Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene48420.t1 | XP_003602916.2 | 94.6 | 480 | 26 | 0 | 1 | 480 | 1 | 480 | 6.30E-241 | 843.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene48420.t1 | G7J542 | 94.6 | 480 | 26 | 0 | 1 | 480 | 1 | 480 | 4.5e-241 | 843.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene48420.t1 | TR | PHD |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049142 | MS.gene48420 | 0.827113 | 1.87E-54 | -1.69E-46 |
| MS.gene052609 | MS.gene48420 | 0.805408 | 1.35E-49 | -1.69E-46 |
| MS.gene055313 | MS.gene48420 | 0.819822 | 9.49E-53 | -1.69E-46 |
| MS.gene055513 | MS.gene48420 | 0.810762 | 9.81E-51 | -1.69E-46 |
| MS.gene056181 | MS.gene48420 | 0.805486 | 1.30E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene48420.t1 | MTR_3g100350 | 97.083 | 480 | 14 | 0 | 1 | 480 | 1 | 480 | 0.0 | 966 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene48420.t1 | AT2G19260 | 47.297 | 222 | 90 | 5 | 261 | 480 | 410 | 606 | 1.60e-54 | 194 |
| MS.gene48420.t1 | AT2G19260 | 47.297 | 222 | 90 | 5 | 261 | 480 | 410 | 606 | 1.60e-54 | 194 |
Find 105 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTCTCCTGTATACCAAATT+AGG | 0.145112 | 3.1:-79800392 | None:intergenic |
| CTGCCTTCTGTCTTTCTTGA+TGG | 0.157712 | 3.1:+79800587 | MS.gene48420:CDS |
| GTCATTCATGTCTTAATAAA+AGG | 0.164007 | 3.1:+79801035 | MS.gene48420:CDS |
| TGAAAACACTGCACCTAATT+TGG | 0.214850 | 3.1:+79800379 | MS.gene48420:CDS |
| ATTTCAATTTGATGCTTAAC+TGG | 0.257269 | 3.1:-79800548 | None:intergenic |
| CCTCTTCCAATCCCGCCTTA+TGG | 0.261747 | 3.1:+79807146 | MS.gene48420:CDS |
| CTCTTCCAATCCCGCCTTAT+GGG | 0.272794 | 3.1:+79807147 | MS.gene48420:CDS |
| GGATTAATTTGCAATGGTTC+TGG | 0.279634 | 3.1:-79802139 | None:intergenic |
| CCACGTATCAACAGTGGAAT+AGG | 0.299158 | 3.1:+79801091 | MS.gene48420:CDS |
| TTCCTCTTCAAAGTCAAATA+TGG | 0.306991 | 3.1:+79800667 | MS.gene48420:CDS |
| GTCATTATGCTTGCAGTTGA+TGG | 0.308475 | 3.1:-79800356 | None:intergenic |
| GGGAAATTATATGTGGAAAA+TGG | 0.312495 | 3.1:+79802351 | MS.gene48420:CDS |
| ATCTCTCAACATCAACAGTA+TGG | 0.312758 | 3.1:-79801152 | None:intergenic |
| ACTTGTGAATGAAGTAAAAT+TGG | 0.312843 | 3.1:-79800480 | None:intergenic |
| ACTGGTCAGGCCCTGTAAAA+AGG | 0.314665 | 3.1:+79801233 | MS.gene48420:CDS |
| ATGCAAAATATGTGGTCATT+TGG | 0.356290 | 3.1:+79800910 | MS.gene48420:CDS |
| AACCAATGGAAGAGGATAAT+AGG | 0.372313 | 3.1:+79800285 | MS.gene48420:CDS |
| ACTCGCACACCAGTCGTATA+TGG | 0.376421 | 3.1:-79801181 | None:intergenic |
| GGAGTCCATTACTATTAAAC+TGG | 0.383396 | 3.1:-79800747 | None:intergenic |
| CCGTTAATAACTGCTGTTCC+AGG | 0.393854 | 3.1:+79800885 | MS.gene48420:CDS |
| AGAAGCCATGGACTTCTTAG+AGG | 0.397484 | 3.1:+79800815 | MS.gene48420:CDS |
| TTGAAGTTCAAACCGATGAC+TGG | 0.411899 | 3.1:+79805953 | MS.gene48420:CDS |
| CTCCTCCAGTTTAATAGTAA+TGG | 0.416267 | 3.1:+79800742 | MS.gene48420:CDS |
| GACTCAGTTCCATTGGCAAC+TGG | 0.419564 | 3.1:+79802297 | MS.gene48420:CDS |
| TGCTGATTGTGCTGTACCTC+AGG | 0.420947 | 3.1:+79806011 | MS.gene48420:CDS |
| CTTCTTAGTTCAGTTGAAGC+TGG | 0.423834 | 3.1:+79800143 | MS.gene48420:CDS |
| ATCTAGAACTGTCTCAGTTA+AGG | 0.430840 | 3.1:+79801117 | MS.gene48420:CDS |
| TGACAAAACCACTCATCAAT+TGG | 0.432889 | 3.1:-79801016 | None:intergenic |
| TTCCTATTATCCTCTTCCAT+TGG | 0.446285 | 3.1:-79800287 | None:intergenic |
| CTGCACCTAATTTGGTATAC+AGG | 0.447123 | 3.1:+79800387 | MS.gene48420:CDS |
| TGGCAACTGGCTTCAATGCC+GGG | 0.448378 | 3.1:+79802310 | MS.gene48420:CDS |
| GCCTAGTGGTGAAACTTGTC+AGG | 0.449374 | 3.1:+79800214 | MS.gene48420:CDS |
| GCAACTGAAGTATATAGAAA+TGG | 0.449691 | 3.1:+79806824 | MS.gene48420:CDS |
| TTCTGTTAATTCTTCCCTTG+TGG | 0.451346 | 3.1:-79800768 | None:intergenic |
| TTCAAGCAGCAGTCCTTGAC+TGG | 0.453183 | 3.1:+79801215 | MS.gene48420:CDS |
| CCAATGGAACTGAGTCTAGT+TGG | 0.456915 | 3.1:-79802290 | None:intergenic |
| TTGGCAACTGGCTTCAATGC+CGG | 0.457404 | 3.1:+79802309 | MS.gene48420:CDS |
| TAACTGGAAAAGCAGCTGTT+TGG | 0.459703 | 3.1:-79800532 | None:intergenic |
| GGTGGTAACGGCATCTTCAA+TGG | 0.462759 | 3.1:-79800864 | None:intergenic |
| ACGTGCCCATAAGGCGGGAT+TGG | 0.464010 | 3.1:-79807152 | None:intergenic |
| GGGAGGTTATAGACAAAACT+AGG | 0.470852 | 3.1:+79802330 | MS.gene48420:CDS |
| CTTAGACAGTTTGAGTTGAA+AGG | 0.472980 | 3.1:-79800320 | None:intergenic |
| AGAGATAAGACGTGCCCATA+AGG | 0.481151 | 3.1:-79807161 | None:intergenic |
| GCAGCAGTCCTTGACTGGTC+AGG | 0.487944 | 3.1:+79801220 | MS.gene48420:CDS |
| CACGTATCAACAGTGGAATA+GGG | 0.490240 | 3.1:+79801092 | MS.gene48420:CDS |
| AAGGGAAGAATTAACAGAAA+AGG | 0.490860 | 3.1:+79800772 | MS.gene48420:CDS |
| CCTGGAACAGCAGTTATTAA+CGG | 0.498635 | 3.1:-79800885 | None:intergenic |
| GTCATCCATCTCAGTTGCTA+TGG | 0.501394 | 3.1:-79800705 | None:intergenic |
| CTATGTTATGTATATGATGA+TGG | 0.506451 | 3.1:+79800167 | MS.gene48420:CDS |
| ACTGGTGTGCGAGTTGGTAA+AGG | 0.509532 | 3.1:+79801190 | MS.gene48420:CDS |
| AAGTTTCACCACTAGGCATC+CGG | 0.511152 | 3.1:-79800208 | None:intergenic |
| CCATAAGGCGGGATTGGAAG+AGG | 0.521585 | 3.1:-79807146 | None:intergenic |
| TCTGAAGGATTAATTTGCAA+TGG | 0.523395 | 3.1:-79802145 | None:intergenic |
| TACCATATTTGACTTTGAAG+AGG | 0.529766 | 3.1:-79800669 | None:intergenic |
| TTCAGAAATTTATAGACCGC+AGG | 0.529919 | 3.1:+79802162 | MS.gene48420:CDS |
| AAACGTGCATTGTAGCAAGA+TGG | 0.530372 | 3.1:-79800983 | None:intergenic |
| ATAAGACGTGCCCATAAGGC+GGG | 0.530589 | 3.1:-79807157 | None:intergenic |
| TCGGTTTGAACTTCAAATAG+TGG | 0.539950 | 3.1:-79805946 | None:intergenic |
| AGAAAGACAGAAGGCAGAGT+AGG | 0.541798 | 3.1:-79800581 | None:intergenic |
| AAAAGCTGAACCAATGGAAG+AGG | 0.542617 | 3.1:+79800277 | MS.gene48420:CDS |
| AGATTCCATAGCAACTGAGA+TGG | 0.550596 | 3.1:+79800700 | MS.gene48420:CDS |
| TTTGCACTTGTCATCAGCTG+AGG | 0.553862 | 3.1:+79800508 | MS.gene48420:CDS |
| CCAACTAGACTCAGTTCCAT+TGG | 0.554372 | 3.1:+79802290 | MS.gene48420:CDS |
| AAAACTAGGGAAATTATATG+TGG | 0.554778 | 3.1:+79802344 | MS.gene48420:CDS |
| TTACAGGGCCTGACCAGTCA+AGG | 0.559672 | 3.1:-79801228 | None:intergenic |
| GCACTTGTCTGCACATCGGT+CGG | 0.559798 | 3.1:-79800458 | None:intergenic |
| GCAACAAGTACCCTGCTGAC+TGG | 0.560642 | 3.1:+79800249 | MS.gene48420:CDS |
| TTATATGTGGAAAATGGCGC+AGG | 0.561470 | 3.1:+79802357 | MS.gene48420:CDS |
| TGCGGTCTATAAATTTCTGA+AGG | 0.561834 | 3.1:-79802160 | None:intergenic |
| CATTACTATTAAACTGGAGG+AGG | 0.567120 | 3.1:-79800741 | None:intergenic |
| TGAAGTTCAAACCGATGACT+GGG | 0.570865 | 3.1:+79805954 | MS.gene48420:CDS |
| GCAGTTATTAACGGTGGTAA+CGG | 0.572035 | 3.1:-79800876 | None:intergenic |
| GTGTAGAAAAGCTGAACCAA+TGG | 0.583872 | 3.1:+79800271 | MS.gene48420:CDS |
| CCTATTCCACTGTTGATACG+TGG | 0.585601 | 3.1:-79801091 | None:intergenic |
| GCATTGAAGCCAGTTGCCAA+TGG | 0.585878 | 3.1:-79802306 | None:intergenic |
| TCTCTCAACATCAACAGTAT+GGG | 0.587204 | 3.1:-79801151 | None:intergenic |
| ACAGAAGCACTCCCAGTCAT+CGG | 0.588116 | 3.1:-79805965 | None:intergenic |
| TAATAGTAATGGACTCCACA+AGG | 0.589290 | 3.1:+79800753 | MS.gene48420:CDS |
| TATACGACTGGTGTGCGAGT+TGG | 0.590103 | 3.1:+79801184 | MS.gene48420:CDS |
| TATGTTATGTATATGATGAT+GGG | 0.592983 | 3.1:+79800168 | MS.gene48420:CDS |
| TCCAGGTCATGCAAAATATG+TGG | 0.597837 | 3.1:+79800902 | MS.gene48420:CDS |
| AGATACTCTTACAGATAATG+TGG | 0.604361 | 3.1:+79800838 | MS.gene48420:CDS |
| GTCCATTACTATTAAACTGG+AGG | 0.605303 | 3.1:-79800744 | None:intergenic |
| ACAGCACAATCAGCATGAGA+TGG | 0.605642 | 3.1:-79806003 | None:intergenic |
| TATCTCCTCTAAGAAGTCCA+TGG | 0.605795 | 3.1:-79800820 | None:intergenic |
| AAAGAGTACCAATTGATGAG+TGG | 0.612414 | 3.1:+79801008 | MS.gene48420:CDS |
| ATTCCACTGTTGATACGTGG+TGG | 0.618449 | 3.1:-79801088 | None:intergenic |
| CAACGTGACCGGATGCCTAG+TGG | 0.622842 | 3.1:+79800200 | MS.gene48420:CDS |
| ACCTGACAAGTTTCACCACT+AGG | 0.624843 | 3.1:-79800215 | None:intergenic |
| GATAAGACGTGCCCATAAGG+CGG | 0.625500 | 3.1:-79807158 | None:intergenic |
| TCAAACTGTCTAAGAAGCTG+TGG | 0.626076 | 3.1:+79800329 | MS.gene48420:CDS |
| GATACTGAGCCATATACGAC+TGG | 0.630007 | 3.1:+79801172 | MS.gene48420:CDS |
| AGCTGATGACAAGTGCAAAG+AGG | 0.633110 | 3.1:-79800504 | None:intergenic |
| AAGGCGGGATTGGAAGAGGA+GGG | 0.636500 | 3.1:-79807142 | None:intergenic |
| TAAGGCGGGATTGGAAGAGG+AGG | 0.641496 | 3.1:-79807143 | None:intergenic |
| GGAGGTTATAGACAAAACTA+GGG | 0.658232 | 3.1:+79802331 | MS.gene48420:CDS |
| GCAGGAGGTGGAAACAGATG+AGG | 0.662977 | 3.1:+79806794 | MS.gene48420:intron |
| ACTCCATCAAGAAAGACAGA+AGG | 0.671873 | 3.1:-79800590 | None:intergenic |
| AAACCACCACGTATCAACAG+TGG | 0.676321 | 3.1:+79801085 | MS.gene48420:CDS |
| AATAGTAATGGACTCCACAA+GGG | 0.679473 | 3.1:+79800754 | MS.gene48420:CDS |
| ATTGGCACTTGTCTGCACAT+CGG | 0.680439 | 3.1:-79800462 | None:intergenic |
| AGGCGGGATTGGAAGAGGAG+GGG | 0.692067 | 3.1:-79807141 | None:intergenic |
| CAACTGGCTTCAATGCCGGG+AGG | 0.715328 | 3.1:+79802313 | MS.gene48420:CDS |
| GCAACTGAGATGGATGACAC+TGG | 0.735945 | 3.1:+79800710 | MS.gene48420:CDS |
| GGAACAGCAGTTATTAACGG+TGG | 0.741703 | 3.1:-79800882 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 79800125 | 79807198 | 79800125 | ID=MS.gene48420 |
| chr3.1 | mRNA | 79800125 | 79807198 | 79800125 | ID=MS.gene48420.t1;Parent=MS.gene48420 |
| chr3.1 | exon | 79800125 | 79801254 | 79800125 | ID=MS.gene48420.t1.exon1;Parent=MS.gene48420.t1 |
| chr3.1 | CDS | 79800125 | 79801254 | 79800125 | ID=cds.MS.gene48420.t1;Parent=MS.gene48420.t1 |
| chr3.1 | exon | 79802123 | 79802183 | 79802123 | ID=MS.gene48420.t1.exon2;Parent=MS.gene48420.t1 |
| chr3.1 | CDS | 79802123 | 79802183 | 79802123 | ID=cds.MS.gene48420.t1;Parent=MS.gene48420.t1 |
| chr3.1 | exon | 79802272 | 79802378 | 79802272 | ID=MS.gene48420.t1.exon3;Parent=MS.gene48420.t1 |
| chr3.1 | CDS | 79802272 | 79802378 | 79802272 | ID=cds.MS.gene48420.t1;Parent=MS.gene48420.t1 |
| chr3.1 | exon | 79805942 | 79806032 | 79805942 | ID=MS.gene48420.t1.exon4;Parent=MS.gene48420.t1 |
| chr3.1 | CDS | 79805942 | 79806032 | 79805942 | ID=cds.MS.gene48420.t1;Parent=MS.gene48420.t1 |
| chr3.1 | exon | 79806798 | 79806845 | 79806798 | ID=MS.gene48420.t1.exon5;Parent=MS.gene48420.t1 |
| chr3.1 | CDS | 79806798 | 79806845 | 79806798 | ID=cds.MS.gene48420.t1;Parent=MS.gene48420.t1 |
| chr3.1 | exon | 79807130 | 79807198 | 79807130 | ID=MS.gene48420.t1.exon6;Parent=MS.gene48420.t1 |
| chr3.1 | CDS | 79807130 | 79807198 | 79807130 | ID=cds.MS.gene48420.t1;Parent=MS.gene48420.t1 |
| Gene Sequence |
| Protein sequence |
