Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene45662.t1 | RHN38880.1 | 87.3 | 71 | 9 | 0 | 1 | 71 | 32 | 102 | 4.00E-24 | 120.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene45662.t1 | G7ZWR4 | 87.3 | 71 | 9 | 0 | 1 | 71 | 32 | 102 | 2.9e-24 | 120.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049378 | MS.gene45662 | 0.806035 | 1.00E-49 | -1.69E-46 |
| MS.gene050807 | MS.gene45662 | 0.814621 | 1.40E-51 | -1.69E-46 |
| MS.gene052176 | MS.gene45662 | 0.814396 | 1.57E-51 | -1.69E-46 |
| MS.gene052302 | MS.gene45662 | 0.826811 | 2.21E-54 | -1.69E-46 |
| MS.gene052376 | MS.gene45662 | 0.83805 | 3.62E-57 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene45662.t1 | MTR_8g010440 | 87.324 | 71 | 9 | 0 | 1 | 71 | 32 | 102 | 1.29e-34 | 123 |
| MS.gene45662.t1 | MTR_6g452490 | 84.507 | 71 | 11 | 0 | 1 | 71 | 32 | 102 | 1.55e-33 | 120 |
| MS.gene45662.t1 | MTR_8g011300 | 83.099 | 71 | 12 | 0 | 1 | 71 | 32 | 102 | 1.23e-32 | 117 |
| MS.gene45662.t1 | MTR_0065s0100 | 76.389 | 72 | 16 | 1 | 1 | 71 | 32 | 103 | 2.25e-29 | 108 |
| MS.gene45662.t1 | MTR_8g011550 | 77.778 | 72 | 15 | 1 | 1 | 71 | 32 | 103 | 6.50e-29 | 107 |
| MS.gene45662.t1 | MTR_8g027540 | 75.000 | 72 | 17 | 1 | 1 | 71 | 32 | 103 | 2.85e-28 | 105 |
| MS.gene45662.t1 | MTR_1g030770 | 75.714 | 70 | 17 | 0 | 2 | 71 | 33 | 102 | 2.33e-27 | 102 |
| MS.gene45662.t1 | MTR_7g013560 | 68.056 | 72 | 22 | 1 | 1 | 71 | 32 | 103 | 4.57e-26 | 99.4 |
| MS.gene45662.t1 | MTR_8g011320 | 77.465 | 71 | 5 | 1 | 1 | 71 | 32 | 91 | 1.85e-25 | 97.4 |
| MS.gene45662.t1 | MTR_8g011180 | 71.429 | 70 | 18 | 2 | 3 | 71 | 34 | 102 | 7.38e-25 | 95.9 |
| MS.gene45662.t1 | MTR_8g011170 | 69.014 | 71 | 22 | 0 | 1 | 71 | 32 | 102 | 1.11e-24 | 95.5 |
| MS.gene45662.t1 | MTR_8g037220 | 65.714 | 70 | 24 | 0 | 1 | 70 | 811 | 880 | 1.32e-24 | 95.1 |
| MS.gene45662.t1 | MTR_8g037220 | 65.714 | 70 | 24 | 0 | 1 | 70 | 1032 | 1101 | 1.43e-24 | 95.1 |
| MS.gene45662.t1 | MTR_8g011850 | 64.286 | 70 | 20 | 1 | 1 | 70 | 1133 | 1197 | 1.03e-20 | 84.0 |
| MS.gene45662.t1 | MTR_3g063360 | 66.071 | 56 | 19 | 0 | 2 | 57 | 33 | 88 | 6.69e-19 | 73.9 |
| MS.gene45662.t1 | MTR_4g025370 | 66.071 | 56 | 19 | 0 | 2 | 57 | 44 | 99 | 7.40e-19 | 74.3 |
| MS.gene45662.t1 | MTR_4g025370 | 66.071 | 56 | 19 | 0 | 2 | 57 | 33 | 88 | 9.48e-19 | 73.6 |
| MS.gene45662.t1 | MTR_3g015500 | 48.529 | 68 | 33 | 2 | 2 | 69 | 66 | 131 | 2.35e-12 | 60.5 |
| MS.gene45662.t1 | MTR_4g050790 | 48.529 | 68 | 33 | 2 | 2 | 69 | 28 | 93 | 2.54e-12 | 60.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 9 sgRNAs with CRISPR-Local
Find 9 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTATGGCGTGATGGATGAAT+TGG | 0.365156 | 8.3:-78047472 | None:intergenic |
| CTTGTTTCTCTTCAGCATCA+AGG | 0.429709 | 8.3:+78047409 | None:intergenic |
| TTGAAGATATGATACGCAAA+AGG | 0.433124 | 8.3:-78047302 | MS.gene45662:CDS |
| ATCAAGGAGGACAACTTTGA+TGG | 0.436069 | 8.3:+78047425 | None:intergenic |
| TCATCTATCAAATCATCAGC+AGG | 0.529654 | 8.3:+78047333 | None:intergenic |
| TGAAGATATGATACGCAAAA+GGG | 0.550820 | 8.3:-78047301 | MS.gene45662:CDS |
| TGATAAGAACAAAGTGACAC+AGG | 0.647114 | 8.3:-78047271 | None:intergenic |
| GTTTCTCTTCAGCATCAAGG+AGG | 0.673420 | 8.3:+78047412 | None:intergenic |
| GCGTGATGGATGAATTGGAA+AGG | 0.681103 | 8.3:-78047467 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAAGTCATTCTGTAAAAAAC+TGG | - | chr8.3:78047351-78047370 | MS.gene45662:CDS | 25.0% |
| ! | AAGTCATTCTGTAAAAAACT+GGG | - | chr8.3:78047352-78047371 | MS.gene45662:CDS | 25.0% |
| TGAAGATATGATACGCAAAA+GGG | - | chr8.3:78047433-78047452 | MS.gene45662:CDS | 30.0% | |
| TTGAAGATATGATACGCAAA+AGG | - | chr8.3:78047432-78047451 | MS.gene45662:CDS | 30.0% | |
| TCATCTATCAAATCATCAGC+AGG | + | chr8.3:78047404-78047423 | None:intergenic | 35.0% | |
| ! | CTGTAAAAAACTGGGTTAGA+AGG | - | chr8.3:78047360-78047379 | MS.gene45662:CDS | 35.0% |
| ATCAAGGAGGACAACTTTGA+TGG | + | chr8.3:78047312-78047331 | None:intergenic | 40.0% | |
| ! | CTTGTTTCTCTTCAGCATCA+AGG | + | chr8.3:78047328-78047347 | None:intergenic | 40.0% |
| GTTTCTCTTCAGCATCAAGG+AGG | + | chr8.3:78047325-78047344 | None:intergenic | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 78047272 | 78047484 | 78047272 | ID=MS.gene45662 |
| chr8.3 | mRNA | 78047272 | 78047484 | 78047272 | ID=MS.gene45662.t1;Parent=MS.gene45662 |
| chr8.3 | exon | 78047272 | 78047484 | 78047272 | ID=MS.gene45662.t1.exon1;Parent=MS.gene45662.t1 |
| chr8.3 | CDS | 78047272 | 78047484 | 78047272 | ID=cds.MS.gene45662.t1;Parent=MS.gene45662.t1 |
| Gene Sequence |
| Protein sequence |
