AlfalfaGEDB | MS.gene45964

M. sativa cultivar XinJiangDaYe / MS.gene45964

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene45964.t1	XP_024625231.1	89.4	104	11	0	38	141	970	1073	1.10E-46	196.1

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene45964.t1	Q9C6A8	37.9	87	49	2	36	122	872	953	1.2e-07	57.8

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene45964.t1	A0A396GZ39	89.4	104	11	0	38	141	1025	1128	8.2e-47	196.1

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene053641	MS.gene45964	0.803285	3.75E-49	-1.69E-46
MS.gene05415	MS.gene45964	0.800157	1.64E-48	-1.69E-46
MS.gene054291	MS.gene45964	-0.802247	6.14E-49	-1.69E-46
MS.gene05435	MS.gene45964	0.815194	1.05E-51	-1.69E-46
MS.gene058378	MS.gene45964	0.80103	1.09E-48	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene45964.t1	MTR_7g066590	85.816	141	20	0	1	141	663	803	3.83e-81	254
MS.gene45964.t1	MTR_7g066620	78.125	96	21	0	1	96	726	821	1.77e-46	160
MS.gene45964.t1	MTR_4g016820	47.107	121	60	2	2	122	152	268	1.67e-29	108
MS.gene45964.t1	MTR_4g016850	47.107	121	60	2	2	122	801	917	1.97e-27	106
MS.gene45964.t1	MTR_4g016910	46.281	121	61	2	2	122	573	689	1.23e-26	104
MS.gene45964.t1	MTR_4g015930	47.107	121	60	2	2	122	676	792	1.39e-26	103
MS.gene45964.t1	MTR_4g015930	47.107	121	60	2	2	122	678	794	1.41e-26	103
MS.gene45964.t1	MTR_4g015930	47.107	121	60	2	2	122	678	794	1.43e-26	103
MS.gene45964.t1	MTR_4g015930	47.107	121	60	2	2	122	896	1012	1.59e-26	103
MS.gene45964.t1	MTR_4g015930	47.107	121	60	2	2	122	588	704	1.65e-26	103
MS.gene45964.t1	MTR_4g015930	47.107	121	60	2	2	122	896	1012	1.67e-26	103
MS.gene45964.t1	MTR_4g015930	47.107	121	60	2	2	122	588	704	1.73e-26	103
MS.gene45964.t1	MTR_4g015930	47.107	121	60	2	2	122	896	1012	1.78e-26	103
MS.gene45964.t1	MTR_4g016800	44.715	123	64	2	2	124	676	794	2.48e-25	100
MS.gene45964.t1	MTR_4g015960	45.370	108	55	2	2	109	492	595	2.60e-20	86.3
MS.gene45964.t1	MTR_8g077105	36.923	130	73	3	2	130	624	745	5.14e-20	85.5
MS.gene45964.t1	MTR_2g016200	36.154	130	74	3	2	130	756	877	2.37e-19	83.6
MS.gene45964.t1	MTR_4g009930	36.154	130	74	3	2	130	190	311	6.66e-19	81.3
MS.gene45964.t1	MTR_2g017805	33.600	125	74	4	2	122	264	383	4.55e-16	73.9
MS.gene45964.t1	MTR_2g017420	35.000	140	88	3	2	140	852	989	7.58e-16	73.6
MS.gene45964.t1	MTR_4g040360	38.017	121	65	4	2	122	903	1013	2.00e-15	72.4
MS.gene45964.t1	MTR_2g017480	33.571	140	76	5	2	137	838	964	1.10e-14	70.1
MS.gene45964.t1	MTR_2g017495	31.944	144	88	5	2	140	655	793	1.70e-13	66.6
MS.gene45964.t1	MTR_6g038760	32.374	139	83	5	2	135	278	410	7.28e-13	64.7
MS.gene45964.t1	MTR_6g039110	33.333	129	81	4	2	130	956	1079	9.38e-13	64.7
MS.gene45964.t1	MTR_7g009580	42.353	85	43	2	2	86	792	870	1.24e-12	64.3
MS.gene45964.t1	MTR_4g016780	44.318	88	45	2	2	89	563	646	1.51e-12	63.9
MS.gene45964.t1	MTR_5g080000	47.826	69	35	1	1	69	800	867	2.21e-12	63.5
MS.gene45964.t1	MTR_5g079980	47.826	69	35	1	1	69	894	961	2.36e-12	63.5
MS.gene45964.t1	MTR_6g038790	34.615	130	77	6	2	130	490	612	5.16e-12	62.4
MS.gene45964.t1	MTR_6g038790	34.615	130	77	6	2	130	500	622	5.92e-12	62.4
MS.gene45964.t1	MTR_6g016200	34.400	125	70	4	2	121	296	413	6.04e-12	62.0
MS.gene45964.t1	MTR_0087s0070	34.167	120	77	2	2	121	579	696	6.65e-12	62.0
MS.gene45964.t1	MTR_0087s0020	31.667	120	79	2	2	121	775	891	7.89e-12	62.0
MS.gene45964.t1	MTR_6g038980	33.846	130	79	5	2	130	671	794	1.60e-11	60.8
MS.gene45964.t1	MTR_7g007820	54.237	59	26	1	2	60	821	878	1.65e-11	60.8
MS.gene45964.t1	MTR_5g094820	45.833	72	37	2	1	72	740	809	1.86e-11	60.8
MS.gene45964.t1	MTR_7g009520	50.000	64	30	2	2	64	186	248	1.97e-11	60.5
MS.gene45964.t1	MTR_6g016140	34.426	122	75	4	2	121	801	919	2.10e-11	60.8
MS.gene45964.t1	MTR_5g085970	28.986	138	92	3	3	140	854	985	2.72e-11	60.5
MS.gene45964.t1	MTR_7g009790	44.186	86	45	2	2	87	741	823	3.31e-11	60.1
MS.gene45964.t1	MTR_5g085920	28.986	138	93	2	3	140	1006	1138	3.70e-11	60.1
MS.gene45964.t1	MTR_6g039180	33.065	124	72	4	2	122	1054	1169	4.32e-11	59.7
MS.gene45964.t1	MTR_5g096340	46.377	69	36	1	1	69	905	972	4.58e-11	59.7
MS.gene45964.t1	MTR_6g037750	35.246	122	72	5	2	122	1073	1188	5.04e-11	59.7
MS.gene45964.t1	MTR_5g095420	51.667	60	28	1	1	60	727	785	6.03e-11	59.3
MS.gene45964.t1	MTR_7g009510	50.847	59	28	1	2	60	798	855	9.77e-11	58.9

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene45964.t1	AT1G74190	42.975	121	63	3	2	122	839	953	9.24e-24	96.3
MS.gene45964.t1	AT2G25470	39.344	122	71	2	1	122	848	966	6.01e-22	90.9
MS.gene45964.t1	AT2G25470	39.344	122	71	2	1	122	805	923	6.04e-22	90.9
MS.gene45964.t1	AT5G49290	39.344	122	71	2	1	122	750	868	1.02e-21	90.1
MS.gene45964.t1	AT5G49290	39.344	122	71	2	1	122	778	896	1.03e-21	90.1
MS.gene45964.t1	AT5G49290	39.344	122	71	2	1	122	817	935	1.03e-21	90.1
MS.gene45964.t1	AT5G49290	39.344	122	71	2	1	122	803	921	1.11e-21	90.1
MS.gene45964.t1	AT5G49290	39.344	122	71	2	1	122	803	921	1.11e-21	90.1
MS.gene45964.t1	AT5G49290	39.344	122	71	2	1	122	803	921	1.11e-21	90.1
MS.gene45964.t1	AT5G49290	39.344	122	71	2	1	122	560	678	1.15e-21	90.1
MS.gene45964.t1	AT5G49290	39.344	122	71	2	1	122	775	893	1.18e-21	90.1
MS.gene45964.t1	AT1G74170	40.496	121	66	3	2	122	907	1021	4.83e-18	79.7
MS.gene45964.t1	AT1G74170	40.496	121	66	3	2	122	839	953	4.99e-18	79.7
MS.gene45964.t1	AT1G54475	37.705	122	73	2	1	122	79	197	2.65e-17	75.1
MS.gene45964.t1	AT1G58190	33.607	122	78	2	1	122	901	1019	8.61e-17	76.3
MS.gene45964.t1	AT1G58190	33.607	122	78	2	1	122	812	930	9.05e-17	76.3
MS.gene45964.t1	AT1G58190	33.607	122	78	2	1	122	639	757	9.38e-17	76.3
MS.gene45964.t1	AT1G07390	35.821	134	82	3	2	132	909	1041	2.00e-16	75.1
MS.gene45964.t1	AT1G07390	35.821	134	82	3	2	132	938	1070	2.08e-16	75.1
MS.gene45964.t1	AT1G07390	37.302	126	75	3	2	124	944	1068	2.25e-16	75.1
MS.gene45964.t1	AT1G07390	37.302	126	75	3	2	124	895	1019	2.40e-16	75.1
MS.gene45964.t1	AT1G07390	37.302	126	75	3	2	124	774	898	2.80e-16	74.7
MS.gene45964.t1	AT3G53240	37.705	122	69	3	2	122	798	913	1.36e-15	72.8
MS.gene45964.t1	AT3G53240	37.705	122	69	3	2	122	762	877	1.52e-15	72.8
MS.gene45964.t1	AT3G53240	37.705	122	69	3	2	122	762	877	1.52e-15	72.8
MS.gene45964.t1	AT1G58190	33.065	124	79	2	1	124	805	924	1.58e-14	69.7
MS.gene45964.t1	AT1G74180	34.677	124	78	2	1	122	842	964	2.33e-13	66.2
MS.gene45964.t1	AT1G74180	34.677	124	78	2	1	122	828	950	2.44e-13	66.2

Find 34 sgRNAs with CRISPR-Local

Find 41 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTCTGTACATCAATCCTTAT+TGG	0.175330	7.2:-44717875	MS.gene45964:CDS
AACCATGCCTGCCTCCAATA+AGG	0.380348	7.2:+44717861	None:intergenic
GTGGCACACAACAATTTATC+TGG	0.392683	7.2:-44718134	MS.gene45964:CDS
TATGAATTGCTACTACTTCA+TGG	0.402485	7.2:-44717817	MS.gene45964:CDS
TATCTGGTGCAACACTTGAA+TGG	0.421197	7.2:-44718118	MS.gene45964:CDS
CCAGATGGTTTGAACAATGA+TGG	0.438652	7.2:-44717984	MS.gene45964:CDS
GAGGGTAATCAATTTCTTTG+TGG	0.441786	7.2:-44718059	MS.gene45964:CDS
CCATCATTGTTCAAACCATC+TGG	0.442758	7.2:+44717984	None:intergenic
GAAGCAGGTGGTTGTCCACT+TGG	0.456669	7.2:+44718011	None:intergenic
GTGCCACACTGAATACTTCA+AGG	0.505071	7.2:+44718150	None:intergenic
ATCCTTATTGGAGGCAGGCA+TGG	0.505721	7.2:-44717863	MS.gene45964:CDS
ATGATGGAGATAATGATAGT+TGG	0.507461	7.2:-44717968	MS.gene45964:CDS
TGATGGAGATAATGATAGTT+GGG	0.508179	7.2:-44717967	MS.gene45964:CDS
CATCAATCCTTATTGGAGGC+AGG	0.526445	7.2:-44717868	MS.gene45964:CDS
GGCATACACATCAGTAGTGT+TGG	0.526931	7.2:-44717910	MS.gene45964:CDS
TGTACATCAATCCTTATTGG+AGG	0.527064	7.2:-44717872	MS.gene45964:CDS
AAACCATCTGGTAATGAAGC+AGG	0.529675	7.2:+44717996	None:intergenic
CCACCTGCTTCATTACCAGA+TGG	0.579365	7.2:-44717999	MS.gene45964:CDS
CTTTCTTTCAACATGTTGAG+TGG	0.602694	7.2:-44718206	None:intergenic
AGAATTCCTCCTCGGCTAAG+TGG	0.603592	7.2:-44718182	MS.gene45964:CDS
TTTCCGTGTGAGCTTTGTTG+TGG	0.604918	7.2:-44717931	MS.gene45964:CDS
GTCAATCCACTTAGCCGAGG+AGG	0.617111	7.2:+44718176	None:intergenic
TGCCACACTGAATACTTCAA+GGG	0.624094	7.2:+44718151	None:intergenic
ATGCCACAACAAAGCTCACA+CGG	0.628884	7.2:+44717928	None:intergenic
TTTGATGAAAGCAGCTACGA+GGG	0.631447	7.2:-44718077	MS.gene45964:CDS
GGTGCAACACTTGAATGGAA+AGG	0.643614	7.2:-44718113	MS.gene45964:CDS
TGAGTGGCAGAATTCCTCCT+CGG	0.653914	7.2:-44718190	MS.gene45964:CDS
GAGGTCAATCCACTTAGCCG+AGG	0.675215	7.2:+44718173	None:intergenic
CACACTGAATACTTCAAGGG+AGG	0.679540	7.2:+44718154	None:intergenic
CCATCTGGTAATGAAGCAGG+TGG	0.687214	7.2:+44717999	None:intergenic
ATTTGATGAAAGCAGCTACG+AGG	0.696683	7.2:-44718078	MS.gene45964:CDS
TGGAGATAATGATAGTTGGG+TGG	0.699675	7.2:-44717964	MS.gene45964:CDS
CTCCCTTGAAGTATTCAGTG+TGG	0.704965	7.2:-44718153	MS.gene45964:CDS
GAATTGCTACTACTTCATGG+AGG	0.765267	7.2:-44717814	MS.gene45964:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	TATGAATTGCTACTACTTCA+TGG	-	chr7.2:44718168-44718187	MS.gene45964:CDS	30.0%
	TGATGGAGATAATGATAGTT+GGG	-	chr7.2:44718018-44718037	MS.gene45964:CDS	30.0%
	TTCTGTACATCAATCCTTAT+TGG	-	chr7.2:44718110-44718129	MS.gene45964:CDS	30.0%
!	ATGATGGAGATAATGATAGT+TGG	-	chr7.2:44718017-44718036	MS.gene45964:CDS	30.0%
!!	ATGGTTTTACTATATAGGAC+TGG	-	chr7.2:44718141-44718160	MS.gene45964:CDS	30.0%
!!	TTTTACTATATAGGACTGGT+GGG	-	chr7.2:44718145-44718164	MS.gene45964:CDS	30.0%
	GAGGGTAATCAATTTCTTTG+TGG	-	chr7.2:44717926-44717945	MS.gene45964:CDS	35.0%
	TGTACATCAATCCTTATTGG+AGG	-	chr7.2:44718113-44718132	MS.gene45964:CDS	35.0%
!	GTTTTACTATATAGGACTGG+TGG	-	chr7.2:44718144-44718163	MS.gene45964:CDS	35.0%
!!	CAGGCATGGTTTTACTATAT+AGG	-	chr7.2:44718136-44718155	MS.gene45964:CDS	35.0%
	ATTTGATGAAAGCAGCTACG+AGG	-	chr7.2:44717907-44717926	MS.gene45964:CDS	40.0%
	CCAAAAAGTTGCAATCCAAG+TGG	-	chr7.2:44717959-44717978	MS.gene45964:CDS	40.0%
	CCAGATGGTTTGAACAATGA+TGG	-	chr7.2:44718001-44718020	MS.gene45964:CDS	40.0%
	CCATCATTGTTCAAACCATC+TGG	+	chr7.2:44718004-44718023	None:intergenic	40.0%
	GAATTGCTACTACTTCATGG+AGG	-	chr7.2:44718171-44718190	MS.gene45964:CDS	40.0%
	GTGGCACACAACAATTTATC+TGG	-	chr7.2:44717851-44717870	MS.gene45964:CDS	40.0%
	TATCTGGTGCAACACTTGAA+TGG	-	chr7.2:44717867-44717886	MS.gene45964:CDS	40.0%
	TGCCACACTGAATACTTCAA+GGG	+	chr7.2:44717837-44717856	None:intergenic	40.0%
	TGGAGATAATGATAGTTGGG+TGG	-	chr7.2:44718021-44718040	MS.gene45964:CDS	40.0%
	TTTGATGAAAGCAGCTACGA+GGG	-	chr7.2:44717908-44717927	MS.gene45964:CDS	40.0%
!	AAACCATCTGGTAATGAAGC+AGG	+	chr7.2:44717992-44718011	None:intergenic	40.0%
!	CCACTTGGATTGCAACTTTT+TGG	+	chr7.2:44717962-44717981	None:intergenic	40.0%
	ATGCCACAACAAAGCTCACA+CGG	+	chr7.2:44718060-44718079	None:intergenic	45.0%
	CACACTGAATACTTCAAGGG+AGG	+	chr7.2:44717834-44717853	None:intergenic	45.0%
	CATCAATCCTTATTGGAGGC+AGG	-	chr7.2:44718117-44718136	MS.gene45964:CDS	45.0%
	GGCATACACATCAGTAGTGT+TGG	-	chr7.2:44718075-44718094	MS.gene45964:CDS	45.0%
	GGTGCAACACTTGAATGGAA+AGG	-	chr7.2:44717872-44717891	MS.gene45964:CDS	45.0%
	GTGCCACACTGAATACTTCA+AGG	+	chr7.2:44717838-44717857	None:intergenic	45.0%
!	CTCCCTTGAAGTATTCAGTG+TGG	-	chr7.2:44717832-44717851	MS.gene45964:CDS	45.0%
!!	GGATTGCAACTTTTTGGCAG+TGG	+	chr7.2:44717956-44717975	None:intergenic	45.0%
!!	TTGCAACTTTTTGGCAGTGG+TGG	+	chr7.2:44717953-44717972	None:intergenic	45.0%
!!	TTTCCGTGTGAGCTTTGTTG+TGG	-	chr7.2:44718054-44718073	MS.gene45964:CDS	45.0%
	AACCATGCCTGCCTCCAATA+AGG	+	chr7.2:44718127-44718146	None:intergenic	50.0%
	AGAATTCCTCCTCGGCTAAG+TGG	-	chr7.2:44717803-44717822	MS.gene45964:CDS	50.0%
	ATCCTTATTGGAGGCAGGCA+TGG	-	chr7.2:44718122-44718141	MS.gene45964:CDS	50.0%
	CCACCTGCTTCATTACCAGA+TGG	-	chr7.2:44717986-44718005	MS.gene45964:CDS	50.0%
	TGAGTGGCAGAATTCCTCCT+CGG	-	chr7.2:44717795-44717814	MS.gene45964:CDS	50.0%
!	CCATCTGGTAATGAAGCAGG+TGG	+	chr7.2:44717989-44718008	None:intergenic	50.0%
	GAAGCAGGTGGTTGTCCACT+TGG	+	chr7.2:44717977-44717996	None:intergenic	55.0%
	GAGGTCAATCCACTTAGCCG+AGG	+	chr7.2:44717815-44717834	None:intergenic	55.0%
	GTCAATCCACTTAGCCGAGG+AGG	+	chr7.2:44717812-44717831	None:intergenic	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr7.2	gene	44717791	44718216	44717791	ID=MS.gene45964
chr7.2	mRNA	44717791	44718216	44717791	ID=MS.gene45964.t1;Parent=MS.gene45964
chr7.2	exon	44717791	44718216	44717791	ID=MS.gene45964.t1.exon1;Parent=MS.gene45964.t1
chr7.2	CDS	44717791	44718216	44717791	ID=cds.MS.gene45964.t1;Parent=MS.gene45964.t1

Gene Sequence

>MS.gene45964

ATGTTGAGTGGCAGAATTCCTCCTCGGCTAAGTGGATTGACCTCCCTTGAAGTATTCAGTGTGGCACACAACAATTTATCTGGTGCAACACTTGAATGGAAAGGACAATTTAGTACATTTGATGAAAGCAGCTACGAGGGTAATCAATTTCTTTGTGGACCACCACTGCCAAAAAGTTGCAATCCAAGTGGACAACCACCTGCTTCATTACCAGATGGTTTGAACAATGATGGAGATAATGATAGTTGGGTGGACATGTATGTTTTCCGTGTGAGCTTTGTTGTGGCATACACATCAGTAGTGTTGGTAATTGCAACTGTTCTGTACATCAATCCTTATTGGAGGCAGGCATGGTTTTACTATATAGGACTGGTGGGTATGAATTGCTACTACTTCATGGAGGACAACTTGTACAAGTTTTTTTAA

Protein sequence

>MS.gene45964.t1

MLSGRIPPRLSGLTSLEVFSVAHNNLSGATLEWKGQFSTFDESSYEGNQFLCGPPLPKSCNPSGQPPASLPDGLNNDGDNDSWVDMYVFRVSFVVAYTSVVLVIATVLYINPYWRQAWFYYIGLVGMNCYYFMEDNLYKFF