AlfalfaGEDB | MS.gene47666

M. sativa cultivar XinJiangDaYe / MS.gene47666

KEGG: -
GO: -
PFAM: PF05641.15

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene47666.t1	XP_003617216.1	96.6	146	5	0	1	146	1	146	1.50E-78	302

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene47666.t1	Q500V5	31.6	133	86	2	10	142	221	348	2.8e-12	73.2

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene47666.t1	G7KCV4	96.6	146	5	0	1	146	1	146	1.1e-78	302.0

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene052447	MS.gene47666	0.813564	2.40E-51	-1.69E-46
MS.gene058294	MS.gene47666	0.800203	1.61E-48	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene47666.t1	MTR_5g089140	96.575	146	5	0	1	146	1	146	5.59e-103	291
MS.gene47666.t1	MTR_5g089250	94.521	146	8	0	1	146	1	146	5.75e-100	283
MS.gene47666.t1	MTR_5g089220	92.466	146	11	0	1	146	1	146	9.66e-98	278
MS.gene47666.t1	MTR_4g079980	86.260	131	18	0	1	131	1	131	2.57e-79	231
MS.gene47666.t1	MTR_3g065560	63.014	146	54	0	1	146	1	146	1.47e-62	189
MS.gene47666.t1	MTR_4g079830	60.544	147	57	1	1	146	1	147	8.43e-61	184
MS.gene47666.t1	MTR_1g019680	58.519	135	40	1	1	135	1	119	1.17e-49	155
MS.gene47666.t1	MTR_4g127390	58.741	143	59	0	1	143	1	143	1.56e-49	155
MS.gene47666.t1	MTR_2g011700	67.826	115	23	1	1	115	1	101	1.34e-48	153
MS.gene47666.t1	MTR_1g084210	35.036	137	84	3	10	146	56	187	1.23e-20	83.6
MS.gene47666.t1	MTR_2g033390	32.090	134	90	1	13	146	258	390	2.15e-19	83.6
MS.gene47666.t1	MTR_2g033390	34.028	144	89	4	4	146	61	199	2.91e-18	80.5

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene47666.t1	AT1G06340	45.113	133	65	4	13	143	6	132	8.38e-31	108
MS.gene47666.t1	AT1G09320	35.821	134	82	2	10	143	380	509	2.50e-18	80.9
MS.gene47666.t1	AT1G09320	32.331	133	85	2	10	142	221	348	3.08e-15	72.0
MS.gene47666.t1	AT1G09320	31.915	141	83	5	11	142	40	176	4.68e-12	62.8
MS.gene47666.t1	AT3G06520	34.667	150	86	4	2	142	317	463	7.20e-15	70.9
MS.gene47666.t1	AT3G06520	29.787	141	95	3	6	144	165	303	2.77e-12	63.2
MS.gene47666.t1	AT3G06520	34.667	150	86	4	2	142	317	463	7.20e-15	70.9
MS.gene47666.t1	AT3G06520	29.787	141	95	3	6	144	165	303	2.77e-12	63.2
MS.gene47666.t1	AT1G26540	35.556	135	74	6	16	143	14	142	3.82e-13	65.9
MS.gene47666.t1	AT2G47230	34.328	134	78	6	13	142	8	135	5.27e-12	62.8
MS.gene47666.t1	AT2G47230	34.328	134	78	6	13	142	8	135	5.98e-12	62.4
MS.gene47666.t1	AT5G58610	33.333	132	67	3	2	113	18	148	6.54e-11	59.7
MS.gene47666.t1	AT5G58610	33.333	132	67	3	2	113	18	148	6.54e-11	59.7
MS.gene47666.t1	AT5G58610	33.333	132	67	3	2	113	18	148	7.02e-11	59.3
MS.gene47666.t1	AT5G58610	33.333	132	67	3	2	113	18	148	7.04e-11	59.3

Find 36 sgRNAs with CRISPR-Local

Find 34 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ACTACCCTTGTGATCAGATT+AGG	0.306305	5.2:-75717523	MS.gene47666:CDS
CTACCCTTGTGATCAGATTA+GGG	0.322716	5.2:-75717522	MS.gene47666:CDS
TGATGAGTCAGAACTTCTTA+TGG	0.340417	5.2:-75717738	MS.gene47666:CDS
AGGCAATGGACGAAGATCTT+TGG	0.373055	5.2:+75717703	None:intergenic
AAGAATTGGTTTGGGGAGAC+TGG	0.383581	5.2:-75717493	MS.gene47666:CDS
GGGTTCATCAAGAATTGGTT+TGG	0.383607	5.2:-75717502	MS.gene47666:CDS
GGTTCATCAAGAATTGGTTT+GGG	0.383908	5.2:-75717501	MS.gene47666:CDS
GATTAGGGTTCATCAAGAAT+TGG	0.399407	5.2:-75717507	MS.gene47666:CDS
AAGAGAGTTGATTACAAGGT+AGG	0.420875	5.2:-75717881	MS.gene47666:CDS
ATTGTTTCATGTCTTGATAG+TGG	0.420973	5.2:-75717797	MS.gene47666:CDS
ATAACGATGGTTGGTGGGTA+GGG	0.425281	5.2:-75717625	MS.gene47666:CDS
AAATCGCTAGCGAGAAGATC+TGG	0.430422	5.2:-75717601	MS.gene47666:CDS
AAGGGTAGTAAATAGTTTGA+TGG	0.461531	5.2:+75717537	None:intergenic
GAAGTATGCAGCAAAGAAGA+AGG	0.467318	5.2:-75717848	MS.gene47666:CDS
GATAACGATGGTTGGTGGGT+AGG	0.471553	5.2:-75717626	MS.gene47666:CDS
AGCAAAGAAGAAGGGTTTGT+AGG	0.471858	5.2:-75717839	MS.gene47666:CDS
TGATTGAGTTCAAACTTTGA+AGG	0.492804	5.2:+75717665	None:intergenic
CTTTAAGAAGATTCTTGTAA+CGG	0.514418	5.2:+75717762	None:intergenic
TCTCTTCACCGGTGGACGCA+TGG	0.520892	5.2:+75717898	None:intergenic
TTGTGCAACCATGCGTCCAC+CGG	0.536842	5.2:-75717906	None:intergenic
TTGGGGAGACTGGATCTTCG+AGG	0.539539	5.2:-75717483	MS.gene47666:CDS
TTGTAATCAACTCTCTTCAC+CGG	0.572999	5.2:+75717887	None:intergenic
TTACGAACACATGGAGGCAA+TGG	0.580919	5.2:+75717689	None:intergenic
TGTTTGATAACGATGGTTGG+TGG	0.594111	5.2:-75717631	MS.gene47666:CDS
AAGTATGCAGCAAAGAAGAA+GGG	0.604561	5.2:-75717847	MS.gene47666:CDS
CGCTAGCGAGAAGATCTGGA+TGG	0.613449	5.2:-75717597	MS.gene47666:CDS
TAATCAACTCTCTTCACCGG+TGG	0.621238	5.2:+75717890	None:intergenic
ACGTGTTTGATAACGATGGT+TGG	0.621604	5.2:-75717634	MS.gene47666:CDS
TTTGAAGGATTACGAACACA+TGG	0.623827	5.2:+75717680	None:intergenic
TGAACCCTAATCTGATCACA+AGG	0.626143	5.2:+75717518	None:intergenic
GTTCATCAAGAATTGGTTTG+GGG	0.626950	5.2:-75717500	MS.gene47666:CDS
GTTTGATAACGATGGTTGGT+GGG	0.640800	5.2:-75717630	MS.gene47666:CDS
GGTGAAGAGAGTTGATTACA+AGG	0.672184	5.2:-75717885	MS.gene47666:CDS
GAAGGATTACGAACACATGG+AGG	0.708273	5.2:+75717683	None:intergenic
GTTGACGTGTTTGATAACGA+TGG	0.717711	5.2:-75717638	MS.gene47666:CDS
GAACCCTAATCTGATCACAA+GGG	0.736467	5.2:+75717519	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	CTTTAAGAAGATTCTTGTAA+CGG	+	chr5.2:75717615-75717634	None:intergenic	25.0%
	AAGGGTAGTAAATAGTTTGA+TGG	+	chr5.2:75717840-75717859	None:intergenic	30.0%
	ATTGTTTCATGTCTTGATAG+TGG	-	chr5.2:75717577-75717596	MS.gene47666:CDS	30.0%
	TGATTGAGTTCAAACTTTGA+AGG	+	chr5.2:75717712-75717731	None:intergenic	30.0%
	AAGTATGCAGCAAAGAAGAA+GGG	-	chr5.2:75717527-75717546	MS.gene47666:CDS	35.0%
	GATTAGGGTTCATCAAGAAT+TGG	-	chr5.2:75717867-75717886	MS.gene47666:CDS	35.0%
	TGATGAGTCAGAACTTCTTA+TGG	-	chr5.2:75717636-75717655	MS.gene47666:CDS	35.0%
	TTGTAATCAACTCTCTTCAC+CGG	+	chr5.2:75717490-75717509	None:intergenic	35.0%
	TTTGAAGGATTACGAACACA+TGG	+	chr5.2:75717697-75717716	None:intergenic	35.0%
!	AAGAGAGTTGATTACAAGGT+AGG	-	chr5.2:75717493-75717512	MS.gene47666:CDS	35.0%
!	GGTTCATCAAGAATTGGTTT+GGG	-	chr5.2:75717873-75717892	MS.gene47666:CDS	35.0%
!!	GTTCATCAAGAATTGGTTTG+GGG	-	chr5.2:75717874-75717893	MS.gene47666:CDS	35.0%
	ACTACCCTTGTGATCAGATT+AGG	-	chr5.2:75717851-75717870	MS.gene47666:CDS	40.0%
	AGCAAAGAAGAAGGGTTTGT+AGG	-	chr5.2:75717535-75717554	MS.gene47666:CDS	40.0%
	CTACCCTTGTGATCAGATTA+GGG	-	chr5.2:75717852-75717871	MS.gene47666:CDS	40.0%
	GAACCCTAATCTGATCACAA+GGG	+	chr5.2:75717858-75717877	None:intergenic	40.0%
	GAAGTATGCAGCAAAGAAGA+AGG	-	chr5.2:75717526-75717545	MS.gene47666:CDS	40.0%
	GGGTTCATCAAGAATTGGTT+TGG	-	chr5.2:75717872-75717891	MS.gene47666:CDS	40.0%
	TGAACCCTAATCTGATCACA+AGG	+	chr5.2:75717859-75717878	None:intergenic	40.0%
!	GGTGAAGAGAGTTGATTACA+AGG	-	chr5.2:75717489-75717508	MS.gene47666:CDS	40.0%
!!	ACGTGTTTGATAACGATGGT+TGG	-	chr5.2:75717740-75717759	MS.gene47666:CDS	40.0%
!!	GTTGACGTGTTTGATAACGA+TGG	-	chr5.2:75717736-75717755	MS.gene47666:CDS	40.0%
!!	GTTTGATAACGATGGTTGGT+GGG	-	chr5.2:75717744-75717763	MS.gene47666:CDS	40.0%
!!	TGTTTGATAACGATGGTTGG+TGG	-	chr5.2:75717743-75717762	MS.gene47666:CDS	40.0%
	AAATCGCTAGCGAGAAGATC+TGG	-	chr5.2:75717773-75717792	MS.gene47666:CDS	45.0%
	AGGCAATGGACGAAGATCTT+TGG	+	chr5.2:75717674-75717693	None:intergenic	45.0%
	GAAGGATTACGAACACATGG+AGG	+	chr5.2:75717694-75717713	None:intergenic	45.0%
	TAATCAACTCTCTTCACCGG+TGG	+	chr5.2:75717487-75717506	None:intergenic	45.0%
	TTACGAACACATGGAGGCAA+TGG	+	chr5.2:75717688-75717707	None:intergenic	45.0%
!!	AAGAATTGGTTTGGGGAGAC+TGG	-	chr5.2:75717881-75717900	MS.gene47666:CDS	45.0%
!!	ATAACGATGGTTGGTGGGTA+GGG	-	chr5.2:75717749-75717768	MS.gene47666:CDS	45.0%
!!	GATAACGATGGTTGGTGGGT+AGG	-	chr5.2:75717748-75717767	MS.gene47666:CDS	50.0%
	CGCTAGCGAGAAGATCTGGA+TGG	-	chr5.2:75717777-75717796	MS.gene47666:CDS	55.0%
	TTGGGGAGACTGGATCTTCG+AGG	-	chr5.2:75717891-75717910	MS.gene47666:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.2	gene	75717478	75717918	75717478	ID=MS.gene47666
chr5.2	mRNA	75717478	75717918	75717478	ID=MS.gene47666.t1;Parent=MS.gene47666
chr5.2	exon	75717478	75717918	75717478	ID=MS.gene47666.t1.exon1;Parent=MS.gene47666.t1
chr5.2	CDS	75717478	75717918	75717478	ID=cds.MS.gene47666.t1;Parent=MS.gene47666.t1

Gene Sequence

>MS.gene47666

ATGCGTCCACCGGTGAAGAGAGTTGATTACAAGGTAGGAGATAAAGTAGAAGTATGCAGCAAAGAAGAAGGGTTTGTAGGTTCATACTTTGAAGCTACAATTGTTTCATGTCTTGATAGTGGAAAATATGTGATCCGTTACAAGAATCTTCTTAAAGATGATGAGTCAGAACTTCTTATGGAAACGCTTTTTTCCAAAGATCTTCGTCCATTGCCTCCATGTGTTCGTAATCCTTCAAAGTTTGAACTCAATCAAAAAGTTGACGTGTTTGATAACGATGGTTGGTGGGTAGGGAAAATCGCTAGCGAGAAGATCTGGATGGAAAAGAGTTACTACTATAGTGTGTATTTTGACTATTGCCATCAAACTATTTACTACCCTTGTGATCAGATTAGGGTTCATCAAGAATTGGTTTGGGGAGACTGGATCTTCGAGGCATAA

Protein sequence

>MS.gene47666.t1

MRPPVKRVDYKVGDKVEVCSKEEGFVGSYFEATIVSCLDSGKYVIRYKNLLKDDESELLMETLFSKDLRPLPPCVRNPSKFELNQKVDVFDNDGWWVGKIASEKIWMEKSYYYSVYFDYCHQTIYYPCDQIRVHQELVWGDWIFEA