Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44450.t1 | XP_024636505.1 | 87.5 | 144 | 17 | 1 | 1 | 143 | 1 | 144 | 1.10E-60 | 242.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44450.t1 | G7JF71 | 87.5 | 144 | 17 | 1 | 1 | 143 | 1 | 144 | 8.1e-61 | 242.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051265 | MS.gene44450 | 0.802568 | 5.28E-49 | -1.69E-46 |
| MS.gene052376 | MS.gene44450 | 0.805876 | 1.08E-49 | -1.69E-46 |
| MS.gene054713 | MS.gene44450 | 0.804786 | 1.83E-49 | -1.69E-46 |
| MS.gene058168 | MS.gene44450 | 0.81127 | 7.61E-51 | -1.69E-46 |
| MS.gene060998 | MS.gene44450 | 0.810855 | 9.37E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44450.t1 | MTR_4g097260 | 87.500 | 144 | 17 | 1 | 1 | 143 | 1 | 144 | 2.93e-87 | 251 |
| MS.gene44450.t1 | MTR_4g097250 | 53.279 | 122 | 43 | 5 | 4 | 121 | 5 | 116 | 1.89e-31 | 109 |
| MS.gene44450.t1 | MTR_3g103290 | 33.553 | 152 | 73 | 7 | 6 | 139 | 7 | 148 | 6.27e-13 | 62.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44450.t1 | AT5G65925 | 33.094 | 139 | 74 | 6 | 1 | 125 | 1 | 134 | 6.68e-12 | 60.1 |
Find 35 sgRNAs with CRISPR-Local
Find 39 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACCTTTACCAGGAACCTTAA+TGG | 0.240275 | 8.1:-25242912 | None:intergenic |
| GCAAAGGAAGCAAGCTCATC+AGG | 0.362279 | 8.1:+25242877 | MS.gene44450:CDS |
| GGTACATGAAGGCACCATTA+AGG | 0.367361 | 8.1:+25242898 | MS.gene44450:CDS |
| CACAAGTGATGATGATTTCA+AGG | 0.407959 | 8.1:+25243067 | MS.gene44450:CDS |
| GAAGGCACCATTAAGGTTCC+TGG | 0.439699 | 8.1:+25242905 | MS.gene44450:CDS |
| ACCATTAAGGTTCCTGGTAA+AGG | 0.453532 | 8.1:+25242911 | MS.gene44450:CDS |
| TCGTAAGGGCTGCGTCCCTT+AGG | 0.469808 | 8.1:+25243093 | MS.gene44450:CDS |
| TTCTACTTCTAGAATAAAGA+AGG | 0.470579 | 8.1:-25243250 | None:intergenic |
| AGATGAAAATCGTGTTGAGT+TGG | 0.472358 | 8.1:+25243121 | MS.gene44450:CDS |
| AGGATTCGATGAAAGTTCTT+CGG | 0.483792 | 8.1:+25243147 | MS.gene44450:CDS |
| GATGAAAATCGTGTTGAGTT+GGG | 0.495351 | 8.1:+25243122 | MS.gene44450:CDS |
| AAGGTTCCTGGTAAAGGTAA+AGG | 0.501075 | 8.1:+25242917 | MS.gene44450:CDS |
| CGGAGTCACAGTGTTGGCAT+TGG | 0.501191 | 8.1:+25243167 | MS.gene44450:CDS |
| AAATCGTGTTGAGTTGGGTA+AGG | 0.512990 | 8.1:+25243127 | MS.gene44450:CDS |
| GTCACAGTGTTGGCATTGGA+AGG | 0.517691 | 8.1:+25243171 | MS.gene44450:CDS |
| GAGTTAACACTGAAGCTCCT+AGG | 0.519744 | 8.1:-25243032 | None:intergenic |
| TCTACTTCTAGAATAAAGAA+GGG | 0.539427 | 8.1:-25243249 | None:intergenic |
| GTAAAGGACATGTACGTCAA+TGG | 0.539882 | 8.1:+25242933 | MS.gene44450:CDS |
| ACATGTCCTTTACCTTTACC+AGG | 0.540520 | 8.1:-25242923 | None:intergenic |
| ATGATTTCAAGGAACTCGTA+AGG | 0.547916 | 8.1:+25243078 | MS.gene44450:CDS |
| TGAAGATGATGATAATATCA+AGG | 0.548852 | 8.1:+25243220 | MS.gene44450:CDS |
| CATGGTTGCATGGTCTACAT+AGG | 0.556865 | 8.1:-25242982 | None:intergenic |
| CTTGAAATCATCATCACTTG+TGG | 0.574189 | 8.1:-25243066 | None:intergenic |
| AGGCAAAATCACGAGAACAT+TGG | 0.577726 | 8.1:-25242962 | None:intergenic |
| GTTCTTCGGAGTCACAGTGT+TGG | 0.605231 | 8.1:+25243161 | MS.gene44450:CDS |
| AACACTGAAGCTCCTAGGAA+AGG | 0.606093 | 8.1:-25243027 | None:intergenic |
| AGGAAAGGAGTAAAATTGCG+TGG | 0.609011 | 8.1:-25243012 | None:intergenic |
| CTATGTAGACCATGCAACCA+TGG | 0.615534 | 8.1:+25242983 | MS.gene44450:CDS |
| AAATTGCGTGGGACATACCA+TGG | 0.616046 | 8.1:-25243000 | None:intergenic |
| CAAGCTCATCAGGTACATGA+AGG | 0.617983 | 8.1:+25242887 | MS.gene44450:CDS |
| CTACTTCTAGAATAAAGAAG+GGG | 0.626161 | 8.1:-25243248 | None:intergenic |
| GGAAAGGAGTAAAATTGCGT+GGG | 0.631505 | 8.1:-25243011 | None:intergenic |
| TGATTTCAAGGAACTCGTAA+GGG | 0.632665 | 8.1:+25243079 | MS.gene44450:CDS |
| TGGGACATACCATGGTTGCA+TGG | 0.637890 | 8.1:-25242992 | None:intergenic |
| TGGAAGGATTGAAGAAGACG+AGG | 0.696092 | 8.1:+25243187 | MS.gene44450:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ACAAAAAAAGATAAGAGCAA+AGG | + | chr8.1:25242861-25242880 | MS.gene44450:CDS | 25.0% |
| ! | TGAAGATGATGATAATATCA+AGG | + | chr8.1:25243220-25243239 | MS.gene44450:CDS | 25.0% |
| !!! | TCTACTTCTAGAATAAAGAA+GGG | - | chr8.1:25243252-25243271 | None:intergenic | 25.0% |
| !!! | TTCTACTTCTAGAATAAAGA+AGG | - | chr8.1:25243253-25243272 | None:intergenic | 25.0% |
| ! | ATTTTCATCTCTGATCCTAA+GGG | - | chr8.1:25243111-25243130 | None:intergenic | 30.0% |
| !! | CTACTTCTAGAATAAAGAAG+GGG | - | chr8.1:25243251-25243270 | None:intergenic | 30.0% |
| AGATGAAAATCGTGTTGAGT+TGG | + | chr8.1:25243121-25243140 | MS.gene44450:CDS | 35.0% | |
| AGGATTCGATGAAAGTTCTT+CGG | + | chr8.1:25243147-25243166 | MS.gene44450:CDS | 35.0% | |
| ATGATTTCAAGGAACTCGTA+AGG | + | chr8.1:25243078-25243097 | MS.gene44450:CDS | 35.0% | |
| CTTGAAATCATCATCACTTG+TGG | - | chr8.1:25243069-25243088 | None:intergenic | 35.0% | |
| GATGAAAATCGTGTTGAGTT+GGG | + | chr8.1:25243122-25243141 | MS.gene44450:CDS | 35.0% | |
| TGATTTCAAGGAACTCGTAA+GGG | + | chr8.1:25243079-25243098 | MS.gene44450:CDS | 35.0% | |
| ! | CACAAGTGATGATGATTTCA+AGG | + | chr8.1:25243067-25243086 | MS.gene44450:CDS | 35.0% |
| ! | GATTTTCATCTCTGATCCTA+AGG | - | chr8.1:25243112-25243131 | None:intergenic | 35.0% |
| AAGGTTCCTGGTAAAGGTAA+AGG | + | chr8.1:25242917-25242936 | MS.gene44450:CDS | 40.0% | |
| ACATGTCCTTTACCTTTACC+AGG | - | chr8.1:25242926-25242945 | None:intergenic | 40.0% | |
| ACCATTAAGGTTCCTGGTAA+AGG | + | chr8.1:25242911-25242930 | MS.gene44450:CDS | 40.0% | |
| ACCTTTACCAGGAACCTTAA+TGG | - | chr8.1:25242915-25242934 | None:intergenic | 40.0% | |
| AGGAAAGGAGTAAAATTGCG+TGG | - | chr8.1:25243015-25243034 | None:intergenic | 40.0% | |
| AGGCAAAATCACGAGAACAT+TGG | - | chr8.1:25242965-25242984 | None:intergenic | 40.0% | |
| GGAAAGGAGTAAAATTGCGT+GGG | - | chr8.1:25243014-25243033 | None:intergenic | 40.0% | |
| GTAAAGGACATGTACGTCAA+TGG | + | chr8.1:25242933-25242952 | MS.gene44450:CDS | 40.0% | |
| ! | CGCAATTTTACTCCTTTCCT+AGG | + | chr8.1:25243015-25243034 | MS.gene44450:CDS | 40.0% |
| !! | AAATCGTGTTGAGTTGGGTA+AGG | + | chr8.1:25243127-25243146 | MS.gene44450:CDS | 40.0% |
| AAATTGCGTGGGACATACCA+TGG | - | chr8.1:25243003-25243022 | None:intergenic | 45.0% | |
| AACACTGAAGCTCCTAGGAA+AGG | - | chr8.1:25243030-25243049 | None:intergenic | 45.0% | |
| CAAGCTCATCAGGTACATGA+AGG | + | chr8.1:25242887-25242906 | MS.gene44450:CDS | 45.0% | |
| CATGGTTGCATGGTCTACAT+AGG | - | chr8.1:25242985-25243004 | None:intergenic | 45.0% | |
| CTATGTAGACCATGCAACCA+TGG | + | chr8.1:25242983-25243002 | MS.gene44450:CDS | 45.0% | |
| GAGTTAACACTGAAGCTCCT+AGG | - | chr8.1:25243035-25243054 | None:intergenic | 45.0% | |
| TGGAAGGATTGAAGAAGACG+AGG | + | chr8.1:25243187-25243206 | MS.gene44450:CDS | 45.0% | |
| !! | GGTACATGAAGGCACCATTA+AGG | + | chr8.1:25242898-25242917 | MS.gene44450:CDS | 45.0% |
| GCAAAGGAAGCAAGCTCATC+AGG | + | chr8.1:25242877-25242896 | MS.gene44450:CDS | 50.0% | |
| GTTCTTCGGAGTCACAGTGT+TGG | + | chr8.1:25243161-25243180 | MS.gene44450:CDS | 50.0% | |
| TGGGACATACCATGGTTGCA+TGG | - | chr8.1:25242995-25243014 | None:intergenic | 50.0% | |
| !! | GAAGGCACCATTAAGGTTCC+TGG | + | chr8.1:25242905-25242924 | MS.gene44450:CDS | 50.0% |
| !! | GTCACAGTGTTGGCATTGGA+AGG | + | chr8.1:25243171-25243190 | MS.gene44450:CDS | 50.0% |
| !! | CGGAGTCACAGTGTTGGCAT+TGG | + | chr8.1:25243167-25243186 | MS.gene44450:CDS | 55.0% |
| TCGTAAGGGCTGCGTCCCTT+AGG | + | chr8.1:25243093-25243112 | MS.gene44450:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.1 | gene | 25242858 | 25243313 | 25242858 | ID=MS.gene44450 |
| chr8.1 | mRNA | 25242858 | 25243313 | 25242858 | ID=MS.gene44450.t1;Parent=MS.gene44450 |
| chr8.1 | exon | 25242858 | 25243313 | 25242858 | ID=MS.gene44450.t1.exon1;Parent=MS.gene44450.t1 |
| chr8.1 | CDS | 25242858 | 25243313 | 25242858 | ID=cds.MS.gene44450.t1;Parent=MS.gene44450.t1 |
| Gene Sequence |
| Protein sequence |
