Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene47521.t1 | XP_024637096.1 | 98.9 | 188 | 2 | 0 | 20 | 207 | 29 | 216 | 5.00E-75 | 290.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene47521.t1 | G7JNM4 | 98.9 | 188 | 2 | 0 | 20 | 207 | 53 | 240 | 3.6e-75 | 290.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049418 | MS.gene47521 | 0.829829 | 4.14E-55 | -1.69E-46 |
| MS.gene049989 | MS.gene47521 | 0.814443 | 1.54E-51 | -1.69E-46 |
| MS.gene051067 | MS.gene47521 | 0.804437 | 2.16E-49 | -1.69E-46 |
| MS.gene058197 | MS.gene47521 | 0.81349 | 2.49E-51 | -1.69E-46 |
| MS.gene058198 | MS.gene47521 | 0.8103 | 1.23E-50 | -1.69E-46 |
| MS.gene059300 | MS.gene47521 | 0.825056 | 5.77E-54 | -1.69E-46 |
| MS.gene06057 | MS.gene47521 | 0.808947 | 2.41E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene47521.t1 | MTR_4g087380 | 98.936 | 188 | 2 | 0 | 20 | 207 | 53 | 240 | 3.36e-137 | 384 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene47521.t1 | AT3G59680 | 53.299 | 197 | 78 | 8 | 15 | 203 | 5 | 195 | 2.88e-58 | 182 |
| MS.gene47521.t1 | AT3G59680 | 54.688 | 192 | 73 | 8 | 20 | 203 | 38 | 223 | 6.03e-58 | 182 |
Find 36 sgRNAs with CRISPR-Local
Find 45 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CATCTAATAATTCAACTTTA+TGG | 0.128033 | 4.3:-25805377 | MS.gene47521:CDS |
| GTGGAAATATTTGTTGATTT+TGG | 0.165410 | 4.3:+25805399 | None:intergenic |
| ATTTCTCAAAATCTCATTAT+AGG | 0.182353 | 4.3:+25805224 | None:intergenic |
| GTAGGAACTTGAGGGGATTT+TGG | 0.233877 | 4.3:+25805345 | None:intergenic |
| GAAATCAAATGGAAGTTATT+AGG | 0.286022 | 4.3:-25805002 | MS.gene47521:CDS |
| GGATAACTATTACTTCTTCT+AGG | 0.341829 | 4.3:+25805192 | None:intergenic |
| ATGGTTCTTGCAGATGATAT+TGG | 0.388098 | 4.3:-25804928 | MS.gene47521:CDS |
| CAATGGATGTAACTAGCTTA+TGG | 0.393769 | 4.3:-25805431 | MS.gene47521:CDS |
| ATCTCATTATAGGAATAAAT+AGG | 0.401100 | 4.3:+25805234 | None:intergenic |
| TTCAATTTATTTCAAGATGC+AGG | 0.417350 | 4.3:-25805473 | MS.gene47521:CDS |
| AAGATTTGTTATTGAGGAAG+AGG | 0.427819 | 4.3:-25805046 | MS.gene47521:CDS |
| GCCTAGTCCTCTTAGTAGAA+TGG | 0.460019 | 4.3:-25804947 | MS.gene47521:CDS |
| AATGAAAAGATTTGTTATTG+AGG | 0.466620 | 4.3:-25805052 | MS.gene47521:CDS |
| AAATGGAAGTTATTAGGAAA+AGG | 0.469401 | 4.3:-25804996 | MS.gene47521:CDS |
| AAGAAAATGTACATCTCTAA+AGG | 0.469811 | 4.3:-25805292 | MS.gene47521:CDS |
| AACTTTGTGTAGGAACTTGA+GGG | 0.480059 | 4.3:+25805337 | None:intergenic |
| TCCATTGCTCTTTGATACCA+TGG | 0.496795 | 4.3:+25805447 | None:intergenic |
| AATTAGAAGAAGAAATCAAA+TGG | 0.496957 | 4.3:-25805013 | MS.gene47521:CDS |
| GAACTTTGTGTAGGAACTTG+AGG | 0.507052 | 4.3:+25805336 | None:intergenic |
| GCAAGAACCATTCTACTAAG+AGG | 0.510476 | 4.3:+25804940 | None:intergenic |
| TAAAGTTGAATTATTAGATG+TGG | 0.519784 | 4.3:+25805380 | None:intergenic |
| TCATGTGATGTTGTTTGCAA+TGG | 0.526646 | 4.3:+25805156 | None:intergenic |
| TAATAATTCAACTTTATGGA+AGG | 0.536189 | 4.3:-25805373 | MS.gene47521:CDS |
| TTTCAAGATGCAGGAAGCCA+TGG | 0.559134 | 4.3:-25805464 | MS.gene47521:CDS |
| TTGTTATTAGAACTTTGTGT+AGG | 0.560547 | 4.3:+25805327 | None:intergenic |
| GCCATGGTATCAAAGAGCAA+TGG | 0.567750 | 4.3:-25805448 | MS.gene47521:CDS |
| AAATTAGAACTCATAGTGCT+AGG | 0.568662 | 4.3:-25805134 | MS.gene47521:CDS |
| AGTGCTAGGCTTAGCACAGA+GGG | 0.581749 | 4.3:-25805120 | MS.gene47521:CDS |
| TGCATTAGTACTTCTTGAGA+TGG | 0.609173 | 4.3:+25805504 | None:intergenic |
| AATTGGTTCTAAAATGAATG+TGG | 0.611189 | 4.3:-25805532 | None:intergenic |
| ACTTTGTGTAGGAACTTGAG+GGG | 0.629081 | 4.3:+25805338 | None:intergenic |
| TAACTATTACTTCTTCTAGG+TGG | 0.635383 | 4.3:+25805195 | None:intergenic |
| ACCATTCTACTAAGAGGACT+AGG | 0.640553 | 4.3:+25804946 | None:intergenic |
| TTAGGAAAAGGAGTGTGATG+AGG | 0.650109 | 4.3:-25804984 | MS.gene47521:CDS |
| TTAGCACAGAGGGATCAAGA+AGG | 0.686930 | 4.3:-25805110 | MS.gene47521:CDS |
| TAGTGCTAGGCTTAGCACAG+AGG | 0.719730 | 4.3:-25805121 | MS.gene47521:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATTAGAAGAAGAAATCAAA+TGG | - | chr4.3:25805424-25805443 | MS.gene47521:CDS | 20.0% |
| !! | ATCTCATTATAGGAATAAAT+AGG | + | chr4.3:25805206-25805225 | None:intergenic | 20.0% |
| !! | ATTTCTCAAAATCTCATTAT+AGG | + | chr4.3:25805216-25805235 | None:intergenic | 20.0% |
| !! | CATCTAATAATTCAACTTTA+TGG | - | chr4.3:25805060-25805079 | MS.gene47521:CDS | 20.0% |
| !! | TAAAGTTGAATTATTAGATG+TGG | + | chr4.3:25805060-25805079 | None:intergenic | 20.0% |
| !! | TAATAATTCAACTTTATGGA+AGG | - | chr4.3:25805064-25805083 | MS.gene47521:CDS | 20.0% |
| !!! | AATGAAAAGATTTGTTATTG+AGG | - | chr4.3:25805385-25805404 | MS.gene47521:CDS | 20.0% |
| ! | AAATGGAAGTTATTAGGAAA+AGG | - | chr4.3:25805441-25805460 | MS.gene47521:CDS | 25.0% |
| ! | AAGAAAATGTACATCTCTAA+AGG | - | chr4.3:25805145-25805164 | MS.gene47521:CDS | 25.0% |
| ! | GAAATCAAATGGAAGTTATT+AGG | - | chr4.3:25805435-25805454 | MS.gene47521:CDS | 25.0% |
| ! | TTCAATTTATTTCAAGATGC+AGG | - | chr4.3:25804964-25804983 | MS.gene47521:CDS | 25.0% |
| !! | TTGTTATTAGAACTTTGTGT+AGG | + | chr4.3:25805113-25805132 | None:intergenic | 25.0% |
| !!! | GTGGAAATATTTGTTGATTT+TGG | + | chr4.3:25805041-25805060 | None:intergenic | 25.0% |
| !!! | TAATGAGATTTTGAGAAATG+AGG | - | chr4.3:25805217-25805236 | MS.gene47521:CDS | 25.0% |
| AAATTAGAACTCATAGTGCT+AGG | - | chr4.3:25805303-25805322 | MS.gene47521:CDS | 30.0% | |
| GGATAACTATTACTTCTTCT+AGG | + | chr4.3:25805248-25805267 | None:intergenic | 30.0% | |
| TAACTATTACTTCTTCTAGG+TGG | + | chr4.3:25805245-25805264 | None:intergenic | 30.0% | |
| ! | AGATGTACATTTTCTTAGCT+TGG | + | chr4.3:25805141-25805160 | None:intergenic | 30.0% |
| !! | AAGATTTGTTATTGAGGAAG+AGG | - | chr4.3:25805391-25805410 | MS.gene47521:CDS | 30.0% |
| AACTTTGTGTAGGAACTTGA+GGG | + | chr4.3:25805103-25805122 | None:intergenic | 35.0% | |
| ATGGTTCTTGCAGATGATAT+TGG | - | chr4.3:25805509-25805528 | MS.gene47521:CDS | 35.0% | |
| TGATGAGGAAAAAGAGACTT+GGG | - | chr4.3:25805468-25805487 | MS.gene47521:CDS | 35.0% | |
| TGCATTAGTACTTCTTGAGA+TGG | + | chr4.3:25804936-25804955 | None:intergenic | 35.0% | |
| ! | AGGGATCAAGAAGGATTTTT+AGG | - | chr4.3:25805336-25805355 | MS.gene47521:CDS | 35.0% |
| ! | AGGTAGCAACATCTTTTACA+AGG | - | chr4.3:25805165-25805184 | MS.gene47521:CDS | 35.0% |
| ! | CAATGGATGTAACTAGCTTA+TGG | - | chr4.3:25805006-25805025 | MS.gene47521:CDS | 35.0% |
| ! | GGGATCAAGAAGGATTTTTA+GGG | - | chr4.3:25805337-25805356 | MS.gene47521:CDS | 35.0% |
| ! | GGTAGCAACATCTTTTACAA+GGG | - | chr4.3:25805166-25805185 | MS.gene47521:CDS | 35.0% |
| ! | TCATGTGATGTTGTTTGCAA+TGG | + | chr4.3:25805284-25805303 | None:intergenic | 35.0% |
| !! | GGATCAAGAAGGATTTTTAG+GGG | - | chr4.3:25805338-25805357 | MS.gene47521:CDS | 35.0% |
| !!! | TGCAATGGTTTTGATGATCT+AGG | + | chr4.3:25805269-25805288 | None:intergenic | 35.0% |
| ACCATTCTACTAAGAGGACT+AGG | + | chr4.3:25805494-25805513 | None:intergenic | 40.0% | |
| ACTTTGTGTAGGAACTTGAG+GGG | + | chr4.3:25805102-25805121 | None:intergenic | 40.0% | |
| GAACTTTGTGTAGGAACTTG+AGG | + | chr4.3:25805104-25805123 | None:intergenic | 40.0% | |
| GCAAGAACCATTCTACTAAG+AGG | + | chr4.3:25805500-25805519 | None:intergenic | 40.0% | |
| GTGATGAGGAAAAAGAGACT+TGG | - | chr4.3:25805467-25805486 | MS.gene47521:CDS | 40.0% | |
| TCCATTGCTCTTTGATACCA+TGG | + | chr4.3:25804993-25805012 | None:intergenic | 40.0% | |
| ! | TTAGGAAAAGGAGTGTGATG+AGG | - | chr4.3:25805453-25805472 | MS.gene47521:CDS | 40.0% |
| GCCATGGTATCAAAGAGCAA+TGG | - | chr4.3:25804989-25805008 | MS.gene47521:CDS | 45.0% | |
| GCCTAGTCCTCTTAGTAGAA+TGG | - | chr4.3:25805490-25805509 | MS.gene47521:CDS | 45.0% | |
| TTAGCACAGAGGGATCAAGA+AGG | - | chr4.3:25805327-25805346 | MS.gene47521:CDS | 45.0% | |
| TTTCAAGATGCAGGAAGCCA+TGG | - | chr4.3:25804973-25804992 | MS.gene47521:CDS | 45.0% | |
| ! | GTAGGAACTTGAGGGGATTT+TGG | + | chr4.3:25805095-25805114 | None:intergenic | 45.0% |
| AGTGCTAGGCTTAGCACAGA+GGG | - | chr4.3:25805317-25805336 | MS.gene47521:CDS | 50.0% | |
| TAGTGCTAGGCTTAGCACAG+AGG | - | chr4.3:25805316-25805335 | MS.gene47521:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.3 | gene | 25804918 | 25805541 | 25804918 | ID=MS.gene47521 |
| chr4.3 | mRNA | 25804918 | 25805541 | 25804918 | ID=MS.gene47521.t1;Parent=MS.gene47521 |
| chr4.3 | exon | 25804918 | 25805541 | 25804918 | ID=MS.gene47521.t1.exon1;Parent=MS.gene47521.t1 |
| chr4.3 | CDS | 25804918 | 25805541 | 25804918 | ID=cds.MS.gene47521.t1;Parent=MS.gene47521.t1 |
| Gene Sequence |
| Protein sequence |
