Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene49861.t1 | XP_018459315.1 | 46.8 | 340 | 156 | 6 | 1 | 338 | 1 | 317 | 7.00E-66 | 261.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene49861.t1 | A0A2N9I863 | 41.5 | 354 | 173 | 8 | 1 | 339 | 1 | 335 | 9.9e-62 | 246.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049378 | MS.gene49861 | 0.819283 | 1.26E-52 | -1.69E-46 |
| MS.gene050227 | MS.gene49861 | 0.805507 | 1.29E-49 | -1.69E-46 |
| MS.gene050228 | MS.gene49861 | 0.817576 | 3.07E-52 | -1.69E-46 |
| MS.gene055701 | MS.gene49861 | 0.814768 | 1.30E-51 | -1.69E-46 |
| MS.gene05761 | MS.gene49861 | 0.80516 | 1.53E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene49861.t1 | MTR_7g062720 | 40.541 | 148 | 80 | 3 | 1 | 146 | 1 | 142 | 5.14e-31 | 115 |
| MS.gene49861.t1 | MTR_4g037355 | 40.441 | 136 | 61 | 4 | 155 | 290 | 1 | 116 | 1.16e-21 | 89.7 |
| MS.gene49861.t1 | MTR_5g061150 | 35.354 | 99 | 64 | 0 | 52 | 150 | 38 | 136 | 4.20e-18 | 80.1 |
| MS.gene49861.t1 | MTR_4g112730 | 57.971 | 69 | 28 | 1 | 143 | 211 | 2 | 69 | 7.91e-18 | 78.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 0 sgRNAs with CRISPR-Local
Find 90 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTCAAAATTTTAAGCATAAC+TGG | - | 15130:124205-124224 | MS.gene49861:CDS | 20.0% |
| ! | ACAAAATTAAGGCAAGATTT+GGG | - | 15130:124434-124453 | MS.gene49861:CDS | 25.0% |
| ! | ATTTACTGAATACTCTGATT+TGG | - | 15130:124678-124697 | MS.gene49861:CDS | 25.0% |
| ! | CAATCAAACAAAAATCATCA+GGG | - | 15130:124961-124980 | MS.gene49861:CDS | 25.0% |
| ! | TAAAAGATACAAAGAATCTG+TGG | - | 15130:125095-125114 | MS.gene49861:CDS | 25.0% |
| ! | TATAACTGAAAAGGAAAAGT+TGG | - | 15130:124591-124610 | MS.gene49861:CDS | 25.0% |
| !! | ATTGCACAATTAGAATTTTG+TGG | - | 15130:124562-124581 | MS.gene49861:CDS | 25.0% |
| !! | TATCTTTTACATAGATACTC+TGG | + | 15130:125084-125103 | None:intergenic | 25.0% |
| !!! | CAACAACAGTTTTATAATCT+TGG | + | 15130:124516-124535 | None:intergenic | 25.0% |
| !!! | TGTTGAAGGAGATTTTAATA+TGG | - | 15130:125194-125213 | MS.gene49861:CDS | 25.0% |
| AAAAGATCCCAAGATCTTAT+GGG | - | 15130:124411-124430 | MS.gene49861:CDS | 30.0% | |
| AACAAAAATCATCAGGGTAA+AGG | - | 15130:124967-124986 | MS.gene49861:CDS | 30.0% | |
| CCAATCAAACAAAAATCATC+AGG | - | 15130:124960-124979 | MS.gene49861:CDS | 30.0% | |
| GAAGAGGTTATATGAATTAC+AGG | - | 15130:124923-124942 | MS.gene49861:CDS | 30.0% | |
| GACAAAATTAAGGCAAGATT+TGG | - | 15130:124433-124452 | MS.gene49861:CDS | 30.0% | |
| GATCTTATGGGACAAAATTA+AGG | - | 15130:124423-124442 | MS.gene49861:CDS | 30.0% | |
| TGGATCAATGGAACAAATTA+AGG | - | 15130:124488-124507 | MS.gene49861:CDS | 30.0% | |
| TGGCAAAACTATAACTGAAA+AGG | - | 15130:124582-124601 | MS.gene49861:CDS | 30.0% | |
| TTCCATTGATCCATTAATGA+GGG | + | 15130:124481-124500 | None:intergenic | 30.0% | |
| TTGTGCAATAATTTGATGCA+TGG | + | 15130:124551-124570 | None:intergenic | 30.0% | |
| TTTGTAATCCATCATCAATG+TGG | + | 15130:124375-124394 | None:intergenic | 30.0% | |
| ! | AATTTTGTCCCATAAGATCT+TGG | + | 15130:124422-124441 | None:intergenic | 30.0% |
| ! | ATTTTGTCCCATAAGATCTT+GGG | + | 15130:124421-124440 | None:intergenic | 30.0% |
| ! | TTTGATTGGTCATATTTGAG+AGG | + | 15130:124949-124968 | None:intergenic | 30.0% |
| !! | CCTGATGATTTTTGTTTGAT+TGG | + | 15130:124963-124982 | None:intergenic | 30.0% |
| AAAGGTAAAAACATCCAGGA+AGG | - | 15130:124985-125004 | MS.gene49861:CDS | 35.0% | |
| AAAGTTGTGGCGTTTATTTC+AGG | + | 15130:124787-124806 | None:intergenic | 35.0% | |
| AATTGAGTATCTTGCATGTG+AGG | - | 15130:124258-124277 | MS.gene49861:CDS | 35.0% | |
| AGATACAAAGAATCTGTGGA+TGG | - | 15130:125099-125118 | MS.gene49861:CDS | 35.0% | |
| ATTCTTGAGGGAGAAGATTT+GGG | - | 15130:124292-124311 | MS.gene49861:CDS | 35.0% | |
| ATTGAGTATCTTGCATGTGA+GGG | - | 15130:124259-124278 | MS.gene49861:CDS | 35.0% | |
| CAAAAGATCCCAAGATCTTA+TGG | - | 15130:124410-124429 | MS.gene49861:CDS | 35.0% | |
| GTTCCATTGATCCATTAATG+AGG | + | 15130:124482-124501 | None:intergenic | 35.0% | |
| TGTTATAGATGCGGAAAGAA+AGG | - | 15130:125033-125052 | MS.gene49861:CDS | 35.0% | |
| ! | AACGCCACAACTTTTAATCA+AGG | - | 15130:124793-124812 | MS.gene49861:CDS | 35.0% |
| ! | ACCATCATCATGATTTCTAG+AGG | + | 15130:125013-125032 | None:intergenic | 35.0% |
| ! | ACGCCACAACTTTTAATCAA+GGG | - | 15130:124794-124813 | MS.gene49861:CDS | 35.0% |
| ! | ATTTTGTTCAGCCACTAGAA+GGG | + | 15130:124710-124729 | None:intergenic | 35.0% |
| ! | CCATCATCATGATTTCTAGA+GGG | + | 15130:125012-125031 | None:intergenic | 35.0% |
| ! | CTCAATTAAGTTGTTGTTCC+AGG | + | 15130:124245-124264 | None:intergenic | 35.0% |
| ! | GATGATGGTTGTTATAGATG+CGG | - | 15130:125024-125043 | MS.gene49861:CDS | 35.0% |
| ! | TATTTTGTTCAGCCACTAGA+AGG | + | 15130:124711-124730 | None:intergenic | 35.0% |
| ! | TTTTCTTTGCCAATTCCTCT+GGG | + | 15130:124324-124343 | None:intergenic | 35.0% |
| ! | TTTTTCTTTGCCAATTCCTC+TGG | + | 15130:124325-124344 | None:intergenic | 35.0% |
| !! | AGTCTTATTGCCCTCATTAA+TGG | - | 15130:124468-124487 | MS.gene49861:CDS | 35.0% |
| AATCTGTGGATGGAAAGGAA+AGG | - | 15130:125109-125128 | MS.gene49861:CDS | 40.0% | |
| ACGCCCTTGATTAAAAGTTG+TGG | + | 15130:124800-124819 | None:intergenic | 40.0% | |
| ATCAAGCACCACATTGATGA+TGG | - | 15130:124364-124383 | MS.gene49861:CDS | 40.0% | |
| ATCTGTGGATGGAAAGGAAA+GGG | - | 15130:125110-125129 | MS.gene49861:CDS | 40.0% | |
| CAAAGAATCTGTGGATGGAA+AGG | - | 15130:125104-125123 | MS.gene49861:CDS | 40.0% | |
| GATTCTTGAGGGAGAAGATT+TGG | - | 15130:124291-124310 | MS.gene49861:CDS | 40.0% | |
| GCAATATCACTTGAGATGCA+TGG | + | 15130:124636-124655 | None:intergenic | 40.0% | |
| GGACTCGATAAGATTCTTGA+GGG | - | 15130:124280-124299 | MS.gene49861:CDS | 40.0% | |
| GGGTAAAGGTAAAAACATCC+AGG | - | 15130:124981-125000 | MS.gene49861:CDS | 40.0% | |
| ! | ACAACTTTTAATCAAGGGCG+TGG | - | 15130:124799-124818 | MS.gene49861:CDS | 40.0% |
| ! | AGACAGGTAGTGTCATTCTT+TGG | + | 15130:125159-125178 | None:intergenic | 40.0% |
| ! | CCCTCTAGAAATCATGATGA+TGG | - | 15130:125009-125028 | MS.gene49861:CDS | 40.0% |
| ! | TGCCCTCATTAATGGATCAA+TGG | - | 15130:124476-124495 | MS.gene49861:CDS | 40.0% |
| AAAATCAGCAGCCTCAAGAC+AGG | + | 15130:125175-125194 | None:intergenic | 45.0% | |
| ATGCGGAAAGAAAGGCCATT+GGG | - | 15130:125041-125060 | MS.gene49861:CDS | 45.0% | |
| GAATGACACTACCTGTCTTG+AGG | - | 15130:125161-125180 | MS.gene49861:CDS | 45.0% | |
| GGATACGACATTGTTCCTGT+AGG | + | 15130:124766-124785 | None:intergenic | 45.0% | |
| GGGACTCGATAAGATTCTTG+AGG | - | 15130:124279-124298 | MS.gene49861:CDS | 45.0% | |
| GGTGGTTATAATCGCCAAAG+AGG | - | 15130:124820-124839 | MS.gene49861:CDS | 45.0% | |
| TATAATCGCCAAAGAGGACG+TGG | - | 15130:124826-124845 | MS.gene49861:CDS | 45.0% | |
| ! | CATGGTCGAAACCATTTTCG+TGG | - | 15130:124889-124908 | MS.gene49861:CDS | 45.0% |
| !! | ACTTTTAATCAAGGGCGTGG+TGG | - | 15130:124802-124821 | MS.gene49861:CDS | 45.0% |
| !!! | GAGGCTGCTGATTTTGTTGA+AGG | - | 15130:125180-125199 | MS.gene49861:CDS | 45.0% |
| AACCATCAGTTGCGTCCTAC+AGG | - | 15130:124748-124767 | MS.gene49861:CDS | 50.0% | |
| AGATTTGGGAGATGTCCCAG+AGG | - | 15130:124306-124325 | MS.gene49861:CDS | 50.0% | |
| CGTGGACGAGGTAGAAATCA+TGG | - | 15130:124871-124890 | MS.gene49861:CDS | 50.0% | |
| CTGGCGACAACTACATAACC+TGG | - | 15130:124224-124243 | MS.gene49861:CDS | 50.0% | |
| GATGCGGAAAGAAAGGCCAT+TGG | - | 15130:125040-125059 | MS.gene49861:CDS | 50.0% | |
| TCCTGCATGCTTTAGCCCAA+TGG | + | 15130:125059-125078 | None:intergenic | 50.0% | |
| TTCCTGTAGGACGCAACTGA+TGG | + | 15130:124753-124772 | None:intergenic | 50.0% | |
| TTTGGTTGCAGCCCTTCTAG+TGG | - | 15130:124696-124715 | MS.gene49861:CDS | 50.0% | |
| ! | CGAAACCATTTTCGTGGTGG+AGG | - | 15130:124895-124914 | MS.gene49861:CDS | 50.0% |
| ! | GATTTCTAGAGGGACCTTCC+TGG | + | 15130:125002-125021 | None:intergenic | 50.0% |
| ! | GGTCGAAACCATTTTCGTGG+TGG | - | 15130:124892-124911 | MS.gene49861:CDS | 50.0% |
| !! | TATGCTCGTTTTGGTGGTCG+TGG | - | 15130:124853-124872 | MS.gene49861:CDS | 50.0% |
| AATCGCCAAAGAGGACGTGG+TGG | - | 15130:124829-124848 | MS.gene49861:CDS | 55.0% | |
| CACGACCTCCACCACGAAAA+TGG | + | 15130:124903-124922 | None:intergenic | 55.0% | |
| CATAGCCACCACGTCCTCTT+TGG | + | 15130:124837-124856 | None:intergenic | 55.0% | |
| GCCATTGGGCTAAAGCATGC+AGG | - | 15130:125055-125074 | MS.gene49861:CDS | 55.0% | |
| GGGAGATGTCCCAGAGGAAT+TGG | - | 15130:124312-124331 | MS.gene49861:CDS | 55.0% | |
| ! | CGTGGTGGCTATGCTCGTTT+TGG | - | 15130:124844-124863 | MS.gene49861:CDS | 55.0% |
| ! | GGTGGCTATGCTCGTTTTGG+TGG | - | 15130:124847-124866 | MS.gene49861:CDS | 55.0% |
| !! | CATTTTCGTGGTGGAGGTCG+TGG | - | 15130:124901-124920 | MS.gene49861:CDS | 55.0% |
| !! | CGTTTTGGTGGTCGTGGACG+AGG | - | 15130:124859-124878 | MS.gene49861:CDS | 60.0% |
| ! | CGTGGTGGAGGTCGTGGAAG+AGG | - | 15130:124907-124926 | MS.gene49861:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 15130 | gene | 124184 | 125221 | 124184 | ID=MS.gene49861 |
| 15130 | mRNA | 124184 | 125221 | 124184 | ID=MS.gene49861.t1;Parent=MS.gene49861 |
| 15130 | exon | 124184 | 125221 | 124184 | ID=MS.gene49861.t1.exon1;Parent=MS.gene49861.t1 |
| 15130 | CDS | 124184 | 125221 | 124184 | ID=cds.MS.gene49861.t1;Parent=MS.gene49861.t1 |
| Gene Sequence |
| Protein sequence |
