Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44790.t1 | XP_013447843.1 | 87.8 | 255 | 26 | 1 | 1 | 255 | 1 | 250 | 1.30E-114 | 422.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44790.t1 | A2XID3 | 69.7 | 195 | 57 | 2 | 62 | 255 | 47 | 240 | 5.4e-72 | 272.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44790.t1 | A0A072TWG0 | 87.8 | 255 | 26 | 1 | 1 | 255 | 1 | 250 | 9.7e-115 | 422.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene056694 | MS.gene44790 | 0.8056 | 1.23E-49 | -1.69E-46 |
| MS.gene061044 | MS.gene44790 | 0.82089 | 5.40E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44790.t1 | MTR_7g417750 | 92.549 | 255 | 14 | 1 | 1 | 255 | 1 | 250 | 1.97e-174 | 480 |
| MS.gene44790.t1 | MTR_4g069020 | 76.834 | 259 | 54 | 4 | 1 | 255 | 1 | 257 | 8.51e-142 | 398 |
| MS.gene44790.t1 | MTR_5g053950 | 74.468 | 188 | 47 | 1 | 68 | 255 | 66 | 252 | 1.32e-103 | 301 |
| MS.gene44790.t1 | MTR_6g017155 | 61.569 | 255 | 92 | 3 | 1 | 255 | 1 | 249 | 1.88e-103 | 300 |
| MS.gene44790.t1 | MTR_6g017165 | 62.869 | 237 | 82 | 4 | 1 | 237 | 1 | 231 | 2.15e-100 | 292 |
| MS.gene44790.t1 | MTR_8g062760 | 79.661 | 118 | 14 | 1 | 1 | 118 | 1 | 108 | 7.41e-59 | 182 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44790.t1 | AT1G13280 | 64.894 | 188 | 63 | 3 | 70 | 255 | 68 | 254 | 2.99e-85 | 254 |
| MS.gene44790.t1 | AT3G25780 | 60.664 | 211 | 68 | 5 | 47 | 255 | 61 | 258 | 2.19e-83 | 250 |
| MS.gene44790.t1 | AT3G25770 | 59.314 | 204 | 74 | 4 | 58 | 255 | 53 | 253 | 4.17e-82 | 246 |
| MS.gene44790.t1 | AT3G25760 | 49.808 | 261 | 118 | 6 | 1 | 255 | 1 | 254 | 2.33e-80 | 242 |
Find 43 sgRNAs with CRISPR-Local
Find 86 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCAATCTACAGTTTCCATTT+TGG | 0.179928 | 7.4:+81760407 | MS.gene44790:CDS |
| GAGCCTCATGCTGTCATTGC+TGG | 0.269913 | 7.4:+81760680 | MS.gene44790:CDS |
| CTAGCTGTGACTGGTGGTTC+TGG | 0.279570 | 7.4:+81760488 | MS.gene44790:CDS |
| TGAATGCATTGTTCATCTTT+AGG | 0.298780 | 7.4:-81759565 | None:intergenic |
| TCCTCAGGATAGGTCAAATA+TGG | 0.373814 | 7.4:-81760458 | None:intergenic |
| AAACGTATTGGAATAACAAC+TGG | 0.388638 | 7.4:+81760332 | MS.gene44790:CDS |
| GATTTGCCTCAAGAGCTTCT+TGG | 0.394018 | 7.4:+81760602 | MS.gene44790:CDS |
| AGTGACCATAGTCACCAAAA+TGG | 0.394121 | 7.4:-81760421 | None:intergenic |
| ACAGCAGGAGAAGGCTCAAC+AGG | 0.409306 | 7.4:-81760629 | None:intergenic |
| TTGCTTAATCTCAAGTAAGC+TGG | 0.428592 | 7.4:-81759812 | None:intergenic |
| AACAAAAGTGTCAATTCTCT+TGG | 0.448790 | 7.4:+81759833 | MS.gene44790:CDS |
| TGTCAGCATACTCATTTGCT+TGG | 0.464345 | 7.4:-81759637 | None:intergenic |
| AAGGGTGTGAAAGCAGATGA+GGG | 0.471406 | 7.4:-81759512 | None:intergenic |
| GCAGGAGAAGCTTCAACAGC+AGG | 0.485103 | 7.4:-81760644 | None:intergenic |
| GGCTCAAGAGCCTTAGCAGC+AGG | 0.487676 | 7.4:-81760662 | None:intergenic |
| TGAAGCTTCTCCTGCTGCTA+AGG | 0.489670 | 7.4:+81760652 | MS.gene44790:CDS |
| TGCTTAATCTCAAGTAAGCT+GGG | 0.493235 | 7.4:-81759811 | None:intergenic |
| GAAGGGTGTGAAAGCAGATG+AGG | 0.507693 | 7.4:-81759513 | None:intergenic |
| GTTGGATTAACAAAGGAAGA+TGG | 0.515342 | 7.4:-81759455 | None:intergenic |
| TATGGTCACTTAGCAGTTCA+AGG | 0.517659 | 7.4:+81760434 | MS.gene44790:CDS |
| CAACACATTGCAGAAAAGAA+AGG | 0.527550 | 7.4:+81760371 | MS.gene44790:CDS |
| AGATGAGTTTGATGAAGTGA+AGG | 0.532217 | 7.4:-81759531 | None:intergenic |
| GGGGTGGTGGCAAAAGCAGT+TGG | 0.556218 | 7.4:-81759473 | None:intergenic |
| TAGATAAGTGTCCTCAGGAT+AGG | 0.557748 | 7.4:-81760468 | None:intergenic |
| ACAGCTAGATAAGTGTCCTC+AGG | 0.560526 | 7.4:-81760473 | None:intergenic |
| AATGTGTAGAAAATCTTGAA+AGG | 0.563806 | 7.4:-81760560 | None:intergenic |
| TGAATTATGTAGTTATACAC+AGG | 0.568803 | 7.4:+81760299 | MS.gene44790:intron |
| GAAGCTTCAACAGCAGGAGA+AGG | 0.572761 | 7.4:-81760638 | None:intergenic |
| GACACTTATCTAGCTGTGAC+TGG | 0.577356 | 7.4:+81760479 | MS.gene44790:CDS |
| TCAAGTAAGCTGGGCTACCA+CGG | 0.602620 | 7.4:-81759802 | None:intergenic |
| TCTCAAAGCATAGCTTGAAG+AGG | 0.618632 | 7.4:-81759426 | None:intergenic |
| GATGAGTTTGATGAAGTGAA+GGG | 0.622372 | 7.4:-81759530 | None:intergenic |
| GCAAATGAGTATGCTGACAC+TGG | 0.626985 | 7.4:+81759641 | MS.gene44790:CDS |
| GGTTTACCAAGAAGCTCTTG+AGG | 0.629043 | 7.4:-81760608 | None:intergenic |
| GGGTGTGAAAGCAGATGAGG+GGG | 0.634086 | 7.4:-81759510 | None:intergenic |
| AAACCAGCAATGACAGCATG+AGG | 0.634146 | 7.4:-81760683 | None:intergenic |
| AAAAGCAGTTGGATTAACAA+AGG | 0.635467 | 7.4:-81759462 | None:intergenic |
| ACTTATCTAGCTGTGACTGG+TGG | 0.640066 | 7.4:+81760482 | MS.gene44790:CDS |
| AGGGTGTGAAAGCAGATGAG+GGG | 0.653243 | 7.4:-81759511 | None:intergenic |
| GAGCTTAATGAGAGGGACCG+TGG | 0.672691 | 7.4:+81759785 | MS.gene44790:CDS |
| TGTGTATGAGCTTAATGAGA+GGG | 0.682673 | 7.4:+81759778 | MS.gene44790:CDS |
| ACCATATTTGACCTATCCTG+AGG | 0.683765 | 7.4:+81760457 | MS.gene44790:CDS |
| ATGTGTATGAGCTTAATGAG+AGG | 0.762359 | 7.4:+81759777 | MS.gene44790:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ACCTAATTATAAAAAATTAA+GGG | + | chr7.4:81760004-81760023 | MS.gene44790:intron | 10.0% |
| !!! | AATTGTATTTTTTTTTTCTT+TGG | + | chr7.4:81760093-81760112 | MS.gene44790:intron | 10.0% |
| !!! | ATTATTGATAGTTTTATTAT+CGG | + | chr7.4:81759935-81759954 | MS.gene44790:intron | 10.0% |
| !! | ATCACAAAAATAAAATATAG+AGG | - | chr7.4:81759911-81759930 | None:intergenic | 15.0% |
| !! | CATAAATCATAAACAAAATA+AGG | - | chr7.4:81760174-81760193 | None:intergenic | 15.0% |
| !! | GACCTAATTATAAAAAATTA+AGG | + | chr7.4:81760003-81760022 | MS.gene44790:intron | 15.0% |
| !! | TTATTTAAATGACACAAAAT+GGG | - | chr7.4:81760221-81760240 | None:intergenic | 15.0% |
| !! | TTTATTTAAATGACACAAAA+TGG | - | chr7.4:81760222-81760241 | None:intergenic | 15.0% |
| !!! | ACCCTTAATTTTTTATAATT+AGG | - | chr7.4:81760008-81760027 | None:intergenic | 15.0% |
| !!! | ATCCATAAACAATTTTTTAA+TGG | - | chr7.4:81760040-81760059 | None:intergenic | 15.0% |
| !! | CTCCATTAAAAAATTGTTTA+TGG | + | chr7.4:81760035-81760054 | MS.gene44790:intron | 20.0% |
| !!! | TTTAGAAAAGCATGTATATT+TGG | - | chr7.4:81759684-81759703 | None:intergenic | 20.0% |
| !!! | TTTCTACACATTTTATTTGA+GGG | + | chr7.4:81760571-81760590 | MS.gene44790:CDS | 20.0% |
| !!! | TTTTCTACACATTTTATTTG+AGG | + | chr7.4:81760570-81760589 | MS.gene44790:CDS | 20.0% |
| !!! | TTTTTTACTTTGTTACTGAA+GGG | - | chr7.4:81759869-81759888 | None:intergenic | 20.0% |
| !!! | TTTTTTTACTTTGTTACTGA+AGG | - | chr7.4:81759870-81759889 | None:intergenic | 20.0% |
| ! | AATGTGTAGAAAATCTTGAA+AGG | - | chr7.4:81760563-81760582 | None:intergenic | 25.0% |
| ! | TGAATTATGTAGTTATACAC+AGG | + | chr7.4:81760299-81760318 | MS.gene44790:intron | 25.0% |
| !! | TTATTCCAATACGTTTTTGT+AGG | - | chr7.4:81760328-81760347 | None:intergenic | 25.0% |
| !! | TTTTTACTTTGTTACTGAAG+GGG | - | chr7.4:81759868-81759887 | None:intergenic | 25.0% |
| !!! | ATTTTATTTGAGGGGTATTA+AGG | + | chr7.4:81760580-81760599 | MS.gene44790:CDS | 25.0% |
| !!! | TTCCATGAACTATTTTAGTA+GGG | + | chr7.4:81759981-81760000 | MS.gene44790:intron | 25.0% |
| !!! | TTCTACACATTTTATTTGAG+GGG | + | chr7.4:81760572-81760591 | MS.gene44790:CDS | 25.0% |
| AAAAGCAGTTGGATTAACAA+AGG | - | chr7.4:81759465-81759484 | None:intergenic | 30.0% | |
| AAACGTATTGGAATAACAAC+TGG | + | chr7.4:81760332-81760351 | MS.gene44790:CDS | 30.0% | |
| CTAAAATAGTTCATGGAAGT+TGG | - | chr7.4:81759979-81759998 | None:intergenic | 30.0% | |
| TGAATGCATTGTTCATCTTT+AGG | - | chr7.4:81759568-81759587 | None:intergenic | 30.0% | |
| !! | AACAAAAGTGTCAATTCTCT+TGG | + | chr7.4:81759833-81759852 | MS.gene44790:CDS | 30.0% |
| !!! | CTTCCATGAACTATTTTAGT+AGG | + | chr7.4:81759980-81759999 | MS.gene44790:intron | 30.0% |
| !!! | GGAATTTTTGAAGGTGTTAA+AGG | + | chr7.4:81760509-81760528 | MS.gene44790:CDS | 30.0% |
| AGATGAGTTTGATGAAGTGA+AGG | - | chr7.4:81759534-81759553 | None:intergenic | 35.0% | |
| ATGTGTATGAGCTTAATGAG+AGG | + | chr7.4:81759777-81759796 | MS.gene44790:CDS | 35.0% | |
| CAACACATTGCAGAAAAGAA+AGG | + | chr7.4:81760371-81760390 | MS.gene44790:CDS | 35.0% | |
| GATGAGTTTGATGAAGTGAA+GGG | - | chr7.4:81759533-81759552 | None:intergenic | 35.0% | |
| GTCCCTACTAAAATAGTTCA+TGG | - | chr7.4:81759986-81760005 | None:intergenic | 35.0% | |
| GTTGGATTAACAAAGGAAGA+TGG | - | chr7.4:81759458-81759477 | None:intergenic | 35.0% | |
| TGCTTAATCTCAAGTAAGCT+GGG | - | chr7.4:81759814-81759833 | None:intergenic | 35.0% | |
| TGTGTATGAGCTTAATGAGA+GGG | + | chr7.4:81759778-81759797 | MS.gene44790:CDS | 35.0% | |
| TTGCTTAATCTCAAGTAAGC+TGG | - | chr7.4:81759815-81759834 | None:intergenic | 35.0% | |
| ! | CTTTCTTTTCTGCAATGTGT+TGG | - | chr7.4:81760373-81760392 | None:intergenic | 35.0% |
| ! | GCAATCTACAGTTTCCATTT+TGG | + | chr7.4:81760407-81760426 | MS.gene44790:CDS | 35.0% |
| !! | AGTTTCCATTTTGGTGACTA+TGG | + | chr7.4:81760416-81760435 | MS.gene44790:CDS | 35.0% |
| ACACAAAATGGGACTACTAC+GGG | - | chr7.4:81760210-81760229 | None:intergenic | 40.0% | |
| ACCATATTTGACCTATCCTG+AGG | + | chr7.4:81760457-81760476 | MS.gene44790:CDS | 40.0% | |
| AGTGACCATAGTCACCAAAA+TGG | - | chr7.4:81760424-81760443 | None:intergenic | 40.0% | |
| GACACAAAATGGGACTACTA+CGG | - | chr7.4:81760211-81760230 | None:intergenic | 40.0% | |
| GGTGACCTACAAAAACGTAT+TGG | + | chr7.4:81760320-81760339 | MS.gene44790:CDS | 40.0% | |
| TAGATAAGTGTCCTCAGGAT+AGG | - | chr7.4:81760471-81760490 | None:intergenic | 40.0% | |
| TATGGTCACTTAGCAGTTCA+AGG | + | chr7.4:81760434-81760453 | MS.gene44790:CDS | 40.0% | |
| TCCTCAGGATAGGTCAAATA+TGG | - | chr7.4:81760461-81760480 | None:intergenic | 40.0% | |
| TCTCAAAGCATAGCTTGAAG+AGG | - | chr7.4:81759429-81759448 | None:intergenic | 40.0% | |
| TGTCAGCATACTCATTTGCT+TGG | - | chr7.4:81759640-81759659 | None:intergenic | 40.0% | |
| !!! | CACACAATCTAGCTTTTCTG+CGG | + | chr7.4:81759586-81759605 | MS.gene44790:CDS | 40.0% |
| !!! | GGTGGTTCTGGAATTTTTGA+AGG | + | chr7.4:81760500-81760519 | MS.gene44790:CDS | 40.0% |
| AAACCAGCAATGACAGCATG+AGG | - | chr7.4:81760686-81760705 | None:intergenic | 45.0% | |
| ACAGCTAGATAAGTGTCCTC+AGG | - | chr7.4:81760476-81760495 | None:intergenic | 45.0% | |
| ACTTATCTAGCTGTGACTGG+TGG | + | chr7.4:81760482-81760501 | MS.gene44790:CDS | 45.0% | |
| CACAAAATGGGACTACTACG+GGG | - | chr7.4:81760209-81760228 | None:intergenic | 45.0% | |
| GACACTTATCTAGCTGTGAC+TGG | + | chr7.4:81760479-81760498 | MS.gene44790:CDS | 45.0% | |
| GATTTGCCTCAAGAGCTTCT+TGG | + | chr7.4:81760602-81760621 | MS.gene44790:CDS | 45.0% | |
| GCAAATGAGTATGCTGACAC+TGG | + | chr7.4:81759641-81759660 | MS.gene44790:CDS | 45.0% | |
| GGTTTACCAAGAAGCTCTTG+AGG | - | chr7.4:81760611-81760630 | None:intergenic | 45.0% | |
| ! | AAGGGTGTGAAAGCAGATGA+GGG | - | chr7.4:81759515-81759534 | None:intergenic | 45.0% |
| !!! | ACAATCTAGCTTTTCTGCGG+TGG | + | chr7.4:81759589-81759608 | MS.gene44790:CDS | 45.0% |
| !!! | TAAAATTTTCAAATAATTTA+AGG | + | chr7.4:81760131-81760150 | MS.gene44790:intron | 5.0% |
| !!! | TTATTTGAAAATTTTAAAAT+AGG | - | chr7.4:81760127-81760146 | None:intergenic | 5.0% |
| AGGGTGTGAAAGCAGATGAG+GGG | - | chr7.4:81759514-81759533 | None:intergenic | 50.0% | |
| GAAGCTTCAACAGCAGGAGA+AGG | - | chr7.4:81760641-81760660 | None:intergenic | 50.0% | |
| TCAAGTAAGCTGGGCTACCA+CGG | - | chr7.4:81759805-81759824 | None:intergenic | 50.0% | |
| TGAAGCTTCTCCTGCTGCTA+AGG | + | chr7.4:81760652-81760671 | MS.gene44790:CDS | 50.0% | |
| ! | AGGGGGTGAGGAAGGATTTT+GGG | - | chr7.4:81759496-81759515 | None:intergenic | 50.0% |
| ! | GAAGGGTGTGAAAGCAGATG+AGG | - | chr7.4:81759516-81759535 | None:intergenic | 50.0% |
| ACAGCAGGAGAAGGCTCAAC+AGG | - | chr7.4:81760632-81760651 | None:intergenic | 55.0% | |
| CTAGCTGTGACTGGTGGTTC+TGG | + | chr7.4:81760488-81760507 | MS.gene44790:CDS | 55.0% | |
| GAGCCTCATGCTGTCATTGC+TGG | + | chr7.4:81760680-81760699 | MS.gene44790:CDS | 55.0% | |
| GAGCTTAATGAGAGGGACCG+TGG | + | chr7.4:81759785-81759804 | MS.gene44790:CDS | 55.0% | |
| GCAGGAGAAGCTTCAACAGC+AGG | - | chr7.4:81760647-81760666 | None:intergenic | 55.0% | |
| GGGTGTGAAAGCAGATGAGG+GGG | - | chr7.4:81759513-81759532 | None:intergenic | 55.0% | |
| TGAAAGCAGATGAGGGGGTG+AGG | - | chr7.4:81759508-81759527 | None:intergenic | 55.0% | |
| ! | GAGGGGGTGAGGAAGGATTT+TGG | - | chr7.4:81759497-81759516 | None:intergenic | 55.0% |
| ! | GGGGGTGAGGAAGGATTTTG+GGG | - | chr7.4:81759495-81759514 | None:intergenic | 55.0% |
| !! | GGTGAGGAAGGATTTTGGGG+TGG | - | chr7.4:81759492-81759511 | None:intergenic | 55.0% |
| !!! | GAGGAAGGATTTTGGGGTGG+TGG | - | chr7.4:81759489-81759508 | None:intergenic | 55.0% |
| AGCAGATGAGGGGGTGAGGA+AGG | - | chr7.4:81759504-81759523 | None:intergenic | 60.0% | |
| GGCTCAAGAGCCTTAGCAGC+AGG | - | chr7.4:81760665-81760684 | None:intergenic | 60.0% | |
| ! | GGGGTGGTGGCAAAAGCAGT+TGG | - | chr7.4:81759476-81759495 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 81759422 | 81760715 | 81759422 | ID=MS.gene44790 |
| chr7.4 | mRNA | 81759422 | 81760715 | 81759422 | ID=MS.gene44790.t1;Parent=MS.gene44790 |
| chr7.4 | exon | 81759422 | 81759662 | 81759422 | ID=MS.gene44790.t1.exon1;Parent=MS.gene44790.t1 |
| chr7.4 | CDS | 81759422 | 81759662 | 81759422 | ID=cds.MS.gene44790.t1;Parent=MS.gene44790.t1 |
| chr7.4 | exon | 81759759 | 81759880 | 81759759 | ID=MS.gene44790.t1.exon2;Parent=MS.gene44790.t1 |
| chr7.4 | CDS | 81759759 | 81759880 | 81759759 | ID=cds.MS.gene44790.t1;Parent=MS.gene44790.t1 |
| chr7.4 | exon | 81760311 | 81760715 | 81760311 | ID=MS.gene44790.t1.exon3;Parent=MS.gene44790.t1 |
| chr7.4 | CDS | 81760311 | 81760715 | 81760311 | ID=cds.MS.gene44790.t1;Parent=MS.gene44790.t1 |
| Gene Sequence |
| Protein sequence |
