Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050611 | MS.gene48750 | 0.867063 | 1.80E-65 | -1.69E-46 |
| MS.gene058294 | MS.gene48750 | 0.812037 | 5.19E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene48750.t1 | MTR_8g432580 | 86.486 | 74 | 10 | 0 | 8 | 81 | 52 | 125 | 2.65e-24 | 91.3 |
| MS.gene48750.t1 | MTR_4g074930 | 86.420 | 81 | 10 | 1 | 2 | 82 | 26 | 105 | 3.88e-23 | 93.6 |
| MS.gene48750.t1 | MTR_4g074930 | 86.420 | 81 | 10 | 1 | 2 | 82 | 26 | 105 | 4.06e-23 | 93.6 |
| MS.gene48750.t1 | MTR_4g074930 | 86.420 | 81 | 10 | 1 | 2 | 82 | 26 | 105 | 4.33e-23 | 93.2 |
| MS.gene48750.t1 | MTR_4g074930 | 86.420 | 81 | 10 | 1 | 2 | 82 | 26 | 105 | 4.66e-23 | 93.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 34 sgRNAs with CRISPR-Local
Find 83 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCTCTGAAGTGGTCTCTGGC+TGG | 0.335134 | 4.4:-72898755 | None:intergenic |
| CAAGAAGGAGGAAAGTAGTT+TGG | 0.335928 | 4.4:+72898517 | MS.gene48750:CDS |
| TTAGTTGGGGTCTTCTTTGA+AGG | 0.356776 | 4.4:-72898711 | None:intergenic |
| CTCTGAAGTGGTCTCTGGCT+GGG | 0.379329 | 4.4:-72898754 | None:intergenic |
| GATTCCTCTGAAGTGGTCTC+TGG | 0.415465 | 4.4:-72898759 | None:intergenic |
| CACTTCTTCCTCATCGGAAG+AGG | 0.423585 | 4.4:-72898799 | None:intergenic |
| CGCCAAGCAAAAGGCGTTGA+AGG | 0.441849 | 4.4:+72898490 | MS.gene48750:CDS |
| GGGGTCTTCTTTGAAGGAGC+AGG | 0.444374 | 4.4:-72898705 | None:intergenic |
| CACTTTCACTTCTTCCTCAT+CGG | 0.467568 | 4.4:-72898805 | None:intergenic |
| AGAGGTTGCCGCCAAGCAAA+AGG | 0.479123 | 4.4:+72898481 | MS.gene48750:CDS |
| CTCTGAGGAATCTGAGGATG+AGG | 0.511350 | 4.4:+72898547 | MS.gene48750:CDS |
| GAAGGAGGAAAGTAGTTTGG+AGG | 0.514144 | 4.4:+72898520 | MS.gene48750:CDS |
| TTTCTCGGAAAGATGAAGCT+TGG | 0.526996 | 4.4:+72898146 | None:intergenic |
| GGCGTTGAAGGTAGTCAAGA+AGG | 0.528027 | 4.4:+72898502 | MS.gene48750:CDS |
| GACTTCGACTTCGCCGATGC+CGG | 0.528326 | 4.4:+72899261 | MS.gene48750:CDS |
| CAGTCAGTGCAAAGAAGCAA+GGG | 0.534511 | 4.4:+72898453 | MS.gene48750:CDS |
| AATTGGATTATCGCCGGCAT+CGG | 0.534727 | 4.4:-72899274 | None:intergenic |
| CTTTGATATAGGCAAGAGGC+AGG | 0.543584 | 4.4:+72898412 | MS.gene48750:intron |
| GCAGGCTGAGGAAGAAGTCA+AGG | 0.547712 | 4.4:+72898430 | MS.gene48750:CDS |
| AGAGTCAGATTCCTCTGAAG+TGG | 0.548311 | 4.4:-72898766 | None:intergenic |
| GCAGTCAGTGCAAAGAAGCA+AGG | 0.565779 | 4.4:+72898452 | MS.gene48750:CDS |
| CTTGAGAATTGGATTATCGC+CGG | 0.569266 | 4.4:-72899280 | None:intergenic |
| GGAATCCTCTGAGGAATCTG+AGG | 0.584932 | 4.4:+72898541 | MS.gene48750:CDS |
| TATAGGCAAGAGGCAGGCTG+AGG | 0.588543 | 4.4:+72898418 | MS.gene48750:intron |
| AAAGAAGCAAGGGGTAGAAG+AGG | 0.598623 | 4.4:+72898463 | MS.gene48750:CDS |
| CGGCGATAATCCAATTCTCA+AGG | 0.616014 | 4.4:+72899281 | MS.gene48750:CDS |
| CCAGCCAGAGACCACTTCAG+AGG | 0.625597 | 4.4:+72898755 | MS.gene48750:CDS |
| TTCACTTTGATATAGGCAAG+AGG | 0.629817 | 4.4:+72898408 | MS.gene48750:intron |
| TGACAGCTCCTCTTCCGATG+AGG | 0.667005 | 4.4:+72898791 | MS.gene48750:CDS |
| AGTCAGTGCAAAGAAGCAAG+GGG | 0.679652 | 4.4:+72898454 | MS.gene48750:CDS |
| GTTGAAGGTAGTCAAGAAGG+AGG | 0.683060 | 4.4:+72898505 | MS.gene48750:CDS |
| CTCATCCTCAGATTCCTCAG+AGG | 0.686828 | 4.4:-72898546 | None:intergenic |
| GATAATCCAATTCTCAAGGT+CGG | 0.697498 | 4.4:+72899285 | MS.gene48750:CDS |
| TAGTTTGGAGGAATCCTCTG+AGG | 0.723183 | 4.4:+72898532 | MS.gene48750:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATGTTTAAGATATAAATCAA+AGG | - | chr4.4:72898878-72898897 | None:intergenic | 15.0% |
| !!! | GTTTTAAAAAATAGAACAAT+AGG | - | chr4.4:72898969-72898988 | None:intergenic | 15.0% |
| !! | AGAACATCAAATAATATTGA+AGG | - | chr4.4:72898269-72898288 | None:intergenic | 20.0% |
| !!! | ATTTTTTTATGTTCTGATGT+CGG | + | chr4.4:72898367-72898386 | MS.gene48750:intron | 20.0% |
| !!! | GTGTAATTATTTTTTTGTTG+CGG | + | chr4.4:72899170-72899189 | MS.gene48750:intron | 20.0% |
| !!! | TCTATAATTCACTTTTTAGA+AGG | + | chr4.4:72898992-72899011 | MS.gene48750:intron | 20.0% |
| !!! | TTTTCTGATGTTGATTATAT+AGG | + | chr4.4:72898205-72898224 | MS.gene48750:intron | 20.0% |
| ! | ATAATAACATCAGACCAAAT+TGG | - | chr4.4:72898179-72898198 | None:intergenic | 25.0% |
| ! | TAATAACATCAGACCAAATT+GGG | - | chr4.4:72898178-72898197 | None:intergenic | 25.0% |
| ! | TTATATCTTAAACACTGTGT+TGG | + | chr4.4:72898940-72898959 | MS.gene48750:intron | 25.0% |
| !! | TATGTCATGTTAATTTTACG+TGG | + | chr4.4:72898658-72898677 | MS.gene48750:intron | 25.0% |
| !!! | CGGTTTATATTTTTTTGTTG+CGG | + | chr4.4:72899190-72899209 | MS.gene48750:intron | 25.0% |
| !!! | CTTCTAGATTTTGGTTAATT+TGG | - | chr4.4:72898237-72898256 | None:intergenic | 25.0% |
| AATAACATCAGACCAAATTG+GGG | - | chr4.4:72898177-72898196 | None:intergenic | 30.0% | |
| GTAAAATTTCGATATTCCTG+CGG | + | chr4.4:72899221-72899240 | MS.gene48750:intron | 30.0% | |
| TTAACCAAAATCTAGAAGCT+TGG | + | chr4.4:72898239-72898258 | MS.gene48750:intron | 30.0% | |
| !! | GGCTATTTTCACTTTGATAT+AGG | + | chr4.4:72898401-72898420 | MS.gene48750:intron | 30.0% |
| AAAAAGGAAATCGGAAAGCA+AGG | + | chr4.4:72899312-72899331 | MS.gene48750:CDS | 35.0% | |
| AAGAAGACCCCAACTAAAAA+TGG | + | chr4.4:72898717-72898736 | MS.gene48750:CDS | 35.0% | |
| AAGTGTGAGCAACAAAATAG+TGG | - | chr4.4:72899032-72899051 | None:intergenic | 35.0% | |
| AGAAGACCCCAACTAAAAAT+GGG | + | chr4.4:72898718-72898737 | MS.gene48750:CDS | 35.0% | |
| ATAACATCAGACCAAATTGG+GGG | - | chr4.4:72898176-72898195 | None:intergenic | 35.0% | |
| ATGCTTTAACGCATTTCGAA+TGG | + | chr4.4:72898588-72898607 | MS.gene48750:intron | 35.0% | |
| GATAATCCAATTCTCAAGGT+CGG | + | chr4.4:72899285-72899304 | MS.gene48750:CDS | 35.0% | |
| GTCGGTGAAAAAAAGGAAAT+CGG | + | chr4.4:72899303-72899322 | MS.gene48750:CDS | 35.0% | |
| TTCACTTTGATATAGGCAAG+AGG | + | chr4.4:72898408-72898427 | MS.gene48750:intron | 35.0% | |
| ! | AGAACCAAGCTTCTAGATTT+TGG | - | chr4.4:72898246-72898265 | None:intergenic | 35.0% |
| ! | TGTTGAAGTGTTTACCAGTT+AGG | - | chr4.4:72899147-72899166 | None:intergenic | 35.0% |
| ! | TTTTGCGTTGGAAACAAAAC+AGG | - | chr4.4:72899068-72899087 | None:intergenic | 35.0% |
| !!! | CTGATGTCGGTTTTCTTATT+GGG | + | chr4.4:72898380-72898399 | MS.gene48750:intron | 35.0% |
| !!! | TCACACTCCCATTTTTAGTT+GGG | - | chr4.4:72898728-72898747 | None:intergenic | 35.0% |
| !!! | TCTGATGTCGGTTTTCTTAT+TGG | + | chr4.4:72898379-72898398 | MS.gene48750:intron | 35.0% |
| !!! | TTCACACTCCCATTTTTAGT+TGG | - | chr4.4:72898729-72898748 | None:intergenic | 35.0% |
| ATAGGTCAAGGATAGGAGAA+AGG | - | chr4.4:72899093-72899112 | None:intergenic | 40.0% | |
| CAAGAAGGAGGAAAGTAGTT+TGG | + | chr4.4:72898517-72898536 | MS.gene48750:CDS | 40.0% | |
| CACTTTCACTTCTTCCTCAT+CGG | - | chr4.4:72898808-72898827 | None:intergenic | 40.0% | |
| CTTGAGAATTGGATTATCGC+CGG | - | chr4.4:72899283-72899302 | None:intergenic | 40.0% | |
| TCTCAAGGTCGGTGAAAAAA+AGG | + | chr4.4:72899296-72899315 | MS.gene48750:CDS | 40.0% | |
| TTAGTTGGGGTCTTCTTTGA+AGG | - | chr4.4:72898714-72898733 | None:intergenic | 40.0% | |
| TTTACCAGTTAGGCAGTGAT+AGG | - | chr4.4:72899137-72899156 | None:intergenic | 40.0% | |
| TTTCTCCTATCCTTGACCTA+TGG | + | chr4.4:72899092-72899111 | MS.gene48750:intron | 40.0% | |
| ! | AGGAGAAAGGATTTTTGCGT+TGG | - | chr4.4:72899080-72899099 | None:intergenic | 40.0% |
| ! | TTTTCACCGACCTTGAGAAT+TGG | - | chr4.4:72899294-72899313 | None:intergenic | 40.0% |
| !!! | CACACTCCCATTTTTAGTTG+GGG | - | chr4.4:72898727-72898746 | None:intergenic | 40.0% |
| AAAGAAGCAAGGGGTAGAAG+AGG | + | chr4.4:72898463-72898482 | MS.gene48750:CDS | 45.0% | |
| AATTGGATTATCGCCGGCAT+CGG | - | chr4.4:72899277-72899296 | None:intergenic | 45.0% | |
| AGTCAGTGCAAAGAAGCAAG+GGG | + | chr4.4:72898454-72898473 | MS.gene48750:CDS | 45.0% | |
| CAGTCAGTGCAAAGAAGCAA+GGG | + | chr4.4:72898453-72898472 | MS.gene48750:CDS | 45.0% | |
| CGGCGATAATCCAATTCTCA+AGG | + | chr4.4:72899281-72899300 | MS.gene48750:CDS | 45.0% | |
| CTTTGATATAGGCAAGAGGC+AGG | + | chr4.4:72898412-72898431 | MS.gene48750:intron | 45.0% | |
| GAAGGAGGAAAGTAGTTTGG+AGG | + | chr4.4:72898520-72898539 | MS.gene48750:CDS | 45.0% | |
| GAAGTCGAAGTCGTCTTTCA+TGG | - | chr4.4:72899253-72899272 | None:intergenic | 45.0% | |
| GTTGAAGGTAGTCAAGAAGG+AGG | + | chr4.4:72898505-72898524 | MS.gene48750:CDS | 45.0% | |
| TAGTTTGGAGGAATCCTCTG+AGG | + | chr4.4:72898532-72898551 | MS.gene48750:CDS | 45.0% | |
| TCTTTCATGGAATCAACCGC+AGG | - | chr4.4:72899240-72899259 | None:intergenic | 45.0% | |
| TTTCTTCCAGCCATAGGTCA+AGG | - | chr4.4:72899105-72899124 | None:intergenic | 45.0% | |
| ! | AGAGTCAGATTCCTCTGAAG+TGG | - | chr4.4:72898769-72898788 | None:intergenic | 45.0% |
| ! | TACCTTCAACGCCTTTTGCT+TGG | - | chr4.4:72898495-72898514 | None:intergenic | 45.0% |
| CACTTCTTCCTCATCGGAAG+AGG | - | chr4.4:72898802-72898821 | None:intergenic | 50.0% | |
| CAGTTAGGCAGTGATAGGAG+AGG | - | chr4.4:72899132-72899151 | None:intergenic | 50.0% | |
| CTCATCCTCAGATTCCTCAG+AGG | - | chr4.4:72898549-72898568 | None:intergenic | 50.0% | |
| CTCTCCTATCACTGCCTAAC+TGG | + | chr4.4:72899130-72899149 | MS.gene48750:intron | 50.0% | |
| GCAGTCAGTGCAAAGAAGCA+AGG | + | chr4.4:72898452-72898471 | MS.gene48750:CDS | 50.0% | |
| GGAGAGTTTCTTCCAGCCAT+AGG | - | chr4.4:72899111-72899130 | None:intergenic | 50.0% | |
| TCCAGCCATAGGTCAAGGAT+AGG | - | chr4.4:72899100-72899119 | None:intergenic | 50.0% | |
| TCCTATCCTTGACCTATGGC+TGG | + | chr4.4:72899096-72899115 | MS.gene48750:intron | 50.0% | |
| ! | AGCTTGGTTCTCCCCCAATT+TGG | + | chr4.4:72898162-72898181 | MS.gene48750:intron | 50.0% |
| ! | CTCTGAGGAATCTGAGGATG+AGG | + | chr4.4:72898547-72898566 | MS.gene48750:CDS | 50.0% |
| ! | CTTCAACGCCTTTTGCTTGG+CGG | - | chr4.4:72898492-72898511 | None:intergenic | 50.0% |
| ! | GGAATCCTCTGAGGAATCTG+AGG | + | chr4.4:72898541-72898560 | MS.gene48750:CDS | 50.0% |
| ! | GGCGTTGAAGGTAGTCAAGA+AGG | + | chr4.4:72898502-72898521 | MS.gene48750:CDS | 50.0% |
| !! | GATTCCTCTGAAGTGGTCTC+TGG | - | chr4.4:72898762-72898781 | None:intergenic | 50.0% |
| AGAGGTTGCCGCCAAGCAAA+AGG | + | chr4.4:72898481-72898500 | MS.gene48750:CDS | 55.0% | |
| GCAGGCTGAGGAAGAAGTCA+AGG | + | chr4.4:72898430-72898449 | MS.gene48750:CDS | 55.0% | |
| TATAGGCAAGAGGCAGGCTG+AGG | + | chr4.4:72898418-72898437 | MS.gene48750:intron | 55.0% | |
| TGACAGCTCCTCTTCCGATG+AGG | + | chr4.4:72898791-72898810 | MS.gene48750:CDS | 55.0% | |
| ! | GGAGCAGGAGCTTTTGCAAC+AGG | - | chr4.4:72898693-72898712 | None:intergenic | 55.0% |
| ! | GGGGTCTTCTTTGAAGGAGC+AGG | - | chr4.4:72898708-72898727 | None:intergenic | 55.0% |
| !! | CGCCAAGCAAAAGGCGTTGA+AGG | + | chr4.4:72898490-72898509 | MS.gene48750:CDS | 55.0% |
| !! | CTCTGAAGTGGTCTCTGGCT+GGG | - | chr4.4:72898757-72898776 | None:intergenic | 55.0% |
| CCAGCCAGAGACCACTTCAG+AGG | + | chr4.4:72898755-72898774 | MS.gene48750:CDS | 60.0% | |
| GACTTCGACTTCGCCGATGC+CGG | + | chr4.4:72899261-72899280 | MS.gene48750:CDS | 60.0% | |
| !! | CCTCTGAAGTGGTCTCTGGC+TGG | - | chr4.4:72898758-72898777 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 72898158 | 72899339 | 72898158 | ID=MS.gene48750 |
| chr4.4 | mRNA | 72898158 | 72899339 | 72898158 | ID=MS.gene48750.t1;Parent=MS.gene48750 |
| chr4.4 | exon | 72898158 | 72898167 | 72898158 | ID=MS.gene48750.t1.exon1;Parent=MS.gene48750.t1 |
| chr4.4 | CDS | 72898158 | 72898167 | 72898158 | ID=cds.MS.gene48750.t1;Parent=MS.gene48750.t1 |
| chr4.4 | exon | 72898423 | 72898568 | 72898423 | ID=MS.gene48750.t1.exon2;Parent=MS.gene48750.t1 |
| chr4.4 | CDS | 72898423 | 72898568 | 72898423 | ID=cds.MS.gene48750.t1;Parent=MS.gene48750.t1 |
| chr4.4 | exon | 72898687 | 72898824 | 72898687 | ID=MS.gene48750.t1.exon3;Parent=MS.gene48750.t1 |
| chr4.4 | CDS | 72898687 | 72898824 | 72898687 | ID=cds.MS.gene48750.t1;Parent=MS.gene48750.t1 |
| chr4.4 | exon | 72899259 | 72899339 | 72899259 | ID=MS.gene48750.t1.exon4;Parent=MS.gene48750.t1 |
| chr4.4 | CDS | 72899259 | 72899339 | 72899259 | ID=cds.MS.gene48750.t1;Parent=MS.gene48750.t1 |
| Gene Sequence |
| Protein sequence |
