AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene45497

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene45497.t1 MTR_2g096900 96.552 145 5 0 1 145 1 145 6.03e-98 286
MS.gene45497.t1 MTR_2g096920 86.014 143 20 0 1 143 1 143 1.47e-73 224
MS.gene45497.t1 MTR_2g096920 88.000 100 12 0 1 100 1 100 5.58e-58 182
MS.gene45497.t1 MTR_1g093780 44.211 190 88 5 149 325 331 515 2.29e-33 129
MS.gene45497.t1 MTR_4g121900 33.766 154 75 2 18 144 24 177 1.74e-20 87.4
MS.gene45497.t1 MTR_2g034550 33.548 155 75 3 18 144 26 180 3.22e-18 81.3
MS.gene45497.t1 MTR_2g437210 43.810 105 50 3 224 325 123 221 1.31e-14 72.4
MS.gene45497.t1 MTR_7g083320 43.750 128 43 5 203 324 1 105 3.58e-14 68.2
MS.gene45497.t1 MTR_2g074890 38.255 149 69 7 184 325 345 477 4.39e-12 67.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene45497.t1 AT2G28900 62.238 143 54 0 1 143 1 143 4.06e-59 187
MS.gene45497.t1 AT4G16160 32.026 153 77 3 18 143 21 173 8.79e-18 80.1
MS.gene45497.t1 AT4G16160 31.613 155 77 3 18 143 21 175 1.24e-17 79.7

Find 97 sgRNAs with CRISPR-Local

Find 195 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCAGATAATGAAGGAATTT+TGG 0.142163 2.1:+6776603 MS.gene45497:CDS
ACATGACAGTCACAAATATT+TGG 0.169688 2.1:-6776852 None:intergenic
CCTTTGCCAATGTGAGTCTT+AGG 0.217782 2.1:-6776761 None:intergenic
ATGCCTCATAGCAGCATTTC+AGG 0.220503 2.1:+6774309 MS.gene45497:CDS
CTTCTCAACCTCACTGTTGA+TGG 0.269257 2.1:+6774381 MS.gene45497:CDS
CATCCAAACTCGTTCCAAAA+AGG 0.284341 2.1:+6776811 MS.gene45497:CDS
GCTGGCTGCTGAGTGGTTAC+TGG 0.311741 2.1:-6776570 None:intergenic
AGTCTGGGCAAAAGAGTTAT+AGG 0.327469 2.1:+6776530 MS.gene45497:CDS
ATTGTAGTAGGTGCCATTAC+AGG 0.330295 2.1:+6775822 MS.gene45497:CDS
ACTGCTGCAGCATTCATAAA+TGG 0.345573 2.1:+6775858 MS.gene45497:CDS
GGAACAGCCAAAGACAAAAC+TGG 0.362261 2.1:-6776482 None:intergenic
CATGACAGTCACAAATATTT+GGG 0.363803 2.1:-6776851 None:intergenic
CATTTAAAGAAGGTGCATAC+TGG 0.364036 2.1:+6775273 MS.gene45497:CDS
GTTGATGGTTTCTTGAAGAT+TGG 0.388016 2.1:+6774396 MS.gene45497:CDS
CTTAGTCTTCCTACCAGGCT+TGG 0.394054 2.1:-6776726 None:intergenic
CTCAGACATTAACACTGATA+TGG 0.403641 2.1:+6776652 MS.gene45497:CDS
CAAATGTTGGACTCTGTCTC+GGG 0.404366 2.1:+6776416 MS.gene45497:CDS
CGTGGATGAAGATTTGAGTC+TGG 0.409368 2.1:+6776514 MS.gene45497:CDS
AGATGCTATGTCAGTATGTT+TGG 0.419644 2.1:-6776699 None:intergenic
GGATCCGTGGCACCAGAGAC+TGG 0.423846 2.1:+6775432 MS.gene45497:CDS
ACATTTGGAGGTGCAGTGAC+AGG 0.426302 2.1:+6775759 MS.gene45497:CDS
TTGGACTCTGTCTCGGGGAG+GGG 0.430301 2.1:+6776422 MS.gene45497:CDS
GAACACCGAACCACCAAACT+TGG 0.430785 2.1:-6776452 None:intergenic
GTATATGTTGGAACAGAATA+CGG 0.434501 2.1:+6775401 MS.gene45497:CDS
CTTTGCCAATGTGAGTCTTA+GGG 0.439223 2.1:-6776760 None:intergenic
CGCAATTGCAGCCCCTGTAA+TGG 0.440225 2.1:-6775835 None:intergenic
AGGTGCAGTGACAGGGGCTC+TGG 0.442203 2.1:+6775767 MS.gene45497:CDS
TGCTTCTTAGTCTTCCTACC+AGG 0.442253 2.1:-6776731 None:intergenic
AGAGTTATAGGTGGAAAGTC+TGG 0.443810 2.1:+6776542 MS.gene45497:CDS
CACCTATTCCATTGGAATCA+AGG 0.445195 2.1:+6776360 MS.gene45497:CDS
GTTGGACTCTGTCTCGGGGA+GGG 0.450789 2.1:+6776421 MS.gene45497:CDS
TGGACTCTGTCTCGGGGAGG+GGG 0.451579 2.1:+6776423 MS.gene45497:CDS
GTGATCCAAGTTTGGTGGTT+CGG 0.457160 2.1:+6776447 MS.gene45497:CDS
GCAAATGTTGGACTCTGTCT+CGG 0.470535 2.1:+6776415 MS.gene45497:CDS
ATTTGCTTGTTAGAAACTAT+AGG 0.472776 2.1:-6776398 None:intergenic
GGCCTTGATTCCAATGGAAT+AGG 0.478081 2.1:-6776362 None:intergenic
ATCTTCATCCACGGAAGTGA+CGG 0.484083 2.1:-6776504 None:intergenic
ACCCTTTCGAGCAATATGAT+AGG 0.487140 2.1:-6774541 None:intergenic
TGAGAGAATTACCCAGTCTC+TGG 0.488901 2.1:-6775444 None:intergenic
GTCAATGACCACATCGACCT+TGG 0.493655 2.1:-6774346 None:intergenic
ATTTAAAGAAGGTGCATACT+GGG 0.494694 2.1:+6775274 MS.gene45497:CDS
ACCTATCATATTGCTCGAAA+GGG 0.494769 2.1:+6774540 MS.gene45497:CDS
AAGGCAGACAAGATTGTAGT+AGG 0.498217 2.1:+6775810 MS.gene45497:CDS
GCTATAGCTGGTGTATATGT+TGG 0.502697 2.1:+6775389 MS.gene45497:CDS
CTGCAGCATTCATAAATGGC+TGG 0.512276 2.1:+6775862 MS.gene45497:CDS
TACCTATCATATTGCTCGAA+AGG 0.512817 2.1:+6774539 MS.gene45497:CDS
ATCTGCAGCCAACAATGAAA+AGG 0.513448 2.1:+6775791 MS.gene45497:CDS
GTGGATGAAGATTTGAGTCT+GGG 0.514738 2.1:+6776515 MS.gene45497:CDS
GGCTGTTCCCGTCACTTCCG+TGG 0.514928 2.1:+6776496 MS.gene45497:CDS
TCAATGACCACATCGACCTT+GGG 0.517343 2.1:-6774345 None:intergenic
TTGTAGTAGGTGCCATTACA+GGG 0.523008 2.1:+6775823 MS.gene45497:CDS
AGTTTCTAACAAGCAAATGT+TGG 0.526698 2.1:+6776403 MS.gene45497:CDS
GCAAGATGCACCTATTCCAT+TGG 0.528869 2.1:+6776352 MS.gene45497:CDS
AGGATCAGTGTCTTCTCCCA+AGG 0.530325 2.1:+6774329 MS.gene45497:CDS
TATATGTTGGAACAGAATAC+GGG 0.530549 2.1:+6775402 MS.gene45497:CDS
GAACAGCCAAAGACAAAACT+GGG 0.536588 2.1:-6776481 None:intergenic
CACATTGGCAAAGGTTCTGA+TGG 0.538523 2.1:+6776770 MS.gene45497:CDS
GAGGATGCTTGCTTAGAGCC+AGG 0.540490 2.1:+6776323 MS.gene45497:intron
CATCTAACACCAAGCCTGGT+AGG 0.540647 2.1:+6776717 MS.gene45497:CDS
CATTTGGAGGTGCAGTGACA+GGG 0.546047 2.1:+6775760 MS.gene45497:CDS
TTGTTAGAAACTATAGGTAG+AGG 0.547056 2.1:-6776392 None:intergenic
TAAAAGTGTGCAGATAATGA+AGG 0.550725 2.1:+6776595 MS.gene45497:CDS
ATAGCATCTAACACCAAGCC+TGG 0.555962 2.1:+6776713 MS.gene45497:CDS
ACGCTTACAGGAGCTATAGC+TGG 0.557663 2.1:+6775377 MS.gene45497:intron
GGTCGATGTGGTCATTGACA+TGG 0.564917 2.1:+6774350 MS.gene45497:CDS
TCTTCATCCACGGAAGTGAC+GGG 0.565252 2.1:-6776503 None:intergenic
GATCCTGAAATGCTGCTATG+AGG 0.565334 2.1:-6774312 None:intergenic
ATCCTCTGCAAGTGCCCTTG+TGG 0.568133 2.1:-6774517 None:intergenic
ACTATAGGTAGAGGCTGAGA+GGG 0.572049 2.1:-6776383 None:intergenic
AACTATAGGTAGAGGCTGAG+AGG 0.577361 2.1:-6776384 None:intergenic
TGTCACTGCACCTCCAAATG+TGG 0.580744 2.1:-6775757 None:intergenic
TGAGAGGGCCTTGATTCCAA+TGG 0.582673 2.1:-6776368 None:intergenic
TGTTGGACTCTGTCTCGGGG+AGG 0.585063 2.1:+6776420 MS.gene45497:CDS
GGGGTGTGATCCAAGTTTGG+TGG 0.586053 2.1:+6776442 MS.gene45497:CDS
CTGAAGAAGACATTTAAAGA+AGG 0.586357 2.1:+6775263 MS.gene45497:CDS
TTACCCAGTCTCTGGTGCCA+CGG 0.593471 2.1:-6775436 None:intergenic
GATCCGTGGCACCAGAGACT+GGG 0.594640 2.1:+6775433 MS.gene45497:CDS
AGCCACAAGGGCACTTGCAG+AGG 0.596020 2.1:+6774515 MS.gene45497:CDS
CATATCAGTGTTAATGTCTG+AGG 0.597268 2.1:-6776651 None:intergenic
AAAAGCCCTAAGACTCACAT+TGG 0.608767 2.1:+6776755 MS.gene45497:CDS
TACGGGGTGGAGAGGATCCG+TGG 0.613293 2.1:+6775419 MS.gene45497:CDS
CCTAAGACTCACATTGGCAA+AGG 0.618293 2.1:+6776761 MS.gene45497:CDS
GTCTTCTCCCAAGGTCGATG+TGG 0.618468 2.1:+6774338 MS.gene45497:CDS
GAACAGAATACGGGGTGGAG+AGG 0.619958 2.1:+6775411 MS.gene45497:CDS
GTCGATGTGGTCATTGACAT+GGG 0.620754 2.1:+6774351 MS.gene45497:CDS
GGTGCATCTTGCTCTAGACC+TGG 0.627077 2.1:-6776341 None:intergenic
CAGACTCAAATCTTCATCCA+CGG 0.636374 2.1:-6776513 None:intergenic
TCAACAGTGAGGTTGAGAAG+TGG 0.636785 2.1:-6774378 None:intergenic
ATTTGGAGGTGCAGTGACAG+GGG 0.640699 2.1:+6775761 MS.gene45497:CDS
CTGGGCAAAAGAGTTATAGG+TGG 0.668810 2.1:+6776533 MS.gene45497:CDS
AAATGTTGGACTCTGTCTCG+GGG 0.672447 2.1:+6776417 MS.gene45497:CDS
TTTAAAGAAGGTGCATACTG+GGG 0.700970 2.1:+6775275 MS.gene45497:CDS
TGTAGTAGGTGCCATTACAG+GGG 0.704939 2.1:+6775824 MS.gene45497:CDS
TCAAGAAACCATCAACAGTG+AGG 0.715759 2.1:-6774389 None:intergenic
TGTTGGAACAGAATACGGGG+TGG 0.732754 2.1:+6775406 MS.gene45497:CDS
AACAGCCAAAGACAAAACTG+GGG 0.763097 2.1:-6776480 None:intergenic
ATATGTTGGAACAGAATACG+GGG 0.764667 2.1:+6775403 MS.gene45497:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATATTAAAAATTATATTTT+AGG - chr2.1:6774834-6774853 None:intergenic 0.0%
!!! AAATTATATTTTAGGAGTAA+AGG - chr2.1:6774826-6774845 None:intergenic 15.0%
!! AAAAGATGAAAACTTTGATT+CGG + chr2.1:6774608-6774627 MS.gene45497:intron 20.0%
!! AACTTAAATTGAACAATAGA+AGG - chr2.1:6775677-6775696 None:intergenic 20.0%
!! ATATATATCATGATTTCAAC+AGG - chr2.1:6776210-6776229 None:intergenic 20.0%
!! CATCAAAATTATTACATAAC+CGG - chr2.1:6775974-6775993 None:intergenic 20.0%
!! TATATATCATGATTTCAACA+GGG - chr2.1:6776209-6776228 None:intergenic 20.0%
!! TATTATTCCATGTTTGAAAA+GGG + chr2.1:6775917-6775936 MS.gene45497:intron 20.0%
!! TATTTATCAAAGTTAAAGCA+TGG - chr2.1:6775062-6775081 None:intergenic 20.0%
!!! AAATGTGTTAACTTTGTTTT+AGG + chr2.1:6775639-6775658 MS.gene45497:intron 20.0%
!!! ATGCTTTAACTTTGATAAAT+AGG + chr2.1:6775061-6775080 MS.gene45497:intron 20.0%
!!! CGTTTTCATAAATATTATTG+CGG + chr2.1:6776136-6776155 MS.gene45497:intron 20.0%
!!! TTATCATGATTGATTCATTT+GGG + chr2.1:6774796-6774815 MS.gene45497:intron 20.0%
!!! TTCAGTATTTTTTAAAGCAA+CGG - chr2.1:6776248-6776267 None:intergenic 20.0%
!!! TTTTGGAGATTTTAACAAAA+GGG - chr2.1:6774592-6774611 None:intergenic 20.0%
! AAAGATGAAAACTTTGATTC+GGG + chr2.1:6774609-6774628 MS.gene45497:intron 25.0%
! AGACAAAATGTCTATTGAAT+GGG - chr2.1:6775198-6775217 None:intergenic 25.0%
! AGAGATTAGATGTCATTATA+TGG - chr2.1:6775556-6775575 None:intergenic 25.0%
! ATTATTCCATGTTTGAAAAG+GGG + chr2.1:6775918-6775937 MS.gene45497:intron 25.0%
! CAATAATGTGATTTGAACTT+GGG + chr2.1:6774915-6774934 MS.gene45497:intron 25.0%
! CTATTATTCCATGTTTGAAA+AGG + chr2.1:6775916-6775935 MS.gene45497:intron 25.0%
! GAAAACACTTAAAATAGACT+TGG + chr2.1:6775478-6775497 MS.gene45497:intron 25.0%
! TAGACAAAATGTCTATTGAA+TGG - chr2.1:6775199-6775218 None:intergenic 25.0%
!! ACTTTTAGTATAAAGAAACC+TGG + chr2.1:6776060-6776079 MS.gene45497:intron 25.0%
!! ATTTGCTTGTTAGAAACTAT+AGG - chr2.1:6776401-6776420 None:intergenic 25.0%
!! CGGTTATGTAATAATTTTGA+TGG + chr2.1:6775972-6775991 MS.gene45497:intron 25.0%
!! CTTATCATGATTGATTCATT+TGG + chr2.1:6774795-6774814 MS.gene45497:intron 25.0%
!! CTTTTAGTATAAAGAAACCT+GGG + chr2.1:6776061-6776080 MS.gene45497:intron 25.0%
!! GAATCAAAGTTTTCATCTTT+TGG - chr2.1:6774609-6774628 None:intergenic 25.0%
!! TATCATGATTGATTCATTTG+GGG + chr2.1:6774797-6774816 MS.gene45497:intron 25.0%
!!! CTTTTGGAGATTTTAACAAA+AGG - chr2.1:6774593-6774612 None:intergenic 25.0%
AAATGAGAGAATCCGAATTT+AGG - chr2.1:6774978-6774997 None:intergenic 30.0%
AAATTGAACAATAGAAGGCA+TGG - chr2.1:6775672-6775691 None:intergenic 30.0%
AAATTTAAACATTAGCAGCC+AGG + chr2.1:6776099-6776118 MS.gene45497:intron 30.0%
AACGTTAAACTTTATTCACC+TGG - chr2.1:6776120-6776139 None:intergenic 30.0%
AATGAGAGAATCCGAATTTA+GGG - chr2.1:6774977-6774996 None:intergenic 30.0%
AGTTTCTAACAAGCAAATGT+TGG + chr2.1:6776403-6776422 MS.gene45497:CDS 30.0%
CTGAAGAAGACATTTAAAGA+AGG + chr2.1:6775263-6775282 MS.gene45497:CDS 30.0%
GAGAATCTAAATCCTGAAAA+GGG + chr2.1:6775165-6775184 MS.gene45497:intron 30.0%
GCAATAATGTGATTTGAACT+TGG + chr2.1:6774914-6774933 MS.gene45497:intron 30.0%
GTATATGTTGGAACAGAATA+CGG + chr2.1:6775401-6775420 MS.gene45497:CDS 30.0%
TATATGTTGGAACAGAATAC+GGG + chr2.1:6775402-6775421 MS.gene45497:CDS 30.0%
TCCTAGAAAAGAATAATGAC+AGG + chr2.1:6774946-6774965 MS.gene45497:intron 30.0%
TGAGAATCTAAATCCTGAAA+AGG + chr2.1:6775164-6775183 MS.gene45497:intron 30.0%
TTGAGCTGTTTGATCAAAAT+CGG + chr2.1:6775031-6775050 MS.gene45497:intron 30.0%
TTGTTAGAAACTATAGGTAG+AGG - chr2.1:6776395-6776414 None:intergenic 30.0%
TTTCTGTGTATATCCTAAAG+AGG + chr2.1:6776304-6776323 MS.gene45497:intron 30.0%
! ATTTAAAGAAGGTGCATACT+GGG + chr2.1:6775274-6775293 MS.gene45497:CDS 30.0%
! CAGATAATGAAGGAATTTTG+GGG + chr2.1:6776605-6776624 MS.gene45497:CDS 30.0%
! GCAGATAATGAAGGAATTTT+GGG + chr2.1:6776604-6776623 MS.gene45497:CDS 30.0%
! TAAAAGTGTGCAGATAATGA+AGG + chr2.1:6776595-6776614 MS.gene45497:CDS 30.0%
! TCATTATCTGCACACTTTTA+AGG - chr2.1:6776595-6776614 None:intergenic 30.0%
! TCCTGTCATTATTCTTTTCT+AGG - chr2.1:6774950-6774969 None:intergenic 30.0%
! TGCAGATAATGAAGGAATTT+TGG + chr2.1:6776603-6776622 MS.gene45497:CDS 30.0%
AACAAGACCTCTCATTAACA+AGG - chr2.1:6775239-6775258 None:intergenic 35.0%
ACAAGGAACAAAGCATATCA+TGG - chr2.1:6775222-6775241 None:intergenic 35.0%
ACCTATCATATTGCTCGAAA+GGG + chr2.1:6774540-6774559 MS.gene45497:CDS 35.0%
ACTGACATATCAAGATCAGA+TGG - chr2.1:6775112-6775131 None:intergenic 35.0%
ACTGGGTAATTCTCTCAATA+GGG + chr2.1:6775450-6775469 MS.gene45497:intron 35.0%
AGATGCTATGTCAGTATGTT+TGG - chr2.1:6776702-6776721 None:intergenic 35.0%
ATATGTTGGAACAGAATACG+GGG + chr2.1:6775403-6775422 MS.gene45497:CDS 35.0%
ATCCAAACTCGTTCCAAAAA+GGG + chr2.1:6776812-6776831 MS.gene45497:CDS 35.0%
ATGAGAGAATCCGAATTTAG+GGG - chr2.1:6774976-6774995 None:intergenic 35.0%
CAAAAGGGTTATAGCAAAAG+TGG - chr2.1:6774577-6774596 None:intergenic 35.0%
CATATCAGTGTTAATGTCTG+AGG - chr2.1:6776654-6776673 None:intergenic 35.0%
CTCAGACATTAACACTGATA+TGG + chr2.1:6776652-6776671 MS.gene45497:CDS 35.0%
CTTTGTTCCTTGTTAATGAG+AGG + chr2.1:6775229-6775248 MS.gene45497:intron 35.0%
GTACAGAAAAATGCCACATT+TGG + chr2.1:6775744-6775763 MS.gene45497:intron 35.0%
TAAATGGCTGGAGTTTCTAA+TGG + chr2.1:6775874-6775893 MS.gene45497:intron 35.0%
TAATGTGATTTGAACTTGGG+AGG + chr2.1:6774918-6774937 MS.gene45497:intron 35.0%
TACCTATCATATTGCTCGAA+AGG + chr2.1:6774539-6774558 MS.gene45497:CDS 35.0%
TCTGTACATCAAAAGACCAA+GGG - chr2.1:6775731-6775750 None:intergenic 35.0%
TTCAGCTTTCTGTCAATTCT+TGG + chr2.1:6776017-6776036 MS.gene45497:intron 35.0%
TTCTAGATCATGAGATGAAG+GGG + chr2.1:6776159-6776178 MS.gene45497:intron 35.0%
TTCTGTACATCAAAAGACCA+AGG - chr2.1:6775732-6775751 None:intergenic 35.0%
TTGGTATGTATGCATCTTTG+AGG + chr2.1:6774892-6774911 MS.gene45497:intron 35.0%
! ATTTTCCTTTCTACGCTTAC+AGG + chr2.1:6775365-6775384 MS.gene45497:intron 35.0%
! CATTTAAAGAAGGTGCATAC+TGG + chr2.1:6775273-6775292 MS.gene45497:CDS 35.0%
! GTTCTAGATCATGAGATGAA+GGG + chr2.1:6776158-6776177 MS.gene45497:intron 35.0%
! TTCTGTCAATTCTTGGTCTT+TGG + chr2.1:6776024-6776043 MS.gene45497:intron 35.0%
! TTTAAAGAAGGTGCATACTG+GGG + chr2.1:6775275-6775294 MS.gene45497:CDS 35.0%
!! GCTTTTGATTACACCCTTTT+TGG - chr2.1:6776828-6776847 None:intergenic 35.0%
!! GTTGATGGTTTCTTGAAGAT+TGG + chr2.1:6774396-6774415 MS.gene45497:CDS 35.0%
!! TAGAGAGAGCACTTTATTTC+TGG + chr2.1:6775521-6775540 MS.gene45497:intron 35.0%
AAAAGCCCTAAGACTCACAT+TGG + chr2.1:6776755-6776774 MS.gene45497:CDS 40.0%
AACAGCCAAAGACAAAACTG+GGG - chr2.1:6776483-6776502 None:intergenic 40.0%
AAGGCAGACAAGATTGTAGT+AGG + chr2.1:6775810-6775829 MS.gene45497:CDS 40.0%
ACCCTTTCGAGCAATATGAT+AGG - chr2.1:6774544-6774563 None:intergenic 40.0%
ACTGCTGCAGCATTCATAAA+TGG + chr2.1:6775858-6775877 MS.gene45497:CDS 40.0%
AGAGTTATAGGTGGAAAGTC+TGG + chr2.1:6776542-6776561 MS.gene45497:CDS 40.0%
AGTCTGGGCAAAAGAGTTAT+AGG + chr2.1:6776530-6776549 MS.gene45497:CDS 40.0%
ATCTAAATCCTGAAAAGGGC+AGG + chr2.1:6775169-6775188 MS.gene45497:intron 40.0%
ATCTGCAGCCAACAATGAAA+AGG + chr2.1:6775791-6775810 MS.gene45497:CDS 40.0%
CACCTATTCCATTGGAATCA+AGG + chr2.1:6776360-6776379 MS.gene45497:CDS 40.0%
CAGAAAAATGCCACATTTGG+AGG + chr2.1:6775747-6775766 MS.gene45497:intron 40.0%
CAGACTCAAATCTTCATCCA+CGG - chr2.1:6776516-6776535 None:intergenic 40.0%
CATCCAAACTCGTTCCAAAA+AGG + chr2.1:6776811-6776830 MS.gene45497:CDS 40.0%
CTTCTAAACATGTCTGTTCC+AGG + chr2.1:6774712-6774731 MS.gene45497:intron 40.0%
CTTTGCCAATGTGAGTCTTA+GGG - chr2.1:6776763-6776782 None:intergenic 40.0%
GAACAGCCAAAGACAAAACT+GGG - chr2.1:6776484-6776503 None:intergenic 40.0%
GACTGGGTAATTCTCTCAAT+AGG + chr2.1:6775449-6775468 MS.gene45497:intron 40.0%
GCTATAGCTGGTGTATATGT+TGG + chr2.1:6775389-6775408 MS.gene45497:CDS 40.0%
GTGGATGAAGATTTGAGTCT+GGG + chr2.1:6776515-6776534 MS.gene45497:CDS 40.0%
TCAAAAGACCAAGGGAACAA+CGG - chr2.1:6775723-6775742 None:intergenic 40.0%
TCAAGAAACCATCAACAGTG+AGG - chr2.1:6774392-6774411 None:intergenic 40.0%
TCTAAGCAAGCATCCTCTTT+AGG - chr2.1:6776320-6776339 None:intergenic 40.0%
TTCACGTAATCACACACTTG+CGG - chr2.1:6775141-6775160 None:intergenic 40.0%
TTGAAAAGGGGTTGCACATT+TGG + chr2.1:6775930-6775949 MS.gene45497:intron 40.0%
! CATGATTGATTCATTTGGGG+TGG + chr2.1:6774800-6774819 MS.gene45497:intron 40.0%
! CTTGTCTGCCTTTTCATTGT+TGG - chr2.1:6775802-6775821 None:intergenic 40.0%
! GTCTCTTTTGTCTGTCTTAC+CGG + chr2.1:6775952-6775971 MS.gene45497:intron 40.0%
!! ATTGTAGTAGGTGCCATTAC+AGG + chr2.1:6775822-6775841 MS.gene45497:CDS 40.0%
!! GGTTCTAGATCATGAGATGA+AGG + chr2.1:6776157-6776176 MS.gene45497:intron 40.0%
!! TTGTAGTAGGTGCCATTACA+GGG + chr2.1:6775823-6775842 MS.gene45497:CDS 40.0%
!!! TATCTGCACACTTTTAAGGC+TGG - chr2.1:6776591-6776610 None:intergenic 40.0%
AAAGAAACCTGGGCATGAAC+AGG + chr2.1:6776071-6776090 MS.gene45497:intron 45.0%
AAATGTTGGACTCTGTCTCG+GGG + chr2.1:6776417-6776436 MS.gene45497:CDS 45.0%
AACTATAGGTAGAGGCTGAG+AGG - chr2.1:6776387-6776406 None:intergenic 45.0%
ACTATAGGTAGAGGCTGAGA+GGG - chr2.1:6776386-6776405 None:intergenic 45.0%
ATAGCATCTAACACCAAGCC+TGG + chr2.1:6776713-6776732 MS.gene45497:CDS 45.0%
ATCTTCATCCACGGAAGTGA+CGG - chr2.1:6776507-6776526 None:intergenic 45.0%
ATGCCTCATAGCAGCATTTC+AGG + chr2.1:6774309-6774328 MS.gene45497:CDS 45.0%
CAAATGTTGGACTCTGTCTC+GGG + chr2.1:6776416-6776435 MS.gene45497:CDS 45.0%
CCTAAGACTCACATTGGCAA+AGG + chr2.1:6776761-6776780 MS.gene45497:CDS 45.0%
CCTTTGCCAATGTGAGTCTT+AGG - chr2.1:6776764-6776783 None:intergenic 45.0%
CGTGGATGAAGATTTGAGTC+TGG + chr2.1:6776514-6776533 MS.gene45497:CDS 45.0%
CTGCAGCATTCATAAATGGC+TGG + chr2.1:6775862-6775881 MS.gene45497:CDS 45.0%
CTGGGCAAAAGAGTTATAGG+TGG + chr2.1:6776533-6776552 MS.gene45497:CDS 45.0%
CTTCTCAACCTCACTGTTGA+TGG + chr2.1:6774381-6774400 MS.gene45497:CDS 45.0%
GACAGGAAAGCCCCTAAATT+CGG + chr2.1:6774963-6774982 MS.gene45497:intron 45.0%
GATCCTGAAATGCTGCTATG+AGG - chr2.1:6774315-6774334 None:intergenic 45.0%
GATTCTTACCGTTGTTCCCT+TGG + chr2.1:6775712-6775731 MS.gene45497:intron 45.0%
GCAAATGTTGGACTCTGTCT+CGG + chr2.1:6776415-6776434 MS.gene45497:CDS 45.0%
GCAAGATGCACCTATTCCAT+TGG + chr2.1:6776352-6776371 MS.gene45497:CDS 45.0%
GGAACAGCCAAAGACAAAAC+TGG - chr2.1:6776485-6776504 None:intergenic 45.0%
GGCCTTGATTCCAATGGAAT+AGG - chr2.1:6776365-6776384 None:intergenic 45.0%
GTCGATGTGGTCATTGACAT+GGG + chr2.1:6774351-6774370 MS.gene45497:CDS 45.0%
TAGCTCCTGTAAGCGTAGAA+AGG - chr2.1:6775373-6775392 None:intergenic 45.0%
TCAACAGTGAGGTTGAGAAG+TGG - chr2.1:6774381-6774400 None:intergenic 45.0%
TCAATGACCACATCGACCTT+GGG - chr2.1:6774348-6774367 None:intergenic 45.0%
TCACTACTTGAGACACTTCC+TGG - chr2.1:6774733-6774752 None:intergenic 45.0%
TGAGAGAATTACCCAGTCTC+TGG - chr2.1:6775447-6775466 None:intergenic 45.0%
TTGAAAACCTGTTCATGCCC+AGG - chr2.1:6776081-6776100 None:intergenic 45.0%
! CACATTGGCAAAGGTTCTGA+TGG + chr2.1:6776770-6776789 MS.gene45497:CDS 45.0%
! GTGATCCAAGTTTGGTGGTT+CGG + chr2.1:6776447-6776466 MS.gene45497:CDS 45.0%
! GTGCAACCCCTTTTCAAACA+TGG - chr2.1:6775927-6775946 None:intergenic 45.0%
! TGCTTCTTAGTCTTCCTACC+AGG - chr2.1:6776734-6776753 None:intergenic 45.0%
!! TAGCACCCCAGTTTTGTCTT+TGG + chr2.1:6776475-6776494 MS.gene45497:CDS 45.0%
!! TGTAGTAGGTGCCATTACAG+GGG + chr2.1:6775824-6775843 MS.gene45497:CDS 45.0%
!!! CACCCTTTTTGGAACGAGTT+TGG - chr2.1:6776817-6776836 None:intergenic 45.0%
!!! TTTTCAGGTTGCAGCCACAA+GGG + chr2.1:6774503-6774522 MS.gene45497:intron 45.0%
!!! TTTTTCAGGTTGCAGCCACA+AGG + chr2.1:6774502-6774521 MS.gene45497:intron 45.0%
!! AAAAATAAAAAATAAATAGT+TGG - chr2.1:6774483-6774502 None:intergenic 5.0%
!! ATATATAAATTACTTATAAT+TGG - chr2.1:6774873-6774892 None:intergenic 5.0%
!! ATTATAAGTAATTTATATAT+TGG + chr2.1:6774873-6774892 MS.gene45497:intron 5.0%
!!! AAATAATAATATATTGATTT+TGG - chr2.1:6774645-6774664 None:intergenic 5.0%
!!! TTATTTTTTATTTTTTTTTC+AGG + chr2.1:6774488-6774507 MS.gene45497:intron 5.0%
AAGGGGACGTGTTGTAAGGT+AGG + chr2.1:6776176-6776195 MS.gene45497:intron 50.0%
ACGCTTACAGGAGCTATAGC+TGG + chr2.1:6775377-6775396 MS.gene45497:intron 50.0%
AGGATCAGTGTCTTCTCCCA+AGG + chr2.1:6774329-6774348 MS.gene45497:CDS 50.0%
CATCTAACACCAAGCCTGGT+AGG + chr2.1:6776717-6776736 MS.gene45497:CDS 50.0%
GAACACCGAACCACCAAACT+TGG - chr2.1:6776455-6776474 None:intergenic 50.0%
GATGAAGGGGACGTGTTGTA+AGG + chr2.1:6776172-6776191 MS.gene45497:intron 50.0%
GGTCGATGTGGTCATTGACA+TGG + chr2.1:6774350-6774369 MS.gene45497:CDS 50.0%
GTCAATGACCACATCGACCT+TGG - chr2.1:6774349-6774368 None:intergenic 50.0%
TCTTCATCCACGGAAGTGAC+GGG - chr2.1:6776506-6776525 None:intergenic 50.0%
TGAGAGGGCCTTGATTCCAA+TGG - chr2.1:6776371-6776390 None:intergenic 50.0%
TGTCACTGCACCTCCAAATG+TGG - chr2.1:6775760-6775779 None:intergenic 50.0%
TGTTGGAACAGAATACGGGG+TGG + chr2.1:6775406-6775425 MS.gene45497:CDS 50.0%
! ACATTTGGAGGTGCAGTGAC+AGG + chr2.1:6775759-6775778 MS.gene45497:CDS 50.0%
! ATGGGATTCCTGCCCTTTTC+AGG - chr2.1:6775180-6775199 None:intergenic 50.0%
! ATTTGGAGGTGCAGTGACAG+GGG + chr2.1:6775761-6775780 MS.gene45497:CDS 50.0%
! CATTTGGAGGTGCAGTGACA+GGG + chr2.1:6775760-6775779 MS.gene45497:CDS 50.0%
! CTTAGTCTTCCTACCAGGCT+TGG - chr2.1:6776729-6776748 None:intergenic 50.0%
!!! TTTTAAGGCTGGCTGCTGAG+TGG - chr2.1:6776580-6776599 None:intergenic 50.0%
ATCCTCTGCAAGTGCCCTTG+TGG - chr2.1:6774520-6774539 None:intergenic 55.0%
CGCAATTGCAGCCCCTGTAA+TGG - chr2.1:6775838-6775857 None:intergenic 55.0%
GAACAGAATACGGGGTGGAG+AGG + chr2.1:6775411-6775430 MS.gene45497:CDS 55.0%
GAGGATGCTTGCTTAGAGCC+AGG + chr2.1:6776323-6776342 MS.gene45497:intron 55.0%
GGGGTGTGATCCAAGTTTGG+TGG + chr2.1:6776442-6776461 MS.gene45497:CDS 55.0%
GTCTTCTCCCAAGGTCGATG+TGG + chr2.1:6774338-6774357 MS.gene45497:CDS 55.0%
! GAGGGGGTGTGATCCAAGTT+TGG + chr2.1:6776439-6776458 MS.gene45497:CDS 55.0%
! GGTGCATCTTGCTCTAGACC+TGG - chr2.1:6776344-6776363 None:intergenic 55.0%
! TTACCCAGTCTCTGGTGCCA+CGG - chr2.1:6775439-6775458 None:intergenic 55.0%
GATCCGTGGCACCAGAGACT+GGG + chr2.1:6775433-6775452 MS.gene45497:CDS 60.0%
GCTGGCTGCTGAGTGGTTAC+TGG - chr2.1:6776573-6776592 None:intergenic 60.0%
GTTGGACTCTGTCTCGGGGA+GGG + chr2.1:6776421-6776440 MS.gene45497:CDS 60.0%
TGTTGGACTCTGTCTCGGGG+AGG + chr2.1:6776420-6776439 MS.gene45497:CDS 60.0%
TTGGACTCTGTCTCGGGGAG+GGG + chr2.1:6776422-6776441 MS.gene45497:CDS 60.0%
!! AGCCACAAGGGCACTTGCAG+AGG + chr2.1:6774515-6774534 MS.gene45497:CDS 60.0%
AGGTGCAGTGACAGGGGCTC+TGG + chr2.1:6775767-6775786 MS.gene45497:CDS 65.0%
GGATCCGTGGCACCAGAGAC+TGG + chr2.1:6775432-6775451 MS.gene45497:CDS 65.0%
GGCTGTTCCCGTCACTTCCG+TGG + chr2.1:6776496-6776515 MS.gene45497:CDS 65.0%
TACGGGGTGGAGAGGATCCG+TGG + chr2.1:6775419-6775438 MS.gene45497:CDS 65.0%
TGGACTCTGTCTCGGGGAGG+GGG + chr2.1:6776423-6776442 MS.gene45497:CDS 65.0%


Chromosome Type Strat End Strand Name
chr2.1 gene 6774309 6776865 6774309 ID=MS.gene45497
chr2.1 mRNA 6774309 6776865 6774309 ID=MS.gene45497.t1;Parent=MS.gene45497
chr2.1 exon 6774309 6774422 6774309 ID=MS.gene45497.t1.exon1;Parent=MS.gene45497.t1
chr2.1 CDS 6774309 6774422 6774309 ID=cds.MS.gene45497.t1;Parent=MS.gene45497.t1
chr2.1 exon 6774510 6774561 6774510 ID=MS.gene45497.t1.exon2;Parent=MS.gene45497.t1
chr2.1 CDS 6774510 6774561 6774510 ID=cds.MS.gene45497.t1;Parent=MS.gene45497.t1
chr2.1 exon 6774734 6774765 6774734 ID=MS.gene45497.t1.exon3;Parent=MS.gene45497.t1
chr2.1 CDS 6774734 6774765 6774734 ID=cds.MS.gene45497.t1;Parent=MS.gene45497.t1
chr2.1 exon 6775263 6775296 6775263 ID=MS.gene45497.t1.exon4;Parent=MS.gene45497.t1
chr2.1 CDS 6775263 6775296 6775263 ID=cds.MS.gene45497.t1;Parent=MS.gene45497.t1
chr2.1 exon 6775387 6775454 6775387 ID=MS.gene45497.t1.exon5;Parent=MS.gene45497.t1
chr2.1 CDS 6775387 6775454 6775387 ID=cds.MS.gene45497.t1;Parent=MS.gene45497.t1
chr2.1 exon 6775750 6775885 6775750 ID=MS.gene45497.t1.exon6;Parent=MS.gene45497.t1
chr2.1 CDS 6775750 6775885 6775750 ID=cds.MS.gene45497.t1;Parent=MS.gene45497.t1
chr2.1 exon 6776324 6776865 6776324 ID=MS.gene45497.t1.exon7;Parent=MS.gene45497.t1
chr2.1 CDS 6776324 6776865 6776324 ID=cds.MS.gene45497.t1;Parent=MS.gene45497.t1
Gene Sequence

>MS.gene45497

ATGCCTCATAGCAGCATTTCAGGATCAGTGTCTTCTCCCAAGGTCGATGTGGTCATTGACATGGGCAATCCACTTCTCAACCTCACTGTTGATGGTTTCTTGAAGATTGGAGCTGTAATTTCTCAACTTCTTCAATTGTTTCTCTTTCTTCTTTATCTTAAACAATTCGAACCAACTATTTATTTTTTATTTTTTTTTCAGGTTGCAGCCACAAGGGCACTTGCAGAGGATACCTATCATATTGCTCGAAAGGGTAAGTATTTCTCCACTTTTGCTATAACCCTTTTGTTAAAATCTCCAAAAGATGAAAACTTTGATTCGGGTTACTATAGACCAAAATCAATATATTATTATTTTCATTTGTGAAGATCTTTGTTTGACTAACTAAATATCATGTCTGATACTTCTAAACATGTCTGTTCCAGGAAGTGTCTCAAGTAGTGATTTTGAGAAAACTGTAAGTTCATTATTTAACATTGTTATCAACTTATCATGATTGATTCATTTGGGGTGGCCTTTACTCCTAAAATATAATTTTTAATATTTTCAGTTGTATAAGCTCCAATTATAAGTAATTTATATATTGGTATGTATGCATCTTTGAGGCAATAATGTGATTTGAACTTGGGAGGTTTAGTCCTAGAAAAGAATAATGACAGGAAAGCCCCTAAATTCGGATTCTCTCATTTCACGAAATCACACATTTGAAGCAATAATGTAGTTTGAGCTGTTTGATCAAAATCGGACAATCCATGCTTTAACTTTGATAAATAGGTCAAATTACAAAGCTGATACACGAGCCATCTGATCTTGATATGTCAGTCTATATCCGCAAGTGTGTGATTACGTGAATGCTGAGAATCTAAATCCTGAAAAGGGCAGGAATCCCATTCAATAGACATTTTGTCTACCATGATATGCTTTGTTCCTTGTTAATGAGAGGTCTTGTTGCAGCTGAAGAAGACATTTAAAGAAGGTGCATACTGGGGTAAGACTTGTGTTGCATTATGTAATATGACAAGTTCAATTAGTAAACACATTAGCTCTATTTAATGTATTTTCCTTTCTACGCTTACAGGAGCTATAGCTGGTGTATATGTTGGAACAGAATACGGGGTGGAGAGGATCCGTGGCACCAGAGACTGGGTAATTCTCTCAATAGGGTAGTTGAAAACACTTAAAATAGACTTGGTGTTATTGTACATTCTATGCTAGAGAGAGCACTTTATTTCTGGATTTGTCTTCCATATAATGACATCTAATCTCTTAAATTTCAAGCTGTTTTTAACAAGCATCATGTTTGAAAATGAATCTGTAAAAGTTAGATGTCAAATGTGTTAACTTTGTTTTAGGCAGTGAGCCATGCCTTCTATTGTTCAATTTAAGTTAGATTTTTCAGTTGAGATTCTTACCGTTGTTCCCTTGGTCTTTTGATGTACAGAAAAATGCCACATTTGGAGGTGCAGTGACAGGGGCTCTGGTATCTGCAGCCAACAATGAAAAGGCAGACAAGATTGTAGTAGGTGCCATTACAGGGGCTGCAATTGCGACTGCTGCAGCATTCATAAATGGCTGGAGTTTCTAATGGATGATTTAAATGTTTAATGCTATTATTCCATGTTTGAAAAGGGGTTGCACATTTGGTCTCTTTTGTCTGTCTTACCGGTTATGTAATAATTTTGATGGAATTACTGATGAGACGTCATATTTCAGCTTTCTGTCAATTCTTGGTCTTTGGATAAATGAGTTTTACTTTTAGTATAAAGAAACCTGGGCATGAACAGGTTTTCAAATTTAAACATTAGCAGCCAGGTGAATAAAGTTTAACGTTTTCATAAATATTATTGCGGTTCTAGATCATGAGATGAAGGGGACGTGTTGTAAGGTAGGAATGTCTCCCTGTTGAAATCATGATATATATCAATCTATCTAATTGCCGTTGCTTTAAAAAATACTGAACTAACTAAAGCAGTAAATTTAGATTTTATAATACATTTTCTGTGTATATCCTAAAGAGGATGCTTGCTTAGAGCCAGGTCTAGAGCAAGATGCACCTATTCCATTGGAATCAAGGCCCTCTCAGCCTCTACCTATAGTTTCTAACAAGCAAATGTTGGACTCTGTCTCGGGGAGGGGGTGTGATCCAAGTTTGGTGGTTCGGTGTTCTAGCACCCCAGTTTTGTCTTTGGCTGTTCCCGTCACTTCCGTGGATGAAGATTTGAGTCTGGGCAAAAGAGTTATAGGTGGAAAGTCTGGTGACACCAGTAACCACTCAGCAGCCAGCCTTAAAAGTGTGCAGATAATGAAGGAATTTTGGGGTGATGTTGATGACGACGACTCTGCCTCAGACATTAACACTGATATGGAGCATGCAGCTGACAGATACTTAGCCAAACATACTGACATAGCATCTAACACCAAGCCTGGTAGGAAGACTAAGAAGCAGAAAAGCCCTAAGACTCACATTGGCAAAGGTTCTGATGGCATCACAAGTAGTGAGCACATCCAAACTCGTTCCAAAAAGGGTGTAATCAAAAGCAATCCCAAATATTTGTGA
Protein sequence

>MS.gene45497.t1

MPHSSISGSVSSPKVDVVIDMGNPLLNLTVDGFLKIGAVAATRALAEDTYHIARKGSVSSSDFEKTLKKTFKEGAYWGAIAGVYVGTEYGVERIRGTRDWKNATFGGAVTGALVSAANNEKADKIVVGAITGAAIATAAAFINGWKDACLEPGLEQDAPIPLESRPSQPLPIVSNKQMLDSVSGRGCDPSLVVRCSSTPVLSLAVPVTSVDEDLSLGKRVIGGKSGDTSNHSAASLKSVQIMKEFWGDVDDDDSASDINTDMEHAADRYLAKHTDIASNTKPGRKTKKQKSPKTHIGKGSDGITSSEHIQTRSKKGVIKSNPKYL