AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene054713


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene054713.t1 MTR_5g045300 97.701 261 6 0 1 261 1 261 0.0 519
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 47 sgRNAs with CRISPR-Local

Find 56 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTATTTCCTTATTCTTTAA+TGG 0.128917 5.3:-32843871 MS.gene054713:CDS
CTTGAGTGTCATGCTCATTT+TGG 0.177443 5.3:-32844222 MS.gene054713:CDS
GGTTTCTTCAACCTTGTTAT+TGG 0.181137 5.3:-32844087 MS.gene054713:CDS
AGTACCCTCTTTAGTTTATT+GGG 0.199150 5.3:-32844181 MS.gene054713:CDS
TACATTTCCATTAAAGAATA+AGG 0.220724 5.3:+32843864 None:intergenic
AGCTATATCTTGGGTTAGTT+TGG 0.236897 5.3:-32843788 MS.gene054713:CDS
TTCTATGTTTGTGTGGATTA+AGG 0.264606 5.3:-32843761 MS.gene054713:CDS
GAGTACCCTCTTTAGTTTAT+TGG 0.311311 5.3:-32844182 MS.gene054713:CDS
TTTGGAATCTTTGTTGGGAT+TGG 0.327665 5.3:-32844204 MS.gene054713:CDS
GTTTCCCAATACCAATCTAA+TGG 0.354508 5.3:+32844144 None:intergenic
GCAGCCCATTAGATTGGTAT+TGG 0.375445 5.3:-32844149 MS.gene054713:CDS
AAGTAAAGGAGGTTCGTAAA+AGG 0.385552 5.3:+32843939 None:intergenic
ATGTTGGTAAAGCTATATCT+TGG 0.394828 5.3:-32843798 MS.gene054713:CDS
GCTATATCTTGGGTTAGTTT+GGG 0.395217 5.3:-32843787 MS.gene054713:CDS
CAGCCCATTAGATTGGTATT+GGG 0.406163 5.3:-32844148 MS.gene054713:CDS
AATTTGAAGTATAAACTTAG+TGG 0.421521 5.3:+32844249 None:intergenic
TGTAACTAGTTCTTGATCCT+TGG 0.433441 5.3:+32843639 None:intergenic
TAAGATTAAACTCAAAGGGT+TGG 0.466105 5.3:-32844004 MS.gene054713:CDS
TGCTCAATCGTTTAATTGAG+TGG 0.467954 5.3:-32844038 MS.gene054713:CDS
ATCCTTGCTTTCATGAATGA+AGG 0.475263 5.3:-32844330 MS.gene054713:CDS
TTTACGAACCTCCTTTACTT+AGG 0.475789 5.3:-32843936 MS.gene054713:CDS
TTTCCCAATACCAATCTAAT+GGG 0.484056 5.3:+32844145 None:intergenic
ATCGTAAGATTAAACTCAAA+GGG 0.489443 5.3:-32844008 MS.gene054713:CDS
ACTATGTCTTGAGAAGAAAC+TGG 0.491172 5.3:+32843913 None:intergenic
CAACTCGCAGCCCATTAGAT+TGG 0.497410 5.3:-32844155 MS.gene054713:CDS
TGTTGGTAAAGCTATATCTT+GGG 0.500531 5.3:-32843797 MS.gene054713:CDS
CGTCAATGAACAATGGTCAT+GGG 0.519308 5.3:-32844283 MS.gene054713:CDS
TTGTGTGCCTTCTCTTCAAG+TGG 0.521153 5.3:+32843706 None:intergenic
GAAGAAACTGGCCTAAGTAA+AGG 0.522889 5.3:+32843925 None:intergenic
TCGTCAATGAACAATGGTCA+TGG 0.527571 5.3:-32844284 MS.gene054713:CDS
GTACCCTCTTTAGTTTATTG+GGG 0.529611 5.3:-32844180 MS.gene054713:CDS
TTCTCTTCAAGTGGTATCTG+TGG 0.535124 5.3:+32843715 None:intergenic
TGCACCCCAATAAACTAAAG+AGG 0.538582 5.3:+32844176 None:intergenic
CAGATTTACACACATGATGT+TGG 0.543563 5.3:-32843814 MS.gene054713:CDS
GATCGTAAGATTAAACTCAA+AGG 0.575574 5.3:-32844009 MS.gene054713:CDS
ACAGATACCACTTGAAGAGA+AGG 0.584939 5.3:-32843713 MS.gene054713:CDS
TGGGAAGTTCTATGTTTGTG+TGG 0.587350 5.3:-32843768 MS.gene054713:CDS
AGATGACAAGAAAGAGTCCA+AGG 0.595725 5.3:-32843656 MS.gene054713:CDS
GACGAAGCTATTAGAGAGTG+AGG 0.597104 5.3:+32844302 None:intergenic
AGCCTTCATTCATGAAAGCA+AGG 0.601961 5.3:+32844328 None:intergenic
GAAACTGGCCTAAGTAAAGG+AGG 0.623329 5.3:+32843928 None:intergenic
GCACCCCAATAAACTAAAGA+GGG 0.630654 5.3:+32844177 None:intergenic
AAGCAAGGATACATATTGAG+AGG 0.635812 5.3:+32844343 None:intergenic
GTCAATGAACAATGGTCATG+GGG 0.649937 5.3:-32844282 MS.gene054713:CDS
GAAGCTATTAGAGAGTGAGG+AGG 0.662308 5.3:+32844305 None:intergenic
ATAGCTTCGTCAATGAACAA+TGG 0.663464 5.3:-32844290 MS.gene054713:CDS
AATGTAGCAATCCAATAACA+AGG 0.681282 5.3:+32844076 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CTTATTTCCTTATTCTTTAA+TGG - chr5.3:32844134-32844153 MS.gene054713:CDS 20.0%
!! TACATTTCCATTAAAGAATA+AGG + chr5.3:32844144-32844163 None:intergenic 20.0%
!!! AATGTTGAAATTTTGAAAAC+TGG - chr5.3:32843897-32843916 MS.gene054713:CDS 20.0%
!!! AATTTGAAGTATAAACTTAG+TGG + chr5.3:32843759-32843778 None:intergenic 20.0%
! ATCGTAAGATTAAACTCAAA+GGG - chr5.3:32843997-32844016 MS.gene054713:CDS 25.0%
!! TGAATGAACTTTTCAATATC+AGG + chr5.3:32843639-32843658 None:intergenic 25.0%
!!! GAAATGTATTGCTTTTAAGT+TGG - chr5.3:32844156-32844175 MS.gene054713:CDS 25.0%
AATGTAGCAATCCAATAACA+AGG + chr5.3:32843932-32843951 None:intergenic 30.0%
GATCGTAAGATTAAACTCAA+AGG - chr5.3:32843996-32844015 MS.gene054713:CDS 30.0%
TAAGATTAAACTCAAAGGGT+TGG - chr5.3:32844001-32844020 MS.gene054713:CDS 30.0%
TTTCCCAATACCAATCTAAT+GGG + chr5.3:32843863-32843882 None:intergenic 30.0%
! AGTACCCTCTTTAGTTTATT+GGG - chr5.3:32843824-32843843 MS.gene054713:CDS 30.0%
! ATGTTGGTAAAGCTATATCT+TGG - chr5.3:32844207-32844226 MS.gene054713:CDS 30.0%
! CCAACTCTGAATTTTTCTTA+GGG + chr5.3:32844037-32844056 None:intergenic 30.0%
! TCCAACTCTGAATTTTTCTT+AGG + chr5.3:32844038-32844057 None:intergenic 30.0%
! TGTTGGTAAAGCTATATCTT+GGG - chr5.3:32844208-32844227 MS.gene054713:CDS 30.0%
! TTCTATGTTTGTGTGGATTA+AGG - chr5.3:32844244-32844263 MS.gene054713:CDS 30.0%
!!! CTCATTTTGGAATCTTTGTT+GGG - chr5.3:32843796-32843815 MS.gene054713:CDS 30.0%
!!! CTTTTAAGTTGGTGTTTTTC+TGG - chr5.3:32844167-32844186 MS.gene054713:CDS 30.0%
AAGCAAGGATACATATTGAG+AGG + chr5.3:32843665-32843684 None:intergenic 35.0%
AAGTAAAGGAGGTTCGTAAA+AGG + chr5.3:32844069-32844088 None:intergenic 35.0%
ACTATGTCTTGAGAAGAAAC+TGG + chr5.3:32844095-32844114 None:intergenic 35.0%
ATAGCTTCGTCAATGAACAA+TGG - chr5.3:32843715-32843734 MS.gene054713:CDS 35.0%
ATCCTTGCTTTCATGAATGA+AGG - chr5.3:32843675-32843694 MS.gene054713:CDS 35.0%
CAGATTTACACACATGATGT+TGG - chr5.3:32844191-32844210 MS.gene054713:CDS 35.0%
CCCTAAGAAAAATTCAGAGT+TGG - chr5.3:32844034-32844053 MS.gene054713:CDS 35.0%
GTTTCCCAATACCAATCTAA+TGG + chr5.3:32843864-32843883 None:intergenic 35.0%
TGCTCAATCGTTTAATTGAG+TGG - chr5.3:32843967-32843986 MS.gene054713:CDS 35.0%
TTTACGAACCTCCTTTACTT+AGG - chr5.3:32844069-32844088 MS.gene054713:CDS 35.0%
! GAGTACCCTCTTTAGTTTAT+TGG - chr5.3:32843823-32843842 MS.gene054713:CDS 35.0%
! GGTTTCTTCAACCTTGTTAT+TGG - chr5.3:32843918-32843937 MS.gene054713:CDS 35.0%
! GTACCCTCTTTAGTTTATTG+GGG - chr5.3:32843825-32843844 MS.gene054713:CDS 35.0%
! TGTAACTAGTTCTTGATCCT+TGG + chr5.3:32844369-32844388 None:intergenic 35.0%
! TTTGGAATCTTTGTTGGGAT+TGG - chr5.3:32843801-32843820 MS.gene054713:CDS 35.0%
!! AGCTATATCTTGGGTTAGTT+TGG - chr5.3:32844217-32844236 MS.gene054713:CDS 35.0%
!! GCTATATCTTGGGTTAGTTT+GGG - chr5.3:32844218-32844237 MS.gene054713:CDS 35.0%
!!! GCTCATTTTGGAATCTTTGT+TGG - chr5.3:32843795-32843814 MS.gene054713:CDS 35.0%
ACAGATACCACTTGAAGAGA+AGG - chr5.3:32844292-32844311 MS.gene054713:CDS 40.0%
AGATGACAAGAAAGAGTCCA+AGG - chr5.3:32844349-32844368 MS.gene054713:CDS 40.0%
AGCCTTCATTCATGAAAGCA+AGG + chr5.3:32843680-32843699 None:intergenic 40.0%
CAGCCCATTAGATTGGTATT+GGG - chr5.3:32843857-32843876 MS.gene054713:CDS 40.0%
CGTCAATGAACAATGGTCAT+GGG - chr5.3:32843722-32843741 MS.gene054713:CDS 40.0%
GCACCCCAATAAACTAAAGA+GGG + chr5.3:32843831-32843850 None:intergenic 40.0%
GTCAATGAACAATGGTCATG+GGG - chr5.3:32843723-32843742 MS.gene054713:CDS 40.0%
TCGTCAATGAACAATGGTCA+TGG - chr5.3:32843721-32843740 MS.gene054713:CDS 40.0%
TGCACCCCAATAAACTAAAG+AGG + chr5.3:32843832-32843851 None:intergenic 40.0%
TGGGAAGTTCTATGTTTGTG+TGG - chr5.3:32844237-32844256 MS.gene054713:CDS 40.0%
TTCTCTTCAAGTGGTATCTG+TGG + chr5.3:32844293-32844312 None:intergenic 40.0%
! CTTGAGTGTCATGCTCATTT+TGG - chr5.3:32843783-32843802 MS.gene054713:CDS 40.0%
! GAAGAAACTGGCCTAAGTAA+AGG + chr5.3:32844083-32844102 None:intergenic 40.0%
GAAGCTATTAGAGAGTGAGG+AGG + chr5.3:32843703-32843722 None:intergenic 45.0%
GACGAAGCTATTAGAGAGTG+AGG + chr5.3:32843706-32843725 None:intergenic 45.0%
GCAGCCCATTAGATTGGTAT+TGG - chr5.3:32843856-32843875 MS.gene054713:CDS 45.0%
TTGTGTGCCTTCTCTTCAAG+TGG + chr5.3:32844302-32844321 None:intergenic 45.0%
! GAAACTGGCCTAAGTAAAGG+AGG + chr5.3:32844080-32844099 None:intergenic 45.0%
CAACTCGCAGCCCATTAGAT+TGG - chr5.3:32843850-32843869 MS.gene054713:CDS 50.0%


Chromosome Type Strat End Strand Name
chr5.3 gene 32843621 32844406 32843621 ID=MS.gene054713
chr5.3 mRNA 32843621 32844406 32843621 ID=MS.gene054713.t1;Parent=MS.gene054713
chr5.3 exon 32843621 32844406 32843621 ID=MS.gene054713.t1.exon1;Parent=MS.gene054713.t1
chr5.3 CDS 32843621 32844406 32843621 ID=cds.MS.gene054713.t1;Parent=MS.gene054713.t1
Gene Sequence

>MS.gene054713

ATGGGTTTTCAAGGGCCTGATATTGAAAAGTTCATTCATTTCCTCTCAATATGTATCCTTGCTTTCATGAATGAAGGCTCCTCCTCACTCTCTAATAGCTTCGTCAATGAACAATGGTCATGGGGTCTAAGTATTCCACTAAGTTTATACTTCAAATTTGCACTTGAGTGTCATGCTCATTTTGGAATCTTTGTTGGGATTGGAGTACCCTCTTTAGTTTATTGGGGTGCAACTCGCAGCCCATTAGATTGGTATTGGGAAACAGTGATCAAATTCAATGTTGAAATTTTGAAAACTGGTTTCTTCAACCTTGTTATTGGATTGCTACATTTAGTTGTCTCGAAATTGCTCAATCGTTTAATTGAGTGGATACATGATCGTAAGATTAAACTCAAAGGGTTGGATTTTCTTTTCCCTAAGAAAAATTCAGAGTTGGAAAATTCAACCTTTTACGAACCTCCTTTACTTAGGCCAGTTTCTTCTCAAGACATAGTATTGAAACTTGTTGAAGCACTTATTTCCTTATTCTTTAATGGAAATGTATTGCTTTTAAGTTGGTGTTTTTCTGGACAGATTTACACACATGATGTTGGTAAAGCTATATCTTGGGTTAGTTTGGGAAGTTCTATGTTTGTGTGGATTAAGGCTTTACTTAAGCTAATGAAAATGCCACAGATACCACTTGAAGAGAAGGCACACAAGATTGACAAGAATCAAAATTGTTCTATAGATGACAAGAAAGAGTCCAAGGATCAAGAACTAGTTACATGCTCATTAGAATGTTGA

Protein sequence

>MS.gene054713.t1

MGFQGPDIEKFIHFLSICILAFMNEGSSSLSNSFVNEQWSWGLSIPLSLYFKFALECHAHFGIFVGIGVPSLVYWGATRSPLDWYWETVIKFNVEILKTGFFNLVIGLLHLVVSKLLNRLIEWIHDRKIKLKGLDFLFPKKNSELENSTFYEPPLLRPVSSQDIVLKLVEALISLFFNGNVLLLSWCFSGQIYTHDVGKAISWVSLGSSMFVWIKALLKLMKMPQIPLEEKAHKIDKNQNCSIDDKKESKDQELVTCSLEC