:intron | 30.0% | |
| ! | CTTTGTTGATGAATTTGTCT+AGG | - | chr1.3:58930988-58931007 | MS.gene44132:intron | 30.0% |
| ! | TAAGTGGATTTGGAAGAATT+TGG | - | chr1.3:58931581-58931600 | MS.gene44132:intron | 30.0% |
| ! | TTTTACTTTACCACCCAATT+AGG | - | chr1.3:58930295-58930314 | MS.gene44132:intron | 30.0% |
| !! | AATTGTAGGTCTTTGAGTTT+TGG | + | chr1.3:58931474-58931493 | None:intergenic | 30.0% |
| !! | AGTTTTATGTTTGTGTTCTG+TGG | - | chr1.3:58930673-58930692 | MS.gene44132:CDS | 30.0% |
| !! | ATTGTAGGTCTTTGAGTTTT+GGG | + | chr1.3:58931473-58931492 | None:intergenic | 30.0% |
| !!! | ATCTTTCATCTCTTTTTTGG+AGG | - | chr1.3:58931511-58931530 | MS.gene44132:intron | 30.0% |
| !!! | TCTTTCATCTCTTTTTTGGA+GGG | - | chr1.3:58931512-58931531 | MS.gene44132:intron | 30.0% |
| CATTCTGAATTTCCTTGTAG+GGG | + | chr1.3:58931038-58931057 | None:intergenic | 35.0% | |
| CCACAATCAAGTATTAGGTT+AGG | + | chr1.3:58930852-58930871 | None:intergenic | 35.0% | |
| CCATTCTGAATTTCCTTGTA+GGG | + | chr1.3:58931039-58931058 | None:intergenic | 35.0% | |
| CCTAACCTAATACTTGATTG+TGG | - | chr1.3:58930849-58930868 | MS.gene44132:CDS | 35.0% | |
| GTAAAAGAAACCTAATTGGG+TGG | + | chr1.3:58930308-58930327 | None:intergenic | 35.0% | |
| GTGATGTGATTATTTGCAGT+GGG | - | chr1.3:58931188-58931207 | MS.gene44132:intron | 35.0% | |
| GTTAGGCTAATCGTGTTTAA+GGG | + | chr1.3:58930835-58930854 | None:intergenic | 35.0% | |
| GTTTACCATGATCTTCTTAC+GGG | - | chr1.3:58930246-58930265 | MS.gene44132:intron | 35.0% | |
| GTTTGGTCCCAAGAAATTTA+AGG | + | chr1.3:58930615-58930634 | None:intergenic | 35.0% | |
| TCAACGTGTTATCCTTGAAT+AGG | - | chr1.3:58931623-58931642 | MS.gene44132:intron | 35.0% | |
| TCCATTCTGAATTTCCTTGT+AGG | + | chr1.3:58931040-58931059 | None:intergenic | 35.0% | |
| TGTGATGTGATTATTTGCAG+TGG | - | chr1.3:58931187-58931206 | MS.gene44132:intron | 35.0% | |
| TGTTCTGTGGTTTAACCTTT+AGG | - | chr1.3:58930686-58930705 | MS.gene44132:intron | 35.0% | |
| TTGACATTGTTGACACTTTC+AGG | - | chr1.3:58931307-58931326 | MS.gene44132:intron | 35.0% | |
| !! | TTGAACAACTCTTGCTTTTC+TGG | + | chr1.3:58931417-58931436 | None:intergenic | 35.0% |
| CCCTACAAGGAAATTCAGAA+TGG | - | chr1.3:58931036-58931055 | MS.gene44132:intron | 40.0% | |
| GGTTAGGCTAATCGTGTTTA+AGG | + | chr1.3:58930836-58930855 | None:intergenic | 40.0% | |
| GTTTACCCGTAAGAAGATCA+TGG | + | chr1.3:58930254-58930273 | None:intergenic | 40.0% | |
| !! | TATTTGCAGTGGGTTGTTCA+AGG | - | chr1.3:58931198-58931217 | MS.gene44132:intron | 40.0% |
| AGTTGCTGTGTTTGTGACAG+AGG | - | chr1.3:58930764-58930783 | MS.gene44132:intron | 45.0% | |
| GAATGGAATGTTGTGGGAAG+TGG | - | chr1.3:58931053-58931072 | MS.gene44132:intron | 45.0% | |
| GGAGCAGTGGATGTGAATAT+TGG | - | chr1.3:58931219-58931238 | MS.gene44132:intron | 45.0% | |
| TGTTGACACTTTCAGGCTTC+AGG | - | chr1.3:58931314-58931333 | MS.gene44132:intron | 45.0% | |
| !!! | GGTCTTTGAGTTTTGGGAGA+AGG | + | chr1.3:58931467-58931486 | None:intergenic | 45.0% |
| !!! | ATTCTATTAAATTAAAATTT+TGG | + | chr1.3:58930726-58930745 | None:intergenic | 5.0% |
| CTCTGACTCATTCCCCTACA+AGG | - | chr1.3:58931023-58931042 | MS.gene44132:intron | 50.0% | |
| GGAATGTTGTGGGAAGTGGA+AGG | - | chr1.3:58931057-58931076 | MS.gene44132:intron | 50.0% | |
| GGGAAGTGGAAGGAAAGGTT+AGG | - | chr1.3:58931067-58931086 | MS.gene44132:intron | 50.0% | |
| GTTGTGGGAAGTGGAAGGAA+AGG | - | chr1.3:58931062-58931081 | MS.gene44132:intron | 50.0% | |
| GGTGCTAACCCTTCTGCTGA+GGG | - | chr1.3:58931240-58931259 | MS.gene44132:intron | 55.0% | |
| ! | GTGGGTTGTTCAAGGAGCAG+TGG | - | chr1.3:58931206-58931225 | MS.gene44132:intron | 55.0% |
| ! | TGGTGCTAACCCTTCTGCTG+AGG | - | chr1.3:58931239-58931258 | MS.gene44132:intron | 55.0% |
| CGTCACCACCCTCAGCAGAA+GGG | + | chr1.3:58931251-58931270 | None:intergenic | 60.0% | |
| GCTAACCCTTCTGCTGAGGG+TGG | - | chr1.3:58931243-58931262 | MS.gene44132:intron | 60.0% | |
| TCGTCACCACCCTCAGCAGA+AGG | + | chr1.3:58931252-58931271 | None:intergenic | 60.0% | |
| ! | GAGGGTGGTGACGAGGATGA+CGG | - | chr1.3:58931258-58931277 | MS.gene44132:intron | 60.0% |
| ! | TTCTGCTGAGGGTGGTGACG+AGG | - | chr1.3:58931251-58931270 | MS.gene44132:intron | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.3 | gene | 58930240 | 58931660 | 58930240 | ID=MS.gene44132 |
| chr1.3 | mRNA | 58930240 | 58931660 | 58930240 | ID=MS.gene44132.t1;Parent=MS.gene44132 |
| chr1.3 | exon | 58931633 | 58931660 | 58931633 | ID=MS.gene44132.t1.exon1;Parent=MS.gene44132.t1 |
| chr1.3 | CDS | 58931633 | 58931660 | 58931633 | ID=cds.MS.gene44132.t1;Parent=MS.gene44132.t1 |
| chr1.3 | exon | 58930817 | 58930890 | 58930817 | ID=MS.gene44132.t1.exon2;Parent=MS.gene44132.t1 |
| chr1.3 | CDS | 58930817 | 58930890 | 58930817 | ID=cds.MS.gene44132.t1;Parent=MS.gene44132.t1 |
| chr1.3 | exon | 58930407 | 58930693 | 58930407 | ID=MS.gene44132.t1.exon3;Parent=MS.gene44132.t1 |
| chr1.3 | CDS | 58930407 | 58930693 | 58930407 | ID=cds.MS.gene44132.t1;Parent=MS.gene44132.t1 |
| chr1.3 | exon | 58930240 | 58930255 | 58930240 | ID=MS.gene44132.t1.exon4;Parent=MS.gene44132.t1 |
| chr1.3 | CDS | 58930240 | 58930255 | 58930240 | ID=cds.MS.gene44132.t1;Parent=MS.gene44132.t1 |
| Gene Sequence |
| Protein sequence |
