Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene49603.t1 | XP_003602894.2 | 66.1 | 59 | 20 | 0 | 40 | 98 | 13 | 71 | 1.30E-12 | 82.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene49603.t1 | Q0DJA3 | 53.8 | 65 | 29 | 1 | 35 | 98 | 24 | 88 | 2.7e-11 | 69.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene49603.t1 | G7J4K9 | 66.1 | 59 | 20 | 0 | 40 | 98 | 13 | 71 | 9.1e-13 | 82.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051544 | MS.gene49603 | 0.831087 | 2.04E-55 | -1.69E-46 |
| MS.gene052333 | MS.gene49603 | 0.818636 | 1.77E-52 | -1.69E-46 |
| MS.gene054093 | MS.gene49603 | 0.80773 | 4.38E-50 | -1.69E-46 |
| MS.gene054468 | MS.gene49603 | 0.800783 | 1.23E-48 | -1.69E-46 |
| MS.gene055701 | MS.gene49603 | 0.817907 | 2.58E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene49603.t1 | MTR_3g100120 | 66.102 | 59 | 20 | 0 | 40 | 98 | 11 | 69 | 4.16e-21 | 86.7 |
| MS.gene49603.t1 | MTR_3g100120 | 66.102 | 59 | 20 | 0 | 40 | 98 | 13 | 71 | 4.32e-21 | 86.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene49603.t1 | AT1G09700 | 54.237 | 59 | 27 | 0 | 40 | 98 | 10 | 68 | 4.04e-15 | 68.6 |
| MS.gene49603.t1 | AT1G09700 | 54.237 | 59 | 27 | 0 | 40 | 98 | 10 | 68 | 7.49e-15 | 68.6 |
Find 21 sgRNAs with CRISPR-Local
Find 106 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTGACCTGAAAGAAAGTTTA+TGG | 0.183803 | 5.1:-28674829 | None:intergenic |
| CTCTCCATAAACTTTCTTTC+AGG | 0.227895 | 5.1:+28674825 | MS.gene49603:CDS |
| TCTGTGCATACTCTTGCAAT+TGG | 0.403222 | 5.1:-28674757 | None:intergenic |
| CTTCCAGCACTGCTCTCAAA+TGG | 0.438969 | 5.1:+28673045 | MS.gene49603:CDS |
| ATTCCATTTGAGAGCAGTGC+TGG | 0.445915 | 5.1:-28673048 | None:intergenic |
| CACTGCTCTCAAATGGAATA+CGG | 0.478562 | 5.1:+28673052 | MS.gene49603:CDS |
| CAGAATTTCGTTGGCAACGG+CGG | 0.478941 | 5.1:+28673080 | MS.gene49603:CDS |
| TGTTATCAACGTCGTTATGC+CGG | 0.486161 | 5.1:+28673008 | MS.gene49603:CDS |
| CAAAACAACATGGTCAGTCC+TGG | 0.526124 | 5.1:+28672982 | None:intergenic |
| GCGCAGAATTTCGTTGGCAA+CGG | 0.546109 | 5.1:+28673077 | MS.gene49603:CDS |
| ACGGCAGCGCAGAATTTCGT+TGG | 0.551421 | 5.1:+28673071 | MS.gene49603:CDS |
| GTGTATACAACTACTAGTGA+AGG | 0.573758 | 5.1:+28674800 | MS.gene49603:CDS |
| GGAAGGTCGGAACTGCATGC+CGG | 0.575699 | 5.1:-28673027 | None:intergenic |
| GCAATTGGTTCTTGAACATG+TGG | 0.601920 | 5.1:-28674742 | None:intergenic |
| CATTTGAGAGCAGTGCTGGA+AGG | 0.609699 | 5.1:-28673044 | None:intergenic |
| TGAGAGCAGTGCTGGAAGGT+CGG | 0.611562 | 5.1:-28673040 | None:intergenic |
| TGTATACAACTACTAGTGAA+GGG | 0.642795 | 5.1:+28674801 | MS.gene49603:CDS |
| CTGAAAGAAAGTTTATGGAG+AGG | 0.658722 | 5.1:-28674824 | None:intergenic |
| ACGACGTTGATAACAACACC+AGG | 0.665068 | 5.1:-28673000 | None:intergenic |
| TCACTAGTAGTTGTATACAC+AGG | 0.686986 | 5.1:-28674797 | None:intergenic |
| AGTAGTTGTATACACAGGTG+CGG | 0.711484 | 5.1:-28674792 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATATATATATATATATATAT+GGG | - | chr5.1:28673423-28673404 | None:intergenic | 0.0% |
| !! | TATATATATATATATATATA+TGG | - | chr5.1:28673424-28673405 | None:intergenic | 0.0% |
| !! | CATTAATTAATTTATGTATT+TGG | + | chr5.1:28674281-28674300 | MS.gene49603:intron | 10.0% |
| !!! | ATTTGAAAATTTTTAAGATA+AGG | + | chr5.1:28673916-28673935 | MS.gene49603:intron | 10.0% |
| !! | ACAATAAAAATAGAGAAAAA+GGG | + | chr5.1:28673237-28673256 | MS.gene49603:intron | 15.0% |
| !! | CGATTATATATTATATATAG+TGG | - | chr5.1:28673896-28673915 | None:intergenic | 15.0% |
| !! | TAAAATACTTCTTAATTCAA+GGG | - | chr5.1:28673783-28673802 | None:intergenic | 15.0% |
| !! | TTAAAATACTTCTTAATTCA+AGG | - | chr5.1:28673784-28673803 | None:intergenic | 15.0% |
| !! | TTAGAATATATGCATTAATA+AGG | - | chr5.1:28674174-28674193 | None:intergenic | 15.0% |
| !!! | GAATTAAGAAGTATTTTAAA+AGG | + | chr5.1:28673785-28673804 | MS.gene49603:intron | 15.0% |
| !!! | TATAATCGATTTGAAAATTT+GGG | + | chr5.1:28673908-28673927 | MS.gene49603:intron | 15.0% |
| !!! | TTAATTTATGTATTTGGTTT+AGG | + | chr5.1:28674287-28674306 | MS.gene49603:intron | 15.0% |
| !! | AAAATACTTCTTAATTCAAG+GGG | - | chr5.1:28673782-28673801 | None:intergenic | 20.0% |
| !! | AATAAATGAATCAAATTGTG+AGG | - | chr5.1:28674657-28674676 | None:intergenic | 20.0% |
| !! | ATAAATGAATCAAATTGTGA+GGG | - | chr5.1:28674656-28674675 | None:intergenic | 20.0% |
| !! | GACAATAAAAATAGAGAAAA+AGG | + | chr5.1:28673236-28673255 | MS.gene49603:intron | 20.0% |
| !! | TTAGAATATATGCATTAGTA+CGG | - | chr5.1:28673847-28673866 | None:intergenic | 20.0% |
| !!! | AGTATTTTAAAAGGAACATT+TGG | + | chr5.1:28673794-28673813 | MS.gene49603:intron | 20.0% |
| !!! | ATCAACTTTCTTTTGTAAAT+AGG | + | chr5.1:28674350-28674369 | MS.gene49603:intron | 20.0% |
| !!! | ATTTTTTGTCAAAAGTAGAT+AGG | + | chr5.1:28673261-28673280 | MS.gene49603:intron | 20.0% |
| !!! | GTATAATCGATTTGAAAATT+TGG | + | chr5.1:28674383-28674402 | MS.gene49603:intron | 20.0% |
| !!! | GTATTTTAAAAGGAACATTT+GGG | + | chr5.1:28673795-28673814 | MS.gene49603:intron | 20.0% |
| ! | AAAGTGAAAATCACACTTTA+AGG | - | chr5.1:28673540-28673559 | None:intergenic | 25.0% |
| ! | ATAAAAGACACCTTCATAAA+AGG | + | chr5.1:28673637-28673656 | MS.gene49603:intron | 25.0% |
| ! | TATATATAATATACTCCCTC+CGG | + | chr5.1:28674224-28674243 | MS.gene49603:intron | 25.0% |
| !! | AGGGAGTATATTATATATAG+TGG | - | chr5.1:28674223-28674242 | None:intergenic | 25.0% |
| !! | GTGGTTTGAAATTACTTAAA+AGG | + | chr5.1:28673200-28673219 | MS.gene49603:intron | 25.0% |
| !! | GTTTTTGTTCCAAAACAAAA+AGG | + | chr5.1:28674144-28674163 | MS.gene49603:intron | 25.0% |
| !! | TAATATTTTACCCCTTTCTT+GGG | + | chr5.1:28674578-28674597 | MS.gene49603:intron | 25.0% |
| !! | TTAATATTTTACCCCTTTCT+TGG | + | chr5.1:28674577-28674596 | MS.gene49603:intron | 25.0% |
| !!! | AATTTTTAAGATAAGGCTGA+AGG | + | chr5.1:28673923-28673942 | MS.gene49603:intron | 25.0% |
| AAAAGGAACATTTGGGTATT+TGG | + | chr5.1:28673802-28673821 | MS.gene49603:intron | 30.0% | |
| ACACAAAAATCAACTTTCCA+AGG | + | chr5.1:28674494-28674513 | MS.gene49603:intron | 30.0% | |
| CACAAAAATCAACTTTCCAA+GGG | + | chr5.1:28674495-28674514 | MS.gene49603:intron | 30.0% | |
| GTATTTGGTCCAAAACAAAA+AGG | + | chr5.1:28673817-28673836 | MS.gene49603:intron | 30.0% | |
| TGTATACAACTACTAGTGAA+GGG | + | chr5.1:28674801-28674820 | MS.gene49603:CDS | 30.0% | |
| TTGACCTGAAAGAAAGTTTA+TGG | - | chr5.1:28674832-28674851 | None:intergenic | 30.0% | |
| TTGTCTCATGTAAATAGAAC+CGG | - | chr5.1:28674246-28674265 | None:intergenic | 30.0% | |
| ! | ATAGAAGACACCTTTTATGA+AGG | - | chr5.1:28673650-28673669 | None:intergenic | 30.0% |
| ! | CTTTCTTTTGTAAATAGGAC+CGG | + | chr5.1:28674355-28674374 | MS.gene49603:intron | 30.0% |
| !! | ACACCTTCTAAAATTGAAGT+GGG | + | chr5.1:28673300-28673319 | MS.gene49603:intron | 30.0% |
| !! | TAAGGTACACCTTTTTGTTT+TGG | - | chr5.1:28674156-28674175 | None:intergenic | 30.0% |
| !!! | TTGGGAAGCATTTTAAGATA+AGG | + | chr5.1:28674402-28674421 | MS.gene49603:intron | 30.0% |
| ACACCAACTTACTTTCATGA+AGG | - | chr5.1:28673588-28673607 | None:intergenic | 35.0% | |
| ACACCTTCATGAAAGTAAGT+TGG | + | chr5.1:28673582-28673601 | MS.gene49603:intron | 35.0% | |
| AGAGAGAGAAACTCTTAATG+TGG | + | chr5.1:28673181-28673200 | MS.gene49603:intron | 35.0% | |
| ATGAATCAAATTGTGAGGGT+TGG | - | chr5.1:28674652-28674671 | None:intergenic | 35.0% | |
| ATTGTAATAACGCACAACAC+AGG | - | chr5.1:28673127-28673146 | None:intergenic | 35.0% | |
| CTCTCCATAAACTTTCTTTC+AGG | + | chr5.1:28674825-28674844 | MS.gene49603:CDS | 35.0% | |
| CTGAAAGAAAGTTTATGGAG+AGG | - | chr5.1:28674827-28674846 | None:intergenic | 35.0% | |
| CTTCTAATGACGAATGTTAG+TGG | + | chr5.1:28674541-28674560 | MS.gene49603:intron | 35.0% | |
| GTGTATACAACTACTAGTGA+AGG | + | chr5.1:28674800-28674819 | MS.gene49603:CDS | 35.0% | |
| TCACTAGTAGTTGTATACAC+AGG | - | chr5.1:28674800-28674819 | None:intergenic | 35.0% | |
| TGTCAAAAGTAGATAGGTGT+AGG | + | chr5.1:28673267-28673286 | MS.gene49603:intron | 35.0% | |
| TGTGTTGTGCGTTATTACAA+TGG | + | chr5.1:28673126-28673145 | MS.gene49603:intron | 35.0% | |
| TGTTAAAAGACACCACCAAA+GGG | - | chr5.1:28673510-28673529 | None:intergenic | 35.0% | |
| TTGTTAAAAGACACCACCAA+AGG | - | chr5.1:28673511-28673530 | None:intergenic | 35.0% | |
| ! | ACACCCACTTCAATTTTAGA+AGG | - | chr5.1:28673306-28673325 | None:intergenic | 35.0% |
| !! | CACACCTTCTAAAATTGAAG+TGG | + | chr5.1:28673299-28673318 | MS.gene49603:intron | 35.0% |
| !! | TACGGTACACCTTTTTGTTT+TGG | - | chr5.1:28673829-28673848 | None:intergenic | 35.0% |
| !!! | GCATTTTAAGATAAGGCTGA+AGG | + | chr5.1:28674409-28674428 | MS.gene49603:intron | 35.0% |
| AAATCAACTTTCCAAGGGCT+TGG | + | chr5.1:28674500-28674519 | MS.gene49603:intron | 40.0% | |
| AGGATTCAAACCCAAGAAAG+GGG | - | chr5.1:28674591-28674610 | None:intergenic | 40.0% | |
| AGTAGTTGTATACACAGGTG+CGG | - | chr5.1:28674795-28674814 | None:intergenic | 40.0% | |
| CAAATCGATTATACTCCCTC+CGG | - | chr5.1:28674377-28674396 | None:intergenic | 40.0% | |
| CACTGCTCTCAAATGGAATA+CGG | + | chr5.1:28673052-28673071 | MS.gene49603:CDS | 40.0% | |
| CTCATGTAAATAGAACCGGA+GGG | - | chr5.1:28674242-28674261 | None:intergenic | 40.0% | |
| GAAGGTTTGAGAAGCAAGTT+TGG | + | chr5.1:28674427-28674446 | MS.gene49603:intron | 40.0% | |
| GAGGATTCAAACCCAAGAAA+GGG | - | chr5.1:28674592-28674611 | None:intergenic | 40.0% | |
| GCAAGTTTGGATTTCTTCCA+AGG | + | chr5.1:28674440-28674459 | MS.gene49603:intron | 40.0% | |
| TCTCATGTAAATAGAACCGG+AGG | - | chr5.1:28674243-28674262 | None:intergenic | 40.0% | |
| TCTGTGCATACTCTTGCAAT+TGG | - | chr5.1:28674760-28674779 | None:intergenic | 40.0% | |
| TGAGCTAAGGGAAAATGAGT+TGG | - | chr5.1:28674614-28674633 | None:intergenic | 40.0% | |
| TGTGTGTGTGTGTGTGTATA+GGG | - | chr5.1:28673341-28673360 | None:intergenic | 40.0% | |
| TGTTATCAACGTCGTTATGC+CGG | + | chr5.1:28673008-28673027 | MS.gene49603:CDS | 40.0% | |
| ! | CTTTTGTAAATAGGACCGGA+GGG | + | chr5.1:28674359-28674378 | MS.gene49603:intron | 40.0% |
| ! | GCAATTGGTTCTTGAACATG+TGG | - | chr5.1:28674745-28674764 | None:intergenic | 40.0% |
| ! | TCTTTTGTAAATAGGACCGG+AGG | + | chr5.1:28674358-28674377 | MS.gene49603:intron | 40.0% |
| ! | TTTTGTTACAAGCCACCCTT+TGG | + | chr5.1:28673492-28673511 | MS.gene49603:intron | 40.0% |
| AACTAGTAGCACATGCCACA+TGG | - | chr5.1:28674471-28674490 | None:intergenic | 45.0% | |
| ATCAAATTGCTCCAAGCCCT+TGG | - | chr5.1:28674514-28674533 | None:intergenic | 45.0% | |
| ATTCCATTTGAGAGCAGTGC+TGG | - | chr5.1:28673051-28673070 | None:intergenic | 45.0% | |
| GGAGGATTCAAACCCAAGAA+AGG | - | chr5.1:28674593-28674612 | None:intergenic | 45.0% | |
| GTGTGTGTGTGTGTGTATAG+GGG | - | chr5.1:28673340-28673359 | None:intergenic | 45.0% | |
| GTGTGTGTGTGTGTGTGTAT+AGG | - | chr5.1:28673342-28673361 | None:intergenic | 45.0% | |
| TAAAAGACACCACCAAAGGG+TGG | - | chr5.1:28673507-28673526 | None:intergenic | 45.0% | |
| ! | AGCTCAGCTAGTTTGAGCTA+AGG | - | chr5.1:28674627-28674646 | None:intergenic | 45.0% |
| ! | GCTAAGGGAAAATGAGTTGG+AGG | - | chr5.1:28674611-28674630 | None:intergenic | 45.0% |
| ! | GCTCAGCTAGTTTGAGCTAA+GGG | - | chr5.1:28674626-28674645 | None:intergenic | 45.0% |
| !! | ACGACGTTGATAACAACACC+AGG | - | chr5.1:28673003-28673022 | None:intergenic | 45.0% |
| AGTAGCACATGCCACATGGT+AGG | - | chr5.1:28674467-28674486 | None:intergenic | 50.0% | |
| ATTACAATGGAGTGAGCGCG+TGG | + | chr5.1:28673139-28673158 | MS.gene49603:intron | 50.0% | |
| CAGAATTTCGTTGGCAACGG+CGG | + | chr5.1:28673080-28673099 | MS.gene49603:CDS | 50.0% | |
| CTTCCAGCACTGCTCTCAAA+TGG | + | chr5.1:28673045-28673064 | MS.gene49603:CDS | 50.0% | |
| GCGCAGAATTTCGTTGGCAA+CGG | + | chr5.1:28673077-28673096 | MS.gene49603:CDS | 50.0% | |
| TCTTCCAAGGTCCTACCATG+TGG | + | chr5.1:28674453-28674472 | MS.gene49603:intron | 50.0% | |
| TGTTACAAGCCACCCTTTGG+TGG | + | chr5.1:28673495-28673514 | MS.gene49603:intron | 50.0% | |
| TTACAATGGAGTGAGCGCGT+GGG | + | chr5.1:28673140-28673159 | MS.gene49603:intron | 50.0% | |
| ! | CATTTGAGAGCAGTGCTGGA+AGG | - | chr5.1:28673047-28673066 | None:intergenic | 50.0% |
| ACGGCAGCGCAGAATTTCGT+TGG | + | chr5.1:28673071-28673090 | MS.gene49603:CDS | 55.0% | |
| CATGCCACATGGTAGGACCT+TGG | - | chr5.1:28674460-28674479 | None:intergenic | 55.0% | |
| ! | TGAGAGCAGTGCTGGAAGGT+CGG | - | chr5.1:28673043-28673062 | None:intergenic | 55.0% |
| CGGCGCGTAGCTGGAGATTT+GGG | + | chr5.1:28673100-28673119 | MS.gene49603:intron | 60.0% | |
| GGAAGGTCGGAACTGCATGC+CGG | - | chr5.1:28673030-28673049 | None:intergenic | 60.0% | |
| GCGGCGCGTAGCTGGAGATT+TGG | + | chr5.1:28673099-28673118 | MS.gene49603:intron | 65.0% | |
| TGGCAACGGCGGCGCGTAGC+TGG | + | chr5.1:28673091-28673110 | MS.gene49603:intron | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.1 | gene | 28672991 | 28674907 | 28672991 | ID=MS.gene49603 |
| chr5.1 | mRNA | 28672991 | 28674907 | 28672991 | ID=MS.gene49603.t1;Parent=MS.gene49603 |
| chr5.1 | exon | 28672991 | 28673105 | 28672991 | ID=MS.gene49603.t1.exon1;Parent=MS.gene49603.t1 |
| chr5.1 | CDS | 28672991 | 28673105 | 28672991 | ID=cds.MS.gene49603.t1;Parent=MS.gene49603.t1 |
| chr5.1 | exon | 28674729 | 28674907 | 28674729 | ID=MS.gene49603.t1.exon2;Parent=MS.gene49603.t1 |
| chr5.1 | CDS | 28674729 | 28674907 | 28674729 | ID=cds.MS.gene49603.t1;Parent=MS.gene49603.t1 |
| Gene Sequence |
| Protein sequence |
