Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene46067.t1 | XP_003623161.2 | 91.1 | 112 | 10 | 0 | 1 | 112 | 1 | 112 | 2.80E-51 | 211.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene46067.t1 | G7L5L2 | 91.1 | 112 | 10 | 0 | 1 | 112 | 1 | 112 | 2.0e-51 | 211.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene055158 | MS.gene46067 | 0.864294 | 1.35E-64 | -1.69E-46 |
| MS.gene055159 | MS.gene46067 | 0.846942 | 1.58E-59 | -1.69E-46 |
| MS.gene058418 | MS.gene46067 | 0.808109 | 3.64E-50 | -1.69E-46 |
| MS.gene05960 | MS.gene46067 | 0.830705 | 2.53E-55 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene46067.t1 | MTR_7g065160 | 91.071 | 112 | 10 | 0 | 1 | 112 | 1 | 112 | 5.29e-72 | 210 |
| MS.gene46067.t1 | MTR_7g065210 | 87.500 | 112 | 14 | 0 | 1 | 112 | 1 | 112 | 6.42e-69 | 202 |
| MS.gene46067.t1 | MTR_8g070040 | 47.368 | 95 | 48 | 2 | 20 | 113 | 23 | 116 | 1.26e-21 | 83.2 |
| MS.gene46067.t1 | MTR_8g070015 | 45.652 | 92 | 48 | 2 | 20 | 110 | 23 | 113 | 6.14e-19 | 75.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene46067.t1 | AT5G05960 | 61.321 | 106 | 34 | 2 | 7 | 112 | 13 | 111 | 1.42e-39 | 128 |
| MS.gene46067.t1 | AT3G53980 | 63.333 | 90 | 32 | 1 | 23 | 112 | 21 | 109 | 1.46e-39 | 128 |
| MS.gene46067.t1 | AT3G53980 | 63.333 | 90 | 32 | 1 | 23 | 112 | 21 | 109 | 1.46e-39 | 128 |
| MS.gene46067.t1 | AT2G37870 | 45.714 | 105 | 53 | 3 | 11 | 113 | 9 | 111 | 2.98e-23 | 87.0 |
Find 18 sgRNAs with CRISPR-Local
Find 19 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGCTTAAATGCTTCATTATC+TGG | 0.223692 | 7.2:+42675186 | None:intergenic |
| CAGCAAGGTTGCAACGTTTA+GGG | 0.315042 | 7.2:+42675001 | None:intergenic |
| TCAGCAAGGTTGCAACGTTT+AGG | 0.342606 | 7.2:+42675000 | None:intergenic |
| TCAAACGTTGCTAAAATGTC+TGG | 0.431901 | 7.2:-42675051 | MS.gene46067:CDS |
| CTCACAAAGTTGTTGTGCTC+AGG | 0.452580 | 7.2:-42675121 | MS.gene46067:CDS |
| GGGATAGTAACAACAATTTG+AGG | 0.457491 | 7.2:+42675021 | None:intergenic |
| TGTGCTCAGGTTAAGAAACT+TGG | 0.492815 | 7.2:-42675108 | MS.gene46067:CDS |
| GGATAGTAACAACAATTTGA+GGG | 0.498325 | 7.2:+42675022 | None:intergenic |
| GTTGAAAGTGCTGGTGAATG+TGG | 0.539242 | 7.2:-42675222 | MS.gene46067:CDS |
| AACCTTGCTGATCGTCCTGT+CGG | 0.547957 | 7.2:-42674988 | MS.gene46067:CDS |
| ATTCATAGAGTTGAAAGTGC+TGG | 0.554882 | 7.2:-42675231 | MS.gene46067:CDS |
| TCTTGTGCTGCACTTGCACA+AGG | 0.557668 | 7.2:+42675159 | None:intergenic |
| TGTCCGCACTTGTAACCGAC+AGG | 0.588008 | 7.2:+42674973 | None:intergenic |
| ATAACAGCACAAAGACAACT+TGG | 0.604458 | 7.2:+42675078 | None:intergenic |
| AGTGCTGGTGAATGTGGAAG+AGG | 0.608175 | 7.2:-42675216 | MS.gene46067:CDS |
| CAAAGACTACAAAACAAACA+AGG | 0.620950 | 7.2:+42675262 | None:intergenic |
| CGTCCTGTCGGTTACAAGTG+CGG | 0.640263 | 7.2:-42674976 | MS.gene46067:CDS |
| AACCGACAGGACGATCAGCA+AGG | 0.685728 | 7.2:+42674986 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AGCTTAAATGCTTCATTATC+TGG | + | chr7.2:42675071-42675090 | None:intergenic | 30.0% | |
| CAAAGACTACAAAACAAACA+AGG | + | chr7.2:42674995-42675014 | None:intergenic | 30.0% | |
| GGATAGTAACAACAATTTGA+GGG | + | chr7.2:42675235-42675254 | None:intergenic | 30.0% | |
| ATAACAGCACAAAGACAACT+TGG | + | chr7.2:42675179-42675198 | None:intergenic | 35.0% | |
| GGGATAGTAACAACAATTTG+AGG | + | chr7.2:42675236-42675255 | None:intergenic | 35.0% | |
| TCAAACGTTGCTAAAATGTC+TGG | - | chr7.2:42675203-42675222 | MS.gene46067:CDS | 35.0% | |
| ! | ATTCATAGAGTTGAAAGTGC+TGG | - | chr7.2:42675023-42675042 | MS.gene46067:CDS | 35.0% |
| ! | TGTTTTGTAGTCTTTGTTGC+AGG | - | chr7.2:42674999-42675018 | MS.gene46067:CDS | 35.0% |
| TGTGCTCAGGTTAAGAAACT+TGG | - | chr7.2:42675146-42675165 | MS.gene46067:CDS | 40.0% | |
| CAGCAAGGTTGCAACGTTTA+GGG | + | chr7.2:42675256-42675275 | None:intergenic | 45.0% | |
| CTCACAAAGTTGTTGTGCTC+AGG | - | chr7.2:42675133-42675152 | MS.gene46067:CDS | 45.0% | |
| TCAGCAAGGTTGCAACGTTT+AGG | + | chr7.2:42675257-42675276 | None:intergenic | 45.0% | |
| !! | GTTGAAAGTGCTGGTGAATG+TGG | - | chr7.2:42675032-42675051 | MS.gene46067:CDS | 45.0% |
| AACCTTGCTGATCGTCCTGT+CGG | - | chr7.2:42675266-42675285 | MS.gene46067:CDS | 50.0% | |
| TCTTGTGCTGCACTTGCACA+AGG | + | chr7.2:42675098-42675117 | None:intergenic | 50.0% | |
| !! | AGTGCTGGTGAATGTGGAAG+AGG | - | chr7.2:42675038-42675057 | MS.gene46067:CDS | 50.0% |
| AACCGACAGGACGATCAGCA+AGG | + | chr7.2:42675271-42675290 | None:intergenic | 55.0% | |
| TGTCCGCACTTGTAACCGAC+AGG | + | chr7.2:42675284-42675303 | None:intergenic | 55.0% | |
| ! | CGTCCTGTCGGTTACAAGTG+CGG | - | chr7.2:42675278-42675297 | MS.gene46067:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.2 | gene | 42674966 | 42675310 | 42674966 | ID=MS.gene46067 |
| chr7.2 | mRNA | 42674966 | 42675310 | 42674966 | ID=MS.gene46067.t1;Parent=MS.gene46067 |
| chr7.2 | exon | 42674966 | 42675310 | 42674966 | ID=MS.gene46067.t1.exon1;Parent=MS.gene46067.t1 |
| chr7.2 | CDS | 42674966 | 42675310 | 42674966 | ID=cds.MS.gene46067.t1;Parent=MS.gene46067.t1 |
| Gene Sequence |
| Protein sequence |
