AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene056068


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene056068.t1 MTR_1g083350 93.452 168 1 1 1 158 1 168 7.90e-111 312
MS.gene056068.t1 MTR_1g083380 92.857 168 2 1 1 158 1 168 8.27e-110 309
MS.gene056068.t1 MTR_2g436620 80.952 168 21 2 1 158 1 167 5.91e-93 267
MS.gene056068.t1 MTR_1g083340 85.350 157 3 2 1 137 1 157 6.19e-88 254
MS.gene056068.t1 MTR_6g071090 75.776 161 28 2 1 151 1 160 2.21e-80 235
MS.gene056068.t1 MTR_2g436620 78.169 142 20 2 27 158 1 141 1.42e-73 217
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene056068.t1 AT3G16640 73.214 168 35 1 1 158 1 168 4.13e-86 249
MS.gene056068.t1 AT3G05540 76.331 169 28 3 1 158 1 168 1.46e-79 233

Find 36 sgRNAs with CRISPR-Local

Find 88 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATTGTAGGTCTTTGAGTTT+AGG 0.257674 1.4:+67452863 None:intergenic
GTTTACCAGGATCTTCTTAC+AGG 0.263960 1.4:-67453980 MS.gene056068:intron
ATTGTAGGTCTTTGAGTTTA+GGG 0.290821 1.4:+67452864 None:intergenic
CTACTTTGCTTATGGCTTGA+AGG 0.363173 1.4:-67452441 MS.gene056068:CDS
TCCATTCTCAATTTCCTTGT+AGG 0.365251 1.4:+67453296 None:intergenic
GATGAAACACATACAATTGT+AGG 0.393371 1.4:+67452849 None:intergenic
TGCAACCATGCTTGTTTACC+AGG 0.395604 1.4:-67453993 None:intergenic
AATTGAGAATGGAATGCTTT+GGG 0.401755 1.4:-67453286 MS.gene056068:CDS
CCATTCTCAATTTCCTTGTA+GGG 0.404442 1.4:+67453297 None:intergenic
AATTGCTTCTTATCAAAAGC+TGG 0.428621 1.4:+67452992 None:intergenic
GAATGGAATGCTTTGGGAAG+TGG 0.450476 1.4:-67453280 MS.gene056068:CDS
TAAGCAAAGTAGAGAAAAGT+TGG 0.453692 1.4:+67452452 None:intergenic
AAATTGAGAATGGAATGCTT+TGG 0.455334 1.4:-67453287 MS.gene056068:CDS
CCTTGTGTTTGCTTACTACA+AGG 0.455642 1.4:-67452489 MS.gene056068:CDS
CCCTACAAGGAAATTGAGAA+TGG 0.477742 1.4:-67453297 MS.gene056068:CDS
TATTTGCAGTGGGTTGTTCA+AGG 0.483236 1.4:-67453136 MS.gene056068:intron
CTCTGACTCATTCCCCTACA+AGG 0.486361 1.4:-67453310 MS.gene056068:CDS
GGTCTTTGAGTTTAGGGAGA+AGG 0.500494 1.4:+67452870 None:intergenic
GGAGCAGTGGATGTGAATAT+TGG 0.501116 1.4:-67453115 MS.gene056068:CDS
TTATACCTGTAAGAAGATCC+TGG 0.509601 1.4:+67453975 None:intergenic
GGTGCTAATCCTTCTGCTGA+GGG 0.511283 1.4:-67453094 MS.gene056068:CDS
GCTTTGGGAAGTGGAAGGAA+AGG 0.531642 1.4:-67453271 MS.gene056068:intron
GTGTTTGCTTACTACAAGGA+TGG 0.538874 1.4:-67452485 MS.gene056068:CDS
CATTCTCAATTTCCTTGTAG+GGG 0.549656 1.4:+67453298 None:intergenic
TGGTGCTAATCCTTCTGCTG+AGG 0.557106 1.4:-67453095 MS.gene056068:CDS
TCGTCTCCACCCTCAGCAGA+AGG 0.563383 1.4:+67453085 None:intergenic
GGGGAGAGCATGCATGATGA+TGG 0.565967 1.4:-67452515 MS.gene056068:CDS
GCTAATCCTTCTGCTGAGGG+TGG 0.579304 1.4:-67453091 MS.gene056068:CDS
TTCTGCTGAGGGTGGAGACG+AGG 0.580750 1.4:-67453083 MS.gene056068:CDS
AAGATCCTGGTAAACAAGCA+TGG 0.602746 1.4:+67453988 None:intergenic
GTAGAGAAAAGTTGGATCAG+TGG 0.605758 1.4:+67452460 None:intergenic
GAGGGTGGAGACGAGGATGA+TGG 0.616662 1.4:-67453076 MS.gene056068:CDS
CCTTGTAGTAAGCAAACACA+AGG 0.635355 1.4:+67452489 None:intergenic
GTGGGTTGTTCAAGGAGCAG+TGG 0.636439 1.4:-67453128 MS.gene056068:CDS
GGAATGCTTTGGGAAGTGGA+AGG 0.659950 1.4:-67453276 MS.gene056068:CDS
CGATGATCAAGCTGTCAAAG+TGG 0.698235 1.4:-67453050 MS.gene056068:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTTATTTATGAACATATATT+TGG + chr1.4:67453228-67453247 None:intergenic 10.0%
!! TAAACTTAATAAAACCAAAA+AGG + chr1.4:67453602-67453621 None:intergenic 15.0%
!!! AAAATTTTGATAAAACTTGT+TGG - chr1.4:67452801-67452820 MS.gene056068:intron 15.0%
!!! AACAAGTTTTATCAAAATTT+TGG + chr1.4:67452802-67452821 None:intergenic 15.0%
!!! AATTATTTAAAACCAAAATG+AGG + chr1.4:67452609-67452628 None:intergenic 15.0%
!!! ATATTTAAGATTATTGCTTT+TGG - chr1.4:67453007-67453026 MS.gene056068:intron 15.0%
!! AAAAAAATCTCAAAGATGAA+AGG + chr1.4:67452467-67452486 None:intergenic 20.0%
!! AATCAGTTAGGATTATTTAT+TGG + chr1.4:67453849-67453868 None:intergenic 20.0%
!!! AAACTTCTCTGTTTTATATA+TGG + chr1.4:67452638-67452657 None:intergenic 20.0%
!!! AACTTCTCTGTTTTATATAT+GGG + chr1.4:67452637-67452656 None:intergenic 20.0%
!!! TATAAAACAGAGAAGTTTTA+TGG - chr1.4:67452640-67452659 MS.gene056068:intron 20.0%
! AAAATCCACAATCAAGTATT+AGG + chr1.4:67452932-67452951 None:intergenic 25.0%
! AAATATTCATGTAACACTTG+TGG + chr1.4:67452883-67452902 None:intergenic 25.0%
! GAAAAACAAACGAAAACTAA+AGG + chr1.4:67452490-67452509 None:intergenic 25.0%
! GATTAATTACTCAATCAGTT+AGG + chr1.4:67453861-67453880 None:intergenic 25.0%
! TAATGTTGCTAACTTTACTT+TGG - chr1.4:67453051-67453070 MS.gene056068:CDS 25.0%
!! CAAAAAACAGAGAAGTTTTA+AGG - chr1.4:67452689-67452708 MS.gene056068:intron 25.0%
!!! AATCAATGTTACAGTTTTGT+TGG - chr1.4:67453877-67453896 MS.gene056068:intron 25.0%
!!! ATCAATGTTACAGTTTTGTT+GGG - chr1.4:67453878-67453897 MS.gene056068:intron 25.0%
!!! CTTAAAACTTCTCTGTTTTT+TGG + chr1.4:67452691-67452710 None:intergenic 25.0%
!!! GATTGTGGATTTTATTTTCT+TGG - chr1.4:67452939-67452958 MS.gene056068:CDS 25.0%
AAATTGAGAATGGAATGCTT+TGG - chr1.4:67453126-67453145 MS.gene056068:CDS 30.0%
AATTGAGAATGGAATGCTTT+GGG - chr1.4:67453127-67453146 MS.gene056068:CDS 30.0%
AATTGCTTCTTATCAAAAGC+TGG + chr1.4:67453424-67453443 None:intergenic 30.0%
CACAATCAAGTATTAGGTTA+GGG + chr1.4:67452926-67452945 None:intergenic 30.0%
CACATCACACAATAAACTTT+AGG + chr1.4:67453255-67453274 None:intergenic 30.0%
GATGAAACACATACAATTGT+AGG + chr1.4:67453567-67453586 None:intergenic 30.0%
GGTTTAACCTTTAGATTTGA+AGG - chr1.4:67452752-67452771 MS.gene056068:intron 30.0%
GTTAGGGTAATCATGTTTAA+GGG + chr1.4:67452910-67452929 None:intergenic 30.0%
TAAGCAAAGTAGAGAAAAGT+TGG + chr1.4:67453964-67453983 None:intergenic 30.0%
TCCAATTATGCACTAATTAC+AGG + chr1.4:67452563-67452582 None:intergenic 30.0%
TCCTGTAATTAGTGCATAAT+TGG - chr1.4:67452559-67452578 MS.gene056068:intron 30.0%
TTGATATTGTTGACACTTTC+AGG - chr1.4:67453386-67453405 MS.gene056068:intron 30.0%
TTTCACTCCTTCAAATCTAA+AGG + chr1.4:67452762-67452781 None:intergenic 30.0%
! AATTGTAGGTCTTTGAGTTT+AGG + chr1.4:67453553-67453572 None:intergenic 30.0%
! ACTTTTCTCTACTTTGCTTA+TGG - chr1.4:67453964-67453983 MS.gene056068:intron 30.0%
! ATTGTAGGTCTTTGAGTTTA+GGG + chr1.4:67453552-67453571 None:intergenic 30.0%
! GTTTCTCATAAACCTCATTT+TGG - chr1.4:67452594-67452613 MS.gene056068:intron 30.0%
! TCACTATCTTTCATCCTTTT+TGG - chr1.4:67453585-67453604 MS.gene056068:intron 30.0%
!! ATTTTCTTGGTTACTGATTG+TGG - chr1.4:67452952-67452971 MS.gene056068:CDS 30.0%
!!! AAAGTTGTTGTTTTTGTGAC+AGG - chr1.4:67452833-67452852 MS.gene056068:intron 30.0%
!!! AAGTTGTTGTTTTTGTGACA+GGG - chr1.4:67452834-67452853 MS.gene056068:intron 30.0%
!!! AGAATTTTTCAAGACACAGT+TGG + chr1.4:67453795-67453814 None:intergenic 30.0%
!!! TCAATGTTACAGTTTTGTTG+GGG - chr1.4:67453879-67453898 MS.gene056068:intron 30.0%
CATTCTCAATTTCCTTGTAG+GGG + chr1.4:67453118-67453137 None:intergenic 35.0%
CCACAATCAAGTATTAGGTT+AGG + chr1.4:67452927-67452946 None:intergenic 35.0%
CCATTCTCAATTTCCTTGTA+GGG + chr1.4:67453119-67453138 None:intergenic 35.0%
CCTAACCTAATACTTGATTG+TGG - chr1.4:67452924-67452943 MS.gene056068:CDS 35.0%
GGTTAGGGTAATCATGTTTA+AGG + chr1.4:67452911-67452930 None:intergenic 35.0%
GTGATGTGATTATTTGCAGT+GGG - chr1.4:67453267-67453286 MS.gene056068:intron 35.0%
TCCATTCTCAATTTCCTTGT+AGG + chr1.4:67453120-67453139 None:intergenic 35.0%
TGTGATGTGATTATTTGCAG+TGG - chr1.4:67453266-67453285 MS.gene056068:intron 35.0%
TTATACCTGTAAGAAGATCC+TGG + chr1.4:67452441-67452460 None:intergenic 35.0%
! AGTTTTCTGTTTGTGTTCTG+TGG - chr1.4:67452731-67452750 MS.gene056068:intron 35.0%
! CTTTGGCGATGAATTTTTCT+AGG - chr1.4:67453068-67453087 MS.gene056068:CDS 35.0%
!! TTGAACAACTCTTGCTTTTC+TGG + chr1.4:67453496-67453515 None:intergenic 35.0%
!!! AGTTGTTGTTTTTGTGACAG+GGG - chr1.4:67452835-67452854 MS.gene056068:intron 35.0%
CCCTACAAGGAAATTGAGAA+TGG - chr1.4:67453116-67453135 MS.gene056068:CDS 40.0%
CCTTGTAGTAAGCAAACACA+AGG + chr1.4:67453927-67453946 None:intergenic 40.0%
CCTTGTGTTTGCTTACTACA+AGG - chr1.4:67453924-67453943 MS.gene056068:intron 40.0%
CTACTTTGCTTATGGCTTGA+AGG - chr1.4:67453972-67453991 MS.gene056068:intron 40.0%
CTATTTGCCACAATTCAAGG+AGG - chr1.4:67453719-67453738 MS.gene056068:intron 40.0%
GCACTATTTGCCACAATTCA+AGG - chr1.4:67453716-67453735 MS.gene056068:intron 40.0%
GTAGAGAAAAGTTGGATCAG+TGG + chr1.4:67453956-67453975 None:intergenic 40.0%
GTGTTTGCTTACTACAAGGA+TGG - chr1.4:67453928-67453947 MS.gene056068:intron 40.0%
GTTTACCAGGATCTTCTTAC+AGG - chr1.4:67452433-67452452 MS.gene056068:CDS 40.0%
TGTGGCTGAAAAATAGGAAG+TGG - chr1.4:67453742-67453761 MS.gene056068:intron 40.0%
!! TATTTGCAGTGGGTTGTTCA+AGG - chr1.4:67453277-67453296 MS.gene056068:CDS 40.0%
CGATGATCAAGCTGTCAAAG+TGG - chr1.4:67453363-67453382 MS.gene056068:intron 45.0%
GGAGCAGTGGATGTGAATAT+TGG - chr1.4:67453298-67453317 MS.gene056068:CDS 45.0%
TGTTGACACTTTCAGGCTTC+AGG - chr1.4:67453393-67453412 MS.gene056068:intron 45.0%
! AGGAGGTGTGGCTGAAAAAT+AGG - chr1.4:67453736-67453755 MS.gene056068:intron 45.0%
! GGTCTTTGAGTTTAGGGAGA+AGG + chr1.4:67453546-67453565 None:intergenic 45.0%
!! GAATGGAATGCTTTGGGAAG+TGG - chr1.4:67453133-67453152 MS.gene056068:intron 45.0%
AGCCACACCTCCTTGAATTG+TGG + chr1.4:67453729-67453748 None:intergenic 50.0%
CTCTGACTCATTCCCCTACA+AGG - chr1.4:67453103-67453122 MS.gene056068:CDS 50.0%
GGGAAGTGGAAGGAAAGGTT+TGG - chr1.4:67453147-67453166 MS.gene056068:intron 50.0%
GGTGCTAATCCTTCTGCTGA+GGG - chr1.4:67453319-67453338 MS.gene056068:CDS 50.0%
TGCCACAATTCAAGGAGGTG+TGG - chr1.4:67453724-67453743 MS.gene056068:intron 50.0%
! TGGTGCTAATCCTTCTGCTG+AGG - chr1.4:67453318-67453337 MS.gene056068:CDS 50.0%
!! GCTTTGGGAAGTGGAAGGAA+AGG - chr1.4:67453142-67453161 MS.gene056068:intron 50.0%
!! GGAATGCTTTGGGAAGTGGA+AGG - chr1.4:67453137-67453156 MS.gene056068:intron 50.0%
GCTAATCCTTCTGCTGAGGG+TGG - chr1.4:67453322-67453341 MS.gene056068:CDS 55.0%
! GGGGAGAGCATGCATGATGA+TGG - chr1.4:67453898-67453917 MS.gene056068:intron 55.0%
! GTGGGTTGTTCAAGGAGCAG+TGG - chr1.4:67453285-67453304 MS.gene056068:CDS 55.0%
GAGGGTGGAGACGAGGATGA+TGG - chr1.4:67453337-67453356 MS.gene056068:intron 60.0%
TCGTCTCCACCCTCAGCAGA+AGG + chr1.4:67453331-67453350 None:intergenic 60.0%
TTCTGCTGAGGGTGGAGACG+AGG - chr1.4:67453330-67453349 MS.gene056068:intron 60.0%


Chromosome Type Strat End Strand Name
chr1.4 gene 67452427 67454008 67452427 ID=MS.gene056068
chr1.4 mRNA 67452427 67454008 67452427 ID=MS.gene056068.t1;Parent=MS.gene056068
chr1.4 exon 67453981 67454008 67453981 ID=MS.gene056068.t1.exon1;Parent=MS.gene056068.t1
chr1.4 CDS 67453981 67454008 67453981 ID=cds.MS.gene056068.t1;Parent=MS.gene056068.t1
chr1.4 exon 67453272 67453345 67453272 ID=MS.gene056068.t1.exon2;Parent=MS.gene056068.t1
chr1.4 CDS 67453272 67453345 67453272 ID=cds.MS.gene056068.t1;Parent=MS.gene056068.t1
chr1.4 exon 67453051 67453149 67453051 ID=MS.gene056068.t1.exon3;Parent=MS.gene056068.t1
chr1.4 CDS 67453051 67453149 67453051 ID=cds.MS.gene056068.t1;Parent=MS.gene056068.t1
chr1.4 exon 67452863 67453020 67452863 ID=MS.gene056068.t1.exon4;Parent=MS.gene056068.t1
chr1.4 CDS 67452863 67453020 67452863 ID=cds.MS.gene056068.t1;Parent=MS.gene056068.t1
chr1.4 exon 67452427 67452544 67452427 ID=MS.gene056068.t1.exon5;Parent=MS.gene056068.t1
chr1.4 CDS 67452427 67452544 67452427 ID=cds.MS.gene056068.t1;Parent=MS.gene056068.t1
Gene Sequence

>MS.gene056068

ATGCTTGTTTACCAGGATCTTCTTACAGGTATAAAAACCTTTCATCTTTGAGATTTTTTTCCTTTAGTTTTCGTTTGTTTTTCTTTTCATAGAATTTTGATTTTGATGTTGTGTTGAATGTTAAATACTTGTTCCTGTAATTAGTGCATAATTGGATTTTGTGTTATGTTTCTCATAAACCTCATTTTGGTTTTAAATAATTATTGTCCCATATATAAAACAGAGAAGTTTTATGGTTGATCTATAAGTGTTTTTTCTTGTCCAAAAAACAGAGAAGTTTTAAGGTTTTTCAAAAAGAATATGAAGTTTTCTGTTTGTGTTCTGTGGTTTAACCTTTAGATTTGAAGGAGTGAAAGTAACTTTTATTATTTTCCAAAATTTTGATAAAACTTGTTGGTCTAATTTTAAAGTTGTTGTTTTTGTGACAGGGGATTAAATTTATGATTTTAATTACCACAAGTGTTACATGAATATTTTTTACCCTTAAACATGATTACCCTAACCTAATACTTGATTGTGGATTTTATTTTCTTGGTTACTGATTGTGGATTTTAGTTTGTGAGATTGCTAACTGCTACAAATATTTAAGATTATTGCTTTTGGTTGCTGATAGATTGAGTAAAATAATGTTGCTAACTTTACTTTGGCGATGAATTTTTCTAGGTGATGAGCTTCTCTCTGACTCATTCCCCTACAAGGAAATTGAGAATGGAATGCTTTGGGAAGTGGAAGGAAAGGTTTGGAACAAACAAATTTCTTTTTTATAATGTTTCTTTCTATGCTTTATTTTTTATGTCACCAAATATATGTTCATAAATAAATTAGCCTAAAGTTTATTGTGTGATGTGATTATTTGCAGTGGGTTGTTCAAGGAGCAGTGGATGTGAATATTGGTGCTAATCCTTCTGCTGAGGGTGGAGACGAGGATGATGGTGTCGATGATCAAGCTGTCAAAGTGGTTGATATTGTTGACACTTTCAGGCTTCAGGAGCAACCAGCTTTTGATAAGAAGCAATTTGTTACTTATATGAAAAGATATATTAAGTTGCTGACATCTAAATTAGAGCCAGAAAAGCAAGAGTTGTTCAAGAAGCACATTGAAGCAGCAACTAAGTTCCTTCTCCCTAAACTCAAAGACCTACAATTGTATGTGTTTCATCACTATCTTTCATCCTTTTTGGTTTTATTAAGTTTATTCTTTATTGTGTATAAATAAATAAGAGCAATGCTATATCTCTAGAAAAATAACATCAAGAATTCTTCAAGAATAAAGAATAGTCAATCACAATGCACTATTTGCCACAATTCAAGGAGGTGTGGCTGAAAAATAGGAAGTGGACAAATCAATCATGCAATTAAGATTTGCCAACTGTGTCTTGAAAAATTCTTGAAGCTATTTTTCTTGAGAAGTAGCATTTTCCAATAAATAATCCTAACTGATTGAGTAATTAATCAATGTTACAGTTTTGTTGGGGAGAGCATGCATGATGATGGAAGCCTTGTGTTTGCTTACTACAAGGATGGTGCCACTGATCCAACTTTTCTCTACTTTGCTTATGGCTTGAAGGAAATCAAGTGTTAA

Protein sequence

>MS.gene056068.t1

MLVYQDLLTGDELLSDSFPYKEIENGMLWEVEGKWVVQGAVDVNIGANPSAEGGDEDDGVDDQAVKVEQPAFDKKQFVTYMKRYIKLLTSKLEPEKQELFKKHIEAATKFLLPKLKDLQFFVGESMHDDGSLVFAYYKDGATDPTFLYFAYGLKEIKC