Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene45464.t1 | RHN61266.1 | 91.7 | 121 | 10 | 0 | 293 | 413 | 326 | 446 | 2.40E-52 | 216.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene45464.t1 | Q9XI99 | 38.2 | 89 | 52 | 1 | 328 | 413 | 58 | 146 | 8.1e-09 | 63.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene45464.t1 | A0A072UL76 | 91.7 | 121 | 10 | 0 | 293 | 413 | 212 | 332 | 1.7e-52 | 216.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051996 | MS.gene45464 | 0.801323 | 9.51E-49 | -1.69E-46 |
| MS.gene052223 | MS.gene45464 | 0.847164 | 1.38E-59 | -1.69E-46 |
| MS.gene056330 | MS.gene45464 | 0.824431 | 8.10E-54 | -1.69E-46 |
| MS.gene056690 | MS.gene45464 | 0.913492 | 5.78E-84 | -1.69E-46 |
| MS.gene056920 | MS.gene45464 | 0.808426 | 3.12E-50 | -1.69E-46 |
| MS.gene060087 | MS.gene45464 | 0.815462 | 9.13E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene45464.t1 | MTR_4g069520 | 70.495 | 444 | 92 | 5 | 5 | 413 | 153 | 592 | 0.0 | 583 |
| MS.gene45464.t1 | MTR_4g069520 | 58.307 | 319 | 66 | 7 | 29 | 292 | 53 | 359 | 4.41e-97 | 306 |
| MS.gene45464.t1 | MTR_4g069520 | 70.495 | 444 | 92 | 5 | 5 | 413 | 139 | 578 | 0.0 | 582 |
| MS.gene45464.t1 | MTR_4g069520 | 58.307 | 319 | 66 | 7 | 29 | 292 | 39 | 345 | 8.01e-97 | 305 |
| MS.gene45464.t1 | MTR_4g069550 | 61.446 | 332 | 66 | 9 | 105 | 413 | 40 | 332 | 2.19e-113 | 340 |
| MS.gene45464.t1 | MTR_4g069550 | 80.667 | 150 | 22 | 2 | 17 | 159 | 55 | 204 | 4.90e-74 | 239 |
| MS.gene45464.t1 | MTR_4g069550 | 81.295 | 139 | 14 | 2 | 1 | 136 | 81 | 210 | 1.11e-67 | 222 |
| MS.gene45464.t1 | MTR_4g069570 | 64.516 | 217 | 49 | 4 | 208 | 413 | 6 | 205 | 2.55e-83 | 258 |
| MS.gene45464.t1 | MTR_4g069570 | 57.292 | 96 | 21 | 2 | 101 | 185 | 2 | 88 | 7.21e-24 | 101 |
| MS.gene45464.t1 | MTR_4g069570 | 63.855 | 83 | 19 | 1 | 161 | 232 | 6 | 88 | 4.99e-23 | 99.4 |
| MS.gene45464.t1 | MTR_4g069570 | 48.246 | 114 | 32 | 3 | 25 | 138 | 2 | 88 | 2.37e-19 | 89.0 |
| MS.gene45464.t1 | MTR_4g069570 | 50.000 | 98 | 31 | 2 | 3 | 100 | 9 | 88 | 5.96e-18 | 84.7 |
| MS.gene45464.t1 | MTR_4g069040 | 68.000 | 100 | 32 | 0 | 314 | 413 | 280 | 379 | 4.30e-42 | 155 |
| MS.gene45464.t1 | MTR_3g116270 | 32.615 | 371 | 191 | 15 | 90 | 413 | 19 | 377 | 4.17e-32 | 127 |
| MS.gene45464.t1 | MTR_4g069540 | 69.136 | 81 | 25 | 0 | 307 | 387 | 13 | 93 | 2.02e-31 | 116 |
| MS.gene45464.t1 | MTR_3g116320 | 44.720 | 161 | 80 | 4 | 256 | 413 | 405 | 559 | 1.01e-30 | 125 |
| MS.gene45464.t1 | MTR_6g086530 | 62.667 | 75 | 27 | 1 | 340 | 413 | 1 | 75 | 7.89e-23 | 95.9 |
| MS.gene45464.t1 | MTR_4g069680 | 46.067 | 89 | 45 | 2 | 328 | 413 | 70 | 158 | 5.91e-18 | 84.3 |
| MS.gene45464.t1 | MTR_3g107830 | 47.191 | 89 | 44 | 2 | 328 | 413 | 39 | 127 | 3.27e-16 | 78.6 |
| MS.gene45464.t1 | MTR_3g116410 | 43.820 | 89 | 47 | 2 | 328 | 413 | 59 | 147 | 6.13e-12 | 66.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene45464.t1 | AT1G49320 | 38.202 | 89 | 52 | 1 | 328 | 413 | 58 | 146 | 8.77e-12 | 65.9 |
Find 148 sgRNAs with CRISPR-Local
Find 144 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCCATTGGATTCTGATTGTT+TGG | 0.238831 | 4.1:-31951586 | None:intergenic |
| TTGTACCCGGTAGTGTAATC+TGG | 0.274373 | 4.1:-31951507 | None:intergenic |
| ATGTACCCGTTAAGGTAATT+TGG | 0.281112 | 4.1:-31951925 | None:intergenic |
| TCCATTGGATTCTGATTGTT+TGG | 0.283923 | 4.1:-31951727 | None:intergenic |
| TCCATTGGATTCTTGTTGTT+TGG | 0.293372 | 4.1:-31951781 | None:intergenic |
| GGTAGTGTAATCTGGGTTCT+TGG | 0.317068 | 4.1:-31951499 | None:intergenic |
| GGTAGTGTAACCTGGGTTCT+TGG | 0.319597 | 4.1:-31951640 | None:intergenic |
| TGTAGTGTAATCCGGGTTCT+TGG | 0.321752 | 4.1:-31951016 | None:intergenic |
| TGTAGTGTAATCCGGGTTCT+TGG | 0.321752 | 4.1:-31951130 | None:intergenic |
| TGTAGTGTAATCCGGGTTCT+TGG | 0.321752 | 4.1:-31951244 | None:intergenic |
| TGTAGTGTAATCCGGGTTCT+TGG | 0.321752 | 4.1:-31951358 | None:intergenic |
| TTAGCTTGAGGAGAATCTTC+AGG | 0.325821 | 4.1:-31952201 | None:intergenic |
| CCCATTGGAATCCAGTTGTT+TGG | 0.334877 | 4.1:-31951445 | None:intergenic |
| GGGCCTTACATTACTCAATA+TGG | 0.344598 | 4.1:+31951465 | MS.gene45464:CDS |
| GTGGTCCAAGTTAGGAGATT+GGG | 0.344871 | 4.1:-31952007 | None:intergenic |
| GGACCTTACATAACTCAATA+TGG | 0.345216 | 4.1:+31951747 | MS.gene45464:CDS |
| GGCAGTGTTGTAAGGTTCAT+TGG | 0.362108 | 4.1:-31951688 | None:intergenic |
| AGATCGTTTGAGGTGGTAGA+TGG | 0.363028 | 4.1:-31951976 | None:intergenic |
| TGTGAATCGGAAACCATTAC+AGG | 0.374526 | 4.1:+31952249 | MS.gene45464:CDS |
| TTTCATTGGATTCATGGGTA+TGG | 0.379290 | 4.1:-31951278 | None:intergenic |
| TTGTACCCTGTAGTGTAATC+CGG | 0.392483 | 4.1:-31951024 | None:intergenic |
| TTGTACCCTGTAGTGTAATC+CGG | 0.392483 | 4.1:-31951138 | None:intergenic |
| TTGTACCCTGTAGTGTAATC+CGG | 0.392483 | 4.1:-31951252 | None:intergenic |
| TTGTACCCTGTAGTGTAATC+CGG | 0.392483 | 4.1:-31951366 | None:intergenic |
| TTGTACCCGGTAGTGTAACC+TGG | 0.394466 | 4.1:-31951648 | None:intergenic |
| TTTCATTAGATTCATGGGTA+TGG | 0.395484 | 4.1:-31951050 | None:intergenic |
| CAGCAGATATGTACCCGTTA+AGG | 0.400401 | 4.1:-31951933 | None:intergenic |
| TTCATTAGATTCATGGGAAT+GGG | 0.402557 | 4.1:-31951163 | None:intergenic |
| AGCAACCAAATTACCTTAAC+GGG | 0.404392 | 4.1:+31951920 | MS.gene45464:CDS |
| GGCAGTGTTGTAAGGTTCAA+TGG | 0.404622 | 4.1:-31951547 | None:intergenic |
| TTCATTGGATTCATGGGTAT+GGG | 0.405636 | 4.1:-31951277 | None:intergenic |
| TGTAAGGTCCACTGGATTCA+TGG | 0.411259 | 4.1:-31951398 | None:intergenic |
| TTCATTAGATTCATGGGTAT+GGG | 0.412584 | 4.1:-31951049 | None:intergenic |
| TGTAAGGTTCAATGGATTCA+TGG | 0.414653 | 4.1:-31951539 | None:intergenic |
| GTTCATTGGATGCATGGGTA+TGG | 0.417794 | 4.1:-31951674 | None:intergenic |
| CTCTCCAAGTGAATTTATCA+TGG | 0.423407 | 4.1:-31952226 | None:intergenic |
| GGGGCCTTCTAGATCGTTTG+AGG | 0.424265 | 4.1:-31951986 | None:intergenic |
| GTTAAGGTAATTTGGTTGCT+TGG | 0.424962 | 4.1:-31951917 | None:intergenic |
| GTCCACTGGATTCATGGGTA+TGG | 0.427423 | 4.1:-31951392 | None:intergenic |
| GCTTCTGTGTGGTCCAAGTT+AGG | 0.430125 | 4.1:-31952015 | None:intergenic |
| TTGAGTTATGTAAGGTCCAT+TGG | 0.433211 | 4.1:-31951742 | None:intergenic |
| AGTCTTTGTCTCCCCTGTAA+TGG | 0.434357 | 4.1:-31952262 | None:intergenic |
| GGACCACACAGAAGCTTTCA+AGG | 0.436009 | 4.1:+31952023 | MS.gene45464:CDS |
| TTACGATATTGAGTTATGTA+AGG | 0.441136 | 4.1:-31951609 | None:intergenic |
| GTTCAATGGATTCATGGGTA+TGG | 0.445281 | 4.1:-31951533 | None:intergenic |
| AAGACATGAAGCCAAACAAC+TGG | 0.447852 | 4.1:+31951434 | MS.gene45464:CDS |
| TTACCATATTGAGTTATGTA+AGG | 0.449605 | 4.1:-31951750 | None:intergenic |
| AAGCAACCAAATTACCTTAA+CGG | 0.454443 | 4.1:+31951919 | MS.gene45464:CDS |
| TGTGGTCCAAGTTAGGAGAT+TGG | 0.455176 | 4.1:-31952008 | None:intergenic |
| TTACCATAGGCAGTGTTGTA+AGG | 0.456880 | 4.1:-31951696 | None:intergenic |
| GAACCTTACAACACTGCCTA+TGG | 0.460056 | 4.1:+31951693 | MS.gene45464:CDS |
| TTTCATTAGATTCATGGGAA+TGG | 0.461704 | 4.1:-31951164 | None:intergenic |
| GGAAGTTGTGTAAGGTCCAT+TGG | 0.462228 | 4.1:-31951796 | None:intergenic |
| TTGAGTTATGTAAGGCCCAT+TGG | 0.465944 | 4.1:-31951601 | None:intergenic |
| TTACCATATTGAGTAATGTA+AGG | 0.470153 | 4.1:-31951468 | None:intergenic |
| TTAGCATAGGCAGTGTTGTA+AGG | 0.475110 | 4.1:-31951555 | None:intergenic |
| TTACCATAGGAAGTTGTGTA+AGG | 0.475470 | 4.1:-31951804 | None:intergenic |
| GATCGTTTGAGGTGGTAGAT+GGG | 0.479040 | 4.1:-31951975 | None:intergenic |
| TTCATTGGATGCATGGGTAT+GGG | 0.479659 | 4.1:-31951673 | None:intergenic |
| TAGCTTGAGGAGAATCTTCA+GGG | 0.480432 | 4.1:-31952200 | None:intergenic |
| CTGTATCCGCCAGTCATGTA+AGG | 0.482850 | 4.1:-31951084 | None:intergenic |
| CTGTACCCGCCAGTCATGTA+AGG | 0.482850 | 4.1:-31951198 | None:intergenic |
| CTGTACCCGCCAGTCATGTA+AGG | 0.482850 | 4.1:-31951312 | None:intergenic |
| GGCAGTTATGTAAGGTCCAC+TGG | 0.487353 | 4.1:-31951406 | None:intergenic |
| CTTCCGTAGGCAGTTATGTA+AGG | 0.488428 | 4.1:-31951414 | None:intergenic |
| GGACCTTACACAACTTCCTA+TGG | 0.490690 | 4.1:+31951801 | MS.gene45464:CDS |
| CTAAGTGAGCCTTACATGAC+TGG | 0.499302 | 4.1:+31951189 | MS.gene45464:CDS |
| ACCACCTCAAACGATCTAGA+AGG | 0.500418 | 4.1:+31951982 | MS.gene45464:CDS |
| TTGAGTAATGTAAGGCCCAT+TGG | 0.501940 | 4.1:-31951460 | None:intergenic |
| TTTATCATGGATTTAGCTTG+AGG | 0.502246 | 4.1:-31952213 | None:intergenic |
| CCACTTGGACGGGAAATTGA+AGG | 0.503790 | 4.1:-31952092 | None:intergenic |
| CTAAGCGAGCCTTACATGAC+TGG | 0.509211 | 4.1:+31950961 | None:intergenic |
| CTAAGCGAGCCTTACATGAC+TGG | 0.509211 | 4.1:+31951075 | MS.gene45464:CDS |
| CTAAGCGAGCCTTACATGAC+TGG | 0.509211 | 4.1:+31951303 | MS.gene45464:CDS |
| ATCGTTTGAGGTGGTAGATG+GGG | 0.509770 | 4.1:-31951974 | None:intergenic |
| AAGAACCCGGATTACACTAC+AGG | 0.510637 | 4.1:+31951018 | MS.gene45464:CDS |
| AAGAACCCGGATTACACTAC+AGG | 0.510637 | 4.1:+31951132 | MS.gene45464:CDS |
| AAGAACCCGGATTACACTAC+AGG | 0.510637 | 4.1:+31951246 | MS.gene45464:CDS |
| AAGAACCCGGATTACACTAC+AGG | 0.510637 | 4.1:+31951360 | MS.gene45464:CDS |
| TTCACTTGGAGAGTGTGAAT+CGG | 0.519976 | 4.1:+31952236 | MS.gene45464:CDS |
| TGGTCCAAGTTAGGAGATTG+GGG | 0.526114 | 4.1:-31952006 | None:intergenic |
| TCCACTGGATTCATGGGTAT+GGG | 0.527581 | 4.1:-31951391 | None:intergenic |
| TTCAATGGATTCATGGGTAT+GGG | 0.529636 | 4.1:-31951532 | None:intergenic |
| TCGTTTGAGGTGGTAGATGG+GGG | 0.531816 | 4.1:-31951973 | None:intergenic |
| TGTACCCGGTAGTGTAATCT+GGG | 0.532124 | 4.1:-31951506 | None:intergenic |
| GTAAGGTTCAATGGATTCAT+GGG | 0.532589 | 4.1:-31951538 | None:intergenic |
| TTTGGCTTCATGTTTACCAT+AGG | 0.532676 | 4.1:-31951709 | None:intergenic |
| CTTAACGGGTACATATCTGC+TGG | 0.538096 | 4.1:+31951934 | MS.gene45464:CDS |
| TCTGCTGGAAATGACCCAAA+AGG | 0.545928 | 4.1:+31951949 | MS.gene45464:CDS |
| CCAAACAACTGGATTCCAAT+GGG | 0.546622 | 4.1:+31951445 | MS.gene45464:CDS |
| GTAAGGTCCACTGGATTCAT+GGG | 0.548211 | 4.1:-31951397 | None:intergenic |
| TTTGGCTTCATGTCTTCCGT+AGG | 0.548233 | 4.1:-31951427 | None:intergenic |
| CCAAACAATCAGAATCCAAT+GGG | 0.549989 | 4.1:+31951586 | MS.gene45464:CDS |
| TGTACCCTGTAGTGTAATCC+GGG | 0.551924 | 4.1:-31951023 | None:intergenic |
| TGTACCCTGTAGTGTAATCC+GGG | 0.551924 | 4.1:-31951137 | None:intergenic |
| TGTACCCTGTAGTGTAATCC+GGG | 0.551924 | 4.1:-31951251 | None:intergenic |
| TGTACCCTGTAGTGTAATCC+GGG | 0.551924 | 4.1:-31951365 | None:intergenic |
| AAGAACCCAGGTTACACTAC+CGG | 0.552435 | 4.1:+31951642 | MS.gene45464:CDS |
| AAGAACCCAGATTACACTAC+CGG | 0.556042 | 4.1:+31951501 | MS.gene45464:CDS |
| GGCGGGTACAGACTTGCATA+TGG | 0.556538 | 4.1:+31950982 | MS.gene45464:CDS |
| GGCGGGTACAGACTTGCATA+TGG | 0.556538 | 4.1:+31951324 | MS.gene45464:CDS |
| AAAGGAGAAACCACTTGGAC+GGG | 0.559421 | 4.1:-31952102 | None:intergenic |
| TGTAAGGTTCATTGGATGCA+TGG | 0.559989 | 4.1:-31951680 | None:intergenic |
| TTGTGACATTGCAAGAGGAA+TGG | 0.560283 | 4.1:-31952148 | None:intergenic |
| GCATATGGACTCTAGAGAGT+TGG | 0.564218 | 4.1:-31952289 | None:intergenic |
| TTTGGCTTCATGTTTAGCAT+AGG | 0.568199 | 4.1:-31951568 | None:intergenic |
| GCCAAACAACTGGATTCCAA+TGG | 0.569111 | 4.1:+31951444 | MS.gene45464:CDS |
| TGTACCCGGTAGTGTAACCT+GGG | 0.570132 | 4.1:-31951647 | None:intergenic |
| ATGGGTATGGGTCTTGTACC+CGG | 0.573227 | 4.1:-31951520 | None:intergenic |
| GCCAAACAATCAGAATCCAA+TGG | 0.580573 | 4.1:+31951585 | MS.gene45464:CDS |
| GTAAGGTTCATTGGATGCAT+GGG | 0.583311 | 4.1:-31951679 | None:intergenic |
| CCTTCAATTTCCCGTCCAAG+TGG | 0.588140 | 4.1:+31952092 | MS.gene45464:CDS |
| AGAACCCAGGTTACACTACC+GGG | 0.588345 | 4.1:+31951643 | MS.gene45464:CDS |
| TAAACATGAAGCCAAGAACC+CGG | 0.590476 | 4.1:+31951005 | MS.gene45464:CDS |
| TAAACATGAAGCCAAGAACC+CGG | 0.590476 | 4.1:+31951347 | MS.gene45464:CDS |
| GGACCTTACATAACTGCCTA+CGG | 0.593355 | 4.1:+31951411 | MS.gene45464:CDS |
| CACACAGAAGCTTTCAAGGT+AGG | 0.597254 | 4.1:+31952027 | MS.gene45464:CDS |
| GAAAGGAGAAACCACTTGGA+CGG | 0.598116 | 4.1:-31952103 | None:intergenic |
| CACTTGGACGGGAAATTGAA+GGG | 0.598885 | 4.1:-31952091 | None:intergenic |
| AGAGGAAAGGAGAAACCACT+TGG | 0.600974 | 4.1:-31952107 | None:intergenic |
| TAAACATGAAGCCAAGAACC+CGG | 0.602710 | 4.1:+31951119 | MS.gene45464:CDS |
| TAAACATGAAGCCAAGAACC+CGG | 0.602710 | 4.1:+31951233 | MS.gene45464:CDS |
| GTGAGCCTTACATGACTGGC+GGG | 0.603005 | 4.1:+31951193 | MS.gene45464:CDS |
| AAATCCATGATAAATTCACT+TGG | 0.603290 | 4.1:+31952222 | MS.gene45464:CDS |
| GGGAGTTGTGACATTGCAAG+AGG | 0.609821 | 4.1:-31952153 | None:intergenic |
| GCCAAACAATCAGAATCCAA+TGG | 0.613339 | 4.1:+31951726 | MS.gene45464:CDS |
| GCCTTCTAGATCGTTTGAGG+TGG | 0.619752 | 4.1:-31951983 | None:intergenic |
| GCCAAACAACAAGAATCCAA+TGG | 0.625758 | 4.1:+31951780 | MS.gene45464:CDS |
| GCGAGCCTTACATGACTGGC+GGG | 0.626816 | 4.1:+31950965 | None:intergenic |
| GCGAGCCTTACATGACTGGC+GGG | 0.626816 | 4.1:+31951307 | MS.gene45464:CDS |
| CGTTTGAGGTGGTAGATGGG+GGG | 0.627717 | 4.1:-31951972 | None:intergenic |
| GTGAATCGGAAACCATTACA+GGG | 0.628683 | 4.1:+31952250 | MS.gene45464:CDS |
| GGCGGATACAGACTTGCATA+CGG | 0.637048 | 4.1:+31951096 | MS.gene45464:CDS |
| AGCGAGCCTTACATGACTGG+CGG | 0.648735 | 4.1:+31950964 | None:intergenic |
| AGCGAGCCTTACATGACTGG+CGG | 0.648735 | 4.1:+31951306 | MS.gene45464:CDS |
| ACCCATACCCATGAATCCAG+TGG | 0.653332 | 4.1:+31951390 | MS.gene45464:CDS |
| GGCGGGTACAGACTTGCATA+CGG | 0.658419 | 4.1:+31951210 | MS.gene45464:CDS |
| AGAACCCAGATTACACTACC+GGG | 0.659254 | 4.1:+31951502 | MS.gene45464:CDS |
| AGTGAGCCTTACATGACTGG+CGG | 0.663436 | 4.1:+31951192 | MS.gene45464:CDS |
| AGCGAGCCTTACATGACTGG+CGG | 0.688215 | 4.1:+31951078 | MS.gene45464:CDS |
| AGAACCCGGATTACACTACA+GGG | 0.688478 | 4.1:+31951019 | MS.gene45464:CDS |
| AGAACCCGGATTACACTACA+GGG | 0.688478 | 4.1:+31951133 | MS.gene45464:CDS |
| AGAACCCGGATTACACTACA+GGG | 0.688478 | 4.1:+31951247 | MS.gene45464:CDS |
| AGAACCCGGATTACACTACA+GGG | 0.688478 | 4.1:+31951361 | MS.gene45464:CDS |
| GGTCCAAGTTAGGAGATTGG+GGG | 0.690347 | 4.1:-31952005 | None:intergenic |
| AAACATGAAACCAAGAACCC+AGG | 0.692067 | 4.1:+31951630 | MS.gene45464:CDS |
| CTACCTTGAAAGCTTCTGTG+TGG | 0.713851 | 4.1:-31952026 | None:intergenic |
| TGAATCGGAAACCATTACAG+GGG | 0.744089 | 4.1:+31952251 | MS.gene45464:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAATCCATGATAAATTCACT+TGG | + | chr4.1:31952222-31952241 | MS.gene45464:CDS | 25.0% |
| ! | TTACCATATTGAGTAATGTA+AGG | - | chr4.1:31951471-31951490 | None:intergenic | 25.0% |
| ! | TTACCATATTGAGTTATGTA+AGG | - | chr4.1:31951753-31951772 | None:intergenic | 25.0% |
| ! | TTACGATATTGAGTTATGTA+AGG | - | chr4.1:31951612-31951631 | None:intergenic | 25.0% |
| !! | TAGCTTTTCATTAGATTCAT+GGG | - | chr4.1:31951058-31951077 | None:intergenic | 25.0% |
| !!! | TTAGCTTTTCATTAGATTCA+TGG | - | chr4.1:31951059-31951078 | None:intergenic | 25.0% |
| AAGCAACAAAGTTACAATAC+CGG | + | chr4.1:31951837-31951856 | MS.gene45464:intron | 30.0% | |
| AAGCAACCAAATTACCTTAA+CGG | + | chr4.1:31951919-31951938 | MS.gene45464:CDS | 30.0% | |
| AGAAGAGTTGAAAAACACTT+GGG | - | chr4.1:31952176-31952195 | None:intergenic | 30.0% | |
| GTACAATTCATTAGATTCCT+GGG | - | chr4.1:31951872-31951891 | None:intergenic | 30.0% | |
| TTCATTAGATTCATGGGAAT+GGG | - | chr4.1:31951166-31951185 | None:intergenic | 30.0% | |
| TTCATTAGATTCATGGGTAT+GGG | - | chr4.1:31951052-31951071 | None:intergenic | 30.0% | |
| TTTCATTAGATTCATGGGAA+TGG | - | chr4.1:31951167-31951186 | None:intergenic | 30.0% | |
| TTTCATTAGATTCATGGGTA+TGG | - | chr4.1:31951053-31951072 | None:intergenic | 30.0% | |
| ! | TAGCTTTTCATTGGATTCAT+GGG | - | chr4.1:31951286-31951305 | None:intergenic | 30.0% |
| !! | CAAGGTAGGATTTTTCAATT+TGG | + | chr4.1:31952041-31952060 | MS.gene45464:CDS | 30.0% |
| !! | TTAGCTTTTCATTGGATTCA+TGG | - | chr4.1:31951287-31951306 | None:intergenic | 30.0% |
| !! | TTTATCATGGATTTAGCTTG+AGG | - | chr4.1:31952216-31952235 | None:intergenic | 30.0% |
| AATACCGGATATAAATACCC+AGG | + | chr4.1:31951852-31951871 | MS.gene45464:intron | 35.0% | |
| AGCAACCAAATTACCTTAAC+GGG | + | chr4.1:31951920-31951939 | MS.gene45464:CDS | 35.0% | |
| ATGTACCCGTTAAGGTAATT+TGG | - | chr4.1:31951928-31951947 | None:intergenic | 35.0% | |
| CCAAACAATCAGAATCCAAT+GGG | + | chr4.1:31951586-31951605 | MS.gene45464:CDS | 35.0% | |
| CGTACAATTCATTAGATTCC+TGG | - | chr4.1:31951873-31951892 | None:intergenic | 35.0% | |
| CTCTCCAAGTGAATTTATCA+TGG | - | chr4.1:31952229-31952248 | None:intergenic | 35.0% | |
| GAATTGTACGTATCTGCATA+TGG | + | chr4.1:31951883-31951902 | MS.gene45464:intron | 35.0% | |
| GAGAAGAGTTGAAAAACACT+TGG | - | chr4.1:31952177-31952196 | None:intergenic | 35.0% | |
| GATTCCTGGGTATTTATATC+CGG | - | chr4.1:31951859-31951878 | None:intergenic | 35.0% | |
| GGACCTTACATAACTCAATA+TGG | + | chr4.1:31951747-31951766 | MS.gene45464:CDS | 35.0% | |
| GTTAAGGTAATTTGGTTGCT+TGG | - | chr4.1:31951920-31951939 | None:intergenic | 35.0% | |
| TCCATTGGATTCTGATTGTT+TGG | - | chr4.1:31951730-31951749 | None:intergenic | 35.0% | |
| TTACCATAGGAAGTTGTGTA+AGG | - | chr4.1:31951807-31951826 | None:intergenic | 35.0% | |
| TTCATTGGATTCATGGGTAT+GGG | - | chr4.1:31951280-31951299 | None:intergenic | 35.0% | |
| TTGAGTTATGTAAGGTCCAT+TGG | - | chr4.1:31951745-31951764 | None:intergenic | 35.0% | |
| TTTCATTGGATTCATGGGTA+TGG | - | chr4.1:31951281-31951300 | None:intergenic | 35.0% | |
| ! | CTTTGCTTCTTGTTTACCAT+AGG | - | chr4.1:31951820-31951839 | None:intergenic | 35.0% |
| ! | GTAAGGTTCAATGGATTCAT+GGG | - | chr4.1:31951541-31951560 | None:intergenic | 35.0% |
| ! | TCCTTTCCTCTCTAAAAAAG+AGG | + | chr4.1:31952119-31952138 | MS.gene45464:CDS | 35.0% |
| ! | TGTAAGGTTCAATGGATTCA+TGG | - | chr4.1:31951542-31951561 | None:intergenic | 35.0% |
| ! | TTCAATGGATTCATGGGTAT+GGG | - | chr4.1:31951535-31951554 | None:intergenic | 35.0% |
| ! | TTTGGCTTCATGTTTACCAT+AGG | - | chr4.1:31951712-31951731 | None:intergenic | 35.0% |
| ! | TTTGGCTTCATGTTTAGCAT+AGG | - | chr4.1:31951571-31951590 | None:intergenic | 35.0% |
| !! | TCCATTGGATTCTTGTTGTT+TGG | - | chr4.1:31951784-31951803 | None:intergenic | 35.0% |
| AAACATGAAACCAAGAACCC+AGG | + | chr4.1:31951489-31951508 | MS.gene45464:CDS | 40.0% | |
| AAGAACCCAGATTACACTAC+CGG | + | chr4.1:31951501-31951520 | MS.gene45464:CDS | 40.0% | |
| AAGACATGAAGCCAAACAAC+TGG | + | chr4.1:31951434-31951453 | MS.gene45464:CDS | 40.0% | |
| AATTTGGTTGCTTGGCTTCA+TGG | - | chr4.1:31951912-31951931 | None:intergenic | 40.0% | |
| CCAAACAACTGGATTCCAAT+GGG | + | chr4.1:31951445-31951464 | MS.gene45464:CDS | 40.0% | |
| CCCATTGGATTCTGATTGTT+TGG | - | chr4.1:31951589-31951608 | None:intergenic | 40.0% | |
| GCCAAACAACAAGAATCCAA+TGG | + | chr4.1:31951780-31951799 | MS.gene45464:CDS | 40.0% | |
| GCCAAACAATCAGAATCCAA+TGG | + | chr4.1:31951585-31951604 | MS.gene45464:CDS | 40.0% | |
| GGGCCTTACATTACTCAATA+TGG | + | chr4.1:31951465-31951484 | MS.gene45464:CDS | 40.0% | |
| GTAAGGTTCATTGGATGCAT+GGG | - | chr4.1:31951682-31951701 | None:intergenic | 40.0% | |
| GTGAATCGGAAACCATTACA+GGG | + | chr4.1:31952250-31952269 | MS.gene45464:CDS | 40.0% | |
| TAAACATGAAGCCAAGAACC+CGG | + | chr4.1:31951005-31951024 | MS.gene45464:CDS | 40.0% | |
| TAGCTTGAGGAGAATCTTCA+GGG | - | chr4.1:31952203-31952222 | None:intergenic | 40.0% | |
| TGAATCGGAAACCATTACAG+GGG | + | chr4.1:31952251-31952270 | MS.gene45464:CDS | 40.0% | |
| TGTAAGGTTCATTGGATGCA+TGG | - | chr4.1:31951683-31951702 | None:intergenic | 40.0% | |
| TGTGAATCGGAAACCATTAC+AGG | + | chr4.1:31952249-31952268 | MS.gene45464:CDS | 40.0% | |
| TTACCATAGGCAGTGTTGTA+AGG | - | chr4.1:31951699-31951718 | None:intergenic | 40.0% | |
| TTAGCATAGGCAGTGTTGTA+AGG | - | chr4.1:31951558-31951577 | None:intergenic | 40.0% | |
| TTCATTGGATGCATGGGTAT+GGG | - | chr4.1:31951676-31951695 | None:intergenic | 40.0% | |
| TTGAGTAATGTAAGGCCCAT+TGG | - | chr4.1:31951463-31951482 | None:intergenic | 40.0% | |
| TTGAGTTATGTAAGGCCCAT+TGG | - | chr4.1:31951604-31951623 | None:intergenic | 40.0% | |
| TTGTACCCTGTAGTGTAATC+CGG | - | chr4.1:31951027-31951046 | None:intergenic | 40.0% | |
| TTGTGACATTGCAAGAGGAA+TGG | - | chr4.1:31952151-31952170 | None:intergenic | 40.0% | |
| ! | GTTCAATGGATTCATGGGTA+TGG | - | chr4.1:31951536-31951555 | None:intergenic | 40.0% |
| ! | TTAGCTTGAGGAGAATCTTC+AGG | - | chr4.1:31952204-31952223 | None:intergenic | 40.0% |
| ! | TTCACTTGGAGAGTGTGAAT+CGG | + | chr4.1:31952236-31952255 | MS.gene45464:CDS | 40.0% |
| !!! | AGTCAGCCTCTTTTTTAGAG+AGG | - | chr4.1:31952128-31952147 | None:intergenic | 40.0% |
| !!! | GCCTCTTTTTTAGAGAGGAA+AGG | - | chr4.1:31952123-31952142 | None:intergenic | 40.0% |
| AAAGGAGAAACCACTTGGAC+GGG | - | chr4.1:31952105-31952124 | None:intergenic | 45.0% | |
| AAGAACCCAGGTTACACTAC+CGG | + | chr4.1:31951642-31951661 | MS.gene45464:CDS | 45.0% | |
| AAGAACCCGGATTACACTAC+AGG | + | chr4.1:31951018-31951037 | MS.gene45464:CDS | 45.0% | |
| ACCACCTCAAACGATCTAGA+AGG | + | chr4.1:31951982-31952001 | MS.gene45464:CDS | 45.0% | |
| AGAACCCAGATTACACTACC+GGG | + | chr4.1:31951502-31951521 | MS.gene45464:CDS | 45.0% | |
| AGAACCCGGATTACACTACA+GGG | + | chr4.1:31951019-31951038 | MS.gene45464:CDS | 45.0% | |
| AGAGGAAAGGAGAAACCACT+TGG | - | chr4.1:31952110-31952129 | None:intergenic | 45.0% | |
| AGTCTTTGTCTCCCCTGTAA+TGG | - | chr4.1:31952265-31952284 | None:intergenic | 45.0% | |
| CACACAGAAGCTTTCAAGGT+AGG | + | chr4.1:31952027-31952046 | MS.gene45464:CDS | 45.0% | |
| CACTTGGACGGGAAATTGAA+GGG | - | chr4.1:31952094-31952113 | None:intergenic | 45.0% | |
| CAGCAGATATGTACCCGTTA+AGG | - | chr4.1:31951936-31951955 | None:intergenic | 45.0% | |
| CCCATTGGAATCCAGTTGTT+TGG | - | chr4.1:31951448-31951467 | None:intergenic | 45.0% | |
| CTAAGTGAGCCTTACATGAC+TGG | + | chr4.1:31951189-31951208 | MS.gene45464:CDS | 45.0% | |
| CTACCTTGAAAGCTTCTGTG+TGG | - | chr4.1:31952029-31952048 | None:intergenic | 45.0% | |
| CTTCCGTAGGCAGTTATGTA+AGG | - | chr4.1:31951417-31951436 | None:intergenic | 45.0% | |
| GAAAGGAGAAACCACTTGGA+CGG | - | chr4.1:31952106-31952125 | None:intergenic | 45.0% | |
| GAACCTTACAACACTGCCTA+TGG | + | chr4.1:31951552-31951571 | MS.gene45464:CDS | 45.0% | |
| GCCAAACAACTGGATTCCAA+TGG | + | chr4.1:31951444-31951463 | MS.gene45464:CDS | 45.0% | |
| GGAAGTTGTGTAAGGTCCAT+TGG | - | chr4.1:31951799-31951818 | None:intergenic | 45.0% | |
| GGACCTTACACAACTTCCTA+TGG | + | chr4.1:31951801-31951820 | MS.gene45464:CDS | 45.0% | |
| GGACCTTACATAACTGCCTA+CGG | + | chr4.1:31951411-31951430 | MS.gene45464:CDS | 45.0% | |
| GGCAGTGTTGTAAGGTTCAA+TGG | - | chr4.1:31951550-31951569 | None:intergenic | 45.0% | |
| GGCAGTGTTGTAAGGTTCAT+TGG | - | chr4.1:31951691-31951710 | None:intergenic | 45.0% | |
| GGTAGTGTAATCTGGGTTCT+TGG | - | chr4.1:31951502-31951521 | None:intergenic | 45.0% | |
| GTAAGGTCCACTGGATTCAT+GGG | - | chr4.1:31951400-31951419 | None:intergenic | 45.0% | |
| GTGGTCCAAGTTAGGAGATT+GGG | - | chr4.1:31952010-31952029 | None:intergenic | 45.0% | |
| GTTCATTGGATGCATGGGTA+TGG | - | chr4.1:31951677-31951696 | None:intergenic | 45.0% | |
| TCCACTGGATTCATGGGTAT+GGG | - | chr4.1:31951394-31951413 | None:intergenic | 45.0% | |
| TCTGCTGGAAATGACCCAAA+AGG | + | chr4.1:31951949-31951968 | MS.gene45464:CDS | 45.0% | |
| TGGTCCAAGTTAGGAGATTG+GGG | - | chr4.1:31952009-31952028 | None:intergenic | 45.0% | |
| TGTAAGGTCCACTGGATTCA+TGG | - | chr4.1:31951401-31951420 | None:intergenic | 45.0% | |
| TGTACCCGGTAGTGTAATCT+GGG | - | chr4.1:31951509-31951528 | None:intergenic | 45.0% | |
| TGTACCCTGTAGTGTAATCC+GGG | - | chr4.1:31951026-31951045 | None:intergenic | 45.0% | |
| TGTAGTGTAATCCGGGTTCT+TGG | - | chr4.1:31951019-31951038 | None:intergenic | 45.0% | |
| TGTGGTCCAAGTTAGGAGAT+TGG | - | chr4.1:31952011-31952030 | None:intergenic | 45.0% | |
| TTGTACCCGGTAGTGTAATC+TGG | - | chr4.1:31951510-31951529 | None:intergenic | 45.0% | |
| ! | AGATCGTTTGAGGTGGTAGA+TGG | - | chr4.1:31951979-31951998 | None:intergenic | 45.0% |
| ! | ATCGTTTGAGGTGGTAGATG+GGG | - | chr4.1:31951977-31951996 | None:intergenic | 45.0% |
| ! | CTTAACGGGTACATATCTGC+TGG | + | chr4.1:31951934-31951953 | MS.gene45464:CDS | 45.0% |
| ! | GATCGTTTGAGGTGGTAGAT+GGG | - | chr4.1:31951978-31951997 | None:intergenic | 45.0% |
| ! | TTTGGCTTCATGTCTTCCGT+AGG | - | chr4.1:31951430-31951449 | None:intergenic | 45.0% |
| !! | AGGCTCGCTTAGCTTTTCAT+TGG | - | chr4.1:31951067-31951086 | None:intergenic | 45.0% |
| !! | ATGGGTATGGGTTTTGTACC+CGG | - | chr4.1:31951664-31951683 | None:intergenic | 45.0% |
| ACCCATACCCATGAATCCAG+TGG | + | chr4.1:31951390-31951409 | MS.gene45464:CDS | 50.0% | |
| AGAACCCAGGTTACACTACC+GGG | + | chr4.1:31951643-31951662 | MS.gene45464:CDS | 50.0% | |
| AGTGAGCCTTACATGACTGG+CGG | + | chr4.1:31951192-31951211 | MS.gene45464:CDS | 50.0% | |
| CCACTTGGACGGGAAATTGA+AGG | - | chr4.1:31952095-31952114 | None:intergenic | 50.0% | |
| CCTTCAATTTCCCGTCCAAG+TGG | + | chr4.1:31952092-31952111 | MS.gene45464:CDS | 50.0% | |
| CTAAGCGAGCCTTACATGAC+TGG | + | chr4.1:31951075-31951094 | MS.gene45464:CDS | 50.0% | |
| CTGTATCCGCCAGTCATGTA+AGG | - | chr4.1:31951087-31951106 | None:intergenic | 50.0% | |
| GCCTTCTAGATCGTTTGAGG+TGG | - | chr4.1:31951986-31952005 | None:intergenic | 50.0% | |
| GCTTCTGTGTGGTCCAAGTT+AGG | - | chr4.1:31952018-31952037 | None:intergenic | 50.0% | |
| GGACCACACAGAAGCTTTCA+AGG | + | chr4.1:31952023-31952042 | MS.gene45464:CDS | 50.0% | |
| GGCAGTTATGTAAGGTCCAC+TGG | - | chr4.1:31951409-31951428 | None:intergenic | 50.0% | |
| GGCGGATACAGACTTGCATA+CGG | + | chr4.1:31951096-31951115 | MS.gene45464:CDS | 50.0% | |
| GGGAGTTGTGACATTGCAAG+AGG | - | chr4.1:31952156-31952175 | None:intergenic | 50.0% | |
| GGTAGTGTAACCTGGGTTCT+TGG | - | chr4.1:31951643-31951662 | None:intergenic | 50.0% | |
| GGTCCAAGTTAGGAGATTGG+GGG | - | chr4.1:31952008-31952027 | None:intergenic | 50.0% | |
| GTCCACTGGATTCATGGGTA+TGG | - | chr4.1:31951395-31951414 | None:intergenic | 50.0% | |
| TGTACCCGGTAGTGTAACCT+GGG | - | chr4.1:31951650-31951669 | None:intergenic | 50.0% | |
| TTGTACCCGGTAGTGTAACC+TGG | - | chr4.1:31951651-31951670 | None:intergenic | 50.0% | |
| ! | ATGGGTATGGGTCTTGTACC+CGG | - | chr4.1:31951040-31951059 | None:intergenic | 50.0% |
| ! | TCGTTTGAGGTGGTAGATGG+GGG | - | chr4.1:31951976-31951995 | None:intergenic | 50.0% |
| AGCGAGCCTTACATGACTGG+CGG | + | chr4.1:31951078-31951097 | MS.gene45464:CDS | 55.0% | |
| AGGCCCCCAATCTCCTAACT+TGG | + | chr4.1:31952002-31952021 | MS.gene45464:CDS | 55.0% | |
| CTGTACCCGCCAGTCATGTA+AGG | - | chr4.1:31951201-31951220 | None:intergenic | 55.0% | |
| GGCGGGTACAGACTTGCATA+CGG | + | chr4.1:31950982-31951001 | MS.gene45464:CDS | 55.0% | |
| GGCGGGTACAGACTTGCATA+TGG | + | chr4.1:31950982-31951001 | MS.gene45464:CDS | 55.0% | |
| GGGGCCTTCTAGATCGTTTG+AGG | - | chr4.1:31951989-31952008 | None:intergenic | 55.0% | |
| GTGAGCCTTACATGACTGGC+GGG | + | chr4.1:31951193-31951212 | MS.gene45464:CDS | 55.0% | |
| ! | CGTTTGAGGTGGTAGATGGG+GGG | - | chr4.1:31951975-31951994 | None:intergenic | 55.0% |
| ! | TGGTAGATGGGGGGCCTTTT+GGG | - | chr4.1:31951966-31951985 | None:intergenic | 55.0% |
| GCGAGCCTTACATGACTGGC+GGG | + | chr4.1:31951079-31951098 | MS.gene45464:CDS | 60.0% | |
| ! | GTGGTAGATGGGGGGCCTTT+TGG | - | chr4.1:31951967-31951986 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 31950976 | 31952309 | 31950976 | ID=MS.gene45464 |
| chr4.1 | mRNA | 31950976 | 31952309 | 31950976 | ID=MS.gene45464.t1;Parent=MS.gene45464 |
| chr4.1 | exon | 31950976 | 31951822 | 31950976 | ID=MS.gene45464.t1.exon1;Parent=MS.gene45464.t1 |
| chr4.1 | CDS | 31950976 | 31951822 | 31950976 | ID=cds.MS.gene45464.t1;Parent=MS.gene45464.t1 |
| chr4.1 | exon | 31951917 | 31952309 | 31951917 | ID=MS.gene45464.t1.exon2;Parent=MS.gene45464.t1 |
| chr4.1 | CDS | 31951917 | 31952309 | 31951917 | ID=cds.MS.gene45464.t1;Parent=MS.gene45464.t1 |
| Gene Sequence |
| Protein sequence |
