AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene45453

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene45453.t1 MTR_4g071400 97.468 79 2 0 185 263 43 121 6.28e-47 154
MS.gene45453.t1 MTR_3g095940 72.222 54 15 0 125 178 90 143 1.43e-20 86.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene45453.t1 AT1G10240 56.731 104 45 0 1 104 291 394 3.54e-37 140
MS.gene45453.t1 AT1G10240 45.882 85 45 1 99 183 470 553 8.73e-18 84.3
MS.gene45453.t1 AT1G10240 56.731 104 45 0 1 104 291 394 3.54e-37 140
MS.gene45453.t1 AT1G10240 45.882 85 45 1 99 183 470 553 8.73e-18 84.3
MS.gene45453.t1 AT5G28530 47.525 101 53 0 1 101 306 406 2.38e-29 118
MS.gene45453.t1 AT5G28530 40.000 75 45 0 107 181 491 565 2.39e-12 67.8
MS.gene45453.t1 AT5G28530 47.525 101 53 0 1 101 306 406 2.63e-29 118
MS.gene45453.t1 AT5G28530 44.444 63 35 0 119 181 499 561 1.50e-11 65.5
MS.gene45453.t1 AT5G28530 47.525 101 53 0 1 101 306 406 2.63e-29 118
MS.gene45453.t1 AT5G28530 44.444 63 35 0 119 181 499 561 1.50e-11 65.5
MS.gene45453.t1 AT5G28530 48.913 92 47 0 10 101 252 343 3.60e-26 108
MS.gene45453.t1 AT5G28530 40.000 75 45 0 107 181 428 502 2.39e-12 67.8
MS.gene45453.t1 AT5G28530 48.913 92 47 0 10 101 252 343 3.97e-26 108
MS.gene45453.t1 AT5G28530 44.444 63 35 0 119 181 436 498 1.58e-11 65.5
MS.gene45453.t1 AT4G15090 34.711 121 74 2 1 121 289 404 5.96e-21 93.6
MS.gene45453.t1 AT4G15090 34.711 121 74 2 1 121 289 404 5.96e-21 93.6
MS.gene45453.t1 AT4G15090 34.711 121 74 2 1 121 289 404 5.96e-21 93.6
MS.gene45453.t1 AT4G15090 34.711 121 74 2 1 121 289 404 5.96e-21 93.6
MS.gene45453.t1 AT4G15090 34.711 121 74 2 1 121 289 404 5.96e-21 93.6
MS.gene45453.t1 AT4G38180 34.951 103 66 1 1 103 313 414 5.03e-15 75.9
MS.gene45453.t1 AT1G76320 39.024 82 50 0 1 82 226 307 2.79e-14 73.9
MS.gene45453.t1 AT1G76320 39.024 82 50 0 1 82 226 307 2.79e-14 73.9
MS.gene45453.t1 AT1G76320 39.024 82 50 0 1 82 226 307 2.87e-14 73.6
MS.gene45453.t1 AT3G22170 30.097 103 71 1 1 103 298 399 2.88e-14 73.9
MS.gene45453.t1 AT3G22170 30.097 103 71 1 1 103 298 399 2.88e-14 73.9
MS.gene45453.t1 AT1G76320 39.024 82 50 0 1 82 226 307 3.15e-14 73.6
MS.gene45453.t1 AT2G32250 28.713 101 71 1 1 101 233 332 1.92e-13 71.2
MS.gene45453.t1 AT2G32250 28.713 101 71 1 1 101 233 332 1.92e-13 71.2
MS.gene45453.t1 AT2G32250 28.713 101 71 1 1 101 233 332 2.02e-13 71.2
MS.gene45453.t1 AT2G32250 28.713 101 71 1 1 101 233 332 2.03e-13 71.2
MS.gene45453.t1 AT2G32250 28.713 101 71 1 1 101 233 332 2.03e-13 71.2
MS.gene45453.t1 AT2G32250 28.713 101 71 1 1 101 233 332 2.03e-13 71.2
MS.gene45453.t1 AT2G32250 28.713 101 71 1 1 101 192 291 2.46e-13 70.9
MS.gene45453.t1 AT2G32250 28.713 101 71 1 1 101 192 291 2.50e-13 70.9
MS.gene45453.t1 AT2G27110 32.911 79 53 0 1 79 260 338 7.69e-12 66.6
MS.gene45453.t1 AT2G27110 32.911 79 53 0 1 79 260 338 7.69e-12 66.6
MS.gene45453.t1 AT2G27110 32.911 79 53 0 1 79 115 193 9.32e-12 66.2
MS.gene45453.t1 AT3G06250 28.846 104 68 2 1 103 389 487 1.25e-11 65.9
MS.gene45453.t1 AT3G06250 28.846 104 68 2 1 103 389 487 1.25e-11 65.9
MS.gene45453.t1 AT3G06250 28.846 104 68 2 1 103 389 487 1.25e-11 65.9

Find 49 sgRNAs with CRISPR-Local

Find 92 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCAATTAAAACTTACTTATT+AGG 0.177601 1.2:-44791224 None:intergenic
ATGAGATGCCTCTTGGAATA+TGG 0.196486 1.2:+44789673 None:intergenic
AACGTCACAATCATCATTAT+TGG 0.207637 1.2:-44791024 None:intergenic
ATCACATGTTCTTGAGTTTC+AGG 0.210618 1.2:-44790981 None:intergenic
CTCATGTTGAAGCAACTTAA+AGG 0.248230 1.2:-44790547 None:intergenic
TTCCTTCGCATGGGCTATAA+AGG 0.299718 1.2:+44789764 MS.gene45453:CDS
AATATCATCATAAAGATAAA+AGG 0.310779 1.2:+44790954 MS.gene45453:CDS
TCAAGAACATGTGATTGATT+TGG 0.314506 1.2:+44790989 MS.gene45453:CDS
GCAGCTTCAACCCTTCCTTT+TGG 0.339103 1.2:-44791095 None:intergenic
GCAATTTCTTCTTTAATTGA+AGG 0.360580 1.2:-44789936 None:intergenic
ATGACAACGACGAGGCTTCT+TGG 0.366012 1.2:-44791117 None:intergenic
TCAGTCTCTAATTCGATGTT+TGG 0.372448 1.2:+44790875 MS.gene45453:intron
TGAGATGCCTCTTGGAATAT+GGG 0.433904 1.2:+44789674 None:intergenic
TATACGACCACCATCAACTT+TGG 0.437731 1.2:-44790637 None:intergenic
CTTTAAACATGAGTTTCATA+AGG 0.439926 1.2:+44790058 MS.gene45453:CDS
GACGAGGCATGTTCTTCAAT+AGG 0.443666 1.2:-44790507 None:intergenic
TGATTAGTTCCACCACAATC+TGG 0.446933 1.2:-44791143 None:intergenic
AACCGTTATGAGATGCCTCT+TGG 0.448749 1.2:+44789666 None:intergenic
AAAGACAAGTGAACGAGAAC+AGG 0.453486 1.2:+44790911 MS.gene45453:CDS
AAAACAAAAGGTCGGCCAAA+AGG 0.495104 1.2:+44791065 MS.gene45453:CDS
TTGATAGGATCATGAAGGGT+TGG 0.498564 1.2:+44791311 MS.gene45453:intron
TGAAGCAACTTAAAGGCATA+AGG 0.499495 1.2:-44790540 None:intergenic
AGAAGATTTCTTCCTTCGCA+TGG 0.503824 1.2:+44789754 MS.gene45453:CDS
CGTTGTCATGTTCCAGATTG+TGG 0.504732 1.2:+44791131 MS.gene45453:CDS
CCAAAAGGATCAAGACCAAA+AGG 0.514192 1.2:+44791080 MS.gene45453:CDS
GTCAAAATTAGAAATCAAGC+TGG 0.517568 1.2:+44790429 MS.gene45453:CDS
TTTAATTGAAGGATCTTGAT+CGG 0.518039 1.2:-44789925 None:intergenic
TGGGTTGGTGTTGATAATCA+TGG 0.529258 1.2:+44789693 MS.gene45453:CDS
CAACATCATACCAAAGTTGA+TGG 0.538288 1.2:+44790627 MS.gene45453:CDS
GAAGATTTCTTCCTTCGCAT+GGG 0.556681 1.2:+44789755 MS.gene45453:CDS
GTGCTTTGATAGGATCATGA+AGG 0.556862 1.2:+44791306 MS.gene45453:intron
GGAAAATGTGTTGTAATCTG+AGG 0.568974 1.2:-44790486 None:intergenic
TAGAAATCAAGCTGGAGAAG+AGG 0.577533 1.2:+44790437 MS.gene45453:CDS
AAGGATCAAGACCAAAAGGA+AGG 0.577608 1.2:+44791084 MS.gene45453:CDS
TTGTCGCAAAGCTGTCAAGT+TGG 0.589381 1.2:+44790003 MS.gene45453:CDS
AATTAAAGAAGAAATTGCAA+TGG 0.594953 1.2:+44789941 MS.gene45453:CDS
TCATCATATCTCGAACCTAG+TGG 0.597376 1.2:-44790035 None:intergenic
TACCTTTATAGCCCATGCGA+AGG 0.601051 1.2:-44789766 None:intergenic
ACATGTGATTGATTTGGAAG+CGG 0.601602 1.2:+44790995 MS.gene45453:CDS
ATGCCTCTTGGAATATGGGT+TGG 0.616141 1.2:+44789678 MS.gene45453:CDS
AGGATCAAGACCAAAAGGAA+GGG 0.629187 1.2:+44791085 MS.gene45453:CDS
TACTTATTAGGAGATTGAGA+AGG 0.635742 1.2:-44791212 None:intergenic
TGTCATGTTCCAGATTGTGG+TGG 0.637809 1.2:+44791134 MS.gene45453:CDS
GCAGAAATTGAAGCTGACAA+TGG 0.650940 1.2:+44790591 MS.gene45453:CDS
ACACCAACCCATATTCCAAG+AGG 0.676376 1.2:-44789681 None:intergenic
TGCTTTGATAGGATCATGAA+GGG 0.676443 1.2:+44791307 MS.gene45453:intron
CATCATACCAAAGTTGATGG+TGG 0.683172 1.2:+44790630 MS.gene45453:CDS
ATAAGGTGTGAGTATAGACG+AGG 0.720548 1.2:-44790523 None:intergenic
TCTGGAACATGACAACGACG+AGG 0.797744 1.2:-44791125 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATATCATCATAAAGATAAA+AGG + chr1.2:44790954-44790973 MS.gene45453:CDS 15.0%
!!! TAATATTAACTGTGTTTTTA+TGG + chr1.2:44791284-44791303 MS.gene45453:intron 15.0%
!! AATTAAAGAAGAAATTGCAA+TGG + chr1.2:44789941-44789960 MS.gene45453:CDS 20.0%
!! CCAATTAAAACTTACTTATT+AGG - chr1.2:44791227-44791246 None:intergenic 20.0%
!!! CATGGAAATTCAATTTTTTT+TGG + chr1.2:44789711-44789730 MS.gene45453:CDS 20.0%
!!! CCTAATAAGTAAGTTTTAAT+TGG + chr1.2:44791224-44791243 MS.gene45453:intron 20.0%
!!! TGATTGTTATATTTTTGTGT+AGG + chr1.2:44790395-44790414 MS.gene45453:intron 20.0%
!!! TTATTACATGACTAGATTTT+GGG - chr1.2:44790827-44790846 None:intergenic 20.0%
!!! TTTATTACATGACTAGATTT+TGG - chr1.2:44790828-44790847 None:intergenic 20.0%
! CTTTAAACATGAGTTTCATA+AGG + chr1.2:44790058-44790077 MS.gene45453:CDS 25.0%
! GCAATTTCTTCTTTAATTGA+AGG - chr1.2:44789939-44789958 None:intergenic 25.0%
! TCCAAACAAAAAGAGAAAAA+TGG + chr1.2:44791181-44791200 MS.gene45453:CDS 25.0%
! TTGTTTCAAAAACAAAAGGT+CGG + chr1.2:44791057-44791076 MS.gene45453:CDS 25.0%
! TTTAATTGAAGGATCTTGAT+CGG - chr1.2:44789928-44789947 None:intergenic 25.0%
!! TGTAAAGAGTTTCAATTTTC+AGG + chr1.2:44790699-44790718 MS.gene45453:intron 25.0%
!!! AAAATCTGTCAAACTTGTTT+TGG - chr1.2:44790313-44790332 None:intergenic 25.0%
!!! TCCATTTTTCTCTTTTTGTT+TGG - chr1.2:44791185-44791204 None:intergenic 25.0%
!!! TGTTATATTTTTGTGTAGGT+TGG + chr1.2:44790399-44790418 MS.gene45453:intron 25.0%
AAAGTCCTTCAAATAAGCTA+AGG - chr1.2:44790201-44790220 None:intergenic 30.0%
AACGTCACAATCATCATTAT+TGG - chr1.2:44791027-44791046 None:intergenic 30.0%
CATGTAATAAACTGCAATGA+TGG + chr1.2:44790837-44790856 MS.gene45453:intron 30.0%
CGAATTAGAGACTGAAATTT+GGG - chr1.2:44790870-44790889 None:intergenic 30.0%
GTCAAAATTAGAAATCAAGC+TGG + chr1.2:44790429-44790448 MS.gene45453:CDS 30.0%
TACTTATTAGGAGATTGAGA+AGG - chr1.2:44791215-44791234 None:intergenic 30.0%
TCAAGAACATGTGATTGATT+TGG + chr1.2:44790989-44791008 MS.gene45453:CDS 30.0%
TCGAATTAGAGACTGAAATT+TGG - chr1.2:44790871-44790890 None:intergenic 30.0%
! ATTTTCCTTTCACAAAAGCT+AGG - chr1.2:44789887-44789906 None:intergenic 30.0%
! CAAAACATGCATTTTGCATA+TGG + chr1.2:44789976-44789995 MS.gene45453:CDS 30.0%
! CAAAATATCTTCCTTTTCGA+TGG + chr1.2:44790781-44790800 MS.gene45453:intron 30.0%
! CAAAATGCATGTTTTGTGTT+TGG - chr1.2:44789972-44789991 None:intergenic 30.0%
! CTTTACCCATCGTAAATTTT+GGG + chr1.2:44790175-44790194 MS.gene45453:intron 30.0%
! GATATTTTCTAACTCTCTTG+TGG - chr1.2:44790940-44790959 None:intergenic 30.0%
! TAGATGATTTTGAACGAGAA+TGG + chr1.2:44790099-44790118 MS.gene45453:intron 30.0%
! TCTTTACCCATCGTAAATTT+TGG + chr1.2:44790174-44790193 MS.gene45453:intron 30.0%
! TGATCGGTAAGAATTGTTTT+TGG - chr1.2:44789912-44789931 None:intergenic 30.0%
! TTGGCATCTAAGAATTGTTT+TGG - chr1.2:44790294-44790313 None:intergenic 30.0%
!! CCTGTTGTTTCAAAAACAAA+AGG + chr1.2:44791053-44791072 MS.gene45453:CDS 30.0%
!! CGAAAAGGAAGATATTTTGA+TGG - chr1.2:44790780-44790799 None:intergenic 30.0%
!! TGTTTTTATGGTGCTTTGAT+AGG + chr1.2:44791296-44791315 MS.gene45453:intron 30.0%
!!! CCTTTTGTTTTTGAAACAAC+AGG - chr1.2:44791056-44791075 None:intergenic 30.0%
!!! TTGAAGGACTTTTTTTTTGC+TGG + chr1.2:44790209-44790228 MS.gene45453:intron 30.0%
AAAGTAATGGTCTCACAGTT+TGG + chr1.2:44790122-44790141 MS.gene45453:intron 35.0%
ATCACATGTTCTTGAGTTTC+AGG - chr1.2:44790984-44791003 None:intergenic 35.0%
CAACATCATACCAAAGTTGA+TGG + chr1.2:44790627-44790646 MS.gene45453:CDS 35.0%
CTCATGTTGAAGCAACTTAA+AGG - chr1.2:44790550-44790569 None:intergenic 35.0%
GGAAAATGTGTTGTAATCTG+AGG - chr1.2:44790489-44790508 None:intergenic 35.0%
TAAGGCCCAAAATTTACGAT+GGG - chr1.2:44790183-44790202 None:intergenic 35.0%
TCAGTCTCTAATTCGATGTT+TGG + chr1.2:44790875-44790894 MS.gene45453:intron 35.0%
TGAACGAGAATGGAAAGTAA+TGG + chr1.2:44790109-44790128 MS.gene45453:intron 35.0%
TGCTTTGATAGGATCATGAA+GGG + chr1.2:44791307-44791326 MS.gene45453:intron 35.0%
! TGAAGCAACTTAAAGGCATA+AGG - chr1.2:44790543-44790562 None:intergenic 35.0%
!! ACATGTGATTGATTTGGAAG+CGG + chr1.2:44790995-44791014 MS.gene45453:CDS 35.0%
!! AGTTTCCTAGCTTTTGTGAA+AGG + chr1.2:44789879-44789898 MS.gene45453:CDS 35.0%
!!! AGTTGGTTTTCTTTTCCACT+AGG + chr1.2:44790020-44790039 MS.gene45453:CDS 35.0%
AAAACAAAAGGTCGGCCAAA+AGG + chr1.2:44791065-44791084 MS.gene45453:CDS 40.0%
AAAGACAAGTGAACGAGAAC+AGG + chr1.2:44790911-44790930 MS.gene45453:CDS 40.0%
AAGGATCAAGACCAAAAGGA+AGG + chr1.2:44791084-44791103 MS.gene45453:CDS 40.0%
AATGGTCTCACAGTTTGGTT+TGG + chr1.2:44790127-44790146 MS.gene45453:intron 40.0%
AGAAGATTTCTTCCTTCGCA+TGG + chr1.2:44789754-44789773 MS.gene45453:CDS 40.0%
AGGATCAAGACCAAAAGGAA+GGG + chr1.2:44791085-44791104 MS.gene45453:CDS 40.0%
ATAAGGTGTGAGTATAGACG+AGG - chr1.2:44790526-44790545 None:intergenic 40.0%
CCAAAAGGATCAAGACCAAA+AGG + chr1.2:44791080-44791099 MS.gene45453:CDS 40.0%
CTAAGGCCCAAAATTTACGA+TGG - chr1.2:44790184-44790203 None:intergenic 40.0%
GAAGATTTCTTCCTTCGCAT+GGG + chr1.2:44789755-44789774 MS.gene45453:CDS 40.0%
GCAGAAATTGAAGCTGACAA+TGG + chr1.2:44790591-44790610 MS.gene45453:CDS 40.0%
GTGCTTTGATAGGATCATGA+AGG + chr1.2:44791306-44791325 MS.gene45453:intron 40.0%
TAGAAATCAAGCTGGAGAAG+AGG + chr1.2:44790437-44790456 MS.gene45453:CDS 40.0%
TAGAGACTGAAATTTGGGAG+AGG - chr1.2:44790865-44790884 None:intergenic 40.0%
TATACGACCACCATCAACTT+TGG - chr1.2:44790640-44790659 None:intergenic 40.0%
TCATCATATCTCGAACCTAG+TGG - chr1.2:44790038-44790057 None:intergenic 40.0%
TGGTGGTCGTATAGTAAGTT+TGG + chr1.2:44790647-44790666 MS.gene45453:intron 40.0%
TTGATAGGATCATGAAGGGT+TGG + chr1.2:44791311-44791330 MS.gene45453:intron 40.0%
TTGGGCCTTAGCTTATTTGA+AGG + chr1.2:44790193-44790212 MS.gene45453:intron 40.0%
TTTGCTGGAATGACAACAAC+AGG + chr1.2:44790224-44790243 MS.gene45453:intron 40.0%
! ATGGTCTCACAGTTTGGTTT+GGG + chr1.2:44790128-44790147 MS.gene45453:intron 40.0%
! TGATTAGTTCCACCACAATC+TGG - chr1.2:44791146-44791165 None:intergenic 40.0%
!! CATCATACCAAAGTTGATGG+TGG + chr1.2:44790630-44790649 MS.gene45453:CDS 40.0%
!! TGGGTTGGTGTTGATAATCA+TGG + chr1.2:44789693-44789712 MS.gene45453:CDS 40.0%
!!! CCTTTTGGTCTTGATCCTTT+TGG - chr1.2:44791083-44791102 None:intergenic 40.0%
ACACCAACCCATATTCCAAG+AGG - chr1.2:44789684-44789703 None:intergenic 45.0%
ATGCCTCTTGGAATATGGGT+TGG + chr1.2:44789678-44789697 MS.gene45453:CDS 45.0%
CGTTGTCATGTTCCAGATTG+TGG + chr1.2:44791131-44791150 MS.gene45453:CDS 45.0%
CTTTCTCGTCTCCATCGAAA+AGG - chr1.2:44790795-44790814 None:intergenic 45.0%
GACGAGGCATGTTCTTCAAT+AGG - chr1.2:44790510-44790529 None:intergenic 45.0%
TACCTTTATAGCCCATGCGA+AGG - chr1.2:44789769-44789788 None:intergenic 45.0%
TGTCATGTTCCAGATTGTGG+TGG + chr1.2:44791134-44791153 MS.gene45453:CDS 45.0%
TTGTCGCAAAGCTGTCAAGT+TGG + chr1.2:44790003-44790022 MS.gene45453:CDS 45.0%
! TTCCTTCGCATGGGCTATAA+AGG + chr1.2:44789764-44789783 MS.gene45453:CDS 45.0%
ATGACAACGACGAGGCTTCT+TGG - chr1.2:44791120-44791139 None:intergenic 50.0%
! GCAGCTTCAACCCTTCCTTT+TGG - chr1.2:44791098-44791117 None:intergenic 50.0%
! TCTGGAACATGACAACGACG+AGG - chr1.2:44791128-44791147 None:intergenic 50.0%
!! CTCACAGTTTGGTTTGGGTG+TGG + chr1.2:44790133-44790152 MS.gene45453:intron 50.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 44789678 44791339 44789678 ID=MS.gene45453
chr1.2 mRNA 44789678 44791339 44789678 ID=MS.gene45453.t1;Parent=MS.gene45453
chr1.2 exon 44789678 44789785 44789678 ID=MS.gene45453.t1.exon1;Parent=MS.gene45453.t1
chr1.2 CDS 44789678 44789785 44789678 ID=cds.MS.gene45453.t1;Parent=MS.gene45453.t1
chr1.2 exon 44789879 44790079 44789879 ID=MS.gene45453.t1.exon2;Parent=MS.gene45453.t1
chr1.2 CDS 44789879 44790079 44789879 ID=cds.MS.gene45453.t1;Parent=MS.gene45453.t1
chr1.2 exon 44790417 44790659 44790417 ID=MS.gene45453.t1.exon3;Parent=MS.gene45453.t1
chr1.2 CDS 44790417 44790659 44790417 ID=cds.MS.gene45453.t1;Parent=MS.gene45453.t1
chr1.2 exon 44790879 44791231 44790879 ID=MS.gene45453.t1.exon4;Parent=MS.gene45453.t1
chr1.2 CDS 44790879 44791231 44790879 ID=cds.MS.gene45453.t1;Parent=MS.gene45453.t1
chr1.2 exon 44791318 44791339 44791318 ID=MS.gene45453.t1.exon5;Parent=MS.gene45453.t1
chr1.2 CDS 44791318 44791339 44791318 ID=cds.MS.gene45453.t1;Parent=MS.gene45453.t1
Gene Sequence

>MS.gene45453

ATGCCTCTTGGAATATGGGTTGGTGTTGATAATCATGGAAATTCAATTTTTTTTGGTTGCGTTCTATTGCGAAATGAGAAGATTTCTTCCTTCGCATGGGCTATAAAGGTAAATTTCTATTTACTCAAAGTTGCTATCACTGTTAAATTTTTTAATTTGAAACTTTATTAAATTATTTTATATTTATGTTACTATTTTCAGAGTTTCCTAGCTTTTGTGAAAGGAAAATATCCAAAAACAATTCTTACCGATCAAGATCCTTCAATTAAAGAAGAAATTGCAATGGAATTGCCAAACACAAAACATGCATTTTGCATATGGCATATTGTCGCAAAGCTGTCAAGTTGGTTTTCTTTTCCACTAGGTTCGAGATATGATGACTTTAAACATGAGTTTCATAAGGTGTATCATTTAGAATGTGTAGATGATTTTGAACGAGAATGGAAAGTAATGGTCTCACAGTTTGGTTTGGGTGTGGATAAGCATATTGACTTGCTCTTTACCCATCGTAAATTTTGGGCCTTAGCTTATTTGAAGGACTTTTTTTTTGCTGGAATGACAACAACAGGACGCTCAGAATCGATAAATTCATATATAAAAAGATTCTTAGATGCCAAAACAATTCTTAGATGCCAAAACAAGTTTGACAGATTTTGTAAATCAAGTAAGAACTTACTTGATACATTTTTTTTAATCAATTAGCTTATAATTTAGTCATGATTGTTATATTTTTGTGTAGGTTGGTGTTGCTGTCAAAATTAGAAATCAAGCTGGAGAAGAGGCACAAATGCGTCAAAAGTATCATAATCCTCAGATTACAACACATTTTCCTATTGAAGAACATGCCTCGTCTATACTCACACCTTATGCCTTTAAGTTGCTTCAACATGAGATCGAATTATCTGCAAAGTATGCAGAAATTGAAGCTGACAATGGCTCTTATGTTGTGCAACATCATACCAAAGTTGATGGTGGTCGTATAGTAAGTTTGGTAAAAGAAGAAGAGTCAATTCATTGTTCTTGTAAAGAGTTTCAATTTTCAGGAATAATATGTAGACATGCAATTCGAGTGCTAATGTTGAAAAATTATTTTGTTCTTCCATCAAAATATCTTCCTTTTCGATGGAGACGAGAAAGTTCATTGATCCCAAAATCTAGTCATGTAATAAACTGCAATGATGGATCCTCTCCCAAATTTCAGTCTCTAATTCGATGTTTGGAAGTCGAATCATTAAAGACAAGTGAACGAGAACAGGTTGCCACAAGAGAGTTAGAAAATATCATCATAAAGATAAAAGGCATGCCTGAAACTCAAGAACATGTGATTGATTTGGAAGCGGATGTTTCCAATAATGATGATTGTGACGTTGAAAATCCTGTTGTTTCAAAAACAAAAGGTCGGCCAAAAGGATCAAGACCAAAAGGAAGGGTTGAAGCTGCCAAGAAGCCTCGTCGTTGTCATGTTCCAGATTGTGGTGGAACTAATCACGACTCACGAAACTGTCCAAACAAAAAGAGAAAAATGGAAGTGTTGCCTTCTCAATCTCCTAATAAGTAAGTTTTAATTGGTGTGTCTTGTTCTGCTCATGACTAATATATTGAATTGTAATATTAACTGTGTTTTTATGGTGCTTTGATAGGATCATGAAGGGTTGGAAGTAG
Protein sequence

>MS.gene45453.t1

MPLGIWVGVDNHGNSIFFGCVLLRNEKISSFAWAIKSFLAFVKGKYPKTILTDQDPSIKEEIAMELPNTKHAFCIWHIVAKLSSWFSFPLGSRYDDFKHEFHKVGVAVKIRNQAGEEAQMRQKYHNPQITTHFPIEEHASSILTPYAFKLLQHEIELSAKYAEIEADNGSYVVQHHTKVDGGRISLIRCLEVESLKTSEREQVATRELENIIIKIKGMPETQEHVIDLEADVSNNDDCDVENPVVSKTKGRPKGSRPKGRVEAAKKPRRCHVPDCGGTNHDSRNCPNKKRKMEVLPSQSPNKIMKGWK