AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene052223


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene052223.t1 MTR_3g087540 100.000 160 0 0 1 160 1 160 6.30e-120 335
MS.gene052223.t1 MTR_3g073990 39.189 148 82 3 11 152 8 153 2.20e-31 110
MS.gene052223.t1 MTR_2g016020 32.704 159 96 7 1 152 1 155 1.53e-22 88.6
MS.gene052223.t1 MTR_2g077940 29.688 128 85 3 24 151 42 164 4.90e-13 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene052223.t1 AT5G10130 46.914 162 81 3 1 159 1 160 6.59e-46 147
MS.gene052223.t1 AT4G08685 40.000 165 87 4 1 159 1 159 2.60e-36 123
MS.gene052223.t1 AT1G78040 36.095 169 92 5 1 159 1 163 1.60e-34 119
MS.gene052223.t1 AT1G78040 36.095 169 92 5 1 159 1 163 1.60e-34 119
MS.gene052223.t1 AT1G78040 36.095 169 92 5 1 159 1 163 1.60e-34 119
MS.gene052223.t1 AT1G29140 35.669 157 92 3 4 152 6 161 2.48e-26 98.2
MS.gene052223.t1 AT5G45880 39.024 123 70 3 25 143 35 156 4.26e-23 90.1
MS.gene052223.t1 AT4G18596 33.117 154 90 4 1 142 1 153 1.80e-22 88.2
MS.gene052223.t1 AT4G18596 33.117 154 90 4 1 142 47 199 4.57e-22 88.6

Find 45 sgRNAs with CRISPR-Local

Find 53 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGGATTGTGTTGCTGTTAA+TGG 0.278593 3.2:-74673232 MS.gene052223:CDS
AATAAACACAACCTTTGATA+TGG 0.316996 3.2:+74673163 None:intergenic
GTGTATTACACGATGATATT+GGG 0.373900 3.2:+74672713 None:intergenic
GGTGTATTACACGATGATAT+TGG 0.406710 3.2:+74672712 None:intergenic
CACTGGAACATACAACATAA+TGG 0.429786 3.2:-74672785 MS.gene052223:CDS
CGTCCATAGTCTTCCTGTCT+TGG 0.431661 3.2:+74672839 None:intergenic
ACAAGTAATATTTGAGGAGT+TGG 0.437901 3.2:+74672566 None:intergenic
TAAAGGGAGGATAGAGATGA+GGG 0.446926 3.2:+74673203 None:intergenic
AAATGCTATGGGTTACTTCA+AGG 0.456909 3.2:-74672614 MS.gene052223:CDS
TAGGTGCAAAGGTGGGAATT+CGG 0.460306 3.2:-74672864 MS.gene052223:CDS
TGGGAATTCGGTGCCAAGAC+AGG 0.469322 3.2:-74672852 MS.gene052223:CDS
TATTGTGATACTTGCCGTGC+TGG 0.471219 3.2:-74673144 MS.gene052223:CDS
GTGCCAAGACAGGAAGACTA+TGG 0.475430 3.2:-74672842 MS.gene052223:CDS
TCTTCACTATGCAAATGCTA+TGG 0.481660 3.2:-74672626 MS.gene052223:CDS
TAGCATTTGCATAGTGAAGA+TGG 0.484441 3.2:+74672629 None:intergenic
GATCTGTTACGCCCAGGGTC+CGG 0.486304 3.2:+74672691 None:intergenic
AACATAGCTGTGGCAAGTAA+AGG 0.486675 3.2:+74673186 None:intergenic
ACATAGCTGTGGCAAGTAAA+GGG 0.489307 3.2:+74673187 None:intergenic
GGAGGATAGAGATGAGGGAA+AGG 0.493157 3.2:+74673208 None:intergenic
AGGAGTTGGTGGCAATATTG+AGG 0.508425 3.2:+74672580 None:intergenic
AATCAGACAAGTAATATTTG+AGG 0.510401 3.2:+74672560 None:intergenic
TGCTGGATCTGTTACGCCCA+GGG 0.512778 3.2:+74672686 None:intergenic
AGTAATATTTGAGGAGTTGG+TGG 0.513246 3.2:+74672569 None:intergenic
GTAGTGAGTGAGCACAATGC+TGG 0.521929 3.2:+74672669 None:intergenic
GAGGATAGAGATGAGGGAAA+GGG 0.521934 3.2:+74673209 None:intergenic
ATGCTGGATCTGTTACGCCC+AGG 0.535067 3.2:+74672685 None:intergenic
CTTCACTATGCAAATGCTAT+GGG 0.535954 3.2:-74672625 MS.gene052223:CDS
TCGTGTAATACACCGGACCC+TGG 0.543647 3.2:-74672703 MS.gene052223:CDS
TTGCATAGTGAAGATGGTTG+AGG 0.549905 3.2:+74672635 None:intergenic
TTCTTGATTAGGTGCAAAGG+TGG 0.550705 3.2:-74672872 MS.gene052223:intron
CGTGTAATACACCGGACCCT+GGG 0.556675 3.2:-74672702 MS.gene052223:CDS
TGGTGGCAATATTGAGGCAA+AGG 0.557338 3.2:+74672586 None:intergenic
CATTATGTTGTATGTTCCAG+TGG 0.561706 3.2:+74672786 None:intergenic
GTAAAGGGAGGATAGAGATG+AGG 0.562442 3.2:+74673202 None:intergenic
CATTGCTAATCATCATGGCA+AGG 0.587013 3.2:-74673251 None:intergenic
CGATTCTTGATTAGGTGCAA+AGG 0.587702 3.2:-74672875 MS.gene052223:intron
ACAGCTATGTTCCATATCAA+AGG 0.587960 3.2:-74673174 MS.gene052223:CDS
GAAGGAGTGACAGATTCCAC+TGG 0.617675 3.2:-74672802 MS.gene052223:CDS
TTGGATGTAAAATGTGGCGT+TGG 0.634933 3.2:+74673113 None:intergenic
CGTTGGTTTCGAATCCAGCA+CGG 0.637233 3.2:+74673130 None:intergenic
TCTTGATTAGGTGCAAAGGT+GGG 0.642740 3.2:-74672871 MS.gene052223:intron
AATATCATCGTGTAATACAC+CGG 0.667061 3.2:-74672710 MS.gene052223:CDS
TTTACCTTGGATGTAAAATG+TGG 0.673598 3.2:+74673107 None:intergenic
TTTGATATGGAACATAGCTG+TGG 0.697489 3.2:+74673176 None:intergenic
TAGCTGTGGCAAGTAAAGGG+AGG 0.702643 3.2:+74673190 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATGTAAAAAATTTACGTTA+AGG + chr3.2:74672711-74672730 None:intergenic 15.0%
! AATAAACACAACCTTTGATA+TGG + chr3.2:74672626-74672645 None:intergenic 25.0%
! AATCAGACAAGTAATATTTG+AGG + chr3.2:74673229-74673248 None:intergenic 25.0%
AATATCATCGTGTAATACAC+CGG - chr3.2:74673076-74673095 MS.gene052223:intron 30.0%
ACAAGTAATATTTGAGGAGT+TGG + chr3.2:74673223-74673242 None:intergenic 30.0%
GTGTATTACACGATGATATT+GGG + chr3.2:74673076-74673095 None:intergenic 30.0%
TATTGTGACGATTCTTGATT+AGG - chr3.2:74672903-74672922 MS.gene052223:intron 30.0%
TGTAAAATGTACACATGATC+AGG + chr3.2:74672782-74672801 None:intergenic 30.0%
TTTACCTTGGATGTAAAATG+TGG + chr3.2:74672682-74672701 None:intergenic 30.0%
! CAGTTTTGCAAATATTGTCA+TGG + chr3.2:74673040-74673059 None:intergenic 30.0%
ACAGCTATGTTCCATATCAA+AGG - chr3.2:74672612-74672631 MS.gene052223:CDS 35.0%
AGTAATATTTGAGGAGTTGG+TGG + chr3.2:74673220-74673239 None:intergenic 35.0%
CACTGGAACATACAACATAA+TGG - chr3.2:74673001-74673020 MS.gene052223:intron 35.0%
CATTATGTTGTATGTTCCAG+TGG + chr3.2:74673003-74673022 None:intergenic 35.0%
CTTCACTATGCAAATGCTAT+GGG - chr3.2:74673161-74673180 MS.gene052223:CDS 35.0%
GGTGTATTACACGATGATAT+TGG + chr3.2:74673077-74673096 None:intergenic 35.0%
GTTAAGGTGTGTATTTACCT+TGG + chr3.2:74672695-74672714 None:intergenic 35.0%
TAGCATTTGCATAGTGAAGA+TGG + chr3.2:74673160-74673179 None:intergenic 35.0%
TCTTCACTATGCAAATGCTA+TGG - chr3.2:74673160-74673179 MS.gene052223:CDS 35.0%
TTTGATATGGAACATAGCTG+TGG + chr3.2:74672613-74672632 None:intergenic 35.0%
! AAATGCTATGGGTTACTTCA+AGG - chr3.2:74673172-74673191 MS.gene052223:CDS 35.0%
! AAGGATTGTGTTGCTGTTAA+TGG - chr3.2:74672554-74672573 MS.gene052223:CDS 35.0%
! TATGGACGAAGTTTTCTACA+CGG - chr3.2:74672962-74672981 MS.gene052223:intron 35.0%
AACATAGCTGTGGCAAGTAA+AGG + chr3.2:74672603-74672622 None:intergenic 40.0%
ACATAGCTGTGGCAAGTAAA+GGG + chr3.2:74672602-74672621 None:intergenic 40.0%
CGATTCTTGATTAGGTGCAA+AGG - chr3.2:74672911-74672930 MS.gene052223:intron 40.0%
GTGCTCACTCACTACAAAAA+TGG - chr3.2:74673125-74673144 MS.gene052223:CDS 40.0%
TAAAGGGAGGATAGAGATGA+GGG + chr3.2:74672586-74672605 None:intergenic 40.0%
TCTTGATTAGGTGCAAAGGT+GGG - chr3.2:74672915-74672934 MS.gene052223:intron 40.0%
TTCTTGATTAGGTGCAAAGG+TGG - chr3.2:74672914-74672933 MS.gene052223:intron 40.0%
TTGCATAGTGAAGATGGTTG+AGG + chr3.2:74673154-74673173 None:intergenic 40.0%
TTGGATGTAAAATGTGGCGT+TGG + chr3.2:74672676-74672695 None:intergenic 40.0%
! AACGCCACATTTTACATCCA+AGG - chr3.2:74672675-74672694 MS.gene052223:CDS 40.0%
GAGGATAGAGATGAGGGAAA+GGG + chr3.2:74672580-74672599 None:intergenic 45.0%
GTAAAGGGAGGATAGAGATG+AGG + chr3.2:74672587-74672606 None:intergenic 45.0%
TAGGTGCAAAGGTGGGAATT+CGG - chr3.2:74672922-74672941 MS.gene052223:intron 45.0%
TATTGTGATACTTGCCGTGC+TGG - chr3.2:74672642-74672661 MS.gene052223:CDS 45.0%
TGGTGGCAATATTGAGGCAA+AGG + chr3.2:74673203-74673222 None:intergenic 45.0%
! GACGAAGTTTTCTACACGGA+AGG - chr3.2:74672966-74672985 MS.gene052223:intron 45.0%
!! AGGAGTTGGTGGCAATATTG+AGG + chr3.2:74673209-74673228 None:intergenic 45.0%
CGTCCATAGTCTTCCTGTCT+TGG + chr3.2:74672950-74672969 None:intergenic 50.0%
GAAGGAGTGACAGATTCCAC+TGG - chr3.2:74672984-74673003 MS.gene052223:intron 50.0%
GGAGGATAGAGATGAGGGAA+AGG + chr3.2:74672581-74672600 None:intergenic 50.0%
GTAGTGAGTGAGCACAATGC+TGG + chr3.2:74673120-74673139 None:intergenic 50.0%
GTGCCAAGACAGGAAGACTA+TGG - chr3.2:74672944-74672963 MS.gene052223:intron 50.0%
TAGCTGTGGCAAGTAAAGGG+AGG + chr3.2:74672599-74672618 None:intergenic 50.0%
!! CGTTGGTTTCGAATCCAGCA+CGG + chr3.2:74672659-74672678 None:intergenic 50.0%
ATGCTGGATCTGTTACGCCC+AGG + chr3.2:74673104-74673123 None:intergenic 55.0%
CGTGTAATACACCGGACCCT+GGG - chr3.2:74673084-74673103 MS.gene052223:intron 55.0%
TCGTGTAATACACCGGACCC+TGG - chr3.2:74673083-74673102 MS.gene052223:intron 55.0%
TGCTGGATCTGTTACGCCCA+GGG + chr3.2:74673103-74673122 None:intergenic 55.0%
!! TGGGAATTCGGTGCCAAGAC+AGG - chr3.2:74672934-74672953 MS.gene052223:intron 55.0%
GATCTGTTACGCCCAGGGTC+CGG + chr3.2:74673098-74673117 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr3.2 gene 74672549 74673259 74672549 ID=MS.gene052223
chr3.2 mRNA 74672549 74673259 74672549 ID=MS.gene052223.t1;Parent=MS.gene052223
chr3.2 exon 74673112 74673259 74673112 ID=MS.gene052223.t1.exon1;Parent=MS.gene052223.t1
chr3.2 CDS 74673112 74673259 74673112 ID=cds.MS.gene052223.t1;Parent=MS.gene052223.t1
chr3.2 exon 74672549 74672883 74672549 ID=MS.gene052223.t1.exon2;Parent=MS.gene052223.t1
chr3.2 CDS 74672549 74672883 74672549 ID=cds.MS.gene052223.t1;Parent=MS.gene052223.t1
Gene Sequence

>MS.gene052223

ATGGCAAGGATTGTGTTGCTGTTAATGGCCCTTTCCCTCATCTCTATCCTCCCTTTACTTGCCACAGCTATGTTCCATATCAAAGGTTGTGTTTATTGTGATACTTGCCGTGCTGGATTCGAAACCAACGCCACATTTTACATCCAAGGTAAATACACACCTTAACGTAAATTTTTTACATTGTCTATGATTGAAGTCAAACATTTTAATGATCAGATGTATGTTTTACACCTGATCATGTGTACATTTTACACATCACTTTTTACATCTTAGCGTAGAATGTTTTAGAACGTCTATACATACAAATTAAATCATTTCTAATTTAATGTTTTCTTACAATTGATGTGTGTATGTTATTGTGACGATTCTTGATTAGGTGCAAAGGTGGGAATTCGGTGCCAAGACAGGAAGACTATGGACGAAGTTTTCTACACGGAAGGAGTGACAGATTCCACTGGAACATACAACATAATGGTTGAGAATGATCACCATGACAATATTTGCAAAACTGTGCTTGTTAGCAGCCCAATATCATCGTGTAATACACCGGACCCTGGGCGTAACAGATCCAGCATTGTGCTCACTCACTACAAAAATGGTGTCCTCAACCATCTTCACTATGCAAATGCTATGGGTTACTTCAAGGATGATCCTTTGCCTCAATATTGCCACCAACTCCTCAAATATTACTTGTCTGATTCAGATGTTTGA

Protein sequence

>MS.gene052223.t1

MARIVLLLMALSLISILPLLATAMFHIKGCVYCDTCRAGFETNATFYIQGAKVGIRCQDRKTMDEVFYTEGVTDSTGTYNIMVENDHHDNICKTVLVSSPISSCNTPDPGRNRSSIVLTHYKNGVLNHLHYANAMGYFKDDPLPQYCHQLLKYYLSDSDV