Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050611 | MS.gene055818 | 0.800141 | 1.66E-48 | -1.69E-46 |
| MS.gene050611 | MS.gene061942 | 0.810222 | 1.28E-50 | -1.69E-46 |
| MS.gene050611 | MS.gene06269 | 0.865171 | 7.16E-65 | -1.69E-46 |
| MS.gene050611 | MS.gene063357 | 0.80116 | 1.03E-48 | -1.69E-46 |
| MS.gene050611 | MS.gene063846 | 0.803429 | 3.50E-49 | -1.69E-46 |
| MS.gene050611 | MS.gene066752 | 0.818527 | 1.87E-52 | -1.69E-46 |
| MS.gene050611 | MS.gene067656 | 0.8173 | 3.54E-52 | -1.69E-46 |
| MS.gene050611 | MS.gene24211 | 0.801751 | 7.77E-49 | -1.69E-46 |
| MS.gene050611 | MS.gene24885 | 0.815177 | 1.06E-51 | -1.69E-46 |
| MS.gene050611 | MS.gene25778 | 0.815241 | 1.02E-51 | -1.69E-46 |
| MS.gene050611 | MS.gene26056 | 0.84572 | 3.40E-59 | -1.69E-46 |
| MS.gene050611 | MS.gene31142 | 0.801254 | 9.82E-49 | -1.69E-46 |
| MS.gene050611 | MS.gene31283 | 0.804645 | 1.96E-49 | -1.69E-46 |
| MS.gene050611 | MS.gene32262 | 0.817799 | 2.73E-52 | -1.69E-46 |
| MS.gene050611 | MS.gene32747 | 0.811079 | 8.38E-51 | -1.69E-46 |
| MS.gene050611 | MS.gene38134 | 0.811886 | 5.59E-51 | -1.69E-46 |
| MS.gene050611 | MS.gene38429 | 0.822549 | 2.23E-53 | -1.69E-46 |
| MS.gene050611 | MS.gene38876 | 0.807639 | 4.58E-50 | -1.69E-46 |
| MS.gene050611 | MS.gene42645 | 0.814157 | 1.78E-51 | -1.69E-46 |
| MS.gene050611 | MS.gene45794 | 0.805825 | 1.11E-49 | -1.69E-46 |
| MS.gene050611 | MS.gene47167 | 0.838833 | 2.27E-57 | -1.69E-46 |
| MS.gene050611 | MS.gene48750 | 0.867063 | 1.80E-65 | -1.69E-46 |
| MS.gene050611 | MS.gene53501 | 0.800429 | 1.45E-48 | -1.69E-46 |
| MS.gene050611 | MS.gene54842 | 0.818328 | 2.08E-52 | -1.69E-46 |
| MS.gene050611 | MS.gene59780 | 0.82774 | 1.32E-54 | -1.69E-46 |
| MS.gene050611 | MS.gene62748 | 0.802197 | 6.29E-49 | -1.69E-46 |
| MS.gene050611 | MS.gene64249 | 0.824445 | 8.04E-54 | -1.69E-46 |
| MS.gene050611 | MS.gene65816 | 0.825897 | 3.65E-54 | -1.69E-46 |
| MS.gene050611 | MS.gene67266 | 0.821733 | 3.45E-53 | -1.69E-46 |
| MS.gene050611 | MS.gene69594 | 0.806715 | 7.19E-50 | -1.69E-46 |
| MS.gene050611 | MS.gene72205 | 0.827742 | 1.32E-54 | -1.69E-46 |
| MS.gene050611 | MS.gene72268 | 0.818238 | 2.18E-52 | -1.69E-46 |
| MS.gene050611 | MS.gene73540 | 0.800099 | 1.69E-48 | -1.69E-46 |
| MS.gene050611 | MS.gene76612 | 0.806189 | 9.28E-50 | -1.69E-46 |
| MS.gene050611 | MS.gene78647 | 0.827002 | 1.99E-54 | -1.69E-46 |
| MS.gene050611 | MS.gene78775 | 0.806875 | 6.65E-50 | -1.69E-46 |
| MS.gene050611 | MS.gene79147 | 0.800658 | 1.30E-48 | -1.69E-46 |
| MS.gene050611 | MS.gene82663 | 0.81487 | 1.24E-51 | -1.69E-46 |
| MS.gene050611 | MS.gene82668 | 0.807346 | 5.29E-50 | -1.69E-46 |
| MS.gene050611 | MS.gene82669 | 0.807405 | 5.14E-50 | -1.69E-46 |
| MS.gene050611 | MS.gene82682 | 0.803587 | 3.25E-49 | -1.69E-46 |
| MS.gene050611 | MS.gene82962 | 0.816175 | 6.33E-52 | -1.69E-46 |
| MS.gene050611 | MS.gene84968 | 0.806264 | 8.95E-50 | -1.69E-46 |
| MS.gene050611 | MS.gene85436 | 0.813416 | 2.59E-51 | -1.69E-46 |
| MS.gene050611 | MS.gene88527 | 0.801133 | 1.04E-48 | -1.69E-46 |
| MS.gene050611 | MS.gene92383 | 0.810627 | 1.05E-50 | -1.69E-46 |
| MS.gene050611 | MS.gene92642 | 0.801365 | 9.32E-49 | -1.69E-46 |
| MS.gene050611 | MS.gene95427 | 0.807332 | 5.32E-50 | -1.69E-46 |
| MS.gene050611 | MS.gene98550 | 0.801586 | 8.40E-49 | -1.69E-46 |
| MS.gene050611 | MS.gene99751 | 0.803121 | 4.06E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene050611.t1 | MTR_8g432580 | 87.838 | 74 | 9 | 0 | 8 | 81 | 52 | 125 | 3.71e-25 | 92.8 |
| MS.gene050611.t1 | MTR_4g074930 | 86.842 | 76 | 9 | 1 | 2 | 77 | 26 | 100 | 1.80e-24 | 96.7 |
| MS.gene050611.t1 | MTR_4g074930 | 86.842 | 76 | 9 | 1 | 2 | 77 | 26 | 100 | 1.93e-24 | 96.7 |
| MS.gene050611.t1 | MTR_4g074930 | 86.842 | 76 | 9 | 1 | 2 | 77 | 26 | 100 | 2.26e-24 | 96.3 |
| MS.gene050611.t1 | MTR_4g074930 | 86.842 | 76 | 9 | 1 | 2 | 77 | 26 | 100 | 2.53e-24 | 96.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 31 sgRNAs with CRISPR-Local
Find 76 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCTCTGAAGTGGTCTCTGGC+TGG | 0.335134 | 7.3:-1327819 | None:intergenic |
| CAAGAAGGAGGAAAGTAGTT+TGG | 0.335928 | 7.3:+1327581 | MS.gene050611:CDS |
| TTAGTTGGGGTCTTCTTTGA+AGG | 0.356776 | 7.3:-1327775 | None:intergenic |
| CTCTGAAGTGGTCTCTGGCT+GGG | 0.379329 | 7.3:-1327818 | None:intergenic |
| GATTCCTCTGAAGTGGTCTC+TGG | 0.415465 | 7.3:-1327823 | None:intergenic |
| CACTTCTTCCTCATCGGAAG+AGG | 0.423585 | 7.3:-1327863 | None:intergenic |
| CGCCAAGCAAAAGGCGTTGA+AGG | 0.441849 | 7.3:+1327554 | MS.gene050611:CDS |
| GGGGTCTTCTTTGAAGGAGC+AGG | 0.444374 | 7.3:-1327769 | None:intergenic |
| CACTTTCACTTCTTCCTCAT+CGG | 0.467568 | 7.3:-1327869 | None:intergenic |
| AGAGGTTGCCGCCAAGCAAA+AGG | 0.479123 | 7.3:+1327545 | MS.gene050611:CDS |
| TTTCTCGGAAAGATGAAGCT+TGG | 0.505304 | 7.3:+1327209 | None:intergenic |
| CTCTGAGGAATCTGAGGATG+AGG | 0.511350 | 7.3:+1327611 | MS.gene050611:CDS |
| GAAGGAGGAAAGTAGTTTGG+AGG | 0.514144 | 7.3:+1327584 | MS.gene050611:CDS |
| GGCGTTGAAGGTAGTCAAGA+AGG | 0.528027 | 7.3:+1327566 | MS.gene050611:CDS |
| AATGAAATTAAAAGCATGGT+AGG | 0.528809 | 7.3:+1328434 | MS.gene050611:CDS |
| AAAGTCAGATTCCTCTGAAG+TGG | 0.537679 | 7.3:-1327830 | None:intergenic |
| CAGTGAGTGCAAAGAAGCAA+GGG | 0.541082 | 7.3:+1327517 | MS.gene050611:CDS |
| CTTTGATATAGGCAAGAGGC+AGG | 0.543584 | 7.3:+1327476 | MS.gene050611:intron |
| GCAGGCTGAGGAAGAAGTCA+AGG | 0.547712 | 7.3:+1327494 | MS.gene050611:CDS |
| GTCAAATGAAATTAAAAGCA+TGG | 0.549577 | 7.3:+1328430 | MS.gene050611:CDS |
| GCAGTGAGTGCAAAGAAGCA+AGG | 0.558038 | 7.3:+1327516 | MS.gene050611:CDS |
| GGAATCCTCTGAGGAATCTG+AGG | 0.584932 | 7.3:+1327605 | MS.gene050611:CDS |
| TATAGGCAAGAGGCAGGCTG+AGG | 0.588543 | 7.3:+1327482 | MS.gene050611:intron |
| AAAGAAGCAAGGGGTAGAAG+AGG | 0.598623 | 7.3:+1327527 | MS.gene050611:CDS |
| CCAGCCAGAGACCACTTCAG+AGG | 0.625597 | 7.3:+1327819 | MS.gene050611:CDS |
| TTCACTTTGATATAGGCAAG+AGG | 0.629817 | 7.3:+1327472 | MS.gene050611:intron |
| TGACAGCTCCTCTTCCGATG+AGG | 0.667005 | 7.3:+1327855 | MS.gene050611:CDS |
| AGTGAGTGCAAAGAAGCAAG+GGG | 0.679652 | 7.3:+1327518 | MS.gene050611:CDS |
| GTTGAAGGTAGTCAAGAAGG+AGG | 0.683060 | 7.3:+1327569 | MS.gene050611:CDS |
| CTCATCCTCAGATTCCTCAG+AGG | 0.686828 | 7.3:-1327610 | None:intergenic |
| TAGTTTGGAGGAATCCTCTG+AGG | 0.723183 | 7.3:+1327596 | MS.gene050611:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATGTTTAAGATATAAATCAA+AGG | - | chr7.3:1327942-1327961 | None:intergenic | 15.0% |
| !! | AGAACATCAAATAATATTGA+AGG | - | chr7.3:1327332-1327351 | None:intergenic | 20.0% |
| !! | ATAATAACATCAGACAAAAT+TGG | - | chr7.3:1327242-1327261 | None:intergenic | 20.0% |
| !! | TAATAACATCAGACAAAATT+GGG | - | chr7.3:1327241-1327260 | None:intergenic | 20.0% |
| !!! | ATTTTTTTATGTTCTGATGT+CGG | + | chr7.3:1327431-1327450 | MS.gene050611:intron | 20.0% |
| !!! | GTTTTAAAAAACAGAACAAT+AGG | - | chr7.3:1328033-1328052 | None:intergenic | 20.0% |
| !!! | TCTATAATTCACTTTTTAGA+AGG | + | chr7.3:1328056-1328075 | MS.gene050611:intron | 20.0% |
| !!! | TCTTTTATTTTCTTCACAAA+TGG | - | chr7.3:1328334-1328353 | None:intergenic | 20.0% |
| !!! | TTCTAAAAAGTGAATTATAG+AGG | - | chr7.3:1328057-1328076 | None:intergenic | 20.0% |
| ! | AATAACATCAGACAAAATTG+GGG | - | chr7.3:1327240-1327259 | None:intergenic | 25.0% |
| ! | GTCAAATGAAATTAAAAGCA+TGG | + | chr7.3:1328430-1328449 | MS.gene050611:CDS | 25.0% |
| ! | TTATATCTTAAACACTGTGT+TGG | + | chr7.3:1328004-1328023 | MS.gene050611:intron | 25.0% |
| !! | ACCTTTAATTTCCTCATAAT+AGG | - | chr7.3:1328275-1328294 | None:intergenic | 25.0% |
| !! | CCTTTAATTTCCTCATAATA+GGG | - | chr7.3:1328274-1328293 | None:intergenic | 25.0% |
| !! | TATGTCATGTTAATTTTACG+TGG | + | chr7.3:1327722-1327741 | MS.gene050611:intron | 25.0% |
| !!! | CTTCTAGATTTTGGTTAATT+CGG | - | chr7.3:1327300-1327319 | None:intergenic | 25.0% |
| ATAACATCAGACAAAATTGG+GGG | - | chr7.3:1327239-1327258 | None:intergenic | 30.0% | |
| CCCTATTATGAGGAAATTAA+AGG | + | chr7.3:1328271-1328290 | MS.gene050611:intron | 30.0% | |
| TAGTTATCAGCAAATGAGAT+AGG | + | chr7.3:1328246-1328265 | MS.gene050611:intron | 30.0% | |
| TTAACCAAAATCTAGAAGCT+TGG | + | chr7.3:1327302-1327321 | MS.gene050611:intron | 30.0% | |
| ! | AGAATAGGAGAAAGGATTTT+TGG | - | chr7.3:1328150-1328169 | None:intergenic | 30.0% |
| ! | CATTTGCTGATAACTACAAA+AGG | - | chr7.3:1328242-1328261 | None:intergenic | 30.0% |
| ! | GAATAGGAGAAAGGATTTTT+GGG | - | chr7.3:1328149-1328168 | None:intergenic | 30.0% |
| !! | GGCTATTTTCACTTTGATAT+AGG | + | chr7.3:1327465-1327484 | MS.gene050611:intron | 30.0% |
| !! | TTTTTCAGTTTCTTCCTGTT+AGG | - | chr7.3:1328212-1328231 | None:intergenic | 30.0% |
| !!! | TCACATTCCCATTTTTAGTT+GGG | - | chr7.3:1327792-1327811 | None:intergenic | 30.0% |
| !!! | TTCACATTCCCATTTTTAGT+TGG | - | chr7.3:1327793-1327812 | None:intergenic | 30.0% |
| AAGAAGACCCCAACTAAAAA+TGG | + | chr7.3:1327781-1327800 | MS.gene050611:CDS | 35.0% | |
| AAGTGTGAGCAACAAAATAG+TGG | - | chr7.3:1328097-1328116 | None:intergenic | 35.0% | |
| AGAAGACCCCAACTAAAAAT+GGG | + | chr7.3:1327782-1327801 | MS.gene050611:CDS | 35.0% | |
| ATAGGTCAAGAATAGGAGAA+AGG | - | chr7.3:1328158-1328177 | None:intergenic | 35.0% | |
| ATGCTTTAACGCATTTCGAA+TGG | + | chr7.3:1327652-1327671 | MS.gene050611:intron | 35.0% | |
| TTCACTTTGATATAGGCAAG+AGG | + | chr7.3:1327472-1327491 | MS.gene050611:intron | 35.0% | |
| TTTCTCCTATTCTTGACCTA+TGG | + | chr7.3:1328157-1328176 | MS.gene050611:intron | 35.0% | |
| ! | AGAACCAAGCTTCTAGATTT+TGG | - | chr7.3:1327309-1327328 | None:intergenic | 35.0% |
| !! | GCTGCTTACTTTTGATATTG+TGG | + | chr7.3:1328360-1328379 | MS.gene050611:intron | 35.0% |
| !!! | CACATTCCCATTTTTAGTTG+GGG | - | chr7.3:1327791-1327810 | None:intergenic | 35.0% |
| !!! | CTGATGTCGGTTTTCTTATT+GGG | + | chr7.3:1327444-1327463 | MS.gene050611:intron | 35.0% |
| !!! | TCTGATGTCGGTTTTCTTAT+TGG | + | chr7.3:1327443-1327462 | MS.gene050611:intron | 35.0% |
| !!! | TTTTGGGTTGGAAACAAAAC+AGG | - | chr7.3:1328133-1328152 | None:intergenic | 35.0% |
| CAAGAAGGAGGAAAGTAGTT+TGG | + | chr7.3:1327581-1327600 | MS.gene050611:CDS | 40.0% | |
| CACTTTCACTTCTTCCTCAT+CGG | - | chr7.3:1327872-1327891 | None:intergenic | 40.0% | |
| GAGATAGGATCCCTATTATG+AGG | + | chr7.3:1328261-1328280 | MS.gene050611:intron | 40.0% | |
| TTAGTTGGGGTCTTCTTTGA+AGG | - | chr7.3:1327778-1327797 | None:intergenic | 40.0% | |
| TTCAGCCATAGGTCAAGAAT+AGG | - | chr7.3:1328165-1328184 | None:intergenic | 40.0% | |
| ! | AAAGTCAGATTCCTCTGAAG+TGG | - | chr7.3:1327833-1327852 | None:intergenic | 40.0% |
| !!! | AGGAGAAAGGATTTTTGGGT+TGG | - | chr7.3:1328145-1328164 | None:intergenic | 40.0% |
| AAAGAAGCAAGGGGTAGAAG+AGG | + | chr7.3:1327527-1327546 | MS.gene050611:CDS | 45.0% | |
| AGTGAGTGCAAAGAAGCAAG+GGG | + | chr7.3:1327518-1327537 | MS.gene050611:CDS | 45.0% | |
| CAGTGAGTGCAAAGAAGCAA+GGG | + | chr7.3:1327517-1327536 | MS.gene050611:CDS | 45.0% | |
| CTCTCATATCACTGCCTAAC+AGG | + | chr7.3:1328195-1328214 | MS.gene050611:intron | 45.0% | |
| CTGTTAGGCAGTGATATGAG+AGG | - | chr7.3:1328197-1328216 | None:intergenic | 45.0% | |
| CTTTGATATAGGCAAGAGGC+AGG | + | chr7.3:1327476-1327495 | MS.gene050611:intron | 45.0% | |
| GAAGGAGGAAAGTAGTTTGG+AGG | + | chr7.3:1327584-1327603 | MS.gene050611:CDS | 45.0% | |
| GGAGAGTTTCTTTCAGCCAT+AGG | - | chr7.3:1328176-1328195 | None:intergenic | 45.0% | |
| GTTGAAGGTAGTCAAGAAGG+AGG | + | chr7.3:1327569-1327588 | MS.gene050611:CDS | 45.0% | |
| TAGTTTGGAGGAATCCTCTG+AGG | + | chr7.3:1327596-1327615 | MS.gene050611:CDS | 45.0% | |
| ! | TACCTTCAACGCCTTTTGCT+TGG | - | chr7.3:1327559-1327578 | None:intergenic | 45.0% |
| CACTTCTTCCTCATCGGAAG+AGG | - | chr7.3:1327866-1327885 | None:intergenic | 50.0% | |
| CTCATCCTCAGATTCCTCAG+AGG | - | chr7.3:1327613-1327632 | None:intergenic | 50.0% | |
| GCAGTGAGTGCAAAGAAGCA+AGG | + | chr7.3:1327516-1327535 | MS.gene050611:CDS | 50.0% | |
| ! | CTCTGAGGAATCTGAGGATG+AGG | + | chr7.3:1327611-1327630 | MS.gene050611:CDS | 50.0% |
| ! | CTTCAACGCCTTTTGCTTGG+CGG | - | chr7.3:1327556-1327575 | None:intergenic | 50.0% |
| ! | GGAATCCTCTGAGGAATCTG+AGG | + | chr7.3:1327605-1327624 | MS.gene050611:CDS | 50.0% |
| ! | GGCGTTGAAGGTAGTCAAGA+AGG | + | chr7.3:1327566-1327585 | MS.gene050611:CDS | 50.0% |
| !! | GATTCCTCTGAAGTGGTCTC+TGG | - | chr7.3:1327826-1327845 | None:intergenic | 50.0% |
| AGAGGTTGCCGCCAAGCAAA+AGG | + | chr7.3:1327545-1327564 | MS.gene050611:CDS | 55.0% | |
| GCAGGCTGAGGAAGAAGTCA+AGG | + | chr7.3:1327494-1327513 | MS.gene050611:CDS | 55.0% | |
| TATAGGCAAGAGGCAGGCTG+AGG | + | chr7.3:1327482-1327501 | MS.gene050611:intron | 55.0% | |
| TGACAGCTCCTCTTCCGATG+AGG | + | chr7.3:1327855-1327874 | MS.gene050611:CDS | 55.0% | |
| ! | GGAGCAGGAGCTTTTGCAAC+AGG | - | chr7.3:1327757-1327776 | None:intergenic | 55.0% |
| ! | GGGGTCTTCTTTGAAGGAGC+AGG | - | chr7.3:1327772-1327791 | None:intergenic | 55.0% |
| !! | CGCCAAGCAAAAGGCGTTGA+AGG | + | chr7.3:1327554-1327573 | MS.gene050611:CDS | 55.0% |
| !! | CTCTGAAGTGGTCTCTGGCT+GGG | - | chr7.3:1327821-1327840 | None:intergenic | 55.0% |
| CCAGCCAGAGACCACTTCAG+AGG | + | chr7.3:1327819-1327838 | MS.gene050611:CDS | 60.0% | |
| !! | CCTCTGAAGTGGTCTCTGGC+TGG | - | chr7.3:1327822-1327841 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 1327221 | 1328455 | 1327221 | ID=MS.gene050611 |
| chr7.3 | mRNA | 1327221 | 1328455 | 1327221 | ID=MS.gene050611.t1;Parent=MS.gene050611 |
| chr7.3 | exon | 1327221 | 1327230 | 1327221 | ID=MS.gene050611.t1.exon1;Parent=MS.gene050611.t1 |
| chr7.3 | CDS | 1327221 | 1327230 | 1327221 | ID=cds.MS.gene050611.t1;Parent=MS.gene050611.t1 |
| chr7.3 | exon | 1327487 | 1327632 | 1327487 | ID=MS.gene050611.t1.exon2;Parent=MS.gene050611.t1 |
| chr7.3 | CDS | 1327487 | 1327632 | 1327487 | ID=cds.MS.gene050611.t1;Parent=MS.gene050611.t1 |
| chr7.3 | exon | 1327751 | 1327888 | 1327751 | ID=MS.gene050611.t1.exon3;Parent=MS.gene050611.t1 |
| chr7.3 | CDS | 1327751 | 1327888 | 1327751 | ID=cds.MS.gene050611.t1;Parent=MS.gene050611.t1 |
| chr7.3 | exon | 1328417 | 1328455 | 1328417 | ID=MS.gene050611.t1.exon4;Parent=MS.gene050611.t1 |
| chr7.3 | CDS | 1328417 | 1328455 | 1328417 | ID=cds.MS.gene050611.t1;Parent=MS.gene050611.t1 |
| Gene Sequence |
| Protein sequence |
