Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene47325.t1 | XP_013451561.1 | 95.5 | 179 | 8 | 0 | 1 | 179 | 142 | 320 | 2.30E-92 | 348.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene47325.t1 | P27216 | 37.3 | 166 | 102 | 2 | 1 | 166 | 151 | 314 | 3.6e-22 | 106.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene47325.t1 | A0A072U747 | 95.5 | 179 | 8 | 0 | 1 | 179 | 142 | 320 | 1.6e-92 | 348.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051744 | MS.gene47325 | 0.813594 | 2.37E-51 | -1.69E-46 |
| MS.gene053226 | MS.gene47325 | 0.808871 | 2.50E-50 | -1.69E-46 |
| MS.gene057034 | MS.gene47325 | 0.81363 | 2.32E-51 | -1.69E-46 |
| MS.gene057208 | MS.gene47325 | 0.803613 | 3.21E-49 | -1.69E-46 |
| MS.gene057915 | MS.gene47325 | 0.800164 | 1.64E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene47325.t1 | MTR_6g028030 | 95.531 | 179 | 8 | 0 | 1 | 179 | 142 | 320 | 9.03e-126 | 357 |
| MS.gene47325.t1 | MTR_6g071605 | 36.145 | 166 | 104 | 2 | 1 | 166 | 147 | 310 | 2.01e-27 | 105 |
| MS.gene47325.t1 | MTR_8g107640 | 35.542 | 166 | 105 | 2 | 1 | 166 | 147 | 310 | 2.04e-24 | 97.1 |
| MS.gene47325.t1 | MTR_6g071595 | 38.816 | 152 | 91 | 2 | 15 | 166 | 161 | 310 | 3.68e-24 | 96.7 |
| MS.gene47325.t1 | MTR_1g112520 | 34.940 | 166 | 106 | 2 | 1 | 166 | 147 | 310 | 5.03e-24 | 96.3 |
| MS.gene47325.t1 | MTR_6g071615 | 36.747 | 166 | 103 | 2 | 1 | 166 | 147 | 310 | 1.43e-23 | 95.1 |
| MS.gene47325.t1 | MTR_8g038220 | 32.544 | 169 | 111 | 2 | 1 | 169 | 147 | 312 | 6.14e-19 | 82.4 |
| MS.gene47325.t1 | MTR_8g038220 | 32.544 | 169 | 111 | 2 | 1 | 169 | 146 | 311 | 7.01e-19 | 82.4 |
| MS.gene47325.t1 | MTR_8g038180 | 31.953 | 169 | 112 | 2 | 1 | 169 | 147 | 312 | 1.34e-18 | 81.6 |
| MS.gene47325.t1 | MTR_1g033560 | 31.325 | 166 | 111 | 3 | 1 | 166 | 148 | 310 | 3.28e-18 | 80.5 |
| MS.gene47325.t1 | MTR_8g038150 | 31.361 | 169 | 113 | 2 | 1 | 169 | 151 | 316 | 5.54e-18 | 80.1 |
| MS.gene47325.t1 | MTR_3g018790 | 28.402 | 169 | 118 | 2 | 1 | 169 | 93 | 258 | 1.68e-16 | 75.1 |
| MS.gene47325.t1 | MTR_3g018790 | 28.402 | 169 | 118 | 2 | 1 | 169 | 147 | 312 | 4.11e-16 | 74.7 |
| MS.gene47325.t1 | MTR_3g018780 | 28.402 | 169 | 118 | 2 | 1 | 169 | 147 | 312 | 2.92e-15 | 72.4 |
| MS.gene47325.t1 | MTR_5g063670 | 28.313 | 166 | 116 | 2 | 1 | 166 | 148 | 310 | 1.13e-13 | 67.8 |
| MS.gene47325.t1 | MTR_6g071595 | 37.079 | 89 | 55 | 1 | 15 | 103 | 161 | 248 | 2.49e-12 | 63.9 |
| MS.gene47325.t1 | MTR_8g038210 | 27.811 | 169 | 119 | 2 | 1 | 169 | 146 | 311 | 8.39e-12 | 62.8 |
| MS.gene47325.t1 | MTR_8g038210 | 27.811 | 169 | 119 | 2 | 1 | 169 | 146 | 311 | 9.43e-12 | 62.4 |
| MS.gene47325.t1 | MTR_8g038170 | 27.059 | 170 | 112 | 4 | 1 | 166 | 148 | 309 | 1.75e-11 | 61.6 |
| MS.gene47325.t1 | MTR_8g038170 | 27.059 | 170 | 112 | 4 | 1 | 166 | 148 | 309 | 1.75e-11 | 61.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene47325.t1 | AT5G12380 | 31.928 | 166 | 110 | 2 | 1 | 166 | 147 | 309 | 7.10e-17 | 77.0 |
| MS.gene47325.t1 | AT1G68090 | 31.496 | 127 | 86 | 1 | 40 | 166 | 186 | 311 | 8.32e-15 | 71.2 |
| MS.gene47325.t1 | AT5G65020 | 29.167 | 168 | 113 | 4 | 1 | 166 | 148 | 311 | 3.39e-14 | 69.3 |
| MS.gene47325.t1 | AT5G65020 | 29.167 | 168 | 113 | 4 | 1 | 166 | 133 | 296 | 4.38e-14 | 68.9 |
| MS.gene47325.t1 | AT5G10230 | 26.506 | 166 | 119 | 2 | 1 | 166 | 148 | 310 | 8.66e-13 | 65.5 |
| MS.gene47325.t1 | AT2G38760 | 30.952 | 168 | 110 | 4 | 1 | 166 | 153 | 316 | 1.15e-12 | 65.1 |
| MS.gene47325.t1 | AT1G35720 | 27.545 | 167 | 117 | 3 | 1 | 166 | 148 | 311 | 7.80e-12 | 62.8 |
Find 34 sgRNAs with CRISPR-Local
Find 55 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTGAGTTATAAACACATTTA+TGG | 0.143306 | 6.4:-3496637 | MS.gene47325:CDS |
| TTTGATTATGCAGATTCTTA+TGG | 0.279975 | 6.4:-3496830 | None:intergenic |
| AGGGGAAATTATGGACAATT+TGG | 0.287260 | 6.4:-3496466 | MS.gene47325:CDS |
| TGAGTTATAAACACATTTAT+GGG | 0.325057 | 6.4:-3496636 | MS.gene47325:CDS |
| TCTATCAAGAGGGGAAATTA+TGG | 0.325515 | 6.4:-3496475 | MS.gene47325:CDS |
| GAAACTGGAGAGGGAAGTTT+AGG | 0.354288 | 6.4:-3496730 | MS.gene47325:CDS |
| TAATTGCTATAGATGTTAGA+AGG | 0.354460 | 6.4:+3495900 | None:intergenic |
| ACAATTTGGTAAAGCTCTAA+TGG | 0.400689 | 6.4:-3496452 | MS.gene47325:CDS |
| TAGACATGGATGAGATTAGA+AGG | 0.426400 | 6.4:-3496031 | MS.gene47325:CDS |
| GCAAGGAGGCTATATGAAAC+TGG | 0.431751 | 6.4:-3496745 | MS.gene47325:CDS |
| TAGAGACTTTCTAGTTGCCT+TGG | 0.448017 | 6.4:-3495940 | MS.gene47325:CDS |
| TGGCAAGAACATTGGTGAGC+AGG | 0.448364 | 6.4:-3496061 | MS.gene47325:CDS |
| GAAAAGTATGAGAAGGAACT+TGG | 0.506704 | 6.4:-3495999 | MS.gene47325:CDS |
| GGCAAGAACATTGGTGAGCA+GGG | 0.507512 | 6.4:-3496060 | MS.gene47325:CDS |
| CATCACACTTAGATATATGA+TGG | 0.514420 | 6.4:+3496767 | None:intergenic |
| AGGAGGTTTGGCAAGAACAT+TGG | 0.546981 | 6.4:-3496069 | MS.gene47325:CDS |
| AATTGCTATAGATGTTAGAA+GGG | 0.559492 | 6.4:+3495901 | None:intergenic |
| TATGATGGCTAATATCTGCT+TGG | 0.570576 | 6.4:+3496782 | None:intergenic |
| TTTCCAGGAAAAGTATGAGA+AGG | 0.576636 | 6.4:-3496006 | MS.gene47325:CDS |
| AACTATATGCAAGCATCAAG+AGG | 0.578258 | 6.4:-3496106 | MS.gene47325:CDS |
| TCAAGCATATTATTATGCAA+AGG | 0.609590 | 6.4:-3496407 | MS.gene47325:intron |
| ATGTGAAAGCATTCCATGTG+GGG | 0.625778 | 6.4:-3495967 | MS.gene47325:CDS |
| TATGTGAAAGCATTCCATGT+GGG | 0.626705 | 6.4:-3495968 | MS.gene47325:CDS |
| ACTATATGCAAGCATCAAGA+GGG | 0.628173 | 6.4:-3496105 | MS.gene47325:CDS |
| AGGCTATATGAAACTGGAGA+GGG | 0.640552 | 6.4:-3496739 | MS.gene47325:CDS |
| ATTTGGTAAAGCTCTAATGG+TGG | 0.647291 | 6.4:-3496449 | MS.gene47325:CDS |
| GAGGCTATATGAAACTGGAG+AGG | 0.649820 | 6.4:-3496740 | MS.gene47325:CDS |
| ATATATCTAAGTGTGATGCA+AGG | 0.685196 | 6.4:-3496762 | MS.gene47325:CDS |
| GAGCAGGGCAGATATAGACA+TGG | 0.686297 | 6.4:-3496045 | MS.gene47325:CDS |
| AAGTCTCTATAATCCCCACA+TGG | 0.697353 | 6.4:+3495954 | None:intergenic |
| ATTGCTATAGATGTTAGAAG+GGG | 0.699113 | 6.4:+3495902 | None:intergenic |
| TATCTAAGTGTGATGCAAGG+AGG | 0.713979 | 6.4:-3496759 | MS.gene47325:CDS |
| TTATGGGCATGACTACACAA+AGG | 0.754823 | 6.4:-3496620 | MS.gene47325:intron |
| ATATGTGAAAGCATTCCATG+TGG | 0.785559 | 6.4:-3495969 | MS.gene47325:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TAATGAAAATAGATTAAATG+AGG | + | chr6.4:3496167-3496186 | None:intergenic | 15.0% |
| !!! | AAAAGTAAAAACACAATTTT+TGG | + | chr6.4:3496420-3496439 | None:intergenic | 15.0% |
| !!! | TTAACAATTTGAGTTAATAA+TGG | - | chr6.4:3496583-3496602 | MS.gene47325:intron | 15.0% |
| !! | AAGAAACAAAGATTAGAAAA+AGG | + | chr6.4:3496339-3496358 | None:intergenic | 20.0% |
| !! | AGAAACAAAGATTAGAAAAA+GGG | + | chr6.4:3496338-3496357 | None:intergenic | 20.0% |
| !! | CAAATATTGCTAACTTTAAT+CGG | - | chr6.4:3496520-3496539 | MS.gene47325:intron | 20.0% |
| !! | TACAATTCTAATATTCGATT+AGG | + | chr6.4:3496563-3496582 | None:intergenic | 20.0% |
| !! | TGAGTTATAAACACATTTAT+GGG | - | chr6.4:3496080-3496099 | MS.gene47325:CDS | 20.0% |
| !! | TTGAGTTATAAACACATTTA+TGG | - | chr6.4:3496079-3496098 | MS.gene47325:CDS | 20.0% |
| !!! | AAAAATGTTAGTTTCAATTG+TGG | + | chr6.4:3496061-3496080 | None:intergenic | 20.0% |
| !!! | AAGTTAGCAATATTTGATTT+CGG | + | chr6.4:3496516-3496535 | None:intergenic | 20.0% |
| !!! | TCTTCTACAAATTCTAATTT+TGG | - | chr6.4:3496355-3496374 | MS.gene47325:intron | 20.0% |
| ! | AATTGCTATAGATGTTAGAA+GGG | + | chr6.4:3496818-3496837 | None:intergenic | 25.0% |
| ! | TAATTGCTATAGATGTTAGA+AGG | + | chr6.4:3496819-3496838 | None:intergenic | 25.0% |
| ! | TCAAGCATATTATTATGCAA+AGG | - | chr6.4:3496309-3496328 | MS.gene47325:intron | 25.0% |
| !!! | TTTGTTTTAGTCTATCAAGA+GGG | - | chr6.4:3496231-3496250 | MS.gene47325:intron | 25.0% |
| !!! | TTTTGTTTTAGTCTATCAAG+AGG | - | chr6.4:3496230-3496249 | MS.gene47325:intron | 25.0% |
| ATATATCTAAGTGTGATGCA+AGG | - | chr6.4:3495954-3495973 | MS.gene47325:CDS | 30.0% | |
| ATGAGTGACTAAAAACACAT+TGG | - | chr6.4:3496457-3496476 | MS.gene47325:CDS | 30.0% | |
| ATTGCTATAGATGTTAGAAG+GGG | + | chr6.4:3496817-3496836 | None:intergenic | 30.0% | |
| CATCACACTTAGATATATGA+TGG | + | chr6.4:3495952-3495971 | None:intergenic | 30.0% | |
| TAAATGAGGCAAGAAAAAAG+AGG | + | chr6.4:3496153-3496172 | None:intergenic | 30.0% | |
| ! | ACAATTTGGTAAAGCTCTAA+TGG | - | chr6.4:3496264-3496283 | MS.gene47325:intron | 30.0% |
| ! | CAATTTGAGTTAATAATGGC+AGG | - | chr6.4:3496587-3496606 | MS.gene47325:intron | 30.0% |
| !!! | TTGTTTTAGTCTATCAAGAG+GGG | - | chr6.4:3496232-3496251 | MS.gene47325:intron | 30.0% |
| AAAAAGAGACATAGGAGGTT+TGG | - | chr6.4:3496635-3496654 | MS.gene47325:CDS | 35.0% | |
| AACTATATGCAAGCATCAAG+AGG | - | chr6.4:3496610-3496629 | MS.gene47325:intron | 35.0% | |
| AAGAGGGAAAAAAGAGACAT+AGG | - | chr6.4:3496627-3496646 | MS.gene47325:CDS | 35.0% | |
| ACTATATGCAAGCATCAAGA+GGG | - | chr6.4:3496611-3496630 | MS.gene47325:intron | 35.0% | |
| AGGGGAAATTATGGACAATT+TGG | - | chr6.4:3496250-3496269 | MS.gene47325:intron | 35.0% | |
| TAGACATGGATGAGATTAGA+AGG | - | chr6.4:3496685-3496704 | MS.gene47325:CDS | 35.0% | |
| TATGATGGCTAATATCTGCT+TGG | + | chr6.4:3495937-3495956 | None:intergenic | 35.0% | |
| TCTATCAAGAGGGGAAATTA+TGG | - | chr6.4:3496241-3496260 | MS.gene47325:intron | 35.0% | |
| ! | ATTTGGTAAAGCTCTAATGG+TGG | - | chr6.4:3496267-3496286 | MS.gene47325:intron | 35.0% |
| ! | GAAAAGTATGAGAAGGAACT+TGG | - | chr6.4:3496717-3496736 | MS.gene47325:CDS | 35.0% |
| ! | TGAGATTAGAAGGATTTTCC+AGG | - | chr6.4:3496695-3496714 | MS.gene47325:CDS | 35.0% |
| ! | TTTCCAGGAAAAGTATGAGA+AGG | - | chr6.4:3496710-3496729 | MS.gene47325:CDS | 35.0% |
| !! | ATATGTGAAAGCATTCCATG+TGG | - | chr6.4:3496747-3496766 | MS.gene47325:CDS | 35.0% |
| !! | TATGTGAAAGCATTCCATGT+GGG | - | chr6.4:3496748-3496767 | MS.gene47325:CDS | 35.0% |
| AAGTCTCTATAATCCCCACA+TGG | + | chr6.4:3496765-3496784 | None:intergenic | 40.0% | |
| AGGCTATATGAAACTGGAGA+GGG | - | chr6.4:3495977-3495996 | MS.gene47325:CDS | 40.0% | |
| AGGGAAAAAAGAGACATAGG+AGG | - | chr6.4:3496630-3496649 | MS.gene47325:CDS | 40.0% | |
| TATCTAAGTGTGATGCAAGG+AGG | - | chr6.4:3495957-3495976 | MS.gene47325:CDS | 40.0% | |
| TTATGGGCATGACTACACAA+AGG | - | chr6.4:3496096-3496115 | MS.gene47325:CDS | 40.0% | |
| ! | GGTACAAGATTTTGTAGCCA+AGG | + | chr6.4:3496796-3496815 | None:intergenic | 40.0% |
| ! | GTTCCTTCTCATACTTTTCC+TGG | + | chr6.4:3496716-3496735 | None:intergenic | 40.0% |
| !! | ATGTGAAAGCATTCCATGTG+GGG | - | chr6.4:3496749-3496768 | MS.gene47325:CDS | 40.0% |
| !! | TAGAGACTTTCTAGTTGCCT+TGG | - | chr6.4:3496776-3496795 | MS.gene47325:CDS | 40.0% |
| AGGAGGTTTGGCAAGAACAT+TGG | - | chr6.4:3496647-3496666 | MS.gene47325:CDS | 45.0% | |
| GAAACTGGAGAGGGAAGTTT+AGG | - | chr6.4:3495986-3496005 | MS.gene47325:CDS | 45.0% | |
| GAGGCTATATGAAACTGGAG+AGG | - | chr6.4:3495976-3495995 | MS.gene47325:CDS | 45.0% | |
| GCAAGGAGGCTATATGAAAC+TGG | - | chr6.4:3495971-3495990 | MS.gene47325:CDS | 45.0% | |
| GAGCAGGGCAGATATAGACA+TGG | - | chr6.4:3496671-3496690 | MS.gene47325:CDS | 50.0% | |
| GGCAAGAACATTGGTGAGCA+GGG | - | chr6.4:3496656-3496675 | MS.gene47325:CDS | 50.0% | |
| TGGCAAGAACATTGGTGAGC+AGG | - | chr6.4:3496655-3496674 | MS.gene47325:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.4 | gene | 3495899 | 3496839 | 3495899 | ID=MS.gene47325 |
| chr6.4 | mRNA | 3495899 | 3496839 | 3495899 | ID=MS.gene47325.t1;Parent=MS.gene47325 |
| chr6.4 | exon | 3496621 | 3496839 | 3496621 | ID=MS.gene47325.t1.exon1;Parent=MS.gene47325.t1 |
| chr6.4 | CDS | 3496621 | 3496839 | 3496621 | ID=cds.MS.gene47325.t1;Parent=MS.gene47325.t1 |
| chr6.4 | exon | 3496408 | 3496497 | 3496408 | ID=MS.gene47325.t1.exon2;Parent=MS.gene47325.t1 |
| chr6.4 | CDS | 3496408 | 3496497 | 3496408 | ID=cds.MS.gene47325.t1;Parent=MS.gene47325.t1 |
| chr6.4 | exon | 3495899 | 3496129 | 3495899 | ID=MS.gene47325.t1.exon3;Parent=MS.gene47325.t1 |
| chr6.4 | CDS | 3495899 | 3496129 | 3495899 | ID=cds.MS.gene47325.t1;Parent=MS.gene47325.t1 |
| Gene Sequence |
| Protein sequence |
