Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene47088.t1 | AET02751.2 | 93.5 | 108 | 7 | 0 | 37 | 144 | 1 | 108 | 3.40E-52 | 214.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene47088.t1 | G7L993 | 93.5 | 108 | 7 | 0 | 37 | 144 | 1 | 108 | 2.5e-52 | 214.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene056272 | MS.gene47088 | 0.801816 | 7.53E-49 | -1.69E-46 |
| MS.gene060634 | MS.gene47088 | -0.804946 | 1.69E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene47088.t1 | MTR_8g051620 | 93.519 | 108 | 7 | 0 | 37 | 144 | 1 | 108 | 1.63e-72 | 215 |
| MS.gene47088.t1 | MTR_8g051620 | 93.519 | 108 | 7 | 0 | 37 | 144 | 1 | 108 | 1.98e-72 | 214 |
| MS.gene47088.t1 | MTR_7g046380 | 55.488 | 164 | 72 | 1 | 15 | 178 | 162 | 324 | 4.18e-64 | 203 |
| MS.gene47088.t1 | MTR_0002s1270 | 54.878 | 164 | 73 | 1 | 15 | 178 | 154 | 316 | 2.20e-62 | 199 |
| MS.gene47088.t1 | MTR_5g077900 | 56.338 | 142 | 62 | 0 | 37 | 178 | 1 | 142 | 6.00e-58 | 182 |
| MS.gene47088.t1 | MTR_2g092960 | 33.758 | 157 | 98 | 3 | 28 | 184 | 989 | 1139 | 5.52e-24 | 99.4 |
| MS.gene47088.t1 | MTR_8g017000 | 33.824 | 136 | 84 | 2 | 32 | 167 | 462 | 591 | 1.23e-18 | 83.6 |
| MS.gene47088.t1 | MTR_2g037270 | 33.824 | 136 | 84 | 2 | 32 | 167 | 462 | 591 | 1.29e-18 | 83.6 |
| MS.gene47088.t1 | MTR_3g011560 | 34.328 | 134 | 80 | 3 | 34 | 167 | 466 | 591 | 2.05e-18 | 83.2 |
| MS.gene47088.t1 | MTR_4g066310 | 51.471 | 68 | 32 | 1 | 18 | 85 | 133 | 199 | 4.52e-18 | 79.0 |
| MS.gene47088.t1 | MTR_0462s0020 | 28.571 | 161 | 108 | 2 | 31 | 191 | 69 | 222 | 1.61e-16 | 76.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 0 sgRNAs with CRISPR-Local
Find 52 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TAAAAATTAAATTCATTTGT+AGG | + | 43113:374915-374934 | MS.gene47088:intron | 10.0% |
| !!! | ATTTAAAACTCAATTTTGTT+GGG | - | 43113:374398-374417 | None:intergenic | 15.0% |
| !!! | TATTGATTTCATATTTATTC+AGG | + | 43113:374316-374335 | MS.gene47088:intron | 15.0% |
| !! | ATTAAGAAATTTATGACTTC+CGG | - | 43113:374152-374171 | None:intergenic | 20.0% |
| !! | TATTAAAAAAAAAGGTTCGT+TGG | + | 43113:374818-374837 | MS.gene47088:intron | 20.0% |
| !!! | GATTTAAAACTCAATTTTGT+TGG | - | 43113:374399-374418 | None:intergenic | 20.0% |
| ! | AAAATGTGTTAAAGACTTGT+TGG | + | 43113:374178-374197 | MS.gene47088:CDS | 25.0% |
| ! | ACAAACAAATCAAATGTGTT+TGG | + | 43113:374363-374382 | MS.gene47088:CDS | 25.0% |
| ! | ACTATTCTATTCTACAAGTT+TGG | + | 43113:374219-374238 | MS.gene47088:CDS | 25.0% |
| ! | CTAAGGTTAAAATTAGAACA+TGG | + | 43113:374125-374144 | MS.gene47088:CDS | 25.0% |
| ! | GATCTTTGTTAAATGAACAA+AGG | - | 43113:374649-374668 | None:intergenic | 25.0% |
| ! | GCAAACGGTATTAAAAAAAA+AGG | + | 43113:374810-374829 | MS.gene47088:intron | 25.0% |
| ! | TATACTTACTCCATAAGAAA+CGG | - | 43113:374504-374523 | None:intergenic | 25.0% |
| ! | TTTCTGAACATTGTCAATAA+TGG | - | 43113:374072-374091 | None:intergenic | 25.0% |
| !! | AACAAGTCTTTAACACATTT+TGG | - | 43113:374179-374198 | None:intergenic | 25.0% |
| !!! | GTTCTATGTTTTAGTGTTAT+AGG | - | 43113:374742-374761 | None:intergenic | 25.0% |
| ACAACCAATGTATTATCCTT+TGG | - | 43113:374626-374645 | None:intergenic | 30.0% | |
| ATTAATCGTCATTTGTCCAA+AGG | + | 43113:374607-374626 | MS.gene47088:CDS | 30.0% | |
| GTTAAAATTAGAACATGGTC+CGG | + | 43113:374130-374149 | MS.gene47088:CDS | 30.0% | |
| TGAAAGAGAAGCAATATCTA+AGG | + | 43113:374108-374127 | MS.gene47088:CDS | 30.0% | |
| TGAACTCTCAATCTAAACTT+TGG | + | 43113:373941-373960 | MS.gene47088:intron | 30.0% | |
| TGTTTGTTTCAATACAGAGA+CGG | - | 43113:374350-374369 | None:intergenic | 30.0% | |
| TTGTCCAAAGGATAATACAT+TGG | + | 43113:374619-374638 | MS.gene47088:CDS | 30.0% | |
| ! | ACATCTTTTCCATTTCTGAT+TGG | + | 43113:373997-374016 | MS.gene47088:intron | 30.0% |
| ! | CATCTTTTCCATTTCTGATT+GGG | + | 43113:373998-374017 | MS.gene47088:intron | 30.0% |
| ! | TATGAACAAAGTGTGTTACT+TGG | + | 43113:374476-374495 | MS.gene47088:CDS | 30.0% |
| !!! | ATTTTTTTAACCTAGTGCTC+TGG | + | 43113:374773-374792 | MS.gene47088:intron | 30.0% |
| AGATCTTGACACAACAATGA+AGG | + | 43113:374664-374683 | MS.gene47088:CDS | 35.0% | |
| ATCTTGTCTACACATACCTT+AGG | - | 43113:374850-374869 | None:intergenic | 35.0% | |
| GAGGTTCATCAATTAGCAAA+CGG | + | 43113:374795-374814 | MS.gene47088:intron | 35.0% | |
| GTTCGTATGTAGGATTTAAC+TGG | - | 43113:374444-374463 | None:intergenic | 35.0% | |
| TAAGGTATGTGTAGACAAGA+TGG | + | 43113:374849-374868 | MS.gene47088:intron | 35.0% | |
| TGATTGGGTAGATAGCAAAA+AGG | + | 43113:374013-374032 | MS.gene47088:intron | 35.0% | |
| ! | TTTACGTGTATAGAGAGCAT+TGG | + | 43113:374582-374601 | MS.gene47088:intron | 35.0% |
| !! | AACTGGTTCTGTATTCATTG+CGG | - | 43113:374427-374446 | None:intergenic | 35.0% |
| !! | TGTGTTAAAGACTTGTTGGT+TGG | + | 43113:374182-374201 | MS.gene47088:CDS | 35.0% |
| AAGCTGATCACAAGGATGAT+TGG | + | 43113:374045-374064 | MS.gene47088:CDS | 40.0% | |
| ACGCAACTTAGTTCGTATGT+AGG | - | 43113:374454-374473 | None:intergenic | 40.0% | |
| GCTATCTACCCAATCAGAAA+TGG | - | 43113:374009-374028 | None:intergenic | 40.0% | |
| GTATGCTTTGCGAAACGATT+GGG | + | 43113:374961-374980 | MS.gene47088:CDS | 40.0% | |
| TGTATGCTTTGCGAAACGAT+TGG | + | 43113:374960-374979 | MS.gene47088:CDS | 40.0% | |
| TTACTTGGCACCGTTTCTTA+TGG | + | 43113:374491-374510 | MS.gene47088:CDS | 40.0% | |
| ! | TTTTTAACCTAGTGCTCTGG+AGG | + | 43113:374776-374795 | MS.gene47088:intron | 40.0% |
| !! | GTTAAAGACTTGTTGGTTGG+CGG | + | 43113:374185-374204 | MS.gene47088:CDS | 40.0% |
| !! | TTAAAGACTTGTTGGTTGGC+GGG | + | 43113:374186-374205 | MS.gene47088:CDS | 40.0% |
| !! | AGCTCTAGAAGCTGATCACA+AGG | + | 43113:374037-374056 | MS.gene47088:CDS | 45.0% |
| !! | TAAAGACTTGTTGGTTGGCG+GGG | + | 43113:374187-374206 | MS.gene47088:CDS | 45.0% |
| ! | GCTTGTTGGGAGCGTGAAAT+TGG | + | 43113:373871-373890 | MS.gene47088:CDS | 50.0% |
| ! | GTTGGGAGCGTGAAATTGGA+CGG | + | 43113:373875-373894 | MS.gene47088:CDS | 50.0% |
| ! | TGATGAACCTCCAGAGCACT+AGG | - | 43113:374786-374805 | None:intergenic | 50.0% |
| !! | GGTTCGTTGGCTTCGTCCTA+AGG | + | 43113:374831-374850 | MS.gene47088:intron | 55.0% |
| ! | ACTTGTTGGTTGGCGGGGCT+TGG | + | 43113:374192-374211 | MS.gene47088:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 43113 | gene | 373866 | 375015 | 373866 | ID=MS.gene47088 |
| 43113 | mRNA | 373866 | 375015 | 373866 | ID=MS.gene47088.t1;Parent=MS.gene47088 |
| 43113 | exon | 373866 | 373896 | 373866 | ID=MS.gene47088.t1.exon1;Parent=MS.gene47088.t1 |
| 43113 | CDS | 373866 | 373896 | 373866 | ID=cds.MS.gene47088.t1;Parent=MS.gene47088.t1 |
| 43113 | exon | 374024 | 374246 | 374024 | ID=MS.gene47088.t1.exon2;Parent=MS.gene47088.t1 |
| 43113 | CDS | 374024 | 374246 | 374024 | ID=cds.MS.gene47088.t1;Parent=MS.gene47088.t1 |
| 43113 | exon | 374338 | 374514 | 374338 | ID=MS.gene47088.t1.exon3;Parent=MS.gene47088.t1 |
| 43113 | CDS | 374338 | 374514 | 374338 | ID=cds.MS.gene47088.t1;Parent=MS.gene47088.t1 |
| 43113 | exon | 374595 | 374699 | 374595 | ID=MS.gene47088.t1.exon4;Parent=MS.gene47088.t1 |
| 43113 | CDS | 374595 | 374699 | 374595 | ID=cds.MS.gene47088.t1;Parent=MS.gene47088.t1 |
| 43113 | exon | 374937 | 375015 | 374937 | ID=MS.gene47088.t1.exon5;Parent=MS.gene47088.t1 |
| 43113 | CDS | 374937 | 375015 | 374937 | ID=cds.MS.gene47088.t1;Parent=MS.gene47088.t1 |
| Gene Sequence |
| Protein sequence |
