Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene48823.t1 | XP_013455173.1 | 90.5 | 264 | 21 | 4 | 9 | 268 | 119 | 382 | 5.40E-114 | 420.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene48823.t1 | Q9LZW2 | 60.0 | 105 | 32 | 3 | 161 | 264 | 167 | 262 | 4.9e-23 | 109.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene48823.t1 | I3SI32 | 90.5 | 264 | 21 | 4 | 9 | 268 | 119 | 382 | 3.9e-114 | 420.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene48823.t1 | TF | OFP |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049257 | MS.gene48823 | 0.808077 | 3.70E-50 | -1.69E-46 |
| MS.gene050553 | MS.gene48823 | 0.801645 | 8.17E-49 | -1.69E-46 |
| MS.gene055539 | MS.gene48823 | 0.816859 | 4.45E-52 | -1.69E-46 |
| MS.gene05556 | MS.gene48823 | 0.813495 | 2.49E-51 | -1.69E-46 |
| MS.gene059717 | MS.gene48823 | 0.800157 | 1.64E-48 | -1.69E-46 |
| MS.gene060717 | MS.gene48823 | 0.805453 | 1.33E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene48823.t1 | MTR_4g028930 | 91.406 | 256 | 18 | 4 | 17 | 268 | 127 | 382 | 1.47e-147 | 418 |
| MS.gene48823.t1 | MTR_7g072875 | 53.309 | 272 | 67 | 11 | 27 | 266 | 42 | 285 | 1.65e-71 | 221 |
| MS.gene48823.t1 | MTR_7g072630 | 53.309 | 272 | 67 | 11 | 27 | 266 | 104 | 347 | 2.00e-70 | 221 |
| MS.gene48823.t1 | MTR_5g075210 | 43.750 | 208 | 88 | 8 | 76 | 266 | 151 | 346 | 1.51e-41 | 146 |
| MS.gene48823.t1 | MTR_2g018030 | 40.136 | 147 | 75 | 3 | 133 | 268 | 201 | 345 | 8.86e-28 | 109 |
| MS.gene48823.t1 | MTR_8g087530 | 55.224 | 67 | 30 | 0 | 201 | 267 | 259 | 325 | 2.39e-21 | 92.0 |
| MS.gene48823.t1 | MTR_0907s0020 | 46.377 | 69 | 37 | 0 | 193 | 261 | 95 | 163 | 7.65e-16 | 73.9 |
| MS.gene48823.t1 | MTR_4g132740 | 47.761 | 67 | 35 | 0 | 198 | 264 | 79 | 145 | 4.05e-15 | 71.2 |
| MS.gene48823.t1 | MTR_8g103520 | 52.459 | 61 | 29 | 0 | 201 | 261 | 225 | 285 | 7.01e-15 | 73.2 |
| MS.gene48823.t1 | MTR_4g052040 | 49.180 | 61 | 31 | 0 | 201 | 261 | 100 | 160 | 2.51e-13 | 67.0 |
| MS.gene48823.t1 | MTR_7g105790 | 45.000 | 60 | 33 | 0 | 202 | 261 | 112 | 171 | 9.74e-13 | 65.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene48823.t1 | AT1G06920 | 51.240 | 121 | 53 | 2 | 144 | 264 | 193 | 307 | 1.55e-30 | 116 |
| MS.gene48823.t1 | AT2G30400 | 58.716 | 109 | 41 | 3 | 160 | 267 | 214 | 319 | 1.82e-29 | 113 |
| MS.gene48823.t1 | AT5G58360 | 50.847 | 118 | 46 | 3 | 149 | 266 | 185 | 290 | 3.96e-27 | 107 |
| MS.gene48823.t1 | AT5G01840 | 52.778 | 108 | 41 | 2 | 158 | 264 | 164 | 262 | 4.55e-26 | 103 |
| MS.gene48823.t1 | AT4G18830 | 59.375 | 64 | 26 | 0 | 201 | 264 | 283 | 346 | 8.59e-20 | 87.8 |
| MS.gene48823.t1 | AT5G19650 | 53.125 | 64 | 30 | 0 | 201 | 264 | 155 | 218 | 1.40e-15 | 74.3 |
| MS.gene48823.t1 | AT3G52525 | 46.154 | 65 | 35 | 0 | 199 | 263 | 65 | 129 | 1.22e-14 | 70.1 |
| MS.gene48823.t1 | AT2G18500 | 46.032 | 63 | 34 | 0 | 202 | 264 | 228 | 290 | 3.33e-13 | 68.9 |
| MS.gene48823.t1 | AT4G04025 | 46.053 | 76 | 34 | 1 | 185 | 260 | 105 | 173 | 6.70e-13 | 65.9 |
| MS.gene48823.t1 | AT2G36026 | 50.000 | 56 | 28 | 0 | 206 | 261 | 92 | 147 | 1.40e-12 | 65.1 |
Find 42 sgRNAs with CRISPR-Local
Find 75 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACACGGTCGGTCCTGAATTC+AGG | 0.231482 | 4.4:-73906389 | None:intergenic |
| TCAAGGTATTCAAGCAAATA+TGG | 0.240278 | 4.4:+73906999 | MS.gene48823:CDS |
| TGGTTTGATTTGACTGAAAC+AGG | 0.278617 | 4.4:+73907019 | MS.gene48823:CDS |
| AATATCTATGTTATTGTTGT+TGG | 0.284819 | 4.4:-73906483 | None:intergenic |
| TGATGAAACTGAACAATTCT+TGG | 0.299759 | 4.4:-73906758 | None:intergenic |
| CATAAGAGCATCAAAGGATT+TGG | 0.303549 | 4.4:+73906919 | MS.gene48823:CDS |
| TGAACCATCGTTCGATGAAA+TGG | 0.378307 | 4.4:+73906424 | MS.gene48823:CDS |
| CTTTAGAGACTCTTCTTTGA+AGG | 0.420108 | 4.4:-73906642 | None:intergenic |
| AGCTTGTAAAACCAAGCATT+GGG | 0.421130 | 4.4:-73905960 | None:intergenic |
| GTGTCATCGACAGTGAATTC+CGG | 0.429982 | 4.4:+73906794 | MS.gene48823:CDS |
| CAGATATGATTCCCAATGCT+TGG | 0.444868 | 4.4:+73905949 | MS.gene48823:CDS |
| TCGGTCCTGAATTCAGGGTC+GGG | 0.445910 | 4.4:-73906383 | None:intergenic |
| TAGCTTGTAAAACCAAGCAT+TGG | 0.457451 | 4.4:-73905961 | None:intergenic |
| AATGGTTCTGTATATTCTCC+TGG | 0.460992 | 4.4:+73906713 | MS.gene48823:CDS |
| AGAATTGTTCAGTTTCATCA+TGG | 0.500576 | 4.4:+73906761 | MS.gene48823:CDS |
| GGCTATCGGAGAAGCTTCTC+CGG | 0.501925 | 4.4:+73906815 | MS.gene48823:CDS |
| AGAAAAGATGATAAACTTGA+AGG | 0.506908 | 4.4:+73906536 | MS.gene48823:CDS |
| TTGTTCAGTTTCATCATGGC+CGG | 0.517006 | 4.4:+73906765 | MS.gene48823:CDS |
| GTCGGTCCTGAATTCAGGGT+CGG | 0.520173 | 4.4:-73906384 | None:intergenic |
| CGGTCCTGAATTCAGGGTCG+GGG | 0.520256 | 4.4:-73906382 | None:intergenic |
| TTTAGAGACTCTTCTTTGAA+GGG | 0.523785 | 4.4:-73906641 | None:intergenic |
| TTTATACGAAGTTTCACTCC+AGG | 0.530998 | 4.4:-73906731 | None:intergenic |
| CACGGTCGGTCCTGAATTCA+GGG | 0.532431 | 4.4:-73906388 | None:intergenic |
| GAATAACATAAGAGCATCAA+AGG | 0.542179 | 4.4:+73906913 | MS.gene48823:CDS |
| TCATCGAACGATGGTTCAGT+TGG | 0.543834 | 4.4:-73906419 | None:intergenic |
| GTATCATGATCTTATTATCA+AGG | 0.547052 | 4.4:+73906982 | MS.gene48823:CDS |
| TGATTTGACTGAAACAGGGT+AGG | 0.547431 | 4.4:+73907024 | MS.gene48823:CDS |
| GATTTGACTGAAACAGGGTA+GGG | 0.553104 | 4.4:+73907025 | MS.gene48823:CDS |
| CTTCACTATTGCTAGACTAC+CGG | 0.553123 | 4.4:-73906834 | None:intergenic |
| GAAAAGATGATAAACTTGAA+GGG | 0.554436 | 4.4:+73906537 | MS.gene48823:CDS |
| GGAGAAGCTTCTCCGATAGC+CGG | 0.561715 | 4.4:-73906813 | None:intergenic |
| CGACAGTGAATTCCGGCTAT+CGG | 0.576491 | 4.4:+73906801 | MS.gene48823:CDS |
| GGTCCTGAATTCAGGGTCGG+GGG | 0.578117 | 4.4:-73906381 | None:intergenic |
| GTCAATGGTGGAGATGATTG+TGG | 0.584914 | 4.4:+73906889 | MS.gene48823:CDS |
| GACTCTCTAAAATCCTTCTG+AGG | 0.593112 | 4.4:-73906869 | None:intergenic |
| TTTGTTACAAGCACACGAAG+TGG | 0.593184 | 4.4:-73906456 | None:intergenic |
| GATTCAGTTAAGCTATCAAG+TGG | 0.609478 | 4.4:-73906353 | None:intergenic |
| GAAACCATTTCATCGAACGA+TGG | 0.662343 | 4.4:-73906428 | None:intergenic |
| AGTTGGTATCAGAACACGGT+CGG | 0.669498 | 4.4:-73906402 | None:intergenic |
| GGTTTGATTTGACTGAAACA+GGG | 0.673887 | 4.4:+73907020 | MS.gene48823:CDS |
| GAACGACGCTTGAATCAGTG+TGG | 0.678886 | 4.4:+73906291 | MS.gene48823:CDS |
| GTTCAGTTGGTATCAGAACA+CGG | 0.691441 | 4.4:-73906406 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATATCTATGTTATTGTTGT+TGG | - | chr4.4:73906486-73906505 | None:intergenic | 20.0% |
| !!! | ATTTTCTAGGATTATTACAT+TGG | - | chr4.4:73906103-73906122 | None:intergenic | 20.0% |
| !!! | ATTTTTTGATGTTAATGTTG+TGG | - | chr4.4:73906216-73906235 | None:intergenic | 20.0% |
| !!! | TTTTACAAGCTAAAAGAAAT+AGG | + | chr4.4:73905972-73905991 | MS.gene48823:intron | 20.0% |
| ! | AAGAAGAACAAGAAGAAAAA+TGG | + | chr4.4:73906695-73906714 | MS.gene48823:CDS | 25.0% |
| ! | AGAAAAGATGATAAACTTGA+AGG | + | chr4.4:73906536-73906555 | MS.gene48823:CDS | 25.0% |
| ! | GAAAAGATGATAAACTTGAA+GGG | + | chr4.4:73906537-73906556 | MS.gene48823:CDS | 25.0% |
| ! | GTATCATGATCTTATTATCA+AGG | + | chr4.4:73906982-73907001 | MS.gene48823:CDS | 25.0% |
| ! | GTTGTTGAAGATAATGAAAA+AGG | - | chr4.4:73906047-73906066 | None:intergenic | 25.0% |
| !! | TCTTTTTCTTGTTCTGTTTT+TGG | - | chr4.4:73906189-73906208 | None:intergenic | 25.0% |
| !!! | AAAAGGTTGTTTTTTCTTCT+TGG | - | chr4.4:73906030-73906049 | None:intergenic | 25.0% |
| !!! | TTTGTGGTGTTTTTGTTTTT+GGG | - | chr4.4:73906160-73906179 | None:intergenic | 25.0% |
| !!! | TTTTTGTTTTTGGGTTGTTT+TGG | - | chr4.4:73906151-73906170 | None:intergenic | 25.0% |
| AGAATTGTTCAGTTTCATCA+TGG | + | chr4.4:73906761-73906780 | MS.gene48823:CDS | 30.0% | |
| TGATGAAACTGAACAATTCT+TGG | - | chr4.4:73906761-73906780 | None:intergenic | 30.0% | |
| TTTAGAGACTCTTCTTTGAA+GGG | - | chr4.4:73906644-73906663 | None:intergenic | 30.0% | |
| ! | CTAGGATTATTACATTGGTT+GGG | - | chr4.4:73906098-73906117 | None:intergenic | 30.0% |
| ! | GTGAAGTAGTAAGATTTTCT+AGG | - | chr4.4:73906116-73906135 | None:intergenic | 30.0% |
| ! | TCAAGGTATTCAAGCAAATA+TGG | + | chr4.4:73906999-73907018 | MS.gene48823:CDS | 30.0% |
| ! | TCTAGGATTATTACATTGGT+TGG | - | chr4.4:73906099-73906118 | None:intergenic | 30.0% |
| !! | GAATAACATAAGAGCATCAA+AGG | + | chr4.4:73906913-73906932 | MS.gene48823:CDS | 30.0% |
| !!! | ATGGTTGGTTTTGTAATGAT+AGG | - | chr4.4:73906605-73906624 | None:intergenic | 30.0% |
| !!! | GTTTGTGGTGTTTTTGTTTT+TGG | - | chr4.4:73906161-73906180 | None:intergenic | 30.0% |
| !!! | TTTCTTGTTCTGTTTTTGGA+TGG | - | chr4.4:73906185-73906204 | None:intergenic | 30.0% |
| ACAGAAGAGGTAACATGTTT+CGG | - | chr4.4:73906251-73906270 | None:intergenic | 35.0% | |
| AGCTTGTAAAACCAAGCATT+GGG | - | chr4.4:73905963-73905982 | None:intergenic | 35.0% | |
| GATTCAGTTAAGCTATCAAG+TGG | - | chr4.4:73906356-73906375 | None:intergenic | 35.0% | |
| TTTATACGAAGTTTCACTCC+AGG | - | chr4.4:73906734-73906753 | None:intergenic | 35.0% | |
| ! | AATGGTTCTGTATATTCTCC+TGG | + | chr4.4:73906713-73906732 | MS.gene48823:CDS | 35.0% |
| ! | CTTTAGAGACTCTTCTTTGA+AGG | - | chr4.4:73906645-73906664 | None:intergenic | 35.0% |
| ! | GAAGAAGAAGAGTTTTCATG+AGG | - | chr4.4:73906332-73906351 | None:intergenic | 35.0% |
| ! | GATTATTACATTGGTTGGGT+TGG | - | chr4.4:73906094-73906113 | None:intergenic | 35.0% |
| ! | GTCATCTTTTAATCCTCAGA+AGG | + | chr4.4:73906856-73906875 | MS.gene48823:CDS | 35.0% |
| ! | TAGCTTGTAAAACCAAGCAT+TGG | - | chr4.4:73905964-73905983 | None:intergenic | 35.0% |
| !! | CATAAGAGCATCAAAGGATT+TGG | + | chr4.4:73906919-73906938 | MS.gene48823:CDS | 35.0% |
| !! | GAAGGATTTTAGAGAGTCAA+TGG | + | chr4.4:73906874-73906893 | MS.gene48823:CDS | 35.0% |
| !! | GGTTTGATTTGACTGAAACA+GGG | + | chr4.4:73907020-73907039 | MS.gene48823:CDS | 35.0% |
| !! | TGGTTTGATTTGACTGAAAC+AGG | + | chr4.4:73907019-73907038 | MS.gene48823:CDS | 35.0% |
| !! | TTGTGCTCATTTTTCATGGT+TGG | - | chr4.4:73906620-73906639 | None:intergenic | 35.0% |
| !!! | GGTTGTTTTTTCTTCTTGGA+AGG | - | chr4.4:73906026-73906045 | None:intergenic | 35.0% |
| !!! | GTTGGTTTTGTAATGATAGG+AGG | - | chr4.4:73906602-73906621 | None:intergenic | 35.0% |
| CAGATATGATTCCCAATGCT+TGG | + | chr4.4:73905949-73905968 | MS.gene48823:CDS | 40.0% | |
| CTTCACTATTGCTAGACTAC+CGG | - | chr4.4:73906837-73906856 | None:intergenic | 40.0% | |
| GAAACCATTTCATCGAACGA+TGG | - | chr4.4:73906431-73906450 | None:intergenic | 40.0% | |
| TGAACCATCGTTCGATGAAA+TGG | + | chr4.4:73906424-73906443 | MS.gene48823:CDS | 40.0% | |
| TTGTTCAGTTTCATCATGGC+CGG | + | chr4.4:73906765-73906784 | MS.gene48823:CDS | 40.0% | |
| TTTGTTACAAGCACACGAAG+TGG | - | chr4.4:73906459-73906478 | None:intergenic | 40.0% | |
| ! | GTTCAGTTGGTATCAGAACA+CGG | - | chr4.4:73906409-73906428 | None:intergenic | 40.0% |
| !! | GACTCTCTAAAATCCTTCTG+AGG | - | chr4.4:73906872-73906891 | None:intergenic | 40.0% |
| !! | GGATTTTAGAGAGTCAATGG+TGG | + | chr4.4:73906877-73906896 | MS.gene48823:CDS | 40.0% |
| !! | GGGTTTGTGCTCATTTTTCA+TGG | - | chr4.4:73906624-73906643 | None:intergenic | 40.0% |
| !! | TGGAAGGTGTTGTTGATGTT+TGG | - | chr4.4:73906010-73906029 | None:intergenic | 40.0% |
| !! | TTACATTGGTTGGGTTGGTT+TGG | - | chr4.4:73906089-73906108 | None:intergenic | 40.0% |
| !!! | CTGTTTTTGGATGGTGTTTG+TGG | - | chr4.4:73906176-73906195 | None:intergenic | 40.0% |
| GTGTCATCGACAGTGAATTC+CGG | + | chr4.4:73906794-73906813 | MS.gene48823:CDS | 45.0% | |
| TCATCGAACGATGGTTCAGT+TGG | - | chr4.4:73906422-73906441 | None:intergenic | 45.0% | |
| TGTTACCTCTTCTGTTTCCG+CGG | + | chr4.4:73906256-73906275 | MS.gene48823:intron | 45.0% | |
| ! | AGTTGGTATCAGAACACGGT+CGG | - | chr4.4:73906405-73906424 | None:intergenic | 45.0% |
| ! | CTGTCGATGACACACTTTTC+CGG | - | chr4.4:73906787-73906806 | None:intergenic | 45.0% |
| ! | GTCAATGGTGGAGATGATTG+TGG | + | chr4.4:73906889-73906908 | MS.gene48823:CDS | 45.0% |
| !!! | GTTGGGTTGGTTTGGTTTTG+AGG | - | chr4.4:73906081-73906100 | None:intergenic | 45.0% |
| CGACAGTGAATTCCGGCTAT+CGG | + | chr4.4:73906801-73906820 | MS.gene48823:CDS | 50.0% | |
| GAACGACGCTTGAATCAGTG+TGG | + | chr4.4:73906291-73906310 | MS.gene48823:CDS | 50.0% | |
| GTTACCTCTTCTGTTTCCGC+GGG | + | chr4.4:73906257-73906276 | MS.gene48823:intron | 50.0% | |
| ACAACCCGCGGAAACAGAAG+AGG | - | chr4.4:73906264-73906283 | None:intergenic | 55.0% | |
| ACACGGTCGGTCCTGAATTC+AGG | - | chr4.4:73906392-73906411 | None:intergenic | 55.0% | |
| CACGGTCGGTCCTGAATTCA+GGG | - | chr4.4:73906391-73906410 | None:intergenic | 55.0% | |
| CGTTCGACAACTACAACCCG+CGG | - | chr4.4:73906276-73906295 | None:intergenic | 55.0% | |
| GGAGAAGCTTCTCCGATAGC+CGG | - | chr4.4:73906816-73906835 | None:intergenic | 55.0% | |
| GGCTATCGGAGAAGCTTCTC+CGG | + | chr4.4:73906815-73906834 | MS.gene48823:CDS | 55.0% | |
| GTCGGTCCTGAATTCAGGGT+CGG | - | chr4.4:73906387-73906406 | None:intergenic | 55.0% | |
| TCGGTCCTGAATTCAGGGTC+GGG | - | chr4.4:73906386-73906405 | None:intergenic | 55.0% | |
| AGTCCCCCGACCCTGAATTC+AGG | + | chr4.4:73906378-73906397 | MS.gene48823:CDS | 60.0% | |
| CGGTCCTGAATTCAGGGTCG+GGG | - | chr4.4:73906385-73906404 | None:intergenic | 60.0% | |
| GGTCCTGAATTCAGGGTCGG+GGG | - | chr4.4:73906384-73906403 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 73905924 | 73907045 | 73905924 | ID=MS.gene48823 |
| chr4.4 | mRNA | 73905924 | 73907045 | 73905924 | ID=MS.gene48823.t1;Parent=MS.gene48823 |
| chr4.4 | exon | 73905924 | 73905970 | 73905924 | ID=MS.gene48823.t1.exon1;Parent=MS.gene48823.t1 |
| chr4.4 | CDS | 73905924 | 73905970 | 73905924 | ID=cds.MS.gene48823.t1;Parent=MS.gene48823.t1 |
| chr4.4 | exon | 73906286 | 73907045 | 73906286 | ID=MS.gene48823.t1.exon2;Parent=MS.gene48823.t1 |
| chr4.4 | CDS | 73906286 | 73907045 | 73906286 | ID=cds.MS.gene48823.t1;Parent=MS.gene48823.t1 |
| Gene Sequence |
| Protein sequence |
